Standard name
Human Ortholog
Description Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.05 0 0.07 0 0 0.05 0 0 0 0.07 0.15 0.15 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.09 0.08 0.07 0 0.06 0.08 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.22 0 0 0.25 0.05 0.06 0.08 0.35 0.14 0.21 0.23 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.25 0.21 0.25 0 0.15 0.12 0.24 0.15 0.1 0.14 0.14 0.11 0.14 0.11 0.14 0.25 0.22 0.3 0.22 0.16 0.16 0.12 0.11 0.11
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.83 0.74 0.72 0.85 0.75 0.76 0.63 0.68 0.7 0.74 0.79 0.8 0.83 0.74 0.74 0.77 0.77 0.73 0.67 0.61 0.58 0.67 0.64 0.65
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.32 0.47 0.31 0.4 0.34 0.44 0.27 0.39 0.33 0.35 0.38 0.34 0.22 0.06 0.1 0.13 0.05 0.07 0.14 0.12 0.13 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud 0 0 0 0 4 0 0 0 0 3 2 3 0 2 0 0 0 0 3 1 0 4 5 9
Bud Neck 3 0 3 5 2 9 4 6 4 5 0 4 15 23 3 2 2 0 1 1 1 2 3 8
Bud Site 0 0 0 1 4 3 4 2 4 4 1 2 0 1 0 0 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 3 6 6 5 4 11 8 7 8 5 3 2 37 95 101 22 16 11 0 3 2 1 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 4 3 0 0 0 0 0 3
Endosome 4 0 1 1 0 14 15 12 3 12 14 5 16 33 50 10 13 6 3 8 3 12 15 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 3 0 0 2 2
Mitochondria 10 43 1 0 23 8 11 14 58 29 35 31 1 1 1 31 17 7 1 6 0 1 2 4
Nucleus 73 40 24 4 14 19 44 27 17 28 23 15 70 66 94 138 91 111 66 36 19 52 45 49
Nuclear Periphery 2 10 1 0 0 0 0 1 0 2 0 1 0 2 0 2 6 0 0 2 0 0 3 5
Nucleolus 242 143 70 81 68 124 116 120 116 149 130 108 414 461 497 428 316 266 202 137 71 285 257 302
Peroxisomes 1 0 0 0 0 0 1 1 0 1 1 0 1 0 2 2 2 1 0 0 0 0 0 2
SpindlePole 9 6 31 45 28 66 62 77 44 77 54 47 190 212 145 33 43 46 16 15 17 50 53 43
Vac/Vac Membrane 2 3 0 1 0 2 1 5 1 0 0 0 2 5 5 8 10 7 5 4 1 7 6 11
Unique Cell Count 290 194 97 95 91 163 184 176 165 200 165 135 500 620 673 554 410 364 304 225 123 425 400 469
Labelled Cell Count 349 251 137 143 147 256 266 272 255 315 263 218 746 902 900 683 521 462 304 225 123 425 400 469


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 8.5 8.5 8.1 5.2 7.1 6.6 7.0 5.2 5.9 5.3 5.0 8.7 8.6 8.2 11.3 13.4 12.9 9.4 9.7 10.2
Std Deviation (1e-4) 8.7 2.2 1.8 2.3 1.2 2.3 2.1 2.2 1.8 1.5 1.5 0.9 2.2 2.3 2.5 4.4 4.4 4.4 1.9 2.0 2.0
Intensity Change (Log2) – – – -0.08 -0.73 -0.27 -0.38 -0.29 -0.71 -0.54 -0.69 -0.79 0.02 0.0 -0.06 0.4 0.65 0.6 0.14 0.18 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 0 0.2 0 0 -0.5 0 0 0 0.4 2.4 2.3 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 2.5 2.4 0 0 0 2.5 0 0 0 2.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.0 0 0 2.4 6.4 3.6 4.6 4.8 0 0 0 0 0 0
