Standard name
Human Ortholog
Description Protein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.99 0.99 0.98 0.96 0.93 0.92 0.87 0.85 0.75 0.86 0.99 0.99 0.99 0.95 0.89 0.95 0.73 0.83 0.7 0.69 0.69 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.05 0 0 0 0.06 0.07 0.1 0.24 0.28 0.33 0.21 0 0 0 0 0 0 0.12 0.07 0.17 0.18 0.19 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 1 3 3
Bud 0 2 0 0 1 0 4 1 2 7 8 2 1 0 2 0 0 0 0 2 0 1 2 5
Bud Neck 0 3 6 5 12 7 21 13 10 9 5 15 0 0 0 2 2 0 1 0 0 0 3 7
Bud Site 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 7 7 2 1 4 0 2 1 1 2 1 2 0 3 2 1 1 2 6 1 7 4
Cytoplasm 134 218 217 268 294 310 400 345 311 352 208 291 356 371 385 158 143 106 111 177 136 94 230 271
Endoplasmic Reticulum 3 3 0 0 0 0 1 0 0 0 0 0 1 1 0 19 8 6 1 0 1 0 2 5
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 3 1 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 2 0 0 0 0 0
Mitochondria 13 12 0 1 3 18 28 37 84 117 92 71 3 3 0 3 5 3 18 14 33 24 63 42
Nucleus 0 0 1 1 0 1 1 1 2 0 3 2 0 1 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 2 6 6 11 4 7 6 11 0 0 1 1 2 1 1 1 1 0 3 2
Vac/Vac Membrane 0 0 0 0 1 1 0 0 1 2 1 1 1 0 1 1 4 3 3 4 1 7 10 23
Unique Cell Count 143 226 220 271 300 322 429 375 356 415 277 340 359 373 387 166 160 112 152 214 195 138 335 372
Labelled Cell Count 150 239 232 283 315 344 465 409 416 496 328 395 364 378 390 194 169 124 152 214 195 138 335 372


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 2.9 4.3 4.1 4.5 3.4 3.7 3.8 3.0 3.0 2.9 3.3 3.7 3.7 3.7 5.1 5.5 5.4 3.4 3.4 3.9
Std Deviation (1e-4) 0.7 0.5 1.1 1.7 1.7 1.5 1.6 1.3 1.1 1.1 1.4 1.0 1.1 0.9 1.0 1.2 1.4 1.6 1.0 1.0 1.6
Intensity Change (Log2) – – – -0.07 0.06 -0.33 -0.22 -0.17 -0.52 -0.53 -0.55 -0.37 -0.22 -0.2 -0.22 0.25 0.37 0.33 -0.35 -0.32 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8117 -1.5839 -1.3033 -1.7877 -1.0889 -1.5057 -0.7762 -0.959 -1.0055 -1.1806 -0.7373 -0.9556 -2.0994 -1.8156 -1.782 -2.1542 -1.5063 -1.8582
Actin 0.0871 0.0019 0.0366 0.0507 0.0919 0.0087 0.0257 0.0015 0.001 0.0007 0.0087 0.0014 0.0133 0 0.0288 0 0.0652 0.0007
Bud 0.0023 0.0044 0.0006 0.0002 0.0006 0.0005 0.0007 0.0016 0.0007 0.0001 0.0002 0.0003 0.0001 0 0.0033 0 0.0017 0.0007
Bud Neck 0.0065 0.0002 0.0094 0.0009 0.0042 0.0034 0.0025 0.0002 0.0002 0.0001 0.0001 0.0007 0.0004 0 0.0011 0 0.0004 0.0249
Bud Periphery 0.0041 0.0041 0.0013 0.0005 0.0009 0.0024 0.001 0.0017 0.0012 0.0001 0.0004 0.0007 0.0002 0 0.0061 0 0.0044 0.0025
Bud Site 0.0058 0.0111 0.0045 0.0008 0.0029 0.0011 0.0142 0.0048 0.0007 0.0002 0.0003 0.0002 0.003 0 0.0228 0 0.0012 0.0005
