Standard name
Human Ortholog
Description Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0.06 0 0 0 0.05
Bud 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.5 0.52 0.5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.16 0.11 0.06 0.06 0.05 0.06 0.06
Golgi 0 0.25 0.07 0.18 0 0 0 0 0 0 0 0 0.5 0.54 0.68 0 0 0 0.09 0.13 0.09 0.09 0.13 0.11
Mitochondria 0.81 0.69 0.91 0.9 0.88 0.9 0.95 0.92 0.95 0.91 0.97 0.96 0.5 0.45 0.38 0.11 0 0.14 0.61 0.56 0.71 0.67 0.54 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 0.05 0.05 0.09 0.07 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0 0 0.06 0.06 0.06 0.1 0.08 0.07 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.24 0.09 0.08 0.09 0.05 0.09 0.06 0.06 0.08 0 0.05 0.31 0.32 0.21 0.2 0.19 0.11 0 0.1 0 0.06 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 9 3 3 0 0 0 0 0 0 0 0 10 8 10 2 7 1 3 12 8 4 8 14
Bud 5 0 3 7 11 9 8 13 13 12 16 12 1 2 3 1 0 1 1 3 0 1 6 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 3
Bud Site 0 1 0 0 0 4 4 6 4 12 5 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 20 4 5 1 2 4 2 0 2 3 3 7 5 5 5 6 2 0 0 0 0 0 0
Cytoplasm 7 8 6 8 13 10 14 11 5 11 5 7 5 4 7 23 35 22 3 3 0 0 0 0
Endoplasmic Reticulum 1 2 4 4 3 9 1 1 1 2 2 5 3 5 8 3 3 1 0 1 1 1 0 3
Endosome 5 5 7 4 7 1 2 2 3 5 0 1 4 4 0 5 7 7 12 12 21 8 15 17
Golgi 1 49 22 46 5 3 0 0 0 0 0 0 117 134 189 2 3 1 10 29 30 17 33 33
Mitochondria 77 134 286 229 292 337 532 481 495 475 327 495 116 113 105 5 2 6 68 121 244 124 140 167
Nucleus 2 1 0 0 3 7 9 18 5 9 7 0 0 1 1 0 0 0 0 0 0 1 1 0
Nuclear Periphery 1 2 9 5 7 21 27 27 47 34 14 40 2 3 5 0 0 1 0 1 3 0 2 5
Nucleolus 10 3 11 6 21 21 33 51 44 39 17 11 7 8 7 1 0 3 0 1 0 0 2 0
Peroxisomes 0 0 8 3 1 3 1 4 1 4 0 4 1 1 1 0 1 2 2 3 15 7 8 5
SpindlePole 0 0 4 0 3 6 5 13 4 15 2 3 0 2 0 1 0 1 1 1 3 2 2 1
Vac/Vac Membrane 9 47 28 20 31 19 48 33 29 44 15 25 72 80 59 9 13 5 2 22 6 12 29 31
Unique Cell Count 95 194 313 255 332 375 562 524 522 523 338 514 233 249 278 46 67 44 112 217 343 187 258 299
Labelled Cell Count 118 281 395 340 398 452 688 662 651 664 413 613 345 370 400 57 77 53 112 217 343 187 258 299


