Standard name
Human Ortholog
Description Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.99 0.98 0.95 0.9 0.84 0.87 0.81 0.85 0.78 0.99 1.0 0.99 0.97 0.98 0.98 0.97 0.97 0.93 0.96 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.07 0.05 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.11 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 2 4 4 2 9 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 3 0 0 2 3 3 1 1 0 0 2 0 0 0 0 0 0 2 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 2 3 4 9 8 15 8 2 16 1 4 3 3 2 2 0 0 0 0 0 0
Cytoplasm 120 160 106 198 390 272 343 307 261 284 204 291 397 519 553 246 139 219 117 146 109 239 380 435
Endoplasmic Reticulum 0 2 0 0 6 6 24 26 15 22 3 27 4 0 3 7 6 8 0 0 1 0 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3 8 8 17 10 0 0 0 2 1 2 0 0 1 0 0 1
Nucleus 1 1 1 1 2 2 6 9 8 2 2 5 1 1 0 2 0 2 0 1 1 2 6 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 1 5 8 23 9 37 16 42 1 1 0 4 1 0 0 0 0 0 3 4
Unique Cell Count 121 162 109 199 397 285 383 366 301 350 239 373 403 519 556 254 142 223 121 152 117 249 402 458
Labelled Cell Count 121 163 110 202 406 289 392 380 323 368 247 401 405 525 561 265 151 235 121 152 117 249 402 458


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.6 58.8 35.8 41.2 32.8 25.4 21.8 19.4 17.6 15.5 15.1 15.5 45.6 46.0 44.4 52.0 55.8 52.2 49.1 48.1 50.3
Std Deviation (1e-4) 9.7 13.0 9.9 9.4 7.4 5.9 5.4 4.7 4.5 3.8 3.9 3.4 11.4 9.5 8.8 19.1 16.2 16.9 13.5 12.6 12.8
Intensity Change (Log2) – – – 0.2 -0.13 -0.49 -0.72 -0.89 -1.03 -1.21 -1.25 -1.21 0.35 0.36 0.31 0.54 0.64 0.54 0.45 0.43 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 0.7 -0.8 -2.5 -3.6 -3.1 -4.1 -3.3 -4.6 0.9 3.8 2.2 -0.2 0.3 0.6
Endoplasmic Reticulum 0 0 0 2.7 2.9 0 2.7 0 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.2 0 3.2 2.3 3.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.5696 43.8819 42.32 37.2242 34.9684 40.4433 44.5849 57.8814 50.456 48.487 43.8486 52.2524 51.8888 56.6271 55.4229 51.6244 54.2262 53.5632
Actin 0.0009 0.0001 0.0001 0.0004 0.0005 0.0001 0.0088 0 0.0029 0.0184 0.0012 0.0001 0.0001 0.0001 0 0 0 0.0001
Bud 0.0002 0.0001 0 0.0012 0.0002 0 0.0009 0 0.0021 0 0.0009 0.0001 0 0 0 0 0 0
Bud Neck 0.0002 0.0001 0.0002 0.0002 0.0004 0.0005 0.0004 0.0001 0.0007 0.0001 0.0005 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003
Bud Periphery 0.0001 0 0 0.0005 0.0004 0 0.0005 0 0.0017 0 0.0004 0 0 0 0 0 0 0
Bud Site 0.0001 0 0 0.0001 0.0009 0 0.0012 0 0.0047 0 0.0005 0 0 0 0 0 0 0
Cell Periphery 0.0001 0 0 0.0001 0.001 0 0.0002 0 0.0003 0 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.941 0.9841 0.9823 0.9502 0.8701 0.9666 0.8568 0.9952 0.9269 0.9053 0.8234 0.9844 0.9629 0.9953 0.9872 0.9854 0.9918 0.982
Cytoplasmic Foci 0.0023 0.0003 0.0002 0.0086 0.0141 0.0002 0.0206 0.0001 0.0088 0.0028 0.026 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002
Eisosomes 0 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.001 0.0004 0.0004 0.0008 0.0023 0.0007 0.0014 0.0004 0.0028 0.0001 0.0018 0.0005 0.0005 0.0001 0.0002 0.0002 0.0001 0.0003
Endosome 0.001 0 0.0001 0.0027 0.007 0.0001 0.0286 0 0.007 0.0001 0.0094 0.0002 0.0001 0 0 0 0 0.0001
Golgi 0.0003 0 0 0.0003 0.0014 0 0.0085 0 0.003 0.0005 0.0013 0 0 0 0 0 0 0
Lipid Particles 0.0019 0 0 0.0005 0.0224 0 0.01 0 0.0021 0.0003 0.0027 0 0 0 0 0 0 0
Mitochondria 0.0005 0 0 0.0046 0.0149 0 0.0132 0 0.0126 0.0001 0.0559 0.0001 0.0001 0 0 0 0 0
None 0.001 0.0002 0.0002 0.0034 0.0095 0.0001 0.0023 0.0001 0.0037 0.0001 0.0045 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.007 0.002 0.0027 0.0042 0.0094 0.0051 0.0034 0.0005 0.0018 0.0008 0.0056 0.0022 0.0057 0.0007 0.0014 0.0028 0.0008 0.0019
Nucleolus 0.0002 0 0 0.0006 0.0049 0 0.0016 0 0.001 0 0.0044 0 0 0 0 0 0 0
Nucleus 0.0363 0.0121 0.0136 0.0144 0.0245 0.026 0.0159 0.0036 0.0088 0.01 0.0291 0.0107 0.0289 0.0033 0.0107 0.011 0.0067 0.0147
Peroxisomes 0.0031 0 0 0.0005 0.0012 0 0.0141 0 0.0015 0.061 0.0013 0 0 0 0 0 0 0
Punctate Nuclear 0.0009 0.0001 0.0001 0.0007 0.0038 0.0001 0.0017 0 0.0006 0.0001 0.0093 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0016 0.0002 0.0002 0.0034 0.0072 0.0004 0.0072 0.0001 0.0048 0.0001 0.0115 0.0006 0.0008 0.0001 0.0001 0.0002 0.0001 0.0002
Vacuole Periphery 0.0004 0 0 0.0028 0.0036 0.0001 0.0024 0 0.0021 0 0.0099 0.0002 0.0003 0 0 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 96.329 93.9406 58.09 72.6962 77.9646 49.013 69.3905 55.2035 51.8684 86.4728
Translational Efficiency 2.0814 1.888 2.4421 1.5269 1.9364 3.2465 2.1136 1.9841 1.7655 1.9049

