Standard name | |
---|---|
Human Ortholog | |
Description | Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 2 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 4 | 3 | 2 | 14 | 16 | 6 | 9 | 9 | 10 |
Bud | 2 | 3 | 8 | 3 | 8 | 10 | 14 | 19 | 13 | 12 | 32 | 22 | 2 | 0 | 1 | 0 | 3 | 0 | 3 | 2 | 2 | 0 | 3 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 4 | 2 | 0 | 7 | 8 | 4 | 7 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 4 | 6 | 9 | 6 | 7 | 6 | 6 | 6 | 3 | 15 | 8 | 9 | 10 | 22 | 14 | 12 | 16 | 9 | 8 | 6 | 5 | 10 | 12 |
Cytoplasm | 250 | 168 | 154 | 155 | 202 | 300 | 326 | 308 | 266 | 268 | 351 | 376 | 186 | 277 | 309 | 183 | 139 | 121 | 61 | 98 | 48 | 46 | 67 | 99 |
Endoplasmic Reticulum | 130 | 83 | 42 | 15 | 4 | 7 | 5 | 7 | 6 | 3 | 3 | 10 | 61 | 58 | 59 | 8 | 6 | 6 | 391 | 213 | 182 | 150 | 101 | 146 |
Endosome | 8 | 0 | 1 | 4 | 1 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 3 | 0 | 1 | 3 | 1 | 3 | 4 | 1 | 4 | 6 | 4 |
Golgi | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 2 | 0 | 3 | 0 | 1 |
Mitochondria | 12 | 4 | 28 | 54 | 70 | 166 | 265 | 266 | 243 | 218 | 379 | 358 | 1 | 0 | 1 | 6 | 8 | 10 | 35 | 29 | 27 | 45 | 57 | 48 |
Nucleus | 0 | 1 | 0 | 1 | 2 | 1 | 3 | 3 | 2 | 4 | 3 | 3 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 3 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 3 | 1 | 3 | 1 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 46 | 9 | 4 | 4 | 8 | 11 | 14 | 4 | 5 | 19 | 21 | 6 | 11 | 7 | 16 | 3 | 5 | 7 | 19 | 16 | 6 | 15 | 26 | 36 |
Unique Cell Count | 424 | 256 | 223 | 211 | 257 | 419 | 504 | 502 | 424 | 430 | 614 | 634 | 260 | 339 | 375 | 211 | 162 | 147 | 556 | 407 | 289 | 293 | 297 | 377 |
Labelled Cell Count | 464 | 276 | 247 | 248 | 302 | 508 | 643 | 618 | 550 | 536 | 810 | 795 | 272 | 358 | 412 | 227 | 183 | 166 | 556 | 407 | 289 | 293 | 297 | 377 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.2 | 4.2 | 4.4 | 4.3 | 4.0 | 3.6 | 3.7 | 3.5 | 3.4 | 3.3 | 3.4 | 6.3 | 6.0 | 6.0 | 6.0 | 5.8 | 6.2 | 6.8 | 6.5 | 6.8 |
Std Deviation (1e-4) | 0.6 | 0.6 | 0.9 | 1.2 | 1.5 | 1.7 | 1.2 | 1.2 | 1.2 | 1.0 | 0.7 | 0.6 | 0.8 | 0.8 | 0.7 | 0.8 | 1.0 | 1.2 | 1.3 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.01 | -0.06 | -0.24 | -0.18 | -0.25 | -0.32 | -0.36 | -0.31 | 0.57 | 0.51 | 0.51 | 0.51 | 0.47 | 0.55 | 0.69 | 0.63 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.2 | 1.8 | 2.0 | 2.2 | 3.3 |
