Standard name | |
---|---|
Human Ortholog | |
Description | Histone chaperone; involved in histone exchange by removing and replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; involved in the transport of H2A and H2B histones to the nucleus; required for the regulation of microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 0 | 4 | 5 | 2 | 3 | 8 | 11 | 11 | 6 | 11 | 10 | 0 | 2 | 0 | 0 | 1 | 2 | 4 | 1 | 4 | 1 | 1 | 2 |
Bud | 8 | 4 | 1 | 0 | 1 | 1 | 8 | 2 | 1 | 4 | 2 | 4 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 95 | 59 | 100 | 35 | 29 | 40 | 55 | 74 | 53 | 42 | 42 | 55 | 202 | 167 | 147 | 128 | 118 | 99 | 7 | 5 | 23 | 1 | 1 | 0 |
Cytoplasm | 450 | 216 | 230 | 146 | 156 | 207 | 239 | 275 | 156 | 192 | 164 | 229 | 633 | 639 | 657 | 363 | 352 | 238 | 443 | 212 | 201 | 375 | 391 | 545 |
Endoplasmic Reticulum | 24 | 4 | 18 | 11 | 16 | 14 | 39 | 47 | 41 | 26 | 20 | 40 | 28 | 23 | 21 | 12 | 10 | 8 | 24 | 3 | 37 | 9 | 8 | 20 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 1 | 1 | 0 | 3 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 1 | 0 | 12 | 27 | 18 | 19 | 26 | 19 | 14 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 1 | 0 | 2 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 5 | 2 | 1 | 1 | 0 | 3 | 3 | 4 |
Unique Cell Count | 487 | 225 | 268 | 165 | 177 | 232 | 311 | 350 | 229 | 243 | 222 | 293 | 665 | 660 | 678 | 412 | 403 | 276 | 493 | 232 | 280 | 403 | 417 | 594 |
Labelled Cell Count | 588 | 284 | 355 | 199 | 204 | 279 | 380 | 430 | 282 | 298 | 261 | 354 | 869 | 836 | 830 | 509 | 495 | 353 | 493 | 232 | 280 | 403 | 417 | 594 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.2 | 22.3 | 18.6 | 18.3 | 16.9 | 16.0 | 15.4 | 15.4 | 14.6 | 14.9 | 14.5 | 14.0 | 23.2 | 23.1 | 22.8 | 30.9 | 27.0 | 29.4 | 24.8 | 25.5 | 27.0 |
Std Deviation (1e-4) | 3.4 | 4.6 | 4.2 | 3.2 | 2.7 | 2.4 | 2.9 | 3.2 | 3.1 | 4.1 | 3.0 | 2.8 | 4.3 | 4.1 | 4.1 | 9.3 | 7.3 | 8.7 | 5.2 | 5.5 | 5.3 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.14 | -0.22 | -0.27 | -0.28 | -0.35 | -0.32 | -0.36 | -0.41 | 0.32 | 0.31 | 0.29 | 0.73 | 0.54 | 0.66 | 0.41 | 0.45 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0.9 | 1.3 | 2.2 | 0 | 2.2 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -3.5 | -4.8 | -5.0 | -5.3 | -4.4 | -3.4 | -5.0 | -4.5 | -4.9 | -2.0 | -3.7 | -4.9 | -1.7 | -2.2 | -0.3 |
Cytoplasm | 0.8 | 0.7 | 1.1 | -2.7 | -2.3 | -4.7 | -2.0 | -3.3 | -2.4 | 4.9 | 6.2 | 6.4 | 0.9 | 0.6 | 0.1 |
Endoplasmic Reticulum | 0 | 0.9 | -0.3 | 2.3 | 2.7 | 3.8 | 1.6 | 0.9 | 2.7 | -1.6 | -2.2 | -2.5 | -2.4 | -2.7 | -2.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.4 | 4.6 | 3.4 | 4.5 | 5.2 | 4.6 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.8525 | 26.4712 | 25.2358 | 25.0542 | 24.1246 | 24.151 | 19.2729 | 28.8749 | 26.3331 | 23.8876 | 20.8622 | 25.39 | 24.0042 | 24.8214 | 25.5811 | 23.5076 | 23.3208 | 24.9091 |
