Standard name | |
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Human Ortholog | |
Description | Member of an oxysterol-binding protein family; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 20 | 14 | 57 | 57 | 43 | 80 | 68 | 94 | 48 | 53 | 46 | 72 | 6 | 11 | 3 | 29 | 26 | 28 | 27 | 21 | 43 | 23 | 17 | 31 |
Bud | 20 | 7 | 19 | 19 | 24 | 26 | 39 | 29 | 30 | 31 | 19 | 16 | 6 | 1 | 0 | 5 | 6 | 5 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 13 | 14 | 12 | 14 | 19 |
Bud Site | 1 | 0 | 1 | 4 | 0 | 0 | 3 | 1 | 3 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 66 | 69 | 120 | 114 | 50 | 91 | 107 | 103 | 93 | 128 | 82 | 110 | 109 | 151 | 155 | 124 | 125 | 123 | 79 | 79 | 164 | 70 | 108 | 186 |
Cytoplasm | 75 | 51 | 75 | 50 | 35 | 34 | 58 | 51 | 55 | 81 | 28 | 47 | 170 | 155 | 178 | 37 | 27 | 26 | 21 | 9 | 8 | 38 | 32 | 34 |
Endoplasmic Reticulum | 3 | 10 | 11 | 11 | 14 | 28 | 22 | 61 | 24 | 25 | 17 | 45 | 4 | 9 | 9 | 6 | 2 | 6 | 10 | 3 | 5 | 5 | 5 | 9 |
Endosome | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 4 | 3 | 11 | 49 | 33 | 125 | 198 | 226 | 195 | 318 | 203 | 267 | 0 | 0 | 1 | 6 | 15 | 3 | 2 | 1 | 3 | 7 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 7 | 7 | 5 | 10 | 10 | 2 | 8 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 5 | 6 | 0 | 7 | 1 | 17 | 16 | 3 | 5 | 0 | 2 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 3 | 3 |
Unique Cell Count | 139 | 115 | 200 | 182 | 135 | 224 | 327 | 371 | 299 | 447 | 261 | 363 | 209 | 214 | 247 | 160 | 152 | 149 | 163 | 135 | 247 | 167 | 190 | 295 |
Labelled Cell Count | 189 | 154 | 296 | 319 | 217 | 390 | 514 | 581 | 473 | 664 | 402 | 567 | 296 | 332 | 350 | 213 | 205 | 193 | 163 | 135 | 247 | 167 | 190 | 295 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 8.6 | 7.0 | 6.6 | 7.3 | 6.3 | 6.2 | 6.0 | 5.8 | 5.7 | 5.4 | 5.4 | 9.2 | 9.2 | 9.0 | 12.4 | 12.3 | 13.0 | 8.1 | 9.5 | 10.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.2 | 1.3 | 2.0 | 1.1 | 1.5 | 1.0 | 1.1 | 1.0 | 1.0 | 1.0 | 1.3 | 1.2 | 1.4 | 2.2 | 2.1 | 2.2 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.04 | -0.17 | -0.18 | -0.22 | -0.27 | -0.3 | -0.39 | -0.39 | 0.39 | 0.39 | 0.35 | 0.82 | 0.81 | 0.88 | 0.21 | 0.44 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | 0.7 | 1.6 | -2.0 | -0.8 | -3.3 | -5.2 | -2.8 | -2.3 | -7.2 | -6.4 | -8.4 | -2.3 | -2.5 | -2.1 |
Bud | 0.3 | 2.2 | 0.7 | 0.9 | -0.7 | 0.2 | -1.1 | -0.9 | -2.4 | -2.8 | -4.3 | -5.0 | -2.4 | -2.0 | -2.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.5 | -4.1 | -4.0 | -6.1 | -7.5 | -6.4 | -7.6 | -6.1 | -6.9 | -1.6 | 2.3 | 0.6 | 3.5 | 4.5 | 4.5 |
Cytoplasm | -2.1 | -2.2 | -5.3 | -5.1 | -6.5 | -4.8 | -5.3 | -6.8 | -6.8 | 9.0 | 7.1 | 7.3 | -2.9 | -4.0 | -4.1 |
