Standard name
Human Ortholog
Description RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies; human homolog DBR1 can complement yeast dbr1 null mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.05 0.11 0.18 0.12 0.15 0.14 0.15 0.14 0.1 0.08 0.07 0.27 0.32 0.33 0.16 0.12 0.14 0.05 0 0 0.11 0.12 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.55 0.18 0.11 0.27 0.3 0.7 0.76 0.66 0.74 0.83 0.85 0.83 0 0 0 0.09 0.08 0.07 0 0 0.05 0 0.06 0.06
Nucleus 0.6 0.63 0.69 0.6 0.55 0.35 0.41 0.4 0.43 0.39 0.44 0.38 0.64 0.58 0.52 0.58 0.69 0.55 0.69 0.75 0.58 0.52 0.39 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.27 0.16 0.08 0.17 0.14 0.09 0.09 0.07 0.09 0.08 0.11 0.11 0.14 0.2 0.24 0.21 0.29 0.21 0.16 0.27 0.28 0.34 0.38
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 2 0 14 3 8 4 3 3 0 0 0 1 0 2 0 1 3 2 6 3
Bud Neck 0 0 1 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 3 6
Bud Site 0 0 0 1 0 3 6 5 4 9 8 4 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 4 25 31 25 39 53 55 48 45 23 22 81 112 149 37 21 32 4 3 12 24 42 50
Endoplasmic Reticulum 1 1 1 0 0 0 0 0 0 0 0 0 1 2 1 8 9 15 0 0 2 1 1 0
Endosome 0 1 3 2 3 0 0 0 0 0 0 0 2 3 11 7 2 11 0 1 1 1 4 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 0 1 0
Mitochondria 56 15 25 47 61 178 291 244 251 374 233 280 1 1 3 21 14 16 1 0 14 6 20 22
Nucleus 61 53 153 103 110 89 158 148 145 177 122 130 195 200 236 138 118 124 70 80 163 114 135 121
Nuclear Periphery 1 0 0 0 1 1 0 0 2 3 1 6 0 0 0 2 0 0 0 0 1 0 0 2
Nucleolus 1 0 0 0 0 0 0 1 0 2 2 2 0 0 0 1 1 1 0 0 0 0 2 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 2 0 1 1 0 0 1 1 1 3 2 2 6 4 0 1 1 0 2 4 9 8
Vac/Vac Membrane 15 23 36 13 34 36 33 33 24 41 23 38 34 49 89 56 35 65 21 17 74 61 117 143
Unique Cell Count 102 84 223 173 201 255 381 368 338 450 275 338 303 347 452 236 170 224 102 107 282 222 349 375
Labelled Cell Count 144 97 246 198 237 347 555 490 484 656 416 488 317 369 497 275 201 270 102 107 282 222 349 375


