ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.12 0.06 0.05 0 0 0 0 0 0 0.07 0.11 0.15 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0 0 0.05 0 0.18 0.37 0.27 0.46 0.4 0.63 0.29 0 0 0 0 0 0.05 0 0 0 0 0 0.05
Nucleus 0.87 0.91 0.92 0.85 0.91 0.86 0.81 0.82 0.78 0.78 0.72 0.82 0.91 0.89 0.81 0.9 0.88 0.89 0.86 0.82 0.83 0.62 0.59 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.13 0.11 0.17 0.17 0.15 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.09 0.23 0.2 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 1 0 0 0 3 4 3 5 10 3 0 0 0 0 1 1 3 2 1 2 3 4
Bud Neck 0 0 3 0 1 0 2 1 0 4 2 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 1 0 0 0 0 10 17 11 15 15 22 13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 9 8 10 16 18 15 19 21 15 12 11 8 13 19 34 11 12 7 1 0 4 4 3 4
Endoplasmic Reticulum 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 3 1 0 1 1 0 0
Endosome 0 1 0 2 1 0 2 1 0 1 0 1 1 0 2 3 1 0 3 0 0 1 4 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 2 1 2 0 0 0 2
Mitochondria 136 4 7 7 9 59 164 155 196 181 248 122 2 1 1 2 4 11 5 1 1 2 8 15
Nucleus 341 224 181 115 294 273 358 464 332 348 284 346 164 159 180 271 247 197 346 214 185 117 172 140
Nuclear Periphery 1 1 0 0 4 4 4 3 2 2 6 0 2 0 1 8 7 4 0 0 0 0 1 2
Nucleolus 2 2 0 1 6 17 59 63 70 74 58 44 1 1 0 6 10 8 5 6 1 7 18 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 1
SpindlePole 2 0 5 3 3 2 5 11 2 11 3 8 1 2 1 1 0 1 3 3 3 3 11 15
Vac/Vac Membrane 5 15 0 0 7 3 1 2 2 4 2 3 1 0 3 12 12 7 25 28 21 44 59 64
Unique Cell Count 392 246 196 135 322 319 441 567 424 448 393 424 181 179 221 301 281 222 402 260 225 190 294 289
Labelled Cell Count 501 256 207 144 343 383 634 736 637 657 646 549 185 182 224 322 300 241 402 260 225 190 294 289


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 6.2 5.5 5.8 6.7 5.4 4.9 5.1 4.6 4.7 4.1 4.8 6.6 7.1 7.1 11.1 11.1 10.4 5.6 6.2 6.5
Std Deviation (1e-4) 0.5 1.2 1.0 1.8 1.6 1.6 1.4 1.1 1.3 1.1 1.0 1.1 1.4 1.7 2.0 2.7 5.6 3.3 1.8 1.6 1.4
Intensity Change (Log2) – – – 0.08 0.27 -0.03 -0.18 -0.12 -0.27 -0.23 -0.43 -0.21 0.26 0.36 0.36 1.01 1.01 0.91 0.02 0.16 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 0.2 -0.2 -0.4 -0.9 -0.9 -1.6 -1.4 -2.2 0.8 2.0 3.4 -0.8 -0.4 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 -0.5 4.9 0 7.0 0 0 0 0 0 0 0 0 0 0.7
Nucleus -2.1 -0.4 -2.3 -3.6 -3.5 -4.3 -4.5 -5.6 -3.5 -0.6 -1.2 -3.3 -0.9 -1.6 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.3 0 4.9 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9914 2.7755 2.6887 2.4334 2.3274 2.3979 2.7747 2.4787 2.306 2.3784 2.1462 2.4021 2.0515 1.0694 1.1782 1.5397 0.9197 1.2035
