Standard name | |
---|---|
Human Ortholog | |
Description | S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 2 | 4 | – | 1 | 2 | 8 | 6 | 0 | 0 | 0 | – | 0 | 0 | 1 | 5 | 2 | 0 | 0 | 1 |
Bud | 1 | 6 | 0 | 0 | 3 | 1 | 2 | – | 2 | 2 | 4 | 6 | 2 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 3 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 39 | 171 | 64 | 45 | 82 | 84 | 102 | – | 60 | 97 | 85 | 115 | 86 | 190 | 230 | – | 226 | 285 | 0 | 5 | 3 | 0 | 1 | 0 |
Cytoplasm | 160 | 477 | 229 | 139 | 237 | 256 | 292 | – | 212 | 276 | 224 | 300 | 216 | 354 | 376 | – | 408 | 411 | 150 | 461 | 214 | 133 | 318 | 324 |
Endoplasmic Reticulum | 2 | 16 | 7 | 8 | 26 | 26 | 36 | – | 20 | 19 | 11 | 22 | 4 | 6 | 9 | – | 7 | 8 | 1 | 10 | 8 | 0 | 2 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | 0 | 2 | 0 | 4 | 4 | – | 9 | 7 | 42 | 22 | 0 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 1 | 3 | 0 | 1 | 0 | 1 | 3 | – | 0 | 1 | 1 | 1 | 3 | 2 | 3 | – | 13 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 7 | 3 | 3 | – | 2 | 2 | 2 | 1 | 0 | 1 | 1 | – | 9 | 1 | 0 | 3 | 2 | 1 | 1 | 2 |
Unique Cell Count | 162 | 492 | 237 | 154 | 274 | 308 | 353 | 258 | 322 | 282 | 373 | 223 | 362 | 390 | 451 | 463 | 161 | 498 | 243 | 142 | 335 | 345 | ||
Labelled Cell Count | 204 | 675 | 301 | 197 | 355 | 377 | 447 | 306 | 407 | 377 | 474 | 311 | 553 | 621 | 667 | 728 | 161 | 498 | 243 | 142 | 335 | 345 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 16.1 | 12.3 | 11.5 | 13.3 | 12.1 | 12.3 | – | 10.6 | 10.8 | 9.3 | 10.0 | 13.2 | 14.3 | 14.5 | – | 18.2 | 17.4 | 10.6 | 12.5 | 13.5 |
Std Deviation (1e-4) | 2.3 | 3.4 | 2.0 | 2.3 | 2.0 | 2.0 | 2.2 | – | 2.0 | 1.7 | 1.5 | 1.6 | 2.2 | 2.4 | 2.4 | – | 3.2 | 3.4 | 1.8 | 2.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.11 | -0.02 | -0.0 | – | -0.22 | -0.19 | -0.41 | -0.3 | 0.1 | 0.21 | 0.24 | – | 0.57 | 0.5 | -0.22 | 0.02 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0.5 | 0.7 | 0.1 | 0.5 | – | -1.0 | 0.8 | 0.8 | 1.0 | 2.6 | 6.2 | 7.8 | – | 5.8 | 8.7 |
Cytoplasm | -2.6 | -4.0 | -5.0 | -5.2 | – | -5.2 | -4.3 | -5.9 | -5.7 | 0.1 | 0.9 | -0.1 | – | -2.9 | -3.5 |
Endoplasmic Reticulum | 1.1 | 3.0 | 2.7 | 3.3 | – | 2.3 | 1.6 | 0.6 | 1.7 | -0.8 | -1.1 | -0.5 | – | 0 | -1.1 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 6.2 | 3.8 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2072 | 9.8202 | 7.1868 | 6.9406 | 5.0523 | 7.1323 | 6.1998 | 8.4537 | 8.4395 | 7.1192 | 7.1371 | 8.4729 | 7.0638 | 10.5933 | 9.4725 | 10.5695 | 8.7684 | 9.3995 |
