Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 – – – 0.06 0.16 0.12 – – – 0.11 0 0.11 0.11 0.05 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 – – – 0 0 0 – – – 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Mitochondria – 0 – – – 0 0.05 0 – – – 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0.9 – – – 0.75 0.53 0.79 – – – 0.67 0.83 0.7 0.74 0.64 0.57 0.63 0.76 0.86 0.78 0.69 0.71 0.53
Nuclear Periphery – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.05 – – – 0.06 0.11 0 – – – 0.33 0.1 0.18 0.13 0.05 0.07 0.09 0.09 0 0.12 0.14 0.1 0.16
Peroxisomes – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.05 – – – 0.19 0.26 0.12 – – – 0 0.07 0.05 0.07 0.23 0.26 0.14 0.11 0.08 0.05 0.07 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – – – 0 0 0 – – – 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud – 0 – – – 0 0 0 – – – 0 0 0 0 1 0 0 0 0 0 1 0 1
Bud Neck – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 – – – 1 3 3 – – – 2 4 11 15 11 8 4 0 0 0 0 1 2
Endoplasmic Reticulum – 0 – – – 0 0 0 – – – 0 1 0 0 6 3 1 0 0 0 0 0 0
Endosome – 0 – – – 0 0 0 – – – 0 0 0 0 1 1 0 0 0 0 0 0 0
Golgi – 0 – – – 0 0 0 – – – 0 0 0 0 5 10 0 0 0 0 0 0 0
Mitochondria – 0 – – – 0 1 0 – – – 4 0 1 1 5 0 1 0 0 0 0 0 1
Nucleus – 19 – – – 12 10 19 – – – 12 87 67 100 138 74 27 37 53 31 49 77 54
Nuclear Periphery – 0 – – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus – 1 – – – 1 2 1 – – – 6 10 17 18 10 9 4 4 2 4 9 10 16
Peroxisomes – 0 – – – 0 0 0 – – – 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole – 0 – – – 0 0 0 – – – 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 1 – – – 3 5 3 – – – 0 7 5 10 50 34 6 5 5 2 5 17 20
Unique Cell Count 21 16 19 24 18 105 96 136 216 130 43 49 62 40 72 109 104
Labelled Cell Count 21 17 21 26 24 109 101 146 228 139 43 49 62 40 72 109 104


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 48.4 – – – 26.3 22.7 25.6 – – – 18.9 46.1 44.6 43.1 33.6 47.5 49.4 29.6 37.4 38.3
Std Deviation (1e-4) – 10.8 – – – 7.6 10.3 8.0 – – – 5.8 11.5 14.4 14.7 12.4 16.9 16.7 11.0 11.7 12.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.9703 46.7869 41.0066 31.0258 40.3171 40.5695 29.7117 39.8633 32.5575 31.191 36.5898 29.4346 39.1109 42.9788 44.8748 44.8103 43.038 43.1741
Actin 0.0013 0 0 0.0371 0 0 0.0009 0.0002 0.0008 0 0 0.0008 0.0647 0 0.0164 0 0 0.0082
Bud 0.0008 0 0 0.0002 0 0.0001 0.0024 0.0026 0.0001 0 0 0.0001 0.0008 0 0.0001 0 0 0.0003
Bud Neck 0.0003 0 0 0.0003 0 0.0002 0.0007 0.0001 0.0001 0 0 0.0004 0.0004 0 0 0 0 0.0004
