Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.08 0 0 0 – 0 0.05 0.06 0.07 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 – 0 0.05 0 0.09 0 0.06 0.11 0.16 0.09 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0.08 0.08 – 0 0 0 0 0 0.06 0 0.09 0.1 0.1 0.08 0 0 0 0.07 0.05 0.08
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.06 0.08 0.08 0 0.06 – 0 0.1 0.07 0.11 0.13 0 0 0 0 0.07 0 0 0 0 0 0 0.05
Nucleus 0.06 0.05 0 0.05 0 0.06 – 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.66 0.49 0.49 0.44 0.42 0.53 – 0.52 0.49 0.45 0.53 0.48 0.57 0.47 0.49 0.63 0.68 0.69 0.05 0.08 0.05 0.06 0.05 0.06
Peroxisomes 0 0.06 0 0.16 0.24 0.18 – 0.18 0.21 0.2 0.12 0.12 0.06 0.1 0.1 0.12 0 0 0 0 0 0 0 0
SpindlePole 0.6 0.69 0.73 0.65 0.67 0.64 – 0.59 0.64 0.62 0.48 0.59 0.71 0.62 0.56 0.49 0.5 0.58 0.78 0.75 0.82 0.71 0.71 0.56
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud 1 0 1 3 5 4 – 7 9 9 4 3 0 0 0 0 1 0 3 7 2 1 3 6
Bud Neck 4 3 4 1 3 5 – 5 1 7 5 2 5 1 2 1 0 0 2 2 1 0 1 2
Bud Site 3 3 11 6 2 3 – 4 9 15 11 16 1 2 1 1 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 7 2 9 11 9 – 12 9 8 15 8 15 36 53 9 12 7 0 0 0 0 0 5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 3 1 1 0 0 0 0 0 1
Endosome 1 9 2 2 17 17 – 5 6 2 3 2 13 13 31 10 11 6 4 7 6 10 15 23
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1
Mitochondria 17 11 12 13 8 14 – 11 19 17 18 23 4 6 6 4 8 3 2 1 3 1 5 16
Nucleus 8 9 5 9 5 13 – 9 9 6 7 11 6 9 12 3 4 2 2 6 2 2 5 0
Nuclear Periphery 0 1 0 1 0 1 – 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 89 97 72 74 90 116 – 142 98 117 85 87 131 160 166 66 75 49 6 17 7 9 15 19
Peroxisomes 2 11 4 27 51 40 – 50 41 52 20 22 14 33 35 13 3 3 0 0 0 6 8 7
SpindlePole 81 136 106 110 145 141 – 161 128 163 77 108 165 212 191 51 56 41 111 158 128 112 217 174
Vac/Vac Membrane 1 3 2 0 2 0 – 2 0 0 0 0 0 1 1 6 4 1 0 1 0 0 0 2
Unique Cell Count 134 198 146 170 215 220 274 199 261 161 182 231 342 340 105 111 71 143 210 157 158 307 311
Labelled Cell Count 212 290 221 255 339 363 408 329 396 245 284 354 474 499 167 176 114 143 210 157 158 307 311


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.7 5.3 6.0 7.0 6.2 – 6.5 6.0 6.3 6.4 6.0 7.6 7.5 7.3 11.5 11.1 10.0 6.7 7.0 7.2
Std Deviation (1e-4) 1.1 1.4 1.6 2.0 2.0 2.1 – 2.0 2.4 2.0 3.0 2.0 2.4 2.2 2.3 3.6 3.2 2.9 1.9 1.5 2.1
Intensity Change (Log2) – – – 0.17 0.4 0.21 – 0.28 0.16 0.24 0.25 0.18 0.5 0.49 0.46 1.1 1.06 0.9 0.33 0.4 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.6 -3.3 -3.0 – -3.2 -1.2 -0.7 -0.2 0.4 0 0 0 -2.4 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.9 0 – 0 0 0 3.0 0 2.3 3.5 4.5 0 3.3 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 2.7 – 0 0 0 0 0 2.1 0 3.1 3.0 3.1 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 -1.8 -0.7 – -1.8 0.4 -0.6 0.9 1.3 -3.0 -3.5 -3.5 -1.4 -0.3 0
