Standard name
Human Ortholog
Description Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 1.0 0.93 0.96 0.91 0.84 0.8 0.75 0.81 0.84 0.81 0.99 1.0 0.99 0.98 0.97 0.98 0.97 0.96 0.95 0.97 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.1 0.08 0.15 0.1 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.1 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 3 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 5 1 4 1 4 8 1 5 0 3 0 3 0 0 1 0 0 0 0 0 0 0
Cytoplasm 162 286 177 113 151 52 129 148 39 54 53 77 327 430 405 44 161 150 151 274 172 317 345 420
Endoplasmic Reticulum 2 3 0 3 3 4 16 15 8 7 1 7 1 2 2 2 3 4 0 0 1 0 5 2
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0
Golgi 0 1 0 1 0 0 1 1 0 0 2 4 0 0 0 0 1 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 1 0 1 0 0 4 0 1 0 0 0 0 1 0 0 0 0 1 1
Nucleus 1 0 0 0 0 2 0 2 0 1 0 1 1 0 0 0 0 1 0 3 1 2 2 14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 0 4 3 1 6 12 5 3 4 5 0 2 4 0 6 2 0 1 0 1 5 10
Unique Cell Count 165 291 177 121 157 57 153 186 52 67 63 95 330 432 409 45 166 153 157 286 181 326 372 474
Labelled Cell Count 168 294 182 122 161 61 157 190 53 72 65 98 330 437 411 46 173 160 157 286 181 326 372 474


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 57.3 59.0 54.9 49.7 56.8 35.3 49.0 45.6 40.7 39.7 33.1 37.2 56.9 67.0 69.8 60.8 66.6 69.8 44.9 50.6 55.6
Std Deviation (1e-4) 14.7 13.3 9.8 12.2 14.2 13.8 15.8 13.1 12.0 15.5 14.6 12.9 12.6 17.8 19.4 29.8 28.0 25.2 11.1 14.2 16.8
Intensity Change (Log2) – – – -0.14 0.05 -0.64 -0.16 -0.27 -0.43 -0.47 -0.73 -0.56 0.05 0.29 0.35 0.15 0.28 0.35 -0.29 -0.12 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.8 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.5 -2.6 -4.0 -5.5 -6.4 -6.8 -6.0 -5.4 -6.0 -1.3 -0.9 -1.3 -2.0 -2.3 -1.9
Endoplasmic Reticulum 0 0 0 4.4 3.9 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.4 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 40.6082 66.1 55.4646 49.5094 49.6573 53.8977 50.064 70.4631 62.1676 60.0146 49.6612 63.1005 66.3155 81.0152 73.8168 70.6415 59.9685 73.1771
Actin 0.0207 0.0059 0.0134 0.0144 0.0013 0.0008 0.0173 0.0001 0.0052 0.0007 0.0008 0.0002 0.0071 0.0001 0.0003 0.0001 0.0213 0.0002
Bud 0.0029 0.0001 0.0008 0.0017 0.0006 0.0002 0.0012 0 0.0005 0.0001 0.0016 0.0001 0.0006 0 0.0001 0.0002 0.0003 0.0001
Bud Neck 0.0035 0.0007 0.0003 0.0019 0.0004 0.0009 0.0008 0.0001 0.0003 0.0002 0.0008 0.0005 0.0002 0.0001 0.0001 0.0001 0.0005 0.0003
Bud Periphery 0.0018 0 0.0006 0.0018 0.0004 0.0001 0.0008 0 0.0003 0 0.0008 0.0001 0.0005 0 0 0.0002 0.0005 0
