Standard name | |
---|---|
Human Ortholog | |
Description | WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 2 | 4 | 11 | 3 | 1 | – | 1 | 1 | 3 | 1 | 9 | 6 | 6 | 12 | 1 | 6 | 3 | 3 | 2 | 2 | 4 | 5 |
Bud | 2 | 0 | 0 | 5 | 4 | 9 | 13 | – | 10 | 8 | 14 | 15 | 1 | 3 | 1 | 4 | 9 | 2 | 0 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 4 | – | 2 | 3 | 1 | 3 | 0 | 0 | 0 | 1 | 3 | 1 | – | – | – | – | – | – |
Cell Periphery | 3 | 7 | 0 | 7 | 10 | 6 | 4 | – | 3 | 3 | 0 | 4 | 1 | 7 | 5 | 38 | 26 | 23 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 3 | 3 | 9 | 9 | 11 | – | 11 | 12 | 8 | 22 | 4 | 15 | 22 | 15 | 16 | 15 | 0 | 1 | 0 | 0 | 2 | 1 |
Endoplasmic Reticulum | 4 | 10 | 6 | 4 | 8 | 15 | 2 | – | 8 | 5 | 12 | 6 | 9 | 2 | 9 | 4 | 7 | 8 | 1 | 1 | 1 | 0 | 0 | 1 |
Endosome | 1 | 3 | 0 | 1 | 22 | 13 | 42 | – | 7 | 7 | 0 | 7 | 0 | 12 | 5 | 6 | 18 | 7 | 6 | 5 | 2 | 1 | 8 | 11 |
Golgi | 5 | 21 | 1 | 16 | 33 | 5 | 4 | – | 1 | 1 | 1 | 0 | 10 | 19 | 28 | 122 | 59 | 75 | 6 | 17 | 3 | 2 | 8 | 17 |
Mitochondria | 167 | 107 | 23 | 59 | 137 | 196 | 285 | – | 214 | 235 | 265 | 362 | 70 | 66 | 35 | 26 | 15 | 14 | 141 | 102 | 52 | 28 | 128 | 154 |
Nucleus | 0 | 0 | 2 | 1 | 1 | 4 | 7 | – | 4 | 5 | 6 | 5 | 0 | 1 | 2 | 0 | 4 | 5 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 0 | 4 | 5 | – | 1 | 1 | 3 | 1 | 0 | 0 | 1 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 2 | 0 | 0 | 0 | 1 | 0 | – | 2 | 2 | 6 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 0 | 0 | 10 | 12 | 32 | – | 6 | 1 | 2 | 4 | 0 | 13 | 4 | 18 | 29 | 8 | 8 | 2 | 4 | 3 | 2 | 6 |
SpindlePole | 0 | 0 | 1 | 0 | 9 | 7 | 32 | – | 19 | 15 | 16 | 27 | 0 | 3 | 9 | 8 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 19 | 28 | 4 | 4 | 25 | 20 | 32 | – | 19 | 8 | 7 | 18 | 4 | 13 | 15 | 43 | 44 | 48 | 2 | 11 | 5 | 0 | 20 | 28 |
Unique Cell Count | 170 | 135 | 36 | 74 | 211 | 250 | 380 | 247 | 268 | 288 | 395 | 85 | 119 | 117 | 243 | 206 | 192 | 173 | 150 | 74 | 41 | 181 | 239 | |
Labelled Cell Count | 206 | 189 | 42 | 104 | 279 | 305 | 474 | 310 | 307 | 345 | 477 | 108 | 161 | 142 | 306 | 239 | 221 | 173 | 150 | 74 | 41 | 181 | 239 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 7.1 | 6.2 | 6.9 | 6.8 | 5.5 | 5.4 | – | 4.6 | 4.7 | 4.1 | 4.1 | 6.3 | 7.0 | 7.1 | 8.5 | 8.6 | 7.9 | – | 6.4 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.1 | 0.9 | 1.6 | 1.2 | 1.2 | 1.0 | – | 0.9 | 1.0 | 0.8 | 0.9 | 1.1 | 1.1 | 1.3 | 1.2 | 1.7 | 1.3 | – | 1.0 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.15 | 0.13 | -0.17 | -0.21 | – | -0.44 | -0.41 | -0.6 | -0.59 | 0.01 | 0.17 | 0.18 | 0.46 | 0.47 | 0.34 | – | 0.04 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.6 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 2.8 | 5.3 | 3.3 | 4.2 |