Actin | 0.023 | 0.0022 | 0.0325 | 0.0027 | 0.0379 | 0.0076 | 0.0193 | 0.0018 | 0.0075 | 0.0013 | 0.0113 | 0.0047 | 0.0278 | 0.0018 | 0.0115 | 0.0287 | 0.015 | 0.0046 |
Bud | 0.001 | 0.0003 | 0.0015 | 0.0003 | 0.0011 | 0.0011 | 0.0022 | 0.0008 | 0.0021 | 0.0008 | 0.0005 | 0.0019 | 0.0011 | 0.0034 | 0.0019 | 0.0028 | 0.001 | 0.004 |
Bud Neck | 0.0037 | 0.0022 | 0.0052 | 0.004 | 0.0022 | 0.008 | 0.0033 | 0.0012 | 0.0035 | 0.0052 | 0.0031 | 0.0131 | 0.006 | 0.0008 | 0.0111 | 0.0032 | 0.0208 | 0.0162 |
Bud Periphery | 0.0013 | 0.0005 | 0.0036 | 0.0004 | 0.0023 | 0.0019 | 0.0047 | 0.0009 | 0.0033 | 0.004 | 0.0015 | 0.006 | 0.0021 | 0.0024 | 0.0031 | 0.011 | 0.0025 | 0.0038 |
Bud Site | 0.0038 | 0.0114 | 0.0152 | 0.001 | 0.0071 | 0.0009 | 0.0107 | 0.0316 | 0.0295 | 0.0018 | 0.001 | 0.002 | 0.0049 | 0.0374 | 0.031 | 0.0029 | 0.0029 | 0.0005 |
Cell Periphery | 0.0092 | 0.014 | 0.0161 | 0.0037 | 0.0021 | 0.0076 | 0.0154 | 0.0106 | 0.0182 | 0.0298 | 0.0056 | 0.0095 | 0.0196 | 0.0167 | 0.0168 | 0.0139 | 0.0069 | 0.007 |
Cytoplasm | 0.8046 | 0.9594 | 0.8599 | 0.8785 | 0.7233 | 0.9281 | 0.8305 | 0.9394 | 0.893 | 0.9424 | 0.9038 | 0.9436 | 0.821 | 0.9254 | 0.8461 | 0.9107 | 0.832 | 0.9403 |
Cytoplasmic Foci | 0.0181 | 0.0011 | 0.0064 | 0.0156 | 0.0216 | 0.0047 | 0.0201 | 0.0013 | 0.0037 | 0.0005 | 0.0062 | 0.0018 | 0.0136 | 0.0012 | 0.0088 | 0.0011 | 0.0218 | 0.0023 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.027 | 0.0048 | 0.0113 | 0.0044 | 0.0072 | 0.0049 | 0.018 | 0.0054 | 0.0054 | 0.0058 | 0.0054 | 0.0077 | 0.0149 | 0.0053 | 0.0166 | 0.0106 | 0.0072 | 0.0066 |
Endosome | 0.0347 | 0.0004 | 0.0065 | 0.0444 | 0.054 | 0.0086 | 0.0164 | 0.0006 | 0.0046 | 0.0002 | 0.0212 | 0.0009 | 0.0183 | 0.0003 | 0.0118 | 0.0017 | 0.031 | 0.0018 |
Golgi | 0.0214 | 0.0003 | 0.0046 | 0.0138 | 0.0281 | 0.0048 | 0.0058 | 0.0004 | 0.0038 | 0.0002 | 0.0112 | 0.0005 | 0.0179 | 0.0004 | 0.0066 | 0.0012 | 0.0171 | 0.0034 |
Lipid Particles | 0.0179 | 0.0008 | 0.0091 | 0.007 | 0.0236 | 0.0066 | 0.0044 | 0.0004 | 0.0015 | 0.001 | 0.0094 | 0.0008 | 0.0075 | 0.0005 | 0.0048 | 0.0006 | 0.0062 | 0.0026 |
Mitochondria | 0.0085 | 0 | 0.0103 | 0.003 | 0.0335 | 0.0084 | 0.0072 | 0 | 0.0128 | 0 | 0.0082 | 0.0001 | 0.0162 | 0 | 0.0121 | 0.0005 | 0.0213 | 0.0006 |
None | 0.008 | 0.0007 | 0.0023 | 0.0011 | 0.0127 | 0.0005 | 0.0077 | 0.0019 | 0.0044 | 0.0004 | 0.0007 | 0.004 | 0.0046 | 0.0011 | 0.0025 | 0.0031 | 0.0008 | 0.0008 |
Nuclear Periphery | 0.0036 | 0.0003 | 0.0014 | 0.0005 | 0.0107 | 0.0005 | 0.009 | 0.0002 | 0.0005 | 0.0003 | 0.0011 | 0.0004 | 0.0027 | 0.0002 | 0.0031 | 0.002 | 0.0016 | 0.0003 |
