ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.05 0.05 0.05 0.06 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.48 0.4 0.63 0.54 0.32 0.27 0.18 0.17 0.12 0.14 0.12 0.1 0.82 0.88 0.85 0.5 0.55 0.64 0.15 0.14 0.08 0.18 0.29 0.27
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0.09 0.07 0.1 0 0 0 0 0 0 0 0 0.06 0.07 0.23 0.18 0.13 0.1 0.06 0.11 0.06 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.66 0.55 0.11 0.42 0.52 0.71 0.81 0.76 0.87 0.9 0.88 0.92 0 0 0 0.07 0.09 0.1 0.57 0.64 0.61 0.56 0.43 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.13 0.06 0.09 0.07 0.08 0.1 0.1 0.05 0.06 0.07 0.05 0 0 0.05 0.07 0.06 0 0 0 0 0.05 0.06
Vac/Vac Membrane 0 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.15 0.11 0.08 0.05 0.06 0 0.06 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 3 6 3 2 6 3 2 1 8 2 4 7
Bud 4 0 7 5 12 16 23 23 27 29 30 31 2 1 3 2 1 2 0 0 1 0 3 9
Bud Neck 0 0 4 0 0 0 1 1 0 3 0 0 0 4 1 0 0 0 0 1 2 0 4 6
Bud Site 0 0 0 0 0 3 12 2 5 8 11 10 0 0 0 0 2 0 – – – – – –
Cell Periphery 0 1 3 5 0 2 0 1 0 1 3 3 2 0 1 5 5 3 0 0 1 0 1 0
Cytoplasm 60 35 137 139 73 83 77 80 56 67 53 50 136 213 201 66 63 68 22 16 24 22 70 74
Endoplasmic Reticulum 4 11 3 0 0 1 0 1 0 1 0 2 1 1 6 6 6 9 1 0 5 1 0 2
Endosome 0 0 19 19 23 10 4 19 6 0 8 1 6 15 17 30 21 14 15 7 31 7 10 15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 1 3 0 0 3
Mitochondria 83 48 23 107 117 219 352 351 398 436 393 476 4 3 2 9 10 11 83 73 176 68 102 100
Nucleus 0 1 0 2 3 2 10 5 8 6 4 6 0 1 0 0 1 0 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 1 0 3 2 1 0 3 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 1 3 1 2 2 0 3 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 10 11 4 2 8 9 7 2 3 2 0 0 1 0 1 1 1 0 4 0 0 0
SpindlePole 0 0 28 16 21 22 35 46 44 25 25 36 8 4 8 7 8 6 5 3 9 4 12 15
Vac/Vac Membrane 0 5 7 6 8 17 17 19 13 17 15 20 7 7 6 20 13 8 6 7 5 7 17 22
Unique Cell Count 126 88 217 256 225 307 436 464 459 483 446 517 165 243 237 131 115 106 145 115 288 122 239 273
Labelled Cell Count 151 101 241 310 261 379 542 561 568 598 545 643 169 255 250 150 139 125 145 115 288 122 239 273


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.1 4.6 3.9 4.1 3.7 3.4 3.5 3.4 3.1 3.0 3.0 5.3 5.4 5.2 6.9 6.8 6.4 5.0 5.3 6.1
Std Deviation (1e-4) 0.3 0.6 1.7 1.5 1.5 2.0 1.4 1.3 1.4 1.1 1.3 0.8 1.1 1.1 1.2 1.7 1.8 1.4 1.4 1.1 2.0
Intensity Change (Log2) – – – -0.21 -0.17 -0.31 -0.43 -0.38 -0.44 -0.55 -0.6 -0.59 0.21 0.24 0.2 0.6 0.58 0.49 0.14 0.22 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 4.1 2.9 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2755 0.7182 0.5653 0.5058 0.0403 1.0737 -0.0773 0.2364 0.1106 -0.295 -0.0417 0.2402 -0.9693 -0.4002 -0.8357 -0.6375 -1.0868 -0.6829
Actin 0.0068 0.0058 0.0114 0.0036 0.0024 0.0127 0.0489 0.0134 0.0173 0.0165 0.0058 0.0091 0.0227 0.0048 0.0072 0.0047 0.0038 0.0054
