Standard name
Human Ortholog
Description Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0.08 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0.06 0 0.07 – 0.05 0.1 0 0.1 0 0.05 0.05 0 0.1 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.96 0.88 0.84 0.78 – 0.77 0.66 0.73 0.65 0.97 0.98 0.98 0.94 0.92 0.95 0.96 0.93 0.95 0.92 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.16 – 0.13 0.15 0.08 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0 – 0.06 0.06 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0 – 0 0.06 0.09 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 1 1 3 5 – 8 20 16 20 0 0 1 0 0 0 1 1 0 0 1 1
Bud 0 1 0 0 0 1 0 – 1 1 1 1 0 0 0 0 0 0 1 1 1 2 4 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 7 6 6 10 1 16 – 12 24 9 23 11 22 20 7 14 6 0 0 0 0 0 0
Cytoplasm 364 304 182 99 152 67 170 – 174 156 146 151 288 423 433 149 134 115 351 286 171 199 387 395
Endoplasmic Reticulum 6 4 2 2 8 2 35 – 30 35 16 34 8 10 12 6 5 2 1 2 3 6 12 11
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0
Golgi 1 0 0 0 3 0 1 – 2 13 1 7 0 1 0 0 0 1 1 0 0 0 0 1
Mitochondria 2 1 0 0 1 9 1 – 14 15 19 29 0 0 0 4 2 4 2 1 1 1 0 1
Nucleus 2 0 2 0 0 2 0 – 2 1 0 1 0 0 0 1 0 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 3 12 0 6 – 9 14 18 8 0 0 3 8 8 1 0 5 0 1 3 1
Unique Cell Count 375 309 184 103 173 80 217 227 237 201 232 296 431 444 159 145 121 365 308 181 218 417 421
Labelled Cell Count 387 320 193 111 188 86 235 253 279 227 274 307 456 469 175 163 132 365 308 181 218 417 421


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 15.3 14.9 13.8 17.1 11.8 17.7 – 16.5 17.6 16.5 17.6 20.7 24.0 26.4 20.4 21.9 20.8 13.6 15.5 17.2
Std Deviation (1e-4) 2.6 3.3 2.3 2.6 2.7 3.6 3.8 – 4.4 4.6 4.8 5.0 4.5 5.9 5.7 6.9 7.7 7.2 3.9 4.0 3.7
Intensity Change (Log2) – – – -0.11 0.2 -0.34 0.25 – 0.15 0.25 0.15 0.25 0.48 0.69 0.83 0.46 0.55 0.48 -0.13 0.06 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 4.0 3.9 4.1 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.1 0 1.8 – 1.0 2.7 0.6 2.6 0.3 1.0 0.7 0.6 2.4 0
Cytoplasm -1.6 -4.3 -4.9 -6.3 – -6.6 -8.5 -7.3 -8.6 -1.2 -0.7 -1.1 -2.6 -3.0 -2.1
Endoplasmic Reticulum 0 0 0 5.2 – 4.6 4.9 3.2 4.9 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 3.2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.4 3.5 4.3 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.2 0 0 – 0 2.9 3.8 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.9927 17.0823 15.0946 13.6309 13.9601 15.8587 14.3326 17.7574 16.1533 16.0164 16.492 15.9635 21.3565 24.6676 23.0513 20.3686 22.1744 22.8382
Actin 0.0082 0.0028 0.0007 0.0005 0.0005 0.001 0.0558 0.0017 0.0338 0.0023 0.0002 0.0144 0.0005 0.0023 0.0011 0.006 0.0001 0.001
