Standard name
Human Ortholog
Description Alpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions; human homolog EIF2B1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0.06 – 0.11 0 0 0 0 0 0
Cytoplasm 0.75 0.9 0.87 0.91 0.82 0.76 0.59 0.62 0.64 0.68 0.6 0.58 0.93 0.92 0.95 0.88 – 0.89 0.79 0.89 0.81 0.68 0.7 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.05 0.06 – 0.05 0.05 0 0.06 0.09 0.09 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.26 0.17 0.16 0.36 0.37 0.28 0.51 0.49 0.45 0.43 0.52 0.46 0 0 0 0 – 0.11 0 0 0.06 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0 0 – 0.05 0 0 0 0.1 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 0 0 0 0 0 0 0 0 1 0 4 1 1 1 1 – 1 1 0 0 0 0 0
Bud 0 0 0 0 0 3 1 3 4 0 1 3 0 0 0 0 – 0 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 2 0 2 0 0 5 1 3 8 13 10 8 2 3 8 1 – 2 1 0 3 0 0 0
Cytoplasm 72 36 103 20 40 91 91 95 103 155 85 110 70 118 175 14 – 17 87 73 104 73 112 161
Endoplasmic Reticulum 2 1 5 0 2 1 2 1 7 3 2 9 3 9 9 1 – 1 5 1 7 9 13 28
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 1 1
Golgi 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 2 3
Mitochondria 25 7 19 8 18 33 79 74 73 99 74 88 1 0 0 0 – 2 3 1 7 3 8 7
Nucleus 0 1 0 0 1 1 0 0 0 1 0 3 0 0 0 0 – 0 1 0 0 1 0 0
Nuclear Periphery 0 0 1 0 0 0 4 0 0 0 3 1 0 0 0 0 – 0 0 0 1 0 0 0
Nucleolus 1 0 0 0 0 0 2 0 0 1 0 1 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 0 0 0 1 9 9 10 3 5 8 0 1 1 0 – 1 2 3 3 11 17 13
Unique Cell Count 96 40 119 22 49 119 154 152 162 229 142 190 75 128 184 16 19 110 83 130 108 161 225
Labelled Cell Count 113 45 130 28 61 135 189 185 206 276 180 235 77 132 195 17 24 110 83 130 108 161 225


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 8.3 8.0 8.0 6.5 6.5 6.0 5.8 5.7 5.4 5.7 5.7 10.6 10.6 11.1 13.1 – 14.5 11.1 10.9 11.8
Std Deviation (1e-4) 2.1 1.4 1.6 1.4 1.0 1.8 1.7 1.4 1.2 1.2 1.2 1.1 2.1 1.8 1.5 2.5 – 3.8 1.7 2.2 1.9
Intensity Change (Log2) – – – -0.01 -0.31 -0.3 -0.43 -0.46 -0.5 -0.56 -0.5 -0.49 0.4 0.4 0.46 0.7 – 0.85 0.46 0.44 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 1.7 2.1 0 0 0 0 0 – 0
Cytoplasm 0.6 -0.8 -2.0 -5.0 -4.4 -4.3 -3.8 -4.8 -5.3 1.5 1.4 2.6 0.1 – 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.2 0 1.0 0.3 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 0 3.0 2.2 6.0 5.6 5.1 5.1 6.1 5.5 -3.3 -4.7 -5.6 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1579 9.0268 7.8945 7.0597 6.3599 7.6808 7.7058 10.6795 9.4957 9.63 8.7836 9.5733 6.0081 9.2923 7.1088 7.0755 6.9099 7.4027
Actin 0.0112 0.0007 0.0073 0.0002 0.0004 0.0017 0.0159 0.0047 0.0155 0.0006 0.0016 0.0057 0.0075 0.0012 0.0026 0.0047 0.0155 0.0014
Bud 0.0015 0.0007 0.0015 0.0007 0.0011 0.0006 0.0009 0.0014 0.0015 0.0022 0.0015 0.0011 0.0006 0.0017 0.0009 0.0008 0.0008 0.0006