Cytoplasm | 1.0 | 2.4 | 0.7 | -1.1 | -2.0 | -1.6 | -1.7 | -3.1 | -2.6 | 0.6 | 3.5 | 3.8 | 4.4 | 3.8 | 2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | -0.5 | -1.0 | -4.9 | -4.4 | -4.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.6 | -6.7 | -6.8 | -3.8 | -2.5 | -1.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.9 | 0.7 | 0.8 | 0 | 0 | 1.7 | 1.2 | 0 | 1.5 | 0 | 1.6 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5778 | 1.6801 | 1.289 | 1.7462 | 1.0176 | 1.4709 | 4.023 | 3.4874 | 3.7942 | 2.9408 | 3.658 | 3.5429 | 1.0949 | 1.4306 | 1.1889 | 1.0465 | 1.1134 | 1.1378 |
Actin | 0.0251 | 0.0025 | 0.0272 | 0.0544 | 0.004 | 0.002 | 0.1372 | 0.0107 | 0.0023 | 0.0013 | 0.088 | 0.0032 | 0.003 | 0.0009 | 0.0044 | 0.0006 | 0.0011 | 0.0011 |
Bud | 0.001 | 0.0003 | 0.0002 | 0.0006 | 0.0038 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0019 | 0.0007 | 0.001 | 0.0082 | 0.0024 | 0.0008 | 0.001 | 0.0012 | 0.0017 | 0.0197 | 0.003 | 0.0023 | 0.0002 | 0.0002 | 0.0013 | 0.0008 | 0.0007 | 0.0018 |
Bud Periphery | 0.0007 | 0.0003 | 0.0006 | 0.001 | 0.0006 | 0.0001 | 0.0008 | 0.0003 | 0.0002 | 0.0004 | 0.0016 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0017 | 0.0231 | 0.0028 | 0.01 | 0.0026 | 0.0001 | 0.0032 | 0.0052 | 0.0064 | 0.0017 | 0.0072 | 0.0001 | 0.0007 | 0.0033 | 0.0017 | 0.0003 | 0.0003 | 0.0002 |
Cell Periphery | 0.0012 | 0.0023 | 0.0015 | 0.0035 | 0.0007 | 0.0005 | 0.0035 | 0.0033 | 0.0042 | 0.0026 | 0.0095 | 0.002 | 0.002 | 0.0017 | 0.0019 | 0.0009 | 0.0013 | 0.0006 |
Cytoplasm | 0.2989 | 0.4645 | 0.4036 | 0.305 | 0.3955 | 0.4655 | 0.2379 | 0.3835 | 0.2907 | 0.121 | 0.3838 | 0.4213 | 0.3074 | 0.355 | 0.3662 | 0.4366 | 0.38 | 0.4365 |
Cytoplasmic Foci | 0.0216 | 0.0157 | 0.0076 | 0.0256 | 0.0457 | 0.012 | 0.0106 | 0.0164 | 0.0282 | 0.0118 | 0.027 | 0.0164 | 0.0066 | 0.0136 | 0.008 | 0.0072 | 0.0189 | 0.0086 |
Eisosomes | 0.0008 | 0.0002 | 0.0007 | 0.0008 | 0.0001 | 0.0001 | 0.0007 | 0.001 | 0.0003 | 0.0003 | 0.0015 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.2778 | 0.3113 | 0.302 | 0.2311 | 0.259 | 0.2621 | 0.4068 | 0.3584 | 0.4118 | 0.7447 | 0.2406 | 0.3406 | 0.4236 | 0.3642 | 0.3925 | 0.4355 | 0.3557 | 0.3605 |
Endosome | 0.0392 | 0.013 | 0.0125 | 0.038 | 0.0131 | 0.0147 | 0.0245 | 0.0353 | 0.0105 | 0.0102 | 0.0173 | 0.0165 | 0.009 | 0.0295 | 0.0119 | 0.0172 | 0.0185 | 0.0147 |
Golgi | 0.0086 | 0.0023 | 0.0048 | 0.0123 | 0.0016 | 0.0009 | 0.0127 | 0.0032 | 0.0017 | 0.0015 | 0.0065 | 0.0044 | 0.0009 | 0.0017 | 0.0014 | 0.0006 | 0.001 | 0.0007 |
Lipid Particles | 0.0135 | 0.0068 | 0.0053 | 0.0235 | 0.0024 | 0.0045 | 0.0201 | 0.0142 | 0.0192 | 0.0044 | 0.0113 | 0.0064 | 0.0073 | 0.0079 | 0.0049 | 0.0039 | 0.0074 | 0.0033 |
Mitochondria | 0.0033 | 0.0005 | 0.0041 | 0.003 | 0.0015 | 0.0006 | 0.0056 | 0.0005 | 0.0003 | 0.0013 | 0.0038 | 0.0019 | 0.0015 | 0.0011 | 0.0009 | 0.001 | 0.0013 | 0.0008 |
None | 0.2296 | 0.1364 | 0.202 | 0.2291 | 0.2346 | 0.2115 | 0.0751 | 0.1455 | 0.2046 | 0.0444 | 0.1757 | 0.1727 | 0.2194 | 0.1837 | 0.1867 | 0.0534 | 0.1901 | 0.1477 |
