Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 13 | 6 | 11 | 5 | 7 | – | 5 | 1 | 2 | 0 | 7 | 4 | 2 | 3 | 5 | 1 | 2 | 4 | 2 | 1 | 8 |
Bud | 0 | 0 | 1 | 1 | 1 | 2 | 7 | 7 | – | 4 | 2 | 12 | 0 | 2 | 0 | 1 | 1 | 1 | 1 | 5 | 0 | 2 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | – | 6 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | – | 1 | 2 | 2 | 0 | 1 | 1 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | 0 | 9 | 6 | 9 | 17 | 21 | – | 13 | 13 | 7 | 0 | 5 | 9 | 10 | 12 | 10 | 1 | 7 | 1 | 1 | 1 | 0 |
Endoplasmic Reticulum | 0 | 1 | 1 | 5 | 2 | 3 | 4 | 6 | – | 5 | 1 | 2 | 1 | 3 | 4 | 4 | 5 | 3 | 0 | 0 | 1 | 2 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 5 | – | 8 | 2 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 1 |
Golgi | 8 | 13 | 25 | 132 | 70 | 16 | 25 | 32 | – | 25 | 5 | 5 | 16 | 93 | 144 | 69 | 42 | 64 | 16 | 24 | 29 | 20 | 15 | 29 |
Mitochondria | 2 | 24 | 33 | 113 | 339 | 405 | 508 | 599 | – | 527 | 396 | 602 | 3 | 18 | 11 | 17 | 11 | 12 | 11 | 24 | 31 | 22 | 22 | 23 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 6 | 8 | 4 | – | 0 | 4 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3 | 4 | 24 | 23 | – | 35 | 18 | 33 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 7 | 22 | 19 | 45 | 124 | 156 | 216 | 287 | – | 151 | 91 | 115 | 8 | 16 | 19 | 4 | 7 | 5 | 3 | 7 | 4 | 4 | 6 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 6 | – | 5 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | – | 12 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 6 | 15 | 6 | 38 | 73 | 57 | 47 | 61 | – | 40 | 30 | 22 | 18 | 41 | 78 | 9 | 7 | 19 | 6 | 20 | 11 | 13 | 22 | 19 |
Unique Cell Count | 17 | 60 | 60 | 242 | 465 | 518 | 663 | 844 | 643 | 462 | 657 | 34 | 143 | 213 | 94 | 69 | 99 | 51 | 102 | 96 | 77 | 90 | 101 | |
Labelled Cell Count | 24 | 77 | 85 | 358 | 626 | 672 | 873 | 1067 | 837 | 570 | 821 | 47 | 187 | 271 | 119 | 89 | 123 | 51 | 102 | 96 | 77 | 90 | 101 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 56.5 | 70.1 | 49.4 | 52.8 | 38.6 | 25.1 | 20.1 | 18.7 | – | 15.5 | 14.3 | 13.0 | 59.4 | 58.3 | 55.9 | 77.3 | 71.8 | 70.8 | 67.4 | 64.2 | 58.2 |
Std Deviation (1e-4) | 18.4 | 26.4 | 11.6 | 16.3 | 10.3 | 7.4 | 5.7 | 5.7 | – | 4.6 | 4.4 | 3.6 | 15.7 | 16.5 | 18.5 | 24.7 | 29.5 | 26.0 | 27.3 | 24.4 | 21.2 |
Intensity Change (Log2) | – | – | – | 0.1 | -0.36 | -0.97 | -1.3 | -1.4 | – | -1.67 | -1.79 | -1.92 | 0.27 | 0.24 | 0.18 | 0.65 | 0.54 | 0.52 | 0.45 | 0.38 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.8 | -5.0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.5 | 3.1 | 3.7 | 3.9 | 2.2 | 2.8 |
Mitochondria | -1.2 | 2.9 | 4.0 | 3.7 | 2.6 | – | 5.0 | 5.8 | 8.5 | -4.4 | -6.4 | -9.3 | -4.8 | -4.7 | -5.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.2 | -0.8 | -0.2 | 0.1 | 0.4 | – | -1.4 | -2.1 | -2.7 | -0.8 | -3.5 | -4.5 | -4.7 | -3.0 | -4.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.1 | 1.2 | 0.2 | 0 | 0 | – | 0 | 0 | 0 | 4.6 | 2.9 | 3.9 | -0.1 | 0 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | 0.0042 | 0.0219 | 0.0443 | 0.0631 | 0.0046 | 0.0065 | 0.0168 | 0.0222 | 0.0682 | 0.0025 | 0.0034 | 0.0167 |
Bud | – | – | – | – | – | – | 0.0114 | 0.0205 | 0.0198 | 0.0198 | 0.0002 | 0.0006 | 0.0032 | 0.0064 | 0.022 | 0.002 | 0.0001 | 0.0047 |
