Standard name
Human Ortholog
Description Putative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.45 0.67 0.5 0.5 0.49 0.4 0.37 0.34 0.31 0.28 0.32 0.32 0.37 0.45 0.33 0.36 0.32 0.22 0.24 0.34 0.54 0.68 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.11 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.37 0 0.09 0.1 0.24 0.4 0.28 0.51 0.39 0.63 0.48 0 0 0 0.06 0 0.07 0.06 0 0.06 0.07 0.06 0.06
Nucleus 0.4 0.34 0.27 0.39 0.32 0.26 0.33 0.38 0.3 0.38 0.29 0.35 0.6 0.54 0.37 0.48 0.38 0.46 0.3 0.36 0.23 0.09 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0.13 0.07 0.12 0.08 0.08 0.05
Vac/Vac Membrane 0.05 0.09 0 0 0.06 0.05 0 0 0.05 0 0.05 0.06 0.05 0.07 0.17 0.14 0.2 0.13 0.12 0.15 0.09 0.07 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 5 7
Bud 3 2 1 0 2 5 7 8 8 7 11 5 0 0 0 1 2 1 9 7 7 4 11 19
Bud Neck 0 0 5 7 13 3 4 16 5 5 0 1 3 2 1 2 0 0 1 0 0 2 3 4
Bud Site 0 0 0 0 2 2 7 5 8 6 7 16 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 2 0 1 0 0 1 0 0 0 1 4 1 0 0 0 0 2 2
Cytoplasm 87 52 98 147 274 198 230 214 140 129 108 133 97 163 213 188 125 157 56 31 65 109 306 421
Endoplasmic Reticulum 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 41 13 19 1 0 2 3 2 1
Endosome 4 0 0 2 18 13 10 4 1 0 0 2 8 13 26 33 38 41 10 1 2 3 5 8
Golgi 0 0 0 0 1 2 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2 0
Mitochondria 60 42 2 26 54 96 230 165 209 162 245 201 1 2 0 34 14 33 15 5 11 14 25 33
Nucleus 84 39 40 116 175 105 194 222 122 156 111 144 184 238 176 273 130 222 78 46 44 18 14 3
Nuclear Periphery 0 0 0 0 1 1 1 0 0 2 1 0 0 0 0 2 0 5 2 0 1 0 0 0
Nucleolus 3 0 0 0 3 3 9 14 12 8 11 17 1 1 0 11 3 10 8 2 3 3 2 1
Peroxisomes 1 0 0 0 1 1 1 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 2 10 28 12 27 30 19 19 5 12 5 2 7 13 6 4 33 9 23 15 37 28
Vac/Vac Membrane 11 10 6 9 32 19 15 15 19 15 18 26 15 30 79 83 70 62 30 19 18 14 18 18
Unique Cell Count 211 115 146 295 549 408 581 584 409 413 388 417 305 438 476 574 345 484 258 130 194 204 452 563
Labelled Cell Count 254 146 155 318 604 461 737 695 545 509 517 558 314 452 502 683 406 556 258 130 194 204 452 563


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.4 5.1 4.9 4.9 4.2 4.0 4.3 3.8 4.0 3.6 4.2 5.5 5.7 5.4 6.1 6.2 6.2 4.7 4.8 5.0
Std Deviation (1e-4) 0.7 0.9 1.4 1.7 1.6 1.8 1.7 1.2 1.2 1.5 1.5 7.0 1.2 1.6 1.4 1.6 1.7 1.7 1.6 1.4 1.7
Intensity Change (Log2) – – – -0.03 -0.05 -0.26 -0.33 -0.22 -0.43 -0.34 -0.49 -0.26 0.12 0.16 0.1 0.27 0.29 0.29 -0.1 -0.08 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.4 4.5 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3561 1.5359 1.0504 0.6935 0.3029 0.8203 -0.1883 0.836 0.3399 -0.228 -0.202 0.3521 -0.006 0.3664 -0.0916 0.225 -0.0074 -0.2146
Actin 0.052 0.0002 0.0183 0.0028 0.0204 0.0015 0.0147 0.0007 0.0061 0.0004 0.0143 0.0042 0.032 0.0061 0.0115 0.0241 0.0034 0.0063
