ORF
Human Ortholog
Description Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0.06 0 0.05
Bud Neck 0 0 – 0 – 0 0 0 0 0.05 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 – 0 0 0 0 0 – 0.06 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.24 – 0.68 – 0.49 0.4 0.3 0.26 0.28 – 0.24 0.31 0.51 0.52 – 0.42 0.39 0.18 0.16 0.28 0.37 0.38 0.44
Endoplasmic Reticulum 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0.08 0 0 0 0 0 0 0
Golgi 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.29 – 0.07 – 0.32 0.34 0.29 0.62 0.58 – 0.6 0 0 0 – 0 0.07 0 0 0.09 0 0.05 0
Nucleus 0.34 0.52 – 0.17 – 0.24 0.36 0.42 0.44 0.45 – 0.46 0.57 0.38 0.37 – 0.25 0.2 0.42 0.51 0.19 0.2 0.22 0.16
Nuclear Periphery 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0.07 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0.05 0.05 0.07 0.06 0.09
Vac/Vac Membrane 0.37 0.1 – 0.05 – 0.11 0.05 0 0.07 0 – 0 0.12 0.16 0.14 – 0.34 0.3 0.24 0.19 0.29 0.17 0.17 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 1 0 0 1 2
Bud 0 0 – 0 – 0 0 0 2 1 – 0 0 0 0 – 0 0 4 5 0 4 7 11
Bud Neck 0 1 – 0 – 0 1 0 0 5 – 0 0 0 0 – 0 0 1 0 0 1 4 3
Bud Site 0 0 – 0 – 0 0 0 2 3 – 6 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 55 59 – 27 – 47 34 20 24 29 – 26 30 78 109 – 64 39 42 54 14 29 94 110
Endoplasmic Reticulum 0 1 – 0 – 0 0 0 0 0 – 0 1 1 0 – 9 5 0 0 0 0 2 3
Endosome 1 0 – 1 – 0 0 1 0 0 – 0 0 3 2 – 12 4 4 3 1 0 4 3
Golgi 0 0 – 0 – 0 0 0 0 0 – 0 1 0 0 – 0 1 0 0 0 1 0 0
Mitochondria 17 71 – 3 – 30 29 19 56 60 – 65 1 0 3 – 5 7 9 12 4 1 12 7
Nucleus 64 130 – 7 – 23 31 28 40 47 – 50 55 58 77 – 38 20 102 167 10 15 55 41
Nuclear Periphery 0 1 – 0 – 0 0 0 1 1 – 2 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 – 1 0 1 1 0 – 0 0 0 0 – 0 1 0 0 0 0 0 3
Peroxisomes 0 0 – 0 – 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 1 2 – 3 – 1 0 2 0 0 – 3 0 0 2 – 2 0 10 15 2 5 15 21
Vac/Vac Membrane 70 24 – 2 – 10 4 2 6 0 – 2 12 25 30 – 51 30 58 63 15 12 43 29
Unique Cell Count 188 248 40 95 86 66 91 104 108 96 153 209 151 99 244 329 54 78 252 251
Labelled Cell Count 208 289 43 112 99 73 132 146 154 100 165 223 181 107 244 329 54 78 252 251


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.5 – 5.0 – 4.2 4.2 4.4 4.2 3.8 – 4.1 5.2 5.2 4.7 4.3 5.8 6.3 5.2 5.8 6.2
Std Deviation (1e-4) 0.7 0.4 – 1.8 – 0.9 1.7 0.9 2.2 1.5 – 1.7 0.9 1.1 0.9 1.0 1.9 2.2 1.4 1.7 2.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6397 -0.1831 -0.5715 -0.8203 -0.8167 -0.4015 3.7536 4.0163 4.2235 4.1475 3.2818 3.9185 1.1869 1.298 1.1869 1.5501 1.0086 1.3106
Actin 0.0002 0.0001 0.0002 0.0003 0.0003 0.0001 0.0062 0.0034 0.0082 0.0029 0.0069 0.0007 0.009 0.0001 0.0005 0.0041 0.0002 0.0028
Bud 0 0.0003 0.0011 0 0 0 0.0007 0.0002 0.0002 0.0002 0.0005 0.0001 0.0004 0.0001 0.0002 0.0021 0.0001 0.0001
