Standard name
Human Ortholog
Description Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.13 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0.07 0.16 0.14 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0.38 0.22 0.15 0.12 0.05 0 0.06 0.06 0.07 0.11 0.11
Mitochondria 0.94 0.93 0.89 0.89 0.88 0.9 0.9 0.83 0.89 0.92 0.91 0.94 0.81 0.62 0.75 0.1 0 0.05 0.84 0.77 0.72 0.8 0.67 0.71
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0.06 0 0.07 0.1 0.1 0.08 0.08 0.09 0.08 0.11 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Nucleolus 0.05 0 0 0.05 0.07 0.1 0.11 0.14 0.11 0.08 0.08 0.07 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.17 0.1 0 0 0 0.06 0 0 0 0 0 0 0.1 0.22 0.24 0.23 0.06 0 0.13 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0.13 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.08 0 0 0.05 0 0 0 0 0 0 0 0.12 0.17 0.11 0.17 0.2 0.19 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 7 2 1 0 3 2 1 0 0 0 5 2 3 1 1 1 3 3 7 3 7 9
Bud 7 6 15 7 8 20 23 19 23 17 16 16 1 4 7 9 8 8 0 2 0 2 4 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 1 7 6 10 6 11 5 8 0 0 0 2 1 5 – – – – – –
Cell Periphery 3 4 9 3 1 1 2 2 4 4 2 6 7 13 18 15 16 9 0 0 0 0 0 0
Cytoplasm 8 2 5 9 11 11 12 15 7 12 12 6 3 8 10 20 12 15 0 0 0 0 2 3
Endoplasmic Reticulum 2 3 0 0 0 0 0 2 0 1 0 1 3 2 2 3 4 3 0 0 0 0 0 0
Endosome 1 2 15 5 10 11 2 9 1 0 1 2 14 26 32 11 29 22 4 13 13 5 16 9
Golgi 0 1 3 1 0 1 0 0 0 0 0 0 60 136 98 24 22 8 9 14 22 18 40 36
Mitochondria 234 219 313 163 260 492 460 456 280 601 339 548 204 222 332 16 8 8 199 191 257 219 256 229
Nucleus 0 2 2 0 1 7 4 6 0 6 2 1 0 1 1 3 6 14 0 0 0 0 2 0
Nuclear Periphery 15 9 21 6 22 52 49 45 26 62 31 65 10 7 5 7 11 13 1 1 1 1 3 2
Nucleolus 13 7 8 9 20 56 55 75 36 52 31 40 3 5 4 7 14 11 0 0 0 1 3 1
Peroxisomes 2 7 60 19 11 9 11 31 7 11 3 5 8 9 45 34 44 36 13 9 48 8 16 17
SpindlePole 1 3 13 0 6 6 7 31 14 14 4 11 2 11 12 10 24 23 0 0 1 1 2 0
Vac/Vac Membrane 25 20 9 3 15 13 13 13 7 23 7 14 30 61 49 27 36 30 3 7 0 10 20 8
Unique Cell Count 249 236 352 184 296 545 512 550 314 656 372 580 251 358 440 157 182 159 239 248 358 276 383 323
Labelled Cell Count 311 286 480 227 367 686 647 716 412 814 453 723 350 507 618 189 236 206 239 248 358 276 383 323