Actin | 0.0062 | 0.003 | 0.0012 | 0.0002 | 0.0052 | 0.0034 | 0.0363 | 0.0017 | 0.0035 | 0.0324 | 0.0484 | 0.0053 | 0.0021 | 0.0041 | 0.0006 | 0.0174 | 0.0049 | 0.0101 |
Bud | 0.0005 | 0.0003 | 0.0009 | 0.0005 | 0.0011 | 0.0007 | 0.0009 | 0.001 | 0.0017 | 0.0049 | 0.0007 | 0.0004 | 0.0003 | 0.0006 | 0.0004 | 0.0043 | 0.0004 | 0.0007 |
Bud Neck | 0.0025 | 0.0013 | 0.0019 | 0.0009 | 0.0169 | 0.0742 | 0.005 | 0.0013 | 0.0019 | 0.0019 | 0.0007 | 0.0984 | 0.004 | 0.0017 | 0.0015 | 0.0118 | 0.0077 | 0.1154 |
Bud Periphery | 0.0011 | 0.0004 | 0.001 | 0.0002 | 0.0015 | 0.0025 | 0.0016 | 0.0008 | 0.0014 | 0.0055 | 0.0016 | 0.0012 | 0.0011 | 0.0004 | 0.0002 | 0.0047 | 0.0005 | 0.0022 |
Bud Site | 0.0117 | 0.0231 | 0.0022 | 0.0002 | 0.0004 | 0.0004 | 0.0042 | 0.0081 | 0.0031 | 0.0049 | 0.001 | 0.0012 | 0.0012 | 0.0208 | 0.0009 | 0.0031 | 0.001 | 0.0005 |
Cell Periphery | 0.033 | 0.0135 | 0.0073 | 0.0005 | 0.0074 | 0.0314 | 0.0225 | 0.0235 | 0.0029 | 0.0007 | 0.0007 | 0.011 | 0.0306 | 0.0134 | 0.0037 | 0.0119 | 0.0024 | 0.0208 |
Cytoplasm | 0.8706 | 0.9201 | 0.9286 | 0.9245 | 0.9097 | 0.8437 | 0.7037 | 0.9416 | 0.9151 | 0.8305 | 0.7391 | 0.8305 | 0.8775 | 0.9247 | 0.9654 | 0.7081 | 0.9428 | 0.8145 |
Cytoplasmic Foci | 0.0049 | 0.0108 | 0.0096 | 0.0139 | 0.0071 | 0.0027 | 0.0291 | 0.001 | 0.0089 | 0.0107 | 0.0044 | 0.0086 | 0.0025 | 0.0038 | 0.0031 | 0.0416 | 0.0056 | 0.0019 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0 |
Endoplasmic Reticulum | 0.0365 | 0.0136 | 0.014 | 0.0068 | 0.0109 | 0.0258 | 0.0464 | 0.0113 | 0.0072 | 0.0026 | 0.0916 | 0.019 | 0.0485 | 0.0186 | 0.005 | 0.0303 | 0.0114 | 0.0202 |
Endosome | 0.0054 | 0.0028 | 0.0044 | 0.025 | 0.0118 | 0.0016 | 0.0292 | 0.0003 | 0.0112 | 0.0225 | 0.0217 | 0.0032 | 0.0028 | 0.0016 | 0.0031 | 0.0174 | 0.0056 | 0.002 |
Golgi | 0.0016 | 0.0009 | 0.0009 | 0.0021 | 0.0025 | 0.0011 | 0.0051 | 0.0003 | 0.0056 | 0.0047 | 0.0109 | 0.0011 | 0.0008 | 0.0007 | 0.0002 | 0.0033 | 0.0011 | 0.0011 |
Lipid Particles | 0.0078 | 0.0052 | 0.0035 | 0.0005 | 0.0016 | 0.0054 | 0.0163 | 0.0019 | 0.0037 | 0.021 | 0.0289 | 0.0029 | 0.0055 | 0.002 | 0.0007 | 0.0229 | 0.0065 | 0.0041 |
Mitochondria | 0.0027 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0044 | 0.0001 | 0.0056 | 0.0196 | 0.0236 | 0.0064 | 0.0003 | 0.0001 | 0 | 0.0044 | 0.0001 | 0.0001 |
None | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0015 | 0.0002 | 0.0118 | 0.0003 | 0.0015 | 0.0006 | 0.0014 | 0.0008 | 0.0021 | 0.0007 | 0.0003 | 0.0046 | 0.0002 | 0.0003 |
