Standard name
Human Ortholog
Description TFIIE small subunit; involved in RNA polymerase II transcription initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.22 0 0 0 0 0.08 0 0.05 0.08 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.8 0.9 0.89 0.91 0.89 0.81 0.88 0.82 0.84 0.84 0.85 0.92 0.93 0.87 0.8 0.84 0.88 0.8 0.92 0.85 0.82 0.83 0.82
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.23 0.16 0.12 0 0.1 0.17 0.16 0.25 0.21 0.34 0.18 0.08 0.07 0.05 0.09 0.12 0.11 0.11 0 0.1 0.08 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 0 1 2 1 0 1 1 0 0 0 0 0 0 1 0 1 1 5 5
Bud Neck 0 0 0 0 0 0 0 2 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 1 0 0 0 0 1 7 5 7 2 7 7 0 1 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 0 4 21 13 14 8 6 4 6 6 13 15 57 14 1 5 0 0 0 1 1 0
Endoplasmic Reticulum 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 1 0 0 0 2 0 0
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 1 2 4 1 0 0 1 0 0 2 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 1
Mitochondria 10 18 0 1 0 6 35 11 14 21 29 19 0 0 0 6 1 8 1 0 0 1 3 0
Nucleus 130 66 28 88 310 251 370 369 225 231 223 266 316 511 580 173 42 177 130 81 67 263 422 549
Nuclear Periphery 0 0 0 0 0 0 5 2 1 1 1 0 0 0 0 0 0 0 0 0 0 1 0 3
Nucleolus 44 19 5 12 12 27 76 69 69 58 91 56 28 39 32 20 6 23 18 2 7 24 39 63
Peroxisomes 1 0 0 0 0 0 1 0 0 0 0 0 0 1 4 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 1 1 0 1 0 1 0 6 11 1 0 0 0 0 1 2 1 1
Vac/Vac Membrane 0 3 1 0 0 0 2 2 1 0 0 3 4 11 6 15 1 1 5 2 0 10 23 32
Unique Cell Count 154 82 31 99 342 281 455 420 276 274 265 314 345 552 666 217 50 201 163 89 79 320 507 668
Labelled Cell Count 192 108 34 105 344 298 512 473 325 321 358 360 362 587 694 232 51 215 163 89 79 320 507 668


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 7.7 9.1 9.5 10.0 8.4 7.5 7.8 7.1 7.1 6.5 7.2 12.9 12.5 12.0 14.4 15.3 15.8 10.5 10.7 10.9
Std Deviation (1e-4) 1.8 2.3 1.4 3.1 2.1 1.9 1.8 1.8 1.7 1.6 1.5 1.6 2.8 2.9 3.2 4.6 4.6 4.4 2.3 2.4 2.1
Intensity Change (Log2) – – – 0.06 0.13 -0.12 -0.28 -0.22 -0.35 -0.35 -0.5 -0.34 0.5 0.45 0.4 0.66 0.75 0.79 0.2 0.24 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.2 0.1 -0.2 -1.3 -0.4 -1.2 -0.9 -0.9 -0.8 0.2 0.5 -0.5 -1.4 -0.8 -0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.1 0 1.1 0.7 2.0 0.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6044 5.753 5.5951 4.4698 4.9259 5.1935 5.2628 6.3543 6.1017 5.3356 5.679 6.4013 5.0308 5.8433 5.3054 4.6674 7.5592 5.1505
Actin 0.0069 0.0001 0.0014 0 0.0092 0.0001 0.0148 0 0.0012 0 0.0021 0 0.0285 0 0.0125 0.0001 0.0003 0.0035
Bud 0.0004 0.0001 0.0006 0 0.0012 0.0001 0.0018 0.0001 0.0002 0 0.0017 0.0001 0.0007 0 0.0004 0.0002 0.0012 0.0002
