Standard name | |
---|---|
Human Ortholog | |
Description | Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 17 | 14 | 20 | 33 | 39 | 38 | 22 | 12 | – | 22 | 13 | 15 | 17 | 18 | 13 | 12 | 20 | 46 | 32 | 40 | 25 | 31 | 51 |
Bud | 8 | 20 | 16 | 15 | 31 | 38 | 50 | 44 | 33 | – | 30 | 28 | 8 | 8 | 7 | 4 | 3 | 1 | 1 | 3 | 2 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 17 | 31 | 22 | 15 | 19 | 25 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 124 | 92 | 84 | 99 | 83 | 74 | 93 | 58 | 62 | – | 50 | 41 | 61 | 95 | 116 | 27 | 25 | 19 | 149 | 128 | 128 | 106 | 118 | 115 |
Cytoplasm | 230 | 255 | 244 | 166 | 268 | 209 | 282 | 362 | 227 | – | 162 | 196 | 198 | 172 | 189 | 68 | 55 | 61 | 77 | 102 | 56 | 41 | 73 | 49 |
Endoplasmic Reticulum | 7 | 4 | 1 | 1 | 3 | 4 | 5 | 1 | 3 | – | 2 | 0 | 5 | 1 | 3 | 10 | 3 | 9 | 17 | 13 | 15 | 3 | 10 | 23 |
Endosome | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | – | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 1 | 1 | 2 | 6 |
Golgi | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 2 | 3 | 0 | 1 | 1 |
Mitochondria | 2 | 4 | 10 | 27 | 34 | 81 | 114 | 124 | 128 | – | 113 | 118 | 3 | 3 | 2 | 5 | 3 | 5 | 5 | 17 | 26 | 6 | 17 | 22 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | – | 1 | 1 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 1 | 6 | 4 | 19 | 5 | 2 | – | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 0 | 7 | 1 | 4 | 11 | 3 | – | 4 | 5 | 3 | 0 | 1 | 3 | 1 | 7 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 2 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 9 | 6 | 2 | 5 | 8 |
Unique Cell Count | 292 | 310 | 284 | 213 | 360 | 334 | 444 | 506 | 352 | 287 | 302 | 236 | 218 | 244 | 106 | 88 | 104 | 326 | 356 | 309 | 209 | 286 | 315 | |
Labelled Cell Count | 387 | 396 | 372 | 330 | 473 | 450 | 605 | 633 | 478 | 391 | 405 | 295 | 299 | 342 | 137 | 107 | 130 | 326 | 356 | 309 | 209 | 286 | 315 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 5.7 | 5.2 | 5.2 | 5.2 | 4.7 | 4.4 | 4.3 | 4.0 | – | 3.9 | 3.9 | 5.9 | 5.8 | 5.3 | 7.0 | 7.3 | 7.4 | 6.3 | 6.0 | 6.2 |
Std Deviation (1e-4) | 0.8 | 0.8 | 0.9 | 1.5 | 1.5 | 1.5 | 1.3 | 1.4 | 1.3 | – | 1.5 | 1.2 | 1.0 | 1.0 | 1.0 | 1.6 | 2.1 | 2.0 | 1.0 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.01 | -0.16 | -0.26 | -0.27 | -0.38 | – | -0.4 | -0.43 | 0.17 | 0.16 | 0.03 | 0.42 | 0.5 | 0.5 | 0.27 | 0.21 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.9 | 2.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.7 | 1.3 | 1.2 | 2.5 | 2.8 | 4.4 |
Bud | 0.6 | 1.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.2 | -1.0 | -1.6 | -0.7 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.9 | -1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.9 | 3.2 | 4.2 | -0.8 | -0.2 | -2.2 |
