Standard name
Human Ortholog
Description Member of an oxysterol-binding protein family; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.12 0.28 0.31 0.32 0.36 0.21 0.25 0.16 0.12 0.18 0.2 0 0.05 0 0.18 0.17 0.19 0.17 0.16 0.18 0.14 0.09 0.11
Bud 0.14 0.06 0.1 0.1 0.18 0.12 0.12 0.08 0.1 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.06 0.08 0.07 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.47 0.6 0.6 0.63 0.37 0.41 0.33 0.28 0.31 0.29 0.31 0.3 0.52 0.71 0.63 0.78 0.82 0.83 0.49 0.59 0.66 0.42 0.57 0.63
Cytoplasm 0.54 0.44 0.38 0.27 0.26 0.15 0.18 0.14 0.18 0.18 0.11 0.13 0.81 0.72 0.72 0.23 0.18 0.17 0.13 0.07 0 0.23 0.17 0.12
Endoplasmic Reticulum 0 0.09 0.06 0.06 0.1 0.12 0.07 0.16 0.08 0.06 0.07 0.12 0 0 0 0 0 0 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.27 0.24 0.56 0.61 0.61 0.65 0.71 0.78 0.74 0 0 0 0 0.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 14 57 57 43 80 68 94 48 53 46 72 6 11 3 29 26 28 27 21 43 23 17 31
Bud 20 7 19 19 24 26 39 29 30 31 19 16 6 1 0 5 6 5 0 0 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16 13 14 12 14 19
Bud Site 1 0 1 4 0 0 3 1 3 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 66 69 120 114 50 91 107 103 93 128 82 110 109 151 155 124 125 123 79 79 164 70 108 186
Cytoplasm 75 51 75 50 35 34 58 51 55 81 28 47 170 155 178 37 27 26 21 9 8 38 32 34
Endoplasmic Reticulum 3 10 11 11 14 28 22 61 24 25 17 45 4 9 9 6 2 6 10 3 5 5 5 9
Endosome 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 0 0 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 2 1 0 0 1 0 1 0
Mitochondria 4 3 11 49 33 125 198 226 195 318 203 267 0 0 1 6 15 3 2 1 3 7 2 1
Nucleus 0 0 0 0 0 0 0 0 2 1 0 0 1 1 0 3 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 4 1 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 7 7 5 10 10 2 8 3 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 4 1 2 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 5 6 0 7 1 17 16 3 5 0 2 3 0 1 0 1 0 0 3 3 3
Unique Cell Count 139 115 200 182 135 224 327 371 299 447 261 363 209 214 247 160 152 149 163 135 247 167 190 295
Labelled Cell Count 189 154 296 319 217 390 514 581 473 664 402 567 296 332 350 213 205 193 163 135 247 167 190 295


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 8.6 7.0 6.6 7.3 6.3 6.2 6.0 5.8 5.7 5.4 5.4 9.2 9.2 9.0 12.4 12.3 13.0 8.1 9.5 10.0
Std Deviation (1e-4) 0.7 1.1 1.2 1.3 2.0 1.1 1.5 1.0 1.1 1.0 1.0 1.0 1.3 1.2 1.4 2.2 2.1 2.2 1.3 1.6 1.5
Intensity Change (Log2) – – – -0.09 0.04 -0.17 -0.18 -0.22 -0.27 -0.3 -0.39 -0.39 0.39 0.39 0.35 0.82 0.81 0.88 0.21 0.44 0.5