Endoplasmic Reticulum | 0.2 | 1.7 | 2.5 | 0.6 | 3.8 | 1.1 | 0 | 0.5 | 2.6 | -1.9 | -0.6 | -0.9 | -0.8 | -2.1 | -0.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.7 | 5.0 | 11.1 | 12.5 | 12.8 | 13.3 | 15.4 | 15.4 | 15.5 | -3.4 | -3.5 | -3.3 | -0.8 | 1.6 | -1.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0995 | 5.5439 | 4.5785 | 4.396 | 3.9358 | 4.7201 | 4.4634 | 5.2898 | 5.6366 | 5.3752 | 4.6014 | 5.3081 | 3.5969 | 4.9292 | 4.0918 | 4.0197 | 4.2736 | 4.2176 |
Actin | 0.021 | 0.036 | 0.0371 | 0.0444 | 0.028 | 0.0183 | 0.1026 | 0.0414 | 0.047 | 0.0554 | 0.0766 | 0.0412 | 0.0406 | 0.0136 | 0.0256 | 0.0157 | 0.0639 | 0.0359 |
Bud | 0.0005 | 0.0004 | 0.0007 | 0.0011 | 0.0013 | 0.0004 | 0.001 | 0.0013 | 0.0018 | 0.0043 | 0.0005 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0008 | 0.0007 | 0.0009 |
Bud Neck | 0.005 | 0.0107 | 0.0268 | 0.07 | 0.0081 | 0.0251 | 0.0094 | 0.0056 | 0.019 | 0.0245 | 0.0097 | 0.0238 | 0.0086 | 0.0056 | 0.0424 | 0.0497 | 0.0519 | 0.0453 |
Bud Periphery | 0.0024 | 0.0016 | 0.0036 | 0.007 | 0.0088 | 0.0036 | 0.0026 | 0.0052 | 0.0045 | 0.0176 | 0.0021 | 0.0018 | 0.0036 | 0.0014 | 0.0031 | 0.0069 | 0.0035 | 0.0058 |
Bud Site | 0.0153 | 0.0834 | 0.0463 | 0.0509 | 0.0067 | 0.0019 | 0.0118 | 0.051 | 0.0386 | 0.0275 | 0.0029 | 0.0015 | 0.0191 | 0.1095 | 0.0375 | 0.0105 | 0.0064 | 0.0029 |
Cell Periphery | 0.1764 | 0.1194 | 0.161 | 0.1828 | 0.0948 | 0.1587 | 0.1016 | 0.087 | 0.075 | 0.1278 | 0.0952 | 0.0689 | 0.2216 | 0.1466 | 0.1896 | 0.2146 | 0.08 | 0.1425 |
Cytoplasm | 0.736 | 0.7246 | 0.6775 | 0.6301 | 0.7612 | 0.7448 | 0.6131 | 0.7697 | 0.7453 | 0.6781 | 0.5551 | 0.7908 | 0.6497 | 0.7018 | 0.6785 | 0.6731 | 0.7746 | 0.7489 |
Cytoplasmic Foci | 0.0026 | 0.0031 | 0.0028 | 0.0011 | 0.0141 | 0.0018 | 0.0268 | 0.0045 | 0.006 | 0.0112 | 0.0239 | 0.0062 | 0.0071 | 0.0011 | 0.0016 | 0.001 | 0.0045 | 0.0016 |
Eisosomes | 0.0029 | 0.0017 | 0.0019 | 0.0012 | 0.001 | 0.002 | 0.0062 | 0.0008 | 0.0007 | 0.0007 | 0.0011 | 0.0007 | 0.0047 | 0.0011 | 0.0029 | 0.002 | 0.0016 | 0.0023 |
Endoplasmic Reticulum | 0.0089 | 0.0051 | 0.0083 | 0.0052 | 0.0146 | 0.0061 | 0.0235 | 0.0116 | 0.0156 | 0.0134 | 0.0096 | 0.0158 | 0.0075 | 0.0064 | 0.0074 | 0.0213 | 0.005 | 0.0066 |
Endosome | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0117 | 0.0001 | 0.0164 | 0.0009 | 0.0107 | 0.0043 | 0.065 | 0.0124 | 0.0019 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0002 |
Golgi | 0.0012 | 0.0021 | 0.0015 | 0.0006 | 0.0035 | 0.0008 | 0.0156 | 0.0026 | 0.0044 | 0.0052 | 0.0593 | 0.011 | 0.0027 | 0.0008 | 0.0012 | 0.001 | 0.0031 | 0.0015 |
Lipid Particles | 0.0025 | 0.001 | 0.0011 | 0.0004 | 0.0122 | 0.0006 | 0.0124 | 0.0038 | 0.001 | 0.0019 | 0.0501 | 0.0029 | 0.0062 | 0.0005 | 0.001 | 0.001 | 0.001 | 0.0006 |
