Standard name
Human Ortholog
Description Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; suggested stimulatory role in 80S formation during translation initiation; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.06 0 0 0.06 0.05 0.11 0.14 0.13 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.06 0.14 0.45 0.42 0.38 0.69 0.8 0.67 0.72 0.76 0.71 0.72 0.07 0.05 0 – – – 0 0 0 0 0 0
Nucleus 0.92 0.91 0.9 0.88 0.85 0.8 0.58 0.71 0.7 0.69 0.68 0.65 0.87 0.81 0.8 – – – 0.87 0.9 0.86 0.81 0.75 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.2 0 0 0 0.05 0 0 0 0.06 0.05 0.09 0.07 0 0 0.05 – – – 0 0 0 0.05 0.09 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.06 0 0.08 0.07 0.09 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 1 0 1
Bud 0 0 1 2 1 2 2 4 1 3 0 0 0 0 0 – – – 1 2 1 3 4 5
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 3
Bud Site 0 0 2 8 2 12 14 8 4 7 7 3 1 1 1 – – – – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 3 10 6 6 9 10 22 23 9 14 10 8 33 65 45 – – – 1 2 0 2 2 0
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 – – – 1 0 1 0 0 2
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 2 4 – – – 0 4 0 1 5 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 2 0 2
Mitochondria 11 47 109 76 98 187 349 248 208 280 120 118 22 23 9 – – – 0 1 2 4 5 6
Nucleus 178 312 216 161 219 216 255 264 203 256 116 106 262 383 271 – – – 188 315 204 241 341 264
Nuclear Periphery 3 0 1 6 1 3 5 1 2 8 3 2 3 2 0 – – – 1 0 0 0 0 5
Nucleolus 39 2 1 6 14 8 17 15 18 19 15 12 4 21 16 – – – 3 1 4 14 43 46
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 1 1 4 – – – 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 1 2 2 0 2 1 0 0 5 3 – – – 0 2 0 3 7 7
Vac/Vac Membrane 2 7 4 1 2 2 4 3 0 3 0 1 2 7 6 – – – 13 15 18 19 40 65
Unique Cell Count 193 343 240 183 257 271 438 370 288 369 170 164 301 475 340 216 352 237 297 457 428
Labelled Cell Count 238 379 342 267 348 443 670 568 445 592 273 250 328 510 359 216 352 237 297 457 428


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 5.3 4.3 4.1 4.1 3.7 2.9 3.5 3.5 3.2 3.5 3.3 6.0 5.8 5.8 – – – 5.5 5.6 6.6
Std Deviation (1e-4) 13.4 1.0 0.7 0.7 0.9 1.2 0.7 0.8 1.0 0.7 1.3 1.2 1.4 1.5 1.6 – – – 1.2 1.3 1.8
Intensity Change (Log2) – – – -0.07 -0.07 -0.22 -0.55 -0.31 -0.28 -0.43 -0.31 -0.37 0.49 0.43 0.44 – – – 0.37 0.39 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.5 0.7 0.8 1.6 2.1 0.4 0.9 1.7 1.3 3.8 4.7 4.5 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 -10.3 -13.2 -12.6 – – –
Nucleus -0.7 -1.6 -3.2 -8.6 -5.5 -5.5 -6.0 -5.5 -6.2 -1.1 -3.2 -3.3 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 2.9 3.0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0117 0 0.0085 0 0 0 0.0087 0.0008 0.0246 0 0.0444 0.0016 0.0007 0.0157 0.0045 0 0.0049 0
