Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 2 | 0 | 7 | 5 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 1 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 0 | 4 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 8 | 15 | 12 | 14 | – | 27 | 12 | 7 | 6 | 6 | 6 | 12 | 21 | 9 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 0 | 1 | 2 | 2 | 3 | – | 4 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 6 | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 4 | 0 | 20 | 11 | 35 | 36 | – | 69 | 26 | 34 | 17 | 30 | 1 | 3 | 2 | 5 | 0 | 5 | 3 | 3 | 11 | 4 | 21 | 24 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 11 | 8 |
Mitochondria | 16 | 22 | 61 | 50 | 66 | 192 | – | 199 | 274 | 301 | 330 | 296 | 0 | 0 | 0 | 12 | 8 | 7 | 6 | 5 | 12 | 13 | 55 | 65 |
Nucleus | 4 | 8 | 10 | 8 | 23 | 18 | – | 37 | 7 | 19 | 26 | 20 | 9 | 6 | 10 | 23 | 4 | 10 | 5 | 4 | 3 | 1 | 0 | 0 |
Nuclear Periphery | 231 | 160 | 429 | 341 | 368 | 490 | – | 524 | 412 | 497 | 382 | 466 | 107 | 163 | 145 | 138 | 42 | 122 | 268 | 183 | 536 | 128 | 270 | 205 |
Nucleolus | 40 | 25 | 104 | 104 | 133 | 203 | – | 252 | 237 | 281 | 189 | 271 | 8 | 14 | 11 | 28 | 10 | 29 | 4 | 10 | 9 | 1 | 5 | 8 |
Peroxisomes | 2 | 0 | 1 | 0 | 1 | 12 | – | 10 | 5 | 8 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 16 | 17 |
SpindlePole | 0 | 0 | 2 | 0 | 5 | 6 | – | 4 | 2 | 6 | 3 | 2 | 3 | 6 | 5 | 2 | 0 | 0 | 1 | 0 | 4 | 0 | 5 | 4 |
Vac/Vac Membrane | 86 | 50 | 103 | 49 | 64 | 69 | – | 86 | 39 | 39 | 44 | 33 | 24 | 41 | 58 | 58 | 17 | 49 | 8 | 8 | 8 | 4 | 11 | 13 |
Unique Cell Count | 278 | 202 | 546 | 418 | 525 | 740 | 879 | 683 | 799 | 686 | 728 | 132 | 207 | 208 | 212 | 59 | 162 | 304 | 218 | 606 | 162 | 417 | 363 | |
Labelled Cell Count | 390 | 268 | 739 | 581 | 709 | 1045 | 1218 | 1017 | 1196 | 1007 | 1134 | 158 | 247 | 252 | 283 | 85 | 227 | 304 | 218 | 606 | 162 | 417 | 363 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.7 | 15.2 | 12.6 | 12.6 | 10.9 | 9.7 | – | 9.7 | 9.0 | 8.7 | 8.6 | 8.9 | 12.4 | 12.9 | 12.4 | 11.4 | 10.9 | 15.3 | 14.3 | 14.8 | 15.0 |
Std Deviation (1e-4) | 4.1 | 3.5 | 2.9 | 3.1 | 2.7 | 2.4 | – | 2.5 | 2.3 | 2.3 | 2.2 | 2.4 | 2.6 | 3.5 | 3.3 | 5.2 | 3.5 | 5.3 | 2.8 | 3.0 | 3.2 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.22 | -0.37 | – | -0.38 | -0.49 | -0.53 | -0.55 | -0.51 | -0.02 | 0.04 | -0.02 | -0.14 | -0.22 | 0.28 | 0.18 | 0.23 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | 1.0 | 0.6 | – | 1.9 | 0.4 | -1.0 | -1.0 | -1.1 | 0 | 3.3 | 5.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.9 | 2.2 | 1.0 | – | 3.2 | 0.1 | 0.5 | -1.2 | 0.4 | 0 | -1.6 | -2.0 | -0.9 | 0 | -0.3 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.4 | 0.7 | 6.6 | – | 5.4 | 11.3 | 10.8 | 13.8 | 11.6 | -4.0 | -5.0 | -5.0 | -2.3 | 0.5 | -2.6 |
