Standard name | |
---|---|
Human Ortholog | |
Description | WD-repeat protein involved in ubiquitin-mediated protein degradation; Ub-binding protein with a role in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 3 | 2 |
Bud | 0 | 0 | 0 | 2 | 0 | 2 | 7 | 1 | 6 | 8 | 18 | 13 | 0 | 1 | 0 | – | – | – | 3 | 5 | 6 | 6 | 11 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 1 | 3 | 5 | 0 | 4 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 7 | 2 | 1 | 3 | 3 | 5 | 3 | 3 | 1 | 3 | 1 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 96 | 187 | 148 | 114 | 177 | 267 | 289 | 198 | 195 | 150 | 116 | 175 | 164 | 96 | 180 | – | – | – | 78 | 155 | 208 | 51 | 88 | 75 |
Endoplasmic Reticulum | 2 | 9 | 24 | 12 | 10 | 12 | 27 | 2 | 9 | 4 | 1 | 11 | 14 | 1 | 4 | – | – | – | 8 | 5 | 13 | 4 | 12 | 14 |
Endosome | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 6 | 0 | 8 | 2 | – | – | – | 6 | 9 | 7 | 2 | 4 | 17 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 2 | 0 | 0 | 0 |
Mitochondria | 1 | 2 | 2 | 3 | 2 | 41 | 50 | 31 | 120 | 114 | 187 | 101 | 3 | 0 | 0 | – | – | – | 0 | 3 | 20 | 1 | 3 | 2 |
Nucleus | 14 | 21 | 102 | 66 | 48 | 106 | 157 | 101 | 91 | 75 | 74 | 84 | 10 | 6 | 12 | – | – | – | 41 | 91 | 122 | 26 | 42 | 33 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 4 | 0 | 0 | 0 | – | – | – | 6 | 6 | 3 | 1 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 1 | 4 | 9 | 0 | 1 | 17 |
Vac/Vac Membrane | 48 | 127 | 173 | 128 | 176 | 234 | 216 | 293 | 156 | 185 | 206 | 230 | 48 | 168 | 135 | – | – | – | 26 | 60 | 113 | 51 | 72 | 64 |
Unique Cell Count | 140 | 285 | 394 | 291 | 376 | 575 | 652 | 567 | 491 | 454 | 485 | 541 | 216 | 251 | 297 | 176 | 353 | 526 | 151 | 253 | 258 | |||
Labelled Cell Count | 162 | 348 | 458 | 327 | 417 | 667 | 755 | 639 | 581 | 543 | 607 | 632 | 240 | 281 | 333 | 176 | 353 | 526 | 151 | 253 | 258 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 7.4 | 7.1 | 6.7 | 7.4 | 6.1 | 6.2 | 6.5 | 5.4 | 5.2 | 4.9 | 5.9 | 7.6 | 8.2 | 8.0 | – | – | – | 6.9 | 7.4 | 7.6 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.7 | 0.9 | 1.1 | 0.9 | 0.9 | 1.0 | 0.8 | 0.9 | 0.8 | 0.9 | 1.1 | 1.3 | 1.3 | – | – | – | 1.0 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.05 | -0.22 | -0.2 | -0.13 | -0.4 | -0.46 | -0.54 | -0.29 | 0.1 | 0.2 | 0.16 | – | – | – | -0.06 | 0.04 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 3.1 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0.4 | 2.7 | 2.7 | 2.1 | -0.8 | 0.7 | -1.4 | -4.4 | -1.7 | 9.1 | 0.2 | 6.0 | – | – | – |
Endoplasmic Reticulum | -1.1 | -2.3 | -3.2 | -1.4 | -5.4 | -3.3 | -4.2 | -5.2 | -3.2 | 0.2 | -3.7 | -3.1 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 4.9 | 5.2 | 4.2 | 10.3 | 10.4 | 13.7 | 8.8 | 0 | 0 | 0 | – | – | – |