Mitochondria | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0036 | 0.0003 | 0.0032 | 0.0014 | 0.0248 | 0.0049 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0002 |
None | 0.0199 | 0.0089 | 0.0289 | 0.0031 | 0.0071 | 0.0346 | 0.0308 | 0.0118 | 0.0205 | 0.0115 | 0.0045 | 0.0016 | 0.0182 | 0.0097 | 0.007 | 0.0007 | 0.0024 | 0.0031 |
Nuclear Periphery | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0017 | 0.0001 | 0.003 | 0.0005 | 0.0014 | 0.0017 | 0.0011 | 0.0072 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0002 |
Nucleolus | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Nucleus | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0017 | 0.0002 | 0.0024 | 0.0004 | 0.0007 | 0.0016 | 0.0004 | 0.0013 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0005 |
Peroxisomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0066 | 0 | 0.0089 | 0.0002 | 0.001 | 0.0046 | 0.0117 | 0.0013 | 0.0048 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0108 | 0.0001 | 0.0044 | 0.0002 | 0.0002 | 0.0056 | 0.0014 | 0.0045 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Vacuole | 0.002 | 0.0006 | 0.0009 | 0.0009 | 0.0037 | 0.0006 | 0.0029 | 0.0009 | 0.0027 | 0.0012 | 0.0031 | 0.0009 | 0.0011 | 0.0004 | 0.0006 | 0.0008 | 0.0003 | 0.0005 |
Vacuole Periphery | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0018 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.9843 | 14.6455 | 17.9316 | 26.6778 | 24.9587 | 25.3798 | 22.5823 | 25.4859 | 29.0849 | 32.1838 |
Translational Efficiency | 0.9628 | 1.0763 | 0.7677 | 0.8999 | 0.6334 | 0.9539 | 0.7883 | 0.7564 | 0.8388 | 0.6352 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
299 | 100 | 863 | 1383 | 1646 | 1734 | 147 | 894 | 1945 | 1834 | 1010 | 2277 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 852.78 | 1034.66 | 1112.21 | 1150.41 | 870.71 | 925.17 | 1243.23 | 1128.95 | 867.95 | 931.14 | 1131.28 | 1141.98 |
Standard Deviation | 135.88 | 146.44 | 153.22 | 160.00 | 115.01 | 110.92 | 144.30 | 172.70 | 118.63 | 115.84 | 158.83 | 165.43 |
Intensity Change Log 2 | — | 0.278911 | 0.383184 | 0.431903 | — | 0.087526 | 0.513829 | 0.374717 | — | 0.185396 | 0.450664 | 0.403296 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.036458 | 0.091571 | 0.081541 | 0.154336* | 0.045894 | 0.067087 | 0.106173* | 0.113095* | 0.044443 | 0.068422 | 0.085126 | 0.138144* |
Bud Neck | 0.022212 | 0.075605 | 0.081173 | 0.037013 | 0.062804 | 0.084770 | 0.083110 | 0.029361 | 0.056564 | 0.084271 | 0.081455 | 0.034008 |
Bud Site | 0.135649* | 0.174394* | 0.158300* | 0.170304* | 0.100370* | 0.099741 | 0.189433* | 0.137489* | 0.105793* | 0.103812* | 0.162831* | 0.157420* |