Bud 0.0031 0.0007 0.0052 0.0003 0.0016 0.0004 0.0017 0.0021 0.0002 0.0011 0.005 0.0005 0.0014 0.0004 0.0007 0.0026 0.0029 0.0021
Bud Neck 0.0077 0.0009 0.0014 0.001 0.0018 0.0035 0.0037 0.0007 0.0055 0.0024 0.0014 0.0091 0.0087 0.0007 0.0018 0.0062 0.0016 0.0058
Bud Periphery 0.0059 0.0005 0.002 0.0004 0.0018 0.0007 0.0021 0.0022 0.0003 0.0014 0.0073 0.001 0.0015 0.0004 0.001 0.0039 0.0136 0.003
Bud Site 0.0106 0.0038 0.006 0.0007 0.0024 0.0005 0.0085 0.0025 0.0077 0.0004 0.0183 0.0007 0.0149 0.0022 0.0035 0.007 0.0008 0.0007
Cell Periphery 0.0016 0.0001 0.0002 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001 0.0003 0.0001 0.0007 0.0001 0.0003 0.0006 0.0002 0.0002
Cytoplasm 0.1003 0.0643 0.0664 0.1648 0.1397 0.0836 0.0686 0.0401 0.0353 0.0752 0.2564 0.0894 0.1689 0.0926 0.1087 0.2039 0.2145 0.2175
Cytoplasmic Foci 0.027 0.0036 0.0086 0.0112 0.0093 0.0061 0.0346 0.0023 0.0092 0.0406 0.0242 0.0139 0.0631 0.0016 0.0152 0.0102 0.0323 0.0121
Eisosomes 0.0007 0 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0.0004 0.0002 0.0001
Endoplasmic Reticulum 0.0072 0.0029 0.0025 0.0072 0.0036 0.0019 0.006 0.0011 0.0019 0.0015 0.0025 0.0015 0.0164 0.0014 0.0036 0.0052 0.0056 0.0106
Endosome 0.044 0.0049 0.0165 0.043 0.0156 0.0106 0.0431 0.002 0.0168 0.0137 0.0152 0.015 0.0505 0.0022 0.0268 0.022 0.0803 0.0307
Golgi 0.0113 0.0005 0.0091 0.0277 0.013 0.0022 0.0099 0.0019 0.0054 0.0018 0.0106 0.0077 0.0242 0.0002 0.008 0.0286 0.0064 0.0101
Lipid Particles 0.0249 0.0006 0.0019 0.0172 0.0034 0.0014 0.0323 0.0002 0.007 0.0147 0.0123 0.0281 0.0489 0.0004 0.0025 0.0091 0.0032 0.0059
Mitochondria 0.0252 0.0007 0.0063 0.0125 0.025 0.0142 0.0051 0.0061 0.0027 0.0014 0.0151 0.032 0.0092 0.0038 0.0051 0.0805 0.0876 0.008
None 0.2023 0.0816 0.132 0.2009 0.2734 0.2564 0.1067 0.085 0.0505 0.1364 0.2828 0.1303 0.0798 0.0507 0.0966 0.3051 0.2133 0.1004
Nuclear Periphery 0.0393 0.0125 0.0172 0.0173 0.0193 0.0123 0.0485 0.0223 0.0112 0.0116 0.013 0.0076 0.0384 0.0123 0.0155 0.0146 0.0204 0.0364
Nucleolus 0.0358 0.0179 0.0094 0.0036 0.0121 0.0167 0.0236 0.0187 0.0086 0.0067 0.0032 0.015 0.0213 0.0114 0.008 0.0026 0.0053 0.014
Nucleus 0.3112 0.7444 0.6512 0.4459 0.3758 0.525 0.5015 0.7773 0.7784 0.6128 0.2209 0.5862 0.3209 0.7808 0.6306 0.2453 0.2438 0.4854
Peroxisomes 0.0201 0.0001 0.0019 0.002 0.0016 0.0009 0.0086 0.0003 0.0098 0.0124 0.0568 0.0066 0.0267 0.0014 0.0124 0.0101 0.0032 0.001
Punctate Nuclear 0.0414 0.057 0.041 0.0351 0.0672 0.0576 0.0654 0.0316 0.0415 0.0638 0.0364 0.0461 0.0531 0.0294 0.0446 0.0101 0.053 0.0425
Vacuole 0.0229 0.0024 0.0023 0.0042 0.0105 0.0034 0.0116 0.0022 0.0013 0.0011 0.0027 0.0029 0.0158 0.0015 0.0027 0.0055 0.006 0.0039