Bud 0.0004 0 0.0002 0 0 0 0.0011 0.0001 0.0004 0 0.0003 0.0002 0.0003 0.0019 0.0001 0 0.0004 0
Bud Neck 0.0003 0.0002 0.0009 0 0.0001 0.0004 0.0003 0.0002 0.0002 0.0001 0.0022 0.0029 0.0036 0.0019 0.0001 0 0.0003 0.0008
Bud Periphery 0.0022 0 0.0005 0 0 0.0001 0.0021 0.0001 0.001 0 0.0006 0.0006 0.0015 0.0028 0.0001 0 0.0012 0
Bud Site 0.0011 0.0001 0.001 0 0 0.0001 0.0057 0.0009 0.001 0 0.0022 0.0004 0.0003 0.0236 0.0001 0 0.0004 0
Cell Periphery 0.0015 0 0.0003 0 0 0 0.0006 0 0.0002 0 0.0003 0.0001 0.0006 0.0003 0 0 0.0004 0
Cytoplasm 0.0028 0.0001 0.0263 0 0 0 0.0411 0.074 0.0009 0.0001 0.0063 0.0071 0.0018 0.0016 0.0002 0 0.0002 0
Cytoplasmic Foci 0.0144 0 0.0037 0 0 0 0.0304 0.0347 0.0095 0 0.0436 0.0107 0 0.004 0.011 0 0.0022 0
Eisosomes 0.0046 0 0.001 0 0 0 0.0004 0 0.0002 0 0.0004 0 0 0.0001 0 0 0.0003 0
Endoplasmic Reticulum 0.0065 0 0.0013 0 0 0 0.0125 0.0041 0.0004 0 0.001 0.0006 0.0033 0.0025 0.0001 0 0.0015 0
Endosome 0.0641 0 0.0039 0 0 0 0.1129 0.0525 0.0049 0 0.0079 0.0061 0.0012 0.0083 0.0127 0 0.079 0
Golgi 0.0215 0 0.0012 0 0 0 0.0129 0.0236 0.0025 0 0.0075 0.0008 0 0.005 0.0489 0 0.0813 0
Lipid Particles 0.4748 0 0.0042 0 0 0 0.0779 0.0328 0.0042 0 0.009 0.0012 0 0.0009 0.0053 0 0.0113 0
Mitochondria 0.1985 0.0001 0.01 0.0001 0.0002 0.0001 0.0099 0.0025 0.0173 0.0001 0.0017 0.0034 0.0021 0.0207 0.0042 0.0001 0.7758 0.0001
None 0.0499 0 0.0705 0 0 0 0.0987 0.0045 0.0047 0 0.0062 0.0079 0.0002 0.0005 0 0 0.0002 0
Nuclear Periphery 0.0606 0.0002 0.0018 0.0004 0.0004 0.0002 0.0415 0.0043 0.0028 0.0005 0.0016 0.0189 0.054 0.0044 0.001 0.0004 0.0017 0.0001
Nucleolus 0.0048 0.0122 0.0092 0.0032 0.0185 0.0352 0.005 0.0055 0.0177 0.0089 0.1991 0.0294 0.0133 0.0136 0.0138 0.0118 0.0002 0.0338
Nucleus 0.0032 0.9868 0.849 0.9961 0.9804 0.9632 0.5087 0.7451 0.8658 0.99 0.6457 0.8669 0.901 0.8793 0.8937 0.9871 0.0005 0.9645
Peroxisomes 0.0294 0 0.0033 0 0 0 0.0054 0.0082 0.0146 0 0.0123 0.0045 0 0.0033 0.0036 0 0.0008 0
Punctate Nuclear 0.0059 0.0001 0.001 0 0.0001 0.0004 0.0064 0.0034 0.0226 0.0003 0.0069 0.033 0.0006 0.0054 0.0003 0.0005 0.0001 0.0005
Vacuole 0.0174 0.0001 0.0015 0 0.0001 0.0001 0.0112 0.0021 0.0024 0 0.0006 0.0016 0.0109 0.0021 0.0001 0 0.0103 0
Vacuole Periphery 0.0243 0 0.0005 0 0 0.0001 0.0066 0.0006 0.0023 0 0.0003 0.002 0.0045 0.0021 0.0002 0 0.0269 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0815 13.0522 8.3017 14.8665 17.1277 6.9489 11.626 12.5124 11.8451 15.0445
Translational Efficiency 1.3455 0.9876 1.4921 0.8023 0.8427 2.0429 1.2287 0.9756 1.125 0.9971

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1970 1345 719 1775 1541 2005 194 889 3511 3350 913 2664

