Standard name
Human Ortholog
Description S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.24 0.35 0.27 0.29 0.3 0.27 0.29 0.23 0.3 0.3 0.31 0.39 0.52 0.59 0.5 0.62 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.97 0.9 0.86 0.83 0.83 0.82 0.86 0.79 0.8 0.97 0.98 0.96 0.9 0.89 0.93 0.93 0.88 0.94 0.95 0.94
Endoplasmic Reticulum 0 0 0 0.05 0.09 0.08 0.1 0.08 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.15 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 2 4 1 2 8 6 0 0 0 0 0 1 5 2 0 0 1
Bud 1 6 0 0 3 1 2 2 2 4 6 2 0 1 0 0 0 2 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 1 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 39 171 64 45 82 84 102 60 97 85 115 86 190 230 226 285 0 5 3 0 1 0
Cytoplasm 160 477 229 139 237 256 292 212 276 224 300 216 354 376 408 411 150 461 214 133 318 324
Endoplasmic Reticulum 2 16 7 8 26 26 36 20 19 11 22 4 6 9 7 8 1 10 8 0 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 2 2
Golgi 0 0 0 0 0 0 1 0 1 0 0 0 0 0 2 2 0 1 0 0 0 0
Mitochondria 1 1 0 2 0 4 4 9 7 42 22 0 0 0 2 1 1 0 0 0 1 1
Nucleus 1 3 0 1 0 1 3 0 1 1 1 3 2 3 13 20 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 0 0 2 7 3 3 2 2 2 1 0 1 1 9 1 0 3 2 1 1 2
Unique Cell Count 162 492 237 154 274 308 353 258 322 282 373 223 362 390 451 463 161 498 243 142 335 345
Labelled Cell Count 204 675 301 197 355 377 447 306 407 377 474 311 553 621 667 728 161 498 243 142 335 345


