Standard name
Human Ortholog
Description Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.13 0.1 0.11 0.2 0.29 0.3 – 0.21 0.2 0.17 0.23 0.2 0.21 0.19 0.19 0.21 0.18 0 0.08 0 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0.05 0.1 0.07 0.07 0 0.07 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.17 0 0.09 0.07 0.47 0.21 – 0.62 0.55 0.54 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.74 0.62 0.75 0.71 0.59 0.33 0.44 – 0.4 0.4 0.42 0.44 0.72 0.69 0.7 0.74 0.73 0.76 0.53 0.56 0.56 0.52 0.44 0.42
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.05 0.08 0.17 0.09 0.14 0.15 0.15 0.13
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.09 0.05 0.07 0 0.07 – 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.09 0.09 0.13 0.13
Vac/Vac Membrane 0.06 0.08 0 0.08 0.07 0 0.06 – 0 0 0 0.05 0 0.06 0.07 0 0 0 0.08 0.1 0.08 0.09 0.11 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud 0 1 0 4 1 3 1 – 8 2 6 2 1 0 0 0 0 0 8 7 2 3 12 11
Bud Neck 0 0 1 3 1 3 2 – 1 3 2 2 0 0 0 0 0 0 1 1 0 2 5 3
Bud Site 0 1 0 2 0 6 1 – 7 6 8 7 0 0 0 1 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Cytoplasm 23 47 20 22 39 67 72 – 55 57 45 67 37 59 69 82 68 66 13 31 7 12 14 16
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 4 0 1 7 7 8 0 0 0 0 0 2
Endosome 13 19 21 14 13 4 16 – 2 7 8 0 6 7 15 6 6 8 12 10 5 9 15 11
Golgi 0 2 0 0 0 1 0 – 0 0 0 0 0 0 1 1 0 0 1 3 0 2 2 3
Mitochondria 35 61 5 19 13 107 50 – 164 156 146 140 4 2 2 19 9 8 5 10 4 4 16 12
Nucleus 210 218 155 146 118 75 105 – 105 114 113 127 135 193 255 317 241 278 185 229 147 141 206 139
Nuclear Periphery 0 0 1 1 0 0 1 – 2 1 0 1 1 1 2 3 3 4 2 1 0 1 4 4
Nucleolus 16 13 3 2 4 3 4 – 4 6 7 9 2 4 4 27 16 29 58 34 37 39 71 41
Peroxisomes 0 1 0 0 1 0 2 – 0 0 0 0 0 1 1 4 1 0 1 0 0 0 0 0
SpindlePole 11 12 18 11 13 10 17 – 6 12 8 10 4 7 4 6 5 4 23 31 23 23 61 43
Vac/Vac Membrane 17 28 6 16 14 10 15 – 10 11 9 13 6 16 27 10 8 6 26 38 21 24 52 29
Unique Cell Count 282 350 206 206 199 228 238 263 284 270 287 188 278 363 429 331 365 349 408 261 271 472 331
Labelled Cell Count 325 403 230 240 217 289 287 365 375 352 378 200 290 381 485 366 412 349 408 261 271 472 331


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.9 5.9 5.1 6.0 4.2 5.1 – 4.2 4.2 4.1 4.5 6.0 6.2 6.2 14.9 17.3 15.7 5.6 5.2 5.8
Std Deviation (1e-4) 1.6 5.3 2.3 1.5 3.0 2.3 3.0 – 2.3 1.4 1.5 2.4 2.7 4.4 3.6 19.5 22.2 21.2 4.1 2.0 3.0
Intensity Change (Log2) – – – -0.21 0.02 -0.48 -0.21 – -0.51 -0.49 -0.52 -0.4 0.02 0.08 0.06 1.33 1.55 1.41 -0.09 -0.18 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 2.8 5.1 5.3 – 3.3 3.1 2.2 3.9 2.8 3.4 2.9 3.0 3.3 2.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -1.2 -1.3 0 -1.3 – 0 0 0 0 -2.7 -3.6 -2.9 -5.1 -4.3 -4.2
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.9 2.0 0 5.9 – 0 0 0 0 0 0 0 1.2 0.2 0
Nucleus -1.0 -3.4 -8.8 -6.6 – -7.6 -7.7 -7.3 -6.9 -0.8 -1.4 -1.3 -0.4 -0.6 0.2
