ORF
Human Ortholog
Description Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.05 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.12 0.09 – 0.2 0 0 0 – 0.06 0.06 0.08 0.05 0.33 0.29 0.28 0.2 0.24 0.17 0.13 0.09 0.34 0.24 0.3 0.24
Cytoplasm 0.97 0.99 – 0.98 0.98 0.97 0.96 – 0.87 0.89 0.84 0.8 0.98 0.99 0.99 0.94 0.97 0.94 0.68 0.77 0.55 0.62 0.56 0.62
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.08 0.07 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.1 0 – 0.23 0.18 0.29 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 – 1 3 0 0 – 1 0 0 0 0 0 1 0 0 2 6 1 0 4 10 11
Bud 3 3 – 1 2 5 0 – 1 4 6 9 1 3 3 1 0 0 0 0 0 1 6 2
Bud Neck 3 0 – 3 4 0 7 – 2 2 0 0 4 1 1 2 0 1 13 7 0 11 22 22
Bud Site 0 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 30 6 – 40 11 5 11 – 17 21 22 15 101 166 170 44 41 26 32 8 6 60 155 129
Cytoplasm 247 66 – 198 285 182 385 – 256 294 228 249 301 562 592 204 168 148 177 72 10 156 292 337
Endoplasmic Reticulum 1 0 – 0 1 1 0 – 0 0 0 0 1 0 0 13 10 13 19 1 0 6 14 21
Endosome 0 0 – 1 0 0 1 – 0 0 0 0 0 0 1 1 0 0 1 0 0 1 1 3
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 1 0 0 1 4 0
Mitochondria 3 1 – 1 0 19 16 – 67 61 80 99 0 0 2 2 0 2 2 1 0 2 5 4
Nucleus 1 0 – 0 1 4 6 – 5 1 5 5 0 0 0 4 1 3 0 0 0 0 0 0
Nuclear Periphery 2 0 – 0 0 0 0 – 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 1 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 2 – 2 2 1 6 0 1 1 1 0 1 0 0 0 0 1 0
Vac/Vac Membrane 0 0 – 0 0 0 3 – 0 0 0 2 0 3 1 1 2 1 1 0 0 1 2 1
Unique Cell Count 254 67 202 292 188 401 294 331 273 312 306 568 601 216 173 157 260 94 19 253 525 542
Labelled Cell Count 291 76 245 307 216 432 351 387 343 386 408 737 773 274 222 198 260 94 19 253 525 542


