Standard name | |
---|---|
Human Ortholog | |
Description | Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 2 | 3 | 0 | 1 | 1 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | 1 | 3 | 0 | 0 | 1 | 2 | 2 | 3 | 2 | 1 | 5 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 190 | 147 | 67 | 110 | 130 | 101 | 83 | 76 | 63 | 35 | 57 | 60 | 131 | 126 | – | – | – | 27 | 211 | 129 | 51 | 70 | 61 |
Endoplasmic Reticulum | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 2 | 0 | 1 | 1 | 0 | – | – | – | 0 | 0 | 2 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 0 | 0 | 0 | 1 |
Golgi | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | – | 3 | 7 | 12 | 30 | 59 | 64 | 61 | 77 | 75 | 55 | 63 | 1 | 3 | 5 | – | – | – | 0 | 0 | 2 | 0 | 1 | 2 |
Nucleus | – | 3 | 4 | 0 | 2 | 1 | 0 | 3 | 2 | 3 | 3 | 5 | 0 | 0 | 1 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 8 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | – | 5 | 1 | 1 | 6 | 7 | 10 | 12 | 11 | 10 | 3 | 5 | 0 | 3 | 0 | – | – | – | 0 | 2 | 0 | 0 | 0 | 2 |
Unique Cell Count | 205 | 154 | 71 | 131 | 159 | 148 | 129 | 132 | 121 | 81 | 111 | 62 | 134 | 128 | 31 | 225 | 144 | 53 | 77 | 75 | ||||
Labelled Cell Count | 214 | 163 | 80 | 150 | 199 | 180 | 162 | 175 | 159 | 103 | 139 | 62 | 139 | 133 | 31 | 225 | 144 | 53 | 77 | 75 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 6.5 | 5.8 | 5.1 | 5.3 | 5.1 | 4.8 | 4.9 | 4.9 | 4.6 | 4.6 | 4.5 | 5.8 | 5.6 | 5.5 | – | – | – | 4.8 | 5.6 | 6.4 |
Std Deviation (1e-4) | – | 1.1 | 1.4 | 1.1 | 1.6 | 1.5 | 1.8 | 1.9 | 1.5 | 1.8 | 1.3 | 1.0 | 1.7 | 1.6 | 1.4 | – | – | – | 0.5 | 2.2 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.1 | -0.18 | -0.26 | -0.22 | -0.23 | -0.32 | -0.31 | -0.35 | 0.02 | -0.03 | -0.06 | – | – | – | -0.26 | -0.05 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | -0.4 | -3.2 | -3.8 | -6.2 | -6.7 | -7.7 | -8.4 | -9.1 | -8.4 | 0.4 | 1.1 | 1.4 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 3.1 | 4.6 | 7.1 | 0 | 8.4 | 10.0 | 0 | 0 | 0 | 0 | 0 | -0.3 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 2.8 | 3.5 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5908 | 10.7697 | 17.3934 | 37.8507 | 14.7012 | 13.4573 | 10.4088 | 12.9697 | 26.4033 | 21.438 |
Translational Efficiency | 0.6842 | 0.7438 | 0.805 | 0.8117 | 0.9656 | 0.9246 | 0.8415 | 0.6376 | 0.844 | 0.826 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
325 | 126 | 1514 | 1 | 71 | 165 | 235 | 28 | 396 | 291 | 1749 | 29 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 763.61 | 745.11 | 849.41 | 255.99 | 728.62 | 814.79 | 839.50 | 362.53 | 757.34 | 784.62 | 848.08 | 358.86 |
Standard Deviation | 77.19 | 98.44 | 97.64 | — | 120.90 | 110.91 | 75.26 | 20.80 | 87.70 | 111.19 | 95.00 | 28.21 |