Bud 0.003 0.0048 0.0033 0.0032 0.0009 0.002 0.0018 0.0098 0.0018 0.0161 0.0097 0.0021 0.0047 0.0131 0.0056 0.0024 0.0068 0.0028
Bud Neck 0.0051 0.0033 0.0029 0.0015 0.0011 0.0087 0.0045 0.0017 0.016 0.0025 0.001 0.0231 0.0092 0.0012 0.0025 0.0007 0.0007 0.0036
Bud Periphery 0.0036 0.008 0.0082 0.0114 0.002 0.0035 0.006 0.0064 0.0024 0.0271 0.0109 0.0032 0.0036 0.0101 0.0074 0.0059 0.0415 0.0024
Bud Site 0.0153 0.0227 0.0117 0.0025 0.0014 0.002 0.0225 0.0577 0.0178 0.006 0.0143 0.0036 0.0088 0.0393 0.0129 0.0018 0.0017 0.0012
Cell Periphery 0.0036 0.0008 0.0024 0.002 0.0003 0.0005 0.0014 0.0015 0.0008 0.0022 0.0002 0.0008 0.0007 0.0019 0.0007 0.0006 0.0008 0.0004
Cytoplasm 0.0555 0.1035 0.0795 0.0969 0.0721 0.0733 0.1233 0.1943 0.1844 0.1119 0.0733 0.1312 0.0607 0.1297 0.1147 0.1049 0.074 0.1195
Cytoplasmic Foci 0.1811 0.1852 0.1679 0.1504 0.2363 0.1599 0.1462 0.1268 0.132 0.1166 0.14 0.1627 0.1509 0.1571 0.1347 0.0826 0.1576 0.1432
Eisosomes 0.0007 0.0003 0.0009 0.001 0.0001 0.0003 0.002 0.0004 0.0004 0.0003 0.0001 0.0001 0.0005 0.0002 0.0004 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0099 0.0023 0.0047 0.0041 0.0018 0.004 0.0134 0.0076 0.0066 0.0088 0.0051 0.0054 0.0072 0.0083 0.0075 0.0049 0.0079 0.0073
Endosome 0.227 0.1939 0.1397 0.1207 0.2156 0.1582 0.2257 0.2065 0.2545 0.2283 0.3814 0.2288 0.2819 0.2681 0.2526 0.2489 0.2485 0.2276
Golgi 0.0645 0.0679 0.0507 0.0429 0.0567 0.0605 0.0529 0.0704 0.0696 0.0604 0.0796 0.0853 0.0871 0.0815 0.0645 0.0583 0.1012 0.0813
Lipid Particles 0.0437 0.013 0.0189 0.0144 0.031 0.0171 0.0385 0.0119 0.0162 0.0146 0.0183 0.0164 0.0466 0.0088 0.0096 0.0034 0.0178 0.0195
Mitochondria 0.1877 0.2021 0.3123 0.3883 0.2498 0.2926 0.0988 0.1431 0.1542 0.3016 0.0645 0.226 0.1807 0.1337 0.2255 0.3414 0.2266 0.2764
None 0.1254 0.1103 0.1105 0.0964 0.0149 0.0967 0.1423 0.0871 0.0705 0.021 0.1235 0.0368 0.06 0.0903 0.0917 0.0857 0.0185 0.0378
Nuclear Periphery 0.0035 0.0006 0.0017 0.0013 0.0005 0.0009 0.0116 0.0034 0.0035 0.0026 0.0052 0.0057 0.0062 0.0028 0.0026 0.0018 0.0146 0.0041
Nucleolus 0.0042 0.0006 0.001 0.0008 0.0004 0.0013 0.0012 0.001 0.0036 0.0006 0.0006 0.0009 0.0052 0.0018 0.0008 0.0006 0.0009 0.0014
Nucleus 0.0035 0.0009 0.0016 0.0015 0.0009 0.005 0.0039 0.0041 0.0023 0.0018 0.0028 0.0038 0.005 0.0031 0.0021 0.0013 0.006 0.0056
Peroxisomes 0.0366 0.0618 0.0583 0.0463 0.101 0.0886 0.0171 0.0207 0.0192 0.0166 0.0427 0.0251 0.0291 0.0191 0.0312 0.0096 0.0262 0.0325
Punctate Nuclear 0.0036 0.001 0.0014 0.001 0.0013 0.0014 0.0118 0.0021 0.0026 0.0009 0.0027 0.0045 0.0042 0.0023 0.0013 0.0005 0.0047 0.0031
Vacuole 0.0116 0.008 0.0078 0.0063 0.0064 0.0075 0.0203 0.025 0.0178 0.0251 0.0104 0.0154 0.0174 0.0184 0.016 0.0277 0.0253 0.0169
Vacuole Periphery 0.004 0.0031 0.0035 0.0033 0.0032 0.0035 0.0061 0.0052 0.0066 0.0183 0.008 0.0102 0.0074 0.0045 0.0084 0.0119 0.0147 0.008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3886 5.711 3.6324 6.1906 5.9165 8.2094 4.3308 7.5163 5.2313 5.2888