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.7 – 5.5 5.6 3.8 5.0 – 4.1 4.3 4.3 4.4 6.1 6.2 6.4 6.6 7.3 6.5 5.6 5.9 5.9
Std Deviation (1e-4) 0.6 0.9 – 1.0 1.3 1.0 1.8 – 1.1 1.2 1.4 2.2 0.9 1.1 1.4 1.2 1.2 1.0 1.3 1.5 1.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5974 0.5487 0.2051 -0.2269 -0.4608 0.1539 0.6729 1.1092 0.7885 1.0944 1.1514 1.1329 2.7862 2.6431 2.8602 2.833 3.1195 2.7933
Actin 0.062 0.0006 0.0072 0.0012 0.0197 0.0245 0.0419 0.0026 0.0041 0.004 0.0367 0.0073 0.0307 0.0037 0.0006 0.0029 0.0082 0.0062
Bud 0.0008 0.0002 0.0018 0.0004 0.0002 0.0009 0.0007 0.001 0.0011 0.0003 0.0005 0.0003 0.0009 0.0002 0.0004 0.0002 0.0026 0.0002
Bud Neck 0.0041 0.0013 0.0019 0.0015 0.001 0.0153 0.0035 0.0012 0.0104 0.0054 0.001 0.0171 0.001 0.0006 0.0014 0.0007 0.0011 0.0207
Bud Periphery 0.0011 0.0005 0.0093 0.0009 0.0004 0.0029 0.0013 0.0012 0.0017 0.0007 0.0012 0.0007 0.0052 0.0005 0.0016 0.0005 0.0031 0.0004
Bud Site 0.01 0.0258 0.0099 0.0018 0.0008 0.0008 0.0056 0.0297 0.0269 0.0048 0.0003 0.0003 0.0046 0.0097 0.0047 0.0006 0.0073 0.0014
Cell Periphery 0.0142 0.006 0.0083 0.0037 0.0014 0.0046 0.0105 0.0105 0.0093 0.0072 0.0017 0.0039 0.0126 0.0149 0.0121 0.0127 0.0054 0.0031
Cytoplasm 0.365 0.3203 0.453 0.42 0.299 0.5221 0.541 0.6209 0.7251 0.5744 0.6169 0.7498 0.5578 0.6055 0.694 0.6109 0.6144 0.7207
Cytoplasmic Foci 0.0387 0.0023 0.0079 0.0279 0.0207 0.0165 0.0202 0.0083 0.0035 0.0165 0.0091 0.0045 0.0352 0.0047 0.0015 0.003 0.0098 0.002
Eisosomes 0.0009 0.0005 0.0009 0.0008 0.0002 0.0004 0.0005 0.0006 0.0006 0.0006 0.0012 0.0003 0.0003 0.0003 0.0003 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.009 0.0032 0.0078 0.0096 0.0039 0.0028 0.0054 0.0026 0.006 0.0038 0.0099 0.0025 0.0053 0.0026 0.0044 0.0033 0.0039 0.0022
Endosome 0.0422 0.0002 0.008 0.0041 0.0259 0.0015 0.0102 0.0021 0.0007 0.0097 0.0277 0.0026 0.0218 0.0025 0.0006 0.0046 0.007 0.0007
Golgi 0.0172 0.0001 0.0034 0.0006 0.0088 0.0013 0.003 0.0003 0.0005 0.0069 0.0111 0.0008 0.0048 0.0108 0.0001 0.0065 0.0049 0.0003
Lipid Particles 0.0254 0.0003 0.0103 0.0035 0.0208 0.0039 0.0102 0.001 0.0008 0.0194 0.0023 0.0007 0.0117 0.0008 0.0005 0.0092 0.0058 0.0005
Mitochondria 0.0094 0.0001 0.0036 0.0004 0.0019 0.0008 0.0007 0.0002 0.0002 0.0073 0.0068 0.0002 0.001 0.0019 0.0001 0.0269 0.0028 0.0001
None 0.326 0.636 0.4535 0.5089 0.5489 0.3929 0.3236 0.3134 0.2033 0.3043 0.2625 0.2047 0.2674 0.3368 0.2645 0.3133 0.2785 0.2394
Nuclear Periphery 0.0122 0.0002 0.0031 0.0008 0.008 0.0007 0.0013 0.0004 0.0005 0.0005 0.0061 0.0006 0.0074 0.0002 0.0051 0.0002 0.0009 0.0005
Nucleolus 0.0023 0.0003 0.0005 0.001 0.0005 0.0001 0.0006 0.0003 0.001 0.001 0.0001 0.0002 0.0008 0.0002 0.0001 0.0001 0.0002 0
Nucleus 0.0144 0.0008 0.0026 0.0015 0.0013 0.0005 0.0013 0.0007 0.0014 0.0008 0.0008 0.0006 0.0037 0.0007 0.0063 0.0004 0.0008 0.0003
Peroxisomes 0.0125 0.0001 0.0032 0.0006 0.0097 0.0057 0.0068 0.0002 0.0002 0.0271 0.0007 0.0006 0.0083 0.0014 0 0.0024 0.0406 0.0002
Punctate Nuclear 0.0266 0.0004 0.0015 0.0085 0.0255 0.0009 0.0088 0.0011 0.0011 0.0034 0.0009 0.002 0.0163 0.0002 0.0003 0.0001 0.001 0.0004
Vacuole 0.0043 0.0008 0.002 0.0022 0.001 0.001 0.0027 0.0016 0.0016 0.0016 0.0014 0.0006 0.0027 0.0017 0.0013 0.0012 0.0014 0.0004
Vacuole Periphery 0.0016 0 0.0004 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0003 0.001 0.0001 0.0004 0 0.0001 0.0004 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9924 14.2953 15.9116 12.571 15.5501 9.9891 13.0989 15.2429 14.322 14.5593
Translational Efficiency 0.9382 1.0389 0.9045 0.8937 1.0486 1.4069 1.1868 0.8519 0.9988 1.0597

