Standard name | |
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Human Ortholog | |
Description | S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 2 | 4 | – | 1 | 2 | 8 | 6 | 0 | 0 | 0 | – | 0 | 0 | 1 | 5 | 2 | 0 | 0 | 1 |
Bud | 1 | 6 | 0 | 0 | 3 | 1 | 2 | – | 2 | 2 | 4 | 6 | 2 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 3 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 39 | 171 | 64 | 45 | 82 | 84 | 102 | – | 60 | 97 | 85 | 115 | 86 | 190 | 230 | – | 226 | 285 | 0 | 5 | 3 | 0 | 1 | 0 |
Cytoplasm | 160 | 477 | 229 | 139 | 237 | 256 | 292 | – | 212 | 276 | 224 | 300 | 216 | 354 | 376 | – | 408 | 411 | 150 | 461 | 214 | 133 | 318 | 324 |
Endoplasmic Reticulum | 2 | 16 | 7 | 8 | 26 | 26 | 36 | – | 20 | 19 | 11 | 22 | 4 | 6 | 9 | – | 7 | 8 | 1 | 10 | 8 | 0 | 2 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | 0 | 2 | 0 | 4 | 4 | – | 9 | 7 | 42 | 22 | 0 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 1 | 3 | 0 | 1 | 0 | 1 | 3 | – | 0 | 1 | 1 | 1 | 3 | 2 | 3 | – | 13 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 7 | 3 | 3 | – | 2 | 2 | 2 | 1 | 0 | 1 | 1 | – | 9 | 1 | 0 | 3 | 2 | 1 | 1 | 2 |
Unique Cell Count | 162 | 492 | 237 | 154 | 274 | 308 | 353 | 258 | 322 | 282 | 373 | 223 | 362 | 390 | 451 | 463 | 161 | 498 | 243 | 142 | 335 | 345 | ||
Labelled Cell Count | 204 | 675 | 301 | 197 | 355 | 377 | 447 | 306 | 407 | 377 | 474 | 311 | 553 | 621 | 667 | 728 | 161 | 498 | 243 | 142 | 335 | 345 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 16.1 | 12.3 | 11.5 | 13.3 | 12.1 | 12.3 | – | 10.6 | 10.8 | 9.3 | 10.0 | 13.2 | 14.3 | 14.5 | – | 18.2 | 17.4 | 10.6 | 12.5 | 13.5 |
Std Deviation (1e-4) | 2.3 | 3.4 | 2.0 | 2.3 | 2.0 | 2.0 | 2.2 | – | 2.0 | 1.7 | 1.5 | 1.6 | 2.2 | 2.4 | 2.4 | – | 3.2 | 3.4 | 1.8 | 2.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.11 | -0.02 | -0.0 | – | -0.22 | -0.19 | -0.41 | -0.3 | 0.1 | 0.21 | 0.24 | – | 0.57 | 0.5 | -0.22 | 0.02 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0.5 | 0.7 | 0.1 | 0.5 | – | -1.0 | 0.8 | 0.8 | 1.0 | 2.6 | 6.2 | 7.8 | – | 5.8 | 8.7 |
Cytoplasm | -2.6 | -4.0 | -5.0 | -5.2 | – | -5.2 | -4.3 | -5.9 | -5.7 | 0.1 | 0.9 | -0.1 | – | -2.9 | -3.5 |
Endoplasmic Reticulum | 1.1 | 3.0 | 2.7 | 3.3 | – | 2.3 | 1.6 | 0.6 | 1.7 | -0.8 | -1.1 | -0.5 | – | 0 | -1.1 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 6.2 | 3.8 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2072 | 9.8202 | 7.1868 | 6.9406 | 5.0523 | 7.1323 | 6.1998 | 8.4537 | 8.4395 | 7.1192 | 7.1371 | 8.4729 | 7.0638 | 10.5933 | 9.4725 | 10.5695 | 8.7684 | 9.3995 |
Actin | 0.023 | 0.0022 | 0.0325 | 0.0027 | 0.0379 | 0.0076 | 0.0193 | 0.0018 | 0.0075 | 0.0013 | 0.0113 | 0.0047 | 0.0278 | 0.0018 | 0.0115 | 0.0287 | 0.015 | 0.0046 |
Bud | 0.001 | 0.0003 | 0.0015 | 0.0003 | 0.0011 | 0.0011 | 0.0022 | 0.0008 | 0.0021 | 0.0008 | 0.0005 | 0.0019 | 0.0011 | 0.0034 | 0.0019 | 0.0028 | 0.001 | 0.004 |
Bud Neck | 0.0037 | 0.0022 | 0.0052 | 0.004 | 0.0022 | 0.008 | 0.0033 | 0.0012 | 0.0035 | 0.0052 | 0.0031 | 0.0131 | 0.006 | 0.0008 | 0.0111 | 0.0032 | 0.0208 | 0.0162 |
Bud Periphery | 0.0013 | 0.0005 | 0.0036 | 0.0004 | 0.0023 | 0.0019 | 0.0047 | 0.0009 | 0.0033 | 0.004 | 0.0015 | 0.006 | 0.0021 | 0.0024 | 0.0031 | 0.011 | 0.0025 | 0.0038 |
