Standard name
Human Ortholog
Description Protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.97 0.98 0.89 0.79 – 0.62 0.57 0.66 0.58 0.53 0.98 0.95 0.99 0.95 0.88 0.91 0.91 0.86 0.92 0.96 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.11 0 0.16 – 0.24 0.41 0.34 0.42 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0.05 0.06 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.08 – 0.23 0.18 0.11 0.12 0.11 0 0 0 0.07 0.15 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud 0 0 2 0 2 0 – 1 1 1 1 1 2 0 0 0 0 0 0 0 0 0 3 6
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 1 1 2 – 3 3 2 0 3 0 0 0 3 0 6 0 0 0 0 0 0
Cytoplasm 200 77 159 90 144 201 – 186 185 177 158 133 242 290 333 187 101 118 191 80 160 148 222 243
Endoplasmic Reticulum 0 1 0 0 0 1 – 2 0 2 1 1 3 4 0 6 2 1 2 0 0 0 2 2
Endosome 0 0 0 0 0 0 – 2 0 1 0 0 0 0 0 0 0 0 1 0 1 0 2 3
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 3 2 1 0 1 2 0 1 0 1 4
Mitochondria 3 2 6 10 4 41 – 73 134 92 114 110 0 1 0 4 2 0 2 0 0 0 0 0
Nucleus 3 1 2 2 3 9 – 14 20 18 18 12 3 1 0 1 4 1 2 3 1 2 3 5
Nuclear Periphery 0 0 0 1 0 0 – 1 4 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 5 1 0 4 17 21 – 69 58 30 33 28 3 6 5 14 17 14 1 1 4 1 5 5
Unique Cell Count 206 80 164 92 161 253 301 327 270 274 252 247 305 335 197 115 129 210 94 175 155 248 278
Labelled Cell Count 214 82 169 108 171 275 351 405 323 325 290 253 305 340 217 126 141 210 94 175 155 248 278