Bud Neck 0.0015 0.0005 0.0007 0.0043 0.0007 0.0017 0.0006 0.0005 0.0009 0.0006 0.0007 0.0017 0.0013 0.0004 0.0014 0.0005 0.0008 0.0011
Bud Periphery 0.0019 0.0003 0.0011 0.0003 0.0007 0.0006 0.0013 0.0011 0.0013 0.0022 0.003 0.0013 0.0007 0.001 0.001 0.0007 0.0006 0.0007
Bud Site 0.0062 0.0108 0.0028 0.0006 0.0017 0.0004 0.0024 0.0071 0.0039 0.0005 0.0006 0.0004 0.0019 0.003 0.002 0.0004 0.0015 0.0002
Cell Periphery 0.0015 0.0004 0.0006 0.0002 0.0002 0.001 0.0024 0.002 0.0006 0.0003 0.0012 0.0007 0.0013 0.0007 0.001 0.0003 0.0005 0.0003
Cytoplasm 0.5786 0.8755 0.7746 0.5765 0.57 0.6581 0.6935 0.848 0.7135 0.5063 0.4897 0.6923 0.6592 0.8322 0.6784 0.5016 0.6345 0.6998
Cytoplasmic Foci 0.0363 0.0156 0.0285 0.0482 0.0484 0.03 0.0192 0.0186 0.0361 0.0269 0.027 0.0312 0.039 0.0237 0.0396 0.0235 0.0255 0.0239
Eisosomes 0.0006 0 0 0 0 0 0.0006 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0.0005 0.0002 0
Endoplasmic Reticulum 0.0904 0.0213 0.0188 0.0107 0.0179 0.0851 0.059 0.0205 0.0255 0.0077 0.0982 0.0658 0.0678 0.0261 0.0255 0.0663 0.086 0.0956
Endosome 0.1123 0.0437 0.0902 0.2286 0.2488 0.1435 0.0768 0.0474 0.1254 0.2543 0.2406 0.1077 0.0926 0.0537 0.1517 0.293 0.1605 0.1097
Golgi 0.01 0.0047 0.0066 0.0085 0.0148 0.0233 0.0158 0.017 0.0134 0.0307 0.016 0.0388 0.01 0.0104 0.0136 0.0143 0.0175 0.0201
Lipid Particles 0.0156 0.0002 0.0021 0.0022 0.0016 0.0005 0.0092 0.0012 0.0023 0.0001 0.0009 0.0009 0.0045 0.0002 0.0056 0.0024 0.0046 0.0005
Mitochondria 0.0086 0.0022 0.0089 0.0017 0.0055 0.03 0.0164 0.0136 0.0031 0.074 0.0192 0.035 0.0036 0.0107 0.0086 0.0071 0.0074 0.0276
None 0.0421 0.0012 0.0062 0.0028 0.0077 0.0026 0.0412 0.0013 0.0072 0.0006 0.0328 0.0055 0.0388 0.0099 0.0135 0.0072 0.0102 0.0015
Nuclear Periphery 0.0134 0.0013 0.0086 0.0033 0.0046 0.0021 0.0133 0.0009 0.0035 0.002 0.0071 0.0014 0.0146 0.0013 0.0071 0.0046 0.0073 0.004
Nucleolus 0.001 0 0.0002 0.0001 0.0002 0 0.0012 0 0.0001 0 0.0001 0 0.0006 0.0002 0.0006 0.0001 0.0003 0
Nucleus 0.0154 0.0012 0.0044 0.0022 0.0026 0.0011 0.004 0.0008 0.0019 0.001 0.0017 0.0007 0.0057 0.0011 0.0072 0.0013 0.0027 0.0012
Peroxisomes 0.0018 0.0001 0.0004 0.0009 0.0004 0.0002 0.0012 0.0001 0.0008 0.0002 0.0002 0.0003 0.0017 0.0001 0.003 0.0004 0.0023 0.0002
Punctate Nuclear 0.0048 0.0002 0.001 0.0009 0.0034 0.0001 0.0048 0.0001 0.0009 0.0001 0.0005 0.0001 0.0196 0.0002 0.0058 0.0003 0.0043 0.0008
Vacuole 0.0398 0.0166 0.0284 0.0895 0.0564 0.0118 0.0164 0.0116 0.0333 0.0533 0.0316 0.0061 0.0227 0.0188 0.024 0.0538 0.0127 0.0069
Vacuole Periphery 0.0056 0.0026 0.0071 0.0176 0.0128 0.0056 0.0037 0.0018 0.0092 0.0364 0.0256 0.0033 0.0061 0.0032 0.0067 0.0161 0.0041 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.0786 46.2028 34.2845 49.2416 51.4272 24.026 39.9255 35.5628 40.3804 49.141
