Standard name
Human Ortholog
Description Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm; deletion mutation extends replicative lifespan, as does exclusion of Los1p from the nucleus in response to caloric restriction

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.06 0.06 0.09 0.08 0.12 0.1 0 0.08 0.08 0.05 0.13 0.21 0.19 0.12 0.09 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.21
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.28 0.31 0.63 0.61 0.69 0.83 0.81 0.8 0.83 0 0 0.06 0 0 0 0 0 0.05 0 0 0
Nucleus 0.06 0.11 0.13 0.25 0.18 0.2 0.18 0.2 0.13 0.09 0.14 0.1 0.06 0.07 0.07 0.08 0.09 0.15 0.05 0.12 0 0.18 0.09 0
Nuclear Periphery 0.23 0.21 0.18 0.38 0.28 0.17 0.19 0.09 0.15 0.19 0.13 0.17 0.21 0.16 0.15 0.16 0.14 0.23 0.74 0.53 0.64 0.27 0.35 0.34
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.84 0.67 0.32 0.38 0.22 0.19 0.16 0.12 0.13 0.16 0.19 0.71 0.64 0.64 0.67 0.74 0.66 0.06 0.18 0.12 0.25 0.28 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1
Bud 0 1 2 1 1 6 2 12 4 4 4 8 0 0 0 0 0 0 1 10 7 8 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 1 4 3 3 4 2 4 5 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 17 19 17 30 23 43 43 8 25 16 15 22 37 35 8 4 9 2 4 8 4 7 6
Endoplasmic Reticulum 4 0 2 2 0 0 4 1 6 7 1 2 1 4 5 2 1 2 3 3 11 3 3 7
Endosome 0 0 3 1 14 3 2 2 3 2 0 1 1 8 4 2 1 1 5 13 15 10 30 45
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2 0 1 3 3
Mitochondria 1 3 42 74 107 177 223 310 206 257 156 252 2 1 10 2 1 0 1 3 21 4 7 3
Nucleus 9 34 44 66 64 57 65 91 33 30 27 31 9 13 13 5 4 9 9 39 17 25 20 9
Nuclear Periphery 36 62 58 101 98 49 69 38 37 59 25 52 35 28 28 10 6 14 134 174 267 39 79 74
Nucleolus 2 0 0 3 2 5 3 9 4 4 3 7 2 5 3 2 0 0 1 6 1 2 1 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 1 0 2 0 2 3 2 0 2 0 0 0 1 4 9 8 3 2 3
Vac/Vac Membrane 133 252 222 84 134 63 71 72 31 42 31 58 116 114 116 43 32 40 11 59 51 36 64 53
Unique Cell Count 158 300 331 266 349 283 368 447 248 317 196 302 163 179 181 64 43 61 181 333 418 145 230 221
Labelled Cell Count 193 371 392 350 451 388 485 583 336 434 270 433 188 213 214 76 49 76 181 333 418 145 230 221