Nucleus | 0.1 | 2.4 | 0.7 | – | 2.4 | -1.2 | 0.7 | 2.0 | 1.1 | 0 | 0 | 2.3 | 5.5 | 0 | 0 |
Nuclear Periphery | 1.2 | -3.2 | -4.8 | – | -7.4 | -6.8 | -6.4 | -8.4 | -5.6 | 0.6 | 0.1 | -2.6 | -3.8 | -1.3 | -0.9 |
Nucleolus | 2.2 | 2.5 | 3.5 | – | 4.1 | 6.1 | 6.4 | 3.5 | 7.0 | -3.6 | -4.1 | -4.7 | -1.9 | -0.4 | -0.3 |
Peroxisomes | 0 | 0 | 2.5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.0 | -3.0 | -5.0 | – | -4.9 | -7.2 | -8.2 | -6.7 | -8.2 | -0.2 | 0.3 | 2.7 | 2.6 | 1.8 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.512 | 11.0784 | 9.7992 | 9.9761 | 9.0001 | 10.4068 | 10.7494 | 12.5794 | 11.1763 | 11.4742 | 10.1891 | 10.7287 | 6.9765 | 13.401 | 12.9459 | 11.7646 | 10.5651 | 11.1985 |
Actin | 0.0102 | 0.0001 | 0.0239 | 0.0159 | 0.024 | 0.0001 | 0.0072 | 0.0003 | 0.0234 | 0.0015 | 0.0003 | 0 | 0.0919 | 0.0005 | 0.002 | 0.0008 | 0.0004 | 0.0003 |
Bud | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0014 | 0 | 0.0002 | 0.0009 | 0.0018 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0002 | 0.0001 | 0.0003 | 0.0007 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0 | 0.0018 | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0001 |
Bud Site | 0.002 | 0 | 0.0008 | 0.0015 | 0.0031 | 0 | 0.0006 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0.0032 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0011 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0074 | 0 | 0.0013 | 0.0001 | 0.0002 | 0 | 0.015 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0059 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.01 | 0 | 0.006 | 0.0211 | 0.0033 | 0 | 0.0248 | 0 | 0.006 | 0.0001 | 0.0149 | 0 | 0.0591 | 0 | 0.0001 | 0.0001 | 0 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.005 | 0.0005 | 0.0011 | 0.0017 | 0.0013 | 0.0002 | 0.0063 | 0.0007 | 0.0015 | 0.0014 | 0.0005 | 0.0001 | 0.0228 | 0.0013 | 0.0031 | 0.0013 | 0.0004 | 0.0003 |
Endosome | 0.0153 | 0.0001 | 0.008 | 0.0039 | 0.0034 | 0.0001 | 0.0286 | 0.0002 | 0.0073 | 0.0005 | 0.0043 | 0.0001 | 0.1205 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0083 |
Golgi | 0.0114 | 0.0001 | 0.004 | 0.0075 | 0.0032 | 0.0002 | 0.0083 | 0.0001 | 0.0053 | 0.0005 | 0.005 | 0.0001 | 0.0384 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0144 |
Lipid Particles | 0.0195 | 0.0006 | 0.0088 | 0.0085 | 0.0288 | 0.0017 | 0.0401 | 0.0028 | 0.0171 | 0.0035 | 0.2022 | 0.0014 | 0.1457 | 0.0004 | 0.0074 | 0.0019 | 0.0047 | 0.0015 |
Mitochondria | 0.0032 | 0.0009 | 0.0057 | 0.0112 | 0.017 | 0.008 | 0.005 | 0.0268 | 0.0056 | 0.0054 | 0.0083 | 0.0003 | 0.0298 | 0.003 | 0.0045 | 0.0077 | 0.001 | 0.0016 |
