Standard name | |
---|---|
Human Ortholog | |
Description | Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 12 | 2 | 4 | 8 | 13 | 9 | 17 | 29 | 10 | 7 | 5 | 24 | 31 | 11 | 6 | 14 | 8 | 1 | 7 | 3 | 6 | 6 | 5 |
Bud | 0 | 3 | 2 | 5 | 8 | 20 | 30 | 20 | 31 | 45 | 48 | 70 | 3 | 4 | 5 | 0 | 1 | 1 | 0 | 3 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 6 | 10 | 7 | 6 | 9 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 42 | 14 | 45 | 96 | 126 | 157 | 206 | 209 | 240 | 160 | 254 | 19 | 14 | 14 | 9 | 12 | 14 | 0 | 6 | 1 | 1 | 2 | 1 |
Endoplasmic Reticulum | 6 | 29 | 9 | 19 | 33 | 76 | 52 | 65 | 81 | 32 | 36 | 50 | 51 | 48 | 21 | 7 | 13 | 8 | 0 | 4 | 1 | 2 | 9 | 4 |
Endosome | 10 | 14 | 35 | 63 | 137 | 84 | 102 | 147 | 81 | 190 | 61 | 80 | 46 | 58 | 128 | 34 | 42 | 41 | 62 | 182 | 130 | 174 | 202 | 233 |
Golgi | 75 | 118 | 67 | 145 | 95 | 42 | 31 | 37 | 23 | 16 | 10 | 12 | 188 | 277 | 389 | 60 | 56 | 46 | 80 | 148 | 113 | 134 | 138 | 152 |
Mitochondria | 47 | 68 | 187 | 257 | 330 | 553 | 701 | 260 | 484 | 327 | 483 | 818 | 67 | 61 | 50 | 23 | 22 | 16 | 6 | 17 | 24 | 17 | 27 | 20 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 5 | 3 | 7 | 2 | 2 | 4 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 4 | 3 | 7 | 14 | 5 | 4 | 0 | 2 | 6 | 4 | 5 | 3 | 2 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 1 |
Nucleolus | 4 | 10 | 5 | 5 | 13 | 26 | 34 | 21 | 11 | 10 | 7 | 18 | 2 | 5 | 5 | 11 | 12 | 14 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 10 | 6 | 12 | 3 | 21 | 6 | 7 | 5 | 5 | 4 | 7 | 7 | 6 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 104 | 261 | 119 | 170 | 289 | 188 | 203 | 207 | 190 | 236 | 160 | 207 | 242 | 204 | 399 | 78 | 83 | 62 | 30 | 145 | 50 | 63 | 100 | 129 |
Unique Cell Count | 171 | 427 | 304 | 497 | 784 | 887 | 1082 | 842 | 911 | 895 | 772 | 1199 | 489 | 539 | 795 | 169 | 187 | 148 | 185 | 522 | 330 | 408 | 500 | 554 |
Labelled Cell Count | 256 | 558 | 441 | 717 | 1015 | 1155 | 1348 | 1010 | 1160 | 1136 | 991 | 1542 | 653 | 715 | 1032 | 239 | 265 | 218 | 185 | 522 | 330 | 408 | 500 | 554 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 13.3 | 8.4 | 8.6 | 7.9 | 6.8 | 6.2 | 6.9 | 6.6 | 6.2 | 6.2 | 5.8 | 11.0 | 11.2 | 11.5 | 11.8 | 11.7 | 11.3 | 12.8 | 12.5 | 12.8 |
Std Deviation (1e-4) | 2.2 | 3.1 | 1.7 | 1.6 | 1.6 | 1.5 | 1.3 | 1.4 | 1.4 | 1.4 | 1.3 | 1.2 | 1.8 | 1.9 | 1.7 | 3.4 | 3.1 | 3.6 | 2.1 | 2.4 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.09 | -0.3 | -0.43 | -0.28 | -0.34 | -0.44 | -0.43 | -0.53 | 0.4 | 0.42 | 0.46 | 0.5 | 0.49 | 0.44 | 0.62 | 0.58 | 0.62 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 1.6 | 2.4 | 0 | 0 | 0 | 3.3 | 3.7 | 0 | 0 | 4.1 | 0 |
Bud | 0 | 0 | 1.8 | 2.2 | 1.9 | 2.5 | 3.4 | 3.9 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.3 | 3.8 | 4.5 | 4.6 | 7.5 | 7.2 | 8.2 | 6.5 | 6.7 | -0.5 | -1.6 | -2.7 | 0.3 | 0.9 | 2.0 |
