Standard name
Human Ortholog
Description Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.1 0.12 0.13 0.11 0.15 0.14 0.09 0.12 0.15 0.13 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0.08 0.05 0.06 0.06 0.09 0.1 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.14 0.17 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.12 0.06 0.07 0.08 0.06 0.1 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.18 0 0 0 0.09 0.09 0 0.15 0.12 0.18 0.09 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.25 0.28 0.25 0.14 0.1 0.1 0.1 0.12 0.11 0.08 0.07 0.09 0.1 0.06 0.1 0.09 0.11 0.12 0.06 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.53 0.34 0.41 0.3 0.43 0.35 0.4 0.49 0.49 0.46 0.44 0.65 0.55 0.52 0.69 0.59 0.6 0.44 0.46 0.38 0.22 0.18 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
SpindlePole 0.4 0.38 0.72 0.77 0.81 0.78 0.72 0.78 0.74 0.72 0.66 0.75 0.49 0.6 0.51 0.64 0.61 0.6 0.36 0.27 0.4 0.62 0.54 0.5
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 1 1 5 8 3 9 6 8 10 12 13 0 1 1 0 0 0 0 3 0 1 2 4
Bud Neck 3 1 26 22 34 28 51 46 24 34 36 37 14 11 13 3 5 1 2 2 5 1 7 11
Bud Site 2 2 2 4 10 19 17 21 15 26 24 19 3 1 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 8 6 13 4 11 11 3 7 7 9 21 30 38 15 12 12 1 0 1 0 1 1
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 4 1 2 0 0 0 0 1 1
Endosome 2 3 4 0 1 2 0 0 0 0 0 1 9 9 7 13 10 18 9 18 23 10 20 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 1 0 1 4
Mitochondria 13 40 7 8 1 22 29 11 41 34 44 25 13 3 3 5 3 7 1 3 1 0 2 4
Nucleus 31 64 64 26 27 25 32 40 30 22 17 25 24 13 24 20 18 19 8 26 10 4 6 4
Nuclear Periphery 1 2 1 0 0 0 1 0 0 1 1 0 1 1 0 1 1 0 0 2 2 0 0 0
Nucleolus 84 119 88 78 80 108 115 135 131 143 112 124 160 119 120 148 101 92 62 122 107 37 40 51
Peroxisomes 0 0 0 1 2 0 0 3 0 0 0 0 7 4 12 1 10 2 1 1 1 1 3 4
SpindlePole 50 86 186 147 218 196 239 265 201 208 159 214 122 131 117 137 104 92 50 71 113 107 118 123
Vac/Vac Membrane 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0 6 5 8 1 2 5 3 5 5
Unique Cell Count 125 225 258 190 270 252 332 340 270 289 241 285 247 218 229 213 171 153 142 264 283 174 218 247
Labelled Cell Count 190 322 389 297 396 408 505 538 453 485 412 467 374 324 336 355 273 253 142 264 283 174 218 247


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.1 5.5 5.0 5.5 4.2 4.4 4.7 3.9 3.7 3.5 4.1 5.1 5.6 5.1 8.9 9.6 8.7 5.2 5.4 5.5
Std Deviation (1e-4) 0.8 0.8 1.0 1.3 1.4 0.7 1.0 1.1 0.8 0.7 0.8 1.0 1.1 1.5 1.4 1.9 2.6 2.3 1.6 1.6 1.6
Intensity Change (Log2) – – – -0.15 0.0 -0.38 -0.32 -0.22 -0.51 -0.59 -0.67 -0.43 -0.12 0.0 -0.11 0.69 0.79 0.65 -0.1 -0.03 -0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.5 0.9 0 0 0 0 0 0 0 -1.8 -2.0 -1.8 -3.9 -2.8 -3.7
Bud Site 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.0 -1.1 0.1 0.1 -1.6 -0.5 -0.1 0 2.6 4.3 5.1 2.0 1.9 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.5 1.7 1.1 2.6 2.5 4.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 0 0 0 0 0 0 0 0 1.5 0 0 -0.3 0 1.0