Nucleus -4.0 -1.6 -2.7 -0.2 -1.9 -3.1 -2.3 -2.2 -2.7 -2.7 -3.9 -2.8 0 -0.5 1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.2 0.4 0.7 -1.5 -0.7 -0.3 0.4 1.2 1.4 2.4 0.5 0.4 1.1 1.0 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.2 -0.2 1.4 0.3 1.9 -0.9 1.1 0.1 0.5 1.1 0.4 -2.3 -7.9 -5.4 -4.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0552 3.7198 2.9169 3.229 1.9247 2.7691 10.0787 9.227 9.5666 8.9211 8.8898 9.9257 6.5547 6.9655 6.5782 6.3609 6.5517 6.5072
Actin 0.1526 0.0004 0.0005 0.0037 0 0.0034 0.0085 0 0.0016 0 0 0.0004 0.0361 0 0.0041 0.0001 0.0259 0
Bud 0.0009 0.0002 0.0002 0.0004 0 0.0001 0.0002 0 0 0 0 0 0.0005 0.0001 0.0002 0.0002 0.0001 0
Bud Neck 0.0026 0.0011 0.0009 0.0013 0.0002 0.0005 0.0017 0.0009 0.0006 0.0006 0.0004 0.0004 0.0134 0.0007 0.0022 0.0005 0.0004 0.0006
Bud Periphery 0.0017 0.0003 0.0004 0.0005 0.0002 0.0002 0.0003 0.0001 0.0001 0 0 0 0.0011 0.0001 0.0004 0.0003 0.0002 0
Bud Site 0.0047 0.0018 0.0008 0.0006 0.0002 0.0002 0.0014 0.0007 0.0002 0.0001 0.0001 0.0001 0.0074 0.0005 0.0073 0.0008 0.0004 0.0001
Cell Periphery 0.0007 0.0003 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0008 0.0003 0.0005 0.0005 0.0001 0.0001
Cytoplasm 0.0075 0 0.0001 0.0003 0 0.0001 0.002 0.0001 0.0007 0 0 0.0001 0.0198 0 0.0009 0.001 0.0013 0
Cytoplasmic Foci 0.0481 0.0004 0.0066 0.01 0.0005 0.0032 0.0037 0.0005 0.0008 0.0003 0.0003 0.0004 0.0093 0.003 0.0061 0.0078 0.0098 0.0001
Eisosomes 0.0008 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0075 0 0.0001 0.0001 0 0.0003 0.0016 0 0.0006 0 0 0.0003 0.006 0 0.0015 0.0003 0.0012 0
Endosome 0.0585 0.0003 0.0004 0.0113 0.0001 0.0021 0.0049 0.0002 0.0008 0.0001 0 0.0019 0.0164 0.0003 0.0074 0.0015 0.0081 0
Golgi 0.0248 0.0002 0.0007 0.0139 0.0001 0.0046 0.0015 0.0001 0.0002 0.0001 0 0.0004 0.0104 0.0001 0.0015 0.0003 0.005 0
Lipid Particles 0.0608 0.0011 0.0077 0.0028 0.0118 0.0249 0.0045 0.0012 0.0014 0.0015 0.0011 0.0028 0.0208 0.0022 0.0114 0.0076 0.0071 0.0005
Mitochondria 0.008 0.0037 0.0039 0.009 0.0299 0.0039 0.0053 0.0001 0.0001 0 0.0001 0.0011 0.0198 0.0001 0.0033 0.0031 0.0008 0
None 0.0079 0 0.0002 0.0001 0.0001 0.0001 0.0048 0.0001 0.0005 0 0 0.0001 0.0159 0.0001 0.0019 0.0015 0.0011 0
Nuclear Periphery 0.0108 0.0008 0.002 0.0002 0.0001 0.0023 0.0095 0.0039 0.0007 0.0002 0.0025 0.003 0.0187 0.0001 0.0032 0.0018 0.0023 0
Nucleolus 0.49 0.8182 0.8306 0.7968 0.86 0.8187 0.7942 0.7078 0.8252 0.7433 0.7305 0.8291 0.6339 0.8335 0.8361 0.8202 0.7963 0.8727
Nucleus 0.0706 0.157 0.1197 0.1366 0.0889 0.1051 0.1315 0.2233 0.1298 0.2171 0.1663 0.1269 0.1115 0.1516 0.103 0.1392 0.1071 0.1181
Peroxisomes 0.019 0.0002 0.0108 0.0068 0.0012 0.0032 0.0036 0.0001 0.0001 0 0 0.0001 0.0021 0.0001 0.0031 0.0005 0.0116 0
Punctate Nuclear 0.015 0.0122 0.0133 0.0048 0.0018 0.0255 0.0178 0.0604 0.0344 0.0364 0.0983 0.0323 0.0479 0.0036 0.0041 0.0085 0.0207 0.0074
Vacuole 0.0057 0.0016 0.0006 0.0005 0.0015 0.0004 0.0015 0.0004 0.0015 0.0001 0.0001 0.0003 0.0053 0.0034 0.0013 0.0025 0.0003 0.0002