Bud Site | 0.0038 | 0.0114 | 0.0152 | 0.001 | 0.0071 | 0.0009 | 0.0107 | 0.0316 | 0.0295 | 0.0018 | 0.001 | 0.002 | 0.0049 | 0.0374 | 0.031 | 0.0029 | 0.0029 | 0.0005 |
Cell Periphery | 0.0092 | 0.014 | 0.0161 | 0.0037 | 0.0021 | 0.0076 | 0.0154 | 0.0106 | 0.0182 | 0.0298 | 0.0056 | 0.0095 | 0.0196 | 0.0167 | 0.0168 | 0.0139 | 0.0069 | 0.007 |
Cytoplasm | 0.8046 | 0.9594 | 0.8599 | 0.8785 | 0.7233 | 0.9281 | 0.8305 | 0.9394 | 0.893 | 0.9424 | 0.9038 | 0.9436 | 0.821 | 0.9254 | 0.8461 | 0.9107 | 0.832 | 0.9403 |
Cytoplasmic Foci | 0.0181 | 0.0011 | 0.0064 | 0.0156 | 0.0216 | 0.0047 | 0.0201 | 0.0013 | 0.0037 | 0.0005 | 0.0062 | 0.0018 | 0.0136 | 0.0012 | 0.0088 | 0.0011 | 0.0218 | 0.0023 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.027 | 0.0048 | 0.0113 | 0.0044 | 0.0072 | 0.0049 | 0.018 | 0.0054 | 0.0054 | 0.0058 | 0.0054 | 0.0077 | 0.0149 | 0.0053 | 0.0166 | 0.0106 | 0.0072 | 0.0066 |
Endosome | 0.0347 | 0.0004 | 0.0065 | 0.0444 | 0.054 | 0.0086 | 0.0164 | 0.0006 | 0.0046 | 0.0002 | 0.0212 | 0.0009 | 0.0183 | 0.0003 | 0.0118 | 0.0017 | 0.031 | 0.0018 |
Golgi | 0.0214 | 0.0003 | 0.0046 | 0.0138 | 0.0281 | 0.0048 | 0.0058 | 0.0004 | 0.0038 | 0.0002 | 0.0112 | 0.0005 | 0.0179 | 0.0004 | 0.0066 | 0.0012 | 0.0171 | 0.0034 |
Lipid Particles | 0.0179 | 0.0008 | 0.0091 | 0.007 | 0.0236 | 0.0066 | 0.0044 | 0.0004 | 0.0015 | 0.001 | 0.0094 | 0.0008 | 0.0075 | 0.0005 | 0.0048 | 0.0006 | 0.0062 | 0.0026 |
Mitochondria | 0.0085 | 0 | 0.0103 | 0.003 | 0.0335 | 0.0084 | 0.0072 | 0 | 0.0128 | 0 | 0.0082 | 0.0001 | 0.0162 | 0 | 0.0121 | 0.0005 | 0.0213 | 0.0006 |
None | 0.008 | 0.0007 | 0.0023 | 0.0011 | 0.0127 | 0.0005 | 0.0077 | 0.0019 | 0.0044 | 0.0004 | 0.0007 | 0.004 | 0.0046 | 0.0011 | 0.0025 | 0.0031 | 0.0008 | 0.0008 |
Nuclear Periphery | 0.0036 | 0.0003 | 0.0014 | 0.0005 | 0.0107 | 0.0005 | 0.009 | 0.0002 | 0.0005 | 0.0003 | 0.0011 | 0.0004 | 0.0027 | 0.0002 | 0.0031 | 0.002 | 0.0016 | 0.0003 |
Nucleolus | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0022 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0005 |
Nucleus | 0.0011 | 0.0004 | 0.0007 | 0.0005 | 0.0044 | 0.0005 | 0.0053 | 0.0005 | 0.0008 | 0.0006 | 0.0005 | 0.0007 | 0.0035 | 0.0007 | 0.003 | 0.0014 | 0.0012 | 0.0013 |
Peroxisomes | 0.0054 | 0 | 0.0058 | 0.0014 | 0.0077 | 0.0035 | 0.004 | 0 | 0.0009 | 0 | 0.0048 | 0 | 0.0055 | 0 | 0.003 | 0.0002 | 0.0053 | 0.0008 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0005 | 0.0003 | 0.0121 | 0.0003 | 0.0054 | 0.0001 | 0.0002 | 0 | 0.0015 | 0.0001 | 0.0008 | 0.0001 | 0.0012 | 0.0003 | 0.0013 | 0.0002 |
Vacuole | 0.0038 | 0.0012 | 0.0051 | 0.0155 | 0.0036 | 0.0011 | 0.0082 | 0.0027 | 0.0037 | 0.0056 | 0.002 | 0.0019 | 0.0089 | 0.002 | 0.0037 | 0.0037 | 0.0025 | 0.0021 |
Vacuole Periphery | 0.0013 | 0 | 0.0014 | 0.0017 | 0.002 | 0.0002 | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0022 | 0 | 0.0005 | 0.0002 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.0892 | 43.4527 | 33.3186 | 50.6816 | 44.1158 | 42.6435 | 57.1155 | 51.893 | 48.07 | 54.7574 |
Translational Efficiency | 1.1853 | 1.3816 | 1.3441 | 0.8181 | 0.8743 | 1.5898 | 1.0783 | 1.0217 | 1.1024 | 1.0125 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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