Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0.11 0.06 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.45 0.37 0.5 0.43 0.27 0.24 – 0.16 0.26 0.17 0.26 – – – 0.86 0.93 0.82 0.23 0.23 0.2 0.6 0.45 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.08 0.05 0.05 0 0 0 0 0.06 0
Endosome 0 0 0 0.05 0.05 0 0 – 0 0 0 0 – – – 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.07 0 0 0 0.19 0.14 – 0.43 0.37 0.69 0.37 – – – 0 0 0.05 0.09 0 0.09 0.16 0.07 0
Nucleus 0.27 0.42 0.59 0.37 0.39 0.48 0.56 – 0.5 0.4 0.32 0.37 – – – 0 0 0 0.2 0.29 0.23 0.16 0 0.13
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.11 0
Nucleolus 0 0 0 0 0 0 0 – 0.06 0 0 0 – – – 0 0 0 0.07 0.08 0.06 0 0.12 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 – 0 0.06 0 0.06 – – – 0 0 0 0.13 0.14 0.1 0 0 0.05
Vac/Vac Membrane 0 0.1 0.05 0.05 0.13 0.07 0 – 0 0 0.07 0.06 – – – 0.08 0 0.07 0.11 0.08 0.14 0.06 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 2 0 1 0 0 0
Bud 2 0 0 0 0 0 1 – 3 3 6 5 – – – 0 1 1 10 16 5 0 0 1
Bud Neck 0 0 0 1 1 3 2 – 3 6 2 4 – – – 0 2 0 2 2 3 0 0 0
Bud Site 0 0 0 0 0 0 1 – 3 2 3 6 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 3 3 1 0 0 0 0 0 0
Cytoplasm 97 57 15 56 73 48 63 – 32 50 33 58 – – – 92 124 49 53 35 17 3 3 14
Endoplasmic Reticulum 0 0 0 0 0 0 2 – 1 0 0 0 – – – 9 6 3 1 0 0 0 0 0
Endosome 1 4 1 6 9 4 7 – 1 5 1 2 – – – 4 2 4 9 2 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 2 3 0 0 0 0 0 0 0
Mitochondria 44 9 1 5 2 33 37 – 86 71 133 83 – – – 2 1 3 21 5 8 0 0 0
Nucleus 49 54 24 41 66 86 149 – 100 76 61 82 – – – 1 0 0 46 44 20 0 0 3
Nuclear Periphery 0 0 0 0 0 0 1 – 1 0 1 1 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 8 2 0 2 3 2 5 – 11 5 8 6 – – – 1 0 1 16 11 5 0 0 1
Peroxisomes 0 0 0 1 0 0 0 – 0 1 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 4 6 5 14 – 6 11 1 14 – – – 2 0 1 29 20 8 0 0 1
Vac/Vac Membrane 8 13 2 6 23 12 9 – 3 5 14 14 – – – 9 5 4 25 11 12 0 0 1
Unique Cell Count 182 128 41 112 171 178 266 200 192 193 224 107 133 60 229 152 87 6 8 28
Labelled Cell Count 211 139 43 122 183 193 291 250 235 263 275 125 147 67 229 152 87 6 8 28


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 5.0 5.9 6.2 5.3 5.2 – 4.3 4.4 4.0 4.2 – – – 5.9 6.0 5.5 – – –
Std Deviation (1e-4) 1.0 1.1 1.0 2.7 1.9 2.1 1.8 – 1.6 1.6 1.6 1.3 – – – 1.8 2.2 1.5 – – –
Intensity Change (Log2) – – – 0.23 0.3 0.06 0.06 – -0.23 -0.19 -0.32 -0.28 – – – 0.23 0.24 0.13 – – –