Nucleolus | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0022 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0005 |
Nucleus | 0.0011 | 0.0004 | 0.0007 | 0.0005 | 0.0044 | 0.0005 | 0.0053 | 0.0005 | 0.0008 | 0.0006 | 0.0005 | 0.0007 | 0.0035 | 0.0007 | 0.003 | 0.0014 | 0.0012 | 0.0013 |
Peroxisomes | 0.0054 | 0 | 0.0058 | 0.0014 | 0.0077 | 0.0035 | 0.004 | 0 | 0.0009 | 0 | 0.0048 | 0 | 0.0055 | 0 | 0.003 | 0.0002 | 0.0053 | 0.0008 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0005 | 0.0003 | 0.0121 | 0.0003 | 0.0054 | 0.0001 | 0.0002 | 0 | 0.0015 | 0.0001 | 0.0008 | 0.0001 | 0.0012 | 0.0003 | 0.0013 | 0.0002 |
Vacuole | 0.0038 | 0.0012 | 0.0051 | 0.0155 | 0.0036 | 0.0011 | 0.0082 | 0.0027 | 0.0037 | 0.0056 | 0.002 | 0.0019 | 0.0089 | 0.002 | 0.0037 | 0.0037 | 0.0025 | 0.0021 |
Vacuole Periphery | 0.0013 | 0 | 0.0014 | 0.0017 | 0.002 | 0.0002 | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0022 | 0 | 0.0005 | 0.0002 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.0892 | 43.4527 | 33.3186 | 50.6816 | 44.1158 | 42.6435 | 57.1155 | 51.893 | 48.07 | 54.7574 |
Translational Efficiency | 1.1853 | 1.3816 | 1.3441 | 0.8181 | 0.8743 | 1.5898 | 1.0783 | 1.0217 | 1.1024 | 1.0125 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
806 | 1428 | 275 | 804 | 1719 | 1738 | 798 | 2141 | 2525 | 3166 | 1073 | 2945 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1070.26 | 1193.70 | 1735.34 | 1451.24 | 1124.65 | 1173.73 | 1597.66 | 1519.09 | 1107.29 | 1182.74 | 1632.95 | 1500.57 |
Standard Deviation | 138.19 | 207.80 | 184.61 | 346.44 | 164.64 | 201.19 | 206.46 | 233.12 | 158.72 | 204.44 | 209.88 | 270.54 |
Intensity Change Log 2 | — | 0.157479 | 0.697257 | 0.439325 | — | 0.061624 | 0.506484 | 0.433731 | — | 0.109160 | 0.602660 | 0.436461 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001415 | 0.001057 | 0.011119 | 0.021467 | 0.001528 | 0.002924 | 0.007718 | 0.002256 | 0.001492 | 0.002082 | 0.008590 | 0.007500 |
Bud Neck | 0.005552 | 0.001914 | 0.015387 | 0.005656 | 0.005022 | 0.004297 | 0.007756 | 0.005724 | 0.005191 | 0.003222 | 0.009712 | 0.005705 |
Bud Site | 0.071084 | 0.050524 | 0.094749 | 0.103701* | 0.051598 | 0.062023 | 0.065333 | 0.058546 | 0.057818 | 0.056836 | 0.072872 | 0.070874 |
Cell Periphery | 0.005167 | 0.001941 | 0.027653 | 0.016387 | 0.004312 | 0.003096 | 0.009988 | 0.001902 | 0.004585 | 0.002575 | 0.014515 | 0.005856 |
Cytoplasm | 0.821141* | 0.874055* | 0.562636* | 0.669255* | 0.866691* | 0.820274* | 0.733693* | 0.781476* | 0.852151* | 0.844531* | 0.689853* | 0.750839* |