None | 0.0112 | 0.0001 | 0.0011 | 0.0005 | 0.0005 | 0.0001 | 0.0115 | 0.0001 | 0.0011 | 0.0006 | 0.0002 | 0 | 0.0193 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.8514 | 0.9285 | 0.8821 | 0.8454 | 0.8591 | 0.9734 | 0.783 | 0.9115 | 0.8593 | 0.9198 | 0.7154 | 0.9774 | 0.2722 | 0.9031 | 0.9082 | 0.9237 | 0.9603 | 0.9478 |
Nucleolus | 0.0033 | 0.0006 | 0.0014 | 0.0008 | 0.0013 | 0.0001 | 0.0016 | 0.0002 | 0.0006 | 0.0009 | 0.0031 | 0.0006 | 0.0042 | 0.0002 | 0.0009 | 0.0006 | 0.0004 | 0.0004 |
Nucleus | 0.0083 | 0.0207 | 0.0051 | 0.0041 | 0.0024 | 0.0009 | 0.0047 | 0.0025 | 0.003 | 0.0038 | 0.0027 | 0.0014 | 0.0095 | 0.0242 | 0.0065 | 0.0034 | 0.0016 | 0.0031 |
Peroxisomes | 0.0059 | 0 | 0.0038 | 0.0219 | 0.0036 | 0 | 0.0021 | 0 | 0.0056 | 0.0002 | 0.0027 | 0 | 0.0406 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0042 | 0.0023 | 0.0022 | 0.0012 | 0.0016 | 0.0002 | 0.0259 | 0.0003 | 0.0038 | 0.001 | 0.003 | 0.0004 | 0.0279 | 0.0003 | 0.0011 | 0.001 | 0.0006 | 0.0004 |
Vacuole | 0.0009 | 0.0004 | 0.0012 | 0.0009 | 0.002 | 0.0001 | 0.0016 | 0.0006 | 0.0009 | 0.0007 | 0.0019 | 0.0001 | 0.0239 | 0.0008 | 0.0007 | 0.0006 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0289 | 0.0449 | 0.0426 | 0.0515 | 0.0425 | 0.0148 | 0.033 | 0.0534 | 0.0557 | 0.0594 | 0.0351 | 0.0181 | 0.0795 | 0.0653 | 0.0632 | 0.0572 | 0.0298 | 0.0208 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.7196 | 42.8736 | 25.9148 | 37.0386 | 29.0323 | 29.2047 | 39.115 | 40.3023 | 29.3311 | 40.6112 |
Translational Efficiency | 0.7926 | 0.7566 | 1.0473 | 0.626 | 0.7606 | 0.8005 | 0.8266 | 0.5181 | 0.6949 | 0.6342 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1457 | 879 | 254 | 1111 | 1289 | 1071 | 1882 | 1857 | 2746 | 1950 | 2136 | 2968 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1369.26 | 1258.37 | 1517.42 | 1742.33 | 1006.71 | 1240.55 | 1516.43 | 1575.16 | 1199.08 | 1248.58 | 1516.55 | 1637.74 |
Standard Deviation | 224.32 | 244.49 | 219.00 | 342.05 | 194.76 | 212.34 | 251.41 | 315.97 | 277.93 | 227.57 | 247.78 | 335.87 |
Intensity Change Log 2 | — | -0.121840 | 0.148224 | 0.347621 | — | 0.301332 | 0.591031 | 0.645850 | — | 0.072788 | 0.352633 | 0.481575 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000344 | 0.000404 | 0.000243 | 0.000376 | 0.000918 | 0.000370 | 0.000348 | 0.000244 | 0.000613 | 0.000386 | 0.000335 | 0.000294 |
Bud Neck | 0.000636 | 0.000567 | 0.000669 | 0.003261 | 0.000804 | 0.000510 | 0.000775 | 0.000878 | 0.000715 | 0.000535 | 0.000763 | 0.001770 |
Bud Site | 0.000225 | 0.000084 | 0.000033 | 0.001060 | 0.000408 | 0.000117 | 0.000046 | 0.000105 | 0.000311 | 0.000102 | 0.000045 | 0.000462 |