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.9 8.0 5.6 5.4 5.1 4.5 4.1 4.1 3.6 3.5 3.5 3.4 7.3 7.4 6.3 9.7 10.2 10.9 8.4 8.5 9.1
Std Deviation (1e-4) 1.6 1.4 1.0 1.1 1.2 1.2 1.0 1.2 0.7 0.9 1.0 1.0 1.2 1.4 1.1 2.2 2.5 1.9 1.5 1.3 1.6
Intensity Change (Log2) -0.04 -0.14 -0.31 -0.43 -0.46 -0.65 -0.65 -0.66 -0.74 0.4 0.41 0.17 0.8 0.87 0.97 0.58 0.61 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 1.4 1.2 2.8 2.0 -1.4 1.1 1.1 -0.4 2.9 5.1 4.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.6 5.7 0 0 0 0 0 0 0 -4.2 -4.7 -2.6 -2.2 0 -2.9
Nucleus 3.6 1.8 2.3 1.6 2.6 0 -1.5 0.2 -1.2 -2.6 -2.1 -2.1 -1.2 -0.7 0.3
Nuclear Periphery 5.6 3.3 0 0 0 0 0 0 0 1.1 -0.5 -0.6 -0.4 -0.6 1.0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -8.6 -7.5 -11.1 -12.8 -14.5 -13.1 -13.9 -11.4 -12.1 0.9 -0.8 -0.7 0 1.0 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6824 8.2413 7.4834 7.629 6.0471 7.4623 7.4914 9.1493 8.2096 7.2793 8.0358 8.5149 8.8449 9.6916 9.0237 9.087 8.4961 8.7151
Actin 0.0833 0.0107 0.0081 0.0005 0.1296 0.0036 0.0437 0.0004 0.0118 0.0023 0.0354 0.0014 0.0264 0.0002 0.0155 0.0033 0.0034 0.0013
Bud 0.0006 0.0004 0.0002 0 0.0006 0.0001 0.0006 0 0.0001 0.0005 0.0004 0 0.0002 0.0112 0.0001 0.0001 0 0
Bud Neck 0.0018 0.0004 0.0002 0.0001 0.003 0.0005 0.0039 0.0001 0.0012 0.0019 0.001 0.0004 0.0033 0 0.0006 0.0004 0.0003 0.0003
Bud Periphery 0.0012 0.0003 0.0003 0 0.001 0.0005 0.0007 0 0.0001 0.0008 0.0012 0.0001 0.0005 0.0216 0.0005 0.0003 0.0001 0.0001
Bud Site 0.004 0.0035 0.0003 0 0.0019 0.0001 0.007 0 0.009 0.0024 0.0018 0.0001 0.0006 0.0013 0.0014 0.0001 0.0002 0.0001
Cell Periphery 0.0009 0.0001 0.0001 0 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0001 0 0.0004 0.0002 0.0018 0 0.0001 0
Cytoplasm 0.0079 0.0024 0.0019 0.0006 0.0011 0.0005 0.007 0.0007 0.005 0.008 0.0042 0.0059 0.0019 0.0013 0.0073 0.001 0.0015 0.0005
Cytoplasmic Foci 0.0405 0.0011 0.0034 0 0.0104 0.0001 0.0291 0 0.0109 0.0129 0.0131 0.0024 0.009 0.0001 0.0048 0 0.0302 0.0024
Eisosomes 0.0065 0.0002 0.0001 0 0.0005 0.0001 0.0006 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.017 0.0084 0.0048 0.0028 0.0069 0.0029 0.0052 0.0032 0.0121 0.0132 0.0064 0.0049 0.0148 0.0023 0.0088 0.0086 0.0147 0.0026
Endosome 0.0363 0.0011 0.0088 0.0005 0.0152 0.0033 0.0218 0.0005 0.0225 0.0436 0.0256 0.0113 0.0291 0.0004 0.0034 0.0016 0.0417 0.0015
Golgi 0.0183 0.0007 0.003 0 0.0102 0.001 0.0139 0 0.0063 0.0056 0.0042 0.0009 0.006 0 0.0028 0.0001 0.0081 0.0014
Lipid Particles 0.0241 0.0027 0.0053 0 0.0283 0.0004 0.0211 0 0.0155 0.0047 0.0094 0.0017 0.0046 0 0.0017 0.0001 0.0635 0.0012
Mitochondria 0.02 0.0012 0.0044 0.0005 0.0084 0.0095 0.005 0.0005 0.0009 0.0669 0.0053 0.0007 0.0024 0.0004 0.0011 0.0013 0.0009 0.0006
None 0.008 0.0002 0.0004 0.0001 0.0023 0.0002 0.0041 0.0001 0.0008 0.0115 0.0032 0.0008 0.001 0.0006 0.0056 0.0003 0.0004 0.0001
Nuclear Periphery 0.6076 0.8222 0.8285 0.8083 0.7037 0.8664 0.7181 0.8314 0.7998 0.7574 0.7386 0.8666 0.8304 0.8899 0.8403 0.8864 0.7408 0.8926
Nucleolus 0.0021 0.006 0.0003 0.0004 0.003 0.0006 0.0014 0.0004 0.0002 0.0007 0.0026 0.0006 0.0002 0.0003 0.0002 0.0005 0.0006 0.0003
Nucleus 0.0706 0.1211 0.1111 0.1752 0.034 0.0986 0.0719 0.1504 0.08 0.0459 0.1156 0.0905 0.0501 0.0512 0.0816 0.0773 0.034 0.0782
Peroxisomes 0.0212 0.0006 0.0007 0 0.0117 0.0001 0.0121 0 0.0011 0.0016 0.0111 0.0003 0.0032 0 0.0022 0 0.0291 0.0075
Punctate Nuclear 0.0157 0.0014 0.0021 0.0009 0.0074 0.0007 0.0236 0.0008 0.0018 0.0025 0.0096 0.0034 0.0023 0.0004 0.0024 0.0015 0.0103 0.0007
Vacuole 0.003 0.0013 0.0014 0.0005 0.0027 0.0013 0.0021 0.0006 0.004 0.0073 0.0035 0.0017 0.0041 0.0016 0.001 0.0013 0.0033 0.001
Vacuole Periphery 0.0091 0.0141 0.0148 0.0096 0.0179 0.0095 0.0071 0.011 0.0166 0.01 0.0077 0.0063 0.0092 0.017 0.0166 0.0158 0.0171 0.0077

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.916 28.9671 29.4724 41.2521 32.2678 23.7052 32.5346 31.6897 25.5839 41.0797
Translational Efficiency 0.6672 0.7986 0.8132 0.4715 0.5636 0.7189 0.6506 0.4536 0.641 0.4766

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm; deletion mutation extends replicative lifespan, as does exclusion of Los1p from the nucleus in response to caloric restriction
Localization
Cell Percentages nuclear periphery (82%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Los1

Los1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Los1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available