Endoplasmic Reticulum | 0.6 | 1.0 | 3.3 | 1.4 | 2.9 | 3.4 | 0.5 | 1.3 | 1.0 | 3.9 | 3.3 | -0.3 | 0.7 | 2.1 | 1.3 |
Endosome | 0.5 | 2.4 | -1.0 | -1.1 | 2.4 | -1.3 | 3.7 | -1.9 | -2.8 | -1.0 | -0.3 | 1.9 | 2.5 | 3.2 | 4.3 |
Golgi | 2.2 | -4.1 | -9.0 | -11.5 | -9.2 | -11.2 | -12.0 | -11.9 | -14.7 | 4.8 | 8.3 | 8.1 | 3.2 | 2.0 | 2.1 |
Mitochondria | -2.7 | -5.8 | 0.3 | 1.1 | -9.4 | -2.5 | -7.6 | 0.3 | 2.2 | -14.0 | -15.4 | -19.9 | -10.0 | -10.8 | -10.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.2 | 1.4 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.8 | 3.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | -0.7 | -6.2 | -7.4 | -4.8 | -6.3 | -4.2 | -6.2 | -8.3 | 2.8 | -0.4 | 3.3 | 1.5 | 1.1 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8622 | 8.6133 | 7.2731 | 6.6654 | 6.1511 | 7.5843 | 6.2442 | 8.4721 | 7.6147 | 7.8563 | 6.6407 | 7.6419 | 4.4448 | 7.0867 | 6.5445 | 5.1833 | 4.3183 | 6.0751 |
Actin | 0.0062 | 0.0031 | 0.0054 | 0.0039 | 0.0006 | 0.0017 | 0.0094 | 0.0001 | 0.0048 | 0.001 | 0.0037 | 0.0009 | 0.0065 | 0.0001 | 0.0032 | 0.0001 | 0.0485 | 0.0003 |
Bud | 0.0073 | 0.0048 | 0.0085 | 0.0015 | 0.0002 | 0.0046 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 |
Bud Neck | 0.0018 | 0.0003 | 0.0015 | 0.0036 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0015 | 0.0001 |
Bud Periphery | 0.0013 | 0.0013 | 0.0029 | 0.0012 | 0.0002 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0009 | 0 |
Bud Site | 0.0037 | 0.0075 | 0.0092 | 0.0021 | 0.0011 | 0.0005 | 0.0017 | 0.0001 | 0.0007 | 0.0271 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0045 | 0 |
Cell Periphery | 0.0003 | 0.0005 | 0.0011 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0008 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0051 | 0.0097 | 0.0127 | 0.0066 | 0.0009 | 0.0112 | 0.0052 | 0.0017 | 0.0018 | 0.0014 | 0.003 | 0.0008 | 0.0018 | 0.0012 | 0.0012 | 0.0006 | 0.0013 | 0.0005 |
Cytoplasmic Foci | 0.0689 | 0.0703 | 0.0969 | 0.1043 | 0.0501 | 0.0684 | 0.0451 | 0.0355 | 0.0374 | 0.0302 | 0.043 | 0.0278 | 0.0274 | 0.031 | 0.0239 | 0.0213 | 0.0304 | 0.0213 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0002 | 0 | 0.0007 | 0 |
Endoplasmic Reticulum | 0.0021 | 0.0012 | 0.0012 | 0.0014 | 0.0006 | 0.0011 | 0.0015 | 0.0016 | 0.0011 | 0.0009 | 0.0053 | 0.0012 | 0.002 | 0.0008 | 0.0011 | 0.0007 | 0.0036 | 0.0011 |
Endosome | 0.4886 | 0.5051 | 0.4413 | 0.4409 | 0.5527 | 0.4721 | 0.4907 | 0.5359 | 0.4918 | 0.5268 | 0.4498 | 0.3997 | 0.4544 | 0.5082 | 0.4347 | 0.5581 | 0.4853 | 0.3683 |
Golgi | 0.2924 | 0.3115 | 0.3115 | 0.2659 | 0.3013 | 0.359 | 0.3176 | 0.3647 | 0.3823 | 0.3393 | 0.3654 | 0.4997 | 0.3547 | 0.3821 | 0.4379 | 0.3235 | 0.3102 | 0.506 |