Intensity Change Log 2 | — | -0.035383 | 0.153625 | -1.576749 | — | 0.161262 | 0.204364 | -1.007066 | — | 0.063984 | 0.178623 | -1.270578 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000128 | 0.000195 | 0.000098 | 0.000141 | 0.000247 | 0.000161 | 0.000072 | 0.000167 | 0.000149 | 0.000176 | 0.000095 | 0.000166 |
Bud Neck | 0.001708 | 0.036373 | 0.004194 | 0.000004 | 0.004307 | 0.003102 | 0.003349 | 0.000009 | 0.002174 | 0.017508 | 0.004081 | 0.000008 |
Bud Site | 0.002726 | 0.053485 | 0.010311 | 0.000016 | 0.024309 | 0.005114 | 0.008009 | 0.000038 | 0.006595 | 0.026058 | 0.010002 | 0.000037 |
Cell Periphery | 0.000152 | 0.000093 | 0.000094 | 0.000139 | 0.000171 | 0.000103 | 0.000112 | 0.000810 | 0.000155 | 0.000099 | 0.000097 | 0.000786 |
Cytoplasm | 0.653362* | 0.518617* | 0.734000* | 0.000019 | 0.488058* | 0.643026* | 0.594699* | 0.000174 | 0.623724* | 0.589158* | 0.715283* | 0.000168 |
Cytoplasmic Foci | 0.157327 | 0.113713 | 0.023273 | 0.000015 | 0.153601 | 0.109060 | 0.043401 | 0.000019 | 0.156659 | 0.111075 | 0.025977 | 0.000019 |
Eisosomes | 0.000085 | 0.000058 | 0.000022 | 0.000874 | 0.000075 | 0.000056 | 0.000043 | 0.001204 | 0.000083 | 0.000057 | 0.000025 | 0.001193 |
Endoplasmic Reticulum | 0.000425 | 0.000355 | 0.000912 | 0.000000 | 0.000726 | 0.000676 | 0.000773 | 0.000006 | 0.000479 | 0.000537 | 0.000893 | 0.000006 |
Endosome | 0.008846 | 0.019375 | 0.002588 | 0.000000 | 0.025624 | 0.009286 | 0.005636 | 0.000000 | 0.011854 | 0.013655 | 0.002998 | 0.000000 |
Golgi | 0.001720 | 0.001612 | 0.000120 | 0.000003 | 0.001450 | 0.002253 | 0.000339 | 0.000006 | 0.001671 | 0.001976 | 0.000150 | 0.000006 |
Lipid Particles | 0.002282 | 0.003118 | 0.000741 | 0.000017 | 0.017808 | 0.003146 | 0.001056 | 0.000084 | 0.005066 | 0.003134 | 0.000783 | 0.000082 |
Mitochondria | 0.000520 | 0.000257 | 0.000263 | 0.000005 | 0.000832 | 0.006665 | 0.000592 | 0.000032 | 0.000576 | 0.003890 | 0.000307 | 0.000031 |
Mitotic Spindle | 0.000790 | 0.002255 | 0.002093 | 0.000197 | 0.043186 | 0.000548 | 0.004770 | 0.000321 | 0.008391 | 0.001287 | 0.002452 | 0.000316 |
None | 0.001638 | 0.010046 | 0.004036 | 0.998337* | 0.001555 | 0.001896 | 0.003819 | 0.996169* | 0.001623 | 0.005425 | 0.004007 | 0.996244* |
Nuclear Periphery | 0.000227 | 0.000141 | 0.000473 | 0.000000 | 0.000249 | 0.000332 | 0.000573 | 0.000000 | 0.000231 | 0.000249 | 0.000487 | 0.000000 |
Nuclear Periphery Foci | 0.001012 | 0.000814 | 0.001399 | 0.000001 | 0.001271 | 0.000963 | 0.000998 | 0.000001 | 0.001059 | 0.000899 | 0.001345 | 0.000001 |
Nucleolus | 0.000371 | 0.001009 | 0.000294 | 0.000001 | 0.000346 | 0.000390 | 0.000497 | 0.000002 | 0.000366 | 0.000658 | 0.000321 | 0.000002 |
Nucleus | 0.077495 | 0.030237 | 0.170106* | 0.000004 | 0.035404 | 0.087652 | 0.222382* | 0.000013 | 0.069948 | 0.062792 | 0.177130* | 0.000013 |
Peroxisomes | 0.001796 | 0.005624 | 0.000610 | 0.000228 | 0.008091 | 0.007101 | 0.000299 | 0.000942 | 0.002925 | 0.006462 | 0.000568 | 0.000918 |