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
412 350 1874 127 1961 944 284 1359 2373 1294 2158 1486

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2928.69 4101.39 4156.36 4946.25 2885.27 3573.68 4161.95 3846.91 2892.81 3716.41 4157.10 3940.86
Standard Deviation 602.99 732.61 802.58 947.70 604.15 664.46 750.82 828.07 604.17 722.64 795.96 893.49
Intensity Change Log 2 — 0.485857 0.505065 0.756080 — 0.308704 0.528553 0.414994 — 0.400659 0.516769 0.596861

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000201 0.000091 0.000064 0.000302 0.000072 0.000075 0.000251 0.000797 0.000095 0.000079 0.000088 0.000755
Bud Neck 0.000879 0.001096 0.000632 0.003159 0.000293 0.000557 0.003090 0.003272 0.000395 0.000703 0.000956 0.003263
Bud Site 0.001954 0.000916 0.000558 0.004074 0.000568 0.001641 0.001648 0.008224 0.000808 0.001445 0.000702 0.007869
Cell Periphery 0.001333 0.000644 0.000303 0.004366 0.000237 0.000215 0.001520 0.000271 0.000428 0.000331 0.000463 0.000621
Cytoplasm 0.805770* 0.718699* 0.657507* 0.642693* 0.793177* 0.735656* 0.499290* 0.683134* 0.795364* 0.731069* 0.636685* 0.679678*
Cytoplasmic Foci 0.013113 0.011126 0.004267 0.028861 0.002932 0.004111 0.026095 0.006269 0.004700 0.006008 0.007140 0.008200
Eisosomes 0.000001 0.000001 0.000001 0.000007 0.000000 0.000001 0.000004 0.000004 0.000001 0.000001 0.000001 0.000004
Endoplasmic Reticulum 0.000104 0.000030 0.000019 0.000054 0.000048 0.000039 0.000055 0.000477 0.000057 0.000037 0.000024 0.000441
Endosome 0.000170 0.000092 0.000029 0.000112 0.000054 0.000886 0.000124 0.003256 0.000074 0.000671 0.000042 0.002987
Golgi 0.002046 0.001448 0.000227 0.001917 0.000596 0.000552 0.000755 0.004600 0.000848 0.000794 0.000297 0.004371
Lipid Particles 0.002377 0.000798 0.000230 0.003997 0.000358 0.000230 0.001804 0.000690 0.000709 0.000384 0.000437 0.000973
Mitochondria 0.000085 0.000101 0.000191 0.000904 0.000128 0.000090 0.000229 0.001289 0.000121 0.000093 0.000196 0.001256
Mitotic Spindle 0.000779 0.001062 0.000594 0.000378 0.000423 0.000400 0.002792 0.015319 0.000485 0.000579 0.000883 0.014042
None 0.011123 0.018477 0.011769 0.014464 0.014596 0.019815 0.010932 0.008847 0.013993 0.019453 0.011659 0.009327
Nuclear Periphery 0.000118 0.000161 0.000186 0.001035 0.000068 0.000075 0.000660 0.000307 0.000076 0.000098 0.000248 0.000370
Nuclear Periphery Foci 0.004248 0.002293 0.000402 0.003532 0.001135 0.000850 0.002246 0.001264 0.001676 0.001240 0.000644 0.001458
Nucleolus 0.000069 0.000108 0.000079 0.000098 0.000065 0.000089 0.000132 0.000272 0.000065 0.000094 0.000086 0.000258
Nucleus 0.146829* 0.233745* 0.319081* 0.239876* 0.182195* 0.230948* 0.429133* 0.243193* 0.176054* 0.231704* 0.333564* 0.242910*
Peroxisomes 0.000011 0.000006 0.000004 0.000013 0.000004 0.000011 0.000014 0.000135 0.000006 0.000009 0.000006 0.000125
Vacuole 0.008561 0.005931 0.003786 0.048861 0.002812 0.003563 0.019090 0.016272 0.003811 0.004204 0.005800 0.019057
Vacuole Periphery 0.000229 0.003174 0.000070 0.001297 0.000237 0.000197 0.000135 0.002105 0.000236 0.001002 0.000079 0.002036

