Standard name
Human Ortholog
Description Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.93 0.93 0.82 0.81 0.87 0.82 0.74 0.81 1.0 0.99 1.0 0.98 0.98 0.96 0.97 0.94 0.95 0.94 0.96 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.13 0.13 0 0.09 0.12 0.13 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 4 5 3 4 8 0 0 0 0 0 2 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 2 1 1 1 0 0 0 0 0 0 0 0 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 1 0 0 11 5 10 13 16 8 12 0 0 0 2 1 3 0 0 0 0 0 0
Cytoplasm 233 165 350 282 282 347 359 393 467 406 191 292 207 270 243 342 228 235 228 166 332 148 188 208
Endoplasmic Reticulum 1 1 4 3 10 15 17 18 21 21 9 5 0 1 0 16 9 6 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0
Golgi 1 0 0 0 0 0 3 2 1 5 0 0 1 1 0 0 0 2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 5 7 15 5 29 14 0 0 0 1 1 2 1 0 1 0 0 0
Nucleus 0 0 2 2 2 9 15 15 15 21 6 8 0 3 0 6 6 0 0 3 3 5 3 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 5 3 8 15 4 57 64 24 44 31 46 1 3 0 0 4 15 3 3 1 0 0 1
Unique Cell Count 235 169 356 289 304 373 439 488 538 495 257 362 208 274 243 349 232 246 236 178 348 159 197 222
Labelled Cell Count 236 171 360 295 309 388 463 514 563 522 279 387 209 278 243 368 250 265 236 178 348 159 197 222


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.1 16.5 20.7 20.6 15.2 17.7 16.2 15.9 14.5 14.6 13.1 13.4 21.0 19.9 18.6 22.7 29.2 29.7 21.6 24.0 23.1
Std Deviation (1e-4) 4.2 3.8 3.6 3.6 3.4 3.1 3.0 2.9 2.9 2.7 3.0 3.3 4.3 3.7 3.4 5.5 6.6 7.5 5.0 4.5 4.6
Intensity Change (Log2) -0.01 -0.45 -0.23 -0.36 -0.38 -0.51 -0.5 -0.66 -0.63 0.02 -0.05 -0.16 0.13 0.49 0.52 0.06 0.21 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.8 0 0 2.5 3.0 0 3.0 0 0 0 0 0 0
Cytoplasm -0.7 -3.5 -3.5 -7.5 -7.9 -6.0 -7.4 -9.1 -7.7 1.2 0.2 2.0 -0.3 0 -2.0
Endoplasmic Reticulum 0 1.9 2.5 2.4 2.3 2.5 2.7 2.0 0 0 0 0 2.8 2.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.2 0 6.5 3.7 0 0 0 0 0 0
Nucleus 0 0 2.0 2.8 2.6 2.4 3.3 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.2 0 6.4 6.5 3.1 5.1 6.0 6.3 0 0 0 0 0 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.41 17.7188 15.3762 15.8767 13.9103 16.4797 24.5315 27.6594 26.3219 27.1501 25.92 25.7723 17.7232 20.1576 19.3908 19.7536 17.3152 18.8994
Actin 0.0129 0.0006 0.0211 0.002 0.039 0.0081 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0083 0.0003 0.007 0.0001 0.0007 0.0015
Bud 0.0003 0.0001 0.0012 0.0001 0.001 0.0003 0.0001 0.0001 0.0005 0 0 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0001