Mitochondria | 1.8 | 0.1 | 1.9 | 1.5 | – | – | – | – | – | 2.2 | -0.9 | -3.7 | -7.8 | -8.6 | -8.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 1.5 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 1.0 | 1.4 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.886 | 1.4908 | 1.0497 | 0.9705 | 0.7166 | 1.2672 | 1.796 | 2.0873 | 2.3165 | 2.2005 | 1.3205 | 2.0079 | 0.9685 | 1.1271 | 1.0251 | 1.3157 | 0.673 | 0.6816 |
Actin | 0.0348 | 0.0302 | 0.0142 | 0.0009 | 0.0826 | 0.0144 | 0.0944 | 0.0444 | 0.04 | 0.0062 | 0.0061 | 0.0154 | 0.0433 | 0.0091 | 0.0221 | 0.0086 | 0.0082 | 0.0204 |
Bud | 0.0011 | 0.003 | 0.0023 | 0.0013 | 0.0013 | 0.0004 | 0.0016 | 0.0042 | 0.0036 | 0.0009 | 0.0015 | 0.0003 | 0.0014 | 0.0052 | 0.0012 | 0.0006 | 0.0013 | 0.0007 |
Bud Neck | 0.0045 | 0.0012 | 0.0058 | 0.0002 | 0.002 | 0.0008 | 0.0043 | 0.0021 | 0.0013 | 0.0139 | 0.0011 | 0.0007 | 0.0021 | 0.0008 | 0.001 | 0.0004 | 0.0009 | 0.0045 |
Bud Periphery | 0.0025 | 0.0055 | 0.0049 | 0.0069 | 0.0029 | 0.0009 | 0.0025 | 0.0066 | 0.008 | 0.0025 | 0.0041 | 0.0007 | 0.0025 | 0.0037 | 0.0019 | 0.0018 | 0.0064 | 0.0019 |
Bud Site | 0.0068 | 0.0388 | 0.0106 | 0.0003 | 0.0035 | 0.0005 | 0.0138 | 0.0579 | 0.0147 | 0.0039 | 0.0021 | 0.0017 | 0.0064 | 0.0148 | 0.0109 | 0.0007 | 0.0008 | 0.0013 |
Cell Periphery | 0.0012 | 0.0025 | 0.004 | 0.0002 | 0.0006 | 0.0004 | 0.0014 | 0.0023 | 0.003 | 0.0007 | 0.0004 | 0.0003 | 0.0009 | 0.0012 | 0.0005 | 0.0003 | 0.0004 | 0.0008 |
Cytoplasm | 0.0039 | 0.0081 | 0.0037 | 0.0002 | 0.0008 | 0.001 | 0.0053 | 0.0049 | 0.0066 | 0.0014 | 0.0013 | 0.0004 | 0.0034 | 0.0078 | 0.0055 | 0.0008 | 0.0011 | 0.0022 |
Cytoplasmic Foci | 0.033 | 0.0304 | 0.0346 | 0.0088 | 0.0201 | 0.0157 | 0.055 | 0.0375 | 0.0257 | 0.0388 | 0.0268 | 0.0106 | 0.0339 | 0.0352 | 0.0299 | 0.0086 | 0.0344 | 0.017 |
Eisosomes | 0.0015 | 0.0009 | 0.0018 | 0.0001 | 0.0007 | 0.0008 | 0.0014 | 0.0007 | 0.002 | 0.0003 | 0.0002 | 0.0005 | 0.0015 | 0.0003 | 0.0008 | 0.0003 | 0.0002 | 0.0007 |
Endoplasmic Reticulum | 0.0026 | 0.0009 | 0.0014 | 0.0001 | 0.0004 | 0.0009 | 0.0015 | 0.0014 | 0.0011 | 0.0003 | 0.0014 | 0.0002 | 0.0036 | 0.0006 | 0.0008 | 0.0005 | 0.0004 | 0.0009 |
Endosome | 0.0806 | 0.083 | 0.0595 | 0.0336 | 0.0367 | 0.0444 | 0.0725 | 0.0709 | 0.0385 | 0.0407 | 0.1148 | 0.0264 | 0.0963 | 0.0611 | 0.0545 | 0.0532 | 0.0696 | 0.039 |
Golgi | 0.0731 | 0.0909 | 0.0386 | 0.0432 | 0.0494 | 0.0625 | 0.0561 | 0.0683 | 0.0507 | 0.0136 | 0.0797 | 0.0463 | 0.0838 | 0.0996 | 0.0705 | 0.0389 | 0.039 | 0.0725 |
Lipid Particles | 0.0339 | 0.0081 | 0.0068 | 0.0064 | 0.0039 | 0.0163 | 0.0347 | 0.0119 | 0.0116 | 0.0131 | 0.0306 | 0.0027 | 0.0449 | 0.0045 | 0.0142 | 0.009 | 0.0277 | 0.0132 |
Mitochondria | 0.626 | 0.6466 | 0.7548 | 0.8864 | 0.7454 | 0.8104 | 0.5848 | 0.6126 | 0.7311 | 0.8247 | 0.6533 | 0.8489 | 0.5632 | 0.7073 | 0.7386 | 0.8529 | 0.7668 | 0.7772 |