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 0.7 1.6 -2.0 -0.8 -3.3 -5.2 -2.8 -2.3 -7.2 -6.4 -8.4 -2.3 -2.5 -2.1
Bud 0.3 2.2 0.7 0.9 -0.7 0.2 -1.1 -0.9 -2.4 -2.8 -4.3 -5.0 -2.4 -2.0 -2.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 -4.1 -4.0 -6.1 -7.5 -6.4 -7.6 -6.1 -6.9 -1.6 2.3 0.6 3.5 4.5 4.5
Cytoplasm -2.1 -2.2 -5.3 -5.1 -6.5 -4.8 -5.3 -6.8 -6.8 9.0 7.1 7.3 -2.9 -4.0 -4.1
Endoplasmic Reticulum 0.2 1.7 2.5 0.6 3.8 1.1 0 0.5 2.6 -1.9 -0.6 -0.9 -0.8 -2.1 -0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.7 5.0 11.1 12.5 12.8 13.3 15.4 15.4 15.5 -3.4 -3.5 -3.3 -0.8 1.6 -1.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 3.0 2.3 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0995 5.5439 4.5785 4.396 3.9358 4.7201 4.4634 5.2898 5.6366 5.3752 4.6014 5.3081 3.5969 4.9292 4.0918 4.0197 4.2736 4.2176
Actin 0.021 0.036 0.0371 0.0444 0.028 0.0183 0.1026 0.0414 0.047 0.0554 0.0766 0.0412 0.0406 0.0136 0.0256 0.0157 0.0639 0.0359
Bud 0.0005 0.0004 0.0007 0.0011 0.0013 0.0004 0.001 0.0013 0.0018 0.0043 0.0005 0.0005 0.0006 0.0004 0.0005 0.0008 0.0007 0.0009
Bud Neck 0.005 0.0107 0.0268 0.07 0.0081 0.0251 0.0094 0.0056 0.019 0.0245 0.0097 0.0238 0.0086 0.0056 0.0424 0.0497 0.0519 0.0453
Bud Periphery 0.0024 0.0016 0.0036 0.007 0.0088 0.0036 0.0026 0.0052 0.0045 0.0176 0.0021 0.0018 0.0036 0.0014 0.0031 0.0069 0.0035 0.0058
Bud Site 0.0153 0.0834 0.0463 0.0509 0.0067 0.0019 0.0118 0.051 0.0386 0.0275 0.0029 0.0015 0.0191 0.1095 0.0375 0.0105 0.0064 0.0029
Cell Periphery 0.1764 0.1194 0.161 0.1828 0.0948 0.1587 0.1016 0.087 0.075 0.1278 0.0952 0.0689 0.2216 0.1466 0.1896 0.2146 0.08 0.1425
Cytoplasm 0.736 0.7246 0.6775 0.6301 0.7612 0.7448 0.6131 0.7697 0.7453 0.6781 0.5551 0.7908 0.6497 0.7018 0.6785 0.6731 0.7746 0.7489
Cytoplasmic Foci 0.0026 0.0031 0.0028 0.0011 0.0141 0.0018 0.0268 0.0045 0.006 0.0112 0.0239 0.0062 0.0071 0.0011 0.0016 0.001 0.0045 0.0016
Eisosomes 0.0029 0.0017 0.0019 0.0012 0.001 0.002 0.0062 0.0008 0.0007 0.0007 0.0011 0.0007 0.0047 0.0011 0.0029 0.002 0.0016 0.0023
Endoplasmic Reticulum 0.0089 0.0051 0.0083 0.0052 0.0146 0.0061 0.0235 0.0116 0.0156 0.0134 0.0096 0.0158 0.0075 0.0064 0.0074 0.0213 0.005 0.0066
Endosome 0.0007 0.0003 0.0004 0.0002 0.0117 0.0001 0.0164 0.0009 0.0107 0.0043 0.065 0.0124 0.0019 0.0002 0.0003 0.0001 0.0004 0.0002
Golgi 0.0012 0.0021 0.0015 0.0006 0.0035 0.0008 0.0156 0.0026 0.0044 0.0052 0.0593 0.011 0.0027 0.0008 0.0012 0.001 0.0031 0.0015
Lipid Particles 0.0025 0.001 0.0011 0.0004 0.0122 0.0006 0.0124 0.0038 0.001 0.0019 0.0501 0.0029 0.0062 0.0005 0.001 0.001 0.001 0.0006
Mitochondria 0.0005 0.0001 0.0001 0.0001 0.0012 0.0001 0.0036 0.0003 0.0032 0.0014 0.0248 0.0049 0.0004 0 0 0 0.0001 0.0002
None 0.0199 0.0089 0.0289 0.0031 0.0071 0.0346 0.0308 0.0118 0.0205 0.0115 0.0045 0.0016 0.0182 0.0097 0.007 0.0007 0.0024 0.0031
Nuclear Periphery 0.0006 0.0003 0.0003 0.0002 0.0017 0.0001 0.003 0.0005 0.0014 0.0017 0.0011 0.0072 0.0004 0.0003 0.0003 0.0004 0.0002 0.0002
Nucleolus 0.0003 0.0001 0.0001 0.0001 0.0011 0 0.0003 0.0001 0.0001 0.0003 0.0003 0.0003 0.0001 0 0 0 0 0.0001
Nucleus 0.0005 0.0003 0.0003 0.0005 0.0017 0.0002 0.0024 0.0004 0.0007 0.0016 0.0004 0.0013 0.0003 0.0003 0.0003 0.0003 0.0002 0.0005
Peroxisomes 0.0003 0.0001 0.0002 0.0001 0.0066 0 0.0089 0.0002 0.001 0.0046 0.0117 0.0013 0.0048 0 0.0001 0 0.0002 0.0001
Punctate Nuclear 0.0004 0.0001 0.0001 0.0001 0.0108 0.0001 0.0044 0.0002 0.0002 0.0056 0.0014 0.0045 0.0007 0.0001 0.0001 0.0001 0.0001 0.0002
Vacuole 0.002 0.0006 0.0009 0.0009 0.0037 0.0006 0.0029 0.0009 0.0027 0.0012 0.0031 0.0009 0.0011 0.0004 0.0006 0.0008 0.0003 0.0005
Vacuole Periphery 0.0001 0 0 0 0.0002 0 0.0005 0 0.0007 0.0001 0.0018 0.0006 0.0001 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.9843 14.6455 17.9316 26.6778 24.9587 25.3798 22.5823 25.4859 29.0849 32.1838
Translational Efficiency 0.9628 1.0763 0.7677 0.8999 0.6334 0.9539 0.7883 0.7564 0.8388 0.6352

