Standard name | |
---|---|
Human Ortholog | |
Description | Alpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions; human homolog EIF2B1 can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 1 | 1 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 4 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 2 | 0 | 0 | 5 | 1 | 3 | 8 | 13 | 10 | 8 | 2 | 3 | 8 | 1 | – | 2 | 1 | 0 | 3 | 0 | 0 | 0 |
Cytoplasm | 72 | 36 | 103 | 20 | 40 | 91 | 91 | 95 | 103 | 155 | 85 | 110 | 70 | 118 | 175 | 14 | – | 17 | 87 | 73 | 104 | 73 | 112 | 161 |
Endoplasmic Reticulum | 2 | 1 | 5 | 0 | 2 | 1 | 2 | 1 | 7 | 3 | 2 | 9 | 3 | 9 | 9 | 1 | – | 1 | 5 | 1 | 7 | 9 | 13 | 28 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 3 |
Mitochondria | 25 | 7 | 19 | 8 | 18 | 33 | 79 | 74 | 73 | 99 | 74 | 88 | 1 | 0 | 0 | 0 | – | 2 | 3 | 1 | 7 | 3 | 8 | 7 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 0 | 0 | 0 | 0 | 1 | 9 | 9 | 10 | 3 | 5 | 8 | 0 | 1 | 1 | 0 | – | 1 | 2 | 3 | 3 | 11 | 17 | 13 |
Unique Cell Count | 96 | 40 | 119 | 22 | 49 | 119 | 154 | 152 | 162 | 229 | 142 | 190 | 75 | 128 | 184 | 16 | 19 | 110 | 83 | 130 | 108 | 161 | 225 | |
Labelled Cell Count | 113 | 45 | 130 | 28 | 61 | 135 | 189 | 185 | 206 | 276 | 180 | 235 | 77 | 132 | 195 | 17 | 24 | 110 | 83 | 130 | 108 | 161 | 225 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 8.3 | 8.0 | 8.0 | 6.5 | 6.5 | 6.0 | 5.8 | 5.7 | 5.4 | 5.7 | 5.7 | 10.6 | 10.6 | 11.1 | 13.1 | – | 14.5 | 11.1 | 10.9 | 11.8 |
Std Deviation (1e-4) | 2.1 | 1.4 | 1.6 | 1.4 | 1.0 | 1.8 | 1.7 | 1.4 | 1.2 | 1.2 | 1.2 | 1.1 | 2.1 | 1.8 | 1.5 | 2.5 | – | 3.8 | 1.7 | 2.2 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.31 | -0.3 | -0.43 | -0.46 | -0.5 | -0.56 | -0.5 | -0.49 | 0.4 | 0.4 | 0.46 | 0.7 | – | 0.85 | 0.46 | 0.44 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 2.1 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cytoplasm | 0.6 | -0.8 | -2.0 | -5.0 | -4.4 | -4.3 | -3.8 | -4.8 | -5.3 | 1.5 | 1.4 | 2.6 | 0.1 | – | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 1.0 | 0.3 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Mitochondria | 0 | 3.0 | 2.2 | 6.0 | 5.6 | 5.1 | 5.1 | 6.1 | 5.5 | -3.3 | -4.7 | -5.6 | 0 | – | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.1579 | 9.0268 | 7.8945 | 7.0597 | 6.3599 | 7.6808 | 7.7058 | 10.6795 | 9.4957 | 9.63 | 8.7836 | 9.5733 | 6.0081 | 9.2923 | 7.1088 | 7.0755 | 6.9099 | 7.4027 |
Actin | 0.0112 | 0.0007 | 0.0073 | 0.0002 | 0.0004 | 0.0017 | 0.0159 | 0.0047 | 0.0155 | 0.0006 | 0.0016 | 0.0057 | 0.0075 | 0.0012 | 0.0026 | 0.0047 | 0.0155 | 0.0014 |
