Standard name
Human Ortholog
Description Putative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.96 0.95 0.93 0.89 0.73 0.69 0.72 0.72 0.67 0.67 0.98 0.96 0.98 0.97 0.97 0.98 0.98 0.93 0.91 0.9 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.13 0.13 0.19 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.08 0.24 0.29 0.13 0.17 0.15 0.2 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 1 0 0 0 2 3 2 5 4 0 0 0 0 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 0 0 5 0 7 6 6 2 4 1 1 1 0 1 2 0 0 0 0 0 0
Cytoplasm 151 318 175 116 215 190 137 298 184 233 153 145 238 293 378 132 177 159 160 302 170 119 264 248
Endoplasmic Reticulum 0 1 1 1 3 5 2 3 4 8 4 1 3 2 3 6 6 7 0 0 2 2 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0
Mitochondria 0 3 0 0 0 2 5 9 33 42 42 33 0 1 0 2 1 1 0 1 1 2 0 2
Nucleus 0 2 5 3 1 3 6 14 11 12 8 8 3 8 2 6 3 3 1 8 1 3 4 2
Nuclear Periphery 0 0 0 0 0 0 0 3 0 3 0 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 2 4 1 21 18 46 124 32 55 33 42 1 5 2 1 6 6 0 2 5 1 5 8
Unique Cell Count 153 321 183 122 231 214 188 430 255 325 227 215 242 304 384 136 183 163 163 324 187 134 285 272
Labelled Cell Count 153 326 188 123 240 223 197 460 274 361 249 241 246 310 387 147 195 178 163 324 187 134 285 272


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.3 13.8 15.7 13.9 13.9 11.0 10.0 9.5 7.7 7.8 7.1 7.1 16.3 17.2 16.2 12.1 15.1 15.8 13.6 13.6 12.5
Std Deviation (1e-4) 4.0 3.1 3.1 3.5 2.6 2.1 2.0 2.0 1.7 1.8 1.7 1.4 3.5 3.3 3.4 2.6 2.9 2.7 3.8 3.2 2.9
Intensity Change (Log2) – – – -0.17 -0.18 -0.52 -0.65 -0.72 -1.03 -1.0 -1.15 -1.14 0.06 0.14 0.05 -0.37 -0.06 0.01 -0.21 -0.21 -0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -1.1 -2.5 -6.0 -7.1 -6.3 -6.5 -7.1 -7.1 1.7 0.4 2.0 0.7 0.5 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.1 5.1 6.1 5.5 0 0 0 0 0 0
Nucleus 0 0 0 0.3 0.3 0.9 0.6 0.5 0.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.9 2.7 6.3 7.4 3.9 5.0 4.3 5.4 0 0 0 0 0.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8854 12.4278 10.9972 11.1231 10.6474 11.3243 7.9143 10.2356 9.799 8.6431 7.6186 9.4344 6.1299 9.2229 7.8899 7.3063 7.7402 8.4286
Actin 0.0202 0.0012 0.0081 0.0003 0.0024 0.0028 0.0119 0.0298 0.0119 0.0008 0.002 0.0039 0.0096 0.0007 0.006 0.0004 0.0004 0.0002
Bud 0.002 0.0001 0.0013 0.0001 0.0066 0.0003 0.0004 0.0032 0.001 0.0001 0.0004 0.0007 0.0007 0.0011 0.0005 0.0006 0.0015 0.0001
Bud Neck 0.0007 0.0002 0.0007 0.0004 0.0008 0.0015 0.0007 0.0038 0.0007 0.0004 0.0017 0.001 0.0024 0.0002 0.0004 0.0006 0.0005 0.0009
Bud Periphery 0.0009 0 0.0011 0 0.0039 0.0004 0.0004 0.0019 0.0008 0 0.0003 0.0009 0.0006 0.0004 0.0004 0.0006 0.0017 0
