ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 3 | 2 | 6 | 3 | 2 | 1 | 8 | 2 | 4 | 7 |
Bud | 4 | 0 | 7 | 5 | 12 | 16 | 23 | 23 | 27 | 29 | 30 | 31 | 2 | 1 | 3 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 9 |
Bud Neck | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 4 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 12 | 2 | 5 | 8 | 11 | 10 | 0 | 0 | 0 | 0 | 2 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 3 | 5 | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 3 | 2 | 0 | 1 | 5 | 5 | 3 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 60 | 35 | 137 | 139 | 73 | 83 | 77 | 80 | 56 | 67 | 53 | 50 | 136 | 213 | 201 | 66 | 63 | 68 | 22 | 16 | 24 | 22 | 70 | 74 |
Endoplasmic Reticulum | 4 | 11 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 6 | 6 | 6 | 9 | 1 | 0 | 5 | 1 | 0 | 2 |
Endosome | 0 | 0 | 19 | 19 | 23 | 10 | 4 | 19 | 6 | 0 | 8 | 1 | 6 | 15 | 17 | 30 | 21 | 14 | 15 | 7 | 31 | 7 | 10 | 15 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 3 | 0 | 0 | 3 |
Mitochondria | 83 | 48 | 23 | 107 | 117 | 219 | 352 | 351 | 398 | 436 | 393 | 476 | 4 | 3 | 2 | 9 | 10 | 11 | 83 | 73 | 176 | 68 | 102 | 100 |
Nucleus | 0 | 1 | 0 | 2 | 3 | 2 | 10 | 5 | 8 | 6 | 4 | 6 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 2 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 10 | 11 | 4 | 2 | 8 | 9 | 7 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 4 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 28 | 16 | 21 | 22 | 35 | 46 | 44 | 25 | 25 | 36 | 8 | 4 | 8 | 7 | 8 | 6 | 5 | 3 | 9 | 4 | 12 | 15 |
Vac/Vac Membrane | 0 | 5 | 7 | 6 | 8 | 17 | 17 | 19 | 13 | 17 | 15 | 20 | 7 | 7 | 6 | 20 | 13 | 8 | 6 | 7 | 5 | 7 | 17 | 22 |
Unique Cell Count | 126 | 88 | 217 | 256 | 225 | 307 | 436 | 464 | 459 | 483 | 446 | 517 | 165 | 243 | 237 | 131 | 115 | 106 | 145 | 115 | 288 | 122 | 239 | 273 |
Labelled Cell Count | 151 | 101 | 241 | 310 | 261 | 379 | 542 | 561 | 568 | 598 | 545 | 643 | 169 | 255 | 250 | 150 | 139 | 125 | 145 | 115 | 288 | 122 | 239 | 273 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 4.1 | 4.6 | 3.9 | 4.1 | 3.7 | 3.4 | 3.5 | 3.4 | 3.1 | 3.0 | 3.0 | 5.3 | 5.4 | 5.2 | 6.9 | 6.8 | 6.4 | 5.0 | 5.3 | 6.1 |
Std Deviation (1e-4) | 0.3 | 0.6 | 1.7 | 1.5 | 1.5 | 2.0 | 1.4 | 1.3 | 1.4 | 1.1 | 1.3 | 0.8 | 1.1 | 1.1 | 1.2 | 1.7 | 1.8 | 1.4 | 1.4 | 1.1 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.17 | -0.31 | -0.43 | -0.38 | -0.44 | -0.55 | -0.6 | -0.59 | 0.21 | 0.24 | 0.2 | 0.6 | 0.58 | 0.49 | 0.14 | 0.22 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.1 | 2.9 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2755 | 0.7182 | 0.5653 | 0.5058 | 0.0403 | 1.0737 | -0.0773 | 0.2364 | 0.1106 | -0.295 | -0.0417 | 0.2402 | -0.9693 | -0.4002 | -0.8357 | -0.6375 | -1.0868 | -0.6829 |
Actin | 0.0068 | 0.0058 | 0.0114 | 0.0036 | 0.0024 | 0.0127 | 0.0489 | 0.0134 | 0.0173 | 0.0165 | 0.0058 | 0.0091 | 0.0227 | 0.0048 | 0.0072 | 0.0047 | 0.0038 | 0.0054 |
Bud | 0.003 | 0.0048 | 0.0033 | 0.0032 | 0.0009 | 0.002 | 0.0018 | 0.0098 | 0.0018 | 0.0161 | 0.0097 | 0.0021 | 0.0047 | 0.0131 | 0.0056 | 0.0024 | 0.0068 | 0.0028 |
Bud Neck | 0.0051 | 0.0033 | 0.0029 | 0.0015 | 0.0011 | 0.0087 | 0.0045 | 0.0017 | 0.016 | 0.0025 | 0.001 | 0.0231 | 0.0092 | 0.0012 | 0.0025 | 0.0007 | 0.0007 | 0.0036 |
Bud Periphery | 0.0036 | 0.008 | 0.0082 | 0.0114 | 0.002 | 0.0035 | 0.006 | 0.0064 | 0.0024 | 0.0271 | 0.0109 | 0.0032 | 0.0036 | 0.0101 | 0.0074 | 0.0059 | 0.0415 | 0.0024 |