Actin 0.0097 0 0.0122 0.0176 0.006 0.0019 0 0 0 0 0 0.0006 0.0198 0 0.0062 0.0496 0.0081 0.0005
Bud 0.0002 0 0.0002 0.0005 0.0009 0.0001 0 0 0 0 0 0 0.0004 0 0.0001 0.0002 0.0004 0
Bud Neck 0.0015 0.0001 0.0003 0.0023 0.0007 0.0011 0.0003 0 0 0 0.0001 0.0003 0.0021 0.0002 0.0004 0.0003 0.0007 0.0004
Bud Periphery 0.0003 0 0.0003 0.0014 0.0013 0.0002 0.0001 0 0 0 0 0 0.0009 0 0.0001 0.0005 0.0018 0.0001
Bud Site 0.0018 0.0001 0.0009 0.0018 0.0011 0.0001 0.0001 0 0 0 0 0 0.0073 0.0001 0.0004 0.0011 0.001 0
Cell Periphery 0.0001 0 0.0001 0.0005 0.0001 0 0 0 0 0 0 0 0.0004 0 0 0.0001 0.0005 0
Cytoplasm 0.0064 0.0009 0.0131 0.0006 0.0087 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0.0138 0.0003 0.0157 0.0006 0.0009 0.0001
Cytoplasmic Foci 0.0189 0 0.0048 0.0129 0.0086 0.0005 0 0 0 0 0 0.0001 0.0052 0 0.0027 0.0027 0.0061 0.0002
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0003 0.0006 0
Endoplasmic Reticulum 0.0028 0.0001 0.0009 0.0006 0.009 0 0 0 0 0 0 0 0.0043 0 0.0002 0.001 0.0011 0.0001
Endosome 0.0257 0.0001 0.0094 0.0215 0.0691 0.0004 0.0002 0 0 0 0 0.0001 0.0108 0.0001 0.0015 0.0071 0.0043 0.0015
Golgi 0.0053 0 0.0042 0.0225 0.0267 0.0011 0 0 0 0 0 0.0001 0.0049 0 0.0008 0.0045 0.002 0.0005
Lipid Particles 0.0069 0 0.0096 0.0279 0.0106 0.0007 0 0 0 0 0 0.0003 0.012 0 0.0047 0.0121 0.012 0.0018
Mitochondria 0.0096 0.0001 0.0049 0.0124 0.0343 0.0077 0.0002 0 0 0 0.0001 0.0001 0.0093 0.0002 0.0058 0.0124 0.128 0.0014
None 0.0022 0.0003 0.0007 0.0002 0.0028 0.0001 0 0 0 0 0 0.0001 0.0101 0.0001 0.0008 0.0011 0.0042 0.0001
Nuclear Periphery 0.0094 0.0057 0.005 0.0016 0.0194 0.0009 0.0012 0.0011 0.0006 0.0001 0.0013 0.0013 0.0362 0.0025 0.0058 0.0138 0.0076 0.0029
Nucleolus 0.0058 0.0083 0.006 0.0061 0.0324 0.0316 0.0081 0.0097 0.008 0.0059 0.0249 0.0217 0.0101 0.0124 0.0073 0.0084 0.0382 0.0216
Nucleus 0.8781 0.9746 0.9153 0.82 0.7524 0.9462 0.9876 0.9874 0.9894 0.9937 0.9489 0.9697 0.8254 0.9799 0.9386 0.8717 0.7047 0.9643
Peroxisomes 0.0032 0 0.009 0.0326 0.0021 0.0056 0 0 0 0 0 0.0001 0.0076 0 0.0025 0.0061 0.0193 0.0002
Punctate Nuclear 0.0082 0.0095 0.0013 0.0016 0.0087 0.0012 0.0012 0.0015 0.002 0.0001 0.0244 0.0054 0.0107 0.004 0.0056 0.004 0.0421 0.0039
Vacuole 0.0025 0.0002 0.001 0.0134 0.0025 0.0002 0.0005 0 0 0 0 0 0.0049 0.0001 0.0007 0.0008 0.0055 0.0002
Vacuole Periphery 0.001 0.0001 0.0007 0.002 0.0026 0.0001 0.0001 0 0 0 0 0 0.0034 0.0001 0.0002 0.0017 0.0109 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9094 17.647 8.2704 20.769 9.4296 14.5378 23.947 23.1348 25.4757 17.7313
Translational Efficiency 1.7861 1.9014 2.9367 1.6714 2.498 2.7835 1.9094 1.993 1.8577 2.119