Bud Neck 0.0006 0.0002 0.0009 0.0005 0.0033 0.0011 0.0002 0.0002 0.0002 0.0003 0.0003 0.0006 0.0003 0.0002 0.0003 0.0003 0.0009 0.0009
Bud Periphery 0.0004 0 0.0015 0 0.0017 0.0002 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Bud Site 0.0006 0.0006 0.0027 0.0003 0.0044 0.0006 0.0001 0.0001 0 0 0 0 0.0003 0.0009 0.0001 0 0.0001 0
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8654 0.9683 0.8973 0.9182 0.7527 0.9023 0.9469 0.9805 0.9626 0.9705 0.9621 0.9497 0.8992 0.9787 0.9435 0.9782 0.9512 0.937
Cytoplasmic Foci 0.0067 0.001 0.0034 0.0112 0.0226 0.0085 0.0007 0.0004 0.0004 0.0002 0.0006 0.0011 0.0101 0.0025 0.0015 0.0002 0.001 0.0012
Eisosomes 0.0001 0 0.0001 0.0001 0.0012 0.0001 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0037 0.0011 0.0026 0.0048 0.0035 0.004 0.0014 0.0008 0.0006 0.0007 0.0011 0.0013 0.0023 0.0007 0.0007 0.0004 0.0022 0.0036
Endosome 0.0114 0.0005 0.0038 0.0056 0.0247 0.0101 0.0007 0.0002 0.0001 0.0001 0.0004 0.001 0.0052 0.0019 0.0005 0.0001 0.0006 0.0021
Golgi 0.0031 0 0.0026 0.001 0.0123 0.0044 0.0001 0 0 0 0 0.0001 0.0016 0.0004 0.0003 0 0 0.0002
Lipid Particles 0.0065 0 0.0007 0.0107 0.0286 0.0026 0.0001 0 0 0 0 0.0001 0.0036 0 0.0005 0 0 0.0002
Mitochondria 0.0198 0.0001 0.0129 0.0005 0.046 0.0028 0.0004 0.0001 0.0001 0 0.0001 0.0004 0.0014 0.0002 0.0003 0 0.0001 0.0002
None 0.0015 0.0006 0.0024 0.0041 0.0019 0.0005 0.0008 0.0004 0.0005 0.0003 0.0004 0.0004 0.0017 0.0005 0.001 0.0002 0.0013 0.0006
Nuclear Periphery 0.0142 0.0063 0.0071 0.0049 0.0057 0.0058 0.0046 0.0015 0.0023 0.0029 0.0033 0.0036 0.0054 0.0014 0.0026 0.0017 0.0038 0.0075
Nucleolus 0.0004 0 0.0008 0.0002 0.0019 0.0001 0 0 0 0 0 0.0001 0.0001 0 0 0 0 0.0001
Nucleus 0.0437 0.0193 0.0319 0.0319 0.0225 0.0444 0.0426 0.0151 0.0319 0.0242 0.0307 0.0398 0.0513 0.0111 0.0388 0.0184 0.0361 0.0414
Peroxisomes 0.0026 0 0.0009 0.0004 0.0117 0.001 0 0 0 0 0 0.0001 0.006 0 0.0019 0 0 0.0001
Punctate Nuclear 0.0037 0.0004 0.0037 0.002 0.0112 0.0008 0.0004 0.0002 0.0003 0.0002 0.0004 0.0008 0.0016 0.0002 0.0005 0.0001 0.0013 0.0026
Vacuole 0.0016 0.0006 0.0015 0.001 0.0024 0.0016 0.0004 0.0003 0.0002 0.0004 0.0003 0.0004 0.0007 0.0004 0.0002 0.0002 0.0005 0.0005
Vacuole Periphery 0.0008 0.0002 0.0006 0.0004 0.0012 0.0005 0.0002 0 0 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 110.2052 136.5938 162.3529 123.8532 155.1583 113.5229 143.3042 159.6201 141.6662 120.9914
Translational Efficiency 2.5238 2.6652 1.6557 2.3097 1.8598 2.1704 2.136 1.7184 1.5649 1.8366

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
162 1464 2073 1011 173 2249 2285 781 335 3713 4358 1792

