Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.95 0.93 0.92 0.84 0.71 0.74 0.63 0.6 0.51 0.57 0.98 1.0 1.0 0.94 0.98 0.98 0.92 0.94 0.78 0.9 0.92 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0.05 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0.06 0.15 0.13 0.38 0.47 0.45 0.6 0.64 0.67 0.64 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 1 0 0 0 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 2 2 0 0 5 6 9 7 7 2 3 2 0 3 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 7 4 7 4 5 4 5 6 3 5 0 4 5 6 3 11 0 0 1 0 0 0
Cytoplasm 171 169 172 126 245 266 403 279 213 219 99 134 97 240 257 192 98 122 179 170 143 101 171 146
Endoplasmic Reticulum 1 3 1 0 3 1 6 1 3 2 2 2 1 2 0 4 7 4 1 0 9 5 2 9
Endosome 0 0 0 0 3 2 5 3 0 0 0 0 0 0 0 1 1 1 1 0 1 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1
Mitochondria 28 5 10 20 35 121 267 170 205 232 130 150 2 0 2 8 4 2 2 4 14 2 5 3
Nucleus 0 0 1 0 0 0 1 1 2 1 0 2 0 0 1 5 1 3 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 2 1 2 3 12 11 3 5 5 4 0 0 1 6 0 0 2 2 8 0 2 3
Unique Cell Count 183 175 181 136 266 318 564 378 340 365 194 235 99 241 258 204 100 125 195 182 183 113 187 171
Labelled Cell Count 203 183 196 151 297 402 707 479 441 475 241 301 103 246 269 222 114 144 195 182 183 113 187 171


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.6 5.8 5.5 5.6 4.8 4.1 4.4 4.2 3.9 4.2 4.1 6.3 6.1 6.3 7.0 7.9 8.9 5.8 6.0 6.9
Std Deviation (1e-4) 0.5 0.7 1.5 1.0 1.9 1.4 1.0 0.8 1.4 0.8 1.2 1.5 0.9 1.0 1.1 1.4 1.9 1.5 1.4 1.5 1.4
Intensity Change (Log2) – – – -0.09 -0.05 -0.28 -0.5 -0.42 -0.47 -0.58 -0.49 -0.52 0.1 0.06 0.1 0.26 0.44 0.61 -0.02 0.03 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.7 0 0 0 0 0 0 0 0 0 0 -0.5 0 1.8
Cytoplasm -0.9 -1.2 -3.7 -6.6 -5.9 -8.0 -8.5 -9.5 -8.7 1.2 3.0 3.2 -0.4 1.2 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 2.6 0 0 0 0 0 0 0 0 0 0 -0.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5983 2.7901 2.4805 2.3514 1.8373 2.314 1.8994 2.9353 2.5248 1.8445 1.9825 2.2989 3.2523 3.7025 3.6718 3.955 3.731 3.7573
Actin 0.0014 0.001 0.0065 0.0005 0.0034 0.0004 0.0287 0.0087 0.0112 0.0007 0.0007 0.0023 0.0361 0.0005 0.0229 0.0005 0.0086 0.0002
Bud 0.0001 0.0003 0.0004 0.0003 0.0053 0.0001 0.0005 0.0007 0.0008 0.0004 0.0001 0.0001 0.0009 0.0009 0.0011 0.0002 0.0001 0.0001
Bud Neck 0.0001 0.0129 0.0007 0.0003 0.0007 0.0009 0.0015 0.0008 0.0004 0.0004 0.0002 0.0003 0.0038 0.0002 0.0014 0.0002 0.0002 0.0003
Bud Periphery 0.0001 0.0002 0.0004 0.0001 0.0516 0.0001 0.0006 0.0005 0.0007 0.0004 0.0001 0.0001 0.0011 0.0007 0.0009 0.0001 0.0001 0