Lipid Particles | 0.0202 | 0.0051 | 0.0094 | 0.0227 | 0.0288 | 0.0113 | 0.0265 | 0.0032 | 0.0084 | 0.0035 | 0.0282 | 0.011 | 0.023 | 0.0127 | 0.0146 | 0.0124 | 0.0267 | 0.0228 |
Mitochondria | 0.0375 | 0.0169 | 0.026 | 0.0655 | 0.0129 | 0.0236 | 0.0509 | 0.017 | 0.0197 | 0.0057 | 0.0266 | 0.0156 | 0.0275 | 0.0123 | 0.0087 | 0.0035 | 0.0216 | 0.0176 |
None | 0.0015 | 0.0053 | 0.0049 | 0.0042 | 0.0001 | 0.001 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0034 | 0 | 0.0104 | 0 | 0.001 | 0 | 0.0012 | 0 |
Nuclear Periphery | 0.002 | 0.0006 | 0.002 | 0.0015 | 0.0004 | 0.0005 | 0.0014 | 0.0006 | 0.0012 | 0.0005 | 0.0046 | 0.0023 | 0.0045 | 0.0005 | 0.0014 | 0.0007 | 0.0077 | 0.0043 |
Nucleolus | 0.0036 | 0.0005 | 0.0068 | 0.0102 | 0.0003 | 0.0014 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0009 | 0 |
Nucleus | 0.0033 | 0.0017 | 0.0053 | 0.002 | 0.0001 | 0.0011 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0017 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0016 | 0.0001 |
Peroxisomes | 0.0203 | 0.0049 | 0.0075 | 0.0137 | 0.0116 | 0.0051 | 0.0038 | 0.0003 | 0.0062 | 0.0006 | 0.0175 | 0.002 | 0.0088 | 0.0004 | 0.0007 | 0.0005 | 0.0114 | 0.0016 |
Punctate Nuclear | 0.0017 | 0.0001 | 0.0009 | 0.0015 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0007 | 0.0001 | 0.0008 | 0.0001 | 0.0012 | 0 | 0.0003 | 0 | 0.0026 | 0.0001 |
Vacuole | 0.0133 | 0.0245 | 0.0229 | 0.0259 | 0.0252 | 0.0133 | 0.0169 | 0.0137 | 0.0183 | 0.0258 | 0.0137 | 0.0108 | 0.031 | 0.0211 | 0.0263 | 0.0333 | 0.0183 | 0.0152 |
Vacuole Periphery | 0.0185 | 0.0251 | 0.0218 | 0.0202 | 0.0126 | 0.0225 | 0.0262 | 0.0252 | 0.0246 | 0.0333 | 0.0318 | 0.0277 | 0.0429 | 0.0289 | 0.0432 | 0.0451 | 0.0202 | 0.0405 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.0128 | 31.1282 | 20.933 | 28.3511 | 14.1086 | 21.7514 | 28.5231 | 38.8342 | 28.0677 | 34.7694 |
Translational Efficiency | 1.0529 | 0.9083 | 1.1853 | 0.8607 | 1.8084 | 1.6523 | 1.2422 | 0.633 | 1.1036 | 0.9753 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
87 | 1509 | 172 | 1054 | 1629 | 794 | 505 | 2406 | 1716 | 2303 | 677 | 3460 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1037.10 | 980.62 | 1540.09 | 1176.23 | 1049.80 | 1071.57 | 1345.93 | 1189.56 | 1049.16 | 1011.98 | 1395.26 | 1185.50 |
Standard Deviation | 143.18 | 184.47 | 188.56 | 180.93 | 164.43 | 128.16 | 188.11 | 176.71 | 163.44 | 172.71 | 206.33 | 178.12 |
Intensity Change Log 2 | — | -0.080789 | 0.570460 | 0.181615 | — | 0.029612 | 0.358489 | 0.180314 | — | -0.024197 | 0.467720 | 0.180961 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000656 | 0.002022 | 0.002417 | 0.001145 | 0.000294 | 0.000164 | 0.003557 | 0.000737 | 0.000313 | 0.001381 | 0.003267 | 0.000861 |
Bud Neck | 0.000675 | 0.000274 | 0.004873 | 0.000711 | 0.000647 | 0.000254 | 0.000748 | 0.000332 | 0.000648 | 0.000267 | 0.001796 | 0.000447 |