Cytoplasm | -2.3 | -3.6 | -6.5 | -6.6 | -4.6 | -6.1 | – | -7.8 | -5.9 | -0.6 | -2.1 | -2.5 | -4.8 | -4.8 | -5.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.8 | 3.0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.6 | -1.5 | -2.1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.074 | 0.015 | 0.0379 | 0.019 | 0.0605 | 0.0151 | 0.0679 | 0.0071 | 0.02 | 0.1004 | 0.0068 | 0.0364 | 0.0003 | 0.0463 | 0.0121 | 0.0292 | 0.0128 | 0.0628 |
Bud | 0.0008 | 0.0003 | 0.0015 | 0.0006 | 0.001 | 0.0008 | 0.0005 | 0.0093 | 0.0005 | 0.0011 | 0.0005 | 0.0056 | 0 | 0.0015 | 0.0002 | 0.0011 | 0.0002 | 0.0017 |
Bud Neck | 0.0057 | 0.0024 | 0.0118 | 0.0113 | 0.039 | 0.0264 | 0.0038 | 0.0052 | 0.0081 | 0.0375 | 0.0044 | 0.0269 | 0.0005 | 0.0061 | 0.0075 | 0.0055 | 0.0123 | 0.0098 |
Bud Periphery | 0.0033 | 0.0016 | 0.0089 | 0.0041 | 0.0044 | 0.0059 | 0.0023 | 0.0042 | 0.0034 | 0.0042 | 0.004 | 0.0115 | 0 | 0.0034 | 0.0027 | 0.0066 | 0.0013 | 0.0116 |
Bud Site | 0.0136 | 0.0353 | 0.0152 | 0.0049 | 0.0108 | 0.0026 | 0.0096 | 0.0849 | 0.0146 | 0.0295 | 0.0032 | 0.0028 | 0.0002 | 0.1058 | 0.0068 | 0.0074 | 0.001 | 0.0022 |
Cell Periphery | 0.1529 | 0.1649 | 0.1436 | 0.1563 | 0.1065 | 0.0888 | 0.145 | 0.1726 | 0.1552 | 0.1801 | 0.1642 | 0.1734 | 0.0109 | 0.0772 | 0.2109 | 0.2015 | 0.0855 | 0.2125 |
Cytoplasm | 0.5541 | 0.7596 | 0.708 | 0.7272 | 0.7302 | 0.8254 | 0.4841 | 0.6753 | 0.7183 | 0.4433 | 0.5669 | 0.7124 | 0.9807 | 0.6409 | 0.7399 | 0.6539 | 0.8704 | 0.6491 |
Cytoplasmic Foci | 0.0183 | 0.0006 | 0.0018 | 0.0064 | 0.0057 | 0.0014 | 0.0309 | 0.0008 | 0.0053 | 0.026 | 0.0194 | 0.0009 | 0.0003 | 0.0065 | 0.0011 | 0.0052 | 0.0023 | 0.0015 |
Eisosomes | 0.0128 | 0.0005 | 0.0028 | 0.0022 | 0.0009 | 0.0005 | 0.0058 | 0.0019 | 0.0013 | 0.0007 | 0.0003 | 0.0005 | 0 | 0.0091 | 0.0018 | 0.0008 | 0.0003 | 0.0007 |
Endoplasmic Reticulum | 0.0065 | 0.002 | 0.0061 | 0.0039 | 0.005 | 0.0033 | 0.0062 | 0.0018 | 0.002 | 0.0029 | 0.0018 | 0.0026 | 0.0047 | 0.0052 | 0.0037 | 0.0042 | 0.0019 | 0.0024 |
Endosome | 0.0054 | 0 | 0.0019 | 0.0058 | 0.0053 | 0.0003 | 0.0078 | 0.0001 | 0.0013 | 0.0185 | 0.0319 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0203 | 0.0001 | 0.0002 |
Golgi | 0.0055 | 0.0002 | 0.0028 | 0.0041 | 0.0067 | 0.0006 | 0.0045 | 0.0004 | 0.0046 | 0.0328 | 0.0488 | 0.0005 | 0.0001 | 0.0028 | 0.0005 | 0.0071 | 0.0006 | 0.0007 |
Lipid Particles | 0.0069 | 0.0005 | 0.0032 | 0.0032 | 0.0033 | 0.0008 | 0.0082 | 0.0004 | 0.0018 | 0.0282 | 0.0063 | 0.0004 | 0.0002 | 0.0016 | 0.0005 | 0.0148 | 0.0002 | 0.0009 |
