Standard name
Human Ortholog
Description Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0.11 0 0 0 0 0 0
Cytoplasm 0.59 0.66 0.69 0.73 0.79 0.72 0.65 0.61 0.63 0.62 0.57 0.59 0.72 0.82 0.82 0.87 0.86 0.82 0.11 0.24 0.17 0.16 0.23 0.26
Endoplasmic Reticulum 0.31 0.32 0.19 0.07 0 0 0 0 0 0 0 0 0.23 0.17 0.16 0 0 0 0.7 0.53 0.63 0.51 0.34 0.39
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.26 0.27 0.4 0.53 0.53 0.57 0.51 0.62 0.56 0 0 0 0 0.05 0.07 0.06 0.07 0.09 0.16 0.19 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 2 3 1 1 0 1 0 0 0 0 0 1 1 3 4 3 2 14 16 6 9 9 10
Bud 2 3 8 3 8 10 14 19 13 12 32 22 2 0 1 0 3 0 3 2 2 0 3 3
Bud Neck 0 0 1 0 0 1 0 0 0 1 1 0 0 2 0 4 2 0 7 8 4 7 5 5
Bud Site 0 0 0 0 0 1 1 1 1 1 1 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 4 6 9 6 7 6 6 6 3 15 8 9 10 22 14 12 16 9 8 6 5 10 12
Cytoplasm 250 168 154 155 202 300 326 308 266 268 351 376 186 277 309 183 139 121 61 98 48 46 67 99
Endoplasmic Reticulum 130 83 42 15 4 7 5 7 6 3 3 10 61 58 59 8 6 6 391 213 182 150 101 146
Endosome 8 0 1 4 1 1 1 1 2 2 0 0 0 3 0 1 3 1 3 4 1 4 6 4
Golgi 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 4 2 0 3 0 1
Mitochondria 12 4 28 54 70 166 265 266 243 218 379 358 1 0 1 6 8 10 35 29 27 45 57 48
Nucleus 0 1 0 1 2 1 3 3 2 4 3 3 0 0 0 1 1 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 2 2 0 3 2 2 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 0 1 0 0 5 3 1 3 1 2 1 0 1 1 1 0 0 0 0 1 1 1
Vac/Vac Membrane 46 9 4 4 8 11 14 4 5 19 21 6 11 7 16 3 5 7 19 16 6 15 26 36
Unique Cell Count 424 256 223 211 257 419 504 502 424 430 614 634 260 339 375 211 162 147 556 407 289 293 297 377
Labelled Cell Count 464 276 247 248 302 508 643 618 550 536 810 795 272 358 412 227 183 166 556 407 289 293 297 377


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.2 4.2 4.4 4.3 4.0 3.6 3.7 3.5 3.4 3.3 3.4 6.3 6.0 6.0 6.0 5.8 6.2 6.8 6.5 6.8
Std Deviation (1e-4) 0.6 0.6 0.9 1.2 1.5 1.7 1.2 1.2 1.2 1.0 0.7 0.6 0.8 0.8 0.7 0.8 1.0 1.2 1.3 1.2 1.2
Intensity Change (Log2) – – – 0.07 0.01 -0.06 -0.24 -0.18 -0.25 -0.32 -0.36 -0.31 0.57 0.51 0.51 0.51 0.47 0.55 0.69 0.63 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.5 0.2 1.8 2.0 2.2 3.3
Cytoplasm 1.0 2.4 0.7 -1.1 -2.0 -1.6 -1.7 -3.1 -2.6 0.6 3.5 3.8 4.4 3.8 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.2 -0.5 -1.0 -4.9 -4.4 -4.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.6 -6.7 -6.8 -3.8 -2.5 -1.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.9 0.7 0.8 0 0 1.7 1.2 0 1.5 0 1.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5778 1.6801 1.289 1.7462 1.0176 1.4709 4.023 3.4874 3.7942 2.9408 3.658 3.5429 1.0949 1.4306 1.1889 1.0465 1.1134 1.1378
Actin 0.0251 0.0025 0.0272 0.0544 0.004 0.002 0.1372 0.0107 0.0023 0.0013 0.088 0.0032 0.003 0.0009 0.0044 0.0006 0.0011 0.0011