Translational Efficiency 1.5788 1.1358 1.6585 0.9456 1.0995 2.795 1.4836 1.3805 1.217 1.2102

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
642 1538 1605 114 1773 1660 2662 71 2415 3198 4267 185

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 914.58 931.18 1072.44 1149.96 863.74 969.65 1285.91 1359.88 877.26 951.15 1205.61 1230.52
Standard Deviation 179.99 164.86 158.49 196.88 143.27 184.53 190.32 200.86 155.52 176.39 206.73 223.14
Intensity Change Log 2 — 0.025951 0.229716 0.330402 — 0.166867 0.574121 0.654810 — 0.096115 0.407263 0.497080

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000876 0.000374 0.000064 0.000239 0.000245 0.000341 0.000202 0.000373 0.000412 0.000357 0.000150 0.000291
Bud Neck 0.002838 0.001374 0.002428 0.011437 0.001668 0.001027 0.000924 0.001791 0.001979 0.001194 0.001490 0.007735
Bud Site 0.066025 0.074113 0.005250 0.011576 0.047289 0.048891 0.017604 0.023184 0.052270 0.061021 0.012957 0.016031
Cell Periphery 0.000808 0.000559 0.000066 0.000414 0.000378 0.000556 0.000258 0.000162 0.000492 0.000558 0.000186 0.000318
Cytoplasm 0.484156* 0.507990* 0.594262* 0.306320* 0.446924* 0.513696* 0.890834* 0.547569* 0.456822* 0.510952* 0.779281* 0.398907*
Cytoplasmic Foci 0.316484* 0.246469* 0.043614 0.085343 0.297616* 0.294419* 0.026866 0.162713 0.302632* 0.271359* 0.033166 0.115036
Eisosomes 0.000077 0.000021 0.000018 0.000145 0.000031 0.000025 0.000003 0.000010 0.000043 0.000023 0.000009 0.000093
Endoplasmic Reticulum 0.007820 0.005632 0.002984 0.000517 0.009840 0.005334 0.000199 0.000194 0.009303 0.005477 0.001246 0.000393
Endosome 0.021707 0.014766 0.009072 0.032818 0.021516 0.017072 0.003485 0.037025 0.021567 0.015963 0.005586 0.034432
Golgi 0.029892 0.029304 0.000282 0.004939 0.027018 0.036363 0.006293 0.004896 0.027782 0.032969 0.004032 0.004922
Lipid Particles 0.002245 0.001061 0.001453 0.001873 0.001452 0.001831 0.000984 0.002690 0.001663 0.001461 0.001160 0.002187
Mitochondria 0.008713 0.016042 0.000220 0.013170 0.021238 0.010537 0.000878 0.015685 0.017908 0.013185 0.000630 0.014135
Mitotic Spindle 0.005135 0.004533 0.002519 0.006769 0.003947 0.003882 0.002326 0.017720 0.004263 0.004195 0.002399 0.010971
None 0.000399 0.001011 0.001558 0.002959 0.000728 0.001678 0.000298 0.000133 0.000641 0.001357 0.000772 0.001875
Nuclear Periphery 0.000154 0.000074 0.000769 0.000319 0.000075 0.000165 0.000011 0.000551 0.000096 0.000121 0.000296 0.000408
Nuclear Periphery Foci 0.001477 0.000313 0.001124 0.001461 0.000408 0.000555 0.000289 0.001453 0.000692 0.000438 0.000603 0.001458
Nucleolus 0.000135 0.000541 0.000442 0.000284 0.000186 0.000997 0.000100 0.000091 0.000173 0.000777 0.000229 0.000210
Nucleus 0.002005 0.003553 0.049370 0.053625 0.002347 0.002054 0.002511 0.003679 0.002256 0.002775 0.020137 0.034456