Bud Site 0.0006 0.031 0.0017 0.0003 0.0007 0.0001 0.0059 0.0106 0.0025 0.0011 0.0003 0.0001 0.0046 0.0053 0.0106 0.0001 0.0003 0
Cell Periphery 0.0006 0.0014 0.0011 0.0002 0.0006 0.0002 0.0005 0.0014 0.0004 0.0004 0.0001 0.0001 0.0004 0.0005 0.0004 0.0001 0.0002 0.0001
Cytoplasm 0.5308 0.7484 0.766 0.9177 0.6963 0.8783 0.3914 0.6998 0.6411 0.6403 0.7359 0.752 0.652 0.8281 0.8015 0.8906 0.7827 0.8693
Cytoplasmic Foci 0.0215 0.0081 0.0168 0.0072 0.0241 0.007 0.0828 0.0141 0.0277 0.0138 0.0362 0.0185 0.0374 0.0094 0.011 0.0117 0.0197 0.0102
Eisosomes 0.0002 0.0003 0.0006 0 0.0001 0 0.0005 0.0006 0.0003 0 0 0 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0107 0.0052 0.0096 0.0032 0.0061 0.0142 0.0059 0.0033 0.0066 0.0032 0.0019 0.0043 0.0186 0.0016 0.005 0.0211 0.0027 0.0033
Endosome 0.02 0.0066 0.0144 0.0079 0.0864 0.0089 0.0692 0.011 0.0392 0.0162 0.0276 0.0172 0.0253 0.0122 0.0143 0.0343 0.0329 0.0092
Golgi 0.0042 0.0011 0.0109 0.0004 0.0174 0.0021 0.0276 0.0013 0.0178 0.0005 0.0227 0.0063 0.0107 0.0004 0.004 0.0013 0.0044 0.0009
Lipid Particles 0.0517 0.0004 0.0033 0.0001 0.0199 0.0001 0.0465 0.0004 0.0086 0.0004 0.0055 0.0028 0.0123 0.0001 0.0015 0.001 0.0042 0.0002
Mitochondria 0.0023 0.0011 0.006 0.0002 0.0543 0.0018 0.0079 0.0003 0.015 0.0003 0.0053 0.0062 0.003 0.0001 0.0059 0.0004 0.0016 0.0011
None 0.285 0.178 0.1509 0.0554 0.0037 0.0829 0.2457 0.2399 0.2061 0.3074 0.15 0.1826 0.1564 0.1332 0.11 0.026 0.0916 0.1032
Nuclear Periphery 0.0343 0.0006 0.0009 0.0006 0.0025 0.0004 0.0196 0.0007 0.0017 0.0008 0.0003 0.0003 0.0085 0.0004 0.0008 0.0012 0.0004 0.0002
Nucleolus 0.0003 0.0005 0.0004 0 0.0001 0 0.0017 0.0001 0.0002 0.0002 0.0001 0 0.0008 0.0001 0.0002 0 0 0
Nucleus 0.0134 0.0006 0.0009 0.0008 0.0011 0.0005 0.0097 0.0007 0.0011 0.0009 0.0004 0.0003 0.003 0.0006 0.0007 0.0007 0.0004 0.0003
Peroxisomes 0.0137 0.0008 0.0026 0.0001 0.0011 0.0001 0.0374 0.0005 0.0111 0.0001 0.0097 0.0042 0.013 0 0.0017 0 0.043 0
Punctate Nuclear 0.0025 0.0002 0.0008 0.0002 0.0004 0.0001 0.009 0.0006 0.0008 0.0004 0.0003 0.0002 0.0076 0.0002 0.0004 0.0002 0.0003 0.0001
Vacuole 0.0046 0.0012 0.0046 0.0041 0.0193 0.0015 0.0049 0.0035 0.0057 0.0106 0.0021 0.0014 0.0033 0.0051 0.0046 0.0087 0.0053 0.0012
Vacuole Periphery 0.002 0.0002 0.0005 0.0005 0.0051 0.0003 0.0025 0.0004 0.0011 0.0014 0.0003 0.0006 0.0009 0.0004 0.0006 0.0016 0.001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8486 1.8576 9.1894 10.6101 5.8374 4.8459 8.4519 11.4089 12.9033 7.5492
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1292 936 1776 1223 1620 2220 1196 61 2912 3156 2972 1284