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 9.9 7.4 8.0 6.9 5.7 5.0 5.0 4.8 4.4 4.7 4.7 15.4 17.1 18.1 7.5 7.9 7.8 9.3 9.4 9.7
Std Deviation (1e-4) 1.8 2.2 1.6 1.9 1.6 1.3 1.3 1.3 1.3 1.2 1.3 1.1 3.5 4.3 4.8 1.5 2.1 1.8 1.9 2.0 2.2
Intensity Change (Log2) – – – 0.12 -0.1 -0.38 -0.56 -0.57 -0.61 -0.75 -0.66 -0.66 1.06 1.21 1.29 0.02 0.1 0.08 0.33 0.35 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.5 0 2.2 0 0 0
Bud 0 2.1 1.4 0 1.6 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.5 0.6 0.5 0.2 0 0.2 -0.4 0 0 0 0.5 0 12.0 0
Endoplasmic Reticulum 0 0 1.1 0 0 0 0 0 0 0 0 1.4 0 0 0
Endosome 0 -0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 4.0 -3.5 -4.3 -6.4 -6.2 0 0 0 0 11.5 12.3 15.4 0 0 0
Mitochondria -0.6 -1.4 -0.7 1.9 0.2 0 0 0 0 -10.9 -11.9 -13.7 -13.0 -15.3 -12.5
Nucleus 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 -0.6 1.7 1.4 1.6 0 0 0 0 0 -1.4 -0.9 0 0 0
Nucleolus -0.8 1.6 1.3 1.5 3.3 0 0 0 0 -0.3 -0.2 -0.7 0 0 0
Peroxisomes 0 0 -1.8 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.2 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 0.2 -2.0 -0.2 -1.4 -1.9 -0.3 -2.3 -2.3 6.6 6.9 4.2 0 2.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9403 5.9058 5.0773 4.5779 5.8 5.2573 7.2479 7.7793 7.0969 6.9343 7.0114 7.6603 5.1675 6.1312 5.3231 5.1514 5.8434 5.7321
Actin 0.0165 0.0241 0.0199 0.0052 0.012 0.0062 0.0296 0.0417 0.0278 0.0295 0.0106 0.0201 0.0095 0.0197 0.0191 0.0133 0.0114 0.0079
Bud 0.0036 0.0146 0.0077 0.009 0.0084 0.0027 0.0034 0.0079 0.0061 0.009 0.01 0.003 0.0032 0.0064 0.0074 0.005 0.007 0.0038
Bud Neck 0.0015 0.0017 0.0032 0.0049 0.0014 0.0039 0.0014 0.0013 0.0033 0.0013 0.0011 0.0021 0.0015 0.0012 0.0012 0.0014 0.0013 0.0032
Bud Periphery 0.0062 0.0157 0.013 0.0292 0.0184 0.0042 0.0061 0.0116 0.0109 0.0205 0.0268 0.0058 0.0048 0.0056 0.0119 0.0076 0.0146 0.0071
Bud Site 0.0087 0.0467 0.0221 0.0061 0.0054 0.0024 0.0083 0.0423 0.0128 0.0073 0.0031 0.0021 0.0088 0.0277 0.0116 0.0037 0.0027 0.0028
Cell Periphery 0.0016 0.0018 0.0019 0.003 0.0009 0.0006 0.0014 0.0016 0.0064 0.0023 0.0007 0.0012 0.0021 0.001 0.0009 0.0004 0.0004 0.0006
Cytoplasm 0.0451 0.1025 0.0713 0.0437 0.1099 0.0649 0.0479 0.1007 0.0568 0.0585 0.0583 0.0421 0.0445 0.1172 0.0634 0.018 0.0658 0.0495
Cytoplasmic Foci 0.0626 0.087 0.0733 0.0687 0.0571 0.0719 0.0652 0.0778 0.0789 0.0652 0.0693 0.0611 0.0673 0.1023 0.0657 0.042 0.0781 0.0772
Eisosomes 0.0003 0.0003 0.0004 0.0002 0.0004 0.0002 0.0004 0.0004 0.0007 0.0005 0.0003 0.0006 0.0005 0.0002 0.0003 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0105 0.0111 0.01 0.0087 0.0127 0.0089 0.0112 0.0052 0.0029 0.0085 0.0069 0.005 0.0141 0.0058 0.0052 0.0019 0.003 0.0089
Endosome 0.2537 0.2015 0.1768 0.2135 0.2024 0.2255 0.2091 0.1421 0.1154 0.1484 0.1837 0.1485 0.2389 0.2159 0.1668 0.1791 0.2073 0.1891
Golgi 0.1244 0.1217 0.1245 0.1025 0.1336 0.152 0.1674 0.162 0.1289 0.0629 0.1552 0.1776 0.1316 0.1602 0.1287 0.1139 0.1703 0.1622
Lipid Particles 0.0054 0.0029 0.0045 0.0032 0.0074 0.003 0.0053 0.0039 0.0036 0.0012 0.0013 0.0022 0.0152 0.0023 0.0019 0.0031 0.0015 0.0033
Mitochondria 0.4135 0.3131 0.421 0.4568 0.3748 0.4072 0.4056 0.3732 0.5068 0.523 0.4411 0.5051 0.4053 0.3005 0.4827 0.5751 0.4034 0.4583
None 0.0022 0.0013 0.0027 0.0008 0.009 0.0086 0.0013 0.0034 0.0044 0.0018 0.0011 0.001 0.0031 0.0015 0.0024 0.0008 0.0009 0.0012
Nuclear Periphery 0.0033 0.0028 0.0022 0.003 0.0031 0.0027 0.0033 0.0008 0.0005 0.0013 0.0006 0.0008 0.0084 0.0009 0.0012 0.0006 0.0004 0.0011
Nucleolus 0.0004 0.0006 0.0005 0.0002 0.0013 0.0004 0.0002 0.0003 0.0003 0.0002 0.0001 0.0001 0.0008 0.0002 0.0002 0.0002 0.0001 0.0002
Nucleus 0.0012 0.0033 0.0022 0.001 0.0024 0.0025 0.0008 0.001 0.0007 0.0011 0.0006 0.0006 0.0014 0.0012 0.0009 0.0007 0.0007 0.0006
Peroxisomes 0.0111 0.0118 0.0182 0.011 0.0069 0.0077 0.0138 0.013 0.0201 0.0286 0.0062 0.0105 0.0092 0.0144 0.0109 0.0079 0.01 0.0092
Punctate Nuclear 0.0008 0.0019 0.0006 0.0002 0.0082 0.0019 0.0003 0.0003 0.0003 0.0003 0.0002 0.0002 0.0024 0.0003 0.0003 0.0002 0.0003 0.0005
Vacuole 0.0169 0.0253 0.0166 0.0174 0.0158 0.0129 0.0106 0.0059 0.0089 0.021 0.016 0.0055 0.0168 0.0101 0.0104 0.0163 0.0153 0.0072
Vacuole Periphery 0.0107 0.0085 0.0074 0.0116 0.0087 0.0098 0.0073 0.0036 0.0036 0.0077 0.0071 0.0049 0.0106 0.0052 0.0067 0.0086 0.0054 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.1181 29.1062 23.9978 28.0059 14.0716 28.9257 53.4086 68.784 44.3528 34.2358
Translational Efficiency 1.0369 1.167 1.1054 0.7527 0.8408 1.2492 0.9983 0.6398 1.0369 0.8734

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mrs4

Mrs4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrs4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available