Bud Site 0.0021 0.0004 0.0011 0.0001 0.0001 0.0001 0.0014 0.0585 0.0087 0.0001 0.0059 0.0003 0.0007 0.0013 0.0006 0.0001 0.0001 0
Cell Periphery 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0.0007 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.8508 0.9558 0.8937 0.9776 0.8939 0.9396 0.809 0.8479 0.912 0.9833 0.8499 0.893 0.8558 0.9754 0.9432 0.9569 0.9597 0.9606
Cytoplasmic Foci 0.0116 0.0025 0.0067 0.0018 0.0265 0.0028 0.0242 0.0173 0.007 0.0028 0.0275 0.0092 0.0123 0.0011 0.0067 0.0014 0.0013 0.0026
Eisosomes 0.0002 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0117 0.0008 0.0028 0.0009 0.0016 0.0017 0.0093 0.0022 0.0015 0.0015 0.0028 0.0037 0.0048 0.0005 0.0011 0.0011 0.0004 0.0016
Endosome 0.0094 0.0004 0.0095 0.0012 0.017 0.0045 0.0296 0.0078 0.0087 0.0009 0.0238 0.0082 0.0192 0.0002 0.0021 0.0003 0.0002 0.0018
Golgi 0.0102 0.0001 0.0071 0.0001 0.011 0.0022 0.0033 0.0047 0.0047 0.0001 0.0054 0.0024 0.0029 0 0.0007 0 0 0.0001
Lipid Particles 0.0036 0 0.0079 0 0.0187 0.0005 0.001 0.0005 0.0002 0 0.0046 0.0003 0.0035 0 0.001 0 0 0.0001
Mitochondria 0.011 0.0001 0.0094 0.0001 0.0008 0.0075 0.0016 0.0006 0.0031 0.0001 0.0009 0.0029 0.0042 0.0001 0.0004 0.0002 0.0001 0.0001
None 0.0289 0.0311 0.0185 0.0091 0.0023 0.0187 0.0759 0.0085 0.0208 0.0041 0.0517 0.0479 0.0439 0.0126 0.0228 0.0257 0.024 0.016
Nuclear Periphery 0.0067 0.0007 0.0034 0.0009 0.0016 0.002 0.0077 0.0009 0.0011 0.0009 0.0012 0.003 0.0136 0.0006 0.0015 0.0013 0.0004 0.0016
Nucleolus 0.0001 0 0.0002 0 0 0 0.0002 0.0001 0 0 0.0016 0 0.0012 0 0 0 0 0
Nucleus 0.0145 0.0058 0.0234 0.0066 0.0074 0.0133 0.014 0.005 0.0051 0.0039 0.0066 0.0159 0.0135 0.0051 0.0087 0.0099 0.009 0.0126
Peroxisomes 0.0114 0.0002 0.0016 0 0.0039 0.0002 0.0016 0.0046 0.0108 0.0001 0.0027 0.0009 0.0015 0.0001 0.0018 0 0 0
Punctate Nuclear 0.001 0.0002 0.0016 0.0002 0.0009 0.0004 0.0037 0.0007 0.0005 0.0006 0.0031 0.0038 0.0064 0.0002 0.0015 0.0005 0.0001 0.0011
Vacuole 0.0019 0.0002 0.0011 0.0005 0.0005 0.0009 0.0032 0.0011 0.0009 0.0003 0.007 0.0015 0.0021 0.0003 0.0004 0.0003 0.0004 0.0004
Vacuole Periphery 0.0008 0 0.0005 0.0001 0.0002 0.0003 0.001 0.0001 0.0003 0.0001 0.0005 0.0004 0.0009 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.3242 72.201 73.3582 81.8279 93.4409 53.401 70.7561 83.0038 83.8848 96.1853
Translational Efficiency 0.4423 0.5103 0.7953 0.9061 0.7706 0.4106 0.6189 0.7316 0.7908 0.7445

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2709 521 1217 1477 1582 2176 733 1560 4291 2697 1950 3037

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1637.52 1681.28 1482.21 1340.13 1457.87 1514.20 1368.02 1395.00 1571.29 1546.48 1439.29 1368.31