Bud Neck 0.0011 0.0004 0.0006 0.0004 0.0011 0.001 0.0008 0.0005 0.0002 0.0005 0.0034 0.0012 0.0019 0.0001 0.0004 0.0007 0.0034 0.0008
Bud Periphery 0.0004 0.0001 0.0007 0 0.0026 0.0002 0.0035 0.0001 0.0003 0.0001 0.0022 0.0004 0.0021 0.0001 0.0004 0.0003 0.0086 0.0005
Bud Site 0.0112 0.0004 0.0032 0.0001 0.0016 0.0003 0.0084 0.0014 0.0004 0.0001 0.0028 0.0002 0.0028 0.0001 0.0025 0.0004 0.0024 0.0003
Cell Periphery 0.0003 0.0001 0.0004 0 0.0004 0.0002 0.0006 0.0001 0.0001 0 0.0009 0.0003 0.0012 0.0001 0.0001 0.0002 0.0061 0.0002
Cytoplasm 0.0219 0.0091 0.01 0 0.0002 0.0016 0.0136 0.0001 0.0021 0 0.0214 0 0.0245 0.0007 0.0009 0.0069 0.0001 0.0004
Cytoplasmic Foci 0.0645 0.0022 0.0131 0 0.0009 0.0053 0.012 0 0.0019 0 0.0088 0 0.0477 0 0.0159 0.0002 0.0005 0.0041
Eisosomes 0.0002 0 0 0 0.0008 0 0.0005 0 0 0 0.0001 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0061 0.0016 0.0015 0 0.0012 0.0012 0.0051 0 0.0007 0 0.0033 0 0.0071 0.0001 0.0005 0.0002 0.0003 0.0008
Endosome 0.0409 0.0031 0.0251 0 0.0066 0.0037 0.0378 0 0.0074 0 0.026 0.0001 0.0901 0.0004 0.0086 0.0006 0.0016 0.0391
Golgi 0.009 0.0001 0.0068 0 0.0165 0.0004 0.0092 0 0.0025 0 0.0025 0 0.016 0 0.0149 0 0.0004 0.0187
Lipid Particles 0.0645 0.0008 0.0174 0 0.0411 0.0048 0.0288 0 0.0155 0 0.0044 0.0001 0.0676 0 0.0086 0.0001 0.0054 0.0111
Mitochondria 0.0358 0.0002 0.0023 0.0002 0.0345 0.0002 0.0324 0.0002 0.0099 0.0001 0.0068 0.0001 0.0042 0.0003 0.0035 0.0009 0.0149 0.0069
None 0.0041 0.0031 0.0004 0 0.0007 0.0006 0.0082 0 0.0007 0 0.002 0 0.0144 0.0001 0.0003 0.0326 0.0003 0.0001
Nuclear Periphery 0.043 0.007 0.0074 0.003 0.0071 0.0063 0.0588 0.0038 0.0179 0.0012 0.0588 0.0002 0.026 0.0093 0.0055 0.0051 0.0013 0.0018
Nucleolus 0.1006 0.1503 0.1406 0.2203 0.3867 0.4821 0.0959 0.0698 0.0992 0.1984 0.3852 0.433 0.1114 0.0635 0.1101 0.1981 0.7166 0.4444
Nucleus 0.4991 0.8171 0.7406 0.7753 0.4777 0.4744 0.6217 0.9173 0.8259 0.7975 0.4262 0.5609 0.4751 0.9185 0.8056 0.735 0.198 0.4567
Peroxisomes 0.0138 0.0001 0.0031 0 0.0021 0.0001 0.0101 0 0.0015 0 0.0016 0 0.0156 0 0.0036 0 0.0008 0.0016
Punctate Nuclear 0.0453 0.0017 0.004 0.0004 0.0016 0.0157 0.0189 0.0058 0.0064 0.0018 0.0256 0.0004 0.0295 0.0006 0.0035 0.0091 0.0011 0.0006
Vacuole 0.0129 0.0019 0.0169 0.0001 0.002 0.0012 0.0074 0.0003 0.0019 0.0002 0.0091 0.0022 0.0247 0.0042 0.0009 0.0069 0.0244 0.0042
Vacuole Periphery 0.018 0.0004 0.0038 0.0001 0.0042 0.0005 0.0098 0.0002 0.0041 0.0001 0.005 0.0006 0.0088 0.0018 0.001 0.0026 0.0121 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.7433 23.9848 27.4382 34.6005 25.3655 44.6927 36.1239 38.8736 39.6934 31.9761