Bud Site 0.0149 0.0002 0.0007 0.0087 0.0014 0 0.0073 0.0001 0.0004 0.0001 0.0005 0 0.001 0 0.0001 0.0001 0.0007 0
Cell Periphery 0.0005 0 0.0003 0.0005 0.0008 0.0001 0.0007 0.002 0.0001 0.0001 0.0006 0.0001 0.0005 0 0.0001 0.0003 0.0003 0
Cytoplasm 0.7741 0.9816 0.9205 0.8777 0.8607 0.9622 0.8183 0.9756 0.9194 0.9308 0.8324 0.9136 0.8795 0.9901 0.9591 0.9415 0.8203 0.9551
Cytoplasmic Foci 0.0211 0.0002 0.0066 0.0035 0.0122 0.0024 0.0138 0.0001 0.011 0.006 0.0174 0.0034 0.0169 0.0001 0.0028 0.0005 0.0062 0.0002
Eisosomes 0.0004 0.0001 0.0002 0.0002 0.0003 0 0.0002 0 0.0001 0.0003 0.0002 0 0.0006 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0064 0.0002 0.0006 0.0013 0.0018 0.0016 0.0042 0.0003 0.0007 0.0004 0.0037 0.0006 0.0032 0.0001 0.0005 0.0002 0.0085 0.0002
Endosome 0.0338 0.0007 0.0033 0.0068 0.0265 0.0007 0.007 0 0.0034 0.0004 0.0265 0.0031 0.0048 0 0.0004 0.001 0.0263 0.0001
Golgi 0.0056 0.0007 0.0051 0.0023 0.0093 0.0001 0.002 0 0.0012 0.0003 0.0049 0.0043 0.0013 0 0.0001 0 0.0109 0
Lipid Particles 0.0083 0.0001 0.0025 0.0009 0.0115 0.0002 0.0078 0.0002 0.0058 0.0065 0.0091 0.0005 0.0092 0 0.0008 0.0003 0.006 0
Mitochondria 0.0068 0.0002 0.0164 0.0047 0.01 0.0003 0.0066 0 0.0039 0.0046 0.0057 0.0006 0.0081 0 0.0064 0.0006 0.0074 0.0001
None 0.0088 0.0001 0.0033 0.0008 0.009 0.0011 0.0042 0.0001 0.0032 0.0034 0.0162 0.0003 0.0085 0.0001 0.002 0.0006 0.0044 0.0001
Nuclear Periphery 0.0171 0.001 0.0034 0.0073 0.0055 0.0035 0.0145 0.0019 0.0037 0.0027 0.0119 0.0056 0.0062 0.0007 0.0023 0.003 0.0206 0.0049
Nucleolus 0.0017 0 0.0023 0.0006 0.0104 0 0.0023 0.0001 0.001 0.0009 0.007 0.0008 0.0052 0 0.0005 0.0015 0.003 0
Nucleus 0.0276 0.0078 0.014 0.0526 0.0189 0.0229 0.0703 0.0183 0.0303 0.0402 0.0396 0.0587 0.0269 0.0088 0.0211 0.0316 0.0306 0.0378
Peroxisomes 0.0122 0.0001 0.0012 0.0005 0.0012 0 0.0057 0 0.0035 0.0008 0.001 0.0001 0.0091 0 0.0001 0 0.0006 0
Punctate Nuclear 0.0175 0.0002 0.0016 0.0006 0.0045 0.0003 0.0048 0.0001 0.0015 0.0005 0.0044 0.0004 0.0054 0 0.0013 0.0001 0.0043 0.0005
Vacuole 0.0102 0.0001 0.0018 0.0086 0.0079 0.0021 0.0075 0.0012 0.0028 0.0004 0.0095 0.0057 0.0032 0 0.0007 0.0111 0.0079 0.0002
Vacuole Periphery 0.0039 0 0.0012 0.0024 0.0056 0.0004 0.0026 0 0.0017 0.0005 0.0055 0.0014 0.0021 0 0.0013 0.0068 0.0194 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 151.6969 171.2143 200.1293 243.3358 224.1512 201.5859 208.7333 259.5311 282.2806 209.8693
Translational Efficiency 6.5399 5.624 4.6306 4.9884 4.0475 4.68 4.4728 3.9577 3.2488 3.6672

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
337 1592 216 868 1205 1834 173 2019 1542 3426 389 2887

