Standard name | |
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Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 2 | 0 | 0 | 5 | 6 | 9 | 7 | 7 | 2 | 3 | 2 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 7 | 4 | 7 | 4 | 5 | 4 | 5 | 6 | 3 | 5 | 0 | 4 | 5 | 6 | 3 | 11 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 171 | 169 | 172 | 126 | 245 | 266 | 403 | 279 | 213 | 219 | 99 | 134 | 97 | 240 | 257 | 192 | 98 | 122 | 179 | 170 | 143 | 101 | 171 | 146 |
Endoplasmic Reticulum | 1 | 3 | 1 | 0 | 3 | 1 | 6 | 1 | 3 | 2 | 2 | 2 | 1 | 2 | 0 | 4 | 7 | 4 | 1 | 0 | 9 | 5 | 2 | 9 |
Endosome | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 28 | 5 | 10 | 20 | 35 | 121 | 267 | 170 | 205 | 232 | 130 | 150 | 2 | 0 | 2 | 8 | 4 | 2 | 2 | 4 | 14 | 2 | 5 | 3 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 1 | 5 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 2 | 1 | 2 | 3 | 12 | 11 | 3 | 5 | 5 | 4 | 0 | 0 | 1 | 6 | 0 | 0 | 2 | 2 | 8 | 0 | 2 | 3 |
Unique Cell Count | 183 | 175 | 181 | 136 | 266 | 318 | 564 | 378 | 340 | 365 | 194 | 235 | 99 | 241 | 258 | 204 | 100 | 125 | 195 | 182 | 183 | 113 | 187 | 171 |
Labelled Cell Count | 203 | 183 | 196 | 151 | 297 | 402 | 707 | 479 | 441 | 475 | 241 | 301 | 103 | 246 | 269 | 222 | 114 | 144 | 195 | 182 | 183 | 113 | 187 | 171 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.6 | 5.8 | 5.5 | 5.6 | 4.8 | 4.1 | 4.4 | 4.2 | 3.9 | 4.2 | 4.1 | 6.3 | 6.1 | 6.3 | 7.0 | 7.9 | 8.9 | 5.8 | 6.0 | 6.9 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.5 | 1.0 | 1.9 | 1.4 | 1.0 | 0.8 | 1.4 | 0.8 | 1.2 | 1.5 | 0.9 | 1.0 | 1.1 | 1.4 | 1.9 | 1.5 | 1.4 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.05 | -0.28 | -0.5 | -0.42 | -0.47 | -0.58 | -0.49 | -0.52 | 0.1 | 0.06 | 0.1 | 0.26 | 0.44 | 0.61 | -0.02 | 0.03 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.5 | 0 | 1.8 |
Cytoplasm | -0.9 | -1.2 | -3.7 | -6.6 | -5.9 | -8.0 | -8.5 | -9.5 | -8.7 | 1.2 | 3.0 | 3.2 | -0.4 | 1.2 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.8 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5983 | 2.7901 | 2.4805 | 2.3514 | 1.8373 | 2.314 | 1.8994 | 2.9353 | 2.5248 | 1.8445 | 1.9825 | 2.2989 | 3.2523 | 3.7025 | 3.6718 | 3.955 | 3.731 | 3.7573 |
Actin | 0.0014 | 0.001 | 0.0065 | 0.0005 | 0.0034 | 0.0004 | 0.0287 | 0.0087 | 0.0112 | 0.0007 | 0.0007 | 0.0023 | 0.0361 | 0.0005 | 0.0229 | 0.0005 | 0.0086 | 0.0002 |
Bud | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0053 | 0.0001 | 0.0005 | 0.0007 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0009 | 0.0009 | 0.0011 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0001 | 0.0129 | 0.0007 | 0.0003 | 0.0007 | 0.0009 | 0.0015 | 0.0008 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0038 | 0.0002 | 0.0014 | 0.0002 | 0.0002 | 0.0003 |
Bud Periphery | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0516 | 0.0001 | 0.0006 | 0.0005 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0011 | 0.0007 | 0.0009 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0006 | 0.031 | 0.0017 | 0.0003 | 0.0007 | 0.0001 | 0.0059 | 0.0106 | 0.0025 | 0.0011 | 0.0003 | 0.0001 | 0.0046 | 0.0053 | 0.0106 | 0.0001 | 0.0003 | 0 |