Peroxisomes 0.001210 0.001042 0.000359 0.002417 0.000774 0.000928 0.000730 0.004374 0.000890 0.000983 0.000590 0.003168
Vacuole 0.022834 0.029141 0.282598* 0.453366* 0.035426 0.020770 0.040554 0.165859 0.032079 0.024796 0.131597 0.343025*
Vacuole Periphery 0.025009 0.062087 0.001549 0.010011 0.080894 0.038877 0.004652 0.009848 0.066038 0.050040 0.003485 0.009948

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.50 2.42 1.10 -0.23 -1.54 -2.31 0.83 -1.26 -0.15 -1.57 0.58 2.75 0.07 -0.28 -1.65
Bud Neck 5.19 1.22 -1.72 -2.22 -1.86 4.59 4.91 -1.75 -3.22 -3.42 6.01 3.21 -2.09 -2.52 -2.36
Bud Site -1.80 16.04 11.63 17.21* -4.54 -0.67 16.95* 4.32 4.56 -1.35 -4.02 24.22 11.79 14.75 -2.50
Cell Periphery 1.33 4.16 2.54 2.32 -1.62 -1.14 4.85 4.79 2.66 2.51 -0.68 6.09 3.12 3.09 -0.31
Cytoplasm -1.79 -8.79 5.85 6.91 10.19 -6.55 -59.51* -1.54 0.19 10.08 -6.88 -46.06 2.91 5.19 16.73
Cytoplasmic Foci 7.11 32.53 15.77 12.17 -3.88 0.47 53.76 7.20 6.97 -5.95 5.49 62.43 16.46 13.90 -7.22
Eisosomes 4.39 4.76 -0.22 -1.27 -1.36 1.52 15.26 8.85 4.46 -4.56 4.88 9.53 -0.48 -1.07 -1.53
Endoplasmic Reticulum 1.64 3.91 5.85 11.35 6.31 3.80 9.38 9.42 8.80 0.88 4.22 9.76 10.85 13.63 5.60
Endosome 4.40 8.72 -1.07 -2.67 -4.08 4.43 22.80 -0.56 -1.12 -2.85 7.00 23.05 -1.18 -2.55 -5.18
Golgi 0.26 17.71 10.99 12.32 -1.46 -5.99 20.02 13.26 15.93 1.87 -4.46 28.05 16.92 19.39 0.79
Lipid Particles 2.99 2.36 -0.49 -3.40 -2.82 -0.88 2.29 -2.36 -1.64 -2.90 0.58 3.01 -2.83 -2.91 -4.24
Mitochondria -2.91 4.18 0.26 1.74 -1.51 5.36 12.73 1.56 0.24 -0.97 2.93 13.35 2.28 1.24 -1.71
Mitotic Spindle 0.28 1.22 -2.19 -2.29 -2.50 -0.01 1.47 -2.52 -2.52 -2.64 0.04 1.91 -3.38 -3.39 -3.62
None -1.81 -2.87 -0.88 -0.47 -0.18 -1.36 2.68 5.26 2.23 1.28 -1.81 -0.53 -0.51 0.25 -0.41
Nuclear Periphery 0.96 -2.16 -1.92 -2.91 1.07 -0.99 5.16 -1.28 -0.87 -1.53 -0.49 -1.90 -2.63 -2.27 -0.90
Nuclear Periphery Foci 1.21 0.34 -0.81 -4.57 -2.64 -1.04 0.55 -3.72 -3.19 -4.09 0.78 0.19 -3.62 -5.39 -4.96
Nucleolus -1.54 -2.15 -0.73 1.31 2.49 -1.96 1.18 1.02 2.14 -0.58 -2.42 -0.61 -0.27 2.41 0.63
Nucleus -1.60 -24.60 -3.16 -3.00 1.41 0.37 -0.68 -2.11 -2.36 -1.99 -1.07 -21.24 -3.20 -3.11 -0.42
Peroxisomes 0.78 9.08 -3.08 -3.23 -5.03 -1.38 0.46 -2.90 -2.71 -2.94 -0.97 3.77 -4.40 -4.11 -5.00
Vacuole -2.57 -35.78 -15.04 -14.83 -6.06 7.14 -2.83 -6.70 -7.22 -6.48 4.42 -28.09 -14.98 -15.32 -10.50
Vacuole Periphery -10.07 10.59 5.90 14.89 -1.84 11.84 25.16 14.81 7.37 -0.23 5.50 26.62 19.02 16.07 -1.06
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Alpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions; human homolog EIF2B1 can complement yeast null mutant
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Gcn3

Gcn3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcn3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available