Vacuole Periphery 0.002 0.0002 0.0003 0.0002 0.0028 0.001 0.0013 0.0001 0.0002 0 0 0.0002 0.0028 0.0003 0.0005 0.0017 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7182 11.9561 20.4054 18.0839 19.0748 2.6734 17.5287 16.7262 11.7122 23.1095
Translational Efficiency 2.6794 3.8333 1.7232 1.3065 2.0311 11.5058 1.8872 1.3594 1.3475 1.5594

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
91 1193 3016 984 1585 1805 2626 1303 1676 2998 5642 2287

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 963.64 951.47 1104.04 1121.52 865.49 1007.37 1140.76 1170.55 870.82 985.13 1121.13 1149.45
Standard Deviation 179.14 184.16 182.15 244.89 144.08 166.29 193.94 242.80 147.88 175.77 188.62 244.91
Intensity Change Log 2 — -0.018336 0.196226 0.218889 — 0.219005 0.398406 0.435598 — 0.098842 0.295429 0.325493

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000635 0.000349 0.000267 0.000769 0.000263 0.000303 0.000254 0.000468 0.000283 0.000321 0.000261 0.000598
Bud Neck 0.009048 0.004201 0.007604 0.021431 0.003395 0.004424 0.005540 0.011699 0.003702 0.004335 0.006643 0.015886
Bud Site 0.001739 0.000893 0.000802 0.005266 0.000692 0.001023 0.000563 0.002824 0.000749 0.000971 0.000691 0.003875
Cell Periphery 0.000116 0.000058 0.000030 0.000174 0.000114 0.000077 0.000055 0.000166 0.000114 0.000069 0.000042 0.000170
Cytoplasm 0.005715 0.000630 0.000161 0.002764 0.001592 0.000691 0.000115 0.001195 0.001816 0.000667 0.000139 0.001870
Cytoplasmic Foci 0.008697 0.001898 0.000153 0.002152 0.000873 0.001052 0.000172 0.001724 0.001298 0.001388 0.000161 0.001908
Eisosomes 0.000110 0.000115 0.000072 0.000099 0.000109 0.000116 0.000093 0.000126 0.000109 0.000115 0.000082 0.000115
Endoplasmic Reticulum 0.004385 0.001324 0.000395 0.001227 0.001610 0.001549 0.000393 0.000615 0.001760 0.001459 0.000394 0.000879
Endosome 0.008007 0.001468 0.000167 0.003520 0.000604 0.001126 0.000179 0.001338 0.001006 0.001262 0.000173 0.002277
Golgi 0.002146 0.001042 0.000325 0.001345 0.000556 0.000937 0.000212 0.000955 0.000643 0.000979 0.000272 0.001123
Lipid Particles 0.008022 0.000632 0.000019 0.000559 0.000211 0.000908 0.000030 0.000143 0.000635 0.000798 0.000024 0.000322
Mitochondria 0.003958 0.001148 0.001258 0.005229 0.000777 0.002518 0.001185 0.003249 0.000949 0.001973 0.001224 0.004101
Mitotic Spindle 0.039841 0.004075 0.013799 0.019143 0.004382 0.006105 0.005725 0.006931 0.006307 0.005297 0.010041 0.012185
None 0.000792 0.001156 0.000357 0.004776 0.002421 0.001126 0.000551 0.001785 0.002332 0.001138 0.000447 0.003072
Nuclear Periphery 0.000944 0.000364 0.000554 0.001924 0.000520 0.000453 0.000499 0.000923 0.000543 0.000417 0.000528 0.001354
Nuclear Periphery Foci 0.008272 0.002732 0.000490 0.001245 0.005614 0.002322 0.000537 0.001169 0.005759 0.002485 0.000512 0.001202
Nucleolus 0.572080* 0.613661* 0.607341* 0.532214* 0.590847* 0.558650* 0.635055* 0.622446* 0.589828* 0.580541* 0.620240* 0.583623*