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 2.7 2.1 3.2
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.3 -0.8 0 -0.6 – 0 0 0 0 -2.9 -3.1 -4.5 -4.5 -4.0 -4.6
Vacuole 2.2 1.9 0.8 1.7 – 0.5 0.6 0.3 0.9 0.2 1.5 2.2 -0.4 -0.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9385 0.2046 0.2747 0.024 0.1881 0.1096 1.2684 1.5708 1.3352 1.2858 1.2504 1.4316 3.4082 3.7905 3.798 3.1846 3.847 3.9393
Actin 0.0429 0 0.0427 0.0087 0.0139 0.0027 0.0035 0.0001 0.0045 0.0002 0.0005 0.0003 0 0.0001 0.0001 0 0.0272 0.0029
Bud 0.0005 0 0.0026 0.0004 0.0017 0.0001 0.0002 0 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001
Bud Neck 0.0018 0.0004 0.0078 0.009 0.0046 0.0024 0.0022 0.0002 0.001 0.0012 0.0006 0.0014 0.0007 0.0006 0.0003 0.0001 0.0016 0.0023
Bud Periphery 0.0007 0 0.0067 0.0009 0.0031 0.0001 0.0006 0 0.0002 0.001 0.0001 0.0001 0.0001 0.0001 0 0 0.0002 0.0001
Bud Site 0.0013 0.0002 0.0026 0.0013 0.0037 0.0005 0.0008 0 0.0014 0.0024 0.0002 0.0001 0.0003 0.0046 0.0003 0 0.0002 0.0001
Cell Periphery 0.0002 0 0.0004 0.0002 0.0005 0 0.0002 0 0 0.0002 0 0 0 0.0001 0 0 0 0
Cytoplasm 0.022 0.0092 0.0289 0.0014 0.0132 0.0019 0.0043 0.0019 0.0015 0.0009 0.0006 0.0013 0.001 0.0004 0.0338 0.0001 0.0037 0.0013
Cytoplasmic Foci 0.0105 0.0003 0.0035 0.0115 0.005 0.0019 0.0025 0.0001 0.0025 0.0001 0.0014 0.0003 0.0001 0.0001 0.0032 0 0.0036 0.0001
Eisosomes 0.0004 0 0.0006 0.0001 0.0003 0 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0082 0.0005 0.0027 0.0006 0.0023 0.0005 0.0019 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0013 0 0.0002 0.0005
Endosome 0.0303 0.0007 0.0112 0.0167 0.0211 0.0045 0.0072 0.0001 0.0007 0.0003 0.002 0.0014 0.0002 0.0001 0.0027 0 0.0019 0.0011
Golgi 0.0125 0 0.0068 0.0119 0.0068 0.0009 0.0013 0 0.0006 0.0001 0.0032 0.0003 0 0 0.0001 0 0.0021 0.0002
Lipid Particles 0.0305 0.0001 0.0054 0.0332 0.0162 0.0009 0.0037 0 0.0027 0.0003 0.0132 0.0035 0 0.0001 0.0002 0 0.0067 0
Mitochondria 0.0124 0.0002 0.0058 0.0238 0.0864 0.0005 0.0046 0.0001 0.0003 0.0039 0.0056 0.0011 0.0003 0.0002 0.0004 0 0.0009 0.0005
None 0.0203 0.0208 0.0281 0.0007 0.0056 0.0104 0.0182 0.0059 0.0144 0.0092 0.0009 0.0083 0.0003 0.0011 0.0125 0 0.0494 0.0003
Nuclear Periphery 0.0446 0.0125 0.0184 0.0038 0.0446 0.0113 0.0137 0.0067 0.003 0.0098 0.0039 0.0054 0.0018 0.0014 0.0073 0.0004 0.0059 0.0028
Nucleolus 0.0184 0.0294 0.0203 0.0143 0.0537 0.0515 0.0282 0.0222 0.0202 0.0371 0.073 0.0575 0.0245 0.0434 0.0125 0.0102 0.0475 0.0509
Nucleus 0.7053 0.885 0.7585 0.8283 0.5987 0.873 0.847 0.9444 0.9181 0.8484 0.8621 0.8846 0.9613 0.9254 0.9036 0.9444 0.7618 0.918
Peroxisomes 0.0074 0 0.0055 0.0108 0.0038 0.0003 0.0077 0 0.0016 0.0002 0.0127 0.001 0 0 0 0 0.0158 0
Punctate Nuclear 0.024 0.0401 0.0283 0.0188 0.0838 0.036 0.0493 0.018 0.0269 0.0833 0.0192 0.0323 0.0086 0.0218 0.0207 0.0448 0.0707 0.0183
Vacuole 0.0036 0.0005 0.0116 0.0027 0.0267 0.0003 0.0016 0.0001 0.0002 0.0009 0.0005 0.0004 0.0004 0.0002 0.0007 0 0.0002 0.0002