Nucleus | -1.0 | -4.6 | -2.8 | -0.7 | -3.0 | -2.6 | -3.3 | -3.9 | -3.9 | -6.5 | -7.8 | -7.7 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0.8 | -1.0 | -3.5 | 2.4 | -3.7 | -0.9 | -0.4 | -0.4 | -5.3 | 5.7 | 0.4 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0546 | 3.6988 | 3.288 | 3.0266 | 2.5842 | 3.0798 | 4.0312 | 4.9743 | 4.2986 | 3.8181 | 4.2581 | 4.2799 | 3.4029 | 4.0165 | 3.3124 | 3.2742 | 3.0773 | 3.3819 |
Actin | 0.0223 | 0.005 | 0.0016 | 0.0108 | 0.0267 | 0.0023 | 0.0406 | 0.0006 | 0.0042 | 0.0083 | 0.0447 | 0.0081 | 0.0238 | 0.0004 | 0.0056 | 0.0023 | 0.0003 | 0.0054 |
Bud | 0.0006 | 0.0006 | 0.0004 | 0.0005 | 0.0018 | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.0023 | 0.0013 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.0029 | 0.0008 | 0.0032 | 0.001 | 0.0037 | 0.0072 | 0.0129 | 0.0005 | 0.0012 | 0.0022 | 0.0015 | 0.0029 | 0.0027 | 0.0004 | 0.0004 | 0.0009 | 0.0012 | 0.0021 |
Bud Periphery | 0.001 | 0.0003 | 0.0003 | 0.0005 | 0.0014 | 0.0008 | 0.002 | 0.0001 | 0.0004 | 0.0024 | 0.0015 | 0.0004 | 0.0013 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0003 |
Bud Site | 0.0021 | 0.0079 | 0.002 | 0.0012 | 0.0172 | 0.0004 | 0.0036 | 0.0007 | 0.0006 | 0.0039 | 0.0064 | 0.0003 | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0008 | 0.0012 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0778 | 0.1696 | 0.1148 | 0.1033 | 0.1385 | 0.0654 | 0.0693 | 0.1885 | 0.098 | 0.0449 | 0.0785 | 0.0615 | 0.0867 | 0.194 | 0.0686 | 0.0703 | 0.1069 | 0.0441 |
Cytoplasmic Foci | 0.0118 | 0.0018 | 0.0071 | 0.0157 | 0.0422 | 0.0045 | 0.0248 | 0.0027 | 0.0025 | 0.0134 | 0.0108 | 0.0038 | 0.0098 | 0.0008 | 0.0009 | 0.0167 | 0.0077 | 0.0122 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0126 | 0.0074 | 0.0039 | 0.005 | 0.0094 | 0.0037 | 0.0134 | 0.0046 | 0.0036 | 0.0104 | 0.009 | 0.0053 | 0.0078 | 0.0105 | 0.0028 | 0.0052 | 0.0024 | 0.0068 |
Endosome | 0.0295 | 0.0036 | 0.0084 | 0.0314 | 0.0305 | 0.0174 | 0.035 | 0.0033 | 0.0055 | 0.0433 | 0.0385 | 0.0173 | 0.0101 | 0.0027 | 0.0058 | 0.027 | 0.0284 | 0.0081 |
Golgi | 0.008 | 0.0004 | 0.0048 | 0.0136 | 0.0223 | 0.0079 | 0.0124 | 0.0002 | 0.0063 | 0.0104 | 0.0208 | 0.0052 | 0.0052 | 0 | 0.0028 | 0.0154 | 0.0034 | 0.0018 |
Lipid Particles | 0.0048 | 0.0003 | 0.0053 | 0.0038 | 0.0175 | 0.0013 | 0.0161 | 0.0002 | 0.009 | 0.005 | 0.026 | 0.0027 | 0.0085 | 0.0001 | 0.0014 | 0.0051 | 0.0183 | 0.0036 |
Mitochondria | 0.0069 | 0.0011 | 0.0061 | 0.0058 | 0.0455 | 0.0096 | 0.0071 | 0.0003 | 0.0022 | 0.0069 | 0.0656 | 0.0036 | 0.011 | 0.0004 | 0.0112 | 0.0058 | 0.0009 | 0.0025 |
