Standard name
Human Ortholog
Description Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.08 0.06 0.09 0.1 0.11 0.1 0.08 0.09 0.11 0.15 0.07 0.12 0.16 0.14 0.05 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0 0 0.06 0.24 0.34 0.29 0.62 0.51 0.71 0.43 0 0 0 0 0 0 0 0 0.05 0 0.05 0.07
Nucleus 0.8 0.83 0.84 0.86 0.8 0.78 0.65 0.68 0.57 0.56 0.52 0.57 0.88 0.82 0.75 0.75 0.79 0.81 0.72 0.68 0.67 0.61 0.47 0.34
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.15 0.07 0 0.07 0 0.05 0 0.06 0.05 0.07 0.1 0 0 0.09 0.07 0.08 0 0.16 0.23 0.17 0.26 0.35 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 1 3 2 4 11 5 6 0 0 0 0 1 0 2 2 4 6 8 11
Bud Neck 0 0 0 0 0 0 0 1 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 1 5 3 18 8 12 5 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 4 14 20 17 35 37 53 50 42 43 45 68 25 48 84 29 6 21 1 2 6 9 10 11
Endoplasmic Reticulum 0 2 0 0 0 0 2 0 0 0 0 0 1 0 0 1 0 2 0 0 0 1 1 1
Endosome 0 2 0 3 10 0 0 4 0 0 0 2 0 4 6 3 1 0 3 5 1 2 8 16
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 4 0 1 0 1 0 4 1 3 1 4
Mitochondria 38 8 4 12 25 88 164 141 314 257 304 199 9 6 4 7 1 5 4 6 13 12 21 32
Nucleus 126 244 199 238 317 290 318 330 287 280 224 265 300 329 391 154 88 165 118 212 175 220 206 164
Nuclear Periphery 1 0 0 0 4 2 7 10 5 8 2 3 1 1 1 2 5 5 0 1 0 0 2 7
Nucleolus 0 2 0 1 1 2 12 8 6 8 10 8 2 5 5 9 6 9 0 0 0 4 20 25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 4 8 0 0 0 1 0 0 0 0 1
SpindlePole 0 2 1 0 1 0 1 4 1 5 0 1 3 1 2 1 1 1 4 2 5 6 4 18
Vac/Vac Membrane 7 45 17 12 26 14 24 21 30 27 28 46 5 18 48 14 9 8 26 72 44 93 155 182
Unique Cell Count 157 295 236 276 398 372 487 486 506 504 427 463 340 402 522 206 111 203 165 314 263 365 443 489
Labelled Cell Count 176 319 241 283 420 435 589 575 708 650 630 603 348 421 549 222 118 218 165 314 263 365 443 489


