Standard name
Human Ortholog
Description Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.1 0.12 0.13 0.11 0.15 0.14 0.09 0.12 0.15 0.13 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0.08 0.05 0.06 0.06 0.09 0.1 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.14 0.17 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.12 0.06 0.07 0.08 0.06 0.1 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.18 0 0 0 0.09 0.09 0 0.15 0.12 0.18 0.09 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.25 0.28 0.25 0.14 0.1 0.1 0.1 0.12 0.11 0.08 0.07 0.09 0.1 0.06 0.1 0.09 0.11 0.12 0.06 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.53 0.34 0.41 0.3 0.43 0.35 0.4 0.49 0.49 0.46 0.44 0.65 0.55 0.52 0.69 0.59 0.6 0.44 0.46 0.38 0.22 0.18 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
SpindlePole 0.4 0.38 0.72 0.77 0.81 0.78 0.72 0.78 0.74 0.72 0.66 0.75 0.49 0.6 0.51 0.64 0.61 0.6 0.36 0.27 0.4 0.62 0.54 0.5
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 1 1 5 8 3 9 6 8 10 12 13 0 1 1 0 0 0 0 3 0 1 2 4
Bud Neck 3 1 26 22 34 28 51 46 24 34 36 37 14 11 13 3 5 1 2 2 5 1 7 11
Bud Site 2 2 2 4 10 19 17 21 15 26 24 19 3 1 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 8 6 13 4 11 11 3 7 7 9 21 30 38 15 12 12 1 0 1 0 1 1
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 4 1 2 0 0 0 0 1 1
Endosome 2 3 4 0 1 2 0 0 0 0 0 1 9 9 7 13 10 18 9 18 23 10 20 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 1 0 1 4
Mitochondria 13 40 7 8 1 22 29 11 41 34 44 25 13 3 3 5 3 7 1 3 1 0 2 4
Nucleus 31 64 64 26 27 25 32 40 30 22 17 25 24 13 24 20 18 19 8 26 10 4 6 4
Nuclear Periphery 1 2 1 0 0 0 1 0 0 1 1 0 1 1 0 1 1 0 0 2 2 0 0 0
Nucleolus 84 119 88 78 80 108 115 135 131 143 112 124 160 119 120 148 101 92 62 122 107 37 40 51
Peroxisomes 0 0 0 1 2 0 0 3 0 0 0 0 7 4 12 1 10 2 1 1 1 1 3 4
SpindlePole 50 86 186 147 218 196 239 265 201 208 159 214 122 131 117 137 104 92 50 71 113 107 118 123
Vac/Vac Membrane 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0 6 5 8 1 2 5 3 5 5
Unique Cell Count 125 225 258 190 270 252 332 340 270 289 241 285 247 218 229 213 171 153 142 264 283 174 218 247
Labelled Cell Count 190 322 389 297 396 408 505 538 453 485 412 467 374 324 336 355 273 253 142 264 283 174 218 247