Cytoplasmic Foci | 0.059349 | 0.036910 | 0.036951 | 0.019975 | 0.035504 | 0.049355 | 0.023222 | 0.016375 | 0.043115 | 0.043742 | 0.026741 | 0.017358 |
Eisosomes | 0.000124 | 0.000026 | 0.000142 | 0.000126 | 0.000175 | 0.000122 | 0.000132 | 0.000027 | 0.000159 | 0.000079 | 0.000135 | 0.000054 |
Endoplasmic Reticulum | 0.004968 | 0.000597 | 0.001310 | 0.000820 | 0.001000 | 0.001897 | 0.000581 | 0.000211 | 0.002267 | 0.001310 | 0.000768 | 0.000377 |
Endosome | 0.002413 | 0.001436 | 0.003643 | 0.007321 | 0.001307 | 0.004028 | 0.001227 | 0.004383 | 0.001660 | 0.002859 | 0.001846 | 0.005185 |
Golgi | 0.004230 | 0.002247 | 0.003157 | 0.017635 | 0.004177 | 0.005484 | 0.001001 | 0.002396 | 0.004194 | 0.004024 | 0.001553 | 0.006556 |
Lipid Particles | 0.001643 | 0.002164 | 0.078132 | 0.016588 | 0.002754 | 0.004032 | 0.025266 | 0.004182 | 0.002399 | 0.003190 | 0.038815 | 0.007569 |
Mitochondria | 0.001133 | 0.000107 | 0.000010 | 0.015615 | 0.000381 | 0.001107 | 0.000017 | 0.001283 | 0.000621 | 0.000656 | 0.000015 | 0.005196 |
Mitotic Spindle | 0.000336 | 0.000482 | 0.000618 | 0.007837 | 0.000812 | 0.001021 | 0.000133 | 0.002712 | 0.000660 | 0.000778 | 0.000257 | 0.004111 |
None | 0.001260 | 0.002036 | 0.000363 | 0.000301 | 0.002231 | 0.000913 | 0.000824 | 0.000176 | 0.001921 | 0.001420 | 0.000705 | 0.000210 |
Nuclear Periphery | 0.000038 | 0.000009 | 0.000108 | 0.000064 | 0.000014 | 0.000017 | 0.000045 | 0.000039 | 0.000022 | 0.000013 | 0.000061 | 0.000046 |
Nuclear Periphery Foci | 0.001652 | 0.001774 | 0.024231 | 0.004507 | 0.000885 | 0.001174 | 0.010776 | 0.000977 | 0.001129 | 0.001445 | 0.014225 | 0.001941 |
Nucleolus | 0.000049 | 0.000085 | 0.000035 | 0.000075 | 0.000119 | 0.000087 | 0.000060 | 0.000151 | 0.000097 | 0.000086 | 0.000054 | 0.000130 |
Nucleus | 0.002732 | 0.004175 | 0.007259 | 0.003678 | 0.005058 | 0.004370 | 0.006914 | 0.003755 | 0.004316 | 0.004282 | 0.007002 | 0.003734 |
Peroxisomes | 0.000744 | 0.000797 | 0.000810 | 0.026767 | 0.001003 | 0.001453 | 0.000809 | 0.001490 | 0.000920 | 0.001157 | 0.000809 | 0.008391 |
Vacuole | 0.013564 | 0.017009 | 0.131338 | 0.061102 | 0.014612 | 0.030133 | 0.104357 | 0.109544 | 0.014277 | 0.024214 | 0.111272 | 0.096319 |
Vacuole Periphery | 0.001403 | 0.000654 | 0.000346 | 0.001125 | 0.000817 | 0.002194 | 0.000150 | 0.002394 | 0.001004 | 0.001499 | 0.000200 | 0.002048 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.99 | -9.06 | -7.68 | -7.82 | -3.46 | -4.28 | -10.53 | -1.62 | 1.66 | 8.26 | -3.02 | -13.70 | -7.59 | -6.65 | 1.69 |
Bud Neck | 3.81 | -7.29 | -0.19 | -9.17 | 9.02 | 1.36 | -4.62 | -0.54 | -2.76 | 5.27 | 4.24 | -7.80 | -0.49 | -8.18 | 9.48 |