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 7.8 8.1 8.4 7.8 6.9 6.6 6.9 6.2 6.1 5.7 5.7 9.8 9.1 8.5 9.2 8.8 8.3 10.1 10.0 10.9
Std Deviation (1e-4) 1.4 1.7 1.9 2.3 2.0 1.8 1.7 2.0 2.1 1.6 1.6 1.5 1.8 1.5 1.4 2.3 2.2 1.9 2.1 2.1 2.0
Intensity Change (Log2) – – – 0.05 -0.05 -0.24 -0.29 -0.23 -0.38 -0.43 -0.51 -0.51 0.26 0.16 0.07 0.17 0.11 0.03 0.31 0.3 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.3 -1.1 -0.4 0.2 -0.6 1.7 -1.4 0 -1.2 -2.9 -2.6 -2.3 0.7 0.1 0.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 -1.2 0 -2.2 -1.8 0.2 0.8 1.2 3.4 3.2 1.8
Cytoplasm 0 1.9 0.7 1.0 1.3 0.8 0.5 1.6 0 0 0.8 0.9 5.5 3.2 4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.9 -0.6 -2.0 -4.0 -2.4 -3.3 -5.3 -3.7 -4.3 0.7 1.7 1.8 1.3 4.6 3.9
Golgi 0 0 0 0 0 0 0 0 0 9.1 12.5 9.0 6.7 5.8 0
Mitochondria -0.1 -0.4 0.7 0.4 -2.5 0.1 1.4 1.0 3.1 -2.6 -8.3 -4.8 -17.2 -18.9 -18.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.4 0.7 1.9 1.9 1.2 1.2 1.9 1.2 2.7 -1.1 -2.7 -3.8 -0.7 0 0.9
Nucleolus 1.6 2.8 4.5 4.7 5.8 4.8 3.6 3.6 3.1 0 -0.9 -1.6 0 3.0 2.6
Peroxisomes -2.1 -5.4 -8.4 -7.8 -5.5 -6.3 -9.1 -7.7 -9.4 -5.3 -6.5 -2.8 1.2 2.0 1.5
SpindlePole 0 -1.3 -2.6 -2.2 1.3 0.5 -1.5 -2.3 -1.7 -2.3 -0.5 -0.8 1.3 4.1 4.4
Vacuole 0 1.7 -0.2 0 -0.2 -0.3 0.8 -0.6 -0.1 4.6 6.5 4.6 6.0 6.8 6.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.314 11.1564 10.5338 9.3631 9.1178 10.2615 8.1622 9.9894 9.1427 8.7945 8.6064 8.9349 8.3992 9.8545 8.3583 9.323 7.6635 8.6845
Actin 0.0006 0.0027 0.0028 0.0006 0.0002 0.0017 0.0302 0.0018 0.0035 0.0003 0.0013 0.0055 0.0311 0.0033 0.0058 0.0019 0.0044 0.0032
Bud 0.0001 0.0001 0.0003 0.0001 0 0 0.0003 0.0001 0.0001 0 0.0004 0.0002 0.0004 0.0003 0.0004 0 0.0011 0
Bud Neck 0.0006 0.0001 0.0002 0 0 0 0.0012 0.0001 0.0002 0 0.0001 0.0001 0.0007 0.0001 0.0001 0 0.0002 0.0001
Bud Periphery 0.0002 0.0002 0.0004 0.0002 0.0001 0.0001 0.0004 0.0002 0.0002 0 0.0006 0.0004 0.0009 0.0007 0.0006 0.0001 0.0092 0.0001
Bud Site 0.0011 0.0002 0.0009 0.0001 0 0.0001 0.0023 0.0017 0.0007 0 0.0006 0.0001 0.0014 0.0003 0.0004 0 0.0008 0.0001
Cell Periphery 0.0004 0.0003 0.0002 0 0 0.0001 0.0003 0.0004 0.0001 0 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0007 0.0001
Cytoplasm 0 0.0001 0 0 0 0 0.0022 0 0.0001 0 0.0013 0.0001 0.0002 0 0.0002 0 0.0003 0
Cytoplasmic Foci 0.0007 0.0041 0.0008 0.0016 0 0.0004 0.0118 0.0026 0.0044 0.0003 0.0007 0.0047 0.0068 0.0018 0.0037 0.0006 0.0041 0.0017
Eisosomes 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0007 0.0003 0.0002 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0001 0.0001 0 0 0 0 0.0036 0 0.0002 0 0.0039 0.0001 0.0003 0.0001 0.0002 0 0.0017 0.0001
Endosome 0.0019 0.0178 0.0011 0.0019 0.0002 0.0012 0.027 0.0029 0.0078 0.0027 0.0168 0.0065 0.0256 0.0066 0.0116 0.001 0.0125 0.0049
Golgi 0.0025 0.0393 0.005 0.0031 0.0004 0.0045 0.0249 0.011 0.0099 0.0061 0.0059 0.0119 0.0366 0.0343 0.0272 0.0048 0.0129 0.0108
Lipid Particles 0.0043 0.0151 0.0043 0.0026 0.0002 0.0138 0.0234 0.0181 0.0114 0.0003 0.0005 0.0084 0.013 0.0087 0.0145 0.0072 0.0633 0.0044
Mitochondria 0.9545 0.8917 0.9635 0.9747 0.9979 0.9667 0.8275 0.9418 0.9468 0.9885 0.9592 0.9551 0.8481 0.9196 0.9225 0.9791 0.8252 0.9642
None 0 0 0 0 0 0 0.0009 0 0.0001 0 0.0001 0.0001 0.0002 0 0 0 0.0003 0
Nuclear Periphery 0.0001 0.0003 0.0035 0 0 0.0001 0.015 0.0002 0.0005 0 0.0022 0.0002 0.0004 0.0001 0.0004 0 0.0026 0.0002
Nucleolus 0.0214 0.0056 0.0003 0 0 0.003 0.0003 0.0012 0.0006 0 0 0 0.0004 0.0003 0.0001 0 0.0009 0.0001
Nucleus 0.0013 0.0003 0.0001 0 0 0.0005 0.0026 0.0002 0.0002 0 0.0004 0 0.0003 0.0001 0.0001 0 0.0009 0.0001
Peroxisomes 0.0055 0.0141 0.007 0.0142 0.0005 0.0054 0.0129 0.0113 0.0067 0.0009 0.0003 0.0017 0.0205 0.0089 0.0087 0.0047 0.0111 0.0051
Punctate Nuclear 0 0 0 0 0 0 0.0015 0 0.0006 0 0 0.0007 0.0002 0 0.0001 0 0.0024 0
Vacuole 0.0023 0.0022 0.0023 0.0002 0.0001 0.0005 0.0023 0.0029 0.0025 0.0001 0.0036 0.0005 0.0042 0.0057 0.001 0.0001 0.0193 0.0006
Vacuole Periphery 0.0023 0.0056 0.0071 0.0004 0.0003 0.0016 0.0091 0.0033 0.0034 0.0005 0.002 0.0034 0.0078 0.0087 0.002 0.0003 0.0257 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.1762 18.9128 33.466 35.4196 37.4533 28.4644 29.7873 29.2879 30.9648 40.9253
Translational Efficiency 0.8503 1.5594 0.8139 0.6623 0.7632 0.8747 0.8865 0.8625 0.7945 0.6806

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Aat1

Aat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available