Standard name
Human Ortholog
Description Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0.05 0.14 0.13 0.15 0.19 – 0.1 0.14 0.13 0.09 0.17 0.19 0.23 0.14 0.13 0.17 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.17 0.05 0.16 0.05 0.45 0.17 – 0.47 0.44 0.5 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.73 0.92 0.72 0.71 0.62 0.64 – 0.66 0.65 0.65 0.72 0.79 0.78 0.72 0.76 0.74 0.73 0.85 0.65 0.73 0.64 0.58 0.51
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0.07 0.1 0.05 0.07 – 0 0 0 0 0 0 0.05 0.11 0.11 0.09 0.07 0.13 0.13 0.2 0.22 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0
Bud 0 2 0 0 0 0 0 – 2 2 4 3 0 0 0 2 0 2 2 3 1 0 8 4
Bud Neck 0 0 1 0 1 0 0 – 0 0 3 0 0 0 0 0 0 0 0 1 0 1 3 2
Bud Site 0 0 0 0 0 6 0 – 6 7 12 9 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0
Cytoplasm 14 39 5 15 17 23 45 – 27 33 32 19 33 45 75 71 60 72 3 20 2 2 3 8
Endoplasmic Reticulum 1 6 1 0 0 0 1 – 0 0 0 0 0 1 1 22 18 12 0 0 0 0 2 2
Endosome 3 4 1 1 1 0 5 – 1 1 2 0 3 2 3 10 12 6 8 22 2 3 8 14
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 2 0 0 5 1 0 1 0 0 0 2
Mitochondria 40 62 5 17 6 68 40 – 123 101 126 97 1 1 0 10 15 16 3 9 1 2 6 8
Nucleus 360 267 100 76 90 93 155 – 174 150 164 154 155 187 230 385 347 313 355 261 96 84 182 139
Nuclear Periphery 0 0 0 1 0 0 0 – 1 4 0 1 0 0 2 1 2 3 0 0 1 1 0 4
Nucleolus 2 0 0 1 1 2 2 – 2 1 1 2 0 1 0 0 0 0 3 11 1 4 12 7
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3 2 0 1 0 6 – 1 5 1 2 1 1 0 1 0 5 7 10 1 2 13 6
Vac/Vac Membrane 11 36 4 7 12 8 18 – 11 7 8 8 7 6 15 54 50 37 30 53 17 26 70 67
Unique Cell Count 398 368 109 105 126 151 242 263 231 253 215 197 241 320 508 470 429 421 405 132 132 315 272
Labelled Cell Count 432 420 119 118 129 200 272 348 311 353 297 201 247 326 559 510 468 421 405 132 132 315 272


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.6 5.0 5.2 5.8 3.9 5.0 – 4.2 4.7 4.4 4.4 6.1 6.3 6.2 6.6 7.4 6.5 5.3 5.3 5.9
Std Deviation (1e-4) 0.7 0.9 0.7 1.7 1.5 1.1 2.0 – 1.1 1.8 1.4 1.3 1.6 2.2 1.8 1.4 1.5 1.5 2.0 1.6 2.0
Intensity Change (Log2) – – – 0.05 0.22 -0.37 0.0 – -0.24 -0.1 -0.18 -0.18 0.28 0.33 0.31 0.4 0.56 0.38 0.08 0.1 0.25