Bud 0.001 0.0003 0.0002 0.0006 0.0038 0 0.0006 0.0001 0.0001 0.0002 0.0005 0.0001 0 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0019 0.0007 0.001 0.0082 0.0024 0.0008 0.001 0.0012 0.0017 0.0197 0.003 0.0023 0.0002 0.0002 0.0013 0.0008 0.0007 0.0018
Bud Periphery 0.0007 0.0003 0.0006 0.001 0.0006 0.0001 0.0008 0.0003 0.0002 0.0004 0.0016 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0017 0.0231 0.0028 0.01 0.0026 0.0001 0.0032 0.0052 0.0064 0.0017 0.0072 0.0001 0.0007 0.0033 0.0017 0.0003 0.0003 0.0002
Cell Periphery 0.0012 0.0023 0.0015 0.0035 0.0007 0.0005 0.0035 0.0033 0.0042 0.0026 0.0095 0.002 0.002 0.0017 0.0019 0.0009 0.0013 0.0006
Cytoplasm 0.2989 0.4645 0.4036 0.305 0.3955 0.4655 0.2379 0.3835 0.2907 0.121 0.3838 0.4213 0.3074 0.355 0.3662 0.4366 0.38 0.4365
Cytoplasmic Foci 0.0216 0.0157 0.0076 0.0256 0.0457 0.012 0.0106 0.0164 0.0282 0.0118 0.027 0.0164 0.0066 0.0136 0.008 0.0072 0.0189 0.0086
Eisosomes 0.0008 0.0002 0.0007 0.0008 0.0001 0.0001 0.0007 0.001 0.0003 0.0003 0.0015 0.0001 0.0009 0.0002 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.2778 0.3113 0.302 0.2311 0.259 0.2621 0.4068 0.3584 0.4118 0.7447 0.2406 0.3406 0.4236 0.3642 0.3925 0.4355 0.3557 0.3605
Endosome 0.0392 0.013 0.0125 0.038 0.0131 0.0147 0.0245 0.0353 0.0105 0.0102 0.0173 0.0165 0.009 0.0295 0.0119 0.0172 0.0185 0.0147
Golgi 0.0086 0.0023 0.0048 0.0123 0.0016 0.0009 0.0127 0.0032 0.0017 0.0015 0.0065 0.0044 0.0009 0.0017 0.0014 0.0006 0.001 0.0007
Lipid Particles 0.0135 0.0068 0.0053 0.0235 0.0024 0.0045 0.0201 0.0142 0.0192 0.0044 0.0113 0.0064 0.0073 0.0079 0.0049 0.0039 0.0074 0.0033
Mitochondria 0.0033 0.0005 0.0041 0.003 0.0015 0.0006 0.0056 0.0005 0.0003 0.0013 0.0038 0.0019 0.0015 0.0011 0.0009 0.001 0.0013 0.0008
None 0.2296 0.1364 0.202 0.2291 0.2346 0.2115 0.0751 0.1455 0.2046 0.0444 0.1757 0.1727 0.2194 0.1837 0.1867 0.0534 0.1901 0.1477
Nuclear Periphery 0.0342 0.0095 0.0113 0.0188 0.0116 0.0105 0.0449 0.0078 0.0059 0.026 0.0058 0.0046 0.0088 0.018 0.0095 0.0168 0.0082 0.008
Nucleolus 0.0012 0.0003 0.0005 0.001 0.003 0.0003 0.0002 0.0002 0.0003 0.0002 0.0006 0.0001 0.0002 0.0003 0.0001 0.0006 0.0006 0.0002
Nucleus 0.0114 0.0032 0.0036 0.006 0.0082 0.0066 0.0019 0.0037 0.0014 0.0022 0.0038 0.0014 0.0024 0.0066 0.003 0.0108 0.005 0.0095
Peroxisomes 0.007 0.0003 0.001 0.0065 0.0003 0.0002 0.0039 0.0004 0.0008 0.0003 0.0035 0.0008 0.0004 0.0002 0.0002 0.0003 0.0003 0.0001
Punctate Nuclear 0.0118 0.0018 0.0029 0.0174 0.0039 0.0025 0.0026 0.0013 0.0058 0.0009 0.0021 0.001 0.0011 0.0023 0.0007 0.0068 0.0058 0.0009
Vacuole 0.0077 0.0047 0.004 0.0033 0.0048 0.0039 0.0044 0.0074 0.0032 0.004 0.0064 0.0035 0.0041 0.0083 0.0042 0.0058 0.0031 0.0039
Vacuole Periphery 0.002 0.0004 0.0009 0.001 0.0005 0.0005 0.0018 0.0005 0.0002 0.0008 0.0006 0.0003 0.0003 0.0009 0.0004 0.0008 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.6099 25.8772 28.2382 24.2474 25.3889 23.8258 25.3948 24.4112 25.2138 27.6112