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4561.28 3767.51 5643.38 6618.43 2991.33 3619.87 6352.48 6841.00 3334.44 3688.48 5958.74 6774.08
Standard Deviation 1270.77 747.30 1021.53 1386.30 706.56 721.10 1033.97 1573.87 1078.88 737.07 1085.84 1523.34
Intensity Change Log 2 — -0.275827 0.307121 0.537050 — 0.275151 1.086533 1.193420 — -0.031913 0.667489 0.833570

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000494 0.000063 0.000216 0.000114 0.000458 0.000066 0.000116 0.000113 0.000466 0.000065 0.000172 0.000113
Bud Neck 0.006017 0.000446 0.003670 0.001067 0.000934 0.000311 0.002058 0.000859 0.002045 0.000374 0.002953 0.000921
Bud Site 0.008859 0.000722 0.001344 0.000757 0.001990 0.001095 0.000734 0.001685 0.003491 0.000922 0.001073 0.001406
Cell Periphery 0.002895 0.000305 0.006009 0.000490 0.000244 0.000098 0.001122 0.000199 0.000823 0.000194 0.003836 0.000287
Cytoplasm 0.370889* 0.679182* 0.527419* 0.372183* 0.653890* 0.720517* 0.403094* 0.568879* 0.592041* 0.701310* 0.472128* 0.509741*
Cytoplasmic Foci 0.016521 0.003454 0.031986 0.004844 0.004918 0.002688 0.020987 0.002755 0.007454 0.003044 0.027094 0.003383
Eisosomes 0.000006 0.000001 0.000006 0.000002 0.000002 0.000000 0.000003 0.000001 0.000003 0.000001 0.000005 0.000001
Endoplasmic Reticulum 0.000272 0.000030 0.000039 0.001267 0.000132 0.000035 0.000025 0.000018 0.000162 0.000033 0.000033 0.000393
Endosome 0.000233 0.000034 0.000122 0.000116 0.000334 0.000084 0.000038 0.000110 0.000312 0.000061 0.000084 0.000112
Golgi 0.002253 0.000410 0.002269 0.000125 0.001898 0.000379 0.000345 0.000163 0.001976 0.000393 0.001414 0.000151
Lipid Particles 0.001818 0.000241 0.005226 0.000272 0.000415 0.000123 0.000775 0.000134 0.000722 0.000178 0.003246 0.000175
Mitochondria 0.001513 0.000150 0.000259 0.000318 0.000272 0.000117 0.000251 0.000252 0.000543 0.000133 0.000255 0.000272
Mitotic Spindle 0.001980 0.000514 0.000262 0.000351 0.002198 0.001427 0.000281 0.003749 0.002150 0.001003 0.000270 0.002727
None 0.009292 0.024283 0.006011 0.013118 0.011103 0.023418 0.006706 0.021097 0.010708 0.023820 0.006320 0.018698
Nuclear Periphery 0.000444 0.000086 0.001066 0.000317 0.000248 0.000054 0.000590 0.000136 0.000291 0.000069 0.000855 0.000190
Nuclear Periphery Foci 0.001990 0.000763 0.003084 0.001461 0.002003 0.000512 0.000831 0.000195 0.002000 0.000629 0.002082 0.000575
Nucleolus 0.000341 0.000451 0.000121 0.000109 0.000173 0.000076 0.000081 0.000082 0.000210 0.000250 0.000104 0.000090
Nucleus 0.565228* 0.286910* 0.381679* 0.598617* 0.312208* 0.246788* 0.547434* 0.396678* 0.367505* 0.265432* 0.455395* 0.457393*