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 16.1 12.3 11.5 13.3 12.1 12.3 10.6 10.8 9.3 10.0 13.2 14.3 14.5 18.2 17.4 10.6 12.5 13.5
Std Deviation (1e-4) 2.3 3.4 2.0 2.3 2.0 2.0 2.2 2.0 1.7 1.5 1.6 2.2 2.4 2.4 3.2 3.4 1.8 2.0 2.3
Intensity Change (Log2) -0.09 0.11 -0.02 -0.0 -0.22 -0.19 -0.41 -0.3 0.1 0.21 0.24 0.57 0.5 -0.22 0.02 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 0.7 0.1 0.5 -1.0 0.8 0.8 1.0 2.6 6.2 7.8 5.8 8.7
Cytoplasm -2.6 -4.0 -5.0 -5.2 -5.2 -4.3 -5.9 -5.7 0.1 0.9 -0.1 -2.9 -3.5
Endoplasmic Reticulum 1.1 3.0 2.7 3.3 2.3 1.6 0.6 1.7 -0.8 -1.1 -0.5 0 -1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 6.2 3.8 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2072 9.8202 7.1868 6.9406 5.0523 7.1323 6.1998 8.4537 8.4395 7.1192 7.1371 8.4729 7.0638 10.5933 9.4725 10.5695 8.7684 9.3995
Actin 0.023 0.0022 0.0325 0.0027 0.0379 0.0076 0.0193 0.0018 0.0075 0.0013 0.0113 0.0047 0.0278 0.0018 0.0115 0.0287 0.015 0.0046
Bud 0.001 0.0003 0.0015 0.0003 0.0011 0.0011 0.0022 0.0008 0.0021 0.0008 0.0005 0.0019 0.0011 0.0034 0.0019 0.0028 0.001 0.004
Bud Neck 0.0037 0.0022 0.0052 0.004 0.0022 0.008 0.0033 0.0012 0.0035 0.0052 0.0031 0.0131 0.006 0.0008 0.0111 0.0032 0.0208 0.0162
Bud Periphery 0.0013 0.0005 0.0036 0.0004 0.0023 0.0019 0.0047 0.0009 0.0033 0.004 0.0015 0.006 0.0021 0.0024 0.0031 0.011 0.0025 0.0038
Bud Site 0.0038 0.0114 0.0152 0.001 0.0071 0.0009 0.0107 0.0316 0.0295 0.0018 0.001 0.002 0.0049 0.0374 0.031 0.0029 0.0029 0.0005
Cell Periphery 0.0092 0.014 0.0161 0.0037 0.0021 0.0076 0.0154 0.0106 0.0182 0.0298 0.0056 0.0095 0.0196 0.0167 0.0168 0.0139 0.0069 0.007
Cytoplasm 0.8046 0.9594 0.8599 0.8785 0.7233 0.9281 0.8305 0.9394 0.893 0.9424 0.9038 0.9436 0.821 0.9254 0.8461 0.9107 0.832 0.9403
Cytoplasmic Foci 0.0181 0.0011 0.0064 0.0156 0.0216 0.0047 0.0201 0.0013 0.0037 0.0005 0.0062 0.0018 0.0136 0.0012 0.0088 0.0011 0.0218 0.0023
Eisosomes 0.0002 0 0.0006 0 0.0005 0 0.0005 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0003 0
Endoplasmic Reticulum 0.027 0.0048 0.0113 0.0044 0.0072 0.0049 0.018 0.0054 0.0054 0.0058 0.0054 0.0077 0.0149 0.0053 0.0166 0.0106 0.0072 0.0066
Endosome 0.0347 0.0004 0.0065 0.0444 0.054 0.0086 0.0164 0.0006 0.0046 0.0002 0.0212 0.0009 0.0183 0.0003 0.0118 0.0017 0.031 0.0018
Golgi 0.0214 0.0003 0.0046 0.0138 0.0281 0.0048 0.0058 0.0004 0.0038 0.0002 0.0112 0.0005 0.0179 0.0004 0.0066 0.0012 0.0171 0.0034
Lipid Particles 0.0179 0.0008 0.0091 0.007 0.0236 0.0066 0.0044 0.0004 0.0015 0.001 0.0094 0.0008 0.0075 0.0005 0.0048 0.0006 0.0062 0.0026
Mitochondria 0.0085 0 0.0103 0.003 0.0335 0.0084 0.0072 0 0.0128 0 0.0082 0.0001 0.0162 0 0.0121 0.0005 0.0213 0.0006
None 0.008 0.0007 0.0023 0.0011 0.0127 0.0005 0.0077 0.0019 0.0044 0.0004 0.0007 0.004 0.0046 0.0011 0.0025 0.0031 0.0008 0.0008
Nuclear Periphery 0.0036 0.0003 0.0014 0.0005 0.0107 0.0005 0.009 0.0002 0.0005 0.0003 0.0011 0.0004 0.0027 0.0002 0.0031 0.002 0.0016 0.0003
Nucleolus 0.0003 0 0.0001 0.0001 0.0022 0 0.0005 0 0.0001 0 0.0001 0.0001 0.0006 0.0001 0.0006 0.0001 0.0002 0.0005
Nucleus 0.0011 0.0004 0.0007 0.0005 0.0044 0.0005 0.0053 0.0005 0.0008 0.0006 0.0005 0.0007 0.0035 0.0007 0.003 0.0014 0.0012 0.0013
Peroxisomes 0.0054 0 0.0058 0.0014 0.0077 0.0035 0.004 0 0.0009 0 0.0048 0 0.0055 0 0.003 0.0002 0.0053 0.0008
Punctate Nuclear 0.0022 0.0001 0.0005 0.0003 0.0121 0.0003 0.0054 0.0001 0.0002 0 0.0015 0.0001 0.0008 0.0001 0.0012 0.0003 0.0013 0.0002
Vacuole 0.0038 0.0012 0.0051 0.0155 0.0036 0.0011 0.0082 0.0027 0.0037 0.0056 0.002 0.0019 0.0089 0.002 0.0037 0.0037 0.0025 0.0021
Vacuole Periphery 0.0013 0 0.0014 0.0017 0.002 0.0002 0.0012 0 0.0003 0.0001 0.0007 0.0001 0.0022 0 0.0005 0.0002 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.0892 43.4527 33.3186 50.6816 44.1158 42.6435 57.1155 51.893 48.07 54.7574
Translational Efficiency 1.1853 1.3816 1.3441 0.8181 0.8743 1.5898 1.0783 1.0217 1.1024 1.0125

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Ypk1

Ypk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available