Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.05
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0 0 0 0 0 0
Golgi 0 0.08 0 0 0.05 0 0 0 0 0 0 0.05 0.07 0.15 0.33 0.31 0.45 0.05 0.08 0 0.09 0.12 0.13
Mitochondria 0.89 0.85 0.92 0.95 0.82 0.9 0.85 0.91 0.9 0.92 0.94 0.89 0.88 0.82 0.1 0.12 0.05 0.79 0.75 0.8 0.76 0.66 0.61
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0.08 0 0.07 0.13 0.07 0.1 0.07 0.09 0.1 0.05 0.05 0 0.05 0.12 0 0 0 0 0 0 0
Nucleolus 0.05 0 0.07 0.06 0.08 0.1 0.08 0.07 0.1 0.09 0.1 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0.06 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.18 0.06 0 0.09 0.07 0.06 0 0 0 0 0.09 0.08 0.08 0.16 0.12 0.21 0.07 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 2 0 0 0 0 0 0 1 3 0 5 0 2 1 0 2 6 0 5 12 17
Bud 4 0 11 7 3 6 11 16 13 7 15 5 6 5 2 0 2 1 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 1 1 0 0 3 6 3 6 5 0 0 0 0 0 0
Cell Periphery 4 3 4 0 2 2 3 0 2 3 3 7 9 9 8 0 2 0 0 0 0 0 0
Cytoplasm 2 3 6 6 14 7 7 7 7 8 7 6 12 16 2 1 3 0 0 0 0 1 3
Endoplasmic Reticulum 1 3 2 2 1 2 2 2 1 1 4 0 2 4 4 1 2 0 0 1 0 0 1
Endosome 3 0 0 0 7 2 5 1 4 1 0 3 5 6 2 2 3 12 2 8 1 10 9
Golgi 6 14 1 1 13 2 1 0 1 1 0 12 28 64 20 5 17 19 14 8 14 35 43
Mitochondria 297 142 189 166 224 310 369 397 488 339 426 217 365 341 6 2 2 283 131 167 126 190 207
Nucleus 3 1 1 0 1 2 4 1 3 3 1 0 0 2 0 1 0 1 0 0 1 0 1
Nuclear Periphery 19 6 16 5 18 45 30 45 39 33 45 13 19 14 3 2 1 2 1 2 0 1 4
Nucleolus 17 5 14 10 21 35 36 30 53 33 46 6 9 11 3 0 2 3 1 1 0 1 2
Peroxisomes 1 3 1 1 5 1 19 4 12 3 7 12 18 8 4 0 1 3 3 8 5 17 15
SpindlePole 0 0 2 0 2 2 6 6 8 2 5 2 3 0 2 0 0 0 0 2 0 1 2
Vac/Vac Membrane 69 31 13 2 25 25 24 17 15 6 7 23 35 33 10 2 8 24 10 2 6 11 20
Unique Cell Count 335 168 205 175 272 344 432 434 540 370 451 244 414 416 61 16 38 359 174 208 166 290 340
Labelled Cell Count 428 211 263 201 336 441 520 532 649 447 574 306 516 513 68 17 43 359 174 208 166 290 340