None | 0.0297 | 0.0009 | 0.0027 | 0.0001 | 0.0002 | 0.0003 | 0.0083 | 0.0052 | 0.0044 | 0.0014 | 0.0004 | 0.0004 | 0.0221 | 0.0006 | 0.0013 | 0.0005 | 0.0004 | 0.001 |
Nuclear Periphery | 0.003 | 0.0003 | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.002 | 0.0006 | 0.0004 | 0.0002 | 0.0011 | 0.0001 | 0.012 | 0.0002 | 0.001 | 0.0012 | 0.001 | 0.0011 |
Nucleolus | 0.0022 | 0.0005 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0039 | 0.0012 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0019 | 0.0004 | 0.0002 | 0.0004 | 0.0015 | 0.0003 |
Nucleus | 0.0014 | 0.0006 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0022 | 0.0009 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0048 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.0004 |
Peroxisomes | 0.0394 | 0.0347 | 0.0317 | 0.0082 | 0.0346 | 0.0207 | 0.0386 | 0.0452 | 0.0473 | 0.0255 | 0.0595 | 0.0412 | 0.0495 | 0.0357 | 0.037 | 0.0067 | 0.0291 | 0.0359 |
Punctate Nuclear | 0.0017 | 0.0002 | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0071 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0 | 0.0069 | 0.0001 | 0.0012 | 0.0008 | 0.0008 | 0.0028 |
Vacuole | 0.0111 | 0.009 | 0.0135 | 0.0012 | 0.0066 | 0.0047 | 0.0053 | 0.0166 | 0.0056 | 0.0061 | 0.0104 | 0.0011 | 0.008 | 0.0074 | 0.0041 | 0.0064 | 0.0062 | 0.0027 |
Vacuole Periphery | 0.0061 | 0.0039 | 0.0058 | 0.0014 | 0.0077 | 0.0037 | 0.0032 | 0.0045 | 0.0031 | 0.0049 | 0.0037 | 0.0018 | 0.0079 | 0.0041 | 0.0024 | 0.0069 | 0.0034 | 0.0036 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.1358 | 12.2531 | 10.9866 | 10.9804 | 13.3287 | 11.9869 | 14.583 | 15.6784 | 10.4573 | 15.9409 |
Translational Efficiency | 0.5756 | 0.4657 | 0.5427 | 0.4029 | 0.4275 | 0.462 | 0.3901 | 0.2986 | 0.3969 | 0.4212 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1899 | 1339 | 373 | 918 | 1923 | 1742 | 416 | 1110 | 3822 | 3081 | 789 | 2028 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 706.57 | 796.80 | 1052.24 | 841.58 | 765.09 | 802.16 | 1008.28 | 865.18 | 736.01 | 799.83 | 1029.06 | 854.50 |
Standard Deviation | 81.75 | 125.06 | 121.86 | 127.60 | 103.49 | 109.87 | 106.09 | 109.94 | 97.80 | 116.75 | 115.92 | 118.84 |
Intensity Change Log 2 | — | 0.173385 | 0.574559 | 0.252268 | — | 0.068261 | 0.398195 | 0.177371 | — | 0.119689 | 0.485564 | 0.213816 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002719 | 0.007585 | 0.066377 | 0.052344 | 0.004750 | 0.004749 | 0.016567 | 0.026564 | 0.003741 | 0.005982 | 0.040115 | 0.038234 |
Bud Neck | 0.003425 | 0.002627 | 0.009775 | 0.021448 | 0.002238 | 0.001756 | 0.011640 | 0.015701 | 0.002828 | 0.002134 | 0.010758 | 0.018303 |
Bud Site | 0.007880 | 0.014390 | 0.111528* | 0.205843* | 0.010565 | 0.007289 | 0.118360* | 0.153587* | 0.009231 | 0.010375 | 0.115130* | 0.177241* |