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1263.81 1983.15 2054.22 2401.11 1598.40 2008.81 2116.22 2312.81 1436.60 1998.69 2086.73 2362.63
Standard Deviation 239.14 678.58 336.55 467.64 273.86 326.83 405.83 458.96 307.15 496.41 375.76 465.94
Intensity Change Log 2 0.650014 0.700811 0.925922 0.329713 0.404861 0.533018 0.479968 0.543070 0.719798

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000881 0.000157 0.000119 0.001213 0.000652 0.000192 0.000148 0.000142 0.000763 0.000179 0.000134 0.000746
Bud Neck 0.002939 0.000544 0.000882 0.008212 0.001109 0.000498 0.001579 0.000830 0.001994 0.000516 0.001247 0.004995
Bud Site 0.021798 0.001383 0.000690 0.023574 0.003565 0.002486 0.002607 0.001312 0.012382 0.002051 0.001695 0.013871
Cell Periphery 0.000485 0.000102 0.000093 0.000460 0.000342 0.000047 0.000086 0.000190 0.000411 0.000069 0.000089 0.000342
Cytoplasm 0.807695* 0.750778* 0.579355* 0.545155* 0.741039* 0.803624* 0.619201* 0.637960* 0.773273* 0.782787* 0.600247* 0.585602*
Cytoplasmic Foci 0.042734 0.004853 0.001910 0.018482 0.017510 0.006041 0.001959 0.005483 0.029708 0.005573 0.001935 0.012816
Eisosomes 0.000010 0.000001 0.000001 0.000016 0.000006 0.000001 0.000003 0.000001 0.000008 0.000001 0.000002 0.000010
Endoplasmic Reticulum 0.002164 0.000216 0.000083 0.000610 0.000968 0.000145 0.000528 0.000069 0.001546 0.000173 0.000316 0.000374
Endosome 0.001137 0.000684 0.000063 0.018306 0.000816 0.000837 0.000437 0.000427 0.000971 0.000777 0.000259 0.010514
Golgi 0.001561 0.000460 0.000117 0.015958 0.000591 0.000688 0.000245 0.000518 0.001060 0.000598 0.000184 0.009229
Lipid Particles 0.001094 0.000655 0.000209 0.008041 0.000727 0.000235 0.000285 0.000406 0.000905 0.000400 0.000249 0.004713
Mitochondria 0.000174 0.000121 0.000319 0.013407 0.000112 0.000168 0.000320 0.000125 0.000142 0.000150 0.000319 0.007618
Mitotic Spindle 0.002117 0.000811 0.003981 0.060754 0.000621 0.002979 0.004884 0.002755 0.001344 0.002124 0.004454 0.035476
None 0.003207 0.016389 0.011854 0.009168 0.005259 0.013029 0.012821 0.012173 0.004267 0.014354 0.012361 0.010477
Nuclear Periphery 0.000099 0.000077 0.000269 0.001127 0.000175 0.000059 0.000272 0.000295 0.000138 0.000066 0.000270 0.000764
Nuclear Periphery Foci 0.005196 0.001425 0.000445 0.006439 0.010343 0.001067 0.000684 0.001844 0.007854 0.001208 0.000570 0.004436
Nucleolus 0.000185 0.000252 0.000135 0.001266 0.000091 0.000112 0.000194 0.000285 0.000136 0.000167 0.000166 0.000838
Nucleus 0.092488 0.216591* 0.394256* 0.218903* 0.199494* 0.163079* 0.333511* 0.325142* 0.147748* 0.184178* 0.362406* 0.265205*
Peroxisomes 0.000277 0.000032 0.000009 0.000752 0.000048 0.000151 0.000044 0.000019 0.000159 0.000104 0.000028 0.000433