Translational Efficiency 2.1001 2.2028 1.9366 2.0701 2.5351 1.5371 1.3432 1.5649 1.6273 1.7747

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1191 799 2727 744 812 1989 1601 713 2003 2788 4328 1457

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 849.74 1060.91 1152.93 1203.86 841.17 1063.48 1092.24 1115.52 846.27 1062.74 1130.48 1160.63
Standard Deviation 178.21 237.50 225.40 206.98 167.64 185.97 201.10 190.86 174.05 202.09 218.70 204.09
Intensity Change Log 2 — 0.320209 0.440212 0.502574 — 0.338324 0.376821 0.407247 — 0.329249 0.409025 0.455939

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000534 0.000809 0.000518 0.000896 0.000686 0.000682 0.000739 0.000648 0.000596 0.000719 0.000600 0.000775
Bud Neck 0.005264 0.007978 0.014705 0.010506 0.005725 0.012038 0.010957 0.010564 0.005451 0.010874 0.013318 0.010535
Bud Site 0.000377 0.001271 0.000842 0.000753 0.000579 0.001260 0.000833 0.000629 0.000459 0.001263 0.000838 0.000692
Cell Periphery 0.000064 0.000191 0.000075 0.000027 0.000098 0.000072 0.000045 0.000023 0.000078 0.000106 0.000064 0.000025
Cytoplasm 0.000762 0.000132 0.000108 0.000098 0.000451 0.000204 0.000326 0.000032 0.000636 0.000184 0.000188 0.000066
Cytoplasmic Foci 0.000079 0.000562 0.000020 0.000339 0.000039 0.000240 0.000027 0.000013 0.000063 0.000333 0.000023 0.000179
Eisosomes 0.000036 0.000067 0.000056 0.000055 0.000058 0.000065 0.000055 0.000060 0.000045 0.000065 0.000056 0.000057
Endoplasmic Reticulum 0.001611 0.001655 0.000845 0.001023 0.002367 0.001887 0.001240 0.000846 0.001917 0.001821 0.000991 0.000936
Endosome 0.000082 0.000792 0.000063 0.000658 0.000098 0.000562 0.000064 0.000022 0.000089 0.000628 0.000063 0.000347
Golgi 0.000079 0.000880 0.000055 0.000565 0.000078 0.000346 0.000079 0.000056 0.000078 0.000499 0.000064 0.000316
Lipid Particles 0.000107 0.000146 0.000009 0.000105 0.000039 0.000257 0.000026 0.000007 0.000079 0.000225 0.000015 0.000057
Mitochondria 0.000887 0.001483 0.001550 0.001739 0.000807 0.001349 0.001173 0.001037 0.000855 0.001387 0.001410 0.001395
Mitotic Spindle 0.001543 0.004008 0.000338 0.001750 0.001455 0.006629 0.000945 0.000052 0.001507 0.005878 0.000562 0.000919
None 0.003416 0.001543 0.000836 0.000876 0.006670 0.001005 0.002920 0.000644 0.004735 0.001160 0.001607 0.000762
Nuclear Periphery 0.000138 0.000180 0.000191 0.000124 0.000111 0.000360 0.000154 0.000048 0.000127 0.000308 0.000177 0.000087
Nuclear Periphery Foci 0.000318 0.000725 0.000049 0.000314 0.000226 0.000344 0.000075 0.000011 0.000281 0.000453 0.000059 0.000166
Nucleolus 0.056018 0.069905 0.022546 0.067001 0.064831 0.053872 0.048613 0.059669 0.059591 0.058467 0.032189 0.063413
Nucleus 0.927714* 0.904861* 0.956321* 0.910542* 0.914208* 0.917179* 0.930307* 0.924222* 0.922239* 0.913649* 0.946698* 0.917236*