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
299 100 863 1383 1646 1734 147 894 1945 1834 1010 2277

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 852.78 1034.66 1112.21 1150.41 870.71 925.17 1243.23 1128.95 867.95 931.14 1131.28 1141.98
Standard Deviation 135.88 146.44 153.22 160.00 115.01 110.92 144.30 172.70 118.63 115.84 158.83 165.43
Intensity Change Log 2 — 0.278911 0.383184 0.431903 — 0.087526 0.513829 0.374717 — 0.185396 0.450664 0.403296

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.036458 0.091571 0.081541 0.154336 0.045894 0.067087 0.106173 0.113095 0.044443 0.068422 0.085126 0.138144
Bud Neck 0.022212 0.075605 0.081173 0.037013 0.062804 0.084770 0.083110 0.029361 0.056564 0.084271 0.081455 0.034008
Bud Site 0.135649 0.174394 0.158300 0.170304 0.100370 0.099741 0.189433 0.137489 0.105793 0.103812 0.162831 0.157420
Cell Periphery 0.582992 0.200981 0.448679 0.375698 0.261695 0.100171 0.365570 0.242432 0.311087 0.105668 0.436583 0.323375
Cytoplasm 0.108077 0.252383 0.089231 0.128759 0.359979 0.434702 0.060950 0.214546 0.321255 0.424761 0.085114 0.162441
Cytoplasmic Foci 0.012170 0.033236 0.002155 0.003135 0.021982 0.029833 0.005273 0.044060 0.020474 0.030018 0.002608 0.019203
Eisosomes 0.007290 0.044843 0.039863 0.058468 0.064567 0.090007 0.062541 0.043738 0.055762 0.087544 0.043164 0.052684
Endoplasmic Reticulum 0.022917 0.035095 0.059467 0.013578 0.015400 0.005960 0.053028 0.009148 0.016556 0.007549 0.058530 0.011839
Endosome 0.003114 0.005678 0.001823 0.001895 0.003647 0.003547 0.001451 0.010364 0.003565 0.003664 0.001769 0.005220
Golgi 0.005331 0.011516 0.002366 0.002249 0.011373 0.011627 0.002462 0.008004 0.010444 0.011621 0.002380 0.004509
Lipid Particles 0.004511 0.007198 0.006967 0.006010 0.005929 0.006791 0.004992 0.009700 0.005711 0.006813 0.006679 0.007459
Mitochondria 0.029244 0.002631 0.001002 0.001654 0.006182 0.005081 0.000125 0.004714 0.009727 0.004948 0.000874 0.002855
Mitotic Spindle 0.000267 0.000201 0.000372 0.000838 0.000570 0.002037 0.000199 0.001943 0.000524 0.001937 0.000347 0.001272
None 0.000372 0.000074 0.000130 0.000150 0.000390 0.000787 0.000031 0.000152 0.000388 0.000748 0.000116 0.000150
Nuclear Periphery 0.000150 0.000065 0.000217 0.000193 0.000095 0.000060 0.000116 0.000149 0.000103 0.000061 0.000202 0.000175
Nuclear Periphery Foci 0.000696 0.001531 0.001264 0.000961 0.000462 0.000421 0.001853 0.001275 0.000498 0.000482 0.001349 0.001084
Nucleolus 0.001877 0.000048 0.000027 0.000017 0.000062 0.000107 0.000011 0.000065 0.000341 0.000104 0.000025 0.000036
Nucleus 0.012196 0.010693 0.004412 0.007206 0.007734 0.018367 0.007586 0.010921 0.008420 0.017949 0.004874 0.008665
Peroxisomes 0.009308 0.010178 0.004222 0.001908 0.021347 0.014551 0.000753 0.005099 0.019496 0.014313 0.003717 0.003160
Vacuole 0.003601 0.041566 0.016645 0.034459 0.008917 0.023963 0.054287 0.109943 0.008100 0.024923 0.022124 0.064095
Vacuole Periphery 0.001567 0.000512 0.000146 0.001172 0.000599 0.000387 0.000057 0.003804 0.000747 0.000394 0.000133 0.002205

