ORF
Human Ortholog
Description Putative protein of unknown function; conserved across S. cerevisiae strains; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.96 0.97 0.9 0.8 0.75 0.69 0.69 0.66 0.63 0.98 0.99 0.99 0.93 0.94 0.96 0.97 0.97 0.89 0.95 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.09 0 0.23 0.29 0.32 0.49 0.48 0.52 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0.08 0 0 0.05 0 0 0 0.11 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 2 1 1 3 2 2 7 6 3 7 12 8 0 1 0 1 0 0 5 1 1 1 2 9
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 2 4 5 1 2 2 4 0 1 0 5 10 4 0 0 0 0 0 0
Cytoplasm 475 201 68 302 419 363 540 600 301 339 294 353 424 516 597 122 163 178 473 195 79 93 384 513
Endoplasmic Reticulum 0 0 0 1 1 1 1 0 0 1 1 0 3 1 4 4 5 8 0 0 0 0 1 2
Endosome 1 0 0 1 1 1 0 4 1 1 1 1 0 0 0 1 0 1 0 0 2 1 1 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 0 0 2 2
Mitochondria 1 0 5 27 14 91 196 255 213 238 232 302 2 1 0 2 3 0 1 1 0 0 2 9
Nucleus 2 1 1 0 2 0 7 15 9 6 11 8 1 1 1 2 0 3 1 0 0 0 2 0
Nuclear Periphery 1 0 0 0 0 0 4 0 3 0 4 5 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 4 0 0 1 4 1 1 3 1 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1 3
Vac/Vac Membrane 1 1 0 3 15 6 28 69 33 19 19 27 1 1 2 14 13 7 0 2 0 0 4 6
Unique Cell Count 478 202 70 315 432 402 673 803 438 491 448 560 431 521 605 131 174 185 489 202 89 99 407 563
Labelled Cell Count 483 205 75 341 456 467 788 960 566 616 581 710 433 522 605 151 195 202 489 202 89 99 407 563


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 7.4 4.1 5.1 5.6 4.5 4.3 4.5 4.0 4.1 3.9 3.9 7.0 7.5 7.0 8.6 8.9 8.3 6.2 6.8 6.7
Std Deviation (1e-4) 1.0 2.7 1.3 1.3 1.4 1.1 1.2 1.0 1.2 0.8 1.2 0.8 1.5 1.5 1.3 1.5 1.8 1.4 1.2 1.3 1.4
Intensity Change (Log2) – – – 0.31 0.45 0.13 0.08 0.13 -0.04 -0.01 -0.06 -0.09 0.76 0.87 0.78 1.06 1.11 1.03 0.6 0.73 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.1 -1.9 -3.5 -4.2 -5.0 -4.9 -5.3 -5.7 0.7 1.4 1.0 -1.2 -1.1 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7576 4.6701 4.1253 3.0293 3.373 3.2545 1.8016 4.2424 3.6873 3.2008 3.2941 3.2387 3.7534 5.9889 5.9878 6.1639 4.8402 5.2726
Actin 0.0144 0.0002 0.0106 0.0004 0.0001 0.0029 0.0167 0.0005 0.0285 0.0048 0.0007 0.0054 0.0047 0.0005 0.0209 0.0071 0.0001 0.0125
Bud 0.0017 0.0002 0.0002 0.0002 0.0001 0.0001 0.0006 0.0002 0.0011 0.0011 0.0004 0.0001 0.0007 0.0005 0.0006 0.0005 0 0.0002
Bud Neck 0.0008 0.0003 0.0077 0.0003 0.0002 0.0021 0.001 0.0002 0.0025 0.0011 0.0004 0.0009 0.0009 0.0002 0.002 0.0009 0.0003 0.001