Cell Periphery 0.0004 0.0003 0.0003 0.0001 0.0004 0.0003 0.0003 0.0005 0.0047 0 0.0001 0.0001 0.0001 0 0.0007 0 0.0002 0.0007
Cytoplasm 0.015 0.0159 0.0094 0.01 0.0164 0.0359 0.031 0.0483 0.047 0.0612 0.0366 0.0963 0.0141 0.0578 0.0333 0.0143 0.012 0.0825
Cytoplasmic Foci 0.0448 0.0117 0.0293 0.0063 0.0074 0.0137 0.0152 0.008 0.0099 0.0369 0.0078 0.0132 0.0034 0.0139 0.0138 0.0008 0.0025 0.0012
Eisosomes 0.0031 0.0012 0.0005 0.001 0.002 0.0007 0.0009 0.0002 0.0002 0.0001 0.0003 0.0001 0.0005 0.0001 0.0003 0 0.0004 0.0001
Endoplasmic Reticulum 0.0041 0.0006 0.0028 0.0005 0.0062 0.0015 0.0039 0.0009 0.0013 0.0003 0.0037 0.0016 0.0005 0.0002 0.0004 0.0001 0.0015 0.0004
Endosome 0.0235 0.0002 0.0093 0.0045 0.019 0.0044 0.0203 0.0036 0.0188 0.0136 0.0435 0.0081 0.0009 0.0047 0.0032 0 0.0095 0.0001
Golgi 0.007 0.0001 0.0044 0.0065 0.0061 0.0014 0.0051 0.001 0.0014 0.0094 0.0201 0.0038 0.0006 0.0012 0.0008 0 0.013 0
Lipid Particles 0.0152 0.0014 0.0112 0.0235 0.0154 0.0032 0.0136 0.0013 0.0009 0.007 0.0322 0.0064 0.0007 0.0019 0.0016 0 0.0024 0
Mitochondria 0.0083 0.0013 0.0052 0.0095 0.0535 0.0005 0.0361 0.0011 0.0015 0.0021 0.0204 0.0124 0.0002 0 0.0005 0 0.0051 0.0001
None 0.7029 0.9424 0.8166 0.8539 0.6838 0.907 0.7841 0.9148 0.8945 0.8504 0.8159 0.8467 0.9603 0.9165 0.8751 0.9841 0.8763 0.8841
Nuclear Periphery 0.0189 0.0003 0.0136 0.0008 0.0271 0.0034 0.0144 0.0003 0.0019 0.0002 0.0026 0.0007 0.0003 0.0001 0.0005 0.0001 0.0013 0.0001
Nucleolus 0.0021 0.0004 0.0005 0.0003 0.0026 0.0011 0.0011 0.0003 0.0002 0.0002 0.0005 0.0001 0.0002 0 0.0003 0 0.0001 0.0001
Nucleus 0.0066 0.0007 0.0027 0.0004 0.0318 0.0022 0.0052 0.0007 0.0013 0.0004 0.0005 0.0004 0.0002 0.0001 0.0004 0.0001 0.0002 0.0008
Peroxisomes 0.0232 0.0006 0.0266 0.0268 0.0129 0.0006 0.0155 0.0073 0.0014 0.0137 0.0017 0.003 0.0003 0.0031 0.0021 0 0.0011 0
Punctate Nuclear 0.0166 0.001 0.0126 0.0021 0.0099 0.007 0.0056 0.0006 0.0012 0.0029 0.0007 0.0006 0.0005 0.0001 0.0042 0.0001 0.0004 0.0001
Vacuole 0.0013 0.0003 0.0008 0.0003 0.0029 0.0006 0.0019 0.0014 0.0071 0.0004 0.0017 0.0029 0.0002 0.0001 0.0004 0 0.0005 0.0003
Vacuole Periphery 0.0011 0 0.0017 0.0004 0.0022 0.0002 0.0017 0.0001 0.0028 0.0002 0.002 0.0004 0 0 0.0001 0 0.0005 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1654 847 502 1017 1622 1816 1150 1438 3276 2663 1652 2455

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.45 675.42 857.22 749.86 648.60 716.78 766.42 762.92 632.37 703.62 794.01 757.51