Ambiguous, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 10.1 5.4 6.0 6.9 6.0 – 6.1 5.6 5.7 5.4 5.8 8.4 9.4 9.4 11.0 9.8 10.0 8.6 9.0 8.9
Std Deviation (1e-4) 1.3 1.9 1.2 1.5 1.6 1.7 – 1.7 1.6 1.3 1.5 1.4 1.6 1.6 1.8 2.2 2.7 2.4 2.2 1.6 1.8
Intensity Change (Log2) – – – 0.16 0.35 0.14 – 0.18 0.05 0.08 -0.0 0.11 0.63 0.8 0.8 1.02 0.86 0.88 0.67 0.73 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 -2.7 -5.1 – -8.3 -9.2 -7.6 -8.9 -9.6 0.7 -1.0 2.2 -1.0 -3.0 -2.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 0 4.0 – 5.7 8.6 7.3 8.6 8.9 0 0 0 0 0 0
Nucleus 0 0 0 – 1.9 2.5 2.6 2.6 2.0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.3 3.8 – 6.6 5.7 4.4 4.6 4.4 0 0 0 3.5 5.1 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1793 5.1338 4.2282 3.3979 3.978 4.3904 3.178 4.4206 3.6488 3.0331 3.187 3.6482 5.2858 5.9615 5.7687 4.8215 5.0639 5.2553
Actin 0.0231 0.0003 0.0185 0.0724 0.0408 0.0037 0.0463 0.0076 0.0034 0.006 0.0035 0.0008 0.0473 0.0008 0.0017 0.0133 0.0123 0.0087
Bud 0.0005 0.0001 0.0005 0.0002 0.0005 0.0008 0.0022 0.0092 0.0041 0.0039 0.0014 0.0002 0.0005 0.0012 0.0007 0.0003 0.0003 0.0006
Bud Neck 0.001 0.0002 0.0004 0.0004 0.001 0.0019 0.002 0.0004 0.0006 0.001 0.0006 0.0017 0.0024 0.0004 0.0012 0.0009 0.0009 0.0016
Bud Periphery 0.0004 0 0.0006 0.0001 0.0008 0.0013 0.0012 0.0045 0.0017 0.0047 0.0017 0.0001 0.0005 0.0005 0.0004 0.0002 0.0003 0.0007
Bud Site 0.005 0.0002 0.0011 0.0005 0.0024 0.0003 0.0063 0.0086 0.0076 0.0034 0.0009 0.0002 0.0031 0.0081 0.003 0.0018 0.0005 0.0005
Cell Periphery 0.0002 0 0 0 0.0002 0 0.0004 0.0001 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.8356 0.9742 0.8601 0.8635 0.7162 0.8799 0.7411 0.911 0.871 0.643 0.6432 0.883 0.7509 0.9099 0.8823 0.7996 0.8208 0.8601
Cytoplasmic Foci 0.0232 0.0013 0.0257 0.0066 0.0147 0.0104 0.0458 0.0058 0.0212 0.119 0.0137 0.01 0.0287 0.0039 0.0094 0.0134 0.0209 0.0079
Eisosomes 0.0001 0 0.0001 0.0001 0.0003 0 0.0003 0 0 0.0001 0 0 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0058 0.0016 0.0023 0.0032 0.0063 0.0032 0.0053 0.0017 0.0027 0.0057 0.0032 0.004 0.0044 0.0013 0.0025 0.0018 0.0046 0.0023
Endosome 0.0198 0.0005 0.0093 0.0035 0.0367 0.0124 0.0522 0.0017 0.017 0.0383 0.1081 0.0081 0.0135 0.0031 0.0064 0.0066 0.0382 0.0087
Golgi 0.0063 0 0.0106 0.0015 0.0202 0.0011 0.0206 0.0036 0.0068 0.0217 0.0702 0.0016 0.0086 0.0029 0.0012 0.0049 0.0084 0.0055
Lipid Particles 0.0034 0 0.0059 0.0014 0.0265 0.0003 0.0099 0.0001 0.0006 0.0017 0.0014 0.0001 0.0223 0.0011 0.0011 0.039 0.0092 0.0056
Mitochondria 0.0028 0.0001 0.0094 0.0007 0.0398 0.0012 0.0191 0.0013 0.0012 0.0031 0.0593 0.0104 0.0023 0.0034 0.0045 0.0076 0.0063 0.0031
None 0.0169 0.0072 0.0166 0.0126 0.003 0.0042 0.0146 0.0236 0.0119 0.0929 0.0344 0.023 0.025 0.0266 0.0268 0.0108 0.0052 0.0105
Nuclear Periphery 0.0115 0.0015 0.0022 0.0038 0.0317 0.0056 0.0041 0.002 0.0056 0.0052 0.014 0.007 0.0103 0.0019 0.0039 0.003 0.0046 0.0042
Nucleolus 0.0002 0 0.0001 0 0.0009 0.0002 0.0002 0.0001 0.0001 0.0004 0.0003 0.0001 0.0005 0 0.0003 0.0004 0.0002 0.0001
Nucleus 0.0283 0.012 0.0193 0.0186 0.0248 0.0665 0.0114 0.0143 0.0298 0.0254 0.0142 0.0417 0.0436 0.0326 0.0464 0.0428 0.0467 0.0722
Peroxisomes 0.0052 0 0.0144 0.0086 0.0195 0.0011 0.0093 0.0012 0.0088 0.0154 0.0007 0.0005 0.0215 0.0005 0.0038 0.0491 0.0046 0.0021
Punctate Nuclear 0.0054 0.0005 0.0021 0.0011 0.0051 0.0037 0.0039 0.0026 0.0044 0.0054 0.0013 0.0061 0.0124 0.0011 0.0032 0.0032 0.0072 0.0043
Vacuole 0.0034 0.0003 0.0006 0.0011 0.0037 0.0019 0.0028 0.0006 0.0011 0.0026 0.0143 0.001 0.0012 0.0005 0.0008 0.0008 0.0052 0.0009
Vacuole Periphery 0.0018 0.0001 0.0002 0.0002 0.0049 0.0004 0.001 0.0001 0.0005 0.0007 0.0134 0.0005 0.0005 0.0001 0.0002 0.0003 0.0034 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.2676 35.8299 37.5351 61.4274 74.8932 117.8198 57.5379 49.6215 56.3087 65.9326
Translational Efficiency 1.4263 2.5029 2.2847 1.4168 1.3272 1.0352 1.6279 2.067 1.8984 1.9164