Bud Periphery 0.0004 0.0001 0.0002 0.0001 0 0 0.0006 0.0001 0.0013 0.001 0.0004 0.0001 0.0003 0.0002 0.0004 0.0005 0 0.0001
Bud Site 0.001 0.0013 0.0007 0.0002 0.0001 0.0001 0.0033 0.0009 0.0057 0.0059 0.0001 0.0001 0.0014 0.0034 0.0078 0.0017 0 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0002 0.0001 0 0 0.0001 0.0001 0.0002 0.0002 0 0
Cytoplasm 0.8494 0.9461 0.9171 0.9227 0.915 0.9333 0.7021 0.9335 0.8697 0.7589 0.914 0.9416 0.787 0.9328 0.8764 0.8109 0.9892 0.8921
Cytoplasmic Foci 0.0121 0.0046 0.0032 0.0039 0.0182 0.0088 0.0365 0.0043 0.0095 0.0637 0.0033 0.0116 0.0302 0.0048 0.0109 0.0288 0.0015 0.0087
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0 0 0 0 0.0001 0.0003 0 0.0002
Endoplasmic Reticulum 0.0069 0.0018 0.0029 0.0025 0.0049 0.0023 0.0068 0.0015 0.0036 0.0024 0.0015 0.0018 0.0033 0.0012 0.0015 0.0016 0.0004 0.0021
Endosome 0.0072 0.0025 0.0052 0.0019 0.0176 0.0034 0.0379 0.0016 0.0115 0.0491 0.0134 0.0073 0.0066 0.0007 0.0122 0.0115 0.0003 0.013
Golgi 0.0008 0.0001 0.0016 0.0001 0.0002 0.0003 0.0082 0.0001 0.0032 0.0118 0.0085 0.0007 0.0056 0.0002 0.0086 0.0064 0 0.0133
Lipid Particles 0.0025 0.0001 0.0018 0 0.0002 0.0005 0.0148 0 0.0019 0.004 0.0008 0.0001 0.0402 0 0.006 0.011 0 0.0019
Mitochondria 0.0004 0.0002 0.0009 0.0002 0.0003 0.0002 0.0098 0.0001 0.0054 0.0076 0.024 0.0003 0.002 0.0002 0.0088 0.0079 0 0.003
None 0.0851 0.0339 0.0278 0.0584 0.0342 0.0311 0.1197 0.0508 0.0397 0.0096 0.0158 0.0069 0.0695 0.0473 0.0167 0.0644 0.0029 0.0371
Nuclear Periphery 0.0028 0.0015 0.0044 0.0014 0.0014 0.0016 0.0054 0.0008 0.0022 0.0029 0.0017 0.0016 0.0015 0.0006 0.0019 0.0034 0.0004 0.0012
Nucleolus 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0008 0.0001 0.0002 0.0003 0 0 0.0001 0 0.0007 0.0014 0 0.0001
Nucleus 0.0051 0.0047 0.0122 0.0061 0.0035 0.0091 0.0068 0.0038 0.0053 0.0124 0.0104 0.0142 0.0031 0.0052 0.0112 0.0072 0.0044 0.0104
Peroxisomes 0.0019 0.0001 0.0005 0.0001 0.0001 0.0004 0.0147 0.0001 0.0048 0.0523 0.0001 0.0049 0.0401 0.0007 0.0075 0.0289 0 0.0015
Punctate Nuclear 0.005 0.0009 0.002 0.0006 0.001 0.0028 0.0068 0.0006 0.002 0.0055 0.0008 0.0012 0.0015 0.0006 0.0024 0.0029 0.0002 0.0009
Vacuole 0.0019 0.001 0.0007 0.0006 0.0026 0.0007 0.0058 0.0006 0.0014 0.0038 0.0017 0.001 0.0009 0.0005 0.0028 0.0016 0.0001 0.0005
Vacuole Periphery 0.0003 0.0001 0.0002 0.0001 0.0005 0.0002 0.0012 0.0001 0.0003 0.0015 0.0019 0.0002 0.0003 0.0001 0.0004 0.0008 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
171 1127 1603 205 974 276 1175 1278 1145 1403 2778 1483

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 703.55 830.07 996.32 1004.36 737.22 898.38 1028.67 967.01 732.19 843.51 1010.00 972.17