Mitochondria | 0.0009 | 0 | 0.0156 | 0.0018 | 0.0022 | 0.0001 | 0.0032 | 0.0008 | 0.0079 | 0.0098 | 0.0073 | 0 | 0 | 0.0001 | 0 | 0.0063 | 0 | 0.0002 |
None | 0.1164 | 0.0159 | 0.0317 | 0.0402 | 0.0096 | 0.0249 | 0.1532 | 0.0337 | 0.0458 | 0.0479 | 0.1282 | 0.0249 | 0.0017 | 0.0909 | 0.0115 | 0.0232 | 0.0106 | 0.0424 |
Nuclear Periphery | 0.0014 | 0.0001 | 0.0033 | 0.0005 | 0.0011 | 0.0001 | 0.031 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0037 | 0.0001 | 0.0002 |
Nucleolus | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 |
Nucleus | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0012 | 0.0004 | 0.0052 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0056 | 0 | 0.0009 | 0.0055 | 0.0019 | 0 | 0.021 | 0.0001 | 0.0085 | 0.0326 | 0.0039 | 0 | 0 | 0.0004 | 0 | 0.0034 | 0 | 0.0001 |
Punctate Nuclear | 0.0122 | 0 | 0.0005 | 0.0009 | 0.0028 | 0.0001 | 0.005 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0 | 0 | 0.0003 | 0 | 0.0016 | 0 | 0.0002 |
Vacuole | 0.0022 | 0.0008 | 0.0012 | 0.0013 | 0.0012 | 0.0023 | 0.0021 | 0.0007 | 0.001 | 0.0021 | 0.001 | 0.0008 | 0.0002 | 0.001 | 0.0004 | 0.0019 | 0.0001 | 0.0007 |
Vacuole Periphery | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0019 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.3635 | 15.2291 | 18.9793 | 19.1316 | 16.5659 | 14.6253 | 23.6401 | 23.9165 | 23.1254 | 23.7808 |
Translational Efficiency | 0.4303 | 0.4956 | 0.3145 | 0.3822 | 0.34 | 0.5161 | 0.2975 | 0.3076 | 0.3194 | 0.2717 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
534 | 1222 | 122 | 1402 | 2284 | 1861 | 248 | 61 | 2818 | 3083 | 370 | 1463 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 649.64 | 829.31 | 1123.73 | 937.81 | 838.84 | 833.08 | 938.94 | 916.51 | 802.99 | 831.59 | 999.87 | 936.92 |
Standard Deviation | 86.94 | 107.05 | 123.51 | 127.92 | 96.37 | 104.77 | 111.56 | 104.74 | 120.24 | 105.70 | 144.63 | 127.11 |
Intensity Change Log 2 | — | 0.352271 | 0.790583 | 0.529655 | — | -0.009941 | 0.162637 | 0.127755 | — | 0.159419 | 0.470673 | 0.317050 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.016746 | 0.077280 | 0.001776 | 0.012382 | 0.013842 | 0.032471 | 0.024806 | 0.023450 | 0.014392 | 0.050232 | 0.017212 | 0.012843 |
Bud Neck | 0.050882 | 0.071105 | 0.003623 | 0.010033 | 0.052173 | 0.076238 | 0.007686 | 0.006898 | 0.051928 | 0.074203 | 0.006346 | 0.009903 |
Bud Site | 0.020803 | 0.085543 | 0.018963 | 0.095408 | 0.031683 | 0.067800 | 0.056374 | 0.079328 | 0.029621 | 0.074833 | 0.044039 | 0.094738 |
Cell Periphery | 0.014004 | 0.041126 | 0.002195 | 0.001843 | 0.029157 | 0.024910 | 0.007655 | 0.002580 | 0.026285 | 0.031338 | 0.005855 | 0.001873 |