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1994 1109 923 1308 1993 1761 2047 1551 3987 2870 2970 2859

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 816.68 870.22 1218.25 987.45 835.61 913.96 1078.21 1004.23 826.14 897.06 1121.73 996.55
Standard Deviation 107.16 139.39 180.54 141.33 117.44 133.58 142.26 166.82 112.82 137.51 168.16 155.90
Intensity Change Log 2 — 0.091609 0.576967 0.273937 — 0.129301 0.367737 0.265188 — 0.110794 0.474945 0.269519

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000080 0.000444 0.000501 0.003009 0.000118 0.000226 0.000217 0.000464 0.000099 0.000310 0.000305 0.001628
Bud Neck 0.003680 0.005523 0.003888 0.007012 0.003777 0.007300 0.010175 0.011391 0.003728 0.006613 0.008222 0.009388
Bud Site 0.002670 0.013270 0.006136 0.035937 0.003304 0.010478 0.013210 0.023863 0.002987 0.011557 0.011012 0.029387
Cell Periphery 0.000083 0.000080 0.000156 0.000166 0.000068 0.000070 0.000084 0.000138 0.000076 0.000074 0.000106 0.000151
Cytoplasm 0.670779* 0.684966* 0.455834* 0.462160* 0.660648* 0.682218* 0.567637* 0.568268* 0.665714* 0.683280* 0.532892* 0.519723*
Cytoplasmic Foci 0.100722 0.075014 0.007373 0.027661 0.102818 0.095212 0.006968 0.024643 0.101770 0.087407 0.007094 0.026023
Eisosomes 0.000046 0.000029 0.000028 0.000047 0.000040 0.000035 0.000020 0.000039 0.000043 0.000033 0.000022 0.000043
Endoplasmic Reticulum 0.000558 0.000728 0.003171 0.002103 0.000930 0.000545 0.001058 0.001196 0.000744 0.000615 0.001714 0.001611
Endosome 0.004999 0.006881 0.002470 0.022585 0.004106 0.006952 0.001740 0.012459 0.004552 0.006925 0.001967 0.017092
Golgi 0.001094 0.002490 0.000156 0.005593 0.000759 0.002224 0.000444 0.004956 0.000927 0.002327 0.000354 0.005248
Lipid Particles 0.001975 0.001490 0.001088 0.003735 0.001455 0.001518 0.000639 0.000805 0.001715 0.001507 0.000779 0.002145
Mitochondria 0.000976 0.004687 0.000501 0.017918 0.001182 0.002248 0.002483 0.004225 0.001079 0.003190 0.001867 0.010490
Mitotic Spindle 0.001933 0.015054 0.005767 0.064226 0.000405 0.007759 0.013698 0.047969 0.001169 0.010578 0.011234 0.055406
None 0.005490 0.004904 0.006811 0.002902 0.002030 0.003352 0.006910 0.008497 0.003760 0.003951 0.006880 0.005937
Nuclear Periphery 0.000301 0.000224 0.001716 0.001569 0.000250 0.000191 0.000957 0.000787 0.000276 0.000204 0.001193 0.001145
Nuclear Periphery Foci 0.000955 0.001391 0.003411 0.002353 0.000878 0.000855 0.000500 0.000934 0.000917 0.001062 0.001404 0.001584
Nucleolus 0.000747 0.001373 0.000403 0.000828 0.000352 0.000531 0.000253 0.000964 0.000549 0.000856 0.000300 0.000902
Nucleus 0.151936* 0.104234 0.399397* 0.113867 0.149098* 0.103004 0.293817* 0.140436* 0.150517* 0.103479 0.326629* 0.128280
Peroxisomes 0.001146 0.002980 0.000281 0.003906 0.001024 0.002435 0.000588 0.001947 0.001085 0.002645 0.000493 0.002843
Vacuole 0.049462 0.073177 0.100708 0.212024* 0.066492 0.071973 0.078312 0.141698 0.057975 0.072438 0.085272 0.173873
Vacuole Periphery 0.000369 0.001061 0.000202 0.010400 0.000266 0.000875 0.000291 0.004319 0.000317 0.000947 0.000263 0.007101