Cell Periphery | 0.000552 | 0.000645 | 0.000815 | 0.000826 | 0.000868 | 0.000459 | 0.000263 | 0.000504 | 0.000711 | 0.000540 | 0.000524 | 0.000650 |
Cytoplasm | 0.002961 | 0.003259 | 0.014359 | 0.075775 | 0.002434 | 0.002288 | 0.038125 | 0.065200 | 0.002696 | 0.002710 | 0.026889 | 0.069987 |
Cytoplasmic Foci | 0.029814 | 0.035408 | 0.128920 | 0.122872 | 0.027536 | 0.032011 | 0.156642 | 0.137201 | 0.028668 | 0.033487 | 0.143536 | 0.130715 |
Eisosomes | 0.000134 | 0.000132 | 0.000281 | 0.000311 | 0.000113 | 0.000116 | 0.000155 | 0.000210 | 0.000123 | 0.000123 | 0.000214 | 0.000256 |
Endoplasmic Reticulum | 0.000617 | 0.000714 | 0.012364 | 0.007859 | 0.000423 | 0.000391 | 0.008470 | 0.002383 | 0.000519 | 0.000531 | 0.010311 | 0.004862 |
Endosome | 0.049546 | 0.070727 | 0.087424 | 0.057345 | 0.048603 | 0.063072 | 0.076045 | 0.069374 | 0.049071 | 0.066399 | 0.081424 | 0.063929 |
Golgi | 0.106653 | 0.112116* | 0.166961* | 0.065664 | 0.092311 | 0.127567* | 0.077770 | 0.086780 | 0.099437 | 0.120852* | 0.119935* | 0.077222 |
Lipid Particles | 0.003671 | 0.003539 | 0.002961 | 0.004295 | 0.001033 | 0.003546 | 0.004280 | 0.002252 | 0.002344 | 0.003543 | 0.003656 | 0.003177 |
Mitochondria | 0.747113* | 0.680201* | 0.105456* | 0.130982* | 0.761102* | 0.707489* | 0.094230 | 0.184230* | 0.754151* | 0.695630* | 0.099537 | 0.160126* |
Mitotic Spindle | 0.003496 | 0.007702 | 0.116393* | 0.051856 | 0.004828 | 0.005422 | 0.158918* | 0.069657 | 0.004166 | 0.006413 | 0.138814* | 0.061599 |
None | 0.000459 | 0.000264 | 0.000185 | 0.000574 | 0.000180 | 0.000166 | 0.000362 | 0.000333 | 0.000319 | 0.000209 | 0.000278 | 0.000442 |
Nuclear Periphery | 0.000081 | 0.000081 | 0.000298 | 0.000511 | 0.000028 | 0.000053 | 0.000826 | 0.000388 | 0.000055 | 0.000065 | 0.000576 | 0.000444 |
Nuclear Periphery Foci | 0.000154 | 0.000224 | 0.001983 | 0.001155 | 0.000095 | 0.000126 | 0.002362 | 0.000554 | 0.000124 | 0.000169 | 0.002183 | 0.000826 |
Nucleolus | 0.000503 | 0.000415 | 0.000392 | 0.001258 | 0.000208 | 0.000877 | 0.002601 | 0.000541 | 0.000355 | 0.000677 | 0.001557 | 0.000865 |
Nucleus | 0.000331 | 0.001067 | 0.004765 | 0.014263 | 0.000201 | 0.000370 | 0.017194 | 0.007147 | 0.000266 | 0.000673 | 0.011318 | 0.010368 |
Peroxisomes | 0.019607 | 0.033912 | 0.086265 | 0.082671 | 0.028282 | 0.019596 | 0.095728 | 0.075481 | 0.023972 | 0.025818 | 0.091254 | 0.078735 |
Vacuole | 0.006736 | 0.011343 | 0.065505 | 0.090360 | 0.005062 | 0.009106 | 0.104372 | 0.086596 | 0.005894 | 0.010079 | 0.085997 | 0.088300 |
Vacuole Periphery | 0.013550 | 0.013647 | 0.016995 | 0.011788 | 0.009139 | 0.013548 | 0.015092 | 0.015317 | 0.011331 | 0.013592 | 0.015991 | 0.013719 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.01 | -8.61 | -11.55 | -10.06 | 1.79 | 0.00 | -5.03 | -7.44 | -7.38 | -2.65 | -2.94 | -9.54 | -13.62 | -12.42 | 0.61 |