Bud 0.0003 0.0006 0.0006 0.0008 0.0006 0.0006 0.0028 0.0002 0.0025 0.0003 0.0002 0.0009 0.0002 0.0004 0.0003 0.0036 0.0001 0.0004
Bud Neck 0.0004 0.0002 0.0004 0.0005 0.0005 0.0008 0.0125 0.0002 0.0006 0.0005 0.0006 0.0011 0.0004 0.0002 0.0003 0.0004 0.0004 0.0013
Bud Periphery 0.0002 0.0002 0.0002 0.0002 0.0004 0.0002 0.0028 0.0001 0.0017 0 0 0.0013 0.0001 0.0001 0.0001 0.0046 0 0.0005
Bud Site 0.0005 0.0025 0.0004 0.0004 0.0002 0.0001 0.0316 0.0006 0.0078 0.0003 0.0001 0.0006 0.0002 0.0005 0.0003 0.0002 0 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0017 0.0001 0.0002 0 0 0.0001 0.0003 0.0002 0.0001 0.0002 0 0.0001
Cytoplasm 0.9072 0.9793 0.9485 0.9008 0.9221 0.9491 0.7569 0.9807 0.8968 0.9716 0.9174 0.8949 0.9169 0.9763 0.9612 0.885 0.9835 0.948
Cytoplasmic Foci 0.0083 0.0036 0.0071 0.0108 0.012 0.0071 0.0126 0.0025 0.0106 0.0111 0.0087 0.0132 0.004 0.0025 0.0053 0.0057 0.0025 0.0074
Eisosomes 0.0001 0 0 0 0 0 0.0006 0 0.0001 0 0 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0268 0.0026 0.0087 0.0028 0.0093 0.0154 0.0264 0.0042 0.0097 0.0012 0.0024 0.0103 0.0472 0.0087 0.0091 0.0092 0.0044 0.0134
Endosome 0.0181 0.0029 0.0154 0.0384 0.0306 0.017 0.0194 0.0028 0.0141 0.0055 0.0546 0.0257 0.0132 0.0034 0.0126 0.0209 0.0039 0.0147
Golgi 0.0027 0.0013 0.0015 0.0025 0.0015 0.0017 0.0095 0.0037 0.0118 0.0008 0.0016 0.0094 0.0008 0.0008 0.0018 0.0029 0.0001 0.0021
Lipid Particles 0.0023 0.0001 0.0001 0.0001 0 0 0.0044 0.0001 0.0006 0.0001 0 0.0044 0.0003 0 0.0001 0 0 0
Mitochondria 0.0011 0.0001 0.0062 0.0006 0.0109 0.0016 0.0139 0.0004 0.0028 0.0001 0.0003 0.0145 0.0004 0.0006 0.0005 0.04 0.0001 0.0064
None 0.0015 0.0004 0.0004 0.0004 0.0008 0.0004 0.0027 0.0002 0.0005 0.0004 0.0004 0.0005 0.0019 0.0002 0.0004 0.0007 0.0003 0.0004
Nuclear Periphery 0.0051 0.0005 0.0017 0.0019 0.0016 0.0007 0.0085 0.0005 0.0017 0.0005 0.0007 0.0017 0.004 0.0006 0.0011 0.0019 0.0012 0.0009
Nucleolus 0.0001 0 0 0 0 0 0.0009 0 0 0 0 0.0001 0 0 0 0 0 0
Nucleus 0.0016 0.0007 0.0011 0.0019 0.0014 0.0009 0.0065 0.0006 0.001 0.001 0.0008 0.0013 0.0015 0.0006 0.0008 0.0014 0.0009 0.0007
Peroxisomes 0.0012 0.0001 0.0001 0.0001 0.0001 0.0001 0.002 0.0001 0.0008 0.0002 0 0.0009 0 0 0 0.0001 0 0.0001
Punctate Nuclear 0.0012 0.0001 0.0001 0.0001 0.0001 0.0001 0.0077 0 0.0001 0.0002 0.0001 0.0004 0.0003 0 0.0001 0.0001 0 0
Vacuole 0.0109 0.0016 0.0055 0.0316 0.0048 0.0023 0.0183 0.001 0.0021 0.0032 0.0072 0.0025 0.0067 0.002 0.0041 0.0125 0.0017 0.0017
Vacuole Periphery 0.002 0.0002 0.0012 0.0053 0.0022 0.0009 0.0026 0.0002 0.0007 0.0007 0.0046 0.0016 0.0011 0.0004 0.0008 0.0047 0.0007 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 89.4426 59.7804 78.7383 93.4987 58.525 150.3809 105.0212 120.3468 155.9854 109.8588