Standard Deviation 93.51 123.96 148.65 137.49 94.46 115.32 144.78 129.10 95.08 125.29 147.89 130.92
Intensity Change Log 2 — 0.238580 0.501956 0.513552 — 0.285231 0.480613 0.391436 — 0.262639 0.491075 0.452359

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000147 0.000291 0.000228 0.000212 0.000114 0.000706 0.000496 0.001412 0.000119 0.000373 0.000341 0.001246
Bud Neck 0.015568 0.009783 0.002566 0.002652 0.007014 0.014252 0.003949 0.005868 0.008292 0.010662 0.003151 0.005424
Bud Site 0.005662 0.015824 0.003795 0.023693 0.004914 0.013105 0.014517 0.023291 0.005026 0.015289 0.008330 0.023347
Cell Periphery 0.000220 0.000089 0.000085 0.000155 0.000127 0.000117 0.000084 0.000119 0.000141 0.000095 0.000084 0.000124
Cytoplasm 0.472910* 0.698428* 0.531973* 0.556714* 0.564989* 0.457041* 0.592405* 0.545959* 0.551237* 0.650942* 0.557534* 0.547446*
Cytoplasmic Foci 0.298410* 0.113863 0.004666 0.014167 0.239999* 0.185357* 0.005969 0.034151 0.248722* 0.127927 0.005217 0.031388
Eisosomes 0.000220 0.000036 0.000013 0.000049 0.000103 0.000081 0.000016 0.000035 0.000121 0.000045 0.000014 0.000037
Endoplasmic Reticulum 0.000822 0.000665 0.001770 0.001777 0.000914 0.000683 0.001360 0.001065 0.000901 0.000669 0.001596 0.001164
Endosome 0.011990 0.009958 0.000701 0.002838 0.010882 0.032883 0.001029 0.020043 0.011047 0.014468 0.000840 0.017665
Golgi 0.001979 0.002406 0.000069 0.000112 0.003148 0.006591 0.000098 0.014890 0.002973 0.003230 0.000081 0.012847
Lipid Particles 0.007757 0.001064 0.000459 0.001063 0.005981 0.004651 0.000569 0.000972 0.006246 0.001770 0.000506 0.000985
Mitochondria 0.001909 0.001161 0.000364 0.000187 0.003206 0.013599 0.001693 0.019458 0.003012 0.003608 0.000926 0.016794
Mitotic Spindle 0.000132 0.009119 0.004240 0.006795 0.001517 0.011925 0.013516 0.060378 0.001310 0.009671 0.008164 0.052971
None 0.002617 0.004370 0.007247 0.008456 0.003223 0.000693 0.006882 0.010080 0.003132 0.003647 0.007093 0.009856
Nuclear Periphery 0.000236 0.000180 0.000811 0.000902 0.000967 0.000306 0.000835 0.000896 0.000858 0.000205 0.000821 0.000897
Nuclear Periphery Foci 0.000729 0.001209 0.001398 0.002231 0.000778 0.000557 0.001577 0.001793 0.000770 0.001081 0.001474 0.001854
Nucleolus 0.000886 0.000677 0.000124 0.000271 0.000615 0.000467 0.000131 0.000496 0.000656 0.000635 0.000127 0.000465
Nucleus 0.093937 0.060286 0.403862* 0.123281 0.091495 0.059483 0.284783* 0.070196 0.091860 0.060128 0.353495* 0.077534
Peroxisomes 0.009643 0.003664 0.000087 0.000524 0.002473 0.008341 0.000446 0.004539 0.003544 0.004584 0.000239 0.003984
