Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0.11 0.06 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.45 0.37 0.5 0.43 0.27 0.24 – 0.16 0.26 0.17 0.26 – – – 0.86 0.93 0.82 0.23 0.23 0.2 0.6 0.45 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.08 0.05 0.05 0 0 0 0 0.06 0
Endosome 0 0 0 0.05 0.05 0 0 – 0 0 0 0 – – – 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.07 0 0 0 0.19 0.14 – 0.43 0.37 0.69 0.37 – – – 0 0 0.05 0.09 0 0.09 0.16 0.07 0
Nucleus 0.27 0.42 0.59 0.37 0.39 0.48 0.56 – 0.5 0.4 0.32 0.37 – – – 0 0 0 0.2 0.29 0.23 0.16 0 0.13
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.11 0
Nucleolus 0 0 0 0 0 0 0 – 0.06 0 0 0 – – – 0 0 0 0.07 0.08 0.06 0 0.12 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 – 0 0.06 0 0.06 – – – 0 0 0 0.13 0.14 0.1 0 0 0.05
Vac/Vac Membrane 0 0.1 0.05 0.05 0.13 0.07 0 – 0 0 0.07 0.06 – – – 0.08 0 0.07 0.11 0.08 0.14 0.06 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 2 0 1 0 0 0
Bud 2 0 0 0 0 0 1 – 3 3 6 5 – – – 0 1 1 10 16 5 0 0 1
Bud Neck 0 0 0 1 1 3 2 – 3 6 2 4 – – – 0 2 0 2 2 3 0 0 0
Bud Site 0 0 0 0 0 0 1 – 3 2 3 6 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 3 3 1 0 0 0 0 0 0
Cytoplasm 97 57 15 56 73 48 63 – 32 50 33 58 – – – 92 124 49 53 35 17 3 3 14
Endoplasmic Reticulum 0 0 0 0 0 0 2 – 1 0 0 0 – – – 9 6 3 1 0 0 0 0 0
Endosome 1 4 1 6 9 4 7 – 1 5 1 2 – – – 4 2 4 9 2 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 2 3 0 0 0 0 0 0 0
Mitochondria 44 9 1 5 2 33 37 – 86 71 133 83 – – – 2 1 3 21 5 8 0 0 0
Nucleus 49 54 24 41 66 86 149 – 100 76 61 82 – – – 1 0 0 46 44 20 0 0 3
Nuclear Periphery 0 0 0 0 0 0 1 – 1 0 1 1 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 8 2 0 2 3 2 5 – 11 5 8 6 – – – 1 0 1 16 11 5 0 0 1
Peroxisomes 0 0 0 1 0 0 0 – 0 1 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 4 6 5 14 – 6 11 1 14 – – – 2 0 1 29 20 8 0 0 1
Vac/Vac Membrane 8 13 2 6 23 12 9 – 3 5 14 14 – – – 9 5 4 25 11 12 0 0 1
Unique Cell Count 182 128 41 112 171 178 266 200 192 193 224 107 133 60 229 152 87 6 8 28
Labelled Cell Count 211 139 43 122 183 193 291 250 235 263 275 125 147 67 229 152 87 6 8 28


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 5.0 5.9 6.2 5.3 5.2 – 4.3 4.4 4.0 4.2 – – – 5.9 6.0 5.5 – – –
Std Deviation (1e-4) 1.0 1.1 1.0 2.7 1.9 2.1 1.8 – 1.6 1.6 1.6 1.3 – – – 1.8 2.2 1.5 – – –
Intensity Change (Log2) – – – 0.23 0.3 0.06 0.06 – -0.23 -0.19 -0.32 -0.28 – – – 0.23 0.24 0.13 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3455 -0.2734 -0.402 -0.5283 0.2179 0.242 0.471 -0.5013 -0.4847 -0.2519 -0.8948 -0.1746 0.4378 -1.0231 -1.1856 -1.1207 -1.3538 -0.6267
Actin 0.0292 0.0062 0.0105 0.0231 0.0097 0.0007 0.0064 0.0011 0.0099 0.0047 0.0009 0.0018 0.0231 0.0009 0.0084 0.0015 0.0126 0.0093