Cell Periphery | 0.582992* | 0.200981* | 0.448679* | 0.375698* | 0.261695* | 0.100171* | 0.365570* | 0.242432* | 0.311087* | 0.105668* | 0.436583* | 0.323375* |
Cytoplasm | 0.108077* | 0.252383* | 0.089231 | 0.128759* | 0.359979* | 0.434702* | 0.060950 | 0.214546* | 0.321255* | 0.424761* | 0.085114 | 0.162441* |
Cytoplasmic Foci | 0.012170 | 0.033236 | 0.002155 | 0.003135 | 0.021982 | 0.029833 | 0.005273 | 0.044060 | 0.020474 | 0.030018 | 0.002608 | 0.019203 |
Eisosomes | 0.007290 | 0.044843 | 0.039863 | 0.058468 | 0.064567 | 0.090007 | 0.062541 | 0.043738 | 0.055762 | 0.087544 | 0.043164 | 0.052684 |
Endoplasmic Reticulum | 0.022917 | 0.035095 | 0.059467 | 0.013578 | 0.015400 | 0.005960 | 0.053028 | 0.009148 | 0.016556 | 0.007549 | 0.058530 | 0.011839 |
Endosome | 0.003114 | 0.005678 | 0.001823 | 0.001895 | 0.003647 | 0.003547 | 0.001451 | 0.010364 | 0.003565 | 0.003664 | 0.001769 | 0.005220 |
Golgi | 0.005331 | 0.011516 | 0.002366 | 0.002249 | 0.011373 | 0.011627 | 0.002462 | 0.008004 | 0.010444 | 0.011621 | 0.002380 | 0.004509 |
Lipid Particles | 0.004511 | 0.007198 | 0.006967 | 0.006010 | 0.005929 | 0.006791 | 0.004992 | 0.009700 | 0.005711 | 0.006813 | 0.006679 | 0.007459 |
Mitochondria | 0.029244 | 0.002631 | 0.001002 | 0.001654 | 0.006182 | 0.005081 | 0.000125 | 0.004714 | 0.009727 | 0.004948 | 0.000874 | 0.002855 |
Mitotic Spindle | 0.000267 | 0.000201 | 0.000372 | 0.000838 | 0.000570 | 0.002037 | 0.000199 | 0.001943 | 0.000524 | 0.001937 | 0.000347 | 0.001272 |
None | 0.000372 | 0.000074 | 0.000130 | 0.000150 | 0.000390 | 0.000787 | 0.000031 | 0.000152 | 0.000388 | 0.000748 | 0.000116 | 0.000150 |
Nuclear Periphery | 0.000150 | 0.000065 | 0.000217 | 0.000193 | 0.000095 | 0.000060 | 0.000116 | 0.000149 | 0.000103 | 0.000061 | 0.000202 | 0.000175 |
Nuclear Periphery Foci | 0.000696 | 0.001531 | 0.001264 | 0.000961 | 0.000462 | 0.000421 | 0.001853 | 0.001275 | 0.000498 | 0.000482 | 0.001349 | 0.001084 |
Nucleolus | 0.001877 | 0.000048 | 0.000027 | 0.000017 | 0.000062 | 0.000107 | 0.000011 | 0.000065 | 0.000341 | 0.000104 | 0.000025 | 0.000036 |
Nucleus | 0.012196 | 0.010693 | 0.004412 | 0.007206 | 0.007734 | 0.018367 | 0.007586 | 0.010921 | 0.008420 | 0.017949 | 0.004874 | 0.008665 |
Peroxisomes | 0.009308 | 0.010178 | 0.004222 | 0.001908 | 0.021347 | 0.014551 | 0.000753 | 0.005099 | 0.019496 | 0.014313 | 0.003717 | 0.003160 |
Vacuole | 0.003601 | 0.041566 | 0.016645 | 0.034459 | 0.008917 | 0.023963 | 0.054287 | 0.109943 | 0.008100 | 0.024923 | 0.022124 | 0.064095 |
Vacuole Periphery | 0.001567 | 0.000512 | 0.000146 | 0.001172 | 0.000599 | 0.000387 | 0.000057 | 0.003804 | 0.000747 | 0.000394 | 0.000133 | 0.002205 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.72 | -6.80 | -16.05 | -4.24 | -10.52 | -6.06 | -4.94 | -10.39 | -6.79 | -0.35 | -7.15 | -8.88 | -20.66 | -14.29 | -9.06 |