Nucleus 0.297339* 0.351295* 0.359318* 0.375603* 0.377294* 0.407474* 0.341542* 0.327532* 0.372953* 0.385119* 0.351045* 0.348215*
Peroxisomes 0.016218 0.009594 0.005257 0.005653 0.006949 0.006610 0.005388 0.005444 0.007453 0.007797 0.005318 0.005534
Vacuole 0.009532 0.002023 0.000628 0.008691 0.000649 0.001339 0.000725 0.004574 0.001131 0.001611 0.000673 0.006345
Vacuole Periphery 0.002404 0.001341 0.001003 0.006214 0.000527 0.001199 0.001186 0.004692 0.000629 0.001255 0.001088 0.005347

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.13 2.65 -1.45 -6.01 -7.18 -1.06 0.33 -7.75 -4.75 -8.91 -1.19 0.87 -8.79 -7.54 -9.75
Bud Neck 1.75 0.18 -5.89 -14.50 -11.80 -2.97 -6.44 -9.57 -8.49 -6.94 -2.02 -10.25 -16.84 -16.20 -12.91
Bud Site 2.08 2.00 -4.39 -5.29 -5.26 -1.56 0.39 -3.92 -3.26 -4.06 -1.40 -0.52 -6.59 -6.16 -6.56
Cell Periphery 1.48 2.22 -1.40 -6.03 -8.31 1.21 1.76 -1.39 -3.16 -3.55 1.56 2.49 -1.81 -5.50 -6.81
Cytoplasm 2.00 2.19 0.54 -4.52 -5.21 1.62 3.14 0.34 -1.25 -2.75 2.28 3.59 -1.24 -4.14 -5.77
Cytoplasmic Foci 1.60 2.09 1.61 0.03 -4.65 -0.48 4.00 -1.96 -1.56 -3.71 -0.34 4.17 -1.47 -1.22 -5.66
Eisosomes -0.26 2.64 0.98 2.18 -3.55 -0.80 2.53 -1.19 -0.71 -2.93 -0.82 4.71 -0.20 0.52 -4.49
Endoplasmic Reticulum 2.30 2.96 2.22 -0.53 -6.15 0.49 10.22 8.25 6.51 -2.71 2.16 10.82 5.50 4.13 -6.69
Endosome 1.39 1.68 1.02 -1.98 -4.08 -2.17 3.98 -1.67 -0.45 -2.85 -0.67 3.10 -2.27 -1.93 -4.98
Golgi 1.24 2.21 1.01 -0.44 -2.87 -1.00 2.98 -0.69 -0.00 -1.94 -1.10 3.10 -1.15 -0.27 -3.14
Lipid Particles 2.07 2.25 2.09 0.27 -3.11 -1.92 4.44 1.09 2.10 -2.56 -0.53 3.05 1.46 1.88 -3.80
Mitochondria 1.60 1.48 -0.66 -5.30 -5.03 -3.36 -2.85 -4.15 -1.02 -3.34 -3.11 -2.60 -6.50 -3.77 -5.83
Mitotic Spindle 2.40 1.76 1.03 -6.08 -3.08 -1.12 -1.12 -2.35 -1.42 -1.57 0.97 -2.77 -4.50 -5.73 -2.92
None -1.43 2.24 -2.89 -2.61 -3.26 2.59 3.67 0.89 -1.21 -2.25 2.57 4.11 -0.78 -2.86 -4.00
Nuclear Periphery 2.41 1.64 -2.36 -4.28 -3.80 0.36 0.06 -1.85 -2.22 -1.72 0.78 -0.01 -3.63 -4.66 -3.97
Nuclear Periphery Foci 2.57 3.51 3.18 2.73 -2.88 4.16 6.45 5.20 2.24 -1.34 4.40 7.13 5.95 3.44 -2.46
Nucleolus -1.49 -1.27 1.86 9.36 9.42 4.08 -5.89 -2.31 -5.64 2.30 1.25 -4.61 2.85 2.04 7.52
Nucleus -2.04 -2.35 -3.27 -3.17 -2.74 -3.94 4.85 5.00 8.43 1.18 -1.81 3.52 2.26 4.28 -0.65
Peroxisomes 2.33 3.98 3.78 5.50 -1.10 0.68 4.06 3.54 2.47 -0.21 -0.74 5.64 4.46 5.60 -1.00
Vacuole 2.30 2.70 -0.49 -5.46 -6.34 -3.31 -1.08 -5.19 -4.26 -4.99 -1.63 1.70 -7.42 -6.91 -8.00
Vacuole Periphery 1.54 2.11 -2.85 -4.37 -4.80 -2.98 -3.44 -6.05 -4.82 -4.86 -3.54 -3.56 -7.86 -6.50 -6.88
Standard name
Human Ortholog
Description Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit
Localization
Cell Percentages nucleolus (70%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Utp30

Utp30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available