Bud Site | 0.003386 | 0.002270 | 0.003416 | 0.007377 | 0.002071 | 0.001071 | 0.002136 | 0.002783 | 0.002137 | 0.001857 | 0.002461 | 0.004183 |
Cell Periphery | 0.000878 | 0.000110 | 0.000356 | 0.000120 | 0.000272 | 0.000178 | 0.000194 | 0.000090 | 0.000303 | 0.000133 | 0.000235 | 0.000099 |
Cytoplasm | 0.000637 | 0.000733 | 0.000247 | 0.003045 | 0.000598 | 0.000344 | 0.000400 | 0.001748 | 0.000600 | 0.000599 | 0.000361 | 0.002143 |
Cytoplasmic Foci | 0.067362 | 0.116446 | 0.051250 | 0.118791 | 0.069069 | 0.067530 | 0.057120 | 0.081144 | 0.068982 | 0.099581 | 0.055628 | 0.092613 |
Eisosomes | 0.000037 | 0.000042 | 0.000030 | 0.000021 | 0.000021 | 0.000018 | 0.000037 | 0.000012 | 0.000021 | 0.000034 | 0.000036 | 0.000015 |
Endoplasmic Reticulum | 0.005470 | 0.000571 | 0.001159 | 0.000676 | 0.000699 | 0.000888 | 0.000783 | 0.000311 | 0.000941 | 0.000680 | 0.000878 | 0.000422 |
Endosome | 0.257820* | 0.258275* | 0.161700 | 0.250604* | 0.196635 | 0.231985* | 0.160934 | 0.306167* | 0.199738 | 0.249211* | 0.161129 | 0.289241* |
Golgi | 0.284714* | 0.556503* | 0.178769* | 0.430616* | 0.534843* | 0.344820* | 0.515361* | 0.393518* | 0.522161* | 0.483521* | 0.429846* | 0.404819* |
Lipid Particles | 0.000833 | 0.012443 | 0.013299 | 0.003835 | 0.001522 | 0.002589 | 0.007455 | 0.002949 | 0.001487 | 0.009046 | 0.008940 | 0.003219 |
Mitochondria | 0.034574 | 0.013772 | 0.008279 | 0.008972 | 0.024771 | 0.041163 | 0.010098 | 0.018088 | 0.025268 | 0.023216 | 0.009636 | 0.015311 |
Mitotic Spindle | 0.004447 | 0.000419 | 0.002942 | 0.016461 | 0.000752 | 0.001158 | 0.001228 | 0.006643 | 0.000940 | 0.000674 | 0.001664 | 0.009633 |
None | 0.000057 | 0.000038 | 0.000066 | 0.000036 | 0.000052 | 0.000063 | 0.000058 | 0.000088 | 0.000052 | 0.000047 | 0.000060 | 0.000072 |
Nuclear Periphery | 0.000211 | 0.000062 | 0.005921 | 0.000379 | 0.000175 | 0.000768 | 0.000739 | 0.000581 | 0.000177 | 0.000305 | 0.002056 | 0.000520 |
Nuclear Periphery Foci | 0.000655 | 0.000478 | 0.016013 | 0.002049 | 0.000489 | 0.002366 | 0.002168 | 0.003195 | 0.000498 | 0.001129 | 0.005686 | 0.002846 |
Nucleolus | 0.000053 | 0.000025 | 0.000242 | 0.000038 | 0.000022 | 0.000093 | 0.000068 | 0.000072 | 0.000024 | 0.000048 | 0.000113 | 0.000062 |
Nucleus | 0.000098 | 0.000027 | 0.000148 | 0.000049 | 0.000265 | 0.000077 | 0.000023 | 0.000057 | 0.000257 | 0.000044 | 0.000054 | 0.000054 |
Peroxisomes | 0.002508 | 0.002082 | 0.003862 | 0.003630 | 0.000436 | 0.001670 | 0.001461 | 0.000928 | 0.000541 | 0.001940 | 0.002071 | 0.001752 |
Vacuole | 0.139840 | 0.016241 | 0.349069* | 0.100924 | 0.068010 | 0.133097 | 0.153230 | 0.108225 | 0.071652 | 0.056529 | 0.202985* | 0.106001 |
Vacuole Periphery | 0.195089* | 0.017166 | 0.195944* | 0.050519 | 0.098356 | 0.169705* | 0.082200 | 0.072331 | 0.103260* | 0.069757 | 0.111098* | 0.065687 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.42 | -1.33 | -1.17 | 1.83 | 0.97 | 1.84 | -2.83 | -1.14 | -1.49 | 2.33 | -3.81 | -3.22 | -1.98 | 1.32 | 2.51 |