Cytoplasm | 0.538836* | 0.448261* | 0.243588* | 0.437862* | 0.580403* | 0.508317* | 0.422138* | 0.395548* | 0.572527* | 0.484513* | 0.363265* | 0.436098* |
Cytoplasmic Foci | 0.101007 | 0.073613 | 0.140635 | 0.047009 | 0.060840 | 0.070473 | 0.101304 | 0.105463 | 0.068452 | 0.071718 | 0.114273 | 0.049447 |
Eisosomes | 0.036450 | 0.073912 | 0.000748 | 0.001270 | 0.088204 | 0.078105 | 0.005100 | 0.001281 | 0.078397 | 0.076443 | 0.003665 | 0.001270 |
Endoplasmic Reticulum | 0.002127 | 0.003503 | 0.008053 | 0.002445 | 0.004208 | 0.002946 | 0.006321 | 0.003851 | 0.003814 | 0.003167 | 0.006892 | 0.002504 |
Endosome | 0.003552 | 0.010808 | 0.021794 | 0.008204 | 0.004811 | 0.008785 | 0.004790 | 0.004397 | 0.004572 | 0.009587 | 0.010397 | 0.008045 |
Golgi | 0.005681 | 0.025201 | 0.000596 | 0.006572 | 0.020672 | 0.022102 | 0.000566 | 0.000478 | 0.017831 | 0.023331 | 0.000576 | 0.006318 |
Lipid Particles | 0.011508 | 0.010024 | 0.012017 | 0.002022 | 0.011996 | 0.009314 | 0.003985 | 0.002475 | 0.011904 | 0.009595 | 0.006634 | 0.002041 |
Mitochondria | 0.003544 | 0.006382 | 0.000067 | 0.003767 | 0.003847 | 0.006101 | 0.000109 | 0.000034 | 0.003790 | 0.006213 | 0.000095 | 0.003611 |
Mitotic Spindle | 0.000037 | 0.000819 | 0.000223 | 0.032511 | 0.000179 | 0.002373 | 0.000776 | 0.000087 | 0.000152 | 0.001757 | 0.000594 | 0.031159 |
None | 0.001222 | 0.000406 | 0.000298 | 0.000888 | 0.003743 | 0.000990 | 0.000355 | 0.000171 | 0.003265 | 0.000759 | 0.000336 | 0.000858 |
Nuclear Periphery | 0.000151 | 0.000085 | 0.000600 | 0.000456 | 0.000082 | 0.000067 | 0.000284 | 0.000145 | 0.000095 | 0.000074 | 0.000388 | 0.000443 |
Nuclear Periphery Foci | 0.000218 | 0.000395 | 0.015058 | 0.001881 | 0.000557 | 0.000417 | 0.004049 | 0.002850 | 0.000492 | 0.000408 | 0.007679 | 0.001922 |
Nucleolus | 0.000838 | 0.000273 | 0.000250 | 0.000313 | 0.000262 | 0.000365 | 0.000108 | 0.000052 | 0.000371 | 0.000329 | 0.000155 | 0.000302 |
Nucleus | 0.127101 | 0.016254 | 0.086761 | 0.053455 | 0.045893 | 0.029017 | 0.092496 | 0.057989 | 0.061282 | 0.023958 | 0.090605 | 0.053644 |
Peroxisomes | 0.015361 | 0.015756 | 0.001402 | 0.004042 | 0.009249 | 0.013825 | 0.002691 | 0.002520 | 0.010407 | 0.014591 | 0.002266 | 0.003978 |
Vacuole | 0.049671 | 0.038451 | 0.441132* | 0.273608* | 0.037725 | 0.044752 | 0.258314* | 0.310332* | 0.039989 | 0.042254 | 0.318594* | 0.275140* |
Vacuole Periphery | 0.000260 | 0.000802 | 0.000221 | 0.004029 | 0.000475 | 0.000630 | 0.000092 | 0.000072 | 0.000434 | 0.000698 | 0.000135 | 0.003864 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -11.66 | 4.41 | 2.50 | 16.81* | -5.07 | -10.67 | -1.23 | -0.56 | 1.70 | 0.14 | -17.34* | 0.18 | 4.32 | 18.97* | 1.63 |