Nuclear Periphery | 0.0342 | 0.0095 | 0.0113 | 0.0188 | 0.0116 | 0.0105 | 0.0449 | 0.0078 | 0.0059 | 0.026 | 0.0058 | 0.0046 | 0.0088 | 0.018 | 0.0095 | 0.0168 | 0.0082 | 0.008 |
Nucleolus | 0.0012 | 0.0003 | 0.0005 | 0.001 | 0.003 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0006 | 0.0002 |
Nucleus | 0.0114 | 0.0032 | 0.0036 | 0.006 | 0.0082 | 0.0066 | 0.0019 | 0.0037 | 0.0014 | 0.0022 | 0.0038 | 0.0014 | 0.0024 | 0.0066 | 0.003 | 0.0108 | 0.005 | 0.0095 |
Peroxisomes | 0.007 | 0.0003 | 0.001 | 0.0065 | 0.0003 | 0.0002 | 0.0039 | 0.0004 | 0.0008 | 0.0003 | 0.0035 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0118 | 0.0018 | 0.0029 | 0.0174 | 0.0039 | 0.0025 | 0.0026 | 0.0013 | 0.0058 | 0.0009 | 0.0021 | 0.001 | 0.0011 | 0.0023 | 0.0007 | 0.0068 | 0.0058 | 0.0009 |
Vacuole | 0.0077 | 0.0047 | 0.004 | 0.0033 | 0.0048 | 0.0039 | 0.0044 | 0.0074 | 0.0032 | 0.004 | 0.0064 | 0.0035 | 0.0041 | 0.0083 | 0.0042 | 0.0058 | 0.0031 | 0.0039 |
Vacuole Periphery | 0.002 | 0.0004 | 0.0009 | 0.001 | 0.0005 | 0.0005 | 0.0018 | 0.0005 | 0.0002 | 0.0008 | 0.0006 | 0.0003 | 0.0003 | 0.0009 | 0.0004 | 0.0008 | 0.0004 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.6099 | 25.8772 | 28.2382 | 24.2474 | 25.3889 | 23.8258 | 25.3948 | 24.4112 | 25.2138 | 27.6112 |
Translational Efficiency | 1.1768 | 1.2927 | 0.9934 | 1.021 | 1.3581 | 2.0632 | 1.58 | 1.2863 | 1.6575 | 1.5171 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1378 | 1243 | 1772 | 1141 | 2281 | 2194 | 1440 | 1490 | 3659 | 3437 | 3212 | 2631 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 574.44 | 784.12 | 884.04 | 912.55 | 768.65 | 783.34 | 877.09 | 915.14 | 695.51 | 783.62 | 880.92 | 914.02 |
Standard Deviation | 70.14 | 110.32 | 108.28 | 113.85 | 84.03 | 106.57 | 121.08 | 117.34 | 122.92 | 107.94 | 114.25 | 115.85 |
Intensity Change Log 2 | — | 0.448918 | 0.621955 | 0.667747 | — | 0.027312 | 0.190398 | 0.251666 | — | 0.222873 | 0.390945 | 0.444465 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000447 | 0.000874 | 0.000250 | 0.002819 | 0.000261 | 0.000806 | 0.000244 | 0.002006 | 0.000331 | 0.000831 | 0.000247 | 0.002359 |
Bud Neck | 0.010525 | 0.008719 | 0.005024 | 0.008805 | 0.004000 | 0.008221 | 0.004767 | 0.008316 | 0.006457 | 0.008401 | 0.004909 | 0.008528 |
Bud Site | 0.005602 | 0.007795 | 0.006978 | 0.039011 | 0.002232 | 0.007390 | 0.005048 | 0.029486 | 0.003501 | 0.007536 | 0.006113 | 0.033617 |
Cell Periphery | 0.002178 | 0.000539 | 0.000362 | 0.000730 | 0.000499 | 0.000868 | 0.000538 | 0.000655 | 0.001132 | 0.000749 | 0.000441 | 0.000688 |