Peroxisomes 0.000024 0.000010 0.000008 0.000005 0.000207 0.000021 0.000007 0.000019 0.000167 0.000016 0.000007 0.000015
Vacuole 0.006860 0.001801 0.028513 0.004302 0.005223 0.001925 0.014443 0.002829 0.005581 0.001867 0.022255 0.003272
Vacuole Periphery 0.002071 0.000142 0.000692 0.000165 0.001151 0.000265 0.000079 0.000048 0.001352 0.000208 0.000419 0.000083

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.19 2.04 4.04 -6.84 4.92 1.61 0.96 1.53 -0.56 3.64 2.14 0.97 1.90 -1.92 6.07
Bud Neck 6.91 0.89 5.35 -7.63 5.10 6.84 -5.94 -0.47 -6.02 5.63 8.77 -5.05 3.68 -8.33 7.41
Bud Site 3.84 3.15 3.56 -3.02 3.02 1.66 0.56 0.79 -1.73 0.54 3.55 2.15 2.54 -2.58 1.23
Cell Periphery 6.02 -4.71 4.95 -7.17 8.56 5.57 -8.62 0.73 -6.18 8.87 6.39 -8.54 4.33 -7.34 9.85
Cytoplasm -23.04 -4.99 -0.01 36.16 5.97 -10.37 17.42 11.27 26.16 -12.00 -16.74 13.23 11.18 38.12 -6.63
Cytoplasmic Foci 7.48 -6.79 4.01 -6.26 9.02 3.90 -7.46 1.97 -2.96 7.76 6.93 -11.46 3.23 -5.75 12.31
Eisosomes 7.53 -2.06 2.40 -7.55 4.49 3.27 -5.91 0.32 -5.87 6.57 6.65 -7.43 0.94 -9.46 8.58
Endoplasmic Reticulum 5.40 4.93 -0.49 -1.04 -0.99 5.04 4.56 5.78 1.62 3.67 7.17 6.36 0.08 -0.98 -0.86
Endosome 3.47 0.95 2.89 -0.42 2.73 2.70 2.79 2.80 0.33 0.34 3.44 2.47 3.32 -0.10 2.29
Golgi 3.45 -0.35 4.21 6.12 6.24 2.92 2.63 3.59 2.36 5.37 3.75 0.75 4.62 4.45 6.91
Lipid Particles 2.20 -2.42 1.71 -5.26 4.48 5.03 -4.77 1.61 -4.19 5.61 3.51 -4.48 2.06 -5.59 5.38
Mitochondria 7.81 6.90 6.78 -8.95 -0.92 5.66 -0.23 0.84 -5.37 1.18 9.07 5.17 5.60 -7.52 0.84
Mitotic Spindle 1.23 2.26 1.20 -0.11 -1.87 1.45 3.58 -1.62 -2.94 -4.73 2.16 4.15 -1.11 -3.22 -5.03
None -5.95 1.26 -1.05 7.76 -4.95 -9.56 5.19 -7.19 3.30 -12.58 -11.97 5.40 -6.13 6.72 -14.13
Nuclear Periphery 7.77 -5.94 0.26 -10.52 6.15 9.29 -8.86 -0.88 -13.09 8.68 11.19 -9.55 -1.07 -16.10 9.35
Nuclear Periphery Foci 1.94 -1.53 1.62 -0.15 4.04 4.85 2.30 6.11 1.34 5.69 5.12 -0.90 5.53 1.13 6.39
Nucleolus -0.31 2.25 2.82 0.96 2.25 4.23 2.03 2.24 -3.37 0.42 -0.25 2.88 3.71 0.79 1.77
Nucleus 18.99 9.07 -1.15 -32.69 -12.82 9.37 -11.16 -10.53 -23.79 6.47 14.96 -6.35 -11.01 -34.88 -0.68
Peroxisomes 1.67 2.23 4.06 0.86 4.29 1.15 1.20 1.23 1.00 0.70 1.21 1.24 1.27 1.00 1.02
Vacuole 4.92 -6.74 -4.36 -10.54 4.82 6.59 -7.08 -3.47 -10.53 6.08 8.29 -9.84 -5.48 -14.85 8.02
Vacuole Periphery 3.62 2.30 3.75 0.84 4.32 1.48 1.81 2.00 1.29 5.59 2.44 1.83 2.87 1.59 5.01
Standard name
Human Ortholog
Description Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sba1

Sba1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sba1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available