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.9 4.6 4.6 4.5 3.4 3.4 3.5 3.3 3.1 3.1 3.0 5.8 5.7 5.5 6.4 5.8 6.3 6.0 6.1 6.2
Std Deviation (1e-4) 1.0 0.7 1.3 2.1 2.0 0.9 1.0 1.3 1.1 0.7 1.5 0.8 1.1 1.4 1.2 1.5 1.2 1.8 1.4 1.2 1.3
Intensity Change (Log2) – – – -0.02 -0.06 -0.46 -0.47 -0.42 -0.48 -0.57 -0.6 -0.61 0.33 0.3 0.25 0.46 0.32 0.44 0.37 0.38 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0.4 1.3 1.0 1.3 1.0 -0.5 -1.1 -2.0 4.4 5.7 6.1 1.4 0.3 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.7 -6.1 -6.5 -0.8 -1.0 -1.5
Nucleus -1.9 -2.9 -7.4 -6.4 -6.7 -6.0 -7.2 -5.4 -7.0 -1.0 -2.6 -4.1 -2.3 0.2 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 0.2 -0.6 -2.8 -2.6 -3.4 -2.7 -2.7 -1.7 -1.6 -0.7 1.1 2.0 1.1 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3696 0.0942 -0.3033 0.2121 -1.3698 -0.2601 0.5656 0.4153 0.0329 0.0725 1.1044 0.07 -0.7547 -0.5679 -0.8658 -0.7318 -0.6122 -0.8623
Actin 0.005 0.0001 0.0093 0.0292 0.0002 0.0005 0.0723 0.0003 0.0068 0.0067 0.0226 0.0014 0.0192 0.0002 0.0156 0.0009 0.0587 0.0067
Bud 0.0001 0.0141 0.0009 0.0003 0 0.0001 0.0009 0.0002 0.0007 0.0004 0.001 0.0002 0.0004 0.0002 0.0004 0.0002 0.0002 0.0042
Bud Neck 0.0009 0.0003 0.001 0.0016 0.0012 0.0027 0.0034 0.0008 0.001 0.0014 0.0135 0.0034 0.0006 0.0003 0.0047 0.0004 0.0013 0.0067
Bud Periphery 0.0002 0.0026 0.0029 0.0003 0 0.0001 0.0013 0.0002 0.0013 0.0007 0.0009 0.0004 0.0006 0.0001 0.0005 0.0002 0.0002 0.0018
Bud Site 0.001 0.0062 0.0006 0.001 0 0.0001 0.0094 0.0013 0.0062 0.0016 0.0071 0.0004 0.0009 0.0007 0.0017 0.0005 0.0007 0.0028
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0 0 0.0008 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.1344 0.1032 0.1371 0.0975 0.1892 0.0929 0.0884 0.1578 0.1308 0.1261 0.0988 0.1015 0.1148 0.1056 0.0867 0.1233 0.1037 0.1015
Cytoplasmic Foci 0.0054 0.0049 0.0097 0.0309 0.0022 0.0052 0.0251 0.01 0.0119 0.0184 0.0314 0.0058 0.0298 0.0041 0.008 0.0146 0.0026 0.0056
Eisosomes 0.0003 0.0001 0.0003 0.0002 0 0.0001 0.0007 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.009 0.0028 0.0064 0.0029 0.0014 0.0018 0.0096 0.0037 0.0043 0.0031 0.0031 0.0023 0.0068 0.0048 0.0032 0.0013 0.0011 0.0038
Endosome 0.009 0.0031 0.0065 0.0309 0.0015 0.003 0.0363 0.0024 0.0156 0.0266 0.0193 0.0086 0.016 0.0027 0.0118 0.0024 0.0028 0.0084
Golgi 0.0007 0.0001 0.0003 0.0315 0 0.0001 0.0139 0.0001 0.003 0.0158 0.0104 0.0036 0.0025 0 0.0046 0.0005 0.0046 0.003
Lipid Particles 0.0011 0.0004 0.0009 0.0105 0.0001 0.0004 0.0185 0.0003 0.0027 0.0157 0.0059 0.01 0.0089 0.0007 0.0033 0.0093 0.0026 0.0012
Mitochondria 0.0011 0.0004 0.002 0.0047 0.0007 0.0007 0.0113 0.0008 0.0035 0.0358 0.0028 0.0047 0.0016 0.0005 0.0022 0.0251 0.0521 0.0016
None 0.3403 0.3136 0.2654 0.1458 0.34 0.2644 0.2009 0.2467 0.1805 0.2575 0.1111 0.0688 0.1242 0.19 0.1552 0.1053 0.2431 0.085
Nuclear Periphery 0.0246 0.0239 0.0442 0.0173 0.0106 0.0109 0.0326 0.0145 0.0288 0.02 0.017 0.0141 0.0418 0.0258 0.0249 0.0059 0.0061 0.0196
Nucleolus 0.0066 0.0036 0.0073 0.0086 0.0052 0.0058 0.0028 0.0042 0.0035 0.0021 0.0154 0.012 0.0037 0.004 0.009 0.0045 0.0041 0.0065
Nucleus 0.4274 0.4936 0.4697 0.5018 0.4022 0.5713 0.4211 0.5327 0.5684 0.4295 0.579 0.7198 0.5908 0.6298 0.6153 0.6919 0.4975 0.711
Peroxisomes 0.0002 0.0001 0.0005 0.0084 0.0001 0.0001 0.0161 0.0004 0.0024 0.0096 0.0071 0.0017 0.0069 0 0.0052 0.0035 0.0019 0.0007
Punctate Nuclear 0.0257 0.024 0.0297 0.0743 0.0447 0.0381 0.0252 0.0209 0.0222 0.0217 0.0362 0.0381 0.0252 0.0255 0.0437 0.0083 0.0155 0.0257
Vacuole 0.0057 0.0022 0.0044 0.0014 0.0005 0.0014 0.007 0.0024 0.005 0.0052 0.0141 0.0024 0.0038 0.0041 0.0029 0.001 0.0008 0.0032
Vacuole Periphery 0.001 0.0004 0.001 0.0008 0.0002 0.0004 0.0024 0.0004 0.0015 0.0017 0.0031 0.0007 0.0015 0.0007 0.0009 0.0007 0.0004 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0591 5.6688 7.8121 7.447 9.9837 12.6758 10.2353 8.2897 7.0677 9.9697
Translational Efficiency 0.9081 0.9849 0.7861 0.9415 0.6918 0.5871 0.6797 0.5938 0.8091 0.6703

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies; human homolog DBR1 can complement yeast dbr1 null mutant
Localization
Cell Percentages cytoplasm (20%), nucleus (18%), mixed (59%)
Cell Cycle Regulation Yes
nucleus - T/G1 (0.00088)
Subcompartmental Group N/A

Dbr1

Dbr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available