Standard name
Human Ortholog
Description Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 – 0.06 0 0.08 0.09 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 1.0 0.95 0.95 0.88 0.83 – 0.78 0.76 0.76 0.74 1.0 1.0 0.99 0.98 0.99 0.99 0.97 0.96 0.96 0.96 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 – 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0 – 0.09 0.07 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.05 0.05 0.1 – 0.1 0.16 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 4 2 9 6 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 0 1 0 – 1 4 2 5 0 0 0 0 0 0 0 0 0 0 0 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 6 8 5 4 3 22 – 22 14 43 41 1 7 6 14 7 5 0 0 0 0 0 0
Cytoplasm 254 363 256 179 226 199 287 – 308 339 386 331 247 364 275 262 236 176 247 341 243 232 339 311
Endoplasmic Reticulum 0 1 1 0 1 2 19 – 14 15 15 38 0 4 1 9 5 2 2 1 2 1 5 3
Endosome 0 0 0 0 0 0 0 – 0 1 1 1 0 0 0 0 0 0 0 3 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0
Mitochondria 1 1 0 2 1 17 2 – 36 29 44 36 0 0 0 2 1 1 0 0 1 0 1 0
Nucleus 0 2 0 0 1 2 4 – 6 0 6 4 0 0 2 1 2 3 0 2 1 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 2 0 13 12 12 33 – 41 71 53 31 1 0 0 3 1 0 0 4 0 1 0 0
Unique Cell Count 259 365 256 188 238 225 346 395 444 509 450 248 365 277 267 239 178 256 356 253 242 351 330
Labelled Cell Count 269 376 265 199 245 236 367 433 475 560 496 249 376 284 291 253 187 256 356 253 242 351 330


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.0 14.0 13.6 13.2 13.7 9.7 12.3 – 11.4 11.6 11.4 11.2 15.0 16.6 16.5 16.8 16.6 17.1 13.4 13.6 13.9
Std Deviation (1e-4) 2.1 2.4 1.8 2.1 2.0 2.2 2.2 – 2.3 2.1 2.2 2.3 2.6 2.6 2.6 4.3 3.1 3.4 3.7 2.2 2.3
Intensity Change (Log2) – – – -0.04 0.01 -0.49 -0.14 – -0.26 -0.23 -0.25 -0.29 0.14 0.29 0.28 0.3 0.28 0.33 -0.02 0.0 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 -1.0 -1.3 1.8 – 1.5 0 2.8 3.0 0 -1.0 -0.7 1.2 -0.1 -0.2
Cytoplasm -3.5 -3.6 -5.6 -7.0 – -8.1 -8.4 -8.6 -9.0 -1.0 -0.8 -1.4 -2.2 -1.8 -1.7
Endoplasmic Reticulum 0 0 0 3.5 – 2.6 2.5 2.3 4.5 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.5 0 – 5.0 4.2 4.8 4.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 4.3 3.6 3.7 5.1 – 5.3 6.7 5.4 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.4633 10.8386 10.1721 10.3301 9.5038 10.0927 12.8063 14.2376 13.2251 12.2435 12.3152 13.1464 15.4274 17.0985 16.1531 15.4337 14.8713 15.8098
Actin 0.0002 0.0002 0.0002 0.0001 0.0007 0.0003 0.0002 0.0002 0.0006 0.0002 0.0001 0.0012 0.0004 0.0004 0.0003 0.0003 0.0002 0.0003
Bud 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0007 0.0001 0 0.001 0 0.0001 0.0001 0.0001 0 0
Bud Neck 0.0002 0.0001 0.0002 0.0003 0.0004 0.001 0.0001 0.0002 0.0006 0.0003 0.0003 0.003 0.0002 0.0001 0.0002 0.0003 0.0003 0.0008
Bud Periphery 0 0 0 0 0 0.0001 0 0 0.0005 0 0 0.0003 0 0 0 0 0 0
Bud Site 0.0001 0.0004 0.0001 0 0.0001 0 0 0.0002 0.0032 0 0 0.0008 0.0001 0.0002 0.0001 0.0001 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.983 0.9888 0.9892 0.9805 0.9833 0.9844 0.9857 0.9891 0.9822 0.9544 0.9937 0.9754 0.9879 0.9939 0.9919 0.9891 0.9893 0.9854
Cytoplasmic Foci 0.002 0.0014 0.0011 0.0012 0.0034 0.0016 0.0009 0.0017 0.0014 0.0013 0.0009 0.0014 0.0015 0.001 0.001 0.0015 0.0011 0.0021
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0019 0.0021 0.0007 0.0043 0.0015 0.0017 0.0012 0.001 0.0009 0.0009 0.003 0.0016 0.0005 0.0008 0.0012 0.0011 0.0019
Endosome 0.0016 0.0015 0.0006 0.0051 0.0012 0.001 0.0005 0.0004 0.0003 0.0005 0.0003 0.0008 0.0009 0.0003 0.0004 0.0008 0.0003 0.001
Golgi 0.0001 0.0002 0 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001
Lipid Particles 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
None 0.0032 0.0012 0.0011 0.0007 0.0014 0.0009 0.0017 0.0008 0.0011 0.0008 0.0005 0.0008 0.0019 0.0011 0.0009 0.001 0.0007 0.001
Nuclear Periphery 0.0013 0.0008 0.0011 0.0009 0.001 0.0011 0.0016 0.0008 0.0007 0.0028 0.0005 0.0013 0.001 0.0002 0.0006 0.0007 0.0012 0.0008
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.0054 0.0026 0.0038 0.0071 0.0029 0.0067 0.0067 0.0043 0.0068 0.0379 0.0024 0.0099 0.0038 0.0016 0.0031 0.0041 0.005 0.006
Peroxisomes 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0002 0.0001 0.0002 0.0001 0.0005 0.0002 0.0002 0.0006 0.0003 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0.0003 0.0002
Vacuole 0.0006 0.0004 0.0003 0.0028 0.0002 0.0004 0.0003 0.0002 0.0002 0.0003 0.0003 0.0004 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002
Vacuole Periphery 0.0001 0.0001 0 0.0005 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.0774 35.4946 54.0633 58.0143 67.7373 37.5952 46.7496 68.8425 80.8542 66.3286
Translational Efficiency 3.3374 2.0272 1.5024 1.693 1.3723 3.1297 2.001 1.4887 1.6188 1.8113