Standard Deviation 59.59 88.26 86.27 103.45 67.32 91.32 81.68 118.96 65.54 92.38 93.00 112.98
Intensity Change Log 2 — 0.131801 0.475682 0.282637 — 0.144201 0.240806 0.234203 — 0.138172 0.360037 0.258008

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000087 0.000327 0.000587 0.000260 0.000207 0.000250 0.000190 0.000186 0.000146 0.000274 0.000311 0.000217
Bud Neck 0.034616 0.045641 0.002499 0.004717 0.027243 0.049794 0.002726 0.005697 0.030966 0.048473 0.002657 0.005291
Bud Site 0.004855 0.012170 0.002601 0.014966 0.004190 0.016004 0.003447 0.028068 0.004526 0.014784 0.003190 0.022640
Cell Periphery 0.000257 0.000163 0.000129 0.000306 0.000183 0.000199 0.000216 0.001754 0.000220 0.000188 0.000189 0.001154
Cytoplasm 0.446900* 0.313158* 0.134042* 0.457609* 0.391973* 0.365956* 0.383542* 0.464384* 0.419705* 0.349163* 0.307726* 0.461577*
Cytoplasmic Foci 0.247560* 0.327201* 0.008601 0.034655 0.313149* 0.267006* 0.021483 0.019783 0.280034* 0.286151* 0.017569 0.025944
Eisosomes 0.000372 0.000206 0.000066 0.000090 0.000271 0.000212 0.000069 0.000043 0.000322 0.000210 0.000068 0.000062
Endoplasmic Reticulum 0.001035 0.000427 0.005442 0.000771 0.001509 0.000532 0.000544 0.001520 0.001270 0.000498 0.002032 0.001210
Endosome 0.015040 0.034291 0.002518 0.002972 0.015921 0.027790 0.001300 0.002411 0.015476 0.029858 0.001671 0.002644
Golgi 0.003816 0.007936 0.000080 0.000567 0.005120 0.006709 0.000120 0.001181 0.004462 0.007099 0.000108 0.000927
Lipid Particles 0.015925 0.014613 0.002838 0.001961 0.017835 0.011440 0.001208 0.001119 0.016871 0.012449 0.001703 0.001468
Mitochondria 0.008149 0.025894 0.000509 0.000699 0.011892 0.015827 0.000971 0.001990 0.010002 0.019029 0.000831 0.001455
Mitotic Spindle 0.000458 0.007315 0.004131 0.004837 0.001761 0.006923 0.002179 0.012418 0.001103 0.007048 0.002772 0.009277
None 0.003581 0.004169 0.008659 0.020151 0.003139 0.002272 0.010832 0.005036 0.003362 0.002876 0.010172 0.011297
Nuclear Periphery 0.000532 0.000468 0.002519 0.000808 0.000677 0.000717 0.000895 0.001391 0.000604 0.000638 0.001388 0.001150
Nuclear Periphery Foci 0.000189 0.000393 0.000966 0.000509 0.000313 0.000300 0.000200 0.000540 0.000250 0.000330 0.000433 0.000527
Nucleolus 0.001384 0.002498 0.001306 0.000682 0.001318 0.002011 0.000818 0.000429 0.001352 0.002166 0.000967 0.000534
Nucleus 0.154907* 0.104632 0.774691* 0.391199* 0.152218* 0.121404 0.538540* 0.387198* 0.153576* 0.116069 0.610300* 0.388855*
Peroxisomes 0.006407 0.033170 0.002755 0.000989 0.011756 0.019228 0.001308 0.000397 0.009056 0.023662 0.001748 0.000642