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.11 4.27 -2.23 -4.72 -5.50 -0.68 -8.99 -2.15 -2.07 1.15 0.72 -2.57 -2.12 -2.21 -1.78
Bud Neck -0.62 -1.27 -6.41 -4.97 -5.78 -2.66 -10.74 -8.10 -6.76 4.95 -2.80 -9.27 -8.70 -6.90 -3.14
Bud Site 2.13 4.81 -5.43 -6.69 -8.48 -2.16 -9.66 -6.00 -4.20 -1.83 -1.91 -4.05 -6.15 -4.81 -5.49
Cell Periphery 1.52 2.97 -3.35 -4.32 -5.02 0.15 -10.81 -3.21 -3.24 10.28 0.42 -4.73 -2.59 -3.12 1.71
Cytoplasm 8.28 18.93 12.21 6.97 4.32 10.55 27.78 22.59 10.18 -14.98 13.44 33.71 25.21 10.99 -4.87
Cytoplasmic Foci -1.18 4.40 -5.89 -5.26 -7.11 -3.18 -10.53 -10.36 -7.60 8.65 -4.17 -9.35 -10.08 -5.99 -0.97
Eisosomes 0.43 1.61 -5.76 -5.84 -6.06 -1.61 -9.34 -7.46 -6.84 6.42 -1.84 -8.43 -8.72 -7.98 -3.43
Endoplasmic Reticulum 3.26 3.61 2.98 -0.73 -1.93 1.02 -4.10 -0.92 -1.01 -0.44 2.71 3.76 -0.80 -1.02 -1.04
Endosome 2.17 3.73 2.06 -0.60 -3.11 -1.35 -6.39 -2.57 -0.28 -1.99 -1.31 -0.34 -2.50 -0.57 -2.48
Golgi 1.43 5.10 2.68 0.65 -2.01 0.62 -3.27 -0.86 -1.00 -0.24 0.84 5.66 -0.43 -0.69 -1.30
Lipid Particles 2.43 3.37 -3.39 -5.65 -6.33 1.22 -8.13 -7.60 -9.53 5.74 2.04 -1.66 -6.03 -9.38 -5.37
Mitochondria -1.20 -11.64 -1.02 -0.85 -0.10 1.98 -5.47 -1.29 -1.46 -0.44 1.41 -7.41 -1.39 -1.51 -0.69
Mitotic Spindle -0.96 -0.51 0.22 1.04 0.74 0.24 -1.74 -4.34 -4.37 -2.56 -0.93 -2.81 -4.31 -4.02 -3.78
None -2.17 -0.44 2.11 3.74 3.22 -2.75 6.54 8.83 10.14 1.81 -3.10 4.15 9.02 10.55 7.04
Nuclear Periphery -3.15 -8.95 -4.44 -3.85 -3.02 -3.06 -12.59 -13.35 -12.31 7.68 -5.20 -17.78 -13.75 -11.92 -1.20
Nuclear Periphery Foci 3.11 6.17 1.56 -1.94 -6.56 1.09 -7.41 -1.46 -2.48 6.15 2.07 4.99 0.70 -1.36 -4.33
Nucleolus -1.13 -4.77 -1.47 0.76 2.36 -2.87 -10.04 -5.63 -4.54 -1.72 -2.75 -8.85 -5.63 -4.14 -3.67
Nucleus -9.13 -23.95 -4.21 1.84 7.74 -9.26 -18.49 -9.71 0.38 13.05 -12.10 -32.17 -11.15 1.05 17.74
Peroxisomes 1.81 3.24 -2.71 -3.55 -4.07 -2.25 -8.64 -4.19 -3.56 -1.44 -2.09 -6.20 -4.21 -3.73 -3.64
Vacuole 0.73 1.25 -9.02 -9.14 -9.22 -3.47 -10.88 -19.98 -18.49 -1.78 -3.33 -10.78 -21.31 -20.03 -16.73
Vacuole Periphery -1.00 3.42 -0.81 0.92 -1.44 0.69 0.61 -1.06 -1.25 -1.19 -0.92 2.59 -1.12 0.34 -1.56
Standard name
Human Ortholog
Description Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ura1

Ura1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available