Bud Periphery 0.0007 0 0 0.0001 0 0.0001 0.0014 0.0004 0.0001 0 0 0.0001 0.0009 0 0 0 0 0.0003
Bud Site 0.001 0 0 0.0005 0 0 0.0037 0.0003 0.0001 0 0 0.0001 0.0004 0 0.0001 0 0 0.0002
Cell Periphery 0.0014 0 0 0.0001 0 0.0002 0.0006 0.0001 0 0 0 0.0001 0.0001 0 0 0 0 0.0001
Cytoplasm 0.0043 0.0001 0 0.0235 0.0001 0.0001 0.0038 0.1268 0.0005 0.0002 0 0.0002 0.0101 0.0001 0.0039 0.0001 0.0001 0.0221
Cytoplasmic Foci 0.0014 0 0 0.0017 0 0 0.0012 0.0005 0.0001 0 0 0.0001 0.0027 0 0.0009 0 0 0.0053
Eisosomes 0.0003 0 0 0.0003 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0004 0 0 0.0134 0 0 0.0012 0.0001 0.0002 0 0 0.0001 0.0178 0 0.0005 0 0 0.0018
Endosome 0.0003 0 0 0.0129 0 0 0.0043 0.0001 0.0005 0 0 0.0002 0.0389 0 0.002 0 0 0.007
Golgi 0.0001 0 0 0.0016 0 0 0.0003 0 0.0001 0 0 0.0003 0.0079 0 0.006 0 0 0.0029
Lipid Particles 0.0011 0 0 0.0006 0 0 0.0006 0 0 0 0 0.0013 0.0013 0 0 0 0 0.0006
Mitochondria 0.0119 0.0006 0.0004 0.005 0.0019 0.0011 0.0052 0.0015 0.0036 0.0003 0.0009 0.0014 0.0067 0.0005 0.0016 0.0004 0.0013 0.0014
None 0.0019 0 0 0.0136 0 0 0.0019 0.0006 0.0001 0 0 0.0001 0.0028 0 0.0003 0 0 0.0034
Nuclear Periphery 0.0315 0.0027 0.0008 0.0628 0.001 0.0008 0.0406 0.0022 0.0231 0.0016 0.0006 0.0437 0.0769 0.002 0.0042 0.0018 0.0029 0.0038
Nucleolus 0.1806 0.014 0.0207 0.0113 0.1682 0.0584 0.0465 0.0049 0.0119 0.01 0.0409 0.0463 0.0044 0.007 0.0105 0.0028 0.0583 0.0392
Nucleus 0.7391 0.9822 0.9779 0.8082 0.8282 0.9377 0.8656 0.8569 0.9563 0.9874 0.9571 0.8838 0.7542 0.9901 0.9525 0.9948 0.9367 0.8899
Peroxisomes 0.0002 0 0 0.0004 0 0 0.0002 0 0 0 0 0 0.0006 0 0.0001 0 0 0.0005
Punctate Nuclear 0.0068 0 0 0.0039 0.0001 0 0.0129 0.0004 0.0013 0.0004 0 0.0199 0.0045 0 0.0005 0 0.0001 0.0116
Vacuole 0.0039 0.0001 0 0.0008 0.0001 0.0006 0.004 0.0015 0.0003 0 0.0001 0.0002 0.0013 0 0.0001 0 0.0001 0.0004
Vacuole Periphery 0.0106 0.0002 0.0001 0.0019 0.0003 0.0008 0.002 0.0009 0.0007 0.0001 0.0002 0.0007 0.0024 0.0001 0.0002 0.0001 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 137.1397 106.0846 142.3204 152.7919 122.784 114.678 147.3965 178.8292 193.4518 141.4752
Translational Efficiency 1.9859 2.3874 1.8872 1.9939 1.8761 2.6348 1.9226 1.5471 1.5045 1.7301

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1905 1214 1269 1204 1894 817 1650 39 3799 2031 2919 1243

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1865.42 2730.12 3385.83 3157.60 2549.12 2284.34 3270.65 3140.68 2206.28 2550.80 3320.72 3157.07