Standard Deviation 258.09 343.53 300.50 311.24 284.29 253.19 318.10 337.46 281.71 280.84 312.17 326.13
Intensity Change Log 2 — 0.038047 -0.143763 -0.289140 — 0.054694 -0.091773 -0.063597 — 0.045911 -0.119043 -0.178512

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000023 0.000350 0.000235 0.001664 0.000130 0.000097 0.000438 0.003030 0.000062 0.000146 0.000311 0.002366
Bud Neck 0.000427 0.000689 0.001199 0.012025 0.000409 0.000655 0.002690 0.003040 0.000420 0.000661 0.001760 0.007410
Bud Site 0.000600 0.005354 0.001913 0.040609 0.001191 0.002281 0.005650 0.017838 0.000818 0.002875 0.003318 0.028913
Cell Periphery 0.000022 0.000122 0.000105 0.000166 0.000059 0.000035 0.000243 0.000117 0.000036 0.000051 0.000157 0.000141
Cytoplasm 0.817466* 0.774145* 0.552786* 0.595176* 0.820641* 0.822792* 0.515832* 0.703801* 0.818636* 0.813394* 0.538895* 0.650973*
Cytoplasmic Foci 0.004656 0.013944 0.002985 0.018472 0.006793 0.009531 0.012739 0.007392 0.005444 0.010383 0.006652 0.012780
Eisosomes 0.000001 0.000004 0.000003 0.000020 0.000001 0.000003 0.000036 0.000010 0.000001 0.000003 0.000015 0.000015
Endoplasmic Reticulum 0.000059 0.000608 0.000407 0.001001 0.000243 0.000265 0.002519 0.000371 0.000127 0.000331 0.001201 0.000677
Endosome 0.000056 0.001989 0.000255 0.009742 0.000196 0.000988 0.003278 0.001451 0.000107 0.001181 0.001391 0.005483
Golgi 0.000090 0.001927 0.000115 0.007471 0.000269 0.001094 0.000301 0.005080 0.000156 0.001255 0.000185 0.006243
Lipid Particles 0.000082 0.000872 0.000274 0.005027 0.000182 0.000136 0.001441 0.000568 0.000119 0.000278 0.000713 0.002737
Mitochondria 0.000106 0.000721 0.000384 0.004334 0.000627 0.000327 0.000488 0.000826 0.000298 0.000403 0.000423 0.002532
Mitotic Spindle 0.000555 0.004787 0.004239 0.086615 0.001124 0.003698 0.015824 0.034293 0.000765 0.003908 0.008593 0.059739
None 0.016963 0.015546 0.014846 0.017552 0.011135 0.011796 0.013087 0.015010 0.014815 0.012520 0.014185 0.016246
Nuclear Periphery 0.000053 0.000103 0.000369 0.000961 0.000078 0.000156 0.000973 0.000316 0.000062 0.000145 0.000596 0.000629
Nuclear Periphery Foci 0.000428 0.002525 0.000750 0.002712 0.001950 0.001381 0.004246 0.000956 0.000990 0.001602 0.002064 0.001810
Nucleolus 0.000102 0.000096 0.000163 0.000741 0.000100 0.000313 0.000538 0.000197 0.000101 0.000271 0.000304 0.000462
Nucleus 0.154245* 0.165150* 0.409732* 0.120313 0.148686* 0.127071 0.322960* 0.174229* 0.152195* 0.134427* 0.377115* 0.148008*
Peroxisomes 0.000036 0.000452 0.000030 0.001268 0.000021 0.000314 0.000412 0.000291 0.000030 0.000341 0.000173 0.000766