Nuclear Periphery | 0.0013 | 0.0004 | 0.001 | 0.0021 | 0.0014 | 0.0004 | 0.0232 | 0.0007 | 0.0025 | 0.0014 | 0.0093 | 0.0017 | 0.0028 | 0.0007 | 0.0011 | 0.0081 | 0.0014 | 0.0013 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0017 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 |
Nucleus | 0.0007 | 0.0005 | 0.0013 | 0.0012 | 0.0017 | 0.0006 | 0.0184 | 0.0007 | 0.0035 | 0.0026 | 0.0016 | 0.0018 | 0.0012 | 0.0006 | 0.001 | 0.004 | 0.0013 | 0.0008 |
Peroxisomes | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.007 | 0 | 0.0005 | 0.0039 | 0.002 | 0.0009 | 0.0007 | 0.0001 | 0 | 0.0018 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0065 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0007 | 0.0008 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0009 |
Vacuole | 0.012 | 0.0033 | 0.0206 | 0.0193 | 0.0163 | 0.0053 | 0.024 | 0.005 | 0.0182 | 0.0241 | 0.0079 | 0.0044 | 0.0148 | 0.0052 | 0.012 | 0.0883 | 0.0059 | 0.0028 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0007 | 0.0015 | 0.0015 | 0.0002 | 0.0024 | 0.0001 | 0.001 | 0.0047 | 0.0028 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0097 | 0.0007 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 182.1409 | 203.7252 | 210.7082 | 141.2658 | 175.6892 | 233.0465 | 169.0043 | 160.4982 | 192.9153 | 122.9386 |
Translational Efficiency | 1.4442 | 1.542 | 1.3844 | 2.1667 | 1.3884 | 1.0357 | 1.3338 | 1.6522 | 1.2252 | 1.4879 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
334 | 186 | 2287 | 1532 | 2325 | 1686 | 108 | 1199 | 2659 | 1872 | 2395 | 2731 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1356.22 | 1807.26 | 1941.84 | 2187.24 | 1595.82 | 1716.36 | 2137.20 | 2166.83 | 1565.72 | 1725.39 | 1950.65 | 2178.28 |
Standard Deviation | 361.49 | 408.31 | 313.88 | 353.14 | 249.04 | 280.33 | 298.68 | 416.86 | 277.40 | 296.79 | 315.83 | 382.56 |
Intensity Change Log 2 | — | 0.414213 | 0.517833 | 0.689520 | — | 0.105054 | 0.421424 | 0.441288 | — | 0.255346 | 0.466517 | 0.560652 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001411 | 0.002021 | 0.004138 | 0.004158 | 0.000929 | 0.003678 | 0.016380 | 0.006988 | 0.000990 | 0.003513 | 0.004690 | 0.005401 |
Bud Neck | 0.004262 | 0.008415 | 0.009379 | 0.006764 | 0.003296 | 0.006476 | 0.021347 | 0.008550 | 0.003417 | 0.006668 | 0.009919 | 0.007548 |
Bud Site | 0.061750 | 0.083558 | 0.079822 | 0.064077 | 0.044831 | 0.079499 | 0.102348* | 0.088430 | 0.046957 | 0.079903 | 0.080838 | 0.074768 |
Cell Periphery | 0.016582 | 0.023133 | 0.029570 | 0.078629 | 0.010026 | 0.008777 | 0.100299* | 0.060187 | 0.010850 | 0.010204 | 0.032759 | 0.070533 |