Bud Neck | 0.94 | -4.02 | -8.61 | -9.31 | -4.65 | 0.84 | -8.65 | -11.74 | -11.85 | -2.38 | 1.35 | -8.43 | -13.54 | -14.44 | -5.15 |
Bud Site | -3.64 | -13.22 | -23.95 | -22.83 | -7.91 | 2.42 | -12.96 | -22.09 | -22.62 | -3.03 | -1.08 | -18.46 | -32.40 | -31.96 | -7.56 |
Cell Periphery | -0.85 | -2.20 | -2.43 | -1.34 | 0.19 | 2.55 | 3.90 | 2.43 | -0.42 | -5.21 | 1.78 | 1.94 | 0.96 | -1.43 | -1.67 |
Cytoplasm | -0.49 | -6.69 | -16.19 | -16.03 | -13.01 | 0.25 | -11.84 | -18.51 | -18.45 | -6.02 | -0.14 | -13.20 | -24.38 | -24.26 | -13.23 |
Cytoplasmic Foci | -2.73 | -14.96 | -18.97 | -17.32 | 0.95 | -2.33 | -17.77 | -22.97 | -21.76 | 2.14 | -3.45 | -23.13 | -29.74 | -27.79 | 2.19 |
Eisosomes | 0.12 | -6.14 | -7.82 | -9.35 | -1.08 | -0.36 | -3.66 | -7.93 | -6.90 | -3.40 | 0.01 | -6.93 | -10.50 | -11.54 | -2.56 |
Endoplasmic Reticulum | -0.89 | -5.74 | -8.07 | -7.96 | 1.98 | 0.39 | -5.98 | -8.97 | -9.56 | 4.45 | -0.19 | -8.16 | -10.17 | -10.17 | 4.23 |
Endosome | -5.34 | -6.63 | -1.36 | 3.52 | 5.32 | -4.29 | -4.81 | -4.94 | -1.02 | 1.32 | -6.76 | -8.02 | -4.64 | 1.63 | 4.52 |
Golgi | -0.89 | -5.34 | 8.05 | 8.39 | 9.53 | -6.12 | 2.62 | 1.98 | 7.41 | -1.02 | -5.09 | -2.63 | 6.86 | 11.06 | 6.65 |
Lipid Particles | 0.08 | 0.54 | -0.94 | -0.97 | -1.74 | -3.27 | -4.23 | -3.69 | 1.21 | 2.31 | -1.80 | -2.51 | -2.17 | 0.11 | 0.73 |
Mitochondria | 5.91 | 53.20* | 62.95* | 47.60* | -1.43 | 5.19 | 60.11* | 56.84* | 48.56* | -7.09 | 7.69 | 80.19* | 83.52* | 67.85* | -6.24 |
Mitotic Spindle | -4.07 | -10.58 | -10.94 | -9.90 | 5.65 | -0.67 | -12.72 | -13.88 | -13.64 | 6.62 | -3.32 | -16.49 | -17.65 | -16.83 | 8.62 |
None | 1.35 | 1.93 | -0.61 | -1.41 | -1.71 | 0.76 | -2.37 | -2.20 | -2.40 | 0.12 | 1.53 | 0.45 | -1.14 | -2.14 | -1.51 |
Nuclear Periphery | 0.02 | -4.57 | -5.44 | -5.52 | -2.59 | -3.88 | -4.25 | -4.18 | -3.95 | 1.80 | -0.85 | -5.15 | -6.55 | -6.45 | 0.70 |
Nuclear Periphery Foci | -1.43 | -2.89 | -5.81 | -5.36 | 1.14 | -1.02 | -4.44 | -5.83 | -5.58 | 3.28 | -1.62 | -5.12 | -7.98 | -7.51 | 3.05 |
Nucleolus | 0.22 | 0.17 | -1.77 | -2.22 | -2.33 | -1.28 | -2.92 | -2.25 | 0.42 | 2.42 | -0.96 | -2.70 | -2.49 | -0.80 | 1.48 |
Nucleus | -1.40 | -2.69 | -8.28 | -7.67 | -4.02 | -0.91 | -5.14 | -6.39 | -6.24 | 2.46 | -1.62 | -5.77 | -10.42 | -9.89 | -0.13 |
Peroxisomes | -4.23 | -7.78 | -11.60 | -8.01 | 0.60 | 2.91 | -7.67 | -9.67 | -11.71 | 2.15 | -0.87 | -10.95 | -15.13 | -14.00 | 2.00 |
Vacuole | -3.92 | -8.44 | -17.07 | -16.10 | -4.39 | -4.71 | -13.10 | -18.87 | -17.98 | 1.17 | -5.92 | -15.35 | -25.42 | -24.15 | -1.89 |
Vacuole Periphery | -0.06 | -1.30 | 1.82 | 1.73 | 2.23 | -4.29 | -3.12 | -4.11 | -0.69 | 0.02 | -2.64 | -2.84 | -1.70 | 0.63 | 1.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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