Cell Periphery | 0.000043 | 0.000036 | 0.000011 | 0.000110 | 0.000111 | 0.000043 | 0.000017 | 0.000035 | 0.000075 | 0.000040 | 0.000016 | 0.000063 |
Cytoplasm | 0.000198 | 0.000434 | 0.000027 | 0.000490 | 0.002713 | 0.000165 | 0.000087 | 0.000191 | 0.001379 | 0.000287 | 0.000080 | 0.000303 |
Cytoplasmic Foci | 0.001567 | 0.001875 | 0.000458 | 0.003315 | 0.001662 | 0.001539 | 0.000362 | 0.000640 | 0.001612 | 0.001691 | 0.000373 | 0.001642 |
Eisosomes | 0.000006 | 0.000011 | 0.000004 | 0.000010 | 0.000025 | 0.000009 | 0.000005 | 0.000005 | 0.000015 | 0.000010 | 0.000005 | 0.000007 |
Endoplasmic Reticulum | 0.001241 | 0.002488 | 0.000991 | 0.001074 | 0.004443 | 0.002728 | 0.000512 | 0.000662 | 0.002744 | 0.002620 | 0.000569 | 0.000816 |
Endosome | 0.002016 | 0.000565 | 0.000142 | 0.002518 | 0.001599 | 0.000866 | 0.000094 | 0.000413 | 0.001820 | 0.000730 | 0.000100 | 0.001201 |
Golgi | 0.001108 | 0.000853 | 0.000181 | 0.003911 | 0.000537 | 0.000518 | 0.000130 | 0.001014 | 0.000840 | 0.000669 | 0.000136 | 0.002098 |
Lipid Particles | 0.000963 | 0.004113 | 0.000151 | 0.005748 | 0.007305 | 0.003460 | 0.000210 | 0.001441 | 0.003940 | 0.003754 | 0.000203 | 0.003054 |
Mitochondria | 0.004309 | 0.003964 | 0.001242 | 0.006070 | 0.012159 | 0.006892 | 0.002284 | 0.004464 | 0.007994 | 0.005572 | 0.002160 | 0.005065 |
Mitotic Spindle | 0.004806 | 0.003299 | 0.004703 | 0.029394 | 0.002903 | 0.002515 | 0.002501 | 0.005962 | 0.003913 | 0.002868 | 0.002763 | 0.014733 |
None | 0.000362 | 0.000902 | 0.000086 | 0.001045 | 0.004125 | 0.000557 | 0.000224 | 0.000327 | 0.002128 | 0.000712 | 0.000207 | 0.000596 |
Nuclear Periphery | 0.771915* | 0.645111* | 0.752823* | 0.630316* | 0.609074* | 0.692656* | 0.788955* | 0.689874* | 0.695476* | 0.671224* | 0.784659* | 0.667580* |
Nuclear Periphery Foci | 0.161853 | 0.278229* | 0.193423* | 0.240901* | 0.271836* | 0.240043* | 0.144376 | 0.231692* | 0.213480* | 0.257256* | 0.150209 | 0.235139* |
Nucleolus | 0.013479 | 0.021784 | 0.025852 | 0.011778 | 0.031187 | 0.014916 | 0.021805 | 0.010170 | 0.021791 | 0.018012 | 0.022286 | 0.010772 |
Nucleus | 0.008654 | 0.014904 | 0.012363 | 0.005438 | 0.025822 | 0.008931 | 0.013676 | 0.006679 | 0.016713 | 0.011623 | 0.013520 | 0.006215 |
Peroxisomes | 0.000359 | 0.000625 | 0.000503 | 0.000410 | 0.001082 | 0.000478 | 0.000394 | 0.000226 | 0.000699 | 0.000544 | 0.000407 | 0.000295 |
Vacuole | 0.005452 | 0.004722 | 0.000969 | 0.010569 | 0.006617 | 0.005979 | 0.002325 | 0.006578 | 0.005999 | 0.005412 | 0.002164 | 0.008072 |
Vacuole Periphery | 0.020465 | 0.015030 | 0.005126 | 0.042205 | 0.014669 | 0.016709 | 0.020874 | 0.038401 | 0.017744 | 0.015952 | 0.019002 | 0.039825 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.10 | 1.76 | -0.83 | 0.18 | -2.35 | 4.35 | 6.49 | 7.69 | 1.55 | 2.33 | 3.17 | 5.34 | 6.15 | 1.64 | 0.88 |