Bud Neck | – | – | – | – | – | – | 0.0118 | 0.0079 | 0.0015 | 0.0007 | 0.0001 | 0.0013 | 0.0005 | 0.0022 | 0.0039 | 0.0008 | 0.0003 | 0.0395 |
Bud Periphery | – | – | – | – | – | – | 0.0106 | 0.0208 | 0.0163 | 0.0089 | 0.0004 | 0.0016 | 0.0026 | 0.004 | 0.027 | 0.003 | 0.0002 | 0.0037 |
Bud Site | – | – | – | – | – | – | 0.0465 | 0.0606 | 0.0567 | 0.0106 | 0.0003 | 0.0009 | 0.0061 | 0.0383 | 0.0248 | 0.0055 | 0.0001 | 0.0012 |
Cell Periphery | – | – | – | – | – | – | 0.0182 | 0.0245 | 0.0141 | 0.0044 | 0.0002 | 0.005 | 0.0065 | 0.0163 | 0.0142 | 0.0065 | 0.0018 | 0.0083 |
Cytoplasm | – | – | – | – | – | – | 0.0045 | 0.0036 | 0.0124 | 0.0112 | 0 | 0.0001 | 0.0153 | 0.0272 | 0.0037 | 0.0002 | 0 | 0.0099 |
Cytoplasmic Foci | – | – | – | – | – | – | 0.0077 | 0.0218 | 0.0042 | 0.0366 | 0.0007 | 0.0006 | 0.1568 | 0.0089 | 0.0119 | 0.0095 | 0.0005 | 0.0062 |
Eisosomes | – | – | – | – | – | – | 0.0007 | 0.0016 | 0.0032 | 0.0035 | 0.0003 | 0.0009 | 0.0076 | 0.0029 | 0.0021 | 0.0004 | 0.0031 | 0.0015 |
Endoplasmic Reticulum | – | – | – | – | – | – | 0.0002 | 0.0002 | 0.0014 | 0.0003 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0029 |
Endosome | – | – | – | – | – | – | 0.0149 | 0.0228 | 0.011 | 0.0347 | 0.0017 | 0.0028 | 0.009 | 0.0041 | 0.0081 | 0.0028 | 0.0021 | 0.0374 |
Golgi | – | – | – | – | – | – | 0.0073 | 0.0275 | 0.0149 | 0.0214 | 0.0042 | 0.0028 | 0.006 | 0.0057 | 0.0054 | 0.0015 | 0.0023 | 0.0289 |
Lipid Particles | – | – | – | – | – | – | 0.0189 | 0.0297 | 0.0113 | 0.0066 | 0.001 | 0.0052 | 0.0567 | 0.0252 | 0.0195 | 0.0214 | 0.0202 | 0.0182 |
Mitochondria | – | – | – | – | – | – | 0.4895 | 0.3709 | 0.5855 | 0.6018 | 0.982 | 0.8479 | 0.4231 | 0.5471 | 0.5593 | 0.6687 | 0.7724 | 0.6196 |
None | – | – | – | – | – | – | 0.0005 | 0.0004 | 0.0007 | 0.0002 | 0 | 0.0001 | 0.0184 | 0.0006 | 0.0005 | 0.0002 | 0 | 0.0004 |
Nuclear Periphery | – | – | – | – | – | – | 0.0004 | 0.0004 | 0.0061 | 0.001 | 0 | 0.0001 | 0.0027 | 0.0034 | 0.0004 | 0.0002 | 0.0001 | 0.0069 |
Nucleolus | – | – | – | – | – | – | 0.1213 | 0.1148 | 0.043 | 0.0171 | 0.0001 | 0.0105 | 0.0565 | 0.1234 | 0.0255 | 0.0443 | 0.0018 | 0.0116 |
Nucleus | – | – | – | – | – | – | 0.0242 | 0.0235 | 0.0111 | 0.0042 | 0 | 0.0022 | 0.0084 | 0.0157 | 0.0066 | 0.0033 | 0.0005 | 0.0035 |
Peroxisomes | – | – | – | – | – | – | 0.0186 | 0.0105 | 0.018 | 0.0077 | 0.0017 | 0.0082 | 0.0169 | 0.0101 | 0.0081 | 0.0159 | 0.0024 | 0.0044 |
Punctate Nuclear | – | – | – | – | – | – | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0 | 0 | 0.0067 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 |
Vacuole | – | – | – | – | – | – | 0.0936 | 0.1083 | 0.0548 | 0.0388 | 0.0006 | 0.0363 | 0.0666 | 0.0574 | 0.1088 | 0.058 | 0.0276 | 0.0503 |
Vacuole Periphery | – | – | – | – | – | – | 0.0948 | 0.1076 | 0.0693 | 0.107 | 0.0017 | 0.0665 | 0.1132 | 0.0787 | 0.0793 | 0.1532 | 0.161 | 0.1241 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.8047 | 19.2217 | 18.0168 | 19.3687 | 15.4066 | 20.3593 | 20.2022 | 17.6048 | 17.4782 | 24.4277 |
Translational Efficiency | 1.4586 | 1.2851 | 1.0649 | 0.8869 | 1.2449 | 1.0119 | 1.1335 | 0.9827 | 1.1376 | 0.962 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|