Standard Deviation 396.14 661.23 694.67 813.50 560.75 670.98 744.64 738.01 593.56 700.17 725.59 811.25
Intensity Change Log 2 — 0.549464 0.860009 0.759328 — -0.158222 0.359578 0.301078 — 0.183831 0.592496 0.512695

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000525 0.000621 0.000790 0.001076 0.000610 0.000637 0.000661 0.000483 0.000567 0.000627 0.000717 0.001057
Bud Neck 0.010119 0.005411 0.010509 0.029682 0.007298 0.004079 0.016654 0.012638 0.008713 0.004875 0.013983 0.029147
Bud Site 0.000489 0.000353 0.000506 0.007173 0.000315 0.000458 0.002201 0.001462 0.000402 0.000395 0.001464 0.006994
Cell Periphery 0.000149 0.000076 0.000039 0.000461 0.000098 0.000102 0.000173 0.000081 0.000124 0.000086 0.000115 0.000449
Cytoplasm 0.000374 0.000801 0.000092 0.011042 0.000263 0.001630 0.001713 0.001362 0.000318 0.001135 0.001008 0.010738
Cytoplasmic Foci 0.000046 0.000149 0.000291 0.005054 0.000062 0.000703 0.000889 0.004845 0.000054 0.000372 0.000629 0.005048
Eisosomes 0.000043 0.000064 0.000071 0.000028 0.000076 0.000040 0.000045 0.000063 0.000060 0.000054 0.000056 0.000030
Endoplasmic Reticulum 0.002104 0.000439 0.000271 0.002154 0.000836 0.000566 0.000568 0.000280 0.001472 0.000490 0.000439 0.002096
Endosome 0.000060 0.000024 0.000009 0.004425 0.000014 0.000177 0.000366 0.016900 0.000037 0.000085 0.000211 0.004817
Golgi 0.000023 0.000079 0.000039 0.003535 0.000018 0.000274 0.000233 0.003722 0.000021 0.000157 0.000148 0.003541
Lipid Particles 0.000005 0.000029 0.000005 0.000824 0.000003 0.000097 0.000052 0.000633 0.000004 0.000056 0.000032 0.000818
Mitochondria 0.001035 0.001395 0.002342 0.004578 0.001326 0.001617 0.002272 0.005998 0.001180 0.001484 0.002303 0.004623
Mitotic Spindle 0.000025 0.000629 0.000016 0.031306 0.000004 0.000546 0.006172 0.052605 0.000014 0.000596 0.003496 0.031975
None 0.001379 0.001302 0.000498 0.005246 0.001111 0.002234 0.002811 0.000861 0.001246 0.001677 0.001805 0.005108
Nuclear Periphery 0.000323 0.000084 0.000165 0.003237 0.000113 0.000097 0.000398 0.000383 0.000218 0.000089 0.000296 0.003148
Nuclear Periphery Foci 0.000036 0.000082 0.000010 0.002166 0.000007 0.000412 0.000061 0.002189 0.000022 0.000215 0.000039 0.002166
Nucleolus 0.017730 0.024775 0.040522 0.017913 0.028251 0.016244 0.015708 0.022449 0.022975 0.021343 0.026496 0.018056
Nucleus 0.964393* 0.961819* 0.940966* 0.864787* 0.958290* 0.968321* 0.947774* 0.847314* 0.961351* 0.964434* 0.944815* 0.864238*
Peroxisomes 0.000169 0.000963 0.002333 0.001088 0.000595 0.000667 0.000586 0.001350 0.000381 0.000844 0.001346 0.001096