Translational Efficiency 0.4325 0.6268 0.7759 0.4774 0.4389 0.5539 1.0301 0.7799 1.1015 0.9041

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
239 218 159 1629 1479 1561 129 1220 1718 1779 288 2849

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 644.23 781.74 921.55 863.13 674.44 712.95 970.71 864.61 670.24 721.38 943.57 863.76
Standard Deviation 75.65 94.00 83.58 112.08 88.60 85.37 94.87 134.63 87.54 89.36 92.12 122.25
Intensity Change Log 2 — 0.279113 0.516487 0.422002 — 0.080111 0.525350 0.358359 — 0.180763 0.521027 0.389803

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000624 0.001927 0.000662 0.034771 0.000174 0.000544 0.000879 0.028530 0.000237 0.000714 0.000759 0.032098
Bud Neck 0.042424 0.068145 0.003959 0.017068 0.022135 0.067117 0.009679 0.014674 0.024958 0.067243 0.006521 0.016043
Bud Site 0.012673 0.032963 0.003806 0.123321* 0.007447 0.018097 0.020430 0.167932* 0.008174 0.019919 0.011252 0.142424*
Cell Periphery 0.000185 0.000284 0.000309 0.000452 0.000172 0.000322 0.000384 0.000431 0.000173 0.000317 0.000343 0.000443
Cytoplasm 0.201909* 0.125306* 0.244358* 0.052664 0.431859* 0.131422* 0.266490* 0.084823 0.399869* 0.130672* 0.254271* 0.066435
Cytoplasmic Foci 0.408155* 0.306060* 0.067698 0.133549 0.290424* 0.262172* 0.164229 0.146797 0.306802* 0.267550* 0.110936 0.139222
Eisosomes 0.000280 0.000250 0.000161 0.000225 0.000132 0.000258 0.000220 0.000194 0.000152 0.000257 0.000187 0.000211
Endoplasmic Reticulum 0.000756 0.001121 0.008933 0.004640 0.001443 0.001513 0.005440 0.003612 0.001348 0.001465 0.007368 0.004199
Endosome 0.056851 0.095548 0.021092 0.092035 0.021747 0.078159 0.044771 0.063387 0.026631 0.080290 0.031698 0.079767
Golgi 0.007175 0.023368 0.000403 0.072931 0.005830 0.034643 0.001044 0.081597 0.006017 0.033261 0.000690 0.076642
Lipid Particles 0.022033 0.011341 0.012315 0.004095 0.014682 0.028582 0.015828 0.002321 0.015705 0.026469 0.013889 0.003335
Mitochondria 0.026335 0.067128 0.000597 0.138684* 0.017617 0.140619* 0.001622 0.112215* 0.018830 0.131613* 0.001056 0.127349*
Mitotic Spindle 0.002573 0.014219 0.001613 0.098458 0.000937 0.009581 0.006990 0.096899 0.001164 0.010149 0.004021 0.097790
None 0.001463 0.000744 0.002248 0.000263 0.005368 0.002167 0.003531 0.000383 0.004825 0.001993 0.002823 0.000315
Nuclear Periphery 0.000558 0.000437 0.003907 0.000704 0.000675 0.001054 0.002376 0.000251 0.000659 0.000978 0.003221 0.000510
Nuclear Periphery Foci 0.000357 0.000553 0.006640 0.001445 0.000489 0.000506 0.006066 0.000595 0.000470 0.000512 0.006383 0.001081
Nucleolus 0.002739 0.001923 0.003017 0.000826 0.002617 0.003949 0.006216 0.000426 0.002634 0.003700 0.004450 0.000655
Nucleus 0.102569 0.059667 0.458360* 0.008072 0.124843 0.072241 0.266901* 0.009189 0.121744 0.070700 0.372602* 0.008551