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.79 819.55 1000.46 969.60 770.24 830.39 981.90 930.78 762.94 827.18 992.99 967.76
Standard Deviation 81.91 124.97 115.09 132.05 94.86 108.16 116.82 131.69 89.72 113.51 116.15 132.29
Intensity Change Log 2 — 0.120669 0.408429 0.363227 — 0.108481 0.350268 0.273132 — 0.114522 0.379328 0.318397

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000140 0.000819 0.000106 0.001005 0.000163 0.000404 0.000070 0.004615 0.000153 0.000527 0.000092 0.001176
Bud Neck 0.008604 0.013182 0.001734 0.002183 0.008180 0.014446 0.001375 0.002042 0.008368 0.014071 0.001590 0.002176
Bud Site 0.006923 0.040027 0.005895 0.032176 0.009667 0.027363 0.006184 0.018946 0.008449 0.031119 0.006011 0.031547
Cell Periphery 0.000220 0.000212 0.000059 0.000083 0.000146 0.000117 0.000051 0.000136 0.000179 0.000145 0.000056 0.000085
Cytoplasm 0.542348* 0.493340* 0.723319* 0.688957* 0.548366* 0.507798* 0.697316* 0.404144* 0.545696* 0.503510* 0.712855* 0.675426*
Cytoplasmic Foci 0.272034* 0.267164* 0.044413 0.055277 0.282968* 0.265122* 0.045082 0.108628 0.278117* 0.265727* 0.044682 0.057812
Eisosomes 0.000220 0.000122 0.000016 0.000019 0.000146 0.000097 0.000020 0.000098 0.000179 0.000104 0.000018 0.000022
Endoplasmic Reticulum 0.003215 0.000940 0.001344 0.000359 0.001605 0.001057 0.000614 0.000835 0.002320 0.001022 0.001050 0.000382
Endosome 0.024001 0.045327 0.005573 0.006336 0.023239 0.038576 0.005124 0.018356 0.023577 0.040578 0.005392 0.006907
Golgi 0.008535 0.015333 0.000168 0.002738 0.009591 0.017557 0.000136 0.000353 0.009122 0.016897 0.000155 0.002624
Lipid Particles 0.009061 0.003806 0.000900 0.000635 0.006261 0.003019 0.000851 0.001721 0.007504 0.003252 0.000880 0.000687
Mitochondria 0.001998 0.003652 0.000094 0.001352 0.001844 0.004948 0.000103 0.000090 0.001912 0.004563 0.000098 0.001292
Mitotic Spindle 0.001454 0.000719 0.001514 0.021070 0.000162 0.005747 0.002425 0.001851 0.000735 0.004256 0.001880 0.020157
None 0.002012 0.000691 0.001714 0.002651 0.001245 0.000794 0.002195 0.000216 0.001585 0.000763 0.001908 0.002535
Nuclear Periphery 0.000137 0.000079 0.000566 0.000168 0.000135 0.000047 0.000390 0.000274 0.000136 0.000057 0.000495 0.000173
Nuclear Periphery Foci 0.000414 0.000585 0.002290 0.001938 0.000426 0.000164 0.001950 0.004425 0.000421 0.000289 0.002153 0.002056
Nucleolus 0.000525 0.000264 0.000217 0.000168 0.000486 0.000289 0.000243 0.000136 0.000503 0.000282 0.000228 0.000167
Nucleus 0.029858 0.009164 0.049159 0.025226 0.022336 0.010138 0.051540 0.035324 0.025673 0.009849 0.050117 0.025706
