Standard name
Human Ortholog
Description Protein involved in 5' RNA end processing; substrates include mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0.06 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0.08 0 0 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.57 0.83 0.75 0.77 0.81 0.65 0.6 0.46 0.35 0.4 0.32 0.25 0.92 0.96 0.95 – 0.77 0.8 0.25 0.35 0.17 0.34 0.38 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0.07 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0 0.05 0.08 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.13 0.06 0.13 0.17 0.25 0.29 0.42 0.7 0.61 0.75 0.75 0 0 0 – 0.07 0 0.44 0.47 0.56 0.44 0.4 0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.11 0.07 0.05 0.09 0.14 0.11 0.06 0.12 0.07 0.09 0 0 0 – 0.07 0.07 0 0 0.05 0.06 0.05 0.05
Vac/Vac Membrane 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0.05 0.06 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 0 1 0 0 0 0 0 2 0 – 1 0 3 6 8 4 4 7
Bud 1 2 4 5 0 6 6 13 24 13 11 18 0 1 1 – 2 0 1 0 0 3 3 8
Bud Neck 1 7 18 5 14 15 14 17 8 8 1 9 5 4 3 – 2 1 2 2 2 1 5 5
Bud Site 0 0 1 0 1 0 0 0 9 6 4 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 2 3 1 0 1 1 3 0 4 2 2 0 2 1 – 5 3 0 2 2 2 2 2
Cytoplasm 68 160 155 160 148 231 202 142 148 109 73 72 271 379 468 – 113 108 52 79 43 100 129 98
Endoplasmic Reticulum 1 0 1 1 2 0 1 0 0 0 0 0 0 1 1 – 8 10 6 3 1 1 1 3
Endosome 7 0 2 9 0 17 8 5 0 3 1 0 5 2 8 – 3 7 17 7 17 8 6 13
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 1 0 6 – 4 2 3 0 2 0 0 1
Mitochondria 31 25 13 28 31 88 97 130 295 164 170 211 6 4 1 – 10 5 93 105 144 129 139 124
Nucleus 5 0 1 1 1 5 2 6 1 5 3 4 0 2 1 – 1 1 0 0 0 1 1 1
Nuclear Periphery 5 0 0 0 0 0 2 1 0 0 0 1 0 0 0 – 0 1 0 0 0 0 0 1
Nucleolus 1 0 1 0 0 1 1 4 2 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0
Peroxisomes 4 0 0 6 0 3 2 4 1 2 3 0 3 0 2 – 0 0 1 0 5 1 0 1
SpindlePole 4 4 23 15 9 33 47 34 26 32 16 26 7 9 12 – 10 9 6 4 12 16 17 14
Vac/Vac Membrane 13 2 3 1 1 10 9 11 3 5 1 2 3 1 2 – 8 7 12 4 4 12 18 11
Unique Cell Count 120 192 206 209 183 357 339 307 420 271 227 283 295 394 492 147 135 212 226 258 296 345 307
Labelled Cell Count 141 202 226 232 208 410 392 372 517 351 285 347 301 407 506 167 154 212 226 258 296 345 307


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.0 4.9 4.9 4.1 4.1 4.1 4.2 3.1 3.7 3.3 3.3 4.8 4.7 4.6 3.9 5.1 5.4 4.8 5.3 5.7
Std Deviation (1e-4) 0.7 0.3 1.6 3.1 1.4 1.5 2.0 1.5 0.8 2.0 1.0 0.9 1.4 0.9 1.5 1.1 2.0 1.8 1.1 1.6 2.2
Intensity Change (Log2) – – – 0.02 -0.24 -0.25 -0.23 -0.2 -0.66 -0.41 -0.54 -0.54 -0.03 -0.05 -0.07 -0.32 0.07 0.16 -0.01 0.11 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5145 -0.9341 -0.9907 -1.1896 -1.3462 -0.9855 3.6404 4.4948 4.3917 4.6035 4.5683 4.8827 1.8206 1.8342 1.5827 1.4163 1.7089 1.6591
Actin 0.0641 0.0289 0.019 0.0326 0.0033 0.0061 0.0117 0.0118 0.0074 0.0072 0.0307 0.0104 0.0213 0.019 0.0349 0.0108 0.0014 0.0188