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1293 1091 154 1314 1192 1919 226 1328 2485 3010 380 2642

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 746.29 802.68 1083.16 904.79 738.83 841.06 997.39 933.75 742.71 827.15 1032.15 919.35
Standard Deviation 89.02 105.37 130.10 113.90 88.72 93.80 109.99 116.38 88.95 99.87 125.80 116.06
Intensity Change Log 2 — 0.105089 0.537438 0.277847 — 0.186966 0.432915 0.337794 — 0.146403 0.486385 0.307981

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000712 0.002998 0.002837 0.005232 0.000516 0.001353 0.002004 0.002322 0.000618 0.001949 0.002342 0.003770
Bud Neck 0.039654 0.024970 0.014136 0.008568 0.013806 0.039140 0.005058 0.011629 0.027255 0.034004 0.008737 0.010107
Bud Site 0.012917 0.042508 0.030551 0.043286 0.011577 0.027654 0.026526 0.064926 0.012274 0.033038 0.028158 0.054163
Cell Periphery 0.001761 0.001456 0.003459 0.002137 0.001109 0.001169 0.001555 0.000896 0.001448 0.001273 0.002327 0.001513
Cytoplasm 0.542212* 0.551092* 0.244684* 0.433942* 0.609642* 0.578819* 0.355526* 0.446214* 0.574557* 0.568769* 0.310606* 0.440111*
Cytoplasmic Foci 0.168052 0.167377 0.045767 0.040266 0.164748 0.110830 0.070445 0.043694 0.166467 0.131326 0.060444 0.041989
Eisosomes 0.002820 0.001334 0.000468 0.000797 0.001015 0.001720 0.000360 0.000239 0.001954 0.001580 0.000404 0.000516
Endoplasmic Reticulum 0.002640 0.003900 0.030451 0.005329 0.001608 0.001012 0.011884 0.003395 0.002145 0.002059 0.019408 0.004357
Endosome 0.010224 0.011886 0.008438 0.005488 0.008394 0.005628 0.006339 0.009816 0.009346 0.007896 0.007190 0.007664
Golgi 0.005474 0.004514 0.000283 0.003731 0.002605 0.003023 0.000220 0.004077 0.004098 0.003563 0.000246 0.003905
Lipid Particles 0.012371 0.004905 0.008494 0.002251 0.004791 0.003565 0.005133 0.001972 0.008735 0.004051 0.006495 0.002111
Mitochondria 0.004160 0.001518 0.000280 0.000835 0.001478 0.001757 0.000093 0.003700 0.002873 0.001671 0.000169 0.002275
Mitotic Spindle 0.002552 0.000759 0.000289 0.005324 0.000179 0.002941 0.000213 0.026369 0.001414 0.002150 0.000244 0.015902
None 0.001554 0.001960 0.000444 0.000793 0.001489 0.001591 0.001041 0.002888 0.001522 0.001725 0.000799 0.001846
Nuclear Periphery 0.000215 0.000129 0.001272 0.000547 0.000124 0.000097 0.000565 0.000782 0.000171 0.000108 0.000852 0.000665
Nuclear Periphery Foci 0.000564 0.001624 0.014519 0.002998 0.001250 0.000413 0.012535 0.002399 0.000893 0.000852 0.013339 0.002697
Nucleolus 0.000353 0.000497 0.000142 0.000071 0.000306 0.000351 0.000131 0.000305 0.000330 0.000404 0.000136 0.000188
Nucleus 0.113200 0.084406 0.240367* 0.136439* 0.101470 0.147271* 0.149302* 0.112707 0.107574 0.124485 0.186208* 0.124510
Peroxisomes 0.005584 0.004851 0.000459 0.001412 0.001852 0.004026 0.000510 0.002914 0.003794 0.004325 0.000489 0.002167
Vacuole 0.072344 0.086381 0.352499* 0.299129* 0.071627 0.067148 0.350446* 0.254356* 0.072000 0.074119 0.351278* 0.276624*
Vacuole Periphery 0.000637 0.000935 0.000159 0.001424 0.000415 0.000493 0.000113 0.004401 0.000530 0.000653 0.000131 0.002920