Bud | 0.0015 | 0.0007 | 0.0015 | 0.0007 | 0.0011 | 0.0006 | 0.0009 | 0.0014 | 0.0015 | 0.0022 | 0.0015 | 0.0011 | 0.0006 | 0.0017 | 0.0009 | 0.0008 | 0.0008 | 0.0006 |
Bud Neck | 0.0015 | 0.0005 | 0.0007 | 0.0043 | 0.0007 | 0.0017 | 0.0006 | 0.0005 | 0.0009 | 0.0006 | 0.0007 | 0.0017 | 0.0013 | 0.0004 | 0.0014 | 0.0005 | 0.0008 | 0.0011 |
Bud Periphery | 0.0019 | 0.0003 | 0.0011 | 0.0003 | 0.0007 | 0.0006 | 0.0013 | 0.0011 | 0.0013 | 0.0022 | 0.003 | 0.0013 | 0.0007 | 0.001 | 0.001 | 0.0007 | 0.0006 | 0.0007 |
Bud Site | 0.0062 | 0.0108 | 0.0028 | 0.0006 | 0.0017 | 0.0004 | 0.0024 | 0.0071 | 0.0039 | 0.0005 | 0.0006 | 0.0004 | 0.0019 | 0.003 | 0.002 | 0.0004 | 0.0015 | 0.0002 |
Cell Periphery | 0.0015 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.001 | 0.0024 | 0.002 | 0.0006 | 0.0003 | 0.0012 | 0.0007 | 0.0013 | 0.0007 | 0.001 | 0.0003 | 0.0005 | 0.0003 |
Cytoplasm | 0.5786 | 0.8755 | 0.7746 | 0.5765 | 0.57 | 0.6581 | 0.6935 | 0.848 | 0.7135 | 0.5063 | 0.4897 | 0.6923 | 0.6592 | 0.8322 | 0.6784 | 0.5016 | 0.6345 | 0.6998 |
Cytoplasmic Foci | 0.0363 | 0.0156 | 0.0285 | 0.0482 | 0.0484 | 0.03 | 0.0192 | 0.0186 | 0.0361 | 0.0269 | 0.027 | 0.0312 | 0.039 | 0.0237 | 0.0396 | 0.0235 | 0.0255 | 0.0239 |
Eisosomes | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0904 | 0.0213 | 0.0188 | 0.0107 | 0.0179 | 0.0851 | 0.059 | 0.0205 | 0.0255 | 0.0077 | 0.0982 | 0.0658 | 0.0678 | 0.0261 | 0.0255 | 0.0663 | 0.086 | 0.0956 |
Endosome | 0.1123 | 0.0437 | 0.0902 | 0.2286 | 0.2488 | 0.1435 | 0.0768 | 0.0474 | 0.1254 | 0.2543 | 0.2406 | 0.1077 | 0.0926 | 0.0537 | 0.1517 | 0.293 | 0.1605 | 0.1097 |
Golgi | 0.01 | 0.0047 | 0.0066 | 0.0085 | 0.0148 | 0.0233 | 0.0158 | 0.017 | 0.0134 | 0.0307 | 0.016 | 0.0388 | 0.01 | 0.0104 | 0.0136 | 0.0143 | 0.0175 | 0.0201 |
Lipid Particles | 0.0156 | 0.0002 | 0.0021 | 0.0022 | 0.0016 | 0.0005 | 0.0092 | 0.0012 | 0.0023 | 0.0001 | 0.0009 | 0.0009 | 0.0045 | 0.0002 | 0.0056 | 0.0024 | 0.0046 | 0.0005 |
Mitochondria | 0.0086 | 0.0022 | 0.0089 | 0.0017 | 0.0055 | 0.03 | 0.0164 | 0.0136 | 0.0031 | 0.074 | 0.0192 | 0.035 | 0.0036 | 0.0107 | 0.0086 | 0.0071 | 0.0074 | 0.0276 |
None | 0.0421 | 0.0012 | 0.0062 | 0.0028 | 0.0077 | 0.0026 | 0.0412 | 0.0013 | 0.0072 | 0.0006 | 0.0328 | 0.0055 | 0.0388 | 0.0099 | 0.0135 | 0.0072 | 0.0102 | 0.0015 |
Nuclear Periphery | 0.0134 | 0.0013 | 0.0086 | 0.0033 | 0.0046 | 0.0021 | 0.0133 | 0.0009 | 0.0035 | 0.002 | 0.0071 | 0.0014 | 0.0146 | 0.0013 | 0.0071 | 0.0046 | 0.0073 | 0.004 |
Nucleolus | 0.001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0 |
