Standard name
Human Ortholog
Description Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 0.97 0.96 0.9 0.83 0.82 0.75 0.74 0.66 0.71 0.99 1.0 1.0 0.96 – – 0.93 0.95 0.92 0.97 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.06 0.05 0.22 0.26 0.24 0.35 0.43 0.5 0.46 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 0 0 1 1 1 1 0 0 0 0 – – 0 0 1 0 0 0
Bud 0 0 3 1 1 8 2 4 2 6 0 0 0 0 0 0 – – 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 9 9 2 5 8 4 9 8 9 13 5 5 2 6 10 2 – – 0 0 0 0 0 0
Cytoplasm 158 257 186 148 328 250 348 399 222 312 95 114 153 259 257 52 – – 176 257 180 139 175 147
Endoplasmic Reticulum 0 3 0 0 1 0 3 4 5 3 3 2 2 1 0 1 – – 3 2 2 1 2 2
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 – – 1 0 0 0 0 1
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – 1 1 0 0 0 0
Mitochondria 8 1 1 9 16 61 110 117 104 182 71 74 0 0 0 2 – – 0 1 1 0 1 1
Nucleus 0 3 1 1 0 1 3 3 0 1 0 0 0 0 1 1 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 1 0 3 0 0 0 0 0 0 – – 0 0 1 0 0 0
Nucleolus 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 1 0 2 1 3 1 4 13 4 8 1 3 0 1 0 0 – – 0 4 3 1 1 3
Unique Cell Count 162 265 190 153 342 277 421 488 295 423 143 160 155 260 258 54 190 270 196 144 187 158
Labelled Cell Count 177 274 195 165 359 326 481 552 347 530 176 199 157 267 268 58 190 270 196 144 187 158


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 10.1 7.7 7.0 6.5 5.3 5.0 5.4 4.8 4.7 4.4 4.5 9.7 9.1 8.9 13.8 – – 9.7 9.5 10.4
Std Deviation (1e-4) 1.1 1.4 1.1 1.4 1.0 1.2 1.2 1.2 0.9 1.0 0.9 1.2 0.9 1.2 1.2 2.8 – – 1.3 1.4 1.6
Intensity Change (Log2) – – – -0.14 -0.24 -0.53 -0.63 -0.52 -0.68 -0.7 -0.8 -0.76 0.33 0.24 0.21 0.84 – – 0.34 0.3 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1.8 0 – –
Cytoplasm -0.7 -1.2 -3.3 -5.3 -5.5 -6.7 -7.1 -7.8 -7.1 0.6 1.7 1.7 -0.7 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Mitochondria 0 2.6 6.7 7.6 7.2 0 0 0 0 0 0 0 0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6066 6.4362 5.763 4.9909 5.444 5.5905 4.6456 6.8596 5.5321 5.1585 4.7608 5.8665 6.0443 6.9046 7.3375 6.8675 6.4977 6.96
Actin 0.036 0.0017 0.0022 0.0006 0.0032 0.0004 0.0053 0.0038 0.0752 0.0091 0.0037 0.0038 0.0403 0.0003 0.0037 0.0006 0.0002 0.0118
Bud 0.0006 0.0005 0.0024 0.0006 0.0003 0.0022 0.0004 0.0003 0.0012 0.0003 0.0001 0.0002 0.0002 0.0003 0.0004 0.0003 0.0001 0.0003
Bud Neck 0.0031 0.0002 0.0007 0.0003 0.0005 0.0009 0.0002 0.0002 0.002 0.0003 0.0004 0.0005 0.0104 0.0002 0.0005 0.0002 0.0002 0.0009
Bud Periphery 0.0009 0.0002 0.0015 0.0001 0.0002 0.0008 0.0005 0.0003 0.0013 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0002
