Standard name
Human Ortholog
Description WD-repeat protein involved in ubiquitin-mediated protein degradation; Ub-binding protein with a role in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.69 0.66 0.38 0.39 0.47 0.46 0.44 0.35 0.4 0.33 0.24 0.32 0.76 0.38 0.61 – – – 0.44 0.44 0.4 0.34 0.35 0.29
Endoplasmic Reticulum 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0 0 – – – 0.05 0 0 0 0.05 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.08 0.05 0.24 0.25 0.39 0.19 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.1 0.07 0.26 0.23 0.13 0.18 0.24 0.18 0.19 0.17 0.15 0.16 0.05 0 0 – – – 0.23 0.26 0.23 0.17 0.17 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.07
Vac/Vac Membrane 0.34 0.45 0.44 0.44 0.47 0.41 0.33 0.52 0.32 0.41 0.42 0.43 0.22 0.67 0.45 – – – 0.15 0.17 0.21 0.34 0.28 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 2 0 0 0 1 0 0 0 – – – 0 0 2 0 3 2
Bud 0 0 0 2 0 2 7 1 6 8 18 13 0 1 0 – – – 3 5 6 6 11 13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 – – – 1 3 5 0 4 7
Bud Site 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 0 7 2 1 3 3 5 3 3 1 3 1 1 0 – – – 0 0 0 0 0 0
Cytoplasm 96 187 148 114 177 267 289 198 195 150 116 175 164 96 180 – – – 78 155 208 51 88 75
Endoplasmic Reticulum 2 9 24 12 10 12 27 2 9 4 1 11 14 1 4 – – – 8 5 13 4 12 14
Endosome 0 1 0 0 2 2 1 1 1 0 0 6 0 8 2 – – – 6 9 7 2 4 17
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 2 0 0 0
Mitochondria 1 2 2 3 2 41 50 31 120 114 187 101 3 0 0 – – – 0 3 20 1 3 2
Nucleus 14 21 102 66 48 106 157 101 91 75 74 84 10 6 12 – – – 41 91 122 26 42 33
Nuclear Periphery 0 0 0 0 0 0 0 2 0 1 3 4 0 0 0 – – – 6 6 3 1 1 2
Nucleolus 0 0 0 0 0 0 0 2 0 1 1 1 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 1 1 0 1 0 2 1 0 0 0 1 0 0 0 – – – 1 4 9 0 1 17
Vac/Vac Membrane 48 127 173 128 176 234 216 293 156 185 206 230 48 168 135 – – – 26 60 113 51 72 64
Unique Cell Count 140 285 394 291 376 575 652 567 491 454 485 541 216 251 297 176 353 526 151 253 258
Labelled Cell Count 162 348 458 327 417 667 755 639 581 543 607 632 240 281 333 176 353 526 151 253 258


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.4 7.1 6.7 7.4 6.1 6.2 6.5 5.4 5.2 4.9 5.9 7.6 8.2 8.0 – – – 6.9 7.4 7.6
Std Deviation (1e-4) 1.0 1.0 0.7 0.9 1.1 0.9 0.9 1.0 0.8 0.9 0.8 0.9 1.1 1.3 1.3 – – – 1.0 1.5 1.5
Intensity Change (Log2) – – – -0.1 0.05 -0.22 -0.2 -0.13 -0.4 -0.46 -0.54 -0.29 0.1 0.2 0.16 – – – -0.06 0.04 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 3.9 3.1 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.4 2.7 2.7 2.1 -0.8 0.7 -1.4 -4.4 -1.7 9.1 0.2 6.0 – – –
Endoplasmic Reticulum -1.1 -2.3 -3.2 -1.4 -5.4 -3.3 -4.2 -5.2 -3.2 0.2 -3.7 -3.1 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 4.9 5.2 4.2 10.3 10.4 13.7 8.8 0 0 0 – – –
Nucleus -1.0 -4.6 -2.8 -0.7 -3.0 -2.6 -3.3 -3.9 -3.9 -6.5 -7.8 -7.7 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0.8 -1.0 -3.5 2.4 -3.7 -0.9 -0.4 -0.4 -5.3 5.7 0.4 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0546 3.6988 3.288 3.0266 2.5842 3.0798 4.0312 4.9743 4.2986 3.8181 4.2581 4.2799 3.4029 4.0165 3.3124 3.2742 3.0773 3.3819