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 7.6 7.3 6.6 6.7 5.5 5.3 5.8 4.5 4.6 4.1 4.7 7.5 7.9 7.7 11.2 12.0 11.9 7.2 7.5 8.0
Std Deviation (1e-4) 1.5 1.9 1.9 1.7 2.1 1.9 2.0 2.3 1.5 1.3 1.5 1.8 2.1 2.5 2.3 3.8 3.1 3.4 1.9 2.1 2.4
Intensity Change (Log2) -0.15 -0.11 -0.41 -0.46 -0.32 -0.71 -0.67 -0.84 -0.64 0.05 0.13 0.09 0.63 0.72 0.72 -0.02 0.05 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 0.1 0.6 1.0 0.8 -0.1 0 0.9 2.3 -0.5 1.4 2.8 1.9 -1.0 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 2.7 7.4 9.5 8.6 0 0 0 0 0.8 0 0 1.1 0 0
Nucleus 0.6 -1.5 -1.9 -5.3 -4.7 -7.4 -7.6 -8.2 -7.2 1.4 -0.8 -2.9 -2.5 -1.2 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 -0.3 -1.9 -1.2 -1.6 -0.7 -1.0 -0.3 1.2 -3.5 -1.5 0.9 -0.2 0.3 -1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1594 4.9702 4.5924 4.5728 4.8628 4.6259 4.1337 4.3702 4.0355 3.247 3.3607 3.7232 4.1644 4.065 3.616 3.873 2.7159 3.4863
Actin 0.0218 0 0.0019 0.0218 0.0151 0.0004 0.0031 0 0.0001 0.0001 0.0007 0 0.0168 0 0.0074 0.0273 0.0128 0.0061
Bud 0.0006 0 0.0002 0.0008 0.0004 0.0001 0.0001 0 0 0.0003 0.0001 0 0.0015 0 0.0003 0.0006 0.0007 0.0003
Bud Neck 0.0013 0.0002 0.002 0.0043 0.0018 0.001 0.0002 0 0 0.0008 0.0004 0.0009 0.0053 0.0002 0.0009 0.001 0.0012 0.0039
Bud Periphery 0.0013 0 0.0004 0.0027 0.001 0.0003 0.0001 0 0 0.0006 0.0002 0.0001 0.0036 0.0001 0.0005 0.0011 0.001 0.0011
Bud Site 0.0021 0.0001 0.0008 0.0019 0.0014 0.0001 0.0002 0 0 0.0004 0.0006 0.0001 0.0067 0.0002 0.0021 0.0031 0.0028 0.0002
Cell Periphery 0.0003 0 0.0002 0.0007 0.0004 0.0001 0.0001 0 0 0.0003 0 0 0.0008 0 0.0002 0.0003 0.0001 0.0002
Cytoplasm 0.0029 0.0002 0.0013 0.0005 0.0137 0.001 0.0013 0.0001 0 0.01 0.0002 0.0001 0.0072 0.0002 0.0021 0.0068 0.0013 0.0011
Cytoplasmic Foci 0.0095 0 0.0031 0.0011 0.005 0.0003 0.0061 0 0.0004 0.0004 0.0036 0 0.0088 0 0.0028 0.0094 0.0193 0.0002
Eisosomes 0.0004 0 0 0.0015 0.0002 0 0.0001 0 0 0 0 0 0.0002 0 0.0002 0.0003 0.0001 0
Endoplasmic Reticulum 0.0029 0 0.0008 0.0032 0.0066 0.0011 0.0018 0 0 0.0007 0.0001 0 0.0036 0.0001 0.0012 0.0006 0.0009 0.0005
Endosome 0.0103 0.0001 0.0042 0.0094 0.0202 0.0023 0.0059 0 0 0.0008 0.0031 0 0.0223 0.0001 0.0059 0.0119 0.016 0.0014
Golgi 0.0033 0 0.0014 0.0051 0.0047 0.0001 0.0016 0 0.0001 0 0.0023 0 0.0055 0 0.0021 0.0074 0.0078 0.0006
Lipid Particles 0.0097 0 0.0076 0.0027 0.0101 0.0003 0.0112 0 0.0004 0.0003 0.0281 0 0.0144 0 0.0096 0.0357 0.0167 0.0002
Mitochondria 0.0082 0.0002 0.0057 0.0369 0.0292 0.0019 0.0028 0.0001 0.0002 0.0005 0.0028 0.0001 0.0145 0.0007 0.006 0.0057 0.0042 0.008
None 0.0097 0 0.0013 0.0009 0.0046 0.0011 0.0027 0 0 0.0152 0.0003 0 0.0052 0.0105 0.0004 0.0357 0.0018 0.0003
Nuclear Periphery 0.0201 0.0028 0.0111 0.0052 0.0492 0.0097 0.004 0.0025 0.0065 0.006 0.0024 0.0015 0.0453 0.006 0.0105 0.0027 0.0104 0.0063
Nucleolus 0.0246 0.028 0.0252 0.0228 0.0903 0.0641 0.0138 0.0207 0.0167 0.06 0.0714 0.0519 0.0367 0.0274 0.0234 0.0165 0.0493 0.061
Nucleus 0.838 0.964 0.9156 0.8678 0.7151 0.9042 0.9226 0.9743 0.9684 0.8737 0.8563 0.9402 0.7638 0.9524 0.9148 0.8083 0.7836 0.9038
Peroxisomes 0.0069 0 0.0027 0.0015 0.0041 0.0001 0.0099 0 0.0059 0.0001 0.0088 0 0.0055 0 0.0022 0.0061 0.0149 0.0001
Punctate Nuclear 0.0218 0.0041 0.0112 0.003 0.0191 0.0092 0.0112 0.0021 0.0009 0.0245 0.0179 0.0047 0.0122 0.0008 0.0062 0.0171 0.0536 0.0015
Vacuole 0.0022 0.0002 0.0018 0.0035 0.0035 0.0017 0.0005 0.0001 0 0.0051 0.0004 0.0002 0.0142 0.0004 0.0008 0.0019 0.0008 0.0015
Vacuole Periphery 0.002 0.0001 0.0014 0.003 0.0044 0.0008 0.0005 0.0001 0.0001 0.0004 0.0002 0 0.006 0.0007 0.0005 0.0006 0.0007 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.4846 34.8448 30.4972 36.3212 33.692 37.9585 32.9731 22.5595 27.6892 38.9489
Translational Efficiency 2.4934 1.3925 1.7069 1.4064 1.8597 1.6934 1.5871 2.0298 1.6773 1.565

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Apn1

Apn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available