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP70002468WT302468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT102468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3455 -0.2734 -0.402 -0.5283 0.2179 0.242 0.471 -0.5013 -0.4847 -0.2519 -0.8948 -0.1746 0.4378 -1.0231 -1.1856 -1.1207 -1.3538 -0.6267
Actin 0.0292 0.0062 0.0105 0.0231 0.0097 0.0007 0.0064 0.0011 0.0099 0.0047 0.0009 0.0018 0.0231 0.0009 0.0084 0.0015 0.0126 0.0093
Bud 0.0011 0.0121 0.0091 0.0058 0.0053 0.0021 0.0007 0.0352 0.0179 0.0019 0.013 0.009 0.0007 0.0345 0.0121 0.0047 0.0018 0.006
Bud Neck 0.0048 0.0032 0.0102 0.0091 0.0032 0.0089 0.0122 0.004 0.0074 0.004 0.0033 0.0129 0.0037 0.0038 0.0027 0.0031 0.0024 0.0165
Bud Periphery 0.0026 0.0059 0.0105 0.0066 0.036 0.0022 0.0016 0.026 0.0265 0.0034 0.0384 0.0121 0.0013 0.0287 0.012 0.0062 0.0016 0.0074
Bud Site 0.0127 0.0318 0.0108 0.003 0.0018 0.001 0.0062 0.0652 0.014 0.0034 0.006 0.0027 0.0059 0.064 0.0099 0.0018 0.0011 0.0026
Cell Periphery 0.0005 0.0006 0.0003 0.0003 0.0005 0.0001 0.0004 0.0024 0.0009 0.0003 0.0013 0.0005 0.0002 0.0017 0.0006 0.0003 0.0001 0.0003
Cytoplasm 0.0164 0.0963 0.0855 0.0685 0.1131 0.0344 0.0267 0.1488 0.093 0.0948 0.1593 0.036 0.0131 0.139 0.118 0.1142 0.2017 0.0634
Cytoplasmic Foci 0.011 0.0148 0.0136 0.0083 0.0194 0.0029 0.0094 0.0068 0.017 0.0372 0.0237 0.0364 0.029 0.0044 0.0115 0.006 0.0805 0.0057
Eisosomes 0.0003 0.0002 0.0002 0.0005 0.0003 0 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0029 0.0036 0.003 0.0026 0.0067 0.0008 0.0068 0.0039 0.003 0.0026 0.0048 0.0008 0.003 0.0053 0.0058 0.0029 0.0052 0.0012
Endosome 0.0224 0.0064 0.0066 0.0088 0.0373 0.002 0.0096 0.0029 0.0212 0.0355 0.0048 0.0085 0.0219 0.0024 0.0072 0.0016 0.0177 0.0029
Golgi 0.0085 0.0011 0.003 0.0046 0.0183 0.0001 0.0087 0.0003 0.0144 0.0133 0.0013 0.0072 0.007 0.0002 0.0028 0.0001 0.0069 0.0026
Lipid Particles 0.0129 0.0016 0.0017 0.0234 0.0373 0.0005 0.0146 0.0014 0.0194 0.0103 0.0045 0.0102 0.016 0.0004 0.0039 0.0003 0.0246 0.0034
Mitochondria 0.013 0.003 0.0028 0.0056 0.0209 0.0011 0.0328 0.0037 0.0266 0.0563 0.0019 0.0025 0.0033 0.003 0.0057 0.0022 0.0024 0.0018
None 0.033 0.3242 0.2336 0.4066 0.301 0.0656 0.0319 0.2196 0.3476 0.4959 0.5909 0.1017 0.0155 0.2456 0.3053 0.5039 0.3677 0.0772
Nuclear Periphery 0.0154 0.0128 0.0145 0.0104 0.0246 0.0073 0.0047 0.0094 0.0089 0.0058 0.004 0.0062 0.0106 0.0107 0.0201 0.0074 0.0101 0.009
Nucleolus 0.0167 0.0205 0.0206 0.013 0.0155 0.0467 0.0134 0.0287 0.0171 0.0058 0.0062 0.0342 0.0129 0.0272 0.0161 0.0076 0.0072 0.0477
Nucleus 0.7585 0.3422 0.4472 0.3262 0.232 0.7432 0.8002 0.3711 0.3108 0.1682 0.0905 0.6483 0.7616 0.369 0.3977 0.3055 0.0847 0.6793
Peroxisomes 0.0077 0.0072 0.0031 0.02 0.029 0.0002 0.0075 0.0006 0.0073 0.0173 0.0018 0.0046 0.0307 0.0004 0.0055 0.0005 0.0352 0.0042
Punctate Nuclear 0.0256 0.101 0.1086 0.0493 0.0701 0.0783 0.0031 0.0578 0.0278 0.0336 0.0305 0.0611 0.0365 0.0471 0.0463 0.0271 0.1308 0.0562
Vacuole 0.0028 0.0044 0.0037 0.0034 0.0113 0.0016 0.0024 0.0098 0.0067 0.0043 0.011 0.0027 0.0026 0.01 0.0065 0.0024 0.0047 0.0027
Vacuole Periphery 0.0016 0.0007 0.0009 0.001 0.0068 0.0004 0.0007 0.0013 0.0022 0.0013 0.0016 0.0005 0.0012 0.0016 0.0017 0.0007 0.0008 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.869 7.2569 65.0332 167.126 29.5514 8.1134 5.362 18.8567 78.2472 49.6656
Translational Efficiency 0.5744 0.5262 1.3569 0.7754 1.008 0.9677 0.9036 0.7872 1.6648 1.1576