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.72 -6.80 -16.05 -4.24 -10.52 -6.06 -4.94 -10.39 -6.79 -0.35 -7.15 -8.88 -20.66 -14.29 -9.06
Bud Neck -4.16 -11.08 -3.96 3.08 9.64 -5.59 -2.08 10.72 16.18 5.76 -7.57 -5.55 8.72 16.10 11.73
Bud Site -1.75 -1.83 -2.93 0.17 -1.54 0.15 -5.23 -5.13 -5.33 3.06 0.44 -8.47 -9.85 -10.45 0.87
Cell Periphery 11.11 5.90 9.41 -6.05 6.17 21.60 -4.46 2.27 -12.55 5.35 26.08 -11.41 -0.83 -28.49 10.98
Cytoplasm -6.33 1.82 -2.09 5.80 -6.54 -9.21 31.97 14.94 23.69 -15.05 -13.28 34.04 22.67 39.30 -13.96
Cytoplasmic Foci -2.67 5.04 4.45 3.91 -2.25 -3.58 8.11 -6.40 -4.20 -11.21 -4.68 12.96 0.38 4.98 -12.09
Eisosomes -4.44 -15.46 -20.85 -1.55 -6.22 -6.99 0.30 6.44 12.21 2.73 -9.39 4.63 1.45 10.90 -3.44
Endoplasmic Reticulum -1.16 -6.78 2.49 2.15 10.84 7.78 -4.38 4.54 -2.75 5.12 6.83 -10.94 3.64 -3.77 12.36
Endosome -1.20 2.04 1.65 1.79 -0.13 0.17 4.83 -5.13 -5.28 -7.04 -0.24 4.43 -2.46 -2.33 -5.69
Golgi -1.93 1.97 1.94 3.23 0.22 -0.22 9.74 2.11 2.28 -3.49 -1.08 10.49 5.98 6.96 -2.90
Lipid Particles -0.96 -0.94 -0.57 0.79 0.85 -1.60 0.73 -3.42 -2.66 -3.06 -1.77 -1.28 -2.36 -1.08 -1.12
Mitochondria 5.05 5.52 5.35 0.70 -1.00 1.21 11.03 1.16 0.35 -3.47 4.11 9.44 6.16 2.29 -2.99
Mitotic Spindle 1.10 -2.46 -1.69 -1.88 -1.39 -1.82 1.13 -1.88 0.18 -2.66 -1.88 0.64 -1.76 0.91 -2.81
None 1.79 1.47 1.34 -2.07 -0.54 -2.18 7.12 4.13 3.67 -3.57 -2.07 5.32 4.42 3.67 -1.23
Nuclear Periphery 1.43 -1.12 -0.58 -2.89 0.55 5.86 -1.57 -2.68 -4.37 -1.35 4.06 -7.25 -2.49 -4.15 0.94
Nuclear Periphery Foci -1.72 -1.63 -0.81 1.43 1.48 1.12 -5.39 -7.43 -7.68 1.91 0.21 -6.01 -5.94 -6.56 1.48
Nucleolus 1.29 1.31 1.32 2.39 4.64 -6.54 15.27 -0.10 2.53 -3.37 1.09 1.46 1.41 8.04 -1.70
Nucleus 0.35 1.56 1.00 1.50 -4.69 -12.13 0.02 -4.67 7.41 -2.27 -8.17 3.71 -0.37 10.93 -7.13
Peroxisomes -0.37 3.54 5.40 4.39 4.73 4.30 16.11 11.78 8.99 -7.63 3.65 13.48 14.53 12.45 1.25
Vacuole -4.73 -5.75 -11.07 0.76 -8.69 -12.06 -5.85 -17.29 -14.77 -6.34 -13.17 -9.20 -20.39 -13.96 -14.30
Vacuole Periphery 2.82 4.73 0.80 -1.43 -2.55 2.19 6.78 -2.75 -2.94 -3.24 3.61 7.47 -2.76 -3.49 -4.03
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Member of an oxysterol-binding protein family; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (31%), cytoplasm (8%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Osh6

Osh6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Osh6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available