Vacuole 0.000596 0.000382 0.000263 0.002708 0.000439 0.000808 0.000430 0.021003 0.000518 0.000553 0.000357 0.003282
Vacuole Periphery 0.000376 0.000526 0.000263 0.001516 0.000269 0.000292 0.000232 0.003377 0.000323 0.000432 0.000245 0.001574

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.80 -9.68 -8.87 -6.90 -3.86 -0.65 -5.75 -0.13 0.43 2.50 -1.88 -10.78 -8.42 -6.35 -4.34
Bud Neck 8.61 -1.77 -15.30 -17.97 -14.73 5.38 -15.20 -3.30 -4.39 2.55 9.79 -14.02 -16.38 -18.44 -10.81
Bud Site 2.25 -0.78 -6.29 -6.49 -6.24 -1.70 -6.65 -3.49 -3.03 1.12 0.04 -6.58 -6.47 -6.47 -4.71
Cell Periphery 1.76 2.78 -1.94 -2.79 -3.17 -0.31 -1.86 -1.16 -1.07 0.30 1.34 0.37 -2.29 -2.70 -2.43
Cytoplasm -1.07 2.99 -7.21 -6.58 -7.51 -2.42 -6.92 -2.69 -0.42 -0.91 -2.63 -5.37 -7.35 -6.13 -6.68
Cytoplasmic Foci -2.57 -6.23 -4.12 -3.96 -3.66 -1.42 -3.82 -1.67 -1.14 -1.11 -1.71 -4.84 -4.22 -3.55 -3.26
Eisosomes -4.46 -8.03 3.77 8.26 14.84 6.33 4.74 2.19 -1.50 -0.44 1.29 -0.16 8.34 8.17 13.72
Endoplasmic Reticulum 13.46 15.43 1.29 -1.51 -1.79 1.81 2.50 4.80 1.61 3.49 11.06 13.22 0.29 -1.40 -1.47
Endosome 4.46 8.63 -3.49 -3.55 -3.58 -1.36 -1.90 -1.01 -0.99 -0.98 -1.00 -1.54 -3.65 -3.55 -3.45
Golgi -1.34 -4.98 -3.19 -3.08 -3.15 -1.35 -3.43 -1.24 -1.02 -1.08 -1.66 -3.64 -3.29 -2.96 -3.02
Lipid Particles -1.24 -0.62 -1.95 -1.83 -1.95 -1.64 -2.98 -1.50 -0.99 -1.26 -2.02 -2.99 -2.01 -1.72 -1.86
Mitochondria -5.06 -14.84 -14.22 -12.77 -8.66 -0.83 -15.48 -1.61 -1.41 -0.85 -1.96 -21.61 -13.81 -10.95 -8.24
Mitotic Spindle -1.60 0.54 -8.08 -7.90 -8.09 -1.08 -4.53 -1.46 -1.44 -1.26 -1.89 -4.50 -8.21 -8.01 -7.14
None 0.13 1.75 -5.61 -6.47 -9.84 -1.50 -2.46 -1.04 0.53 1.38 -0.92 -1.27 -6.60 -6.09 -6.37
Nuclear Periphery 8.26 3.76 -7.83 -8.44 -8.19 0.04 -8.00 -2.12 -2.12 -0.80 7.11 -5.22 -8.15 -8.47 -7.57
Nuclear Periphery Foci -0.85 1.94 -3.88 -3.75 -3.95 -1.77 -4.29 -1.65 -0.87 -1.53 -1.95 -2.41 -4.01 -3.28 -3.92
Nucleolus -2.99 -7.82 -2.93 0.10 5.12 4.32 3.70 0.65 -0.57 -0.27 0.76 -3.38 -0.73 -1.25 1.96
Nucleus 0.84 7.35 15.55 14.59 10.77 -2.15 6.27 1.97 2.16 1.41 -0.99 9.65 15.39 15.33 11.17
Peroxisomes -6.03 -8.96 -5.37 0.81 5.79 -0.70 -1.32 -0.56 -0.31 -0.29 -4.78 -8.91 -3.68 0.22 3.84
Vacuole 2.41 3.71 -5.41 -6.11 -6.39 -1.27 -2.85 -1.23 -1.18 -1.19 -0.48 -1.03 -5.25 -4.85 -5.00
Vacuole Periphery -0.48 2.24 -2.82 -1.11 -3.47 -0.59 1.24 -0.93 -0.89 -0.96 -0.63 2.52 -3.29 -1.92 -3.74
Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpt1

Rpt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available