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1813 1114 852 1330 1809 1974 348 24 3622 3088 1200 1354

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 598.78 724.85 977.82 893.80 701.77 717.72 986.53 1010.01 650.22 720.29 980.35 895.86
Standard Deviation 79.86 97.62 124.73 151.18 82.17 96.46 131.47 146.35 96.00 96.94 126.78 151.87
Intensity Change Log 2 — 0.275656 0.707543 0.577926 — 0.032423 0.491365 0.525299 — 0.149519 0.594930 0.549768

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000116 0.000617 0.001379 0.001271 0.000102 0.000403 0.001418 0.001452 0.000109 0.000480 0.001391 0.001274
Bud Neck 0.026656 0.028984 0.015516 0.030698 0.013374 0.028573 0.013892 0.003181 0.020022 0.028721 0.015045 0.030210
Bud Site 0.008960 0.010275 0.008237 0.021226 0.002303 0.012871 0.004365 0.004766 0.005635 0.011935 0.007114 0.020934
Cell Periphery 0.000699 0.000319 0.000260 0.000289 0.000306 0.000496 0.000242 0.000164 0.000503 0.000432 0.000255 0.000287
Cytoplasm 0.357239 0.216393 0.134834 0.093101 0.264457 0.204206 0.066518 0.073543 0.310899 0.208603 0.115023 0.092755
Cytoplasmic Foci 0.090338 0.090699 0.004927 0.004979 0.057951 0.062942 0.002429 0.001475 0.074163 0.072956 0.004202 0.004917
Eisosomes 0.000149 0.000094 0.000053 0.000041 0.000059 0.000085 0.000072 0.000054 0.000104 0.000088 0.000059 0.000041
Endoplasmic Reticulum 0.001072 0.002973 0.008265 0.006768 0.001031 0.002402 0.009359 0.015378 0.001051 0.002608 0.008582 0.006921
Endosome 0.004250 0.010975 0.003178 0.004085 0.004298 0.009666 0.002050 0.001535 0.004274 0.010138 0.002851 0.004039
Golgi 0.001209 0.002067 0.000314 0.005290 0.000357 0.002374 0.000189 0.000146 0.000783 0.002264 0.000277 0.005199
Lipid Particles 0.007242 0.007413 0.001519 0.000496 0.004389 0.008369 0.001680 0.000969 0.005817 0.008024 0.001566 0.000504
Mitochondria 0.003987 0.016485 0.002131 0.007619 0.002903 0.016443 0.001591 0.000869 0.003445 0.016458 0.001974 0.007499
Mitotic Spindle 0.000573 0.008679 0.016896 0.027525 0.000605 0.011178 0.004756 0.063959 0.000589 0.010277 0.013375 0.028171
None 0.043876 0.016560 0.008265 0.008247 0.021302 0.025181 0.015757 0.001123 0.032601 0.022071 0.010437 0.008120
Nuclear Periphery 0.000734 0.001422 0.001872 0.002220 0.001165 0.001634 0.002077 0.001211 0.000949 0.001557 0.001932 0.002202
Nuclear Periphery Foci 0.000764 0.001041 0.002649 0.001099 0.000445 0.001038 0.001218 0.001045 0.000605 0.001039 0.002234 0.001098
Nucleolus 0.024275 0.033143 0.008390 0.006348 0.022520 0.044260 0.008824 0.001451 0.023399 0.040249 0.008516 0.006261
Nucleus 0.410010 0.515539 0.744277 0.754841 0.590461 0.541325 0.836374 0.806534 0.500136 0.532023 0.770985 0.755757
Peroxisomes 0.003144 0.010842 0.000685 0.000535 0.002066 0.008279 0.000387 0.000294 0.002605 0.009204 0.000598 0.000531
Vacuole 0.013604 0.023402 0.035773 0.021127 0.009288 0.016289 0.026512 0.020677 0.011448 0.018855 0.033087 0.021119
Vacuole Periphery 0.001103 0.002079 0.000581 0.002194 0.000618 0.001985 0.000291 0.000176 0.000861 0.002019 0.000497 0.002158