Bud 0.0012 0.0057 0.0126 0.0055 0.0023 0.0009 0.0022 0.0049 0.0037 0.0051 0.0057 0.0017 0.0024 0.0028 0.0027 0.0019 0.0002 0.0009
Bud Neck 0.0048 0.002 0.0008 0.0022 0.0004 0.0069 0.0046 0.0029 0.0035 0.0046 0.0056 0.0128 0.0037 0.0015 0.0009 0.0066 0.0014 0.007
Bud Periphery 0.0028 0.0045 0.0123 0.0112 0.0146 0.0023 0.0057 0.0077 0.0101 0.0192 0.02 0.0054 0.0037 0.0132 0.0088 0.0038 0.0007 0.0042
Bud Site 0.0025 0.0251 0.0084 0.0146 0.0006 0.0016 0.0083 0.0457 0.0192 0.0032 0.006 0.0023 0.0065 0.0307 0.0046 0.0089 0.0003 0.0019
Cell Periphery 0.0011 0.0034 0.002 0.0007 0.0015 0.0007 0.0039 0.0021 0.0019 0.0037 0.0108 0.0023 0.0008 0.0053 0.0028 0.0024 0.002 0.0025
Cytoplasm 0.0906 0.1096 0.0827 0.0618 0.204 0.1014 0.1147 0.1653 0.1279 0.1354 0.1039 0.1449 0.0455 0.0747 0.1096 0.1527 0.1431 0.1022
Cytoplasmic Foci 0.1319 0.1719 0.1582 0.107 0.0625 0.1392 0.1621 0.1495 0.1524 0.0859 0.1809 0.1519 0.1287 0.1153 0.0873 0.1425 0.0848 0.127
Eisosomes 0.002 0.0032 0.0014 0.0008 0.0011 0.0004 0.0011 0.0007 0.0009 0.0012 0.0016 0.0007 0.0005 0.0028 0.0018 0.001 0.0003 0.0009
Endoplasmic Reticulum 0.0092 0.0045 0.0042 0.0057 0.011 0.0027 0.0059 0.0041 0.0046 0.003 0.0069 0.0029 0.0055 0.0047 0.0044 0.0085 0.0037 0.0145
Endosome 0.1189 0.1136 0.137 0.1768 0.0596 0.1348 0.1569 0.1051 0.1132 0.0918 0.126 0.1026 0.2225 0.0663 0.0647 0.1153 0.0291 0.0688
Golgi 0.0372 0.0337 0.0467 0.0301 0.0063 0.0358 0.0322 0.0371 0.029 0.0278 0.0323 0.0322 0.0408 0.0209 0.023 0.0202 0.0099 0.017
Lipid Particles 0.0522 0.0178 0.0292 0.033 0.0169 0.0446 0.0333 0.011 0.0084 0.0029 0.0232 0.0097 0.0735 0.0187 0.0317 0.0189 0.005 0.0267
Mitochondria 0.0962 0.0907 0.1572 0.2026 0.1907 0.2189 0.1469 0.1607 0.1691 0.1874 0.1569 0.1731 0.1368 0.0871 0.1288 0.0435 0.1219 0.0994
None 0.3174 0.3251 0.2855 0.2435 0.3966 0.2511 0.2352 0.2264 0.2721 0.3479 0.1935 0.2836 0.1797 0.5 0.4318 0.328 0.5322 0.4496
Nuclear Periphery 0.0104 0.0014 0.0016 0.0039 0.0025 0.0012 0.0042 0.0007 0.0015 0.0017 0.0088 0.0009 0.0151 0.0028 0.0041 0.0021 0.0006 0.0041
Nucleolus 0.0018 0.0012 0.0007 0.0023 0.0008 0.0006 0.0019 0.0016 0.0014 0.0011 0.0016 0.0009 0.0012 0.0011 0.001 0.0008 0.0005 0.0008
Nucleus 0.0048 0.0016 0.0013 0.0022 0.0024 0.0011 0.0054 0.002 0.0031 0.002 0.004 0.0013 0.0059 0.0013 0.0011 0.0018 0.0006 0.0015
Peroxisomes 0.0197 0.044 0.0238 0.0303 0.004 0.0363 0.0405 0.0433 0.0431 0.0396 0.056 0.0488 0.0815 0.0216 0.0435 0.0994 0.0558 0.0258
Punctate Nuclear 0.0218 0.0023 0.0022 0.0088 0.0012 0.0024 0.0035 0.0014 0.0027 0.0012 0.0093 0.0017 0.0073 0.0009 0.0017 0.0012 0.0037 0.001
Vacuole 0.0068 0.0074 0.0094 0.0152 0.013 0.0073 0.0158 0.0127 0.0198 0.0222 0.0128 0.007 0.0128 0.0073 0.0088 0.0249 0.002 0.0201
Vacuole Periphery 0.0028 0.0024 0.0039 0.0092 0.0046 0.0037 0.0037 0.0032 0.005 0.0058 0.0035 0.0028 0.0044 0.002 0.0019 0.0048 0.0007 0.0054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.0566 9.6704 4.4849 10.9943 7.0128 5.4059 14.9721 12.7861 12.7026 12.2214