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.6 12.2 7.1 9.6 11.2 8.9 9.2 8.1 8.4 8.0 8.1 11.2 11.3 11.0 12.7 12.0 12.1 10.7 11.7 11.7
Std Deviation (1e-4) 1.9 2.3 1.7 3.2 3.6 2.4 3.4 2.1 2.5 2.3 3.6 2.3 2.2 2.2 3.3 3.0 3.1 2.9 2.8 2.9
Intensity Change (Log2) 0.43 0.65 0.32 0.38 0.18 0.25 0.17 0.18 0.65 0.67 0.62 0.83 0.75 0.77 0.59 0.71 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.6 -2.7 -2.4 -1.8 -1.0 -2.0 -2.3 -1.2 -1.9 -2.8 -3.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.6 0 0 0 0 0
Cytoplasm 0.3 1.2 0 0 0 0 0 0 -0.3 0 0.6 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 2.7 0 0 0 0 0 0 2.8 3.5 5.7 0 0 0
Mitochondria 1.0 -3.1 -0.8 -2.4 -0.3 -0.8 -0.2 1.1 -1.2 -1.5 -3.4 -12.8 -9.0 -12.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.1 -0.5 1.9 -0.4 1.0 -0.3 0.5 0.9 -1.1 -1.6 -2.4 0 0 0
Nucleolus -0.4 0.4 1.3 0.7 0 1.3 0.9 1.4 -2.2 -2.9 -2.5 0 0 0
Peroxisomes 0 0 0 2.6 0 0 0 0 2.8 2.6 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 1.1 0.4 -0.4 -1.4 -2.3 -3.0 -3.3 1.2 0.9 0.7 2.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6904 9.9354 9.4367 8.0023 9.1051 9.5093 8.8572 8.9412 8.2452 8.523 8.0839 8.5053 9.8311 10.8138 10.0143 9.6511 9.7671 10.0932
Actin 0.0142 0.0032 0.0084 0.0016 0.0012 0.0045 0.0032 0.0009 0.0153 0.0008 0.0021 0.0005 0.0364 0.0037 0.0071 0.0032 0.0009 0.0053
Bud 0.0002 0.0012 0.0003 0.001 0.0001 0 0.0001 0.0001 0.0003 0 0 0.0001 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0031 0.0001 0.0003 0.0003 0 0.0001 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0009 0 0.0001 0.0001 0 0.0003
Bud Periphery 0.0003 0.0016 0.0004 0.0028 0.0003 0.0001 0.0002 0.0003 0.0007 0.0001 0.0001 0.0002 0.001 0.0004 0.0005 0.0006 0.0004 0.0002
Bud Site 0.0007 0.0008 0.0037 0.0011 0.0004 0 0.001 0.0002 0.0013 0.0001 0.0001 0.0002 0.0024 0.0004 0.0018 0.0002 0.0002 0.0004
Cell Periphery 0.0002 0.0002 0.0001 0.0006 0.0001 0.0001 0.0001 0.0007 0.0004 0.0001 0.0008 0.0001 0.0006 0.0001 0.001 0.0001 0.0001 0.0001
Cytoplasm 0.0046 0 0.0027 0.0003 0.0002 0.0002 0.0004 0 0.0003 0.0003 0 0 0.0031 0 0.0046 0 0 0.0002
Cytoplasmic Foci 0.0111 0.0025 0.0103 0.0156 0.005 0.0014 0.0202 0.0034 0.0073 0.0036 0.0009 0.003 0.0225 0.0009 0.0068 0.0012 0.0012 0.0046
Eisosomes 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0009 0.0002 0.0003 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0026 0.0001 0.0013 0.0007 0.0002 0.0005 0.0002 0.0002 0.0002 0.0006 0 0.0001 0.0006 0.0001 0.0014 0.0001 0 0.0004
Endosome 0.013 0.0087 0.0161 0.0301 0.0038 0.0027 0.0114 0.003 0.0064 0.0186 0.0026 0.0035 0.0155 0.0052 0.0159 0.0088 0.0023 0.0064
Golgi 0.0126 0.0209 0.0123 0.0129 0.0046 0.0033 0.0101 0.0055 0.0149 0.0262 0.0222 0.0076 0.0136 0.0198 0.0222 0.0377 0.006 0.0167
Lipid Particles 0.0234 0.0097 0.0124 0.0175 0.0087 0.0069 0.0261 0.0066 0.0107 0.0111 0.0045 0.0073 0.0235 0.0066 0.0111 0.0015 0.0108 0.0066
Mitochondria 0.8766 0.9272 0.9104 0.8183 0.9658 0.9654 0.8844 0.9502 0.9217 0.9313 0.9411 0.969 0.8259 0.9477 0.9039 0.9416 0.9683 0.9419
None 0.0024 0 0.0005 0.0003 0.0007 0.0001 0.0006 0 0.0003 0.0008 0 0.0001 0.0006 0 0.0032 0 0 0.0002
Nuclear Periphery 0.0046 0.0001 0.0025 0.0008 0.0008 0.0019 0.0006 0.0014 0.0003 0.0011 0 0.0002 0.0005 0.0007 0.0073 0 0.0001 0.0016
Nucleolus 0.0004 0.0002 0.0012 0.0007 0.0006 0.0002 0.0012 0.0008 0.0005 0.0003 0.0154 0.0026 0.0035 0.0001 0.0002 0 0.0004 0.0002
Nucleus 0.0075 0.0001 0.0006 0.0006 0.0005 0.0002 0.0007 0.0002 0.0002 0.0002 0.0004 0.0008 0.0009 0 0.0012 0 0.0001 0.0004
Peroxisomes 0.0083 0.0136 0.0083 0.0535 0.0023 0.0055 0.0143 0.0138 0.0149 0.001 0.0061 0.0021 0.0362 0.0093 0.0062 0.0024 0.0027 0.0033
Punctate Nuclear 0.0051 0 0.0013 0.0004 0.0023 0.0004 0.0156 0.0001 0.0007 0.0003 0 0.0002 0.0019 0 0.0013 0 0 0.0006
Vacuole 0.0027 0.0025 0.003 0.0188 0.0007 0.0007 0.0029 0.004 0.0012 0.0011 0.0012 0.0007 0.005 0.0008 0.0017 0.0004 0.0013 0.001
Vacuole Periphery 0.0061 0.0068 0.0039 0.022 0.0014 0.0057 0.006 0.0083 0.0022 0.0022 0.002 0.0015 0.0042 0.0035 0.0021 0.0016 0.0049 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.9871 14.2953 12.3757 21.3108 19.0057 10.655 25.2944 34.957 29.2805 32.0235
Translational Efficiency 1.7319 2.3224 2.888 1.9993 1.9046 6.5055 2.46 1.4357 1.965 1.9368