Bud 0.0011 0.0121 0.0091 0.0058 0.0053 0.0021 0.0007 0.0352 0.0179 0.0019 0.013 0.009 0.0007 0.0345 0.0121 0.0047 0.0018 0.006
Bud Neck 0.0048 0.0032 0.0102 0.0091 0.0032 0.0089 0.0122 0.004 0.0074 0.004 0.0033 0.0129 0.0037 0.0038 0.0027 0.0031 0.0024 0.0165
Bud Periphery 0.0026 0.0059 0.0105 0.0066 0.036 0.0022 0.0016 0.026 0.0265 0.0034 0.0384 0.0121 0.0013 0.0287 0.012 0.0062 0.0016 0.0074
Bud Site 0.0127 0.0318 0.0108 0.003 0.0018 0.001 0.0062 0.0652 0.014 0.0034 0.006 0.0027 0.0059 0.064 0.0099 0.0018 0.0011 0.0026
Cell Periphery 0.0005 0.0006 0.0003 0.0003 0.0005 0.0001 0.0004 0.0024 0.0009 0.0003 0.0013 0.0005 0.0002 0.0017 0.0006 0.0003 0.0001 0.0003
Cytoplasm 0.0164 0.0963 0.0855 0.0685 0.1131 0.0344 0.0267 0.1488 0.093 0.0948 0.1593 0.036 0.0131 0.139 0.118 0.1142 0.2017 0.0634
Cytoplasmic Foci 0.011 0.0148 0.0136 0.0083 0.0194 0.0029 0.0094 0.0068 0.017 0.0372 0.0237 0.0364 0.029 0.0044 0.0115 0.006 0.0805 0.0057
Eisosomes 0.0003 0.0002 0.0002 0.0005 0.0003 0 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0029 0.0036 0.003 0.0026 0.0067 0.0008 0.0068 0.0039 0.003 0.0026 0.0048 0.0008 0.003 0.0053 0.0058 0.0029 0.0052 0.0012
Endosome 0.0224 0.0064 0.0066 0.0088 0.0373 0.002 0.0096 0.0029 0.0212 0.0355 0.0048 0.0085 0.0219 0.0024 0.0072 0.0016 0.0177 0.0029
Golgi 0.0085 0.0011 0.003 0.0046 0.0183 0.0001 0.0087 0.0003 0.0144 0.0133 0.0013 0.0072 0.007 0.0002 0.0028 0.0001 0.0069 0.0026
Lipid Particles 0.0129 0.0016 0.0017 0.0234 0.0373 0.0005 0.0146 0.0014 0.0194 0.0103 0.0045 0.0102 0.016 0.0004 0.0039 0.0003 0.0246 0.0034
Mitochondria 0.013 0.003 0.0028 0.0056 0.0209 0.0011 0.0328 0.0037 0.0266 0.0563 0.0019 0.0025 0.0033 0.003 0.0057 0.0022 0.0024 0.0018
None 0.033 0.3242 0.2336 0.4066 0.301 0.0656 0.0319 0.2196 0.3476 0.4959 0.5909 0.1017 0.0155 0.2456 0.3053 0.5039 0.3677 0.0772
Nuclear Periphery 0.0154 0.0128 0.0145 0.0104 0.0246 0.0073 0.0047 0.0094 0.0089 0.0058 0.004 0.0062 0.0106 0.0107 0.0201 0.0074 0.0101 0.009
Nucleolus 0.0167 0.0205 0.0206 0.013 0.0155 0.0467 0.0134 0.0287 0.0171 0.0058 0.0062 0.0342 0.0129 0.0272 0.0161 0.0076 0.0072 0.0477
Nucleus 0.7585 0.3422 0.4472 0.3262 0.232 0.7432 0.8002 0.3711 0.3108 0.1682 0.0905 0.6483 0.7616 0.369 0.3977 0.3055 0.0847 0.6793
Peroxisomes 0.0077 0.0072 0.0031 0.02 0.029 0.0002 0.0075 0.0006 0.0073 0.0173 0.0018 0.0046 0.0307 0.0004 0.0055 0.0005 0.0352 0.0042
Punctate Nuclear 0.0256 0.101 0.1086 0.0493 0.0701 0.0783 0.0031 0.0578 0.0278 0.0336 0.0305 0.0611 0.0365 0.0471 0.0463 0.0271 0.1308 0.0562
Vacuole 0.0028 0.0044 0.0037 0.0034 0.0113 0.0016 0.0024 0.0098 0.0067 0.0043 0.011 0.0027 0.0026 0.01 0.0065 0.0024 0.0047 0.0027
Vacuole Periphery 0.0016 0.0007 0.0009 0.001 0.0068 0.0004 0.0007 0.0013 0.0022 0.0013 0.0016 0.0005 0.0012 0.0016 0.0017 0.0007 0.0008 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.869 7.2569 65.0332 167.126 29.5514 8.1134 5.362 18.8567 78.2472 49.6656