Translational Efficiency 1.1768 1.2927 0.9934 1.021 1.3581 2.0632 1.58 1.2863 1.6575 1.5171

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1378 1243 1772 1141 2281 2194 1440 1490 3659 3437 3212 2631

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 574.44 784.12 884.04 912.55 768.65 783.34 877.09 915.14 695.51 783.62 880.92 914.02
Standard Deviation 70.14 110.32 108.28 113.85 84.03 106.57 121.08 117.34 122.92 107.94 114.25 115.85
Intensity Change Log 2 — 0.448918 0.621955 0.667747 — 0.027312 0.190398 0.251666 — 0.222873 0.390945 0.444465

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000447 0.000874 0.000250 0.002819 0.000261 0.000806 0.000244 0.002006 0.000331 0.000831 0.000247 0.002359
Bud Neck 0.010525 0.008719 0.005024 0.008805 0.004000 0.008221 0.004767 0.008316 0.006457 0.008401 0.004909 0.008528
Bud Site 0.005602 0.007795 0.006978 0.039011 0.002232 0.007390 0.005048 0.029486 0.003501 0.007536 0.006113 0.033617
Cell Periphery 0.002178 0.000539 0.000362 0.000730 0.000499 0.000868 0.000538 0.000655 0.001132 0.000749 0.000441 0.000688
Cytoplasm 0.301283* 0.268451* 0.431666* 0.406609* 0.330057* 0.279701* 0.448218* 0.415450* 0.319221* 0.275632* 0.439087* 0.411616*
Cytoplasmic Foci 0.129457 0.168025 0.044159 0.056632 0.123210 0.114241 0.061329 0.093635 0.125563 0.133692 0.051856 0.077588
Eisosomes 0.002430 0.000541 0.000090 0.000219 0.000830 0.001382 0.000199 0.000498 0.001432 0.001078 0.000139 0.000377
Endoplasmic Reticulum 0.198937* 0.241274* 0.074535 0.023675 0.269580* 0.321280* 0.034203 0.035104 0.242975* 0.292346* 0.056454 0.030147
Endosome 0.010043 0.024462 0.010682 0.008143 0.009636 0.017135 0.006658 0.010552 0.009789 0.019785 0.008878 0.009507
Golgi 0.029390 0.016958 0.000286 0.000303 0.022100 0.030013 0.000564 0.003037 0.024845 0.025292 0.000410 0.001852
Lipid Particles 0.160089* 0.090643 0.014500 0.009516 0.089032 0.095510 0.013390 0.016473 0.115793* 0.093750 0.014002 0.013456
Mitochondria 0.050251 0.011071 0.000606 0.000461 0.012393 0.020059 0.000998 0.002705 0.026650 0.016809 0.000782 0.001732
Mitotic Spindle 0.000104 0.000998 0.001783 0.006858 0.000294 0.001585 0.002181 0.003748 0.000222 0.001373 0.001962 0.005097
None 0.014346 0.004288 0.003122 0.002244 0.008858 0.004297 0.007599 0.001326 0.010925 0.004294 0.005129 0.001724
Nuclear Periphery 0.003726 0.007133 0.034991 0.004103 0.003874 0.002426 0.008576 0.001654 0.003818 0.004128 0.023149 0.002716
Nuclear Periphery Foci 0.002671 0.011848 0.038106 0.018734 0.003776 0.004055 0.018973 0.011972 0.003360 0.006873 0.029528 0.014904
Nucleolus 0.000671 0.000975 0.001095 0.000395 0.000681 0.000778 0.000684 0.000317 0.000677 0.000849 0.000911 0.000351
Nucleus 0.045781 0.064901 0.242555* 0.144490* 0.087346 0.049951 0.289010* 0.118211 0.071692 0.055357 0.263382* 0.129608