Bud Site | 4.54 | -3.25 | -4.54 | -8.09 | -0.94 | -3.64 | -4.20 | -0.86 | 2.61 | 3.38 | 0.43 | -4.85 | -3.37 | -3.84 | 1.75 |
Cell Periphery | 7.64 | -8.26 | -8.38 | -11.38 | 3.47 | 4.24 | -7.48 | 12.40 | 5.69 | 12.11 | 8.92 | -10.60 | -2.37 | -7.66 | 8.76 |
Cytoplasm | -7.53 | 16.99 | 11.84 | 16.92 | -6.37 | 8.44 | 18.60 | 17.39 | 8.05 | -6.19 | 1.90 | 23.43 | 20.16 | 18.55 | -8.19 |
Cytoplasmic Foci | 6.67 | 4.64 | 11.84 | 6.54 | 5.73 | -5.78 | 4.54 | 9.56 | 14.49 | 4.54 | -0.42 | 7.00 | 14.92 | 15.61 | 6.90 |
Eisosomes | 11.26 | -1.26 | -0.02 | -9.39 | 1.15 | 4.19 | 3.78 | 15.61 | 10.98 | 14.43 | 9.48 | 2.75 | 13.78 | 4.34 | 11.86 |
Endoplasmic Reticulum | 3.95 | 3.32 | 3.71 | -1.03 | 1.89 | -2.19 | 2.26 | 4.31 | 4.67 | 4.06 | 2.26 | 4.01 | 5.02 | 4.42 | 4.04 |
Endosome | 2.33 | -2.52 | -3.91 | -4.90 | -2.80 | -5.08 | -0.18 | -5.00 | 0.12 | -5.20 | -3.56 | -1.37 | -6.21 | -3.52 | -5.81 |
Golgi | 7.52 | 4.10 | -4.54 | -5.27 | -4.98 | -1.63 | 7.93 | 3.61 | 4.27 | -2.33 | 0.40 | 9.32 | -2.10 | -2.22 | -5.49 |
Lipid Particles | -1.86 | -14.51 | -8.85 | -8.47 | 11.01 | -2.69 | -14.91 | -3.45 | -0.78 | 13.48 | -2.55 | -19.31 | -9.12 | -7.57 | 15.76 |
Mitochondria | 1.01 | 1.10 | -4.12 | -4.65 | -4.68 | -1.84 | 3.62 | -1.30 | 0.09 | -2.05 | -0.08 | 1.83 | -4.18 | -4.29 | -5.08 |
Mitotic Spindle | -0.58 | -1.81 | -3.89 | -3.80 | -3.70 | -0.38 | 1.78 | -2.15 | -1.80 | -3.60 | -0.33 | 1.45 | -4.33 | -4.19 | -5.15 |
None | -1.00 | 1.34 | 1.38 | 4.70 | 0.24 | 3.42 | 3.77 | 6.43 | 3.24 | 2.26 | 1.38 | 3.62 | 5.50 | 5.90 | 2.25 |
Nuclear Periphery | 1.74 | -4.34 | -1.04 | -2.81 | 2.41 | -1.33 | -18.53 | -3.48 | -3.05 | 0.67 | 1.52 | -7.47 | -2.77 | -4.35 | 2.01 |
Nuclear Periphery Foci | -0.67 | -12.51 | -2.92 | -2.74 | 9.73 | -2.31 | -16.00 | -2.05 | 0.41 | 15.67 | -2.62 | -19.37 | -3.27 | -2.05 | 16.90 |
Nucleolus | -6.08 | 3.31 | -0.94 | 0.23 | -1.40 | 3.54 | 7.67 | -1.50 | -3.21 | -4.82 | 1.70 | 7.81 | -1.97 | -2.67 | -4.82 |
Nucleus | -6.86 | -11.43 | -2.70 | -0.29 | 4.85 | 2.25 | -6.35 | -0.37 | -2.49 | 5.84 | -0.05 | -10.72 | -2.26 | -2.34 | 7.50 |
Peroxisomes | -0.76 | -0.57 | -6.28 | -6.27 | -6.26 | -3.95 | 2.71 | -2.99 | 0.34 | -4.43 | -3.30 | 1.99 | -6.43 | -6.21 | -6.52 |
Vacuole | -2.83 | -17.47 | -15.38 | -14.26 | 9.82 | -11.09 | -23.56 | -33.61 | -27.18 | -1.20 | -10.54 | -29.05 | -36.18 | -31.25 | 3.54 |
Vacuole Periphery | 2.01 | 3.01 | 0.76 | -1.75 | -3.40 | -2.63 | 4.55 | -2.31 | 0.29 | -3.83 | -1.54 | 5.35 | -1.90 | -0.54 | -4.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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