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 2.3 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1218 0.0713 -0.0513 -0.2572 -0.4083 0.0144 2.2305 1.663 1.562 1.3974 1.6426 1.5115 4.0016 4.5565 4.5363 3.9909 3.6335 4.296
Actin 0.0264 0.0001 0.0076 0.0004 0.0006 0.0011 0.0126 0.0001 0.0215 0.0126 0.0057 0 0.0088 0 0.0031 0 0 0
Bud 0.0001 0 0.0001 0 0 0.0001 0.0004 0 0.0001 0.0005 0.0014 0 0.0002 0 0.0001 0 0 0
Bud Neck 0.0004 0.0002 0.0001 0 0.0002 0.0012 0.0106 0.0004 0.0022 0.0006 0.0007 0.0015 0.001 0.0003 0.0055 0.0001 0.0002 0.0013
Bud Periphery 0.0001 0 0.0002 0 0 0.0001 0.0014 0 0.0002 0.0005 0.0041 0.0001 0.0005 0.0001 0.0001 0 0 0
Bud Site 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0044 0.0002 0.0023 0.0043 0.001 0.0001 0.0005 0.0003 0.0003 0 0.0001 0.0001
Cell Periphery 0 0 0 0 0 0 0.0004 0 0.0001 0.0001 0.0005 0 0.0001 0 0 0 0 0
Cytoplasm 0.0217 0.0215 0.0054 0.0012 0.0059 0.0019 0.0126 0.0136 0.0042 0.0012 0.0058 0.002 0.0078 0.0082 0.0053 0.0009 0.0046 0.003
Cytoplasmic Foci 0.003 0.0006 0.0014 0 0.0064 0.0002 0.0128 0.0001 0.008 0.0101 0.0005 0.0001 0.003 0.0001 0.0007 0 0 0.0002
Eisosomes 0.0001 0 0.0004 0 0 0 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0056 0.0009 0.001 0.0005 0.0001 0.0004 0.0033 0.0004 0.0011 0.0003 0.0018 0.0001 0.002 0.0004 0.0002 0 0 0.0001
Endosome 0.0036 0.0014 0.0019 0.0002 0.0034 0.0022 0.0174 0.0003 0.0041 0.0031 0.007 0.0001 0.0083 0.0004 0.0004 0 0 0.0005
Golgi 0.0011 0 0.0024 0 0.0026 0.0014 0.0029 0 0.0024 0.0024 0.0033 0 0.0012 0 0.0007 0 0 0
Lipid Particles 0.0017 0.0001 0.0082 0 0.0359 0.0036 0.0059 0 0.004 0.0058 0.0004 0 0.0019 0 0.0013 0 0 0.0001
Mitochondria 0.0005 0.0003 0.0019 0.0007 0.0006 0.002 0.0042 0.0002 0.0015 0.001 0.0311 0.0002 0.0023 0.0003 0.0003 0.0001 0.0001 0.0001
None 0.0045 0.0098 0.0033 0.0002 0.0108 0.0013 0.0062 0.0117 0.0114 0.0008 0.0285 0.0131 0.0213 0.0208 0.0155 0.0002 0.0435 0.0024
Nuclear Periphery 0.0159 0.0219 0.0237 0.0475 0.0037 0.014 0.0532 0.0053 0.0203 0.0043 0.005 0.0014 0.0123 0.0064 0.0028 0.0029 0.0033 0.0026
Nucleolus 0.0039 0.0082 0.0044 0.0017 0.0083 0.0143 0.0185 0.0063 0.0054 0.0076 0.0189 0.0119 0.0103 0.0081 0.0043 0.0043 0.0189 0.0105
Nucleus 0.9006 0.9193 0.9303 0.9457 0.8519 0.9436 0.7952 0.9533 0.8982 0.9332 0.8444 0.9624 0.9038 0.9475 0.9511 0.9895 0.9261 0.9664
Peroxisomes 0.0043 0 0.0021 0 0.0269 0.0002 0.0117 0 0.0062 0.0059 0.0001 0 0.0015 0 0.0033 0 0 0
Punctate Nuclear 0.0047 0.0139 0.0049 0.0006 0.0425 0.0112 0.0202 0.0065 0.0053 0.0052 0.0357 0.0066 0.0116 0.006 0.0047 0.0019 0.003 0.0122
Vacuole 0.0011 0.0012 0.0002 0.0002 0.0001 0.0004 0.0035 0.0013 0.0007 0.0002 0.0028 0.0003 0.001 0.0007 0.0002 0 0.0001 0.0002
Vacuole Periphery 0.0004 0.0003 0.0003 0.001 0.0001 0.0006 0.0023 0.0002 0.0007 0.0001 0.0014 0.0001 0.0006 0.0002 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.5006 20.8502 13.0915 33.2855 12.7941 30.4837 28.7846 34.4772 37.7076 34.6252
Translational Efficiency 2.6759 3.4957 5.4139 2.3636 5.6369 3.0221 2.6149 2.5569 2.5886 2.2948