Peroxisomes 0.001953 0.004965 0.000246 0.001214 0.002099 0.003754 0.000197 0.003113 0.002034 0.004113 0.000226 0.001305
Vacuole 0.083038 0.094179 0.160307 0.154428 0.078400 0.092755 0.183712 0.394456* 0.080458 0.093177 0.169726 0.165831
Vacuole Periphery 0.003311 0.005430 0.000365 0.002017 0.002534 0.005809 0.000420 0.000242 0.002879 0.005697 0.000387 0.001933

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.12 0.95 -2.24 0.05 -2.36 -2.08 4.29 -1.01 -0.92 -1.03 -3.59 2.86 -2.43 -1.16 -2.60
Bud Neck -3.18 11.74 12.74 8.39 -0.32 -6.85 12.34 11.32 15.05 -1.27 -7.52 16.41 15.84 17.05 -0.69
Bud Site -13.71 1.19 -5.17 6.20 -5.58 -11.91 3.49 -1.02 1.63 -1.64 -17.90 3.63 -4.77 4.60 -5.89
Cell Periphery 0.26 19.59 14.58 3.39 -1.74 4.03 18.06 1.86 0.08 -4.43 2.57 25.43 13.35 5.11 -2.74
Cytoplasm 4.34 -16.33 -7.78 -10.61 6.64 4.84 -10.42 5.03 3.92 7.78 6.37 -19.49 -7.11 -11.39 6.84
Cytoplasmic Foci 0.75 43.37 41.22 31.42 -1.68 2.83 46.84 14.07 12.85 -4.92 2.60 63.90 56.28 51.99 -3.24
Eisosomes 3.57 24.25 23.90 3.86 -2.08 5.67 19.97 2.99 0.04 -4.94 7.28 31.21 29.68 9.07 -3.36
Endoplasmic Reticulum 4.22 3.41 5.49 5.45 3.09 1.64 3.77 2.83 1.31 -1.35 4.16 4.26 7.10 4.87 3.56
Endosome -7.20 14.24 14.46 15.60 2.27 -8.88 17.77 1.99 5.90 -3.23 -11.35 22.50 19.81 26.72 0.75
Golgi -4.24 13.81 8.25 8.84 -1.60 -8.05 17.46 16.66 20.61 -1.47 -9.33 22.20 10.76 16.48 -1.64
Lipid Particles 4.32 11.43 11.63 3.07 1.35 5.08 9.74 7.15 3.04 -2.75 7.40 14.92 15.05 6.69 0.76
Mitochondria -2.07 6.84 3.05 3.70 -1.44 -4.67 8.09 8.06 7.76 0.62 -5.07 10.55 3.47 6.62 -1.43
Mitotic Spindle 0.95 -0.00 -5.36 -5.84 -5.50 -5.19 -4.05 -1.44 1.14 -0.13 -4.24 -2.54 -5.82 -4.30 -5.32
None 4.00 1.51 -0.00 -3.00 -1.15 1.99 -1.54 5.85 3.80 5.01 4.14 -0.13 -0.79 -2.80 -0.73
Nuclear Periphery 2.92 -2.37 -7.67 -10.51 1.41 3.28 -8.04 -3.60 -5.45 1.39 4.73 -3.56 -7.55 -15.19 1.95
Nuclear Periphery Foci -1.74 -11.63 -7.53 -6.00 2.11 7.07 -5.87 -2.48 -2.60 -1.83 3.63 -12.21 -7.65 -8.27 0.60
Nucleolus 2.50 2.96 3.47 3.18 2.93 1.24 1.61 0.42 -0.31 -0.55 2.29 2.87 3.38 4.26 2.28
Nucleus 15.53 -12.07 -2.74 -13.96 7.52 13.51 -16.53 -1.24 -2.92 3.05 21.03 -20.69 -5.43 -15.20 9.10
Peroxisomes -7.16 7.38 3.82 10.34 -2.43 -5.75 17.68 -0.43 0.65 -1.69 -8.41 15.10 4.78 10.65 -2.94
Vacuole -2.65 -17.68 -20.85 -17.91 -5.67 -4.19 -20.69 -10.73 -10.30 -6.15 -4.77 -27.13 -23.54 -21.42 -5.35
Vacuole Periphery -3.00 8.17 4.33 6.22 -1.35 -9.44 11.98 14.68 18.11 1.12 -8.40 13.56 4.56 9.85 -1.34
Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rqt4

Rqt4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rqt4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available