None | 0.0064 | 0.0037 | 0.0122 | 0.0063 | 0.0513 | 0.0016 | 0.0019 | 0.0029 | 0.0006 | 0.0011 | 0.0027 | 0.0025 | 0.0035 | 0.0069 | 0.0007 | 0.0053 | 0.0347 | 0.0004 |
Nuclear Periphery | 0.0837 | 0.0451 | 0.0457 | 0.0496 | 0.0355 | 0.0367 | 0.0533 | 0.0438 | 0.0522 | 0.0384 | 0.0665 | 0.0376 | 0.08 | 0.0644 | 0.0484 | 0.0522 | 0.0308 | 0.0481 |
Nucleolus | 0.0035 | 0.0014 | 0.0016 | 0.0005 | 0.0032 | 0.0014 | 0.0015 | 0.0013 | 0.0019 | 0.0013 | 0.0012 | 0.0015 | 0.0009 | 0.0006 | 0.0006 | 0.0006 | 0.0024 | 0.0009 |
Nucleus | 0.707 | 0.7374 | 0.7695 | 0.738 | 0.5145 | 0.8258 | 0.6645 | 0.7388 | 0.8026 | 0.7822 | 0.6007 | 0.8307 | 0.7159 | 0.7094 | 0.8447 | 0.7731 | 0.7485 | 0.8514 |
Peroxisomes | 0.002 | 0.0013 | 0.0031 | 0.0042 | 0.0147 | 0.001 | 0.0222 | 0.0004 | 0.001 | 0.0106 | 0.0073 | 0.0012 | 0.0155 | 0 | 0.0007 | 0.0018 | 0.0019 | 0.0037 |
Punctate Nuclear | 0.0074 | 0.0096 | 0.0055 | 0.004 | 0.0139 | 0.0093 | 0.0103 | 0.0083 | 0.0053 | 0.0085 | 0.011 | 0.0109 | 0.0108 | 0.0059 | 0.003 | 0.0158 | 0.0056 | 0.0046 |
Vacuole | 0.0065 | 0.0022 | 0.0033 | 0.0036 | 0.0069 | 0.0026 | 0.0058 | 0.0021 | 0.0016 | 0.003 | 0.0036 | 0.0029 | 0.0028 | 0.0023 | 0.0011 | 0.0012 | 0.0046 | 0.002 |
Vacuole Periphery | 0.003 | 0.0005 | 0.0009 | 0.0009 | 0.0027 | 0.0007 | 0.0017 | 0.0004 | 0.0007 | 0.0013 | 0.002 | 0.0012 | 0.0011 | 0.0006 | 0.0006 | 0.0007 | 0.0008 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.3455 | 29.3892 | 20.1043 | 29.5594 | 19.6476 | 23.6166 | 26.3492 | 29.1437 | 33.0428 | 30.3963 |
Translational Efficiency | 1.5358 | 0.8815 | 1.3435 | 1.2782 | 1.4097 | 1.6246 | 1.1498 | 1.1692 | 1.1351 | 1.0419 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
294 | 1275 | 330 | 102 | 1740 | 786 | 262 | 91 | 2034 | 2061 | 592 | 193 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 827.90 | 890.36 | 1430.14 | 1216.23 | 814.75 | 885.60 | 1288.88 | 1215.77 | 816.65 | 888.54 | 1367.62 | 1216.01 |
Standard Deviation | 812.65 | 130.55 | 173.62 | 136.87 | 109.05 | 107.13 | 142.87 | 159.40 | 325.04 | 122.18 | 175.38 | 147.92 |
Intensity Change Log 2 | — | 0.104932 | 0.788628 | 0.554888 | — | 0.120298 | 0.661689 | 0.577441 | — | 0.112574 | 0.727062 | 0.566118 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000209 | 0.000396 | 0.001871 | 0.002206 | 0.000176 | 0.000785 | 0.001646 | 0.001105 | 0.000181 | 0.000545 | 0.001772 | 0.001687 |
Bud Neck | 0.003289 | 0.003531 | 0.012355 | 0.006914 | 0.001878 | 0.003245 | 0.008136 | 0.006248 | 0.002082 | 0.003422 | 0.010488 | 0.006600 |
Bud Site | 0.002444 | 0.004322 | 0.003341 | 0.024096 | 0.001603 | 0.005367 | 0.004362 | 0.021886 | 0.001725 | 0.004721 | 0.003793 | 0.023054 |
Cell Periphery | 0.000212 | 0.000136 | 0.000296 | 0.000262 | 0.000122 | 0.000163 | 0.000273 | 0.000272 | 0.000135 | 0.000146 | 0.000286 | 0.000267 |