Cytoplasm | 0.712674* | 0.670997* | 0.705777* | 0.696936* | 0.873399* | 0.697823* | 0.340649* | 0.651156* | 0.853211* | 0.695158* | 0.689312* | 0.676837* |
Cytoplasmic Foci | 0.095719 | 0.047919 | 0.009596 | 0.002777 | 0.020414 | 0.065591 | 0.021611 | 0.006099 | 0.029873 | 0.063835 | 0.010137 | 0.004236 |
Eisosomes | 0.000032 | 0.000043 | 0.000054 | 0.000138 | 0.000028 | 0.000044 | 0.000303 | 0.000298 | 0.000029 | 0.000044 | 0.000066 | 0.000208 |
Endoplasmic Reticulum | 0.007037 | 0.003724 | 0.001166 | 0.000365 | 0.005452 | 0.004201 | 0.013190 | 0.000610 | 0.005651 | 0.004154 | 0.001709 | 0.000473 |
Endosome | 0.009694 | 0.008879 | 0.002902 | 0.000524 | 0.001596 | 0.009263 | 0.010311 | 0.003058 | 0.002613 | 0.009224 | 0.003236 | 0.001636 |
Golgi | 0.014010 | 0.025853 | 0.004552 | 0.002183 | 0.006557 | 0.017743 | 0.008921 | 0.005028 | 0.007493 | 0.018549 | 0.004749 | 0.003432 |
Lipid Particles | 0.024179 | 0.080479 | 0.040166 | 0.034025 | 0.013667 | 0.059277 | 0.106260* | 0.032803 | 0.014987 | 0.061383 | 0.043146 | 0.033489 |
Mitochondria | 0.000281 | 0.000030 | 0.000301 | 0.000496 | 0.000291 | 0.000406 | 0.000018 | 0.000774 | 0.000289 | 0.000369 | 0.000288 | 0.000618 |
Mitotic Spindle | 0.004887 | 0.001640 | 0.000577 | 0.000312 | 0.000274 | 0.003993 | 0.000817 | 0.001995 | 0.000854 | 0.003759 | 0.000588 | 0.001051 |
None | 0.000253 | 0.000563 | 0.000220 | 0.000205 | 0.001943 | 0.000763 | 0.000068 | 0.000370 | 0.001731 | 0.000743 | 0.000213 | 0.000277 |
Nuclear Periphery | 0.000061 | 0.000057 | 0.000066 | 0.000138 | 0.000023 | 0.000039 | 0.000227 | 0.000132 | 0.000028 | 0.000041 | 0.000073 | 0.000135 |
Nuclear Periphery Foci | 0.002647 | 0.010387 | 0.002943 | 0.002684 | 0.001827 | 0.004218 | 0.015612 | 0.002871 | 0.001930 | 0.004831 | 0.003514 | 0.002766 |
Nucleolus | 0.000053 | 0.000035 | 0.000103 | 0.000079 | 0.000069 | 0.000199 | 0.000040 | 0.000127 | 0.000067 | 0.000182 | 0.000100 | 0.000100 |
Nucleus | 0.003094 | 0.001830 | 0.003668 | 0.003783 | 0.002701 | 0.003688 | 0.009101 | 0.004635 | 0.002750 | 0.003504 | 0.003913 | 0.004157 |
Peroxisomes | 0.001027 | 0.001076 | 0.001072 | 0.000503 | 0.000736 | 0.002083 | 0.001148 | 0.000847 | 0.000773 | 0.001983 | 0.001076 | 0.000654 |
Vacuole | 0.033697 | 0.026532 | 0.100944 | 0.099722 | 0.009402 | 0.028126 | 0.230391* | 0.120815 | 0.012454 | 0.027967 | 0.106781 | 0.108982 |
Vacuole Periphery | 0.006648 | 0.002833 | 0.002984 | 0.001503 | 0.002538 | 0.004115 | 0.000960 | 0.004226 | 0.003054 | 0.003988 | 0.002893 | 0.002698 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.42 | -6.70 | -6.38 | -4.24 | -0.17 | -4.83 | -2.80 | -9.02 | -3.66 | 1.77 | -4.90 | -8.96 | -12.12 | -3.02 | -1.14 |