Nucleus | 0.0154 | 0.0012 | 0.0044 | 0.0022 | 0.0026 | 0.0011 | 0.004 | 0.0008 | 0.0019 | 0.001 | 0.0017 | 0.0007 | 0.0057 | 0.0011 | 0.0072 | 0.0013 | 0.0027 | 0.0012 |
Peroxisomes | 0.0018 | 0.0001 | 0.0004 | 0.0009 | 0.0004 | 0.0002 | 0.0012 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0017 | 0.0001 | 0.003 | 0.0004 | 0.0023 | 0.0002 |
Punctate Nuclear | 0.0048 | 0.0002 | 0.001 | 0.0009 | 0.0034 | 0.0001 | 0.0048 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0.0196 | 0.0002 | 0.0058 | 0.0003 | 0.0043 | 0.0008 |
Vacuole | 0.0398 | 0.0166 | 0.0284 | 0.0895 | 0.0564 | 0.0118 | 0.0164 | 0.0116 | 0.0333 | 0.0533 | 0.0316 | 0.0061 | 0.0227 | 0.0188 | 0.024 | 0.0538 | 0.0127 | 0.0069 |
Vacuole Periphery | 0.0056 | 0.0026 | 0.0071 | 0.0176 | 0.0128 | 0.0056 | 0.0037 | 0.0018 | 0.0092 | 0.0364 | 0.0256 | 0.0033 | 0.0061 | 0.0032 | 0.0067 | 0.0161 | 0.0041 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.0786 | 46.2028 | 34.2845 | 49.2416 | 51.4272 | 24.026 | 39.9255 | 35.5628 | 40.3804 | 49.141 |
Translational Efficiency | 1.5788 | 1.1358 | 1.6585 | 0.9456 | 1.0995 | 2.795 | 1.4836 | 1.3805 | 1.217 | 1.2102 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
642 | 1538 | 1605 | 114 | 1773 | 1660 | 2662 | 71 | 2415 | 3198 | 4267 | 185 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 914.58 | 931.18 | 1072.44 | 1149.96 | 863.74 | 969.65 | 1285.91 | 1359.88 | 877.26 | 951.15 | 1205.61 | 1230.52 |
Standard Deviation | 179.99 | 164.86 | 158.49 | 196.88 | 143.27 | 184.53 | 190.32 | 200.86 | 155.52 | 176.39 | 206.73 | 223.14 |
Intensity Change Log 2 | — | 0.025951 | 0.229716 | 0.330402 | — | 0.166867 | 0.574121 | 0.654810 | — | 0.096115 | 0.407263 | 0.497080 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000876 | 0.000374 | 0.000064 | 0.000239 | 0.000245 | 0.000341 | 0.000202 | 0.000373 | 0.000412 | 0.000357 | 0.000150 | 0.000291 |
Bud Neck | 0.002838 | 0.001374 | 0.002428 | 0.011437 | 0.001668 | 0.001027 | 0.000924 | 0.001791 | 0.001979 | 0.001194 | 0.001490 | 0.007735 |
Bud Site | 0.066025 | 0.074113 | 0.005250 | 0.011576 | 0.047289 | 0.048891 | 0.017604 | 0.023184 | 0.052270 | 0.061021 | 0.012957 | 0.016031 |
Cell Periphery | 0.000808 | 0.000559 | 0.000066 | 0.000414 | 0.000378 | 0.000556 | 0.000258 | 0.000162 | 0.000492 | 0.000558 | 0.000186 | 0.000318 |
Cytoplasm | 0.484156* | 0.507990* | 0.594262* | 0.306320* | 0.446924* | 0.513696* | 0.890834* | 0.547569* | 0.456822* | 0.510952* | 0.779281* | 0.398907* |
Cytoplasmic Foci | 0.316484* | 0.246469* | 0.043614 | 0.085343 | 0.297616* | 0.294419* | 0.026866 | 0.162713 | 0.302632* | 0.271359* | 0.033166 | 0.115036 |