Peroxisomes 0.060186 0.117200* 0.003668 0.092719 0.009613 0.046127 0.012129 0.111944* 0.016649 0.054837 0.007458 0.100952*
Vacuole 0.047742 0.067758 0.155392 0.106617 0.038541 0.090597 0.163582 0.065353 0.039821 0.087798 0.159060 0.088947
Vacuole Periphery 0.002414 0.004059 0.000863 0.016460 0.003254 0.010333 0.001193 0.008447 0.003137 0.009564 0.001011 0.013029

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.29 -0.64 -12.10 -11.41 -12.07 -4.47 -6.18 -11.82 -11.66 -11.47 -4.70 -7.51 -16.72 -16.44 -16.39
Bud Neck -2.27 6.40 4.15 5.16 -11.47 -11.48 4.48 3.76 14.04 -2.25 -11.37 9.30 4.77 14.78 -7.43
Bud Site -3.75 2.86 -21.20 -13.88 -26.48* -6.61 -1.51 -27.15* -24.80 -16.30 -7.48 -0.78 -36.55 -31.68 -27.25*
Cell Periphery -1.73 -3.82 -6.07 -2.40 -2.97 -3.82 -5.66 -6.70 -1.82 -0.96 -4.08 -7.55 -9.70 -2.55 -2.81
Cytoplasm 3.90 -1.11 9.67 5.47 10.15 35.27* 8.91 41.26 6.55 8.78 33.48 11.44 46.04 11.88 13.46
Cytoplasmic Foci 4.72 20.82 17.72 11.07 -9.14 3.47 8.80 20.66 17.46 0.13 5.13 20.99 27.89 22.74 -4.44
Eisosomes 0.88 4.02 2.19 1.07 -2.98 -6.33 -4.09 -5.80 3.06 1.13 -5.76 -2.77 -5.69 2.59 -1.32
Endoplasmic Reticulum -1.71 -10.14 -5.04 -4.44 4.69 -0.27 -5.94 -3.61 -3.54 2.52 -0.55 -10.93 -5.49 -5.30 5.20
Endosome -2.83 4.80 -3.95 0.49 -16.40 -17.20 -1.99 -12.34 4.24 -2.78 -16.03 -0.20 -18.04 0.92 -11.03
Golgi -5.26 6.12 -18.47 -10.78 -21.70* -13.70 9.16 -19.40 -10.21 -20.90* -14.44 11.46 -27.32 -13.02 -30.07*
Lipid Particles 2.88 3.31 5.79 3.33 4.14 -7.09 0.36 11.27 15.55 5.58 -6.02 2.20 11.71 15.20 6.85
Mitochondria -3.34 4.12 -12.69 -5.52 -23.48* -21.63* 9.58 -15.11 4.30 -18.53* -21.50 10.75 -23.52 1.73 -29.86*
Mitotic Spindle -3.14 -0.10 -19.11 -14.17 -18.74 -6.09 -1.87 -17.42 -15.32 -12.82 -6.76 -2.11 -26.32 -22.55 -22.19
None 1.46 -1.37 2.65 2.32 6.64 4.33 1.78 7.59 5.07 2.17 4.40 2.90 7.97 5.53 3.99
Nuclear Periphery 0.71 -6.65 -1.45 -2.05 6.20 -1.77 -4.65 3.45 3.71 5.65 -1.71 -7.87 0.79 2.17 8.12
Nuclear Periphery Foci -1.15 -5.36 -4.55 -3.37 4.16 -0.08 -6.56 -2.60 -1.22 6.23 -0.23 -7.94 -4.75 -3.27 6.74
Nucleolus 1.18 0.36 3.57 2.08 3.87 -2.60 -1.57 8.85 7.85 2.79 -2.32 -1.37 8.74 7.59 3.71
Nucleus 2.81 -14.06 9.47 5.05 19.24 9.89 -6.57 27.82 15.26 11.87 10.17 -14.47 30.39 16.75 21.36
Peroxisomes -3.80 7.55 -3.50 1.96 -21.06* -14.15 -0.58 -19.53 -11.20 -17.68* -12.68 5.63 -23.79 -10.40 -27.16*
Vacuole -2.16 -8.57 -10.39 -5.85 3.94 -12.43 -8.46 -9.39 1.84 6.17 -12.42 -12.72 -18.07 -3.62 7.08
Vacuole Periphery -2.43 4.12 -10.75 -8.95 -12.69 -9.17 7.11 -6.62 2.27 -10.33 -9.41 8.57 -11.97 -2.90 -15.71
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
Localization
Cell Percentages cytoplasm (27%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKL162C

YKL162C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl162c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available