Nucleus 0.8 0 1.1 – 0 0.5 0 0.4 1.1 0 0 0.1 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.0 -1.4 0.6 – 0.5 0 -0.9 0.6 -0.3 1.4 -0.5 -0.1 2.1 2.9 2.7
Peroxisomes 3.9 5.4 4.4 – 4.5 4.9 4.8 3.2 3.1 1.5 2.6 2.8 3.0 0 0
SpindlePole -1.5 -1.0 -1.7 – -2.8 -1.6 -2.1 -4.4 -2.5 -0.2 -2.3 -3.4 -3.9 -3.6 -2.2
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2068 3.4457 3.2759 2.7081 2.6145 3.1436 2.7984 2.3116 2.6988 2.5262 2.4823 2.215 4.9903 4.9858 4.8082 4.4429 4.8297 4.3489
Actin 0.0226 0 0.0087 0.0056 0.0035 0.0018 0.0019 0 0.0014 0.0277 0.0002 0.0001 0.0035 0 0.0056 0.0015 0.0007 0.0001
Bud 0.0004 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0014 0.0002 0.0002 0.0003 0.0006 0.0005 0.0014 0.0002 0.0003 0.0019 0.0002 0.0002 0.0061 0.0002 0.0009 0.0012 0.0002 0.0002
Bud Periphery 0.0008 0 0.0001 0 0.0003 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0
Bud Site 0.0075 0.0002 0.0003 0.0001 0.0007 0.0002 0.0041 0.0002 0.0005 0.0004 0.0001 0.0001 0.0034 0.0001 0.0015 0.0003 0.0006 0
Cell Periphery 0.0004 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0017 0 0.0008 0.0001 0.0094 0.0001 0.002 0.0014 0.0003 0.0002 0.0001 0.0001 0.0008 0 0.0002 0.0004 0.0001 0
Cytoplasmic Foci 0.0187 0.0063 0.0174 0.0262 0.0192 0.0057 0.0261 0.01 0.0205 0.0174 0.0171 0.0041 0.0208 0.0037 0.0056 0.015 0.0119 0.0046
Eisosomes 0.0004 0 0 0 0 0.0001 0 0 0 0.0002 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0024 0 0.0005 0.0001 0.0061 0.0002 0.0008 0.0004 0.0002 0.0003 0.0002 0 0.0031 0 0.0002 0.0001 0.0001 0
Endosome 0.0132 0.0001 0.0133 0.0021 0.028 0.0054 0.014 0.0033 0.0076 0.0049 0.0215 0.0001 0.0055 0.0001 0.0012 0.0052 0.0133 0.0001
Golgi 0.0049 0.0001 0.0038 0.0033 0.0123 0.0047 0.0029 0.0002 0.0088 0.01 0.0185 0.0002 0.0014 0.0001 0.0009 0.0028 0.0067 0.0002
Lipid Particles 0.0335 0.004 0.0166 0.0187 0.0261 0.014 0.0198 0.0085 0.0287 0.0365 0.0588 0.0078 0.0136 0.0025 0.0023 0.0116 0.0382 0.0046
Mitochondria 0.0118 0 0.0068 0.0003 0.0036 0.0091 0.0009 0.0001 0.0019 0.0051 0.0021 0.0047 0.0008 0 0.0007 0.0004 0.0136 0.0009
None 0.0016 0 0.0006 0.0002 0.0024 0.0001 0.0026 0.0017 0.0001 0.0003 0.0001 0.0001 0.0013 0 0.0004 0.0008 0.0002 0
Nuclear Periphery 0.0048 0.0002 0.0026 0.0011 0.0045 0.0033 0.0101 0.0045 0.0048 0.0009 0.0018 0.0008 0.0184 0.0002 0.0006 0.0011 0.0014 0.0011
Nucleolus 0.1067 0.1254 0.0871 0.0222 0.0093 0.0562 0.0535 0.1396 0.0854 0.0078 0.0664 0.0644 0.077 0.147 0.0614 0.0126 0.0358 0.0578
Nucleus 0.0108 0.0408 0.0077 0.002 0.0023 0.0068 0.0114 0.0344 0.0118 0.0012 0.0038 0.0092 0.0093 0.0326 0.0155 0.0017 0.0031 0.0089
Peroxisomes 0.0153 0.0002 0.0052 0.0069 0.0074 0.0066 0.0033 0.0006 0.0064 0.0108 0.0016 0.0026 0.0064 0.0001 0.002 0.0239 0.0077 0.0008
Punctate Nuclear 0.7378 0.8225 0.8264 0.9105 0.8621 0.8842 0.8424 0.793 0.8207 0.8735 0.8054 0.9054 0.8261 0.8134 0.9004 0.9213 0.8638 0.9206