Cytoplasm | 0.301283* | 0.268451* | 0.431666* | 0.406609* | 0.330057* | 0.279701* | 0.448218* | 0.415450* | 0.319221* | 0.275632* | 0.439087* | 0.411616* |
Cytoplasmic Foci | 0.129457 | 0.168025 | 0.044159 | 0.056632 | 0.123210 | 0.114241 | 0.061329 | 0.093635 | 0.125563 | 0.133692 | 0.051856 | 0.077588 |
Eisosomes | 0.002430 | 0.000541 | 0.000090 | 0.000219 | 0.000830 | 0.001382 | 0.000199 | 0.000498 | 0.001432 | 0.001078 | 0.000139 | 0.000377 |
Endoplasmic Reticulum | 0.198937* | 0.241274* | 0.074535 | 0.023675 | 0.269580* | 0.321280* | 0.034203 | 0.035104 | 0.242975* | 0.292346* | 0.056454 | 0.030147 |
Endosome | 0.010043 | 0.024462 | 0.010682 | 0.008143 | 0.009636 | 0.017135 | 0.006658 | 0.010552 | 0.009789 | 0.019785 | 0.008878 | 0.009507 |
Golgi | 0.029390 | 0.016958 | 0.000286 | 0.000303 | 0.022100 | 0.030013 | 0.000564 | 0.003037 | 0.024845 | 0.025292 | 0.000410 | 0.001852 |
Lipid Particles | 0.160089* | 0.090643 | 0.014500 | 0.009516 | 0.089032 | 0.095510 | 0.013390 | 0.016473 | 0.115793* | 0.093750 | 0.014002 | 0.013456 |
Mitochondria | 0.050251 | 0.011071 | 0.000606 | 0.000461 | 0.012393 | 0.020059 | 0.000998 | 0.002705 | 0.026650 | 0.016809 | 0.000782 | 0.001732 |
Mitotic Spindle | 0.000104 | 0.000998 | 0.001783 | 0.006858 | 0.000294 | 0.001585 | 0.002181 | 0.003748 | 0.000222 | 0.001373 | 0.001962 | 0.005097 |
None | 0.014346 | 0.004288 | 0.003122 | 0.002244 | 0.008858 | 0.004297 | 0.007599 | 0.001326 | 0.010925 | 0.004294 | 0.005129 | 0.001724 |
Nuclear Periphery | 0.003726 | 0.007133 | 0.034991 | 0.004103 | 0.003874 | 0.002426 | 0.008576 | 0.001654 | 0.003818 | 0.004128 | 0.023149 | 0.002716 |
Nuclear Periphery Foci | 0.002671 | 0.011848 | 0.038106 | 0.018734 | 0.003776 | 0.004055 | 0.018973 | 0.011972 | 0.003360 | 0.006873 | 0.029528 | 0.014904 |
Nucleolus | 0.000671 | 0.000975 | 0.001095 | 0.000395 | 0.000681 | 0.000778 | 0.000684 | 0.000317 | 0.000677 | 0.000849 | 0.000911 | 0.000351 |
Nucleus | 0.045781 | 0.064901 | 0.242555* | 0.144490* | 0.087346 | 0.049951 | 0.289010* | 0.118211 | 0.071692 | 0.055357 | 0.263382* | 0.129608 |
Peroxisomes | 0.015527 | 0.005471 | 0.000577 | 0.001822 | 0.003591 | 0.006507 | 0.000752 | 0.001887 | 0.008086 | 0.006132 | 0.000655 | 0.001859 |
Vacuole | 0.012717 | 0.061034 | 0.088132 | 0.264071* | 0.026028 | 0.030715 | 0.095761 | 0.241861* | 0.021015 | 0.041680 | 0.091552 | 0.251492* |
Vacuole Periphery | 0.003824 | 0.003999 | 0.000501 | 0.000359 | 0.001722 | 0.003080 | 0.000308 | 0.001107 | 0.002514 | 0.003412 | 0.000414 | 0.000783 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.45 | 2.41 | -2.20 | -1.58 | -2.44 | -4.30 | -0.32 | -5.23 | -3.34 | -5.18 | -4.86 | 1.48 | -4.56 | -3.10 | -4.72 |