Bud Neck | -4.16 | -11.08 | -3.96 | 3.08 | 9.64 | -5.59 | -2.08 | 10.72 | 16.18 | 5.76 | -7.57 | -5.55 | 8.72 | 16.10 | 11.73 |
Bud Site | -1.75 | -1.83 | -2.93 | 0.17 | -1.54 | 0.15 | -5.23 | -5.13 | -5.33 | 3.06 | 0.44 | -8.47 | -9.85 | -10.45 | 0.87 |
Cell Periphery | 11.11 | 5.90 | 9.41 | -6.05 | 6.17 | 21.60* | -4.46 | 2.27 | -12.55 | 5.35 | 26.08 | -11.41 | -0.83 | -28.49 | 10.98 |
Cytoplasm | -6.33 | 1.82 | -2.09 | 5.80 | -6.54 | -9.21 | 31.97 | 14.94 | 23.69 | -15.05 | -13.28 | 34.04 | 22.67 | 39.30 | -13.96 |
Cytoplasmic Foci | -2.67 | 5.04 | 4.45 | 3.91 | -2.25 | -3.58 | 8.11 | -6.40 | -4.20 | -11.21 | -4.68 | 12.96 | 0.38 | 4.98 | -12.09 |
Eisosomes | -4.44 | -15.46 | -20.85 | -1.55 | -6.22 | -6.99 | 0.30 | 6.44 | 12.21 | 2.73 | -9.39 | 4.63 | 1.45 | 10.90 | -3.44 |
Endoplasmic Reticulum | -1.16 | -6.78 | 2.49 | 2.15 | 10.84 | 7.78 | -4.38 | 4.54 | -2.75 | 5.12 | 6.83 | -10.94 | 3.64 | -3.77 | 12.36 |
Endosome | -1.20 | 2.04 | 1.65 | 1.79 | -0.13 | 0.17 | 4.83 | -5.13 | -5.28 | -7.04 | -0.24 | 4.43 | -2.46 | -2.33 | -5.69 |
Golgi | -1.93 | 1.97 | 1.94 | 3.23 | 0.22 | -0.22 | 9.74 | 2.11 | 2.28 | -3.49 | -1.08 | 10.49 | 5.98 | 6.96 | -2.90 |
Lipid Particles | -0.96 | -0.94 | -0.57 | 0.79 | 0.85 | -1.60 | 0.73 | -3.42 | -2.66 | -3.06 | -1.77 | -1.28 | -2.36 | -1.08 | -1.12 |
Mitochondria | 5.05 | 5.52 | 5.35 | 0.70 | -1.00 | 1.21 | 11.03 | 1.16 | 0.35 | -3.47 | 4.11 | 9.44 | 6.16 | 2.29 | -2.99 |
Mitotic Spindle | 1.10 | -2.46 | -1.69 | -1.88 | -1.39 | -1.82 | 1.13 | -1.88 | 0.18 | -2.66 | -1.88 | 0.64 | -1.76 | 0.91 | -2.81 |
None | 1.79 | 1.47 | 1.34 | -2.07 | -0.54 | -2.18 | 7.12 | 4.13 | 3.67 | -3.57 | -2.07 | 5.32 | 4.42 | 3.67 | -1.23 |
Nuclear Periphery | 1.43 | -1.12 | -0.58 | -2.89 | 0.55 | 5.86 | -1.57 | -2.68 | -4.37 | -1.35 | 4.06 | -7.25 | -2.49 | -4.15 | 0.94 |
Nuclear Periphery Foci | -1.72 | -1.63 | -0.81 | 1.43 | 1.48 | 1.12 | -5.39 | -7.43 | -7.68 | 1.91 | 0.21 | -6.01 | -5.94 | -6.56 | 1.48 |
Nucleolus | 1.29 | 1.31 | 1.32 | 2.39 | 4.64 | -6.54 | 15.27 | -0.10 | 2.53 | -3.37 | 1.09 | 1.46 | 1.41 | 8.04 | -1.70 |
Nucleus | 0.35 | 1.56 | 1.00 | 1.50 | -4.69 | -12.13 | 0.02 | -4.67 | 7.41 | -2.27 | -8.17 | 3.71 | -0.37 | 10.93 | -7.13 |
Peroxisomes | -0.37 | 3.54 | 5.40 | 4.39 | 4.73 | 4.30 | 16.11 | 11.78 | 8.99 | -7.63 | 3.65 | 13.48 | 14.53 | 12.45 | 1.25 |
Vacuole | -4.73 | -5.75 | -11.07 | 0.76 | -8.69 | -12.06 | -5.85 | -17.29 | -14.77 | -6.34 | -13.17 | -9.20 | -20.39 | -13.96 | -14.30 |
Vacuole Periphery | 2.82 | 4.73 | 0.80 | -1.43 | -2.55 | 2.19 | 6.78 | -2.75 | -2.94 | -3.24 | 3.61 | 7.47 | -2.76 | -3.49 | -4.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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