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 703.93 843.73 969.13 999.10 776.71 831.54 1051.48 1044.25 735.87 836.43 986.63 1014.17
Standard Deviation 93.85 138.87 127.13 141.45 91.32 120.24 110.49 148.65 99.53 128.18 128.28 145.46
Intensity Change Log 2 — 0.261349 0.461258 0.505197 — 0.098410 0.436973 0.427019 — 0.178173 0.448570 0.464715

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000123 0.000494 0.000614 0.000824 0.000172 0.000365 0.001443 0.001390 0.000144 0.000417 0.000790 0.001013
Bud Neck 0.003561 0.006801 0.010253 0.030933 0.003083 0.011451 0.004906 0.030331 0.003351 0.009584 0.009117 0.030732
Bud Site 0.001472 0.001233 0.000840 0.004967 0.000289 0.001989 0.001172 0.007759 0.000952 0.001685 0.000911 0.005899
Cell Periphery 0.000224 0.000132 0.000060 0.000165 0.000046 0.000139 0.000084 0.000143 0.000146 0.000136 0.000065 0.000158
Cytoplasm 0.015187 0.005668 0.003473 0.010847 0.001999 0.004560 0.000776 0.008731 0.009398 0.005005 0.002900 0.010141
Cytoplasmic Foci 0.002611 0.001236 0.000208 0.000952 0.000590 0.000905 0.000047 0.003141 0.001724 0.001038 0.000174 0.001682
Eisosomes 0.000015 0.000020 0.000030 0.000028 0.000013 0.000024 0.000052 0.000036 0.000014 0.000022 0.000034 0.000031
Endoplasmic Reticulum 0.001719 0.005399 0.004311 0.005383 0.002274 0.004817 0.010958 0.005610 0.001963 0.005050 0.005723 0.005459
Endosome 0.000297 0.001347 0.000193 0.001954 0.000209 0.001080 0.000213 0.006200 0.000258 0.001187 0.000197 0.003371
Golgi 0.000110 0.001204 0.000056 0.000978 0.000230 0.000476 0.000072 0.008993 0.000162 0.000768 0.000059 0.003652
Lipid Particles 0.000418 0.000617 0.000204 0.000354 0.000194 0.000745 0.000250 0.000280 0.000320 0.000694 0.000214 0.000330
Mitochondria 0.001311 0.003107 0.000844 0.004756 0.001017 0.004143 0.001026 0.011711 0.001182 0.003727 0.000883 0.007077
Mitotic Spindle 0.001017 0.003915 0.000278 0.017203 0.001193 0.006991 0.000161 0.027911 0.001094 0.005756 0.000253 0.020776
None 0.018244 0.008585 0.002860 0.005680 0.002668 0.005590 0.002736 0.002915 0.011408 0.006792 0.002833 0.004757
Nuclear Periphery 0.000485 0.000481 0.000374 0.002591 0.000307 0.001561 0.000295 0.003450 0.000407 0.001128 0.000357 0.002878
Nuclear Periphery Foci 0.000296 0.000796 0.000085 0.000837 0.000165 0.000589 0.000107 0.001843 0.000239 0.000672 0.000090 0.001173
Nucleolus 0.014686 0.011442 0.006114 0.003589 0.011030 0.014271 0.005764 0.004110 0.013081 0.013135 0.006039 0.003763
Nucleus 0.937184* 0.945035* 0.966909* 0.898930* 0.973732* 0.936141* 0.965913* 0.858183* 0.953225* 0.939712* 0.966697* 0.885332*
Peroxisomes 0.000234 0.000782 0.000278 0.000173 0.000520 0.000666 0.000436 0.000570 0.000360 0.000713 0.000311 0.000305