Cytoplasm | 0.469939* | 0.499083* | 0.044103 | 0.169408* | 0.451317* | 0.496462* | 0.072413 | 0.259098* | 0.454008* | 0.498084* | 0.056632 | 0.211697* |
Cytoplasmic Foci | 0.172277 | 0.073726 | 0.000634 | 0.057864 | 0.045791 | 0.089588 | 0.000877 | 0.021952 | 0.064073 | 0.079775 | 0.000742 | 0.040931 |
Eisosomes | 0.000118 | 0.000051 | 0.000033 | 0.000046 | 0.000037 | 0.000056 | 0.000058 | 0.000063 | 0.000049 | 0.000053 | 0.000044 | 0.000054 |
Endoplasmic Reticulum | 0.004989 | 0.010472 | 0.011328 | 0.008597 | 0.012436 | 0.006818 | 0.016683 | 0.015636 | 0.011359 | 0.009079 | 0.013698 | 0.011916 |
Endosome | 0.005729 | 0.007752 | 0.001451 | 0.043575 | 0.003117 | 0.006743 | 0.002173 | 0.030693 | 0.003494 | 0.007367 | 0.001771 | 0.037501 |
Golgi | 0.000552 | 0.001525 | 0.000180 | 0.190988* | 0.000587 | 0.002290 | 0.000163 | 0.031677 | 0.000582 | 0.001817 | 0.000173 | 0.115873* |
Lipid Particles | 0.015486 | 0.006624 | 0.001389 | 0.002399 | 0.005227 | 0.007398 | 0.001338 | 0.003007 | 0.006710 | 0.006919 | 0.001366 | 0.002686 |
Mitochondria | 0.002682 | 0.001974 | 0.002272 | 0.054269 | 0.002060 | 0.003321 | 0.001754 | 0.001447 | 0.002150 | 0.002488 | 0.002043 | 0.029363 |
Mitotic Spindle | 0.000724 | 0.002452 | 0.001536 | 0.108534* | 0.001541 | 0.003595 | 0.006053 | 0.009993 | 0.001423 | 0.002888 | 0.003535 | 0.062071 |
None | 0.009345 | 0.006414 | 0.002922 | 0.005945 | 0.007178 | 0.003758 | 0.004352 | 0.031213 | 0.007491 | 0.005401 | 0.003555 | 0.017859 |
Nuclear Periphery | 0.001743 | 0.003547 | 0.005197 | 0.002910 | 0.006106 | 0.004181 | 0.003616 | 0.002993 | 0.005475 | 0.003789 | 0.004497 | 0.002949 |
Nuclear Periphery Foci | 0.006402 | 0.012594 | 0.001794 | 0.003192 | 0.012080 | 0.008419 | 0.002224 | 0.005590 | 0.011259 | 0.011002 | 0.001984 | 0.004323 |
Nucleolus | 0.001201 | 0.001792 | 0.000608 | 0.000976 | 0.001870 | 0.001842 | 0.000807 | 0.000263 | 0.001773 | 0.001811 | 0.000696 | 0.000640 |
Nucleus | 0.262959* | 0.310741* | 0.868765* | 0.180019* | 0.420015* | 0.306480* | 0.788658* | 0.283288* | 0.397314* | 0.309116* | 0.833312* | 0.228710* |
Peroxisomes | 0.002655 | 0.001728 | 0.000221 | 0.002138 | 0.000642 | 0.002503 | 0.000178 | 0.001653 | 0.000933 | 0.002023 | 0.000202 | 0.001909 |
Vacuole | 0.036580 | 0.050429 | 0.039498 | 0.094385 | 0.025679 | 0.046324 | 0.084008 | 0.262157* | 0.027255 | 0.048864 | 0.059197 | 0.173490 |
Vacuole Periphery | 0.000465 | 0.000710 | 0.000208 | 0.041278 | 0.000539 | 0.000662 | 0.000229 | 0.009767 | 0.000528 | 0.000692 | 0.000217 | 0.026420 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.62 | -7.59 | -2.74 | -2.11 | 3.24 | -2.01 | -13.53 | -7.02 | -1.43 | 4.35 | -3.18 | -11.65 | -5.08 | -2.82 | 4.54 |