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1836 780 347 1911 1943 2144 1972 2019 3779 2924 2319 3930

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.70 719.37 850.61 774.23 659.30 795.16 819.06 969.47 656.09 774.94 823.78 874.53
Standard Deviation 77.38 115.63 101.86 121.75 80.14 106.43 104.09 134.73 78.88 114.00 104.37 161.42
Intensity Change Log 2 — 0.140314 0.382078 0.246342 — 0.270310 0.313034 0.556261 — 0.207102 0.347795 0.410384

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000063 0.000461 0.000476 0.000330 0.000053 0.000504 0.000205 0.000664 0.000058 0.000492 0.000245 0.000501
Bud Neck 0.001929 0.005540 0.004399 0.012424 0.001546 0.006427 0.009479 0.022151 0.001732 0.006191 0.008719 0.017421
Bud Site 0.000479 0.002829 0.000709 0.004405 0.000611 0.004418 0.000733 0.006898 0.000547 0.003994 0.000729 0.005685
Cell Periphery 0.000167 0.000238 0.000106 0.000492 0.000109 0.000191 0.000085 0.000105 0.000137 0.000204 0.000088 0.000293
Cytoplasm 0.040852 0.065412 0.002300 0.067162 0.054814 0.029582 0.009086 0.024496 0.048031 0.039140 0.008071 0.045243
Cytoplasmic Foci 0.007949 0.007332 0.000043 0.005946 0.005108 0.004172 0.000026 0.001652 0.006489 0.005015 0.000028 0.003740
Eisosomes 0.000017 0.000026 0.000021 0.000071 0.000013 0.000019 0.000012 0.000017 0.000015 0.000021 0.000013 0.000043
Endoplasmic Reticulum 0.001551 0.006069 0.005696 0.003403 0.001279 0.003461 0.003694 0.006941 0.001411 0.004157 0.003994 0.005221
Endosome 0.000383 0.001667 0.000188 0.002379 0.000614 0.001135 0.000184 0.002085 0.000502 0.001277 0.000185 0.002228
Golgi 0.000134 0.000698 0.000034 0.001239 0.000423 0.000950 0.000022 0.003801 0.000283 0.000883 0.000024 0.002555
Lipid Particles 0.001302 0.001746 0.000122 0.003122 0.001403 0.001222 0.000040 0.000374 0.001354 0.001362 0.000052 0.001710
Mitochondria 0.001445 0.003020 0.000518 0.004216 0.001194 0.006076 0.000697 0.003513 0.001316 0.005261 0.000670 0.003855
Mitotic Spindle 0.001114 0.004005 0.000121 0.005685 0.000853 0.004137 0.002021 0.014540 0.000980 0.004102 0.001737 0.010234
None 0.009902 0.026550 0.001730 0.019211 0.009329 0.007882 0.002404 0.006149 0.009607 0.012862 0.002303 0.012500
Nuclear Periphery 0.001111 0.001561 0.000378 0.001964 0.000981 0.001862 0.001231 0.002870 0.001044 0.001782 0.001103 0.002430
Nuclear Periphery Foci 0.000234 0.001504 0.000053 0.000984 0.000407 0.000434 0.000066 0.000914 0.000323 0.000719 0.000064 0.000948
Nucleolus 0.004051 0.009395 0.001884 0.003173 0.002597 0.004562 0.000857 0.001742 0.003303 0.005851 0.001011 0.002438
Nucleus 0.925423 0.856037 0.979445 0.854886 0.916778 0.916456 0.968177 0.888727 0.920978 0.900339 0.969863 0.872272
Peroxisomes 0.000875 0.001774 0.000086 0.001576 0.000399 0.001350 0.000022 0.000317 0.000630 0.001463 0.000032 0.000929
Vacuole 0.000863 0.003528 0.001636 0.006279 0.001302 0.003997 0.000909 0.009961 0.001089 0.003872 0.001018 0.008171
Vacuole Periphery 0.000154 0.000609 0.000055 0.001056 0.000188 0.001163 0.000049 0.002082 0.000172 0.001015 0.000050 0.001583