Translational Efficiency 2.1791 2.1087 2.043 2.629 2.7452 2.2387 2.0669 2.2054 2.0671 2.1827

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
149 1696 165 2448 2163 1981 1350 128 2312 3677 1515 2576

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2925.08 1372.92 2576.67 1790.00 1312.72 1328.99 1755.51 2426.46 1416.63 1349.25 1844.94 1821.63
Standard Deviation 821.41 253.90 466.11 489.88 244.26 222.17 357.41 392.36 506.01 238.34 450.48 504.81
Intensity Change Log 2 — -1.091229 -0.182968 -0.708516 — 0.017771 0.419331 0.886294 — -0.649336 0.031778 -0.007283

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001031 0.000201 0.000502 0.000150 0.000117 0.000287 0.000082 0.000948 0.000176 0.000247 0.000128 0.000190
Bud Neck 0.001248 0.000272 0.005733 0.001590 0.000286 0.000491 0.000381 0.009619 0.000348 0.000390 0.000964 0.001989
Bud Site 0.025739 0.009037 0.019247 0.008892 0.008670 0.014997 0.005940 0.027267 0.009770 0.012248 0.007389 0.009805
Cell Periphery 0.060289 0.000186 0.021830 0.000481 0.000143 0.000186 0.000661 0.005976 0.004020 0.000186 0.002966 0.000754
Cytoplasm 0.353143* 0.927676* 0.708612* 0.806438* 0.906386* 0.889449* 0.954438* 0.479793* 0.870732* 0.907081* 0.927665* 0.790207*
Cytoplasmic Foci 0.038784 0.032358 0.030474 0.011706 0.053902 0.059599 0.003914 0.048598 0.052928 0.047035 0.006806 0.013539
Eisosomes 0.000013 0.000002 0.000007 0.000040 0.000002 0.000004 0.000001 0.000010 0.000003 0.000003 0.000001 0.000039
Endoplasmic Reticulum 0.006711 0.000956 0.000556 0.000654 0.001601 0.001210 0.000091 0.002462 0.001930 0.001093 0.000142 0.000744
Endosome 0.006192 0.001445 0.003243 0.001939 0.002140 0.002508 0.000398 0.016173 0.002401 0.002018 0.000708 0.002646
Golgi 0.224185* 0.006857 0.033880 0.002915 0.006426 0.007486 0.001837 0.053463 0.020460 0.007196 0.005326 0.005427
Lipid Particles 0.060470 0.001172 0.017621 0.001554 0.000617 0.000808 0.001559 0.023842 0.004474 0.000976 0.003309 0.002661
Mitochondria 0.000255 0.000655 0.000082 0.000144 0.000408 0.000467 0.000061 0.000023 0.000398 0.000553 0.000063 0.000138
Mitotic Spindle 0.004469 0.000731 0.006483 0.000909 0.001499 0.002381 0.000208 0.002694 0.001691 0.001620 0.000891 0.000998
None 0.000845 0.001445 0.000853 0.006399 0.001083 0.001182 0.001066 0.000244 0.001067 0.001303 0.001043 0.006093
Nuclear Periphery 0.000502 0.000021 0.000531 0.000101 0.000020 0.000015 0.000019 0.000304 0.000051 0.000018 0.000075 0.000111
Nuclear Periphery Foci 0.013103 0.001173 0.012673 0.002062 0.000646 0.000717 0.001329 0.017987 0.001449 0.000927 0.002564 0.002853
Nucleolus 0.000196 0.000181 0.000088 0.000346 0.000046 0.000139 0.000062 0.000114 0.000056 0.000158 0.000065 0.000334