Actin 0.0223 0.005 0.0016 0.0108 0.0267 0.0023 0.0406 0.0006 0.0042 0.0083 0.0447 0.0081 0.0238 0.0004 0.0056 0.0023 0.0003 0.0054
Bud 0.0006 0.0006 0.0004 0.0005 0.0018 0.0003 0.0008 0.0001 0.0003 0.0023 0.0013 0.0002 0.0006 0.0001 0.0002 0.0002 0.0001 0.0002
Bud Neck 0.0029 0.0008 0.0032 0.001 0.0037 0.0072 0.0129 0.0005 0.0012 0.0022 0.0015 0.0029 0.0027 0.0004 0.0004 0.0009 0.0012 0.0021
Bud Periphery 0.001 0.0003 0.0003 0.0005 0.0014 0.0008 0.002 0.0001 0.0004 0.0024 0.0015 0.0004 0.0013 0 0.0002 0.0001 0.0002 0.0003
Bud Site 0.0021 0.0079 0.002 0.0012 0.0172 0.0004 0.0036 0.0007 0.0006 0.0039 0.0064 0.0003 0.0015 0.0003 0.0003 0.0003 0.0008 0.0012
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0005 0 0.0001 0.0003 0.0002 0.0001 0.0003 0 0 0 0.0001 0
Cytoplasm 0.0778 0.1696 0.1148 0.1033 0.1385 0.0654 0.0693 0.1885 0.098 0.0449 0.0785 0.0615 0.0867 0.194 0.0686 0.0703 0.1069 0.0441
Cytoplasmic Foci 0.0118 0.0018 0.0071 0.0157 0.0422 0.0045 0.0248 0.0027 0.0025 0.0134 0.0108 0.0038 0.0098 0.0008 0.0009 0.0167 0.0077 0.0122
Eisosomes 0.0001 0 0 0 0.0003 0 0.0002 0 0 0 0.0003 0 0.0002 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0126 0.0074 0.0039 0.005 0.0094 0.0037 0.0134 0.0046 0.0036 0.0104 0.009 0.0053 0.0078 0.0105 0.0028 0.0052 0.0024 0.0068
Endosome 0.0295 0.0036 0.0084 0.0314 0.0305 0.0174 0.035 0.0033 0.0055 0.0433 0.0385 0.0173 0.0101 0.0027 0.0058 0.027 0.0284 0.0081
Golgi 0.008 0.0004 0.0048 0.0136 0.0223 0.0079 0.0124 0.0002 0.0063 0.0104 0.0208 0.0052 0.0052 0 0.0028 0.0154 0.0034 0.0018
Lipid Particles 0.0048 0.0003 0.0053 0.0038 0.0175 0.0013 0.0161 0.0002 0.009 0.005 0.026 0.0027 0.0085 0.0001 0.0014 0.0051 0.0183 0.0036
Mitochondria 0.0069 0.0011 0.0061 0.0058 0.0455 0.0096 0.0071 0.0003 0.0022 0.0069 0.0656 0.0036 0.011 0.0004 0.0112 0.0058 0.0009 0.0025
None 0.0064 0.0037 0.0122 0.0063 0.0513 0.0016 0.0019 0.0029 0.0006 0.0011 0.0027 0.0025 0.0035 0.0069 0.0007 0.0053 0.0347 0.0004
Nuclear Periphery 0.0837 0.0451 0.0457 0.0496 0.0355 0.0367 0.0533 0.0438 0.0522 0.0384 0.0665 0.0376 0.08 0.0644 0.0484 0.0522 0.0308 0.0481
Nucleolus 0.0035 0.0014 0.0016 0.0005 0.0032 0.0014 0.0015 0.0013 0.0019 0.0013 0.0012 0.0015 0.0009 0.0006 0.0006 0.0006 0.0024 0.0009
Nucleus 0.707 0.7374 0.7695 0.738 0.5145 0.8258 0.6645 0.7388 0.8026 0.7822 0.6007 0.8307 0.7159 0.7094 0.8447 0.7731 0.7485 0.8514
Peroxisomes 0.002 0.0013 0.0031 0.0042 0.0147 0.001 0.0222 0.0004 0.001 0.0106 0.0073 0.0012 0.0155 0 0.0007 0.0018 0.0019 0.0037
Punctate Nuclear 0.0074 0.0096 0.0055 0.004 0.0139 0.0093 0.0103 0.0083 0.0053 0.0085 0.011 0.0109 0.0108 0.0059 0.003 0.0158 0.0056 0.0046
Vacuole 0.0065 0.0022 0.0033 0.0036 0.0069 0.0026 0.0058 0.0021 0.0016 0.003 0.0036 0.0029 0.0028 0.0023 0.0011 0.0012 0.0046 0.002