Cell Periphery | 0.0006 | 0.0014 | 0.0011 | 0.0002 | 0.0006 | 0.0002 | 0.0005 | 0.0014 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.5308 | 0.7484 | 0.766 | 0.9177 | 0.6963 | 0.8783 | 0.3914 | 0.6998 | 0.6411 | 0.6403 | 0.7359 | 0.752 | 0.652 | 0.8281 | 0.8015 | 0.8906 | 0.7827 | 0.8693 |
Cytoplasmic Foci | 0.0215 | 0.0081 | 0.0168 | 0.0072 | 0.0241 | 0.007 | 0.0828 | 0.0141 | 0.0277 | 0.0138 | 0.0362 | 0.0185 | 0.0374 | 0.0094 | 0.011 | 0.0117 | 0.0197 | 0.0102 |
Eisosomes | 0.0002 | 0.0003 | 0.0006 | 0 | 0.0001 | 0 | 0.0005 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0107 | 0.0052 | 0.0096 | 0.0032 | 0.0061 | 0.0142 | 0.0059 | 0.0033 | 0.0066 | 0.0032 | 0.0019 | 0.0043 | 0.0186 | 0.0016 | 0.005 | 0.0211 | 0.0027 | 0.0033 |
Endosome | 0.02 | 0.0066 | 0.0144 | 0.0079 | 0.0864 | 0.0089 | 0.0692 | 0.011 | 0.0392 | 0.0162 | 0.0276 | 0.0172 | 0.0253 | 0.0122 | 0.0143 | 0.0343 | 0.0329 | 0.0092 |
Golgi | 0.0042 | 0.0011 | 0.0109 | 0.0004 | 0.0174 | 0.0021 | 0.0276 | 0.0013 | 0.0178 | 0.0005 | 0.0227 | 0.0063 | 0.0107 | 0.0004 | 0.004 | 0.0013 | 0.0044 | 0.0009 |
Lipid Particles | 0.0517 | 0.0004 | 0.0033 | 0.0001 | 0.0199 | 0.0001 | 0.0465 | 0.0004 | 0.0086 | 0.0004 | 0.0055 | 0.0028 | 0.0123 | 0.0001 | 0.0015 | 0.001 | 0.0042 | 0.0002 |
Mitochondria | 0.0023 | 0.0011 | 0.006 | 0.0002 | 0.0543 | 0.0018 | 0.0079 | 0.0003 | 0.015 | 0.0003 | 0.0053 | 0.0062 | 0.003 | 0.0001 | 0.0059 | 0.0004 | 0.0016 | 0.0011 |
None | 0.285 | 0.178 | 0.1509 | 0.0554 | 0.0037 | 0.0829 | 0.2457 | 0.2399 | 0.2061 | 0.3074 | 0.15 | 0.1826 | 0.1564 | 0.1332 | 0.11 | 0.026 | 0.0916 | 0.1032 |
Nuclear Periphery | 0.0343 | 0.0006 | 0.0009 | 0.0006 | 0.0025 | 0.0004 | 0.0196 | 0.0007 | 0.0017 | 0.0008 | 0.0003 | 0.0003 | 0.0085 | 0.0004 | 0.0008 | 0.0012 | 0.0004 | 0.0002 |
Nucleolus | 0.0003 | 0.0005 | 0.0004 | 0 | 0.0001 | 0 | 0.0017 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.0134 | 0.0006 | 0.0009 | 0.0008 | 0.0011 | 0.0005 | 0.0097 | 0.0007 | 0.0011 | 0.0009 | 0.0004 | 0.0003 | 0.003 | 0.0006 | 0.0007 | 0.0007 | 0.0004 | 0.0003 |
Peroxisomes | 0.0137 | 0.0008 | 0.0026 | 0.0001 | 0.0011 | 0.0001 | 0.0374 | 0.0005 | 0.0111 | 0.0001 | 0.0097 | 0.0042 | 0.013 | 0 | 0.0017 | 0 | 0.043 | 0 |
Punctate Nuclear | 0.0025 | 0.0002 | 0.0008 | 0.0002 | 0.0004 | 0.0001 | 0.009 | 0.0006 | 0.0008 | 0.0004 | 0.0003 | 0.0002 | 0.0076 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 |
Vacuole | 0.0046 | 0.0012 | 0.0046 | 0.0041 | 0.0193 | 0.0015 | 0.0049 | 0.0035 | 0.0057 | 0.0106 | 0.0021 | 0.0014 | 0.0033 | 0.0051 | 0.0046 | 0.0087 | 0.0053 | 0.0012 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0005 | 0.0005 | 0.0051 | 0.0003 | 0.0025 | 0.0004 | 0.0011 | 0.0014 | 0.0003 | 0.0006 | 0.0009 | 0.0004 | 0.0006 | 0.0016 | 0.001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.8486 | 1.8576 | 9.1894 | 10.6101 | 5.8374 | 4.8459 | 8.4519 | 11.4089 | 12.9033 | 7.5492 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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