Bud Neck 0.0006 0.0005 0.0003 0.0003 0.0006 0.0025 0.0033 0.0007 0.0025 0.0031 0.002 0.0072 0.0054 0.0006 0.0015 0.0013 0.0015 0.0023
Bud Periphery 0 0.0001 0.0002 0 0 0 0.0014 0.0003 0.0002 0.0004 0.0007 0.0003 0.0009 0.0001 0.0002 0.001 0.0001 0.0001
Bud Site 0.0003 0.0009 0.0014 0 0.0001 0.0001 0.0027 0.0008 0.0011 0.0005 0.0008 0.0002 0.0079 0.0008 0.001 0.0014 0.0001 0.0001
Cell Periphery 0 0 0 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0002 0 0
Cytoplasm 0.0233 0.0331 0.0432 0.0208 0.0268 0.0163 0.0757 0.0993 0.0773 0.0736 0.0709 0.076 0.0766 0.0745 0.058 0.0373 0.0644 0.0448
Cytoplasmic Foci 0.0048 0.0069 0.0016 0.0008 0.0035 0.0016 0.0115 0.0033 0.0033 0.003 0.0218 0.002 0.0114 0.0006 0.0045 0.0287 0.0008 0.0016
Eisosomes 0 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0003 0.0003 0.0001 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.001 0.0014 0.0014 0.0013 0.0018 0.0004 0.0052 0.0032 0.0028 0.0041 0.0021 0.0021 0.0033 0.0022 0.0017 0.0028 0.0032 0.0009
Endosome 0.0027 0.0061 0.0017 0.0008 0.0019 0.0021 0.0164 0.0033 0.0027 0.0168 0.0123 0.0021 0.0142 0.001 0.0055 0.0091 0.0016 0.002
Golgi 0.0003 0.0001 0 0 0.0001 0.0001 0.0023 0.0006 0.0005 0.0014 0.0118 0.0002 0.0032 0 0.0007 0.0029 0 0.0005
Lipid Particles 0.0032 0.0015 0.0003 0.0001 0.0003 0.0006 0.0072 0.0011 0.0007 0.0016 0.0071 0.0003 0.0108 0.0001 0.0027 0.0085 0.0001 0.0014
Mitochondria 0.0004 0.0003 0.0003 0.0002 0.0004 0.0003 0.0059 0.0026 0.0047 0.0008 0.0177 0.0056 0.006 0.0004 0.0008 0.001 0.0005 0.0004
None 0.0651 0.094 0.079 0.0849 0.1242 0.0451 0.1918 0.1071 0.3018 0.2045 0.0887 0.1139 0.1418 0.1002 0.2142 0.1315 0.1706 0.0973
Nuclear Periphery 0.0146 0.0191 0.0196 0.0236 0.0257 0.0066 0.0246 0.0135 0.0157 0.0186 0.0149 0.0114 0.0144 0.013 0.0103 0.0058 0.0172 0.0064
Nucleolus 0.0099 0.0051 0.0067 0.0054 0.0137 0.0138 0.0089 0.0073 0.0055 0.0083 0.0067 0.0119 0.014 0.0044 0.0051 0.0079 0.0077 0.0101
Nucleus 0.8251 0.801 0.7988 0.8199 0.7314 0.8713 0.5779 0.7078 0.5232 0.6266 0.6649 0.718 0.619 0.7858 0.6632 0.7021 0.716 0.799
Peroxisomes 0.0005 0.0088 0.0001 0 0.0001 0.0002 0.0066 0.0016 0.0017 0.0003 0.013 0.0002 0.0194 0 0.0039 0.0047 0 0.0006
Punctate Nuclear 0.0468 0.0193 0.0431 0.041 0.0682 0.0383 0.0445 0.0409 0.0459 0.0295 0.0552 0.0464 0.037 0.0136 0.0218 0.0448 0.013 0.0286
Vacuole 0.0007 0.0011 0.0007 0.0003 0.0006 0.0005 0.0056 0.0027 0.0014 0.0025 0.0014 0.0012 0.0039 0.0022 0.0036 0.0022 0.002 0.0008
Vacuole Periphery 0.0002 0.0004 0.0003 0.0002 0.0003 0.0001 0.0016 0.0004 0.0004 0.0009 0.0006 0.0003 0.0009 0.0002 0.0006 0.0005 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.9307 7.8596 11.6643 2.633 5.6996 21.968 6.9535 5.8105 5.8328 4.2848