Vacuole Periphery 0.003 0.0005 0.0009 0.0009 0.0027 0.0007 0.0017 0.0004 0.0007 0.0013 0.002 0.0012 0.0011 0.0006 0.0006 0.0007 0.0008 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3455 29.3892 20.1043 29.5594 19.6476 23.6166 26.3492 29.1437 33.0428 30.3963
Translational Efficiency 1.5358 0.8815 1.3435 1.2782 1.4097 1.6246 1.1498 1.1692 1.1351 1.0419

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
294 1275 330 102 1740 786 262 91 2034 2061 592 193

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 827.90 890.36 1430.14 1216.23 814.75 885.60 1288.88 1215.77 816.65 888.54 1367.62 1216.01
Standard Deviation 812.65 130.55 173.62 136.87 109.05 107.13 142.87 159.40 325.04 122.18 175.38 147.92
Intensity Change Log 2 — 0.104932 0.788628 0.554888 — 0.120298 0.661689 0.577441 — 0.112574 0.727062 0.566118

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000209 0.000396 0.001871 0.002206 0.000176 0.000785 0.001646 0.001105 0.000181 0.000545 0.001772 0.001687
Bud Neck 0.003289 0.003531 0.012355 0.006914 0.001878 0.003245 0.008136 0.006248 0.002082 0.003422 0.010488 0.006600
Bud Site 0.002444 0.004322 0.003341 0.024096 0.001603 0.005367 0.004362 0.021886 0.001725 0.004721 0.003793 0.023054
Cell Periphery 0.000212 0.000136 0.000296 0.000262 0.000122 0.000163 0.000273 0.000272 0.000135 0.000146 0.000286 0.000267
Cytoplasm 0.469939* 0.499083* 0.044103 0.169408* 0.451317* 0.496462* 0.072413 0.259098* 0.454008* 0.498084* 0.056632 0.211697*
Cytoplasmic Foci 0.172277 0.073726 0.000634 0.057864 0.045791 0.089588 0.000877 0.021952 0.064073 0.079775 0.000742 0.040931
Eisosomes 0.000118 0.000051 0.000033 0.000046 0.000037 0.000056 0.000058 0.000063 0.000049 0.000053 0.000044 0.000054
Endoplasmic Reticulum 0.004989 0.010472 0.011328 0.008597 0.012436 0.006818 0.016683 0.015636 0.011359 0.009079 0.013698 0.011916
Endosome 0.005729 0.007752 0.001451 0.043575 0.003117 0.006743 0.002173 0.030693 0.003494 0.007367 0.001771 0.037501
Golgi 0.000552 0.001525 0.000180 0.190988* 0.000587 0.002290 0.000163 0.031677 0.000582 0.001817 0.000173 0.115873*
Lipid Particles 0.015486 0.006624 0.001389 0.002399 0.005227 0.007398 0.001338 0.003007 0.006710 0.006919 0.001366 0.002686
Mitochondria 0.002682 0.001974 0.002272 0.054269 0.002060 0.003321 0.001754 0.001447 0.002150 0.002488 0.002043 0.029363
Mitotic Spindle 0.000724 0.002452 0.001536 0.108534* 0.001541 0.003595 0.006053 0.009993 0.001423 0.002888 0.003535 0.062071
None 0.009345 0.006414 0.002922 0.005945 0.007178 0.003758 0.004352 0.031213 0.007491 0.005401 0.003555 0.017859
Nuclear Periphery 0.001743 0.003547 0.005197 0.002910 0.006106 0.004181 0.003616 0.002993 0.005475 0.003789 0.004497 0.002949
Nuclear Periphery Foci 0.006402 0.012594 0.001794 0.003192 0.012080 0.008419 0.002224 0.005590 0.011259 0.011002 0.001984 0.004323
Nucleolus 0.001201 0.001792 0.000608 0.000976 0.001870 0.001842 0.000807 0.000263 0.001773 0.001811 0.000696 0.000640
Nucleus 0.262959* 0.310741* 0.868765* 0.180019* 0.420015* 0.306480* 0.788658* 0.283288* 0.397314* 0.309116* 0.833312* 0.228710*