Peroxisomes 0.000721 0.001547 0.000368 0.002032 0.001192 0.000895 0.001005 0.001175 0.000912 0.001082 0.000604 0.001613
Vacuole 0.000113 0.000729 0.000264 0.000395 0.000162 0.000443 0.000245 0.000125 0.000133 0.000525 0.000257 0.000263
Vacuole Periphery 0.000138 0.000532 0.000241 0.000202 0.000119 0.000311 0.000172 0.000118 0.000130 0.000375 0.000215 0.000161

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.91 -0.33 -7.61 -0.77 -7.16 0.05 -2.91 0.98 0.65 3.93 -2.42 -1.32 -6.37 -1.29 -5.15
Bud Neck -3.85 -13.59 -8.63 -3.66 4.70 -7.82 -10.08 -5.91 0.91 0.50 -9.18 -16.51 -10.08 -0.31 4.19
Bud Site -2.02 -4.25 -3.98 1.02 0.53 -3.47 -4.57 -0.74 3.16 3.55 -4.28 -5.36 -4.02 2.75 1.76
Cell Periphery -1.07 -0.42 2.33 1.39 1.92 0.83 1.75 2.55 4.54 3.67 -0.76 0.60 3.48 2.35 2.48
Cytoplasm 0.91 1.05 1.04 0.76 -0.05 1.46 0.78 2.60 3.83 4.79 1.15 1.19 1.49 2.65 3.27
Cytoplasmic Foci -1.52 1.25 -0.92 0.68 -1.20 -2.36 1.31 3.25 2.74 2.71 -2.43 1.45 -0.76 1.13 -1.14
Eisosomes -4.54 -7.40 -7.02 1.64 0.41 -1.60 0.94 -0.45 1.24 -1.49 -6.32 -5.28 -5.84 2.31 -0.56
Endoplasmic Reticulum -0.20 8.28 5.90 1.97 -2.42 2.65 9.51 13.57 7.16 7.35 0.55 12.58 13.50 6.35 1.45
Endosome -1.88 0.74 -1.26 0.42 -1.31 -1.99 1.65 6.58 2.33 3.36 -2.72 1.40 -1.07 1.17 -1.19
Golgi -1.50 1.16 -1.36 0.58 -1.42 -1.80 -0.33 2.65 1.96 6.05 -2.29 1.00 -1.28 0.92 -1.35
Lipid Particles -0.41 1.31 0.15 0.61 -1.55 -1.69 0.78 2.29 1.92 3.96 -1.47 1.42 0.55 1.80 -1.25
Mitochondria -1.73 -2.60 -5.53 -0.95 -0.69 -3.77 -6.64 -3.47 1.91 1.97 -3.51 -3.50 -5.96 -0.30 0.16
Mitotic Spindle -1.38 1.45 -0.29 1.20 -1.78 -2.52 0.57 1.47 4.50 2.30 -3.19 1.43 0.86 4.03 -0.70
None 1.85 2.69 2.58 1.57 -0.43 3.17 2.01 3.39 1.83 2.80 3.85 3.29 4.29 1.97 2.52
Nuclear Periphery -0.78 -1.16 0.11 0.96 1.55 -2.28 -1.36 3.85 2.91 3.25 -2.25 -1.64 1.35 2.80 3.16
Nuclear Periphery Foci -1.05 2.55 0.18 1.02 -0.90 -0.42 1.80 2.49 3.55 3.52 -1.03 3.14 1.11 1.76 -0.65
Nucleolus -3.21 11.84 -3.00 -0.02 -10.89 2.85 3.53 1.09 -1.40 -2.05 0.38 11.31 -1.62 -1.97 -10.53
Nucleus 3.88 -7.28 3.84 -0.30 9.65 -0.61 -2.70 -1.78 -1.37 0.72 2.40 -7.46 1.85 -0.41 8.72
Peroxisomes -3.15 5.43 -8.13 -1.76 -10.53 1.67 1.56 0.14 -1.49 -1.23 -1.36 5.15 -6.88 -3.36 -10.20
Vacuole -2.42 -2.30 -2.54 1.21 -1.03 -2.67 -1.16 2.81 3.03 1.55 -3.75 -2.47 -2.28 2.20 0.04
Vacuole Periphery -2.01 -1.54 -1.71 1.68 0.53 -4.58 -1.05 0.07 4.59 1.06 -3.82 -1.87 -1.56 3.26 1.20
Standard name
Human Ortholog
Description TFIIE small subunit; involved in RNA polymerase II transcription initiation
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Tfa2

Tfa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available