Vacuole Periphery 0.0022 0.0001 0.0018 0.001 0.0042 0.0003 0.0009 0.0001 0 0.0003 0.0003 0.0004 0.0001 0.0001 0.0002 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.3545 24.1759 35.8791 36.6882 39.7392 36.039 42.7774 47.8448 39.0407 40.3544
Translational Efficiency 1.9213 2.9752 1.868 2.0499 1.8136 2.3587 1.6479 1.5895 1.7751 1.9987

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1247 1325 547 1749 134 2305 778 791 1381 3630 1325 2540

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 593.14 691.80 892.38 733.05 709.46 774.26 855.64 853.99 604.43 744.16 870.81 770.71
Standard Deviation 70.40 105.94 105.81 105.52 78.43 99.63 114.61 109.20 79.10 109.43 112.53 120.49
Intensity Change Log 2 — 0.221982 0.589286 0.305539 — 0.126097 0.270283 0.267498 — 0.170550 0.424328 0.284944

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000269 0.000880 0.001211 0.000401 0.000513 0.000349 0.000860 0.001493 0.000293 0.000543 0.001005 0.000741
Bud Neck 0.004817 0.010500 0.014749 0.008329 0.001004 0.013533 0.008945 0.014082 0.004447 0.012426 0.011341 0.010120
Bud Site 0.001830 0.004702 0.002300 0.005775 0.000824 0.003253 0.001411 0.020463 0.001732 0.003782 0.001778 0.010349
Cell Periphery 0.000350 0.000327 0.000073 0.000341 0.000138 0.000139 0.000079 0.000182 0.000330 0.000207 0.000077 0.000292
Cytoplasm 0.118753* 0.152836* 0.003436 0.143055* 0.037251 0.079017 0.013239 0.152537* 0.110845* 0.105962* 0.009192 0.146008*
Cytoplasmic Foci 0.052562 0.034771 0.000165 0.005979 0.038698 0.010422 0.000560 0.005078 0.051216 0.019310 0.000397 0.005698
Eisosomes 0.000078 0.000054 0.000044 0.000042 0.000090 0.000032 0.000030 0.000042 0.000079 0.000040 0.000036 0.000042
Endoplasmic Reticulum 0.001769 0.004690 0.005644 0.002717 0.002596 0.001866 0.004662 0.004731 0.001850 0.002897 0.005067 0.003344
Endosome 0.001443 0.003732 0.000398 0.002130 0.001809 0.001889 0.000399 0.004005 0.001479 0.002561 0.000398 0.002714
Golgi 0.000869 0.001498 0.000153 0.001760 0.000952 0.001079 0.000124 0.001977 0.000877 0.001232 0.000136 0.001828
Lipid Particles 0.012478 0.005448 0.000348 0.000836 0.010732 0.002975 0.000414 0.001289 0.012308 0.003877 0.000387 0.000977
Mitochondria 0.008861 0.006540 0.001065 0.002090 0.007786 0.007565 0.001069 0.003324 0.008757 0.007191 0.001068 0.002474
Mitotic Spindle 0.000839 0.006248 0.003359 0.010118 0.000376 0.013303 0.003140 0.035683 0.000794 0.010728 0.003230 0.018080
None 0.051196 0.041111 0.002606 0.025828 0.016537 0.015211 0.006328 0.010411 0.047833 0.024665 0.004792 0.021027
Nuclear Periphery 0.001261 0.002270 0.000508 0.002120 0.000730 0.002575 0.000796 0.002302 0.001209 0.002463 0.000677 0.002176
Nuclear Periphery Foci 0.001516 0.003483 0.000296 0.000586 0.002022 0.001037 0.000430 0.001240 0.001565 0.001930 0.000374 0.000790
Nucleolus 0.047647 0.039641 0.029070 0.007132 0.035154 0.040565 0.020819 0.002595 0.046434 0.040227 0.024226 0.005719