Vacuole 0.0024 0 0.0007 0.0001 0.0012 0.0004 0.0017 0.001 0.0002 0.0001 0.0008 0 0.0008 0 0.0002 0.0001 0.0011 0
Vacuole Periphery 0.001 0 0.0007 0.0001 0.0007 0.0003 0.0007 0.0008 0.0003 0.0002 0.0011 0 0.0013 0 0.0001 0.0001 0.0009 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0755 14.722 10.9244 14.1792 19.5731 43.8923 8.6832 10.0467 17.0439 16.1978
Translational Efficiency 2.1078 1.8245 2.133 2.3284 2.1697 1.3537 2.6519 1.8849 1.7472 2.5969

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
68 531 1123 91 1478 1680 2707 1707 1546 2211 3830 1798

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 876.60 861.08 1056.58 1137.76 694.85 875.57 1047.94 1042.01 702.84 872.09 1050.47 1046.86
Standard Deviation 133.75 140.00 151.81 172.66 108.24 127.55 169.97 153.24 115.66 130.79 164.90 155.71
Intensity Change Log 2 — -0.025771 0.269411 0.376206 — 0.333521 0.592783 0.584596 — 0.144211 0.421395 0.472080

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000110 0.000237 0.000073 0.000064 0.000283 0.000364 0.000058 0.000147 0.000275 0.000333 0.000062 0.000143
Bud Neck 0.001753 0.002479 0.000618 0.000711 0.002147 0.001839 0.000690 0.001523 0.002130 0.001992 0.000669 0.001482
Bud Site 0.004754 0.002150 0.000495 0.001250 0.003051 0.002312 0.000432 0.002243 0.003126 0.002273 0.000450 0.002193
Cell Periphery 0.000034 0.000055 0.000012 0.000014 0.000184 0.000089 0.000011 0.000024 0.000177 0.000081 0.000012 0.000024
Cytoplasm 0.000767 0.001292 0.000100 0.000162 0.006696 0.002491 0.000455 0.001098 0.006436 0.002203 0.000350 0.001051
Cytoplasmic Foci 0.020879 0.011761 0.001214 0.002034 0.020504 0.010121 0.001049 0.002526 0.020520 0.010515 0.001097 0.002501
Eisosomes 0.000042 0.000081 0.000014 0.000007 0.000155 0.000088 0.000010 0.000006 0.000150 0.000086 0.000011 0.000006
Endoplasmic Reticulum 0.000388 0.001125 0.000223 0.000084 0.001828 0.001203 0.000133 0.000138 0.001765 0.001184 0.000160 0.000135
Endosome 0.000907 0.002122 0.000118 0.000545 0.002378 0.003091 0.000198 0.001319 0.002313 0.002858 0.000174 0.001280
Golgi 0.001203 0.004605 0.000299 0.001397 0.003691 0.002779 0.000270 0.000919 0.003581 0.003218 0.000279 0.000943
Lipid Particles 0.001549 0.011886 0.000215 0.000112 0.009366 0.009179 0.000359 0.000843 0.009022 0.009829 0.000317 0.000806
Mitochondria 0.000166 0.001345 0.000053 0.000205 0.001167 0.001073 0.000129 0.001015 0.001123 0.001138 0.000106 0.000974
Mitotic Spindle 0.896382* 0.866700* 0.967008* 0.967279* 0.741512* 0.837946* 0.968056* 0.966366* 0.748324* 0.844852* 0.967749* 0.966412*
None 0.000341 0.000705 0.000073 0.000045 0.006867 0.001976 0.000124 0.000487 0.006580 0.001671 0.000109 0.000464
Nuclear Periphery 0.000162 0.000547 0.000110 0.000144 0.000567 0.000464 0.000191 0.000299 0.000549 0.000484 0.000167 0.000291
Nuclear Periphery Foci 0.020259 0.033272 0.003923 0.000819 0.073196 0.046076 0.002557 0.001564 0.070868 0.043001 0.002957 0.001527