Bud Neck | 2.04 | -4.63 | -0.27 | -5.03 | 4.66 | 1.01 | -0.25 | 0.78 | -1.44 | 3.22 | 1.03 | -2.58 | 0.51 | -3.44 | 5.26 |
Bud Site | 1.24 | -0.03 | -3.13 | -4.58 | -3.17 | 3.23 | -0.18 | -1.73 | -4.84 | -1.55 | 0.75 | -0.82 | -4.36 | -5.07 | -3.64 |
Cell Periphery | 2.10 | 1.40 | 2.07 | -0.63 | 3.33 | 1.00 | 0.84 | 1.97 | 3.64 | 6.37 | 1.89 | 0.74 | 2.27 | 2.76 | 6.22 |
Cytoplasm | -0.48 | 2.73 | -4.15 | -3.98 | -4.74 | 2.26 | 1.66 | -3.73 | -4.60 | -4.40 | -0.03 | 2.23 | -5.53 | -5.43 | -6.42 |
Cytoplasmic Foci | -5.04 | 1.43 | -5.02 | -0.42 | -8.85 | 0.39 | 2.73 | -4.08 | -3.39 | -5.39 | -10.25 | 3.47 | -8.43 | 2.30 | -9.53 |
Eisosomes | -0.56 | 0.75 | 1.96 | 5.91 | 1.44 | 1.01 | -3.46 | 4.85 | 3.49 | 5.28 | -4.82 | -3.48 | 3.88 | 8.18 | 5.29 |
Endoplasmic Reticulum | 1.62 | 1.42 | 1.58 | -0.89 | 1.89 | -0.83 | -0.61 | 4.15 | 2.67 | 4.11 | 1.36 | 0.31 | 2.87 | 2.82 | 4.19 |
Endosome | -0.02 | 4.08 | 0.37 | 0.99 | -6.31 | -4.39 | 4.33 | -17.09 | -8.80 | -16.87 | -8.40 | 5.21 | -15.68 | -6.88 | -17.49 |
Golgi | -9.11 | 3.19 | -4.71 | 11.51 | -13.60 | 16.34 | 1.33 | 15.76 | -4.30 | 8.51 | 4.37 | 6.72 | 14.14 | 10.56 | 2.01 |
Lipid Particles | -8.63 | -4.73 | -3.53 | 5.57 | 3.44 | -1.87 | -4.82 | -2.83 | -0.64 | 3.65 | -8.03 | -6.45 | -3.58 | 6.14 | 4.92 |
Mitochondria | 2.37 | 2.93 | 2.93 | 2.38 | -0.25 | -3.86 | 5.47 | 2.81 | 5.77 | -3.50 | 0.81 | 6.43 | 4.59 | 4.08 | -3.11 |
Mitotic Spindle | 1.11 | 0.41 | -2.80 | -6.39 | -5.25 | -0.75 | -0.98 | -6.11 | -5.72 | -5.79 | 0.62 | -1.47 | -8.19 | -9.00 | -7.82 |
None | 1.49 | -0.58 | 1.70 | 0.38 | 3.40 | -2.14 | -1.05 | -0.85 | -0.59 | -0.70 | 1.13 | -1.48 | -0.68 | -0.86 | -0.41 |
Nuclear Periphery | 3.20 | -5.19 | -2.41 | -5.70 | 5.03 | -2.20 | -6.39 | -6.82 | 0.67 | 1.55 | -1.33 | -6.27 | -7.19 | -2.15 | 5.09 |
Nuclear Periphery Foci | 0.94 | -3.57 | -2.86 | -3.29 | 3.21 | -1.80 | -6.84 | -5.31 | -0.73 | -1.87 | -1.70 | -4.58 | -6.11 | -3.31 | 2.36 |
Nucleolus | 1.29 | -3.34 | 0.61 | -1.32 | 3.75 | -1.56 | -0.97 | -2.69 | 0.35 | -0.14 | -1.40 | -2.32 | -2.81 | -0.78 | 1.20 |
Nucleus | 1.88 | -0.93 | 0.83 | -2.07 | 1.92 | 0.81 | 1.04 | 0.88 | 0.51 | -2.21 | 0.96 | 0.92 | 0.89 | -1.04 | -0.41 |
Peroxisomes | 0.47 | -0.88 | -1.11 | -2.41 | 0.16 | -2.14 | -2.94 | -4.04 | 1.26 | 1.49 | -4.70 | -3.62 | -6.10 | 0.54 | 0.72 |
Vacuole | 7.74 | -9.19 | 2.26 | -17.99 | 14.56 | -10.74 | -9.32 | -9.69 | 3.93 | 4.82 | 4.13 | -14.88 | -9.18 | -14.48 | 11.00 |
Vacuole Periphery | 7.38 | -0.03 | 5.97 | -10.46 | 10.50 | -9.21 | 2.57 | 6.03 | 13.59 | 1.79 | 7.08 | -1.18 | 9.05 | 1.22 | 7.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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