Translational Efficiency 0.5744 0.5262 1.3569 0.7754 1.008 0.9677 0.9036 0.7872 1.6648 1.1576

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1813 1114 852 1330 1809 1974 348 24 3622 3088 1200 1354

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 598.78 724.85 977.82 893.80 701.77 717.72 986.53 1010.01 650.22 720.29 980.35 895.86
Standard Deviation 79.86 97.62 124.73 151.18 82.17 96.46 131.47 146.35 96.00 96.94 126.78 151.87
Intensity Change Log 2 — 0.275656 0.707543 0.577926 — 0.032423 0.491365 0.525299 — 0.149519 0.594930 0.549768

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000116 0.000617 0.001379 0.001271 0.000102 0.000403 0.001418 0.001452 0.000109 0.000480 0.001391 0.001274
Bud Neck 0.026656 0.028984 0.015516 0.030698 0.013374 0.028573 0.013892 0.003181 0.020022 0.028721 0.015045 0.030210
Bud Site 0.008960 0.010275 0.008237 0.021226 0.002303 0.012871 0.004365 0.004766 0.005635 0.011935 0.007114 0.020934
Cell Periphery 0.000699 0.000319 0.000260 0.000289 0.000306 0.000496 0.000242 0.000164 0.000503 0.000432 0.000255 0.000287
Cytoplasm 0.357239* 0.216393* 0.134834* 0.093101 0.264457* 0.204206* 0.066518 0.073543 0.310899* 0.208603* 0.115023* 0.092755
Cytoplasmic Foci 0.090338 0.090699 0.004927 0.004979 0.057951 0.062942 0.002429 0.001475 0.074163 0.072956 0.004202 0.004917
Eisosomes 0.000149 0.000094 0.000053 0.000041 0.000059 0.000085 0.000072 0.000054 0.000104 0.000088 0.000059 0.000041
Endoplasmic Reticulum 0.001072 0.002973 0.008265 0.006768 0.001031 0.002402 0.009359 0.015378 0.001051 0.002608 0.008582 0.006921
Endosome 0.004250 0.010975 0.003178 0.004085 0.004298 0.009666 0.002050 0.001535 0.004274 0.010138 0.002851 0.004039
Golgi 0.001209 0.002067 0.000314 0.005290 0.000357 0.002374 0.000189 0.000146 0.000783 0.002264 0.000277 0.005199
Lipid Particles 0.007242 0.007413 0.001519 0.000496 0.004389 0.008369 0.001680 0.000969 0.005817 0.008024 0.001566 0.000504
Mitochondria 0.003987 0.016485 0.002131 0.007619 0.002903 0.016443 0.001591 0.000869 0.003445 0.016458 0.001974 0.007499
Mitotic Spindle 0.000573 0.008679 0.016896 0.027525 0.000605 0.011178 0.004756 0.063959 0.000589 0.010277 0.013375 0.028171
None 0.043876 0.016560 0.008265 0.008247 0.021302 0.025181 0.015757 0.001123 0.032601 0.022071 0.010437 0.008120
Nuclear Periphery 0.000734 0.001422 0.001872 0.002220 0.001165 0.001634 0.002077 0.001211 0.000949 0.001557 0.001932 0.002202
Nuclear Periphery Foci 0.000764 0.001041 0.002649 0.001099 0.000445 0.001038 0.001218 0.001045 0.000605 0.001039 0.002234 0.001098
Nucleolus 0.024275 0.033143 0.008390 0.006348 0.022520 0.044260 0.008824 0.001451 0.023399 0.040249 0.008516 0.006261
Nucleus 0.410010* 0.515539* 0.744277* 0.754841* 0.590461* 0.541325* 0.836374* 0.806534* 0.500136* 0.532023* 0.770985* 0.755757*