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1748 910 2131 1884 1842 1977 257 1257 3590 2887 2388 3141

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1027.48 1331.73 1739.68 1547.67 1134.44 1290.59 1749.26 1601.98 1082.36 1303.56 1740.71 1569.40
Standard Deviation 151.69 214.30 236.60 231.26 157.33 177.63 223.75 294.65 163.59 190.91 235.27 259.87
Intensity Change Log 2 — 0.374191 0.759712 0.590988 — 0.186050 0.624764 0.497876 — 0.278531 0.690476 0.542878

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000208 0.000246 0.000134 0.000421 0.000082 0.000175 0.000583 0.003225 0.000143 0.000197 0.000183 0.001543
Bud Neck 0.000869 0.000631 0.000570 0.002083 0.000616 0.000947 0.002390 0.001847 0.000739 0.000847 0.000766 0.001989
Bud Site 0.008343 0.007871 0.002257 0.012325 0.003584 0.005177 0.010086 0.039832 0.005901 0.006026 0.003099 0.023333
Cell Periphery 0.000118 0.000102 0.000095 0.000087 0.000051 0.000047 0.000350 0.000242 0.000084 0.000064 0.000123 0.000149
Cytoplasm 0.822314* 0.901769* 0.865486* 0.814404* 0.893985* 0.899145* 0.808253* 0.811040* 0.859088* 0.899972* 0.859327* 0.813058*
Cytoplasmic Foci 0.103460 0.036925 0.006949 0.018687 0.044006 0.028776 0.029312 0.030044 0.072955 0.031345 0.009356 0.023232
Eisosomes 0.000012 0.000004 0.000001 0.000005 0.000005 0.000004 0.000006 0.000009 0.000008 0.000004 0.000002 0.000007
Endoplasmic Reticulum 0.001741 0.000355 0.000086 0.000480 0.000464 0.000141 0.000208 0.000345 0.001086 0.000209 0.000099 0.000426
Endosome 0.003157 0.001641 0.000240 0.005803 0.000759 0.001161 0.001301 0.007594 0.001927 0.001312 0.000354 0.006520
Golgi 0.003147 0.002616 0.000309 0.007398 0.001526 0.001874 0.000546 0.016604 0.002316 0.002108 0.000334 0.011082
Lipid Particles 0.000903 0.000514 0.000498 0.002378 0.000382 0.000390 0.002183 0.002956 0.000636 0.000429 0.000679 0.002609
Mitochondria 0.000450 0.000113 0.000064 0.000931 0.000164 0.000216 0.000027 0.004636 0.000303 0.000184 0.000060 0.002414
Mitotic Spindle 0.001347 0.001697 0.001620 0.014455 0.000189 0.002828 0.001424 0.016135 0.000753 0.002472 0.001599 0.015127
None 0.001640 0.003599 0.006776 0.001350 0.005044 0.007763 0.002467 0.001183 0.003387 0.006450 0.006312 0.001283
Nuclear Periphery 0.000051 0.000035 0.000092 0.000325 0.000028 0.000024 0.000160 0.000059 0.000039 0.000028 0.000100 0.000219
Nuclear Periphery Foci 0.001333 0.003772 0.001731 0.002133 0.000860 0.000847 0.008854 0.002658 0.001090 0.001769 0.002498 0.002343
Nucleolus 0.000463 0.000109 0.000078 0.000267 0.000135 0.000188 0.000070 0.000101 0.000295 0.000163 0.000078 0.000200
Nucleus 0.020431 0.022087 0.102989 0.030592 0.031227 0.033280 0.068909 0.014464 0.025970 0.029752 0.099321 0.024138
Peroxisomes 0.000468 0.000231 0.000048 0.000531 0.000230 0.000742 0.000100 0.002565 0.000346 0.000581 0.000053 0.001345
Vacuole 0.028240 0.014724 0.009910 0.082454 0.016274 0.015975 0.062668 0.039143 0.022100 0.015581 0.015588 0.065121
Vacuole Periphery 0.001305 0.000961 0.000067 0.002892 0.000388 0.000298 0.000103 0.005320 0.000834 0.000507 0.000071 0.003863