Bud Site 0.0061 0.0017 0.0079 0.0005 0.0006 0.0002 0.0006 0.0017 0.0078 0.0004 0.0003 0.0006 0.0039 0.0011 0.0051 0.0002 0 0.001
Cell Periphery 0.0009 0.0005 0.0004 0.0001 0.0001 0.001 0.0004 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0.0001 0 0.0001
Cytoplasm 0.7572 0.9572 0.9084 0.9243 0.8911 0.9641 0.7762 0.9321 0.6812 0.8403 0.8943 0.9269 0.6729 0.9733 0.8391 0.9712 0.9683 0.9039
Cytoplasmic Foci 0.0259 0.0052 0.0201 0.0152 0.0211 0.0075 0.0067 0.006 0.0299 0.037 0.0312 0.0149 0.0299 0.0069 0.0292 0.005 0.0029 0.0173
Eisosomes 0.0008 0 0.0001 0 0 0 0.0001 0.0001 0.0009 0 0 0 0.0001 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0087 0.006 0.0048 0.0062 0.0028 0.0055 0.0238 0.0032 0.0043 0.0308 0.007 0.0027 0.0109 0.0031 0.0046 0.0031 0.0012 0.0028
Endosome 0.0173 0.0099 0.0114 0.0338 0.0203 0.0075 0.0536 0.0061 0.0367 0.0323 0.0144 0.0089 0.0654 0.0085 0.013 0.0055 0.0017 0.0173
Golgi 0.0159 0.001 0.0018 0.0011 0.0105 0.0011 0.005 0.0049 0.0135 0.0104 0.0236 0.0009 0.027 0.001 0.0065 0.0003 0.0002 0.0172
Lipid Particles 0.0229 0.0002 0.0011 0.0001 0.0081 0.0001 0.0014 0.001 0.0216 0.008 0.0036 0.0007 0.0114 0.0001 0.0035 0.0001 0 0.0076
Mitochondria 0.0188 0.0003 0.012 0.0004 0.0209 0.001 0.0041 0.0326 0.0076 0.0008 0.0011 0.0002 0.005 0.0001 0.0012 0.0001 0.0001 0.0108
None 0.0485 0.0103 0.019 0.0026 0.0012 0.0045 0.1082 0.0046 0.0657 0.0126 0.0042 0.0372 0.0998 0.0019 0.0848 0.0092 0.0239 0.0016
Nuclear Periphery 0.0018 0.0013 0.0007 0.0011 0.0004 0.0004 0.0069 0.0003 0.0029 0.0026 0.0004 0.0003 0.0048 0.0004 0.0006 0.0007 0.0001 0.0003
Nucleolus 0.0036 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0033 0 0 0 0.0002 0 0.0001 0 0 0
Nucleus 0.0038 0.0008 0.0009 0.001 0.0006 0.0007 0.0012 0.0004 0.0058 0.0011 0.0004 0.0005 0.0013 0.0006 0.0006 0.0008 0.0004 0.0007
Peroxisomes 0.0167 0.0001 0.0006 0.0002 0.0151 0.0001 0.0002 0.0009 0.0207 0.0087 0.0142 0.0004 0.0107 0 0.0044 0 0 0.0044
Punctate Nuclear 0.0061 0.0002 0.0005 0.0002 0.0003 0.0001 0.0004 0.0001 0.0104 0.0014 0.0002 0.0004 0.0019 0.0001 0.0006 0.0002 0.0001 0.0003
Vacuole 0.0037 0.0024 0.0029 0.0098 0.0019 0.0014 0.0032 0.0008 0.0057 0.0032 0.0006 0.0007 0.0027 0.0016 0.0014 0.0019 0.0003 0.0011
Vacuole Periphery 0.0007 0.0004 0.0005 0.0016 0.0006 0.0003 0.0018 0.0004 0.0016 0.0005 0.0002 0.0001 0.0008 0.0002 0.0002 0.0003 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.9353 77.4351 52.4438 52.6538 57.0462 43.2872 51.2643 47.8108 42.0326 55.2999
Translational Efficiency 0.6587 0.5848 0.8142 0.6267 0.7013 0.7643 0.6254 0.5101 0.5529 0.5575

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2057 1648 97 1264 1959 2178 209 1409 4016 3826 306 2673