Bud Neck | -0.39 | -7.76 | -4.54 | -3.99 | 5.38 | -3.01 | -10.70 | -6.45 | -4.57 | 1.87 | -3.18 | -11.23 | -8.81 | -5.97 | 5.59 |
Bud Site | -1.98 | -4.50 | -3.78 | -2.94 | -2.20 | -2.33 | -8.00 | -5.16 | -2.33 | -2.37 | -3.64 | -9.07 | -6.19 | -3.88 | -3.13 |
Cell Periphery | 3.36 | -6.11 | 0.16 | -2.93 | 6.09 | -0.95 | -9.06 | -4.62 | -2.02 | 1.80 | -0.68 | -11.97 | -5.06 | -3.56 | 5.90 |
Cytoplasm | -1.31 | 29.48 | 15.81 | 20.69 | -6.78 | -3.69 | 45.29 | 14.62 | 15.81 | -4.78 | -4.80 | 60.10 | 22.74 | 25.69 | -8.15 |
Cytoplasmic Foci | 9.17 | 16.74 | 15.40 | 14.40 | -2.85 | -8.52 | 21.83 | 15.58 | 16.95 | -3.83 | -3.98 | 25.39 | 18.98 | 22.10 | -4.23 |
Eisosomes | 6.00 | 6.09 | 4.79 | -1.38 | -1.56 | -4.96 | -8.71 | -5.51 | -2.61 | 0.79 | -1.65 | -4.57 | -4.02 | -2.92 | -0.56 |
Endoplasmic Reticulum | -5.06 | -6.52 | -1.21 | 2.46 | 3.92 | 5.17 | -4.12 | 1.95 | -1.10 | 4.68 | 2.54 | -3.81 | 2.89 | 1.14 | 5.97 |
Endosome | -1.82 | 5.78 | -0.32 | 0.57 | -2.06 | -5.80 | 1.94 | -0.84 | 1.01 | -1.13 | -6.05 | 4.59 | -1.49 | 1.12 | -2.31 |
Golgi | -2.55 | 2.48 | -1.88 | -1.78 | -1.91 | -2.00 | 2.85 | -0.83 | -0.13 | -0.99 | -3.03 | 2.90 | -2.00 | -1.77 | -2.06 |
Lipid Particles | 2.94 | 4.41 | 4.18 | 5.61 | -1.24 | -2.30 | 7.24 | 2.94 | 3.97 | -3.31 | -0.41 | 7.52 | 5.51 | 6.68 | -2.90 |
Mitochondria | 0.38 | -0.10 | -0.85 | -1.17 | -0.92 | -1.01 | 0.08 | 1.90 | 1.97 | 5.51 | -0.44 | -0.66 | -0.81 | -0.66 | -0.66 |
Mitotic Spindle | -2.34 | -2.87 | -4.10 | -3.98 | -3.89 | -1.18 | -3.02 | -2.82 | -2.61 | -1.76 | -1.69 | -3.55 | -4.91 | -4.75 | -4.24 |
None | 0.90 | 2.14 | 1.01 | 0.29 | -2.95 | 3.85 | 4.56 | -0.73 | -1.67 | -1.85 | 2.10 | 5.41 | -0.16 | -1.28 | -2.72 |
Nuclear Periphery | -4.82 | -7.25 | -5.80 | -2.66 | 1.09 | 2.10 | 1.48 | 2.65 | -0.30 | 1.55 | 2.93 | -0.43 | 1.06 | -2.18 | 1.73 |
Nuclear Periphery Foci | -3.66 | 2.13 | 1.21 | 6.41 | -1.58 | 1.89 | 8.77 | 5.81 | 3.03 | -1.76 | 0.08 | 8.69 | 6.72 | 6.87 | -2.33 |
Nucleolus | -2.05 | 1.11 | 3.54 | 3.74 | 2.51 | -0.56 | 3.22 | 3.92 | 4.36 | 1.35 | -0.63 | 3.21 | 4.43 | 5.45 | 2.70 |
Nucleus | -3.35 | -31.75 | -1.51 | 0.19 | 17.39 | 10.89 | -17.97 | 3.74 | -0.49 | 13.08 | 11.13 | -34.03 | 4.29 | -0.16 | 21.78 |
Peroxisomes | 1.30 | 3.68 | 3.42 | 3.91 | -0.50 | -3.85 | 5.13 | 2.48 | 4.47 | -1.11 | -3.95 | 5.45 | 3.65 | 6.03 | -0.91 |
Vacuole | -4.07 | -5.27 | -11.14 | -10.54 | -9.64 | -6.59 | -9.97 | -12.92 | -12.07 | -8.27 | -10.27 | -11.84 | -17.13 | -15.86 | -12.80 |
Vacuole Periphery | -1.61 | 1.75 | -1.39 | -1.31 | -1.46 | -0.59 | 2.75 | -0.48 | -0.32 | -0.90 | -1.22 | 2.80 | -1.45 | -1.34 | -1.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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