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.08 -12.27 -14.46 -6.13 2.32 -11.60 -13.48 -3.62 -3.19 -1.09 -13.14 -16.74 -15.01 -9.57 3.22
Bud Neck -0.87 4.69 -3.30 -2.01 -7.84 -8.47 -3.39 2.06 7.37 4.19 -5.56 1.31 -6.85 -2.53 -7.49
Bud Site -0.76 0.23 -3.11 -2.38 -3.28 -7.93 -7.46 -1.96 2.74 0.14 -5.74 -2.62 -5.13 -1.70 -3.74
Cell Periphery 4.41 5.31 5.32 2.35 0.03 -4.09 2.30 1.54 4.31 0.32 1.44 5.70 5.55 6.07 -0.13
Cytoplasm 13.72 26.84 35.13 15.29 4.41 6.94 24.31 5.69 3.96 -0.48 15.46 32.02 36.34 20.36 2.32
Cytoplasmic Foci -0.40 24.83 25.35 18.15 1.84 -1.09 18.88 18.53 20.25 0.28 0.17 30.59 30.84 26.97 1.13
Eisosomes 3.07 4.98 5.89 10.23 3.20 -7.70 -5.24 -1.59 -0.39 -0.04 1.56 3.35 5.97 12.04 5.48
Endoplasmic Reticulum -8.15 -17.77 -17.55 -12.67 2.34 -8.15 -12.47 -2.78 -2.60 -1.27 -10.96 -21.87 -17.99 -14.22 3.08
Endosome -6.98 2.20 3.86 8.63 2.28 -6.42 4.24 3.73 8.74 0.90 -9.47 3.87 4.31 11.52 1.77
Golgi -2.31 3.91 -1.01 -0.00 -2.11 -5.47 2.66 2.66 5.91 1.08 -5.27 4.15 -1.53 0.29 -2.14
Lipid Particles -0.51 8.86 10.81 10.99 6.97 -5.35 7.68 6.66 9.09 0.85 -3.91 11.60 15.20 14.92 7.96
Mitochondria -5.85 5.29 -0.71 5.14 -2.92 -8.36 3.06 4.52 9.55 2.82 -10.10 5.56 -1.27 7.69 -2.99
Mitotic Spindle -5.71 -6.11 -8.19 -4.88 -2.12 -7.39 -5.18 -1.56 -1.30 -1.34 -9.19 -7.31 -8.31 -4.61 -3.07
None 9.44 13.92 13.99 7.35 -0.04 -1.79 4.88 15.68 16.76 3.11 6.00 15.29 16.96 13.78 0.62
Nuclear Periphery -5.46 -13.25 -12.76 -8.56 -4.89 -3.68 -8.52 -2.13 -0.92 1.81 -6.74 -14.92 -12.02 -8.59 -4.37
Nuclear Periphery Foci -1.21 -3.94 -1.39 -0.34 3.56 -2.89 -4.36 -1.84 -0.03 0.39 -2.64 -4.62 -2.89 0.12 3.63
Nucleolus -3.15 7.37 10.97 12.42 2.75 -8.48 5.31 13.87 20.02 4.28 -8.82 9.46 15.18 21.59 3.49
Nucleus -8.08 -27.97 -33.85 -19.65 -1.41 4.60 -17.06 -2.53 -3.27 0.82 -3.59 -28.52 -28.32 -23.99 0.48
Peroxisomes -7.16 6.77 7.78 10.20 2.27 -8.40 7.37 6.48 11.13 0.06 -10.74 9.29 10.69 15.31 2.44
Vacuole -5.53 -9.60 -10.06 -6.24 1.97 -5.46 -6.19 -2.16 -1.80 -0.07 -7.09 -11.70 -11.14 -8.46 1.79
Vacuole Periphery -3.43 2.57 0.35 3.33 -1.66 -9.76 8.09 6.47 12.12 1.45 -7.47 3.41 -0.36 3.59 -1.87
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
Localization
Cell Percentages nucleus (59%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ash1

Ash1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ash1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
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