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.1 5.5 5.0 5.5 4.2 4.4 4.7 3.9 3.7 3.5 4.1 5.1 5.6 5.1 8.9 9.6 8.7 5.2 5.4 5.5
Std Deviation (1e-4) 0.8 0.8 1.0 1.3 1.4 0.7 1.0 1.1 0.8 0.7 0.8 1.0 1.1 1.5 1.4 1.9 2.6 2.3 1.6 1.6 1.6
Intensity Change (Log2) – – – -0.15 0.0 -0.38 -0.32 -0.22 -0.51 -0.59 -0.67 -0.43 -0.12 0.0 -0.11 0.69 0.79 0.65 -0.1 -0.03 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.5 0.9 0 0 0 0 0 0 0 -1.8 -2.0 -1.8 -3.9 -2.8 -3.7
Bud Site 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.0 -1.1 0.1 0.1 -1.6 -0.5 -0.1 0 2.6 4.3 5.1 2.0 1.9 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.5 1.7 1.1 2.6 2.5 4.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 0 0 0 0 0 0 0 0 1.5 0 0 -0.3 0 1.0
Nucleus -2.9 -4.5 -4.4 -5.0 -4.2 -4.1 -5.5 -5.4 -5.0 -4.5 -5.6 -4.1 -4.4 -3.7 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 -1.1 0 0 0 0 0 0 0 6.9 4.5 4.1 7.6 5.1 5.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 3.7 0 0 0
SpindlePole 1.3 2.3 0 0 0 0 0 0 0 -5.2 -2.8 -4.8 -1.8 -2.4 -2.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1017 0.3342 0.2302 0.2358 -0.3234 0.4562 1.3231 1.4171 1.3757 1.5035 1.1863 1.0768 0.8522 0.7876 0.9153 1.5796 0.5098 0.6434
Actin 0.0493 0 0.0197 0.0004 0.0001 0.0235 0.0081 0 0.0027 0.0002 0 0.0007 0.016 0 0.0189 0.0677 0 0.0013
Bud 0.0007 0.0001 0.0002 0 0.0003 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0003 0.0001 0.0001 0.0003 0 0
Bud Neck 0.0053 0.0021 0.0017 0.0017 0.0147 0.0019 0.0023 0.0017 0.0015 0.0056 0.0025 0.0008 0.0021 0.0016 0.0017 0.0013 0.0025 0.0011
Bud Periphery 0.0017 0.0002 0.0002 0.0001 0.0007 0.0002 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0.0006 0.0002 0.0002 0.0006 0.0001 0.0001
Bud Site 0.003 0.0019 0.0022 0.0002 0.0018 0.0004 0.0009 0.0006 0.0013 0.0008 0.0003 0.0001 0.0021 0.0013 0.0008 0.0011 0.0002 0.0002
Cell Periphery 0.001 0.0005 0.0004 0.0001 0.0007 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0002 0.0003 0 0.0001
Cytoplasm 0.0046 0.0002 0.001 0.0012 0.0028 0.0004 0.0011 0 0.0001 0.0001 0 0.0001 0.0032 0.0001 0.003 0.0011 0 0
Cytoplasmic Foci 0.0508 0.0236 0.0226 0.0166 0.0097 0.0158 0.0355 0.0144 0.0205 0.0278 0.0128 0.0136 0.0242 0.0164 0.0099 0.0175 0.0033 0.0088
Eisosomes 0.001 0 0.0005 0.0001 0 0.0002 0.0001 0 0.0001 0 0 0 0.0003 0 0.0002 0.0007 0 0
Endoplasmic Reticulum 0.0045 0.0001 0.0009 0.0002 0.0004 0.0024 0.0011 0 0.0001 0.0001 0 0.0003 0.0027 0 0.0038 0.0036 0 0.0002
Endosome 0.0266 0.0052 0.004 0.0036 0.0053 0.0089 0.0156 0.0006 0.0041 0.0031 0.0006 0.0054 0.0127 0.0023 0.0064 0.0224 0.0002 0.0015
Golgi 0.0085 0.0017 0.0059 0.0024 0.0014 0.0088 0.0082 0.0007 0.005 0.0036 0.0013 0.0067 0.0049 0.0013 0.005 0.0062 0.0005 0.0016
Lipid Particles 0.0829 0.0756 0.065 0.1542 0.0436 0.0696 0.0928 0.0379 0.0693 0.1123 0.0708 0.0394 0.0773 0.0366 0.0367 0.0374 0.0065 0.0327
Mitochondria 0.0056 0.0003 0.0022 0.0005 0.0009 0.022 0.0045 0.0001 0.0094 0.0007 0.0001 0.0028 0.019 0.0002 0.0009 0.0076 0.0001 0.0002
None 0.0064 0.0002 0.013 0.0064 0.0016 0.0005 0.0016 0 0.0001 0.0001 0 0.0001 0.0074 0.0001 0.0021 0.0017 0 0.0001
Nuclear Periphery 0.0145 0.0015 0.0096 0.0063 0.0039 0.006 0.0054 0.0002 0.0013 0.001 0.0019 0.0067 0.0099 0.0003 0.012 0.0164 0.001 0.0044
Nucleolus 0.3333 0.5331 0.5951 0.3946 0.549 0.3378 0.4987 0.657 0.6209 0.6886 0.5114 0.399 0.4743 0.5701 0.6772 0.7063 0.4177 0.4126
Nucleus 0.0222 0.0363 0.0483 0.1181 0.0721 0.0288 0.0228 0.0545 0.0263 0.0461 0.0196 0.0191 0.0319 0.0733 0.053 0.0476 0.0245 0.0309
Peroxisomes 0.0167 0.0026 0.005 0.0024 0.0045 0.0055 0.0058 0.0012 0.0034 0.003 0.0014 0.0008 0.0081 0.002 0.0113 0.0066 0.0005 0.001
Punctate Nuclear 0.3457 0.3021 0.1996 0.2899 0.2817 0.4664 0.2922 0.2302 0.2315 0.1046 0.376 0.5022 0.3006 0.2919 0.1556 0.0506 0.5426 0.5029
Vacuole 0.0131 0.0117 0.0021 0.0006 0.0043 0.0004 0.0018 0.0005 0.0013 0.0013 0.0007 0.0008 0.0016 0.0019 0.0007 0.0017 0.0001 0.0003
Vacuole Periphery 0.0026 0.001 0.0009 0.0003 0.0006 0.0004 0.0011 0.0001 0.0008 0.0004 0.0001 0.0012 0.0007 0.0002 0.0005 0.0015 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.005 14.0546 4.4245 5.5216 8.6479 7.6186 14.6412 10.7537 7.3581 13.0699
Translational Efficiency 1.3409 1.1636 1.1427 1.2861 1.4062 1.3123 0.907 0.8326 1.0516 0.8279

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
Localization
Cell Percentages nucleolus (47%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Tof2

Tof2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tof2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available