Vacuole 0.003976 0.009278 0.009138 0.067997 0.005824 0.016695 0.096063 0.028675 0.004658 0.015262 0.041813 0.047799
Vacuole Periphery 0.000054 0.001340 0.000072 0.006135 0.000341 0.000372 0.000242 0.002509 0.000160 0.000559 0.000136 0.004272

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.18 -5.51 -4.96 -1.87 -2.07 2.62 -10.91 -1.50 -1.62 -0.59 -3.95 -8.96 -2.78 -2.45 -1.26
Bud Neck -5.04 -10.33 -8.42 -7.21 -6.15 -3.00 -11.55 -10.21 -9.04 1.85 -4.96 -15.17 -11.95 -10.67 -5.04
Bud Site -3.83 -5.08 -9.27 -4.82 -7.51 -2.72 -8.25 -7.80 -6.96 -3.42 -5.70 -9.12 -12.22 -9.73 -8.37
Cell Periphery -9.04 -11.65 -11.08 0.26 0.50 6.17 -11.10 -7.68 -13.49 7.89 -4.22 -16.00 -14.76 -10.12 5.14
Cytoplasm 7.18 46.82 39.14 21.29 -2.86 2.46 40.94 29.55 27.72 -17.17 5.74 61.25 48.90 39.09 -13.82
Cytoplasmic Foci -7.23 0.30 -7.66 2.44 -7.67 -2.11 -1.48 -3.37 -1.40 -1.68 -7.03 -1.01 -8.33 -0.85 -7.15
Eisosomes -5.59 -10.50 -20.09 -12.29 -13.32 -5.19 -14.47 -15.82 -10.65 9.85 -7.70 -15.94 -25.61 -17.28 1.70
Endoplasmic Reticulum -2.20 -7.33 -11.11 -0.25 -1.16 -0.36 -11.57 -2.49 -2.22 11.05 -4.05 -13.25 -11.55 -2.67 8.67
Endosome -2.10 -6.80 -6.57 -0.95 -5.76 -3.05 -9.14 -4.30 0.46 5.72 -4.06 -10.42 -7.82 -1.76 -2.53
Golgi -2.29 -3.84 -3.06 0.23 -2.92 -1.86 0.22 -1.30 -0.37 -1.31 -2.92 -1.73 -2.68 -0.47 -2.59
Lipid Particles -2.34 -10.66 -6.39 -3.29 -4.98 2.60 -12.29 -10.54 -11.55 1.07 -2.29 -15.98 -9.20 -8.00 -4.89
Mitochondria -1.51 -11.33 -3.05 -1.08 -1.77 0.60 0.21 0.24 -1.37 0.21 -0.24 -1.08 -1.92 -2.12 -1.67
Mitotic Spindle -3.43 -5.22 -11.78 -10.23 -10.38 -2.89 -3.58 -10.41 -9.20 -6.04 -4.24 -5.74 -15.75 -13.80 -12.25
None 2.13 4.88 6.21 1.36 2.19 0.01 0.67 -0.75 -0.87 -1.83 3.80 4.26 4.96 0.07 0.83
Nuclear Periphery -6.57 -18.81 -19.45 -17.27 -8.34 -4.61 -24.52 -21.50 -14.91 11.21 -6.57 -29.34 -27.54 -20.88 -0.32
Nuclear Periphery Foci -6.58 -5.69 -10.72 1.55 -6.60 3.87 -5.74 0.81 -2.72 6.15 -3.85 -7.66 -7.42 -2.68 0.22
Nucleolus -0.97 -4.24 -6.77 -6.51 -5.70 -1.93 -4.88 -5.17 0.53 1.88 -1.97 -5.98 -8.61 -0.69 -3.24
Nucleus -1.74 -41.20 -2.02 0.36 35.77 3.82 -22.64 -8.57 -12.59 16.83 4.14 -45.71 -7.98 -10.62 37.52
Peroxisomes -1.45 -0.30 -5.90 0.06 -5.90 -2.75 -2.70 -4.74 0.20 -0.61 -3.05 -2.69 -7.50 -0.17 -4.75
Vacuole -6.84 -11.65 -31.86 -26.14 -25.61 -11.29 -19.66 -23.55 -17.37 5.82 -14.46 -20.75 -39.12 -30.34 -13.26
Vacuole Periphery -1.66 -1.46 -3.10 -0.11 -3.03 0.02 1.16 -0.52 -0.54 -0.84 -2.19 0.81 -2.26 -1.14 -2.42
Standard name
Human Ortholog
Description Putative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pmu1

Pmu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available