Peroxisomes 0.003144 0.010842 0.000685 0.000535 0.002066 0.008279 0.000387 0.000294 0.002605 0.009204 0.000598 0.000531
Vacuole 0.013604 0.023402 0.035773 0.021127 0.009288 0.016289 0.026512 0.020677 0.011448 0.018855 0.033087 0.021119
Vacuole Periphery 0.001103 0.002079 0.000581 0.002194 0.000618 0.001985 0.000291 0.000176 0.000861 0.002019 0.000497 0.002158

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.08 -12.27 -14.46 -6.13 2.32 -11.60 -13.48 -3.62 -3.19 -1.09 -13.14 -16.74 -15.01 -9.57 3.22
Bud Neck -0.87 4.69 -3.30 -2.01 -7.84 -8.47 -3.39 2.06 7.37 4.19 -5.56 1.31 -6.85 -2.53 -7.49
Bud Site -0.76 0.23 -3.11 -2.38 -3.28 -7.93 -7.46 -1.96 2.74 0.14 -5.74 -2.62 -5.13 -1.70 -3.74
Cell Periphery 4.41 5.31 5.32 2.35 0.03 -4.09 2.30 1.54 4.31 0.32 1.44 5.70 5.55 6.07 -0.13
Cytoplasm 13.72 26.84 35.13 15.29 4.41 6.94 24.31 5.69 3.96 -0.48 15.46 32.02 36.34 20.36 2.32
Cytoplasmic Foci -0.40 24.83 25.35 18.15 1.84 -1.09 18.88 18.53 20.25 0.28 0.17 30.59 30.84 26.97 1.13
Eisosomes 3.07 4.98 5.89 10.23 3.20 -7.70 -5.24 -1.59 -0.39 -0.04 1.56 3.35 5.97 12.04 5.48
Endoplasmic Reticulum -8.15 -17.77 -17.55 -12.67 2.34 -8.15 -12.47 -2.78 -2.60 -1.27 -10.96 -21.87 -17.99 -14.22 3.08
Endosome -6.98 2.20 3.86 8.63 2.28 -6.42 4.24 3.73 8.74 0.90 -9.47 3.87 4.31 11.52 1.77
Golgi -2.31 3.91 -1.01 -0.00 -2.11 -5.47 2.66 2.66 5.91 1.08 -5.27 4.15 -1.53 0.29 -2.14
Lipid Particles -0.51 8.86 10.81 10.99 6.97 -5.35 7.68 6.66 9.09 0.85 -3.91 11.60 15.20 14.92 7.96
Mitochondria -5.85 5.29 -0.71 5.14 -2.92 -8.36 3.06 4.52 9.55 2.82 -10.10 5.56 -1.27 7.69 -2.99
Mitotic Spindle -5.71 -6.11 -8.19 -4.88 -2.12 -7.39 -5.18 -1.56 -1.30 -1.34 -9.19 -7.31 -8.31 -4.61 -3.07
None 9.44 13.92 13.99 7.35 -0.04 -1.79 4.88 15.68 16.76 3.11 6.00 15.29 16.96 13.78 0.62
Nuclear Periphery -5.46 -13.25 -12.76 -8.56 -4.89 -3.68 -8.52 -2.13 -0.92 1.81 -6.74 -14.92 -12.02 -8.59 -4.37
Nuclear Periphery Foci -1.21 -3.94 -1.39 -0.34 3.56 -2.89 -4.36 -1.84 -0.03 0.39 -2.64 -4.62 -2.89 0.12 3.63
Nucleolus -3.15 7.37 10.97 12.42 2.75 -8.48 5.31 13.87 20.02 4.28 -8.82 9.46 15.18 21.59 3.49
Nucleus -8.08 -27.97 -33.85 -19.65 -1.41 4.60 -17.06 -2.53 -3.27 0.82 -3.59 -28.52 -28.32 -23.99 0.48
Peroxisomes -7.16 6.77 7.78 10.20 2.27 -8.40 7.37 6.48 11.13 0.06 -10.74 9.29 10.69 15.31 2.44
Vacuole -5.53 -9.60 -10.06 -6.24 1.97 -5.46 -6.19 -2.16 -1.80 -0.07 -7.09 -11.70 -11.14 -8.46 1.79
Vacuole Periphery -3.43 2.57 0.35 3.33 -1.66 -9.76 8.09 6.47 12.12 1.45 -7.47 3.41 -0.36 3.59 -1.87
Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
Localization
Cell Percentages nucleus (59%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ash1

Ash1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ash1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available