Vacuole Periphery 0.0056 0.0003 0.0007 0.002 0.002 0.0009 0.0033 0.0007 0.0005 0.0004 0.0011 0.0019 0.0033 0.0003 0.0008 0.0026 0.0023 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1149 4.5164 6.0324 23.3303 14.4109 10.0988 5.2515 4.6231 11.4226 18.5872
Translational Efficiency 1.9986 1.0596 2.0972 1.2143 1.7204 4.5953 2.2096 2.274 2.4924 2.3956

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
58 990 284 769 1347 1635 470 1083 1405 2625 754 1852

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 733.89 798.03 856.93 873.61 689.34 740.06 923.95 908.92 691.18 761.92 898.71 894.26
Standard Deviation 188.40 108.10 114.36 148.22 89.45 104.65 139.07 145.01 95.99 109.63 134.30 147.38
Intensity Change Log 2 — 0.120879 0.223614 0.251426 — 0.102427 0.422599 0.398938 — 0.111971 0.323421 0.324758

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001674 0.001625 0.000942 0.001741 0.000154 0.000419 0.001655 0.002794 0.000217 0.000874 0.001386 0.002357
Bud Neck 0.020593 0.020735 0.008210 0.011540 0.007976 0.015791 0.006156 0.016414 0.008497 0.017656 0.006930 0.014390
Bud Site 0.058962 0.010579 0.003749 0.023960 0.001900 0.005713 0.003499 0.036655 0.004255 0.007548 0.003593 0.031383
Cell Periphery 0.000922 0.000263 0.000163 0.000377 0.000215 0.000257 0.000180 0.000359 0.000244 0.000260 0.000174 0.000367
Cytoplasm 0.125584* 0.196082* 0.103242* 0.156298* 0.223196* 0.219611* 0.103518* 0.264743* 0.219166* 0.210737* 0.103414* 0.219714*
Cytoplasmic Foci 0.088735 0.065429 0.003810 0.007143 0.038886 0.048725 0.001854 0.018856 0.040944 0.055025 0.002590 0.013993
Eisosomes 0.000202 0.000080 0.000056 0.000065 0.000050 0.000060 0.000046 0.000051 0.000056 0.000068 0.000050 0.000057
Endoplasmic Reticulum 0.019448 0.005471 0.009423 0.016135 0.001835 0.003077 0.008165 0.005760 0.002562 0.003980 0.008639 0.010068
Endosome 0.023780 0.005874 0.001447 0.004014 0.002289 0.005352 0.002672 0.021062 0.003176 0.005549 0.002210 0.013983
Golgi 0.008597 0.002979 0.000102 0.002765 0.000907 0.001972 0.002548 0.012071 0.001224 0.002352 0.001627 0.008207
Lipid Particles 0.035980 0.006847 0.001380 0.001863 0.004842 0.006241 0.001525 0.002121 0.006127 0.006469 0.001470 0.002014
Mitochondria 0.024512 0.007485 0.000970 0.003872 0.003280 0.011215 0.001929 0.007550 0.004157 0.009808 0.001568 0.006022
Mitotic Spindle 0.027586 0.010447 0.001692 0.031155 0.006896 0.012269 0.013585 0.108848* 0.007750 0.011582 0.009105 0.076588
None 0.032659 0.010149 0.005899 0.014006 0.009830 0.012620 0.011588 0.012467 0.010773 0.011688 0.009445 0.013106
Nuclear Periphery 0.006023 0.002871 0.001917 0.002886 0.002264 0.002774 0.001820 0.004238 0.002419 0.002811 0.001857 0.003677
Nuclear Periphery Foci 0.011217 0.002557 0.000997 0.004544 0.001140 0.001274 0.001727 0.004043 0.001556 0.001758 0.001452 0.004251
Nucleolus 0.046694 0.015341 0.003175 0.003819 0.007955 0.011982 0.003953 0.003388 0.009554 0.013249 0.003660 0.003567