Vacuole 0.000659 0.001114 0.001926 0.006869 0.000181 0.002630 0.003477 0.011542 0.000450 0.002021 0.002256 0.008429
Vacuole Periphery 0.000148 0.000592 0.000089 0.001987 0.000089 0.000867 0.000115 0.005150 0.000122 0.000756 0.000094 0.003043

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.51 -10.93 -16.45 -7.73 -3.30 -6.69 -7.38 -9.87 -8.51 -0.57 -11.20 -12.69 -17.25 -12.30 -3.61
Bud Neck -5.57 -10.40 -16.24 -14.05 -10.38 -7.95 -3.99 -11.11 -7.60 -9.78 -9.83 -10.83 -19.63 -14.72 -13.26
Bud Site 0.31 0.55 -3.63 -4.81 -5.14 -4.27 -4.69 -7.16 -5.38 -6.05 -1.89 -0.72 -7.40 -6.62 -7.87
Cell Periphery 2.35 4.41 2.41 0.02 -5.28 -4.58 -1.73 -7.48 0.41 -2.31 0.41 3.76 0.68 0.33 -6.23
Cytoplasm 4.70 7.76 3.26 -1.83 -4.71 -3.15 1.83 -3.93 -2.10 -4.76 3.94 6.84 0.44 -3.25 -5.93
Cytoplasmic Foci 1.90 3.40 2.93 1.77 -1.01 -0.44 1.98 -1.28 -1.06 -3.36 1.73 3.75 2.21 0.86 -2.72
Eisosomes -3.93 -9.14 -15.89 -8.08 0.16 -7.70 -11.01 -15.24 -8.73 2.47 -8.40 -13.36 -22.19 -10.90 0.53
Endoplasmic Reticulum -12.14 -9.70 -11.40 -1.18 -2.05 -9.36 -4.06 -11.43 -4.66 1.85 -15.26 -7.94 -15.39 -3.73 -0.05
Endosome -2.37 1.85 -3.51 0.33 -3.83 -3.21 -0.30 -4.11 -2.74 -4.08 -3.92 0.82 -5.37 -1.69 -5.59
Golgi -2.00 1.35 -1.88 1.12 -2.12 -0.96 0.99 -2.62 -2.47 -2.75 -2.37 1.42 -3.02 -1.69 -3.26
Lipid Particles -0.54 1.84 0.94 1.02 -0.76 -2.35 -1.13 -2.17 1.82 -0.50 -1.91 1.26 0.23 2.01 -0.96
Mitochondria -1.50 0.31 -3.77 -0.85 -5.36 -4.27 -0.28 -3.96 -1.92 -3.98 -3.86 0.13 -5.63 -1.72 -6.29
Mitotic Spindle -1.98 1.38 -6.53 -4.42 -7.01 -3.63 1.60 -5.56 -3.77 -5.94 -4.25 2.09 -8.55 -5.41 -9.18
None 4.38 8.86 7.64 2.45 -2.88 -2.94 0.37 -1.10 2.39 -0.93 3.77 8.31 6.92 2.88 -2.92
Nuclear Periphery 0.07 0.35 -6.00 -5.94 -6.12 -4.09 -2.27 -3.12 -1.38 -2.92 -3.82 -1.01 -6.30 -3.61 -6.14
Nuclear Periphery Foci -1.69 1.93 -2.00 0.53 -3.83 -2.33 0.14 -2.47 -1.52 -2.49 -2.64 2.01 -3.28 -0.84 -4.12
Nucleolus 2.45 6.81 9.40 7.31 2.64 -2.29 4.36 4.65 7.58 -0.13 0.11 7.67 10.52 11.27 2.16
Nucleus -1.54 -6.47 5.96 6.93 12.06 8.85 2.43 12.10 7.12 9.88 3.83 -3.49 13.07 9.57 15.68
Peroxisomes -1.65 -0.24 1.43 1.98 1.53 -0.36 0.88 0.71 1.44 -0.23 -1.77 0.81 1.49 2.99 0.82
Vacuole -1.10 -2.16 -6.10 -5.79 -3.53 -5.57 -5.34 -6.52 -5.31 -3.99 -4.95 -3.74 -8.99 -7.34 -5.38
Vacuole Periphery -2.29 2.20 -3.42 -1.48 -3.62 -5.32 -1.49 -3.35 -2.19 -3.29 -5.42 1.17 -4.76 -2.44 -4.83
Standard name
Human Ortholog
Description Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; suggested stimulatory role in 80S formation during translation initiation; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ctk1

Ctk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available