Peroxisomes 0.015527 0.005471 0.000577 0.001822 0.003591 0.006507 0.000752 0.001887 0.008086 0.006132 0.000655 0.001859
Vacuole 0.012717 0.061034 0.088132 0.264071* 0.026028 0.030715 0.095761 0.241861* 0.021015 0.041680 0.091552 0.251492*
Vacuole Periphery 0.003824 0.003999 0.000501 0.000359 0.001722 0.003080 0.000308 0.001107 0.002514 0.003412 0.000414 0.000783

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.45 2.41 -2.20 -1.58 -2.44 -4.30 -0.32 -5.23 -3.34 -5.18 -4.86 1.48 -4.56 -3.10 -4.72
Bud Neck 1.16 3.99 1.29 0.04 -2.94 -5.40 -1.43 -6.42 0.08 -3.91 -2.61 1.97 -2.85 0.03 -4.63
Bud Site -1.63 -1.09 -7.28 -6.50 -6.71 -5.57 -2.92 -9.03 -6.80 -7.50 -5.26 -3.33 -11.64 -9.42 -9.93
Cell Periphery 8.33 10.51 7.97 -0.33 -2.08 -6.00 -0.95 -2.35 4.16 -1.48 4.54 9.96 6.51 2.26 -2.93
Cytoplasm 3.73 -14.38 -6.05 -9.01 6.30 7.65 -12.85 -7.73 -13.73 4.22 8.27 -18.79 -9.53 -16.11 7.13
Cytoplasmic Foci -6.20 19.89 18.22 22.55 -1.97 2.10 15.85 8.49 6.35 -7.43 -2.29 26.14 17.94 19.42 -8.44
Eisosomes 5.80 7.25 6.94 6.85 -8.11 -3.40 11.34 5.90 5.96 -7.06 2.20 10.19 8.53 7.30 -9.56
Endoplasmic Reticulum -3.80 15.89 24.30 26.15 14.63 -5.46 34.85* 35.45* 38.62* 0.76 -6.76 35.72 43.10 46.76* 10.81
Endosome -10.15 -1.02 4.23 12.84 5.50 -9.05 4.93 0.18 6.97 -2.83 -13.50 1.44 2.32 13.69 1.11
Golgi 7.74 23.17 23.21 16.62 1.52 -5.44 23.43 16.62 19.78 -2.29 -0.40 33.13 27.34 25.33 -2.33
Lipid Particles 11.83 29.93 31.35 24.55 8.16 -1.74 29.26 28.13 28.33 -1.47 6.80 40.72 41.31 37.60 2.80
Mitochondria 12.78 17.24 17.29 10.04 2.10 -4.44 10.39 8.38 11.35 -1.46 6.08 19.87 18.73 14.52 -1.48
Mitotic Spindle -2.55 -2.82 -4.24 -3.58 -2.87 -2.87 -1.96 -3.55 -1.94 -1.15 -3.67 -3.26 -5.51 -3.92 -2.94
None 4.61 5.65 6.37 2.25 1.93 3.55 1.10 6.62 4.69 4.75 5.79 5.17 9.15 4.94 5.18
Nuclear Periphery -3.89 -14.09 -0.24 3.65 13.97 4.26 -5.44 6.62 2.98 8.32 -0.81 -14.85 4.15 4.09 15.85
Nuclear Periphery Foci -9.50 -18.83 -11.31 -2.91 9.83 -0.77 -10.28 -9.74 -9.03 4.66 -8.28 -21.04 -14.78 -8.51 11.41
Nucleolus -2.88 -1.51 4.11 5.92 2.48 -0.98 -0.51 6.68 4.27 5.79 -2.23 -1.58 7.76 6.43 3.58
Nucleus -5.95 -37.56 -18.49 -13.72 15.70 13.49 -31.60 -6.98 -17.42 25.28 7.63 -46.36 -16.80 -22.34 29.34
Peroxisomes 9.79 15.57 13.35 7.00 -1.66 -7.99 13.34 7.68 14.64 -6.67 4.26 18.71 14.58 14.51 -4.10
Vacuole -16.55 -25.59 -38.80 -31.70 -27.43 -2.97 -19.88 -39.21 -38.21 -21.94 -13.89 -30.95 -54.69 -49.73 -35.33
Vacuole Periphery -0.41 12.49 13.76 11.03 2.17 -6.85 8.83 1.85 4.73 -1.33 -4.52 15.12 6.87 9.50 -0.84
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
Localization
Cell Percentages ER (80%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Vph2

Vph2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vph2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available