Peroxisomes 0.002655 0.001728 0.000221 0.002138 0.000642 0.002503 0.000178 0.001653 0.000933 0.002023 0.000202 0.001909
Vacuole 0.036580 0.050429 0.039498 0.094385 0.025679 0.046324 0.084008 0.262157* 0.027255 0.048864 0.059197 0.173490
Vacuole Periphery 0.000465 0.000710 0.000208 0.041278 0.000539 0.000662 0.000229 0.009767 0.000528 0.000692 0.000217 0.026420

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.62 -7.59 -2.74 -2.11 3.24 -2.01 -13.53 -7.02 -1.43 4.35 -3.18 -11.65 -5.08 -2.82 4.54
Bud Neck -0.39 -7.76 -4.54 -3.99 5.38 -3.01 -10.70 -6.45 -4.57 1.87 -3.18 -11.23 -8.81 -5.97 5.59
Bud Site -1.98 -4.50 -3.78 -2.94 -2.20 -2.33 -8.00 -5.16 -2.33 -2.37 -3.64 -9.07 -6.19 -3.88 -3.13
Cell Periphery 3.36 -6.11 0.16 -2.93 6.09 -0.95 -9.06 -4.62 -2.02 1.80 -0.68 -11.97 -5.06 -3.56 5.90
Cytoplasm -1.31 29.48 15.81 20.69 -6.78 -3.69 45.29 14.62 15.81 -4.78 -4.80 60.10 22.74 25.69 -8.15
Cytoplasmic Foci 9.17 16.74 15.40 14.40 -2.85 -8.52 21.83 15.58 16.95 -3.83 -3.98 25.39 18.98 22.10 -4.23
Eisosomes 6.00 6.09 4.79 -1.38 -1.56 -4.96 -8.71 -5.51 -2.61 0.79 -1.65 -4.57 -4.02 -2.92 -0.56
Endoplasmic Reticulum -5.06 -6.52 -1.21 2.46 3.92 5.17 -4.12 1.95 -1.10 4.68 2.54 -3.81 2.89 1.14 5.97
Endosome -1.82 5.78 -0.32 0.57 -2.06 -5.80 1.94 -0.84 1.01 -1.13 -6.05 4.59 -1.49 1.12 -2.31
Golgi -2.55 2.48 -1.88 -1.78 -1.91 -2.00 2.85 -0.83 -0.13 -0.99 -3.03 2.90 -2.00 -1.77 -2.06
Lipid Particles 2.94 4.41 4.18 5.61 -1.24 -2.30 7.24 2.94 3.97 -3.31 -0.41 7.52 5.51 6.68 -2.90
Mitochondria 0.38 -0.10 -0.85 -1.17 -0.92 -1.01 0.08 1.90 1.97 5.51 -0.44 -0.66 -0.81 -0.66 -0.66
Mitotic Spindle -2.34 -2.87 -4.10 -3.98 -3.89 -1.18 -3.02 -2.82 -2.61 -1.76 -1.69 -3.55 -4.91 -4.75 -4.24
None 0.90 2.14 1.01 0.29 -2.95 3.85 4.56 -0.73 -1.67 -1.85 2.10 5.41 -0.16 -1.28 -2.72
Nuclear Periphery -4.82 -7.25 -5.80 -2.66 1.09 2.10 1.48 2.65 -0.30 1.55 2.93 -0.43 1.06 -2.18 1.73
Nuclear Periphery Foci -3.66 2.13 1.21 6.41 -1.58 1.89 8.77 5.81 3.03 -1.76 0.08 8.69 6.72 6.87 -2.33
Nucleolus -2.05 1.11 3.54 3.74 2.51 -0.56 3.22 3.92 4.36 1.35 -0.63 3.21 4.43 5.45 2.70
Nucleus -3.35 -31.75 -1.51 0.19 17.39 10.89 -17.97 3.74 -0.49 13.08 11.13 -34.03 4.29 -0.16 21.78
Peroxisomes 1.30 3.68 3.42 3.91 -0.50 -3.85 5.13 2.48 4.47 -1.11 -3.95 5.45 3.65 6.03 -0.91
Vacuole -4.07 -5.27 -11.14 -10.54 -9.64 -6.59 -9.97 -12.92 -12.07 -8.27 -10.27 -11.84 -17.13 -15.86 -12.80
Vacuole Periphery -1.61 1.75 -1.39 -1.31 -1.46 -0.59 2.75 -0.48 -0.32 -0.90 -1.22 2.80 -1.45 -1.34 -1.62
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description WD-repeat protein involved in ubiquitin-mediated protein degradation; Ub-binding protein with a role in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
Localization
Cell Percentages cytoplasm (33%), ER (14%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Doa1

Doa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Doa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available