Bud Neck | -3.97 | 11.50 | 10.46 | 19.59 | -5.25 | -7.76 | 24.57 | 21.21 | 23.76 | 0.89 | -8.76 | 27.92 | 26.40 | 31.56 | -2.01 |
Bud Site | -15.34 | -0.04 | -9.50 | 3.59 | -8.69 | -11.29 | -2.46 | -2.23 | 0.28 | -0.99 | -18.08 | -2.02 | -8.92 | 1.85 | -4.52 |
Cell Periphery | -8.45 | 7.35 | 7.96 | 14.30 | 3.14 | 2.37 | 8.24 | 20.20 | 16.04 | 1.52 | -2.92 | 10.81 | 23.69 | 21.87 | 2.04 |
Cytoplasm | 7.58 | 15.27 | 12.16 | 6.03 | -8.42 | 10.95 | 11.33 | 6.37 | 4.18 | 0.36 | 15.73 | 17.39 | 22.42 | 11.52 | -3.79 |
Cytoplasmic Foci | 4.35 | -2.27 | 10.47 | 9.13 | 8.80 | -3.34 | -4.48 | -2.42 | -1.65 | -0.03 | -1.28 | -5.96 | 10.14 | 11.18 | 10.39 |
Eisosomes | -7.70 | 10.00 | 9.96 | 21.91 | -0.91 | 2.61 | 29.53 | 33.03 | 26.51 | 2.57 | 0.64 | 31.62 | 34.16 | 34.46 | 2.74 |
Endoplasmic Reticulum | -2.94 | -5.47 | -0.38 | 3.20 | 5.50 | 2.00 | -1.60 | 0.40 | -0.91 | 1.66 | 1.38 | -3.25 | 3.82 | 2.33 | 4.95 |
Endosome | -7.58 | -8.60 | -2.24 | 5.13 | 7.53 | -6.77 | 0.38 | 0.72 | 4.32 | 0.50 | -9.71 | -6.47 | -0.94 | 5.66 | 4.73 |
Golgi | -13.26 | 10.94 | 2.03 | 12.81 | -3.13 | -1.10 | 27.30 | 27.32 | 20.30 | 0.72 | -5.24 | 28.15 | 13.15 | 16.00 | -3.19 |
Lipid Particles | 1.08 | -0.04 | 8.49 | 9.36 | 8.22 | 3.37 | 12.01 | 13.62 | 9.91 | 2.19 | 3.34 | 7.77 | 18.50 | 15.10 | 8.49 |
Mitochondria | -2.64 | 6.43 | 1.74 | 3.80 | -3.22 | -2.68 | 8.38 | 8.52 | 8.48 | 2.35 | -3.56 | 9.82 | 2.50 | 5.07 | -3.18 |
Mitotic Spindle | -1.73 | -1.99 | -7.22 | -6.85 | -7.11 | -2.68 | -1.12 | 1.85 | 2.78 | 1.15 | -3.04 | -1.15 | -7.17 | -6.43 | -4.26 |
None | 3.31 | 3.59 | 1.56 | -1.38 | -1.80 | 5.39 | 7.21 | 7.49 | 3.90 | 2.10 | 6.21 | 7.62 | 5.83 | 0.22 | -1.80 |
Nuclear Periphery | 1.57 | -8.84 | -4.86 | -5.21 | 2.26 | 0.92 | -7.94 | -2.16 | -2.28 | 1.06 | 1.12 | -11.81 | -6.10 | -6.20 | 0.24 |
Nuclear Periphery Foci | -3.75 | -10.34 | -8.91 | -8.01 | 9.18 | 2.07 | -8.48 | -4.77 | -5.02 | 1.53 | 1.68 | -11.76 | -7.95 | -8.50 | 9.19 |
Nucleolus | 0.91 | 0.95 | 0.98 | 0.28 | 0.20 | -0.86 | 10.09 | 10.82 | 2.37 | 2.42 | 0.39 | 1.64 | 0.95 | 0.75 | -0.46 |
Nucleus | 16.17 | 3.17 | 9.83 | -16.50 | 2.22 | 10.29 | -6.56 | -1.32 | -2.61 | 2.14 | 19.78 | -4.56 | 1.52 | -13.81 | 5.10 |
Peroxisomes | -0.19 | 6.40 | 5.84 | 14.59 | -1.94 | -5.35 | 9.18 | 12.17 | 13.12 | 0.17 | -5.56 | 12.20 | 11.78 | 17.05 | -1.04 |
Vacuole | 2.42 | -18.24 | -36.70 | -41.53 | 3.73 | -3.36 | -16.28 | -9.30 | -9.09 | -1.24 | -1.28 | -22.99 | -42.90 | -42.66 | -0.88 |
Vacuole Periphery | -5.13 | 1.46 | -3.01 | -2.14 | -3.08 | -1.97 | 6.70 | 6.93 | 10.02 | 1.27 | -3.76 | 6.39 | -2.71 | -2.27 | -3.21 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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