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 935.94 1010.26 1302.00 1222.77 957.18 1002.69 1364.62 1162.59 946.30 1005.95 1344.77 1191.05
Standard Deviation 114.67 144.59 144.69 163.27 116.26 141.68 158.88 163.42 115.93 142.99 157.25 166.09
Intensity Change Log 2 — 0.110239 0.476241 0.385665 — 0.067013 0.511637 0.280480 — 0.088545 0.494246 0.333441

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000161 0.000189 0.000662 0.001189 0.000167 0.000259 0.000455 0.000449 0.000164 0.000229 0.000520 0.000799
Bud Neck 0.002624 0.001396 0.002698 0.001778 0.002243 0.002095 0.001355 0.002515 0.002438 0.001794 0.001781 0.002166
Bud Site 0.011826 0.019984 0.017945 0.023965 0.014130 0.021505 0.015379 0.019693 0.012950 0.020850 0.016192 0.021713
Cell Periphery 0.000185 0.000098 0.000211 0.000109 0.000127 0.000087 0.000114 0.000070 0.000157 0.000092 0.000145 0.000088
Cytoplasm 0.793535* 0.790556* 0.460548* 0.786757* 0.728047* 0.754032* 0.757821* 0.680316* 0.761590* 0.769764* 0.663587* 0.730650*
Cytoplasmic Foci 0.104525 0.108550 0.114357 0.054056 0.167961 0.142991 0.065053 0.042570 0.135469 0.128156 0.080682 0.048001
Eisosomes 0.000026 0.000010 0.000045 0.000009 0.000030 0.000015 0.000013 0.000008 0.000028 0.000013 0.000023 0.000008
Endoplasmic Reticulum 0.001319 0.001091 0.006831 0.000188 0.001401 0.000926 0.000399 0.000329 0.001359 0.000997 0.002438 0.000262
Endosome 0.004643 0.008235 0.026942 0.005077 0.011536 0.009405 0.004737 0.010064 0.008005 0.008901 0.011776 0.007706
Golgi 0.008384 0.005434 0.000868 0.003211 0.008502 0.012142 0.000512 0.002064 0.008441 0.009253 0.000625 0.002606
Lipid Particles 0.001105 0.000640 0.004796 0.000970 0.001365 0.000864 0.001335 0.000758 0.001232 0.000768 0.002432 0.000858
Mitochondria 0.000914 0.001109 0.000064 0.000171 0.000508 0.002848 0.000016 0.000590 0.000716 0.002099 0.000031 0.000392
Mitotic Spindle 0.000203 0.002834 0.001101 0.012934 0.000459 0.003179 0.003233 0.015160 0.000328 0.003031 0.002557 0.014108
None 0.007338 0.000914 0.000128 0.000565 0.000972 0.000880 0.002225 0.000527 0.004233 0.000895 0.001560 0.000545
Nuclear Periphery 0.000025 0.000026 0.000673 0.000093 0.000021 0.000025 0.000112 0.000101 0.000023 0.000025 0.000290 0.000097
Nuclear Periphery Foci 0.000407 0.000426 0.014764 0.002009 0.000476 0.000290 0.005444 0.001033 0.000441 0.000349 0.008398 0.001494
Nucleolus 0.000323 0.000178 0.000167 0.000137 0.000136 0.000149 0.000071 0.000311 0.000232 0.000162 0.000101 0.000228
Nucleus 0.016258 0.007500 0.014802 0.011103 0.007556 0.005127 0.013664 0.013076 0.012013 0.006149 0.014025 0.012143