Vacuole 0.073661 0.066167 0.035467 0.253689* 0.056717 0.186586 0.069527 0.169379 0.059247 0.089856 0.049873 0.181033
Vacuole Periphery 0.000564 0.000758 0.000074 0.000233 0.000825 0.002575 0.000119 0.014978 0.000786 0.001116 0.000093 0.012940

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.06 -4.61 -3.84 -1.06 -1.28 -1.91 -4.31 -3.13 0.52 0.12 -3.93 -6.76 -3.44 -0.78 -0.71
Bud Neck 1.45 3.20 3.11 5.33 -0.66 -2.41 3.26 4.25 3.94 1.15 -1.50 5.44 5.23 6.20 0.33
Bud Site -5.35 1.15 -1.77 1.24 -2.38 -4.20 -2.32 -3.28 1.65 -0.23 -7.38 -1.07 -3.70 3.53 -2.76
Cell Periphery 4.79 4.42 3.13 -3.42 -2.61 0.94 3.41 7.27 1.72 5.48 5.56 5.56 7.79 1.66 4.02
Cytoplasm -10.29 0.27 4.67 16.72 6.07 5.61 8.71 9.56 -0.12 1.27 -8.24 9.19 10.42 19.03 3.16
Cytoplasmic Foci 10.97 18.21 17.63 21.33 -5.01 5.28 35.61 33.62 15.09 -9.90 16.47 39.88 37.66 25.53 -11.95
Eisosomes 3.99 4.38 3.41 -5.82 -10.96 2.74 15.40 13.09 4.85 -7.18 9.01 12.12 9.70 1.18 -14.30
Endoplasmic Reticulum 0.48 -4.64 -3.64 -5.82 0.37 1.25 -6.82 -1.18 -2.55 7.85 1.67 -8.99 -2.63 -5.55 8.79
Endosome 1.23 6.61 5.60 6.93 -5.92 -5.27 12.37 5.39 6.95 -4.35 -2.01 14.09 6.58 7.12 -4.81
Golgi -0.31 3.58 3.48 3.51 -4.54 -1.41 3.76 0.29 1.60 -3.43 0.04 4.16 0.55 0.55 -3.45
Lipid Particles 3.49 3.67 3.14 -3.14 -5.42 0.89 6.05 6.15 1.87 1.41 5.51 7.21 6.90 1.69 -4.04
Mitochondria 1.15 2.26 2.53 3.01 5.30 -1.99 2.12 -0.41 1.84 -2.53 -0.14 2.65 -0.24 -0.11 -2.80
Mitotic Spindle -4.29 -4.94 -4.19 -1.05 -2.32 -1.92 -4.55 -6.46 -1.23 -2.51 -4.07 -5.44 -7.18 -2.54 -4.09
None -1.21 -3.32 -2.22 -1.71 0.46 2.30 -2.78 -2.36 -6.82 -0.11 -0.34 -3.53 -2.75 -3.54 0.14
Nuclear Periphery 1.03 -19.60 -11.26 -11.93 -3.35 0.84 -0.89 -0.65 -14.10 2.01 1.05 -0.90 -1.01 -20.36 -0.99
Nuclear Periphery Foci -2.14 -4.62 -5.46 -4.37 -1.98 0.90 -5.47 -3.60 -6.90 3.24 -1.95 -6.52 -4.80 -3.51 2.70
Nucleolus 0.78 2.97 2.58 4.47 -3.12 1.24 4.08 2.97 2.87 -3.76 0.19 5.01 3.69 4.46 -4.84
Nucleus 3.43 -31.52 -3.57 -7.32 23.29 4.54 -32.55 -9.76 -11.36 23.43 7.57 -53.13 -10.38 -17.72 39.62
Peroxisomes 1.58 2.49 2.27 3.51 -1.19 -2.42 7.03 2.58 3.02 -2.14 -0.95 5.31 3.32 4.92 -2.62
Vacuole -0.24 0.80 -15.87 -16.88 -17.41 -11.30 -15.17 -31.43 -7.17 -16.88 -8.56 -10.50 -34.78 -27.31 -28.67
Vacuole Periphery -1.32 6.02 5.17 6.68 -2.49 -2.09 6.09 -2.69 -0.55 -3.72 -1.29 6.84 -2.60 -2.19 -3.74
ORF
Human Ortholog
Description Putative protein of unknown function; conserved across S. cerevisiae strains; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKL102C

YKL102C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl102c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available