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.12 -11.62 -8.41 0.64 2.41 -2.43 -14.02 -9.41 -1.33 -6.95 -3.18 -18.05 -12.22 -0.74 -6.91
Bud Neck -3.83 -5.84 -9.55 -4.97 -5.23 -5.98 -12.70 -17.23 -11.85 -8.60 -6.91 -13.39 -19.27 -11.81 -7.56
Bud Site -4.43 -2.86 -5.80 -1.81 -5.03 -4.52 -1.02 -6.76 -1.44 -6.62 -5.57 -2.00 -8.90 -1.62 -8.32
Cell Periphery -1.92 1.36 -6.33 -3.62 -5.15 -4.16 2.01 1.62 5.63 -0.97 -3.61 3.70 -5.53 -1.74 -8.22
Cytoplasm -4.40 16.83 -4.67 1.41 -19.92 7.19 14.66 10.35 4.11 -6.96 3.30 19.80 3.99 0.35 -17.95
Cytoplasmic Foci 0.30 7.52 2.94 2.12 -7.55 1.01 7.86 6.12 4.77 -2.87 1.71 10.58 5.68 3.68 -8.03
Eisosomes -4.05 -3.10 -4.79 -3.68 -4.03 -3.57 1.12 -9.19 -2.24 -11.90 -4.32 1.64 -5.87 -4.40 -6.20
Endoplasmic Reticulum -7.80 -10.90 -9.24 2.69 4.02 -7.93 -17.51 -22.75 -12.13 -12.77 -10.65 -19.36 -22.65 -6.97 -7.91
Endosome -3.65 1.87 -3.91 -0.42 -4.56 -2.25 2.83 -2.46 -0.46 -4.84 -4.19 3.52 -4.61 -1.10 -6.50
Golgi -2.42 2.85 -2.02 -0.51 -2.37 -1.05 2.04 -1.94 -1.02 -2.62 -1.64 2.64 -2.66 -0.94 -3.45
Lipid Particles -0.88 7.27 -1.67 -0.76 -3.63 0.35 4.76 3.56 2.74 -3.56 -0.09 7.87 0.03 0.10 -4.19
Mitochondria -1.51 3.32 -2.68 -0.53 -3.94 -4.72 1.10 -4.34 2.73 -7.31 -4.95 2.50 -4.56 2.07 -6.44
Mitotic Spindle -1.90 1.59 -3.98 -1.11 -5.09 -3.14 -1.75 -6.84 -4.55 -5.50 -3.57 -1.44 -7.87 -4.29 -6.03
None -4.57 7.36 -3.22 2.85 -9.24 1.11 6.79 3.86 2.82 -3.90 -2.38 9.70 -0.53 1.92 -9.69
Nuclear Periphery -0.79 5.82 -1.88 -0.67 -3.03 -2.90 -2.30 -9.12 -4.78 -7.29 -2.76 -1.66 -6.26 -2.96 -5.12
Nuclear Periphery Foci -3.41 3.60 -2.20 0.98 -2.76 -0.24 2.55 -1.59 -1.33 -4.52 -2.64 3.61 -2.69 -0.54 -4.23
Nucleolus -4.51 3.91 2.14 5.81 -2.04 -3.31 5.30 0.46 3.16 -2.74 -4.45 7.49 1.92 5.72 -4.65
Nucleus 7.40 -12.33 8.99 -1.34 20.02 0.10 -10.70 4.43 4.26 15.90 4.37 -14.30 9.51 3.95 23.62
Peroxisomes -1.67 3.46 -0.52 1.25 -3.21 -3.42 5.87 1.79 4.00 -2.33 -3.03 4.98 -0.08 2.75 -3.78
Vacuole -5.12 -4.52 -8.41 -4.47 -6.21 -4.56 0.23 -11.24 -8.20 -11.34 -6.23 -2.22 -13.97 -8.85 -13.48
Vacuole Periphery -3.93 2.84 -2.29 -0.71 -2.64 -2.59 3.29 -2.20 0.18 -2.55 -3.04 4.45 -3.11 0.17 -3.62
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Did2

Did2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Did2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available