Translational Efficiency 2.1492 1.2239 2.2335 1.1311 1.3886 2.28 0.8684 1.2829 1.4344 1.2576

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
337 950 2803 2026 2076 2432 1416 94 2413 3382 4219 2120

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 587.48 606.96 820.84 786.47 648.75 787.54 781.50 869.90 640.19 736.82 807.64 790.17
Standard Deviation 67.07 90.10 93.35 114.66 71.52 89.16 92.51 86.11 74.02 120.76 94.90 114.84
Intensity Change Log 2 — 0.047062 0.482561 0.420852 — 0.279691 0.268583 0.423187 — 0.173801 0.374233 0.422078

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000532 0.000836 0.000316 0.005640 0.000221 0.000900 0.000636 0.002986 0.000264 0.000882 0.000424 0.005522
Bud Neck 0.067008 0.059554 0.018681 0.051418 0.036079 0.080416 0.014705 0.018123 0.040399 0.074556 0.017346 0.049942
Bud Site 0.009940 0.033706 0.013042 0.066417 0.011897 0.028036 0.012491 0.034740 0.011624 0.029629 0.012857 0.065012
Cell Periphery 0.001585 0.000240 0.000092 0.000224 0.000129 0.000281 0.000305 0.000337 0.000332 0.000270 0.000164 0.000229
Cytoplasm 0.113721* 0.154597* 0.378046* 0.278039* 0.250014* 0.129765* 0.298140* 0.276182* 0.230980* 0.136740* 0.351228* 0.277957*
Cytoplasmic Foci 0.311595* 0.282129* 0.038639 0.111767 0.386323* 0.297440* 0.097128 0.170871 0.375887* 0.293139* 0.058269 0.114388
Eisosomes 0.001017 0.000193 0.000032 0.000148 0.000153 0.000303 0.000156 0.000200 0.000273 0.000272 0.000074 0.000150
Endoplasmic Reticulum 0.001298 0.001418 0.003314 0.003461 0.001392 0.001043 0.003408 0.003605 0.001379 0.001149 0.003345 0.003467
Endosome 0.029511 0.095663 0.004610 0.025934 0.025571 0.064024 0.013500 0.032494 0.026121 0.072911 0.007594 0.026225
Golgi 0.033268 0.034090 0.000502 0.008717 0.019463 0.037590 0.001530 0.005350 0.021391 0.036607 0.000847 0.008568
Lipid Particles 0.033200 0.018805 0.002115 0.006026 0.020975 0.020471 0.011613 0.010046 0.022682 0.020003 0.005303 0.006204
Mitochondria 0.244982* 0.112166* 0.004134 0.019783 0.082066 0.159328* 0.004988 0.002096 0.104819* 0.146080* 0.004421 0.018998
Mitotic Spindle 0.001452 0.011425 0.016618 0.068339 0.005610 0.008621 0.008225 0.041279 0.005030 0.009408 0.013801 0.067139
None 0.005409 0.008656 0.003547 0.003362 0.004299 0.001921 0.007013 0.003995 0.004454 0.003813 0.004710 0.003390
Nuclear Periphery 0.000326 0.000690 0.003939 0.002530 0.000562 0.000389 0.002621 0.001020 0.000529 0.000474 0.003496 0.002463
Nuclear Periphery Foci 0.000143 0.000582 0.000861 0.001686 0.000296 0.000259 0.001863 0.003733 0.000275 0.000349 0.001197 0.001777
Nucleolus 0.001363 0.008044 0.001904 0.003407 0.003159 0.002232 0.004823 0.003675 0.002908 0.003865 0.002884 0.003419
Nucleus 0.041446 0.048569 0.458901* 0.124815 0.089925 0.039135 0.428747* 0.143824* 0.083155 0.041785 0.448780* 0.125658