Eisosomes | 0.000077 | 0.000021 | 0.000018 | 0.000145 | 0.000031 | 0.000025 | 0.000003 | 0.000010 | 0.000043 | 0.000023 | 0.000009 | 0.000093 |
Endoplasmic Reticulum | 0.007820 | 0.005632 | 0.002984 | 0.000517 | 0.009840 | 0.005334 | 0.000199 | 0.000194 | 0.009303 | 0.005477 | 0.001246 | 0.000393 |
Endosome | 0.021707 | 0.014766 | 0.009072 | 0.032818 | 0.021516 | 0.017072 | 0.003485 | 0.037025 | 0.021567 | 0.015963 | 0.005586 | 0.034432 |
Golgi | 0.029892 | 0.029304 | 0.000282 | 0.004939 | 0.027018 | 0.036363 | 0.006293 | 0.004896 | 0.027782 | 0.032969 | 0.004032 | 0.004922 |
Lipid Particles | 0.002245 | 0.001061 | 0.001453 | 0.001873 | 0.001452 | 0.001831 | 0.000984 | 0.002690 | 0.001663 | 0.001461 | 0.001160 | 0.002187 |
Mitochondria | 0.008713 | 0.016042 | 0.000220 | 0.013170 | 0.021238 | 0.010537 | 0.000878 | 0.015685 | 0.017908 | 0.013185 | 0.000630 | 0.014135 |
Mitotic Spindle | 0.005135 | 0.004533 | 0.002519 | 0.006769 | 0.003947 | 0.003882 | 0.002326 | 0.017720 | 0.004263 | 0.004195 | 0.002399 | 0.010971 |
None | 0.000399 | 0.001011 | 0.001558 | 0.002959 | 0.000728 | 0.001678 | 0.000298 | 0.000133 | 0.000641 | 0.001357 | 0.000772 | 0.001875 |
Nuclear Periphery | 0.000154 | 0.000074 | 0.000769 | 0.000319 | 0.000075 | 0.000165 | 0.000011 | 0.000551 | 0.000096 | 0.000121 | 0.000296 | 0.000408 |
Nuclear Periphery Foci | 0.001477 | 0.000313 | 0.001124 | 0.001461 | 0.000408 | 0.000555 | 0.000289 | 0.001453 | 0.000692 | 0.000438 | 0.000603 | 0.001458 |
Nucleolus | 0.000135 | 0.000541 | 0.000442 | 0.000284 | 0.000186 | 0.000997 | 0.000100 | 0.000091 | 0.000173 | 0.000777 | 0.000229 | 0.000210 |
Nucleus | 0.002005 | 0.003553 | 0.049370 | 0.053625 | 0.002347 | 0.002054 | 0.002511 | 0.003679 | 0.002256 | 0.002775 | 0.020137 | 0.034456 |
Peroxisomes | 0.001210 | 0.001042 | 0.000359 | 0.002417 | 0.000774 | 0.000928 | 0.000730 | 0.004374 | 0.000890 | 0.000983 | 0.000590 | 0.003168 |
Vacuole | 0.022834 | 0.029141 | 0.282598* | 0.453366* | 0.035426 | 0.020770 | 0.040554 | 0.165859 | 0.032079 | 0.024796 | 0.131597 | 0.343025* |
Vacuole Periphery | 0.025009 | 0.062087 | 0.001549 | 0.010011 | 0.080894 | 0.038877 | 0.004652 | 0.009848 | 0.066038 | 0.050040 | 0.003485 | 0.009948 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.50 | 2.42 | 1.10 | -0.23 | -1.54 | -2.31 | 0.83 | -1.26 | -0.15 | -1.57 | 0.58 | 2.75 | 0.07 | -0.28 | -1.65 |
Bud Neck | 5.19 | 1.22 | -1.72 | -2.22 | -1.86 | 4.59 | 4.91 | -1.75 | -3.22 | -3.42 | 6.01 | 3.21 | -2.09 | -2.52 | -2.36 |