Bud Neck | -1.63 | -5.18 | -2.77 | 0.62 | 3.24 | -4.17 | -8.16 | -8.01 | -2.44 | 5.79 | -4.46 | -9.66 | -8.10 | -1.27 | 3.51 |
Bud Site | -1.61 | -2.23 | -0.46 | 1.53 | 3.06 | -7.57 | -3.80 | -7.83 | -1.17 | 1.03 | -7.58 | -8.75 | -7.61 | 1.07 | 1.48 |
Cell Periphery | -1.12 | -3.41 | -12.03 | -8.54 | -11.21 | 1.18 | -5.45 | -12.11 | -12.61 | 2.25 | 0.66 | -10.25 | -19.69 | -20.08 | -11.04 |
Cytoplasm | 1.59 | 0.68 | 1.05 | -1.09 | 0.71 | 20.88 | 21.43 | 24.20 | 4.28 | -11.69 | 19.47 | 23.65 | 26.23 | 2.08 | 1.47 |
Cytoplasmic Foci | 4.62 | 10.73 | 11.63 | 6.93 | 11.10 | -14.74 | -1.01 | 11.39 | 19.24 | 5.39 | -11.35 | 12.99 | 17.73 | 21.29 | 9.04 |
Eisosomes | -0.85 | -4.26 | -4.48 | -3.68 | -3.54 | -3.61 | -5.81 | -3.53 | -3.31 | 0.00 | -3.68 | -8.40 | -5.05 | -4.60 | -3.96 |
Endoplasmic Reticulum | 1.13 | 2.30 | 2.62 | 2.25 | 4.88 | 1.43 | -1.44 | 6.97 | 5.77 | 2.61 | 1.76 | 5.55 | 7.60 | 6.42 | 4.65 |
Endosome | 0.30 | 4.39 | 6.03 | 3.63 | 9.95 | -9.11 | -4.34 | -2.09 | 6.09 | 3.51 | -8.10 | -2.17 | 2.83 | 9.18 | 4.80 |
Golgi | -2.95 | 5.15 | 6.42 | 6.50 | 3.83 | -9.48 | -1.38 | 1.72 | 8.64 | 2.24 | -9.66 | 4.96 | 6.60 | 12.84 | 2.23 |
Lipid Particles | -4.58 | -3.44 | -2.14 | 3.96 | 2.24 | -11.58 | -7.03 | -7.43 | 5.92 | 5.42 | -12.34 | -12.18 | -9.88 | 7.13 | 3.83 |
Mitochondria | 1.51 | -0.05 | -0.48 | -1.21 | -0.48 | -0.83 | 6.58 | -0.90 | -0.63 | -1.49 | -0.62 | 0.10 | -0.99 | -0.68 | -0.98 |
Mitotic Spindle | 1.19 | 1.56 | 1.68 | 5.65 | 1.26 | -4.72 | -2.56 | -2.08 | 1.65 | -1.24 | -3.76 | 0.51 | -0.52 | 3.31 | -1.06 |
None | -2.30 | 0.76 | 1.00 | 2.69 | 0.29 | 4.34 | 7.85 | 5.75 | 2.04 | -2.22 | 4.11 | 7.26 | 6.69 | 3.40 | -1.11 |
Nuclear Periphery | 0.24 | -0.49 | -4.50 | -6.33 | -6.71 | -5.88 | -4.15 | -7.50 | -6.17 | 2.24 | -4.42 | -8.73 | -12.72 | -10.80 | -5.88 |
Nuclear Periphery Foci | -3.78 | -1.51 | -0.45 | 3.74 | 1.54 | -6.31 | -7.89 | -4.19 | 3.00 | 7.20 | -7.29 | -6.83 | -4.97 | 5.03 | 3.29 |
Nucleolus | 1.63 | -4.69 | -2.56 | -8.51 | 4.50 | -1.08 | 2.14 | -6.08 | 0.58 | -6.17 | -1.07 | -6.52 | -6.14 | 0.75 | -0.23 |
Nucleus | 2.14 | -1.82 | -1.90 | -7.87 | -0.16 | -2.98 | -2.25 | -5.50 | -2.94 | 1.51 | -2.55 | -5.57 | -7.05 | -2.93 | -0.94 |
Peroxisomes | -0.22 | -0.16 | 4.50 | 2.65 | 7.83 | -5.91 | -1.03 | -0.76 | 5.27 | 0.75 | -5.86 | -3.32 | 1.77 | 6.58 | 5.33 |
Vacuole | 1.11 | -13.52 | -12.66 | -13.42 | 0.62 | -11.24 | -12.61 | -25.47 | -20.32 | 5.66 | -9.55 | -30.18 | -34.97 | -26.60 | -1.03 |
Vacuole Periphery | 1.56 | 1.64 | 2.30 | 1.35 | 4.03 | -2.40 | 5.36 | -1.39 | 0.11 | -3.10 | -1.37 | 0.46 | 0.87 | 2.02 | 0.57 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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