Bud Site | 0.0153 | 0.0227 | 0.0117 | 0.0025 | 0.0014 | 0.002 | 0.0225 | 0.0577 | 0.0178 | 0.006 | 0.0143 | 0.0036 | 0.0088 | 0.0393 | 0.0129 | 0.0018 | 0.0017 | 0.0012 |
Cell Periphery | 0.0036 | 0.0008 | 0.0024 | 0.002 | 0.0003 | 0.0005 | 0.0014 | 0.0015 | 0.0008 | 0.0022 | 0.0002 | 0.0008 | 0.0007 | 0.0019 | 0.0007 | 0.0006 | 0.0008 | 0.0004 |
Cytoplasm | 0.0555 | 0.1035 | 0.0795 | 0.0969 | 0.0721 | 0.0733 | 0.1233 | 0.1943 | 0.1844 | 0.1119 | 0.0733 | 0.1312 | 0.0607 | 0.1297 | 0.1147 | 0.1049 | 0.074 | 0.1195 |
Cytoplasmic Foci | 0.1811 | 0.1852 | 0.1679 | 0.1504 | 0.2363 | 0.1599 | 0.1462 | 0.1268 | 0.132 | 0.1166 | 0.14 | 0.1627 | 0.1509 | 0.1571 | 0.1347 | 0.0826 | 0.1576 | 0.1432 |
Eisosomes | 0.0007 | 0.0003 | 0.0009 | 0.001 | 0.0001 | 0.0003 | 0.002 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0099 | 0.0023 | 0.0047 | 0.0041 | 0.0018 | 0.004 | 0.0134 | 0.0076 | 0.0066 | 0.0088 | 0.0051 | 0.0054 | 0.0072 | 0.0083 | 0.0075 | 0.0049 | 0.0079 | 0.0073 |
Endosome | 0.227 | 0.1939 | 0.1397 | 0.1207 | 0.2156 | 0.1582 | 0.2257 | 0.2065 | 0.2545 | 0.2283 | 0.3814 | 0.2288 | 0.2819 | 0.2681 | 0.2526 | 0.2489 | 0.2485 | 0.2276 |
Golgi | 0.0645 | 0.0679 | 0.0507 | 0.0429 | 0.0567 | 0.0605 | 0.0529 | 0.0704 | 0.0696 | 0.0604 | 0.0796 | 0.0853 | 0.0871 | 0.0815 | 0.0645 | 0.0583 | 0.1012 | 0.0813 |
Lipid Particles | 0.0437 | 0.013 | 0.0189 | 0.0144 | 0.031 | 0.0171 | 0.0385 | 0.0119 | 0.0162 | 0.0146 | 0.0183 | 0.0164 | 0.0466 | 0.0088 | 0.0096 | 0.0034 | 0.0178 | 0.0195 |
Mitochondria | 0.1877 | 0.2021 | 0.3123 | 0.3883 | 0.2498 | 0.2926 | 0.0988 | 0.1431 | 0.1542 | 0.3016 | 0.0645 | 0.226 | 0.1807 | 0.1337 | 0.2255 | 0.3414 | 0.2266 | 0.2764 |
None | 0.1254 | 0.1103 | 0.1105 | 0.0964 | 0.0149 | 0.0967 | 0.1423 | 0.0871 | 0.0705 | 0.021 | 0.1235 | 0.0368 | 0.06 | 0.0903 | 0.0917 | 0.0857 | 0.0185 | 0.0378 |
Nuclear Periphery | 0.0035 | 0.0006 | 0.0017 | 0.0013 | 0.0005 | 0.0009 | 0.0116 | 0.0034 | 0.0035 | 0.0026 | 0.0052 | 0.0057 | 0.0062 | 0.0028 | 0.0026 | 0.0018 | 0.0146 | 0.0041 |
Nucleolus | 0.0042 | 0.0006 | 0.001 | 0.0008 | 0.0004 | 0.0013 | 0.0012 | 0.001 | 0.0036 | 0.0006 | 0.0006 | 0.0009 | 0.0052 | 0.0018 | 0.0008 | 0.0006 | 0.0009 | 0.0014 |
Nucleus | 0.0035 | 0.0009 | 0.0016 | 0.0015 | 0.0009 | 0.005 | 0.0039 | 0.0041 | 0.0023 | 0.0018 | 0.0028 | 0.0038 | 0.005 | 0.0031 | 0.0021 | 0.0013 | 0.006 | 0.0056 |
Peroxisomes | 0.0366 | 0.0618 | 0.0583 | 0.0463 | 0.101 | 0.0886 | 0.0171 | 0.0207 | 0.0192 | 0.0166 | 0.0427 | 0.0251 | 0.0291 | 0.0191 | 0.0312 | 0.0096 | 0.0262 | 0.0325 |
Punctate Nuclear | 0.0036 | 0.001 | 0.0014 | 0.001 | 0.0013 | 0.0014 | 0.0118 | 0.0021 | 0.0026 | 0.0009 | 0.0027 | 0.0045 | 0.0042 | 0.0023 | 0.0013 | 0.0005 | 0.0047 | 0.0031 |
Vacuole | 0.0116 | 0.008 | 0.0078 | 0.0063 | 0.0064 | 0.0075 | 0.0203 | 0.025 | 0.0178 | 0.0251 | 0.0104 | 0.0154 | 0.0174 | 0.0184 | 0.016 | 0.0277 | 0.0253 | 0.0169 |
Vacuole Periphery | 0.004 | 0.0031 | 0.0035 | 0.0033 | 0.0032 | 0.0035 | 0.0061 | 0.0052 | 0.0066 | 0.0183 | 0.008 | 0.0102 | 0.0074 | 0.0045 | 0.0084 | 0.0119 | 0.0147 | 0.008 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.3886 | 5.711 | 3.6324 | 6.1906 | 5.9165 | 8.2094 | 4.3308 | 7.5163 | 5.2313 | 5.2888 |
Translational Efficiency | 0.4325 | 0.6268 | 0.7759 | 0.4774 | 0.4389 | 0.5539 | 1.0301 | 0.7799 | 1.1015 | 0.9041 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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