Bud Site | -1.80 | 16.04 | 11.63 | 17.21* | -4.54 | -0.67 | 16.95* | 4.32 | 4.56 | -1.35 | -4.02 | 24.22 | 11.79 | 14.75 | -2.50 |
Cell Periphery | 1.33 | 4.16 | 2.54 | 2.32 | -1.62 | -1.14 | 4.85 | 4.79 | 2.66 | 2.51 | -0.68 | 6.09 | 3.12 | 3.09 | -0.31 |
Cytoplasm | -1.79 | -8.79 | 5.85 | 6.91 | 10.19 | -6.55 | -59.51* | -1.54 | 0.19 | 10.08 | -6.88 | -46.06 | 2.91 | 5.19 | 16.73 |
Cytoplasmic Foci | 7.11 | 32.53 | 15.77 | 12.17 | -3.88 | 0.47 | 53.76 | 7.20 | 6.97 | -5.95 | 5.49 | 62.43 | 16.46 | 13.90 | -7.22 |
Eisosomes | 4.39 | 4.76 | -0.22 | -1.27 | -1.36 | 1.52 | 15.26 | 8.85 | 4.46 | -4.56 | 4.88 | 9.53 | -0.48 | -1.07 | -1.53 |
Endoplasmic Reticulum | 1.64 | 3.91 | 5.85 | 11.35 | 6.31 | 3.80 | 9.38 | 9.42 | 8.80 | 0.88 | 4.22 | 9.76 | 10.85 | 13.63 | 5.60 |
Endosome | 4.40 | 8.72 | -1.07 | -2.67 | -4.08 | 4.43 | 22.80 | -0.56 | -1.12 | -2.85 | 7.00 | 23.05 | -1.18 | -2.55 | -5.18 |
Golgi | 0.26 | 17.71 | 10.99 | 12.32 | -1.46 | -5.99 | 20.02 | 13.26 | 15.93 | 1.87 | -4.46 | 28.05 | 16.92 | 19.39 | 0.79 |
Lipid Particles | 2.99 | 2.36 | -0.49 | -3.40 | -2.82 | -0.88 | 2.29 | -2.36 | -1.64 | -2.90 | 0.58 | 3.01 | -2.83 | -2.91 | -4.24 |
Mitochondria | -2.91 | 4.18 | 0.26 | 1.74 | -1.51 | 5.36 | 12.73 | 1.56 | 0.24 | -0.97 | 2.93 | 13.35 | 2.28 | 1.24 | -1.71 |
Mitotic Spindle | 0.28 | 1.22 | -2.19 | -2.29 | -2.50 | -0.01 | 1.47 | -2.52 | -2.52 | -2.64 | 0.04 | 1.91 | -3.38 | -3.39 | -3.62 |
None | -1.81 | -2.87 | -0.88 | -0.47 | -0.18 | -1.36 | 2.68 | 5.26 | 2.23 | 1.28 | -1.81 | -0.53 | -0.51 | 0.25 | -0.41 |
Nuclear Periphery | 0.96 | -2.16 | -1.92 | -2.91 | 1.07 | -0.99 | 5.16 | -1.28 | -0.87 | -1.53 | -0.49 | -1.90 | -2.63 | -2.27 | -0.90 |
Nuclear Periphery Foci | 1.21 | 0.34 | -0.81 | -4.57 | -2.64 | -1.04 | 0.55 | -3.72 | -3.19 | -4.09 | 0.78 | 0.19 | -3.62 | -5.39 | -4.96 |
Nucleolus | -1.54 | -2.15 | -0.73 | 1.31 | 2.49 | -1.96 | 1.18 | 1.02 | 2.14 | -0.58 | -2.42 | -0.61 | -0.27 | 2.41 | 0.63 |
Nucleus | -1.60 | -24.60 | -3.16 | -3.00 | 1.41 | 0.37 | -0.68 | -2.11 | -2.36 | -1.99 | -1.07 | -21.24 | -3.20 | -3.11 | -0.42 |
Peroxisomes | 0.78 | 9.08 | -3.08 | -3.23 | -5.03 | -1.38 | 0.46 | -2.90 | -2.71 | -2.94 | -0.97 | 3.77 | -4.40 | -4.11 | -5.00 |
Vacuole | -2.57 | -35.78 | -15.04 | -14.83 | -6.06 | 7.14 | -2.83 | -6.70 | -7.22 | -6.48 | 4.42 | -28.09 | -14.98 | -15.32 | -10.50 |
Vacuole Periphery | -10.07 | 10.59 | 5.90 | 14.89 | -1.84 | 11.84 | 25.16 | 14.81 | 7.37 | -0.23 | 5.50 | 26.62 | 19.02 | 16.07 | -1.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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