Peroxisomes 0.000641 0.000891 0.000768 0.001599 0.000950 0.001675 0.000424 0.002058 0.000792 0.001337 0.000533 0.001841
Vacuole 0.042550 0.045368 0.329650* 0.092977 0.050653 0.037672 0.127373 0.205186* 0.046502 0.040987 0.191494 0.152125
Vacuole Periphery 0.003008 0.004572 0.001981 0.001104 0.002761 0.003834 0.000266 0.003122 0.002887 0.004152 0.000810 0.002168

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.59 -4.43 -2.36 -2.28 -0.74 -2.14 -4.91 -2.47 -1.38 0.18 -1.86 -6.19 -3.04 -2.60 -0.85
Bud Neck 2.89 -0.13 1.95 -1.12 2.06 0.70 3.44 -0.25 -0.90 -3.43 2.66 1.90 1.59 -1.26 -0.45
Bud Site -5.96 -3.35 -4.02 0.37 -0.24 -5.67 -0.41 -1.73 2.73 -1.10 -8.46 -2.57 -4.21 2.15 -1.29
Cell Periphery 8.54 -0.60 8.34 0.08 4.43 6.15 1.37 7.10 2.99 4.42 10.85 1.54 11.07 2.08 5.72
Cytoplasm 0.49 12.48 5.14 4.20 -10.87 -3.65 1.42 6.89 9.70 2.36 -1.64 8.89 8.96 9.79 -5.20
Cytoplasmic Foci -0.91 -0.26 14.78 13.86 6.45 4.86 18.72 30.53 26.41 4.85 2.12 12.69 32.42 28.98 7.11
Eisosomes 8.69 -3.95 9.23 0.80 7.69 6.94 7.00 11.27 9.23 4.85 10.62 1.67 14.58 7.69 8.06
Endoplasmic Reticulum 0.69 -2.55 4.31 5.01 3.29 1.68 6.05 6.69 2.88 1.75 1.63 -1.17 7.12 5.09 3.33
Endosome -5.49 -6.92 1.53 5.80 7.16 2.57 9.42 3.44 1.69 -3.31 -1.68 -2.03 3.39 4.57 3.74
Golgi 5.60 25.60 10.73 5.03 -2.15 -3.78 21.35 14.59 11.43 -3.23 -1.38 33.42 17.89 12.25 -3.76
Lipid Particles 6.39 -3.95 -0.68 -5.87 3.85 4.07 -0.52 5.07 0.56 3.67 6.16 -3.87 3.44 -2.43 4.74
Mitochondria -0.50 15.77 12.44 2.59 -1.21 -3.34 6.72 0.73 3.46 -3.66 -3.20 15.36 6.02 4.22 -3.96
Mitotic Spindle -2.95 -3.16 -4.58 -2.73 -4.04 -3.26 -3.35 -5.21 -3.70 -2.99 -4.48 -3.79 -6.93 -4.59 -4.20
None 9.74 12.12 11.48 1.42 -4.75 0.47 -0.61 3.02 3.25 1.31 9.63 5.30 11.72 2.87 1.08
Nuclear Periphery -0.07 -4.04 -4.62 -4.56 3.47 -0.67 -8.06 -8.44 -6.73 4.09 -0.56 -6.44 -8.58 -7.71 4.65
Nuclear Periphery Foci -0.42 -6.39 -12.27 -11.99 5.41 1.75 -7.69 -3.22 -4.92 6.78 1.57 -9.55 -10.18 -11.74 8.00
Nucleolus 1.84 4.04 9.83 0.72 0.31 -0.63 4.53 -2.22 -1.83 -3.07 1.87 7.70 0.81 -0.82 -2.88
Nucleus 13.72 -0.07 2.85 -8.41 1.03 7.27 -6.41 -9.57 -13.15 2.44 16.41 -4.47 -4.38 -15.69 2.75
Peroxisomes -1.65 -1.05 -1.99 -0.72 -1.48 -3.02 4.46 -1.79 0.25 -3.54 -3.68 3.94 -2.64 -0.06 -4.03
Vacuole -1.16 -11.93 -18.49 -17.40 8.21 6.15 -9.90 -26.45 -28.53 -3.94 3.35 -14.49 -31.61 -32.75 3.36
Vacuole Periphery -3.18 2.57 9.35 7.77 1.64 -2.35 16.14 1.19 2.56 -3.80 -3.76 11.01 4.72 6.37 -2.46
Standard name
Human Ortholog
Description Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Dph2

Dph2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dph2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available