Nucleus 0.683809* 0.670741* 0.931580* 0.768897* 0.835811* 0.799940* 0.934399* 0.692916* 0.698558* 0.752781* 0.933235* 0.745235*
Peroxisomes 0.007227 0.003982 0.000491 0.000765 0.003169 0.002178 0.000294 0.001279 0.006833 0.002836 0.000375 0.000925
Vacuole 0.002003 0.005442 0.002344 0.010096 0.003135 0.002332 0.001865 0.042881 0.002113 0.003467 0.002062 0.020306
Vacuole Periphery 0.000423 0.001105 0.000159 0.001002 0.000672 0.000741 0.000136 0.001491 0.000447 0.000874 0.000145 0.001154

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.58 -13.01 -5.65 4.88 10.07 0.91 -3.54 -1.63 -2.24 0.01 -5.14 -16.64 -3.77 -1.30 4.92
Bud Neck -6.28 -8.45 -6.72 -0.43 4.46 -13.26 -11.65 -8.80 -1.05 -1.24 -10.36 -11.21 -9.22 0.02 2.75
Bud Site -5.45 -2.96 -4.08 -0.72 -2.58 -4.24 -2.53 -5.98 -4.86 -5.51 -5.16 -2.28 -6.85 -4.55 -6.19
Cell Periphery 0.50 7.67 1.68 1.23 -4.72 0.51 3.29 -0.18 -1.02 -5.80 3.54 7.85 2.45 -0.72 -5.51
Cytoplasm -4.24 21.30 -0.09 4.39 -24.36 -4.99 4.45 -9.19 -6.58 -17.44 0.77 20.65 -1.79 -3.14 -29.03
Cytoplasmic Foci 4.53 16.34 14.89 11.29 -9.93 3.32 4.47 4.11 6.85 -6.23 9.94 16.90 15.52 12.90 -11.32
Eisosomes 5.74 7.28 10.26 3.97 1.79 6.16 6.18 3.32 -8.20 -8.11 12.23 12.54 9.68 -3.84 -4.53
Endoplasmic Reticulum -10.73 -13.06 -8.16 3.07 6.92 0.80 -3.36 -5.65 -12.99 -4.50 -6.50 -16.15 -13.06 -8.85 3.05
Endosome -5.15 6.72 -0.84 3.70 -3.72 0.30 2.86 -1.05 -2.01 -5.95 -4.24 8.22 -1.98 1.43 -6.29
Golgi -2.33 5.22 -0.28 0.62 -1.57 -0.28 1.79 0.21 0.51 -1.45 -1.10 5.72 -0.14 0.41 -1.98
Lipid Particles 6.40 11.83 11.42 8.95 -5.69 2.52 3.44 3.08 4.23 -6.91 8.38 12.33 11.67 9.67 -8.66
Mitochondria 1.69 5.82 5.27 6.80 -2.32 -0.07 1.14 0.94 6.00 -3.22 1.16 5.81 5.18 8.61 -3.61
Mitotic Spindle -5.19 -2.52 -6.28 -2.49 -1.81 -7.38 -2.89 -6.25 -2.74 -4.65 -8.42 -3.60 -8.75 -2.50 -4.67
None 2.55 15.13 8.65 6.20 -9.38 0.51 3.44 3.08 7.36 -1.11 7.38 14.69 10.16 5.20 -8.31
Nuclear Periphery -3.14 4.07 -3.00 -0.60 -4.26 -5.51 -1.41 -10.44 -3.70 -11.38 -5.55 2.27 -5.58 -1.42 -6.66
Nuclear Periphery Foci -4.59 5.78 3.98 6.38 -2.21 0.95 1.63 0.73 -0.80 -3.96 -1.89 5.97 3.40 5.23 -3.95
Nucleolus 2.54 3.94 15.27 13.80 8.19 -1.05 2.31 6.36 20.33 9.48 2.16 6.85 16.98 22.72 11.41
Nucleus 0.56 -23.67 -10.94 -11.56 15.02 1.18 -5.17 5.60 8.62 20.55 -6.29 -26.56 -7.26 -1.63 25.16
Peroxisomes 3.26 7.26 7.11 5.78 -0.01 1.03 4.20 3.54 4.92 -1.76 4.60 7.68 7.38 7.56 -1.13
Vacuole -4.05 -1.36 -8.85 -7.14 -7.13 1.16 1.27 -13.57 -13.82 -13.83 -1.85 -0.79 -15.49 -15.36 -15.42
Vacuole Periphery -6.22 4.12 -1.07 1.39 -1.95 -0.19 1.64 -0.12 0.08 -2.56 -3.48 4.79 -1.31 0.71 -2.80
Standard name
Human Ortholog
Description Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
Localization
Cell Percentages nucleus (71%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yra2

Yra2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yra2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available