Vacuole | 0.087072 | 0.201486* | 0.044086 | 0.000000 | 0.191292 | 0.117241 | 0.107008 | 0.000000 | 0.105758 | 0.153718 | 0.052541 | 0.000000 |
Vacuole Periphery | 0.000320 | 0.001134 | 0.000286 | 0.000000 | 0.001398 | 0.001230 | 0.001573 | 0.000000 | 0.000513 | 0.001189 | 0.000459 | 0.000000 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | NaN | NaN | NaN | NaN | NaN | 0.57 | 2.09 | 0.74 | 0.19 | -2.15 | -0.62 | 0.62 | -0.30 | 0.36 | -1.30 |
Bud Neck | NaN | NaN | NaN | NaN | NaN | 1.01 | 0.91 | 4.48 | 4.44 | 2.42 | -3.35 | -1.99 | 10.67 | 3.86 | 4.72 |
Bud Site | NaN | NaN | NaN | NaN | NaN | 1.83 | 1.59 | 2.37 | 4.20 | 2.50 | -3.70 | -1.33 | 3.63 | 5.44 | 6.23 |
Cell Periphery | NaN | NaN | NaN | NaN | NaN | 1.53 | 1.57 | -2.71 | -2.97 | -2.97 | 3.90 | 4.37 | -2.73 | -2.98 | -2.97 |
Cytoplasm | NaN | NaN | NaN | NaN | NaN | -3.37 | -2.09 | 12.33 | 28.32 | 32.36* | 2.33 | -4.89 | 49.65 | 32.90 | 115.77* |
Cytoplasmic Foci | NaN | NaN | NaN | NaN | NaN | 2.16 | 5.63 | 7.73 | 10.95 | 10.21 | 4.89 | 18.83 | 23.05 | 13.73 | 22.17 |
Eisosomes | NaN | NaN | NaN | NaN | NaN | 1.31 | 2.47 | -4.61 | -4.68 | -4.72 | 4.07 | 11.12 | -4.68 | -4.79 | -4.91 |
Endoplasmic Reticulum | NaN | NaN | NaN | NaN | NaN | -0.46 | -1.05 | 3.13 | 4.60 | 8.35 | -1.22 | -7.54 | 7.87 | 5.71 | 17.65 |
Endosome | NaN | NaN | NaN | NaN | NaN | 3.52 | 4.53 | 5.87 | 4.34 | 3.82 | -0.41 | 8.00 | 11.11 | 6.77 | 12.55 |
Golgi | NaN | NaN | NaN | NaN | NaN | -0.30 | 1.83 | 2.33 | 1.19 | 1.95 | -0.01 | 1.64 | 1.80 | 1.83 | 5.67 |
Lipid Particles | NaN | NaN | NaN | NaN | NaN | 0.95 | 1.09 | 1.16 | 2.06 | 6.69 | 0.72 | 1.58 | 1.90 | 2.61 | 11.65 |
Mitochondria | NaN | NaN | NaN | NaN | NaN | -0.89 | 0.54 | 1.37 | 1.04 | 1.35 | -0.88 | 0.92 | 1.86 | 1.07 | 3.80 |
Mitotic Spindle | NaN | NaN | NaN | NaN | NaN | 1.85 | 1.49 | 1.86 | 0.84 | 1.53 | 1.68 | 1.25 | 1.91 | 1.57 | 2.75 |
None | NaN | NaN | NaN | NaN | NaN | -0.73 | -2.39 | -911.13* | -960.04* | -980.50* | -1.05 | -5.05 | -1078.54* | -309.62* | -1125.10* |
Nuclear Periphery | NaN | NaN | NaN | NaN | NaN | -2.55 | -6.80 | 6.56 | 8.40 | 14.46 | -2.14 | -11.63 | 10.26 | 9.57 | 28.50 |
Nuclear Periphery Foci | NaN | NaN | NaN | NaN | NaN | -0.40 | -0.03 | 2.41 | 2.06 | 7.79 | -0.41 | -3.22 | 8.24 | 2.89 | 14.42 |
Nucleolus | NaN | NaN | NaN | NaN | NaN | -0.67 | -1.05 | 4.86 | 3.26 | 5.89 | -1.41 | 0.90 | 7.32 | 3.29 | 8.37 |
Nucleus | NaN | NaN | NaN | NaN | NaN | -5.53 | -12.55 | 5.33 | 10.32 | 16.99 | -0.01 | -18.87 | 16.25 | 11.26 | 42.70 |
Peroxisomes | NaN | NaN | NaN | NaN | NaN | 0.44 | 1.78 | 1.62 | 1.18 | -2.43 | -0.95 | 2.09 | 1.83 | 1.92 | -0.74 |
Vacuole | NaN | NaN | NaN | NaN | NaN | 2.44 | 1.92 | 7.59 | 10.68 | 11.94 | -4.56 | 4.65 | 14.71 | 15.02 | 23.04 |
Vacuole Periphery | NaN | NaN | NaN | NaN | NaN | 0.31 | 0.20 | 2.27 | 1.52 | 1.42 | -1.19 | 0.61 | 4.24 | 2.40 | 3.04 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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