Vacuole 0.052564 0.062657 0.044851 0.061074 0.038095 0.082740 0.029261 0.064017 0.045400 0.076352 0.033998 0.062798
Vacuole Periphery 0.001366 0.002672 0.000208 0.000179 0.001230 0.002688 0.000149 0.000439 0.001299 0.002683 0.000167 0.000332

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.21 -3.53 -2.92 0.99 2.19 -0.82 0.26 -0.11 1.07 -0.34 -3.94 -2.17 -2.58 1.21 1.03
Bud Neck -2.62 14.48 13.68 12.11 -1.10 -7.24 12.44 11.14 17.82 -3.79 -7.06 19.02 17.61 21.57 -3.68
Bud Site -5.36 2.50 -2.91 0.14 -3.73 -7.26 0.83 -5.25 -0.51 -5.35 -8.64 1.81 -5.95 -0.17 -6.40
Cell Periphery 5.42 7.82 1.15 -2.34 -3.13 -0.58 -0.51 -1.02 -1.00 -1.00 2.21 2.70 -0.97 -1.05 -1.07
Cytoplasm 12.49 34.53 4.44 -7.11 -25.01 2.86 3.33 1.42 -1.25 -1.90 10.31 16.85 4.55 -5.02 -11.82
Cytoplasmic Foci -8.43 46.27 40.60 36.98 -9.37 5.82 48.82 49.86 45.40 2.04 -0.97 65.29 64.08 57.58 -3.27
Eisosomes 6.33 11.43 11.65 11.13 0.72 3.92 14.72 17.67 15.99 2.22 7.30 17.03 18.39 18.77 1.68
Endoplasmic Reticulum 3.36 -13.59 -3.21 -10.83 12.81 3.00 2.08 -2.68 -13.71 -11.23 4.16 -7.17 -4.24 -17.12 4.84
Endosome -7.82 12.78 12.42 13.58 1.53 -6.87 13.66 12.71 18.66 -3.23 -10.50 19.43 18.09 22.91 -1.22
Golgi -3.30 5.67 5.02 7.35 -1.39 -1.85 9.49 6.93 8.06 -1.30 -3.70 10.30 8.14 10.99 -1.69
Lipid Particles 0.61 11.38 13.12 7.00 4.61 4.55 14.06 14.08 13.08 0.11 3.96 18.72 19.34 13.87 3.27
Mitochondria -5.48 6.32 6.12 8.54 -0.42 -1.99 7.34 6.95 10.73 -0.58 -5.58 9.38 9.09 13.77 -0.54
Mitotic Spindle -3.54 -1.78 -3.76 0.13 -1.36 -3.41 -0.66 -4.53 -1.28 -3.99 -5.15 -1.98 -6.07 -0.90 -4.02
None -0.49 -1.03 -4.78 -4.27 -2.76 1.52 -3.89 -1.58 -5.02 3.38 1.08 -3.88 -4.99 -5.72 0.04
Nuclear Periphery 0.34 -15.11 -6.42 -10.67 12.14 -0.28 -4.98 -12.41 -7.92 -9.21 -0.35 -12.40 -13.23 -9.72 -1.35
Nuclear Periphery Foci -2.21 -9.63 -4.83 -2.48 3.37 0.08 1.27 -5.26 -4.56 -6.14 -1.48 -7.32 -7.36 -5.10 -2.42
Nucleolus -3.66 0.93 5.30 7.02 4.32 -1.96 2.91 4.76 6.33 2.77 -3.47 3.42 7.12 8.77 4.36
Nucleus 7.45 -52.76 -26.68 -29.50 25.59 5.57 -41.98 -30.88 -35.37 12.15 8.98 -59.63 -40.96 -46.06 22.34
Peroxisomes -10.04 3.51 9.74 12.49 0.77 -4.78 8.82 11.25 16.15 1.17 -11.24 9.43 14.73 20.04 1.32
Vacuole -3.05 -3.77 -10.85 -8.27 -5.65 -13.14 -2.17 -18.18 -9.37 -15.59 -11.95 -2.98 -20.65 -11.91 -16.36
Vacuole Periphery -4.21 8.68 9.30 9.16 2.80 -5.63 10.38 7.95 9.60 -1.61 -6.78 13.05 11.73 12.87 -1.08
Standard name
Human Ortholog
Description Protein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fat3

Fat3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fat3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available