Bud Neck | 0.57 | -0.80 | -6.06 | -6.28 | -5.51 | 2.11 | -0.05 | -0.83 | -2.78 | -0.50 | 1.91 | -0.50 | -5.83 | -6.82 | -4.11 |
Bud Site | 2.00 | 1.19 | -2.36 | -2.74 | -2.65 | 1.59 | 1.92 | 1.59 | -0.00 | -2.30 | 2.13 | 2.59 | -0.90 | -2.72 | -3.06 |
Cell Periphery | 0.65 | 3.71 | -3.13 | -3.53 | -4.83 | 4.10 | 7.05 | 5.42 | 0.59 | -4.63 | 3.57 | 7.48 | 0.87 | -2.47 | -5.84 |
Cytoplasm | -0.92 | 3.28 | -1.81 | -0.12 | -2.62 | 2.79 | 2.89 | 2.77 | -0.44 | -3.57 | 2.27 | 3.02 | 2.38 | -0.03 | -3.19 |
Cytoplasmic Foci | -0.58 | 2.88 | -2.26 | -1.89 | -4.24 | 0.33 | 4.49 | 3.92 | 2.53 | -1.62 | -0.20 | 4.66 | -0.02 | 0.17 | -4.38 |
Eisosomes | -3.54 | 1.28 | -2.39 | 0.56 | -3.17 | 6.15 | 8.89 | 8.61 | 2.97 | 0.18 | 3.11 | 8.31 | 5.77 | 2.76 | -2.18 |
Endoplasmic Reticulum | -4.78 | 1.40 | 0.74 | 5.37 | -0.58 | 3.72 | 11.44 | 10.76 | 6.73 | -1.89 | 0.44 | 11.77 | 9.80 | 8.82 | -3.17 |
Endosome | 2.80 | 3.62 | -0.64 | -3.24 | -3.94 | 1.75 | 3.98 | 3.03 | 2.12 | -4.18 | 3.19 | 5.30 | 1.54 | -1.89 | -4.83 |
Golgi | 0.48 | 3.13 | -3.31 | -3.37 | -4.71 | 0.04 | 2.90 | -1.31 | -1.33 | -2.71 | 0.60 | 4.14 | -3.13 | -3.37 | -5.43 |
Lipid Particles | -3.47 | 4.47 | -3.91 | -1.09 | -4.60 | 3.34 | 7.24 | 5.76 | 3.10 | -4.57 | 0.25 | 7.88 | 1.28 | 0.98 | -5.87 |
Mitochondria | 0.40 | 3.83 | -1.56 | -2.00 | -4.70 | 2.85 | 6.88 | 4.76 | 1.57 | -2.48 | 2.33 | 6.95 | 2.88 | 0.40 | -4.11 |
Mitotic Spindle | 0.84 | -0.76 | -6.83 | -7.10 | -4.79 | 0.35 | 0.32 | -2.14 | -2.44 | -2.42 | 1.00 | 0.73 | -6.63 | -7.18 | -6.98 |
None | -1.35 | 3.39 | -1.11 | -0.21 | -1.57 | 4.41 | 4.87 | 4.73 | 2.01 | -2.40 | 3.35 | 5.04 | 3.43 | 0.37 | -1.69 |
Nuclear Periphery | 13.30* | 1.69 | 15.43 | 1.65 | 8.87 | -8.61 | -21.79 | -9.20 | 0.35 | 14.79 | 3.47 | -14.51 | 4.56 | 0.75 | 19.93 |
Nuclear Periphery Foci | -16.37* | -3.70 | -12.63 | 4.76 | -5.06 | 4.35 | 21.85* | 6.05 | 1.33 | -18.31* | -8.60 | 15.59 | -4.69 | 4.47 | -21.16 |
Nucleolus | -2.78 | -2.20 | 0.61 | 3.17 | 2.44 | 5.33 | 3.12 | 7.88 | 2.06 | 5.28 | 1.80 | -0.26 | 6.29 | 3.83 | 5.71 |
Nucleus | -2.60 | -1.11 | 1.63 | 3.86 | 1.96 | 5.78 | 4.12 | 7.00 | 1.30 | 3.89 | 2.79 | 1.68 | 6.63 | 3.67 | 4.54 |
Peroxisomes | -2.69 | -1.46 | -0.46 | 1.51 | 0.62 | 4.77 | 6.98 | 7.79 | 2.91 | 2.82 | 1.90 | 4.84 | 5.16 | 3.36 | 1.83 |
Vacuole | 0.63 | 5.51 | -4.01 | -4.57 | -9.32 | 0.52 | 4.50 | -0.10 | -0.66 | -5.48 | 0.69 | 5.92 | -2.56 | -3.20 | -9.16 |
Vacuole Periphery | 2.28 | 8.76 | -6.52 | -8.10 | -12.64 | -1.01 | -3.46 | -10.60 | -8.35 | -7.26 | 1.12 | -0.77 | -11.48 | -11.73 | -10.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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