Peroxisomes 0.078475 0.068570 0.004829 0.043972 0.021846 0.061179 0.015087 0.029654 0.029755 0.063255 0.008272 0.043337
Vacuole 0.018102 0.049672 0.045194 0.169662 0.034878 0.060004 0.071925 0.213426* 0.032535 0.057101 0.054166 0.171602
Vacuole Periphery 0.005626 0.010394 0.000684 0.004655 0.005140 0.006664 0.001095 0.002366 0.005208 0.007712 0.000822 0.004553

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.15 1.97 -6.57 -6.03 -6.89 -6.02 -3.97 -3.17 -2.27 -2.33 -6.99 -4.50 -7.21 -6.13 -6.77
Bud Neck 1.02 6.36 2.72 2.98 -11.73 -12.92 9.65 3.89 11.70 -0.44 -11.32 11.06 -1.66 9.65 -12.74
Bud Site -8.67 -1.38 -14.23 -5.33 -13.28 -9.55 -0.10 -2.76 -0.44 -2.67 -12.13 -0.47 -14.33 -7.60 -13.83
Cell Periphery 4.74 5.26 4.91 2.28 -6.31 -4.89 -6.72 -2.82 -0.27 -0.10 1.36 4.13 3.21 2.87 -2.06
Cytoplasm -3.73 -24.02 -15.29 -13.20 10.86 18.61 -4.24 -0.22 -4.84 1.14 16.11 -16.17 -5.68 -20.93 8.69
Cytoplasmic Foci 1.85 21.55 16.41 24.21 -17.17 12.74 44.63 13.24 8.07 -4.05 13.19 61.05 47.50 39.14 -12.65
Eisosomes 5.51 6.59 5.89 5.30 -15.81 -10.84 -0.53 -1.71 4.14 -1.47 0.05 8.86 5.79 11.45 -9.51
Endoplasmic Reticulum -0.29 -9.13 -7.50 -6.42 -0.03 1.65 -10.34 -4.46 -5.09 0.33 1.23 -13.91 -9.03 -9.86 0.21
Endosome -11.75 8.67 2.81 15.06 -13.61 -15.54 7.66 -0.70 7.59 -3.55 -19.77 14.96 3.04 22.15 -11.62
Golgi 0.00 8.90 7.22 10.20 -6.45 -9.55 17.05 6.28 12.24 -1.11 -8.81 19.84 11.73 19.37 -6.19
Lipid Particles 3.97 9.51 8.31 8.25 -11.14 0.26 6.54 5.16 4.98 0.53 1.76 14.87 14.03 13.82 -2.17
Mitochondria 7.97 15.26 14.53 14.53 -9.52 -13.24 20.97 21.94 34.09* 3.13 -7.38 25.14 21.88 33.31 -9.23
Mitotic Spindle -5.37 -9.70 -16.12 -12.27 -11.09 -2.44 -1.96 -2.32 -2.12 -2.08 -3.97 -6.83 -15.14 -13.94 -12.24
None -1.22 0.98 1.19 3.61 0.92 3.26 -2.26 1.00 -1.15 2.65 0.73 0.16 2.08 1.38 2.58
Nuclear Periphery -2.22 -24.11 -10.16 -8.20 3.97 1.83 -13.38 -3.35 -4.20 7.39 0.65 -26.62 -9.71 -9.80 2.78
Nuclear Periphery Foci -6.50 -7.23 -6.62 -4.99 -3.31 0.63 -6.29 -4.90 -4.93 -2.24 -1.88 -8.86 -6.70 -6.36 -2.57
Nucleolus -5.98 -3.26 -5.27 3.87 -3.50 1.99 -2.69 0.13 -0.89 1.70 -2.03 -0.41 -1.51 0.54 -1.45
Nucleus -1.21 -53.90 -14.73 -16.33 46.11 13.73 -38.58 -2.34 -4.91 13.40 12.26 -67.69 -11.92 -22.68 50.19
Peroxisomes 1.18 11.43 6.18 7.27 -13.46 -16.19 3.87 -0.35 4.18 -1.21 -14.32 14.66 -2.21 9.51 -12.01
Vacuole -8.32 -12.24 -34.20 -28.29 -28.39 -9.38 -14.19 -8.97 -7.96 -6.77 -11.09 -16.43 -35.57 -29.80 -27.64
Vacuole Periphery -4.04 7.20 2.73 6.77 -5.14 -3.34 13.20 5.52 7.73 -1.57 -5.47 15.83 3.51 7.31 -5.00
Standard name
Human Ortholog
Description Protein involved in 5' RNA end processing; substrates include mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
Localization
Cell Percentages mitochondrion (25%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cbt1

Cbt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available