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1680 889 2441 1596 1948 1638 138 1722 3628 2527 2579 3318

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 625.54 751.36 838.24 824.81 670.31 815.70 932.40 805.18 649.58 793.07 843.28 814.62
Standard Deviation 74.37 98.91 95.48 104.68 92.88 93.47 97.53 106.07 87.70 100.24 97.91 105.86
Intensity Change Log 2 — 0.264402 0.422261 0.398960 — 0.283210 0.476121 0.264483 — 0.274162 0.450372 0.330964

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000386 0.000183 0.000646 0.000043 0.000101 0.000763 0.000398 0.000050 0.000201 0.000214 0.000517
Bud Neck 0.002443 0.005632 0.010834 0.009301 0.001198 0.005944 0.001687 0.008536 0.001775 0.005834 0.010345 0.008904
Bud Site 0.001103 0.002404 0.003252 0.013328 0.000353 0.001776 0.000722 0.014361 0.000700 0.001997 0.003117 0.013864
Cell Periphery 0.000259 0.000157 0.000105 0.000152 0.000140 0.000152 0.000114 0.000227 0.000195 0.000154 0.000106 0.000191
Cytoplasm 0.184852* 0.136207* 0.052518 0.125544* 0.118389* 0.134807* 0.027260 0.145877* 0.149165* 0.135299* 0.051167 0.136096*
Cytoplasmic Foci 0.041559 0.024239 0.000400 0.006443 0.009301 0.016609 0.001783 0.002216 0.024239 0.019293 0.000474 0.004250
Eisosomes 0.000050 0.000042 0.000014 0.000034 0.000019 0.000029 0.000075 0.000025 0.000033 0.000034 0.000017 0.000029
Endoplasmic Reticulum 0.001108 0.004901 0.003168 0.004815 0.000779 0.001280 0.010306 0.004031 0.000931 0.002554 0.003550 0.004408
Endosome 0.001079 0.002022 0.000560 0.004624 0.000478 0.001902 0.000924 0.001140 0.000756 0.001944 0.000580 0.002816
Golgi 0.000360 0.001131 0.000037 0.001740 0.000081 0.000755 0.000070 0.000444 0.000210 0.000887 0.000039 0.001068
Lipid Particles 0.005437 0.004391 0.000250 0.001168 0.001211 0.002867 0.001791 0.000615 0.003168 0.003403 0.000333 0.000881
Mitochondria 0.002403 0.004081 0.001089 0.007469 0.000489 0.005171 0.000573 0.001704 0.001375 0.004788 0.001062 0.004477
Mitotic Spindle 0.000111 0.002785 0.002605 0.032805 0.000053 0.003069 0.000144 0.005268 0.000080 0.002969 0.002474 0.018514
None 0.025076 0.014171 0.003804 0.008503 0.023958 0.008038 0.004402 0.010698 0.024476 0.010196 0.003836 0.009642
Nuclear Periphery 0.001071 0.001854 0.001820 0.003504 0.000782 0.001864 0.001387 0.001935 0.000916 0.001860 0.001797 0.002690
Nuclear Periphery Foci 0.000740 0.001280 0.000175 0.001807 0.000283 0.000521 0.000794 0.000559 0.000494 0.000788 0.000208 0.001159
Nucleolus 0.005147 0.005286 0.000895 0.001723 0.004850 0.004657 0.003145 0.001046 0.004988 0.004878 0.001016 0.001372
Nucleus 0.721406* 0.777324* 0.912052* 0.745911* 0.835546* 0.804020* 0.925793* 0.781869* 0.782692* 0.794628* 0.912788* 0.764573*
Peroxisomes 0.001916 0.003262 0.000081 0.000723 0.000290 0.001630 0.000381 0.000126 0.001043 0.002204 0.000097 0.000413