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1325 1383 3338 1991 1325 1783 96 1866 2650 3166 3434 3857

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 772.66 694.23 962.54 1188.45 669.34 789.52 1069.91 1236.21 721.00 747.89 965.54 1211.56
Standard Deviation 101.77 114.75 143.43 248.04 81.93 105.60 175.77 248.70 105.85 119.44 145.51 249.51
Intensity Change Log 2 — -0.154420 0.317013 0.621176 — 0.238237 0.676678 0.885113 — 0.041177 0.495149 0.749711

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000071 0.000438 0.000301 0.000679 0.000275 0.000376 0.000803 0.000858 0.000173 0.000403 0.000315 0.000766
Bud Neck 0.003492 0.006660 0.018742 0.028046 0.002092 0.007711 0.003332 0.028464 0.002792 0.007252 0.018311 0.028248
Bud Site 0.000843 0.002627 0.001228 0.003426 0.000830 0.002870 0.001374 0.003032 0.000837 0.002763 0.001232 0.003236
Cell Periphery 0.000082 0.000355 0.000108 0.000171 0.000088 0.000135 0.000042 0.000145 0.000085 0.000231 0.000106 0.000158
Cytoplasm 0.022217 0.043398 0.002483 0.001440 0.013910 0.028060 0.000277 0.001940 0.018063 0.034760 0.002422 0.001682
Cytoplasmic Foci 0.001886 0.004915 0.000013 0.000508 0.003062 0.007724 0.000036 0.000806 0.002474 0.006497 0.000013 0.000652
Eisosomes 0.000010 0.000026 0.000024 0.000032 0.000020 0.000023 0.000036 0.000029 0.000015 0.000024 0.000025 0.000030
Endoplasmic Reticulum 0.001413 0.004985 0.003259 0.003864 0.002527 0.004369 0.006071 0.002326 0.001970 0.004638 0.003337 0.003120
Endosome 0.000185 0.001127 0.000153 0.001258 0.000515 0.003108 0.000117 0.001266 0.000350 0.002243 0.000152 0.001262
Golgi 0.000069 0.000478 0.000031 0.001063 0.000295 0.001859 0.000042 0.000846 0.000182 0.001256 0.000031 0.000958
Lipid Particles 0.000457 0.001193 0.000022 0.000068 0.001113 0.002227 0.000082 0.000065 0.000785 0.001775 0.000024 0.000066
Mitochondria 0.001330 0.003902 0.001289 0.003969 0.002009 0.006724 0.002799 0.002887 0.001669 0.005491 0.001331 0.003445
Mitotic Spindle 0.001278 0.002810 0.001371 0.010222 0.001666 0.011381 0.000096 0.009446 0.001472 0.007637 0.001335 0.009847
None 0.003783 0.033100 0.001936 0.001768 0.006869 0.004657 0.001405 0.002272 0.005326 0.017082 0.001921 0.002012
Nuclear Periphery 0.001131 0.001336 0.000610 0.002036 0.000695 0.002247 0.000172 0.001432 0.000913 0.001849 0.000598 0.001744
Nuclear Periphery Foci 0.000169 0.001094 0.000037 0.000937 0.000804 0.001055 0.000041 0.000957 0.000486 0.001072 0.000037 0.000947
Nucleolus 0.005801 0.015009 0.001848 0.010827 0.011052 0.007145 0.004326 0.015512 0.008427 0.010580 0.001918 0.013094
Nucleus 0.954765* 0.871365* 0.965435* 0.923639* 0.950266* 0.899159* 0.975666* 0.923048* 0.952516* 0.887017* 0.965721* 0.923353*