Nucleus 0.003158 0.004133 0.023505 0.057000 0.003906 0.004433 0.006823 0.010269 0.003858 0.004295 0.008640 0.054678
Peroxisomes 0.003233 0.000253 0.000055 0.000207 0.000168 0.000341 0.000118 0.000446 0.000366 0.000301 0.000111 0.000219
Vacuole 0.075815 0.008711 0.099927 0.092862 0.008527 0.010658 0.019295 0.284922* 0.012864 0.009760 0.028076 0.102405
Vacuole Periphery 0.120618* 0.002536 0.014098 0.003612 0.003404 0.002640 0.001719 0.014847 0.010958 0.002592 0.003067 0.004171

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.37 0.85 1.43 0.75 5.38 -1.86 0.80 -6.61 -4.41 -6.63 -0.84 0.77 -0.39 0.74 -1.83
Bud Neck 5.31 -5.15 -1.54 -8.41 4.86 -4.65 -1.35 -8.32 -8.16 -8.23 -1.14 -5.17 -10.16 -9.95 -3.88
Bud Site 3.69 0.67 3.70 0.13 3.83 -5.88 3.07 -5.18 -3.45 -5.83 -3.01 2.15 -0.09 2.55 -2.01
Cell Periphery 8.28 4.81 8.24 -5.42 4.98 -0.90 -3.93 -4.36 -4.32 -3.92 6.86 1.64 5.87 -6.28 4.35
Cytoplasm -20.15 -9.27 -15.42 22.25 -5.02 3.37 -11.64 15.56 14.91 17.36 -6.89 -8.99 13.27 22.43 22.81
Cytoplasmic Foci 1.42 0.64 5.97 10.38 6.02 -1.82 22.47 0.89 1.92 -8.92 2.30 21.15 17.87 20.03 -7.22
Eisosomes 2.44 1.33 -3.51 -7.58 -6.29 -4.72 8.27 -7.06 -4.88 -8.36 -0.92 3.77 -7.51 -7.43 -7.85
Endoplasmic Reticulum 2.04 2.19 2.16 1.34 -0.59 0.80 4.89 -0.80 -1.23 -2.87 2.04 5.24 3.41 1.51 -4.89
Endosome 6.55 3.01 5.84 -2.02 2.81 -1.17 8.17 -7.71 -7.45 -8.77 1.62 7.80 -0.72 -2.25 -7.92
Golgi 13.91 11.95 14.19 5.34 8.79 -1.26 9.65 -4.61 -4.48 -5.13 8.11 9.38 9.40 2.95 0.37
Lipid Particles 7.77 5.25 7.70 -1.85 7.26 -1.51 -3.37 -7.25 -7.18 -6.92 5.87 1.50 2.66 -6.77 1.40
Mitochondria -0.90 1.38 1.01 1.28 -2.66 -0.18 1.26 1.38 2.86 5.11 -0.46 1.30 1.02 2.06 -5.54
Mitotic Spindle 3.98 -1.36 3.54 -1.42 3.25 -1.11 3.64 -1.27 -0.42 -2.75 0.28 1.76 1.34 1.01 -0.57
None -2.68 -0.01 -5.08 -4.45 -5.11 -0.42 0.14 6.32 4.43 6.47 -1.38 0.21 -4.83 -4.56 -4.87
Nuclear Periphery 4.86 -0.85 3.89 -4.42 6.38 1.04 0.14 -6.54 -6.68 -6.55 3.93 -2.42 -3.43 -5.48 -1.71
Nuclear Periphery Foci 8.68 0.00 7.86 -3.09 5.60 -0.47 -2.10 -5.13 -5.10 -4.89 2.79 -3.00 -4.65 -6.19 -0.75
Nucleolus 0.08 0.81 -0.80 -0.91 -3.77 -1.38 -1.00 -3.32 0.21 -3.45 -1.44 -0.52 -4.35 -1.69 -4.37
Nucleus -1.19 -6.02 -20.50 -21.00 -6.20 -0.84 -5.69 -3.38 -3.20 -2.40 -1.27 -7.98 -21.36 -21.07 -18.57
Peroxisomes 1.08 1.14 1.10 0.67 -3.64 -4.49 2.63 -3.57 -1.21 -4.21 0.45 1.38 0.84 1.62 -2.87
Vacuole 8.14 -2.12 -2.89 -26.94 0.52 -2.44 -7.39 -11.46 -11.36 -10.97 3.12 -7.62 -27.19 -28.49 -20.03
Vacuole Periphery 8.76 7.85 8.70 -1.45 4.28 1.10 3.02 -3.29 -3.56 -3.91 6.88 6.62 5.82 -2.80 -2.11
Standard name
Human Ortholog
Description Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Mrp8

Mrp8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available