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.72 -8.13 -4.07 -0.70 -0.94 -5.58 -5.29 -3.96 -0.52 1.61 -8.48 -8.55 -5.16 -1.42 -0.25
Bud Neck 4.66 7.10 12.25 9.00 3.17 -11.12 7.20 3.86 14.12 -3.88 -3.27 10.11 12.53 16.92 1.05
Bud Site -9.64 -4.87 -6.51 2.88 -0.42 -8.32 -3.92 -8.76 -3.78 -3.57 -12.26 -5.89 -10.86 -1.44 -3.08
Cell Periphery 2.35 -3.11 1.33 -0.28 3.55 -0.72 -1.96 5.72 6.98 5.03 2.38 -3.14 3.72 2.12 5.02
Cytoplasm -0.77 19.77 18.57 17.96 -8.74 3.80 17.15 23.51 21.58 -3.30 0.99 24.07 29.44 28.99 -7.34
Cytoplasmic Foci 0.11 21.95 29.32 26.82 1.62 10.69 16.81 30.34 23.53 6.75 8.81 26.00 42.03 35.08 6.52
Eisosomes 7.09 13.92 10.93 4.46 -1.19 -6.15 10.13 14.00 15.30 4.74 3.07 16.29 14.18 12.28 -0.02
Endoplasmic Reticulum -2.04 -8.71 -4.71 -2.16 7.87 1.26 -8.66 -3.64 -11.92 7.65 0.20 -11.53 -5.85 -9.00 10.18
Endosome -1.48 2.90 7.74 7.24 4.19 2.60 2.53 2.46 0.35 0.50 1.92 4.21 6.47 4.72 3.00
Golgi 1.36 8.17 3.87 3.35 -2.62 -0.91 7.76 1.44 2.09 -3.18 1.22 10.60 4.10 3.49 -4.03
Lipid Particles 7.61 4.35 11.64 5.59 7.64 2.42 -0.20 6.84 4.67 6.73 8.36 4.41 13.25 7.03 9.98
Mitochondria 2.10 3.19 2.98 2.20 -1.05 -0.36 1.97 -0.35 -0.05 -2.79 1.57 3.78 2.19 1.14 -3.04
Mitotic Spindle 1.78 2.17 -1.16 -2.89 -3.23 -2.76 -0.69 -6.66 -5.20 -6.63 -0.89 2.12 -6.24 -5.55 -7.36
None -0.74 3.77 2.84 2.46 -2.06 -0.32 1.12 -0.82 -0.61 -1.32 -0.67 2.68 0.31 0.75 -1.47
Nuclear Periphery 3.19 -10.17 -4.42 -5.31 4.89 2.24 -9.04 -11.64 -12.05 -3.88 4.37 -12.57 -10.38 -11.50 1.41
Nuclear Periphery Foci -7.73 -8.53 -11.79 -4.43 7.03 9.43 -9.15 -6.32 -13.91 8.31 0.38 -12.59 -13.18 -13.11 10.85
Nucleolus -1.06 7.63 12.94 3.02 3.86 -0.70 4.06 1.53 2.17 -2.19 -1.20 7.72 5.93 4.13 -0.53
Nucleus 6.17 -7.40 -5.40 -10.55 5.69 -9.60 -4.68 -5.00 3.60 2.00 -4.85 -8.28 -7.27 -2.58 5.34
Peroxisomes 1.11 10.39 8.56 8.82 -1.82 -4.96 5.96 0.92 4.70 -2.32 -1.37 11.79 7.17 8.74 -2.91
Vacuole -3.99 -16.20 -38.94 -35.95 -0.15 1.61 -19.12 -34.45 -36.04 1.39 -0.99 -25.10 -51.77 -51.79 0.89
Vacuole Periphery -1.21 3.71 -0.41 0.52 -2.31 -0.45 3.14 -2.32 -2.17 -2.83 -0.84 4.91 -2.26 -1.88 -3.50
ORF
Human Ortholog
Description Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

YKR045C

YKR045C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr045c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available