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2422 1588 321 873 882 2156 2847 1732 3304 3744 3168 2605

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1028.92 1168.73 1378.61 1354.05 896.23 1173.93 1347.92 1356.44 993.50 1171.72 1351.03 1355.64
Standard Deviation 170.22 218.97 247.04 216.21 178.06 228.52 214.18 222.80 182.07 224.53 217.93 220.62
Intensity Change Log 2 0.183811 0.422084 0.396150 0.389405 0.588794 0.597884 0.283177 0.502095 0.493583

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000437 0.003979 0.008081 0.008741 0.001584 0.001458 0.000857 0.005676 0.000743 0.002527 0.001589 0.006703
Bud Neck 0.000182 0.001091 0.006717 0.002128 0.001860 0.001299 0.001173 0.000670 0.000630 0.001211 0.001735 0.001159
Bud Site 0.000926 0.005870 0.002861 0.003854 0.002135 0.001838 0.002576 0.002337 0.001249 0.003548 0.002605 0.002845
Cell Periphery 0.000217 0.000110 0.000184 0.000437 0.000191 0.000103 0.000119 0.000598 0.000210 0.000106 0.000125 0.000544
Cytoplasm 0.000083 0.000715 0.000332 0.000402 0.000172 0.000247 0.000397 0.000328 0.000107 0.000446 0.000390 0.000352
Cytoplasmic Foci 0.000966 0.017169 0.026687 0.011628 0.007015 0.009000 0.006402 0.004267 0.002580 0.012465 0.008457 0.006734
Eisosomes 0.000065 0.000128 0.000597 0.000209 0.000122 0.000098 0.000068 0.000235 0.000080 0.000111 0.000122 0.000226
Endoplasmic Reticulum 0.000033 0.000177 0.000309 0.000539 0.000114 0.000108 0.000065 0.000202 0.000055 0.000137 0.000090 0.000315
Endosome 0.001546 0.009002 0.008603 0.009704 0.007250 0.008398 0.005050 0.003378 0.003069 0.008654 0.005410 0.005498
Golgi 0.003738 0.034963 0.025879 0.041077 0.012876 0.017678 0.011485 0.022574 0.006177 0.025009 0.012943 0.028775
Lipid Particles 0.000807 0.003875 0.006141 0.002401 0.002784 0.002557 0.002294 0.000792 0.001335 0.003116 0.002683 0.001331
Mitochondria 0.985285* 0.894914* 0.852893* 0.877485* 0.946599* 0.938100* 0.945696* 0.942956* 0.974958* 0.919783* 0.936293* 0.921015*
Mitotic Spindle 0.000073 0.003574 0.006332 0.004523 0.000733 0.000758 0.003746 0.001004 0.000249 0.001953 0.004008 0.002183
None 0.000445 0.000947 0.002396 0.000992 0.000538 0.000552 0.000626 0.000492 0.000470 0.000720 0.000806 0.000659
Nuclear Periphery 0.000022 0.000195 0.001673 0.001575 0.000061 0.000105 0.000482 0.000057 0.000032 0.000143 0.000603 0.000566
Nuclear Periphery Foci 0.000018 0.000229 0.001098 0.000791 0.000078 0.000137 0.000285 0.000048 0.000034 0.000176 0.000367 0.000297
Nucleolus 0.000015 0.000157 0.001567 0.000242 0.000063 0.000229 0.000275 0.000063 0.000028 0.000198 0.000406 0.000123
Nucleus 0.000009 0.000085 0.000217 0.000076 0.000039 0.000211 0.000070 0.000019 0.000017 0.000158 0.000085 0.000038
Peroxisomes 0.003034 0.013844 0.022515 0.008339 0.013419 0.008215 0.005237 0.008479 0.005806 0.010602 0.006988 0.008432
Vacuole 0.000798 0.004774 0.011632 0.009506 0.000930 0.004692 0.005767 0.002303 0.000833 0.004727 0.006361 0.004717
Vacuole Periphery 0.001302 0.004203 0.013286 0.015351 0.001437 0.004215 0.007328 0.003524 0.001338 0.004210 0.007932 0.007487