Nucleus -2.9 -4.5 -4.4 -5.0 -4.2 -4.1 -5.5 -5.4 -5.0 -4.5 -5.6 -4.1 -4.4 -3.7 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 -1.1 0 0 0 0 0 0 0 6.9 4.5 4.1 7.6 5.1 5.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 3.7 0 0 0
SpindlePole 1.3 2.3 0 0 0 0 0 0 0 -5.2 -2.8 -4.8 -1.8 -2.4 -2.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1017 0.3342 0.2302 0.2358 -0.3234 0.4562 1.3231 1.4171 1.3757 1.5035 1.1863 1.0768 0.8522 0.7876 0.9153 1.5796 0.5098 0.6434
Actin 0.0493 0 0.0197 0.0004 0.0001 0.0235 0.0081 0 0.0027 0.0002 0 0.0007 0.016 0 0.0189 0.0677 0 0.0013
Bud 0.0007 0.0001 0.0002 0 0.0003 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0003 0.0001 0.0001 0.0003 0 0
Bud Neck 0.0053 0.0021 0.0017 0.0017 0.0147 0.0019 0.0023 0.0017 0.0015 0.0056 0.0025 0.0008 0.0021 0.0016 0.0017 0.0013 0.0025 0.0011
Bud Periphery 0.0017 0.0002 0.0002 0.0001 0.0007 0.0002 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0.0006 0.0002 0.0002 0.0006 0.0001 0.0001
Bud Site 0.003 0.0019 0.0022 0.0002 0.0018 0.0004 0.0009 0.0006 0.0013 0.0008 0.0003 0.0001 0.0021 0.0013 0.0008 0.0011 0.0002 0.0002
Cell Periphery 0.001 0.0005 0.0004 0.0001 0.0007 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0002 0.0003 0 0.0001
Cytoplasm 0.0046 0.0002 0.001 0.0012 0.0028 0.0004 0.0011 0 0.0001 0.0001 0 0.0001 0.0032 0.0001 0.003 0.0011 0 0
Cytoplasmic Foci 0.0508 0.0236 0.0226 0.0166 0.0097 0.0158 0.0355 0.0144 0.0205 0.0278 0.0128 0.0136 0.0242 0.0164 0.0099 0.0175 0.0033 0.0088
Eisosomes 0.001 0 0.0005 0.0001 0 0.0002 0.0001 0 0.0001 0 0 0 0.0003 0 0.0002 0.0007 0 0
Endoplasmic Reticulum 0.0045 0.0001 0.0009 0.0002 0.0004 0.0024 0.0011 0 0.0001 0.0001 0 0.0003 0.0027 0 0.0038 0.0036 0 0.0002
Endosome 0.0266 0.0052 0.004 0.0036 0.0053 0.0089 0.0156 0.0006 0.0041 0.0031 0.0006 0.0054 0.0127 0.0023 0.0064 0.0224 0.0002 0.0015
Golgi 0.0085 0.0017 0.0059 0.0024 0.0014 0.0088 0.0082 0.0007 0.005 0.0036 0.0013 0.0067 0.0049 0.0013 0.005 0.0062 0.0005 0.0016
Lipid Particles 0.0829 0.0756 0.065 0.1542 0.0436 0.0696 0.0928 0.0379 0.0693 0.1123 0.0708 0.0394 0.0773 0.0366 0.0367 0.0374 0.0065 0.0327
Mitochondria 0.0056 0.0003 0.0022 0.0005 0.0009 0.022 0.0045 0.0001 0.0094 0.0007 0.0001 0.0028 0.019 0.0002 0.0009 0.0076 0.0001 0.0002
None 0.0064 0.0002 0.013 0.0064 0.0016 0.0005 0.0016 0 0.0001 0.0001 0 0.0001 0.0074 0.0001 0.0021 0.0017 0 0.0001
Nuclear Periphery 0.0145 0.0015 0.0096 0.0063 0.0039 0.006 0.0054 0.0002 0.0013 0.001 0.0019 0.0067 0.0099 0.0003 0.012 0.0164 0.001 0.0044
Nucleolus 0.3333 0.5331 0.5951 0.3946 0.549 0.3378 0.4987 0.657 0.6209 0.6886 0.5114 0.399 0.4743 0.5701 0.6772 0.7063 0.4177 0.4126
Nucleus 0.0222 0.0363 0.0483 0.1181 0.0721 0.0288 0.0228 0.0545 0.0263 0.0461 0.0196 0.0191 0.0319 0.0733 0.053 0.0476 0.0245 0.0309
Peroxisomes 0.0167 0.0026 0.005 0.0024 0.0045 0.0055 0.0058 0.0012 0.0034 0.003 0.0014 0.0008 0.0081 0.002 0.0113 0.0066 0.0005 0.001