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -11.04 -2.79 -1.73 -1.18 -2.83 -4.05 -4.38 -2.23 -1.02 -5.03 -9.56 -3.50 -2.20 -1.68
Bud Neck -1.67 -1.96 -1.05 0.81 0.56 -3.27 -3.72 -2.88 1.20 1.44 -3.73 -4.21 -2.95 1.59 1.87
Bud Site -5.74 -4.08 -7.23 -1.79 -5.20 -5.05 -4.07 -5.52 -0.40 -1.61 -7.70 -5.45 -9.14 -1.78 -4.51
Cell Periphery 0.47 -7.81 -1.97 -2.33 5.82 -0.13 -2.67 -4.07 -3.93 -2.06 0.63 -6.74 -3.95 -4.50 2.25
Cytoplasm -0.35 37.71 30.95 26.85 -5.53 -2.43 25.98 23.68 24.75 0.57 -1.98 41.38 38.20 36.63 -0.71
Cytoplasmic Foci 5.80 29.81 26.38 17.03 -8.15 1.77 31.09 27.78 24.41 -8.35 4.61 43.06 38.31 29.67 -11.66
Eisosomes 4.71 1.50 0.75 -6.26 -1.20 2.34 7.68 0.36 -2.28 -8.76 4.94 6.47 0.92 -5.74 -8.33
Endoplasmic Reticulum -2.31 -18.41 -10.86 -7.98 11.38 2.06 -4.32 -2.23 -7.86 3.37 1.01 -14.66 -7.38 -11.48 9.23
Endosome -1.69 5.32 -1.15 0.22 -3.67 -4.11 9.51 0.77 3.85 -4.15 -4.02 11.01 -0.39 2.67 -5.85
Golgi -1.85 2.88 -0.55 1.40 -3.48 -2.80 2.61 -0.86 1.32 -1.98 -3.19 3.74 -0.98 1.89 -3.37
Lipid Particles 1.17 1.25 0.19 -0.76 -0.64 -0.11 4.67 3.44 1.40 -2.57 0.81 3.70 1.13 0.21 -1.54
Mitochondria -2.72 1.03 -2.60 -0.18 -2.92 -1.35 -0.57 -0.57 0.95 0.01 -2.95 -0.42 -2.61 0.10 -2.37
Mitotic Spindle -4.23 -3.45 -8.48 -4.01 -5.98 -4.61 -7.55 -8.61 -5.19 -4.20 -6.03 -7.80 -12.02 -6.70 -7.09
None 0.90 0.66 1.58 0.41 1.42 -2.38 -14.85 -6.50 -4.14 0.36 -0.10 -7.01 -3.64 -3.24 1.64
Nuclear Periphery 1.87 -24.96 -14.09 -14.91 3.00 2.89 -31.19 -20.47 -21.29 0.35 3.32 -38.37 -23.09 -24.46 1.38
Nuclear Periphery Foci -2.72 -9.01 -3.71 -0.59 6.40 -0.13 0.82 -1.05 -0.87 -1.94 -2.07 -5.91 -3.64 -1.37 2.88
Nucleolus -0.77 1.68 1.36 1.52 -0.79 -1.59 0.91 -1.09 0.82 -1.98 -0.99 1.96 0.95 1.71 -2.20
Nucleus 8.29 -32.90 -0.97 -7.90 30.40 10.33 -34.18 -9.69 -18.02 21.88 13.52 -46.56 -7.99 -18.75 36.19
Peroxisomes -3.09 4.23 0.25 2.91 -2.59 -4.20 3.92 2.08 5.91 -2.01 -5.03 5.66 1.31 5.74 -3.13
Vacuole -8.03 -16.70 -36.56 -30.18 -17.11 -2.00 -12.81 -27.49 -26.16 -17.00 -7.32 -21.30 -44.93 -39.74 -25.48
Vacuole Periphery -2.77 3.28 -3.52 -2.41 -3.81 -2.24 2.46 -2.24 -0.54 -2.49 -3.19 3.96 -4.15 -2.19 -4.55
Standard name
Human Ortholog
Description Protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aim29

Aim29


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim29-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available