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.39 -1.05 -1.29 0.06 -0.56 -1.56 -9.06 -4.40 -4.12 -2.44 -1.41 -6.91 -4.61 -4.11 -3.64
Bud Neck 0.98 -2.76 -5.92 -5.70 -4.12 -2.12 -9.43 -11.95 -6.60 5.32 -1.19 -8.04 -11.07 -7.48 -3.69
Bud Site 0.03 8.69 0.02 -0.00 -5.94 -2.77 -9.26 -10.48 -9.66 -4.77 -0.43 3.07 -9.50 -8.91 -10.48
Cell Periphery 0.54 -0.65 1.85 4.49 6.61 1.42 -10.76 -13.87 -14.13 5.89 1.52 -6.10 -4.35 -11.51 3.00
Cytoplasm -11.54 -0.91 9.06 19.77 11.05 -0.92 13.22 13.00 13.37 -6.09 -10.68 10.70 16.99 25.48 7.10
Cytoplasmic Foci 16.30 27.84 25.44 9.27 -6.99 6.21 -0.19 6.65 0.49 4.96 17.77 29.09 24.73 7.54 -7.76
Eisosomes 7.73 10.34 7.63 -0.53 -7.37 1.30 -5.34 -3.53 -6.29 2.80 6.82 8.22 3.39 -5.29 -7.79
Endoplasmic Reticulum 5.04 5.98 5.57 2.05 -2.27 2.68 1.34 2.01 -0.83 1.35 5.95 6.50 5.88 -0.11 -1.87
Endosome 4.21 12.10 1.40 -1.90 -5.67 -1.80 -4.42 -5.40 -4.50 -3.73 3.04 11.47 -3.43 -4.92 -7.83
Golgi 0.77 15.75 0.92 -0.03 -3.27 -0.58 3.25 -3.90 -3.66 -4.48 0.71 12.13 -3.03 -3.11 -5.64
Lipid Particles 3.06 -1.06 -2.84 -3.81 -2.53 -0.31 -8.68 -8.48 -8.37 -0.33 2.66 -7.33 -7.68 -8.32 -5.10
Mitochondria 2.16 2.45 0.57 -1.55 -1.85 -0.70 6.72 -2.41 -2.35 -2.56 1.43 3.08 -2.18 -2.57 -2.89
Mitotic Spindle -0.46 -1.58 -3.48 -3.28 -2.90 -2.95 -5.00 -4.97 -3.08 -3.51 -2.35 -4.13 -6.14 -4.05 -4.41
None -3.27 -13.14 3.52 4.50 15.76 -3.93 7.47 10.95 12.17 4.40 -6.45 -6.27 11.25 12.92 16.42
Nuclear Periphery 1.06 -11.65 -4.22 -4.49 -1.02 0.75 -11.50 -7.06 -7.77 9.33 1.86 -17.18 -4.98 -5.34 1.14
Nuclear Periphery Foci -7.07 -5.77 -3.05 5.57 3.22 -0.14 -10.95 -7.71 -9.89 8.53 -3.88 -10.78 -7.66 -3.64 5.01
Nucleolus 1.50 1.60 1.03 -2.49 -7.62 -2.06 3.84 2.92 3.68 -0.64 1.11 1.78 1.09 -0.20 -7.04
Nucleus -1.86 -38.90 -12.01 -9.43 31.10 -2.09 -8.45 9.45 11.90 10.89 -4.69 -38.51 -4.34 0.16 36.09
Peroxisomes 4.04 10.25 3.26 -1.03 -5.91 -2.13 2.53 -2.77 -1.40 -2.94 -1.39 10.77 -2.26 -0.71 -3.95
Vacuole 5.07 1.13 -28.74 -30.83 -29.02 -0.69 -10.81 -17.87 -17.72 4.13 4.80 -7.52 -33.87 -35.76 -25.40
Vacuole Periphery 0.83 7.33 0.87 -0.25 -3.87 1.59 9.05 -3.01 -3.14 -3.39 2.10 9.05 -2.41 -3.26 -4.84
Standard name
Human Ortholog
Description Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Cnb1

Cnb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cnb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available