Nucleolus 0.024593 0.033265 0.015362 0.010120 0.081857 0.049600 0.017276 0.010181 0.079338 0.045677 0.016715 0.010178
Nucleus 0.008956 0.012418 0.005324 0.002723 0.023816 0.017258 0.004614 0.006174 0.023162 0.016095 0.004822 0.005999
Peroxisomes 0.016375 0.012178 0.004497 0.003124 0.019304 0.011251 0.003227 0.002047 0.019175 0.011474 0.003600 0.002101
Vacuole 0.000347 0.001659 0.000240 0.002260 0.001246 0.000683 0.000127 0.000749 0.001206 0.000918 0.000160 0.000826
Vacuole Periphery 0.000035 0.000117 0.000031 0.006903 0.000186 0.000118 0.000033 0.000332 0.000179 0.000118 0.000033 0.000665

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.08 0.28 0.25 1.40 0.03 -0.71 5.21 1.36 1.53 -3.72 -0.63 4.67 1.39 1.63 -3.13
Bud Neck 0.15 1.57 1.12 1.28 -0.38 0.45 2.52 -2.45 -3.16 -4.92 -0.19 2.57 -2.28 -2.34 -4.88
Bud Site 1.35 2.24 0.94 -0.31 -1.46 0.34 2.81 -0.63 -1.11 -3.84 0.37 2.97 -0.51 -1.02 -3.86
Cell Periphery 0.27 2.44 1.75 2.67 -0.84 4.23 8.46 7.47 5.10 -2.73 4.65 8.48 7.54 5.58 -2.52
Cytoplasm 0.25 1.49 1.45 3.74 -0.31 2.92 5.12 4.06 1.69 -1.73 3.28 5.43 4.13 1.57 -2.15
Cytoplasmic Foci 0.60 2.13 1.84 3.62 -1.02 4.93 10.23 9.29 6.76 -2.05 4.75 10.43 9.47 8.05 -2.06
Eisosomes -0.03 3.29 3.51 6.06 1.07 6.56 21.74 22.60 13.80 4.41 7.25 21.60 22.81 15.62 6.18
Endoplasmic Reticulum -1.43 1.90 3.08 7.16 2.46 4.45 14.45 13.39 12.79 -1.21 4.56 13.83 13.59 14.12 0.45
Endosome -1.42 3.11 1.53 2.81 -0.78 -0.39 6.47 2.67 2.36 -3.25 -0.35 6.67 2.77 2.71 -3.32
Golgi -1.15 3.83 1.23 1.94 -1.05 3.99 15.45 11.42 6.92 -2.19 2.82 15.63 11.46 6.61 -2.19
Lipid Particles -3.39 1.41 1.49 4.62 0.99 1.44 8.64 8.19 6.33 -1.49 1.00 8.72 8.25 7.63 -1.60
Mitochondria -1.79 1.07 0.06 1.87 -1.76 0.58 3.99 0.14 -0.40 -3.27 0.33 4.10 0.16 -0.14 -3.37
Mitotic Spindle -0.61 -3.23 -3.15 -4.68 -0.53 -9.25 -25.50* -23.84* -15.98 1.39 -10.21 -25.38 -23.98 -17.05 0.48
None -0.94 1.91 2.42 5.09 2.37 5.22 7.25 6.75 3.41 -2.51 5.53 7.26 6.77 3.47 -2.47
Nuclear Periphery -1.34 1.34 0.82 2.10 -0.77 2.92 6.24 3.15 1.53 -2.05 1.72 7.98 3.23 1.76 -2.44
Nuclear Periphery Foci 0.33 2.49 2.84 8.06 3.62 6.08 20.84* 21.09* 16.50 1.40 7.02 20.69 21.24 18.20 2.87
Nucleolus 0.63 1.18 1.78 2.13 1.39 5.96 12.72 13.95 8.91 2.47 6.94 12.79 14.05 9.07 3.07
Nucleus 0.42 0.54 1.21 2.88 2.73 1.68 7.15 3.18 1.68 -3.04 2.18 6.43 3.38 1.62 -2.20
Peroxisomes 1.16 2.35 1.90 1.58 -0.38 5.73 13.54 15.26 11.39 3.25 5.88 13.27 15.21 12.47 4.44
Vacuole -1.15 -0.12 -1.05 -0.49 -1.03 1.32 3.92 -0.21 -1.11 -2.67 0.43 3.27 -0.42 -0.73 -2.50
Vacuole Periphery -2.51 -0.64 -1.00 -1.00 -1.00 1.66 3.79 -1.76 -2.37 -3.03 1.56 3.70 -1.45 -1.60 -1.77
Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
Localization
Cell Percentages spindle pole (68%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dad2

Dad2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dad2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available