Peroxisomes 0.000438 0.001835 0.000047 0.000193 0.000992 0.002043 0.000347 0.000362 0.000715 0.001952 0.000055 0.000274
Vacuole 0.000492 0.002847 0.000824 0.003637 0.000686 0.005058 0.002717 0.003176 0.000589 0.004092 0.000877 0.003414
Vacuole Periphery 0.000088 0.000500 0.000239 0.002219 0.000235 0.002070 0.000221 0.001133 0.000162 0.001384 0.000239 0.001694

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.08 -14.70 -14.61 -4.76 -8.95 -1.25 -4.63 -3.78 -3.29 -0.58 -5.43 -4.94 -7.59 -4.78 -6.01
Bud Neck -4.74 -18.45 -16.77 -14.10 -5.76 -8.52 -2.44 -19.97 -15.14 -18.36 -9.30 -20.36 -25.79 -20.51 -8.42
Bud Site -2.42 -0.94 -3.44 -0.51 -3.83 -2.86 -0.53 -4.33 -0.48 -1.92 -3.69 -1.45 -5.37 -0.70 -5.17
Cell Periphery -2.73 -1.14 -1.93 2.02 -1.47 -2.27 3.73 -1.79 0.26 -4.24 -3.23 -1.07 -2.59 1.96 -1.99
Cytoplasm -6.27 10.85 11.36 14.53 2.97 -5.82 9.77 7.97 12.41 -4.12 -8.23 13.47 13.84 19.10 2.04
Cytoplasmic Foci -2.88 3.52 2.88 5.43 -2.39 -3.55 4.78 3.82 6.70 -2.34 -4.67 5.94 4.79 8.61 -3.34
Eisosomes -9.62 -11.90 -11.09 -1.59 -3.70 -1.24 -3.26 -4.04 -3.21 1.55 -7.07 -6.91 -10.43 -3.37 -4.36
Endoplasmic Reticulum -11.66 -11.40 -7.88 3.59 -0.71 -3.95 -2.99 0.61 6.88 3.35 -9.63 -6.20 -4.24 6.70 2.30
Endosome -5.29 1.60 -2.86 0.91 -2.98 -4.82 3.33 -1.20 3.56 -2.69 -6.26 3.33 -2.75 3.65 -3.91
Golgi -4.15 1.37 -1.87 -0.73 -1.98 -2.82 2.23 -1.08 1.99 -2.18 -3.48 2.53 -2.13 1.36 -2.87
Lipid Particles -4.31 4.24 3.86 8.80 -3.68 -2.32 5.24 5.34 5.35 0.51 -3.61 6.82 6.49 7.24 -5.02
Mitochondria -2.95 -0.02 -3.43 0.15 -4.69 -4.20 -0.36 -1.48 3.65 -0.14 -5.20 0.68 -3.53 3.01 -5.86
Mitotic Spindle -1.48 -0.38 -4.30 -3.08 -4.36 -4.89 1.93 -4.75 0.15 -6.08 -5.10 -0.13 -6.40 -1.27 -6.59
None -9.87 3.49 3.61 10.71 0.49 2.35 6.08 5.57 3.46 -1.86 -8.19 6.99 6.65 11.10 -0.67
Nuclear Periphery -1.45 4.08 -2.82 -1.85 -4.72 -4.37 6.08 -3.13 2.15 -5.74 -4.47 2.82 -4.21 0.95 -5.63
Nuclear Periphery Foci -5.48 2.00 -2.64 1.61 -3.53 -0.45 2.35 -0.03 0.45 -2.82 -2.02 2.71 -1.15 1.07 -4.36
Nucleolus -6.35 4.68 -4.81 0.88 -8.46 3.34 5.29 -5.57 -8.21 -9.40 -2.04 8.90 -7.25 -5.94 -13.74
Nucleus 12.71 -2.57 6.57 -7.32 9.85 7.95 -3.67 7.03 -1.83 8.55 14.33 -4.24 9.57 -6.27 14.87
Peroxisomes -2.53 1.77 1.08 3.18 -6.70 -1.41 2.13 2.03 4.83 -0.35 -2.92 3.30 2.31 5.59 -4.61
Vacuole -4.42 -2.55 -5.48 -0.71 -4.73 -7.38 -1.10 -3.29 2.79 -0.09 -8.61 -2.17 -6.10 1.86 -5.16
Vacuole Periphery -6.29 -0.93 -3.57 -2.66 -3.17 -3.50 0.11 -2.80 1.96 -2.53 -4.14 -0.38 -4.43 0.03 -3.84
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YKR011C

YKR011C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr011c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available