Vacuole 0.013306 0.004051 0.005004 0.037700 0.016412 0.004124 0.019684 0.009232 0.014910 0.004095 0.012701 0.025293
Vacuole Periphery 0.000452 0.000418 0.000207 0.010459 0.000119 0.000436 0.000509 0.000792 0.000280 0.000429 0.000365 0.006246

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.15 5.11 2.07 -5.25 -5.23 4.66 5.69 4.63 -1.04 -2.33 6.75 7.53 4.05 -4.15 -5.70
Bud Neck 3.00 1.68 -3.08 -10.78 -8.27 4.10 -3.91 -2.34 -8.84 2.02 4.17 -0.04 -4.13 -12.90 -6.32
Bud Site 4.01 4.05 1.97 -7.78 -8.05 2.26 1.79 0.73 -2.41 -1.71 4.25 4.23 1.77 -7.77 -7.88
Cell Periphery 3.64 3.19 -0.92 -12.20 -10.80 5.77 4.12 0.41 -11.74 -7.79 6.38 4.97 -0.77 -17.09 -13.34
Cytoplasm 4.57 18.54 15.27 19.39 -3.23 -4.69 11.33 6.95 24.99 -7.76 -1.04 20.27 15.27 32.17 -6.99
Cytoplasmic Foci 6.26 6.69 3.96 -10.32 -12.89 5.74 7.15 2.38 -6.37 -9.64 7.91 8.85 4.39 -11.96 -16.13
Eisosomes 4.44 4.08 0.34 -8.83 -8.23 4.37 2.11 2.75 -7.25 2.21 6.25 4.42 1.64 -10.69 -6.19
Endoplasmic Reticulum 4.45 4.72 4.65 0.52 -0.96 4.73 3.55 5.22 3.35 4.50 6.00 5.74 6.31 1.30 3.49
Endosome 1.20 5.95 -2.55 -2.91 -4.16 0.72 3.97 4.22 2.08 1.04 1.43 6.99 -1.70 -2.34 -3.94
Golgi 2.21 2.89 -0.98 -2.42 -2.80 0.01 6.20 3.01 1.34 -1.70 1.87 3.87 -0.88 -1.99 -2.92
Lipid Particles 1.09 1.84 -6.03 -6.43 -7.23 4.31 1.27 -2.70 -6.34 -4.15 3.09 2.23 -6.62 -8.26 -7.99
Mitochondria 1.71 -5.68 -2.23 -2.30 -1.95 -1.14 -7.75 -0.55 0.97 8.40 -0.13 -9.16 -2.25 -2.24 -1.76
Mitotic Spindle 1.48 -3.94 -4.91 -5.39 -3.11 -2.88 -5.68 -3.13 -1.07 1.07 -1.31 -6.65 -5.73 -5.16 -2.56
None -12.29 -14.38 -6.10 9.29 10.24 -7.15 -6.58 -4.47 3.88 2.77 -13.39 -12.57 -6.51 9.95 8.75
Nuclear Periphery 0.23 -18.14 -9.08 -9.30 -2.04 5.40 -11.83 -3.03 -6.34 4.24 4.96 -19.82 -8.87 -11.33 0.66
Nuclear Periphery Foci 2.33 2.93 1.05 -4.63 -7.32 6.63 6.76 5.65 -3.94 -4.61 6.42 6.87 4.71 -6.41 -8.56
Nucleolus -0.35 0.07 -1.03 -0.49 -1.31 -1.05 -6.84 -1.24 -0.58 1.71 -0.49 -2.04 -1.52 -0.95 -0.42
Nucleus -12.97 -31.40 -10.89 2.15 28.52 2.25 -11.62 -6.14 -17.41 9.35 -4.59 -25.79 -9.96 -11.19 26.71
Peroxisomes 3.13 3.33 0.82 -4.10 -4.49 -1.11 2.30 4.03 1.56 1.17 1.14 3.57 0.83 -0.58 -4.17
Vacuole 3.24 -1.83 -20.52 -22.83 -20.72 4.71 -6.60 -5.72 -13.76 0.12 5.65 -6.78 -19.00 -25.65 -16.25
Vacuole Periphery 0.34 1.99 -2.05 -2.08 -2.60 -1.12 -0.80 -0.66 0.47 -0.21 -0.58 1.14 -2.24 -1.70 -2.53
Standard name
Human Ortholog
Description Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Shb17

Shb17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Shb17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available