Nucleus 0.379858* 0.604755* 0.833861* 0.652475* 0.677405* 0.621886* 0.814862* 0.404473* 0.665122* 0.615425* 0.822018* 0.507450*
Peroxisomes 0.009546 0.007574 0.000310 0.000709 0.001790 0.005462 0.000769 0.001840 0.002110 0.006258 0.000596 0.001370
Vacuole 0.057929 0.021594 0.018445 0.054195 0.006717 0.011747 0.017674 0.065782 0.008831 0.015461 0.017964 0.060971
Vacuole Periphery 0.019500 0.001262 0.000212 0.006438 0.000473 0.001552 0.000274 0.006486 0.001259 0.001442 0.000251 0.006466

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.07 0.22 -0.76 -0.83 -2.86 -4.89 -5.09 -5.69 -4.77 0.38 -3.20 -6.61 -8.96 -3.91 -0.47
Bud Neck -0.23 0.90 0.09 1.09 -2.75 -4.79 -3.89 -8.81 -3.74 -5.08 -6.68 -3.78 -9.25 -2.46 -5.68
Bud Site 2.11 2.33 1.74 -2.44 -3.77 -4.78 -4.88 -8.14 -6.25 -6.07 -2.94 -1.57 -7.67 -6.33 -7.02
Cell Periphery 2.48 2.72 2.27 -1.84 -4.22 -1.34 0.12 -0.09 1.16 -0.22 -0.56 1.21 -0.56 -0.04 -2.41
Cytoplasm -2.72 1.71 0.16 7.03 -3.47 0.54 15.52 5.78 5.58 -9.72 1.24 16.44 8.48 8.84 -9.72
Cytoplasmic Foci 1.26 4.23 4.14 12.86 -1.40 -2.76 14.91 11.06 14.77 -6.18 -3.74 15.06 12.76 19.64 -6.01
Eisosomes 3.12 3.25 3.16 0.27 -0.58 -2.48 -3.48 -4.92 -2.68 -0.02 -3.19 -3.36 -4.72 -1.91 -0.47
Endoplasmic Reticulum 1.74 0.68 -0.00 -9.68 -3.10 -4.86 -13.01 -15.13 -11.97 3.56 -4.47 -14.58 -16.36 -14.60 0.09
Endosome 3.01 3.73 3.48 4.11 -3.39 -4.68 -0.27 -4.09 -1.32 -3.67 -4.43 1.68 -3.13 0.07 -4.26
Golgi 1.28 1.94 1.68 1.68 -1.87 -1.57 -0.25 -1.88 -0.97 -1.30 -1.62 0.53 -1.27 0.05 -1.67
Lipid Particles 2.72 3.22 3.19 5.57 -1.35 -1.46 4.39 4.68 7.56 -0.45 -0.17 5.72 5.64 9.38 -0.84
Mitochondria 2.54 3.46 3.20 4.58 -4.32 -4.55 0.95 -0.59 4.72 -2.32 -4.11 2.23 0.59 6.05 -3.55
Mitotic Spindle 1.24 1.94 -1.04 -4.67 -5.92 -2.08 -2.71 -12.47 -11.68 -9.01 -2.06 -1.96 -12.99 -12.37 -10.32
None 2.18 2.63 2.22 0.33 -3.33 -1.99 0.67 0.18 2.55 -0.61 -0.57 2.78 1.08 2.19 -2.37
Nuclear Periphery 1.43 1.56 0.68 -3.89 -5.03 -1.97 -2.46 -8.62 -7.33 -6.24 -1.79 -1.99 -9.34 -8.30 -7.65
Nuclear Periphery Foci 2.09 2.54 1.39 -2.40 -4.63 -0.64 -1.75 -3.96 -3.85 -0.14 -0.95 -1.37 -5.08 -4.54 -1.61
Nucleolus 2.10 2.77 2.70 7.01 -1.46 -2.87 1.77 1.47 4.05 -0.41 -2.25 4.07 3.64 7.42 -0.89
Nucleus -4.89 -9.71 -5.84 -2.84 10.91 4.75 -9.47 12.90 8.87 20.44 4.80 -12.66 8.85 5.02 22.43
Peroxisomes 0.67 3.82 3.70 7.22 -2.59 -5.28 2.54 2.75 6.92 -0.40 -6.77 4.47 4.50 10.02 -0.74
Vacuole 2.04 1.27 -2.79 -11.06 -8.20 -5.03 -7.50 -18.18 -17.17 -12.08 -6.35 -9.01 -22.66 -20.53 -14.58
Vacuole Periphery 2.28 2.41 2.08 -1.22 -2.11 -2.80 1.92 -2.66 -0.32 -3.20 -0.43 2.83 -1.32 -1.14 -3.42
Standard name
Human Ortholog
Description Putative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (77%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Msa2

Msa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available