Vacuole 0.003546 0.007384 0.005915 0.027921 0.001646 0.004376 0.017745 0.017258 0.002526 0.005434 0.006548 0.022387
Vacuole Periphery 0.000277 0.001060 0.000240 0.001839 0.000113 0.000432 0.000142 0.001668 0.000189 0.000653 0.000235 0.001750

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.10 -11.51 -6.18 -2.34 -4.27 -2.28 -8.40 -12.60 -8.72 4.79 -6.59 -15.13 -10.12 -6.32 -5.19
Bud Neck -4.30 -7.27 -10.86 -4.87 0.15 -5.71 -3.67 -10.03 -2.86 -7.39 -6.74 -8.07 -14.76 -5.05 0.41
Bud Site -2.49 -2.77 -6.07 -5.08 -4.51 -3.84 -5.24 -6.78 -5.79 -6.15 -3.93 -4.12 -9.08 -7.72 -6.45
Cell Periphery 1.75 2.96 2.56 2.82 -1.60 -0.55 -0.51 -2.53 -1.61 -1.81 1.39 3.39 1.39 -0.11 -3.63
Cytoplasm 5.52 23.43 11.83 4.02 -11.92 -2.51 10.64 -1.72 0.82 -11.75 2.66 25.42 6.92 3.64 -18.91
Cytoplasmic Foci 5.04 17.26 15.31 8.43 -6.10 -4.37 6.59 7.85 10.24 0.32 2.93 19.31 16.73 12.91 -7.71
Eisosomes 1.43 13.66 1.03 -0.64 -13.42 -4.22 -8.09 -8.07 -1.74 6.71 -0.39 7.16 -3.62 -2.59 -11.18
Endoplasmic Reticulum -6.45 -17.64 -21.18 -3.54 -9.74 -4.89 -9.57 -17.79 -15.16 6.06 -7.35 -21.25 -27.55 -12.90 -6.54
Endosome -3.41 3.04 -3.44 -0.96 -4.61 -4.09 -3.88 -4.66 2.04 1.46 -4.81 0.54 -4.86 0.41 -5.04
Golgi -1.34 4.19 -0.90 0.71 -2.06 -2.46 -0.48 -1.35 1.45 -1.30 -2.51 4.59 -1.46 1.29 -2.56
Lipid Particles 1.09 10.42 8.47 4.49 -7.71 -4.15 -3.30 3.13 5.60 4.90 -0.51 11.35 8.89 6.91 -7.73
Mitochondria -1.63 3.03 -2.12 -0.27 -3.96 -4.69 -3.26 -7.06 3.53 -5.71 -4.53 1.24 -3.47 2.22 -4.41
Mitotic Spindle -3.20 -5.30 -8.31 -6.92 -6.91 -3.01 -1.18 -5.74 -2.24 -5.38 -4.08 -5.38 -9.88 -6.99 -7.07
None 4.11 10.70 8.80 4.52 -3.75 7.75 7.09 7.93 -0.16 -1.68 8.95 15.07 11.82 2.95 -6.12
Nuclear Periphery -3.19 -13.29 -13.48 -8.64 -8.82 -6.03 -5.42 -12.49 -3.93 -3.90 -6.54 -16.70 -17.80 -9.48 -8.37
Nuclear Periphery Foci -1.79 3.00 -1.26 0.25 -2.81 -0.99 -4.05 -2.62 -0.79 0.99 -1.49 2.03 -2.52 -0.68 -3.49
Nucleolus -0.67 7.71 6.68 5.60 -2.35 0.10 1.30 12.54 5.21 2.63 -0.17 11.70 12.24 7.16 -0.33
Nucleus -4.44 -24.52 -3.43 1.54 20.17 3.79 -3.39 5.84 1.85 6.31 -1.60 -23.31 1.57 3.03 24.60
Peroxisomes -2.10 6.38 5.01 4.67 -3.49 -4.80 -1.59 4.15 5.47 5.30 -3.64 6.97 5.48 6.81 -3.59
Vacuole -4.19 -7.05 -17.12 -14.81 -15.24 -3.71 -4.58 -14.58 -13.00 1.57 -5.37 -9.20 -21.94 -19.66 -16.28
Vacuole Periphery -1.74 0.81 -2.96 0.50 -3.13 -4.27 -3.50 -1.72 -1.12 -1.62 -2.81 -0.05 -2.52 -0.84 -2.41
ORF
Human Ortholog
Description Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
Localization
Cell Percentages nucleus (74%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YKR041W

YKR041W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr041w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available