Punctate Nuclear 0.3457 0.3021 0.1996 0.2899 0.2817 0.4664 0.2922 0.2302 0.2315 0.1046 0.376 0.5022 0.3006 0.2919 0.1556 0.0506 0.5426 0.5029
Vacuole 0.0131 0.0117 0.0021 0.0006 0.0043 0.0004 0.0018 0.0005 0.0013 0.0013 0.0007 0.0008 0.0016 0.0019 0.0007 0.0017 0.0001 0.0003
Vacuole Periphery 0.0026 0.001 0.0009 0.0003 0.0006 0.0004 0.0011 0.0001 0.0008 0.0004 0.0001 0.0012 0.0007 0.0002 0.0005 0.0015 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.005 14.0546 4.4245 5.5216 8.6479 7.6186 14.6412 10.7537 7.3581 13.0699
Translational Efficiency 1.3409 1.1636 1.1427 1.2861 1.4062 1.3123 0.907 0.8326 1.0516 0.8279

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1795 155 459 1362 1490 1638 128 98 3285 1793 587 1460

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.97 842.58 1002.49 982.70 679.45 768.71 1018.05 1069.44 687.93 775.10 1005.88 988.52
Standard Deviation 80.36 119.98 139.89 136.12 91.87 106.55 138.46 120.17 86.12 109.75 139.73 136.84
Intensity Change Log 2 — 0.277863 0.528565 0.499800 — 0.178072 0.583369 0.654416 — 0.229393 0.555918 0.578306

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000234 0.000751 0.000497 0.000449 0.000493 0.000545 0.000599 0.000475 0.000352 0.000563 0.000519 0.000451
Bud Neck 0.010416 0.010371 0.006612 0.014860 0.006289 0.010052 0.008489 0.012714 0.008544 0.010080 0.007021 0.014716
Bud Site 0.003798 0.002740 0.001942 0.005859 0.002368 0.002773 0.002790 0.004776 0.003149 0.002770 0.002127 0.005786
Cell Periphery 0.000181 0.000096 0.000042 0.000071 0.000201 0.000170 0.000052 0.000061 0.000190 0.000163 0.000044 0.000071
Cytoplasm 0.009899 0.002088 0.000149 0.000819 0.007906 0.004044 0.000227 0.000494 0.008995 0.003875 0.000166 0.000797
Cytoplasmic Foci 0.016321 0.004539 0.000977 0.003443 0.019434 0.011876 0.001678 0.001983 0.017733 0.011242 0.001130 0.003345
Eisosomes 0.000167 0.000163 0.000080 0.000068 0.000207 0.000176 0.000110 0.000073 0.000185 0.000175 0.000087 0.000068
Endoplasmic Reticulum 0.002649 0.004495 0.001537 0.000934 0.004748 0.004413 0.002167 0.000918 0.003601 0.004421 0.001675 0.000933
Endosome 0.010692 0.010021 0.000613 0.003474 0.010353 0.011424 0.000791 0.001091 0.010538 0.011302 0.000652 0.003314
Golgi 0.009195 0.005261 0.001389 0.003775 0.012836 0.008572 0.001658 0.001401 0.010846 0.008285 0.001447 0.003616
Lipid Particles 0.005580 0.006125 0.000275 0.000474 0.014904 0.009290 0.000423 0.000290 0.009809 0.009016 0.000307 0.000462
Mitochondria 0.003483 0.003179 0.000898 0.003171 0.005159 0.010254 0.000627 0.001489 0.004243 0.009643 0.000839 0.003058
Mitotic Spindle 0.081095 0.296540 0.279785 0.310237 0.096093 0.167032 0.275976 0.390571 0.087898 0.178228 0.278955 0.315630
None 0.009381 0.003072 0.000434 0.000901 0.010667 0.004468 0.000465 0.000713 0.009965 0.004347 0.000441 0.000888
Nuclear Periphery 0.000896 0.001849 0.001018 0.002967 0.001438 0.002250 0.001063 0.001055 0.001142 0.002216 0.001028 0.002838
Nuclear Periphery Foci 0.088804 0.121829 0.011049 0.010154 0.180652 0.122374 0.017386 0.008506 0.130465 0.122327 0.012431 0.010044