Bud Neck | 1.16 | 3.99 | 1.29 | 0.04 | -2.94 | -5.40 | -1.43 | -6.42 | 0.08 | -3.91 | -2.61 | 1.97 | -2.85 | 0.03 | -4.63 |
Bud Site | -1.63 | -1.09 | -7.28 | -6.50 | -6.71 | -5.57 | -2.92 | -9.03 | -6.80 | -7.50 | -5.26 | -3.33 | -11.64 | -9.42 | -9.93 |
Cell Periphery | 8.33 | 10.51 | 7.97 | -0.33 | -2.08 | -6.00 | -0.95 | -2.35 | 4.16 | -1.48 | 4.54 | 9.96 | 6.51 | 2.26 | -2.93 |
Cytoplasm | 3.73 | -14.38 | -6.05 | -9.01 | 6.30 | 7.65 | -12.85 | -7.73 | -13.73 | 4.22 | 8.27 | -18.79 | -9.53 | -16.11 | 7.13 |
Cytoplasmic Foci | -6.20 | 19.89 | 18.22 | 22.55 | -1.97 | 2.10 | 15.85 | 8.49 | 6.35 | -7.43 | -2.29 | 26.14 | 17.94 | 19.42 | -8.44 |
Eisosomes | 5.80 | 7.25 | 6.94 | 6.85 | -8.11 | -3.40 | 11.34 | 5.90 | 5.96 | -7.06 | 2.20 | 10.19 | 8.53 | 7.30 | -9.56 |
Endoplasmic Reticulum | -3.80 | 15.89 | 24.30 | 26.15 | 14.63 | -5.46 | 34.85* | 35.45* | 38.62* | 0.76 | -6.76 | 35.72 | 43.10 | 46.76* | 10.81 |
Endosome | -10.15 | -1.02 | 4.23 | 12.84 | 5.50 | -9.05 | 4.93 | 0.18 | 6.97 | -2.83 | -13.50 | 1.44 | 2.32 | 13.69 | 1.11 |
Golgi | 7.74 | 23.17 | 23.21 | 16.62 | 1.52 | -5.44 | 23.43 | 16.62 | 19.78 | -2.29 | -0.40 | 33.13 | 27.34 | 25.33 | -2.33 |
Lipid Particles | 11.83 | 29.93 | 31.35 | 24.55 | 8.16 | -1.74 | 29.26 | 28.13 | 28.33 | -1.47 | 6.80 | 40.72 | 41.31 | 37.60 | 2.80 |
Mitochondria | 12.78 | 17.24 | 17.29 | 10.04 | 2.10 | -4.44 | 10.39 | 8.38 | 11.35 | -1.46 | 6.08 | 19.87 | 18.73 | 14.52 | -1.48 |
Mitotic Spindle | -2.55 | -2.82 | -4.24 | -3.58 | -2.87 | -2.87 | -1.96 | -3.55 | -1.94 | -1.15 | -3.67 | -3.26 | -5.51 | -3.92 | -2.94 |
None | 4.61 | 5.65 | 6.37 | 2.25 | 1.93 | 3.55 | 1.10 | 6.62 | 4.69 | 4.75 | 5.79 | 5.17 | 9.15 | 4.94 | 5.18 |
Nuclear Periphery | -3.89 | -14.09 | -0.24 | 3.65 | 13.97 | 4.26 | -5.44 | 6.62 | 2.98 | 8.32 | -0.81 | -14.85 | 4.15 | 4.09 | 15.85 |
Nuclear Periphery Foci | -9.50 | -18.83 | -11.31 | -2.91 | 9.83 | -0.77 | -10.28 | -9.74 | -9.03 | 4.66 | -8.28 | -21.04 | -14.78 | -8.51 | 11.41 |
Nucleolus | -2.88 | -1.51 | 4.11 | 5.92 | 2.48 | -0.98 | -0.51 | 6.68 | 4.27 | 5.79 | -2.23 | -1.58 | 7.76 | 6.43 | 3.58 |
Nucleus | -5.95 | -37.56 | -18.49 | -13.72 | 15.70 | 13.49 | -31.60 | -6.98 | -17.42 | 25.28 | 7.63 | -46.36 | -16.80 | -22.34 | 29.34 |
Peroxisomes | 9.79 | 15.57 | 13.35 | 7.00 | -1.66 | -7.99 | 13.34 | 7.68 | 14.64 | -6.67 | 4.26 | 18.71 | 14.58 | 14.51 | -4.10 |
Vacuole | -16.55 | -25.59 | -38.80 | -31.70 | -27.43 | -2.97 | -19.88 | -39.21 | -38.21 | -21.94 | -13.89 | -30.95 | -54.69 | -49.73 | -35.33 |
Vacuole Periphery | -0.41 | 12.49 | 13.76 | 11.03 | 2.17 | -6.85 | 8.83 | 1.85 | 4.73 | -1.33 | -4.52 | 15.12 | 6.87 | 9.50 | -0.84 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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