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.60 -4.17 -4.61 -2.50 -0.26 0.23 1.65 -4.20 -4.43 -5.39 -4.93 -3.18 -7.00 -4.61 -5.86
Bud Neck -4.30 -6.39 -4.59 -2.26 4.12 0.75 0.98 1.75 1.87 2.35 -2.14 -4.14 -2.20 0.16 2.24
Bud Site -3.06 -2.61 -2.81 1.08 -0.90 0.32 -0.49 -0.21 -0.64 0.30 -2.85 -2.36 -2.47 0.77 -0.35
Cell Periphery 1.11 0.34 -0.99 -1.63 -1.26 1.92 1.41 -1.70 -2.10 -2.03 1.46 1.14 -1.82 -2.57 -2.44
Cytoplasm -3.34 -4.74 -4.57 1.56 -0.92 -1.67 -3.10 -3.18 -1.46 0.91 -4.01 -4.42 -6.17 1.03 0.55
Cytoplasmic Foci -11.86 -7.22 -7.60 2.84 3.94 -1.51 0.48 2.29 5.42 2.85 -12.67 -8.27 -6.35 6.26 2.02
Eisosomes -6.44 -7.89 -6.52 -3.51 5.49 1.47 3.61 -4.77 -6.88 -8.93 -3.91 -4.31 -9.61 -7.56 -6.43
Endoplasmic Reticulum -4.78 -7.24 -4.50 -3.12 -1.96 0.22 2.32 -1.99 -1.99 -3.20 -4.15 -2.44 -5.54 -3.55 -4.69
Endosome -8.88 -4.28 -7.09 -0.52 -0.56 -0.65 1.35 2.41 5.69 2.90 -7.66 -3.78 -3.80 4.29 -0.14
Golgi -12.40 -5.07 -9.45 -1.33 -2.61 -2.00 0.63 -3.38 -1.93 -4.73 -12.63 -5.55 -11.03 -1.61 -7.27
Lipid Particles -5.05 -3.89 -3.21 2.05 2.62 0.28 0.60 2.57 4.07 3.62 -4.35 -3.19 0.01 4.63 3.37
Mitochondria 15.39 9.31 13.55 1.82 -1.52 1.28 0.17 0.54 -0.93 0.54 14.93 10.85 13.04 -0.24 3.18
Mitotic Spindle -4.06 -3.44 -4.43 -0.72 0.86 -0.07 -4.40 -0.79 -1.06 4.28 -4.41 -6.35 -5.28 -0.44 2.66
None -1.12 -7.49 -8.09 -0.09 5.28 -0.20 -2.05 1.14 0.96 4.13 -1.28 -7.82 -5.47 0.32 3.35
Nuclear Periphery -2.98 -3.80 -2.52 -2.23 0.12 -2.08 -3.81 0.26 2.32 3.85 -4.11 -5.26 -2.58 -2.03 0.15
Nuclear Periphery Foci -2.95 -4.76 -2.79 -1.97 0.85 -2.52 -4.23 1.74 4.53 5.01 -4.38 -6.91 -2.81 -1.23 0.66
Nucleolus -2.95 -1.80 -3.14 -1.01 1.53 -1.96 -2.68 -0.00 1.90 2.59 -3.24 -3.36 -3.16 1.24 2.43
Nucleus -3.73 -2.80 -3.53 0.30 1.83 -1.08 -1.49 2.37 1.21 2.63 -1.52 -3.53 -2.89 1.29 2.33
Peroxisomes -6.48 -3.91 -3.21 2.51 2.74 2.02 3.40 1.86 -0.16 -2.27 -4.01 -1.12 -2.09 1.60 -1.15
Vacuole -4.08 -4.47 -6.31 -2.89 0.74 -5.67 -8.55 -2.87 3.05 4.95 -6.75 -9.59 -6.70 -0.02 2.11
Vacuole Periphery -4.81 -3.44 -8.68 -6.60 -0.54 -5.80 -9.08 -3.74 1.02 4.73 -7.36 -9.66 -9.13 -4.49 0.48
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrp49

Mrp49


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp49-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available