Nucleolus 0.611303 0.323145 0.507117 0.500492 0.481172 0.446542 0.501653 0.400718 0.552279 0.435875 0.505926 0.493795
Nucleus 0.118848 0.180011 0.165001 0.115481 0.122178 0.163189 0.156905 0.145585 0.120359 0.164643 0.163235 0.117502
Peroxisomes 0.015142 0.020991 0.018530 0.013902 0.020894 0.015301 0.024064 0.020689 0.017751 0.015793 0.019737 0.014358
Vacuole 0.001346 0.001925 0.001583 0.005026 0.001365 0.003413 0.002265 0.004607 0.001355 0.003285 0.001731 0.004998
Vacuole Periphery 0.000569 0.000810 0.000471 0.003440 0.000642 0.001841 0.000613 0.001790 0.000602 0.001752 0.000502 0.003330

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.20 -6.86 -4.70 1.96 1.95 -1.83 -2.68 -1.62 -0.60 0.96 -6.25 -6.26 -3.16 1.00 2.27
Bud Neck -0.86 0.74 -9.03 -4.39 -9.22 -5.02 -3.23 -3.95 -2.98 -2.29 -2.55 -2.15 -11.99 -9.28 -9.13
Bud Site -0.76 1.10 -5.28 -1.37 -5.86 -1.23 -2.50 -3.24 -3.06 -1.72 0.31 -1.52 -6.76 -6.69 -5.47
Cell Periphery 3.75 6.93 5.04 1.12 -5.20 1.62 9.97 6.52 4.92 -1.50 1.36 11.51 8.53 6.64 -5.24
Cytoplasm 4.77 7.42 6.07 0.80 -2.98 2.69 4.29 5.10 3.77 -0.02 4.63 9.22 7.51 3.49 -2.36
Cytoplasmic Foci 8.27 11.54 9.09 0.09 -6.05 4.04 9.43 7.54 4.60 -0.85 5.33 15.57 12.38 6.08 -5.54
Eisosomes 0.53 14.80 24.57 7.70 3.55 5.51 11.06 16.54 12.42 3.58 2.60 20.32 35.26 23.44 5.15
Endoplasmic Reticulum -4.96 3.50 13.74 8.91 4.88 0.93 8.11 16.32 15.25 4.54 -5.60 9.21 23.15 21.54 6.28
Endosome 0.29 9.96 7.08 1.30 -4.35 -0.47 7.16 8.53 8.71 0.15 -0.42 13.30 9.49 7.40 -3.89
Golgi 4.36 12.21 6.91 2.69 -2.21 3.86 12.26 12.26 9.30 0.62 3.45 17.35 9.65 5.71 -2.15
Lipid Particles -0.34 7.07 6.91 3.59 -1.14 3.17 10.19 10.23 10.00 0.53 0.37 12.32 12.19 10.35 -1.03
Mitochondria 0.16 6.33 0.59 0.10 -4.01 -3.57 6.51 4.05 6.16 -3.24 -4.49 8.66 2.15 5.36 -4.30
Mitotic Spindle -7.16 -12.35 -21.66 -1.05 -1.72 -8.08 -6.33 -7.70 -5.85 -2.51 -11.44 -13.79 -23.30 -12.58 -2.34
None 3.93 8.78 8.17 1.57 -2.13 4.38 8.09 7.76 6.33 -1.38 5.52 11.94 11.13 6.57 -2.14
Nuclear Periphery -3.66 -1.89 -3.48 -1.58 -2.87 -6.05 1.06 0.60 3.75 -0.15 -8.79 -0.41 -3.03 -0.72 -2.87
Nuclear Periphery Foci -1.06 19.11 20.88 6.60 1.31 7.33 24.96 25.12 19.79 1.97 2.04 31.28 33.72 24.19 2.01
Nucleolus 11.91 7.86 11.04 -6.29 0.49 3.41 -0.14 3.48 2.35 2.90 13.21 4.76 7.40 -3.77 0.96
Nucleus -4.26 -7.73 -1.52 3.74 6.57 -8.74 -3.41 -1.54 1.04 1.00 -11.17 -8.33 -1.49 8.13 6.84
Peroxisomes -1.18 -2.17 0.37 1.30 2.60 3.99 -1.12 -0.34 -2.02 0.62 1.82 -1.52 2.63 0.46 3.28
Vacuole -1.80 -3.45 -5.45 -4.09 -3.63 -3.51 -1.82 -2.16 -1.31 -0.65 -3.75 -3.62 -5.75 -3.18 -3.25
Vacuole Periphery -2.04 -0.87 -4.26 -3.55 -4.18 -4.51 -1.16 -1.70 -0.03 -1.53 -4.89 -0.88 -4.34 -2.00 -4.25
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
Localization
Cell Percentages nucleolus (47%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Tof2

Tof2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tof2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available