Standard name
Human Ortholog
Description Nucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0.06 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.16 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0.35 0.24 0.21 0.07 0.08 0.06 0 0.05 0 0.08 0.05 0.11 0 0 0.07 0.08 0.08 0 0 0 0 0 0
Nucleus 0.16 0.09 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.07 0.07 0.08 0.06 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.93 0.92 0.98 0.94 0.94 0.88 0.81 0.8 0.81 0.81 0.82 0.75 0.86 0.84 0.78 0.97 0.95 0.9 0.8 0.83 0.83 0.88 0.86 0.84
Peroxisomes 0 0 0 0 0.05 0.14 0.13 0.11 0.08 0.07 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.14 0.24 0.29 0.27 0.33 0.38 0.32 0.42 0 0.13 0.11 0 0 0 0.06 0 0.08 0 0 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 1 0 0 3 3 1 1 3 1 0 2 0 0 0 4 1 1 2 1 7
Bud Neck 3 2 1 5 19 10 25 18 17 18 10 16 12 17 17 0 0 0 0 2 2 1 1 3
Bud Site 0 1 1 0 3 6 17 9 21 9 6 17 0 0 0 1 0 1 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 0 0 1 6 4 9 13 9 11 9 4 3 29 40 83 2 2 5 0 0 0 1 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0
Endosome 0 0 0 2 2 2 2 3 1 2 0 1 2 3 13 1 1 0 0 2 0 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 55 58 55 44 21 23 25 21 17 18 21 16 35 9 15 7 7 7 0 0 1 1 1 2
Nucleus 38 15 8 3 7 9 12 18 15 11 12 14 23 15 6 3 6 6 18 9 6 12 13 18
Nuclear Periphery 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 223 154 221 201 298 262 366 438 290 391 222 239 288 322 400 94 83 81 192 143 197 225 318 393
Peroxisomes 2 1 5 4 15 41 59 60 30 35 30 32 4 3 13 0 0 2 1 0 1 0 1 1
SpindlePole 6 1 6 5 43 72 133 149 119 184 88 134 4 50 56 3 2 1 15 6 20 5 15 26
Vac/Vac Membrane 1 0 0 2 1 0 0 0 0 1 0 0 3 6 2 0 0 0 5 2 1 1 6 6
Unique Cell Count 240 167 226 214 318 298 452 546 359 485 272 320 333 385 515 97 87 90 239 172 237 257 369 467
Labelled Cell Count 330 234 298 272 414 434 652 729 524 679 395 475 402 465 608 111 101 105 239 172 237 257 369 467


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.3 24.4 25.1 21.2 16.6 12.6 12.1 11.7 11.2 10.4 10.1 10.3 26.3 22.3 21.2 42.3 36.2 35.7 33.2 32.3 30.6
Std Deviation (1e-4) 6.5 9.8 7.7 7.6 5.2 4.1 3.8 4.0 4.4 3.4 3.4 4.4 11.6 9.8 11.4 18.7 16.8 22.8 12.5 13.8 10.8
Intensity Change (Log2) – – – -0.24 -0.59 -0.99 -1.05 -1.09 -1.16 -1.27 -1.31 -1.29 0.07 -0.17 -0.24 0.76 0.53 0.51 0.41 0.37 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 3.4 0 3.3 2.3 2.9 2.5 0 3.0 2.4 2.8 2.3 0 0 0
Bud Site 0 0 0 2.5 0 3.3 0 0 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 4.3 4.7 6.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 -5.9 -5.3 -7.2 -8.7 -7.0 -8.4 -5.1 -6.6 -4.4 -8.6 -9.2 -3.6 -3.2 -3.3
Nucleus -1.4 -0.9 -0.3 -0.6 -0.2 0.4 -1.0 0.5 0.5 1.7 0.2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 -2.2 -4.2 -6.1 -6.3 -6.0 -6.2 -5.7 -7.3 -4.6 -5.4 -6.8 -0.5 -1.1 -3.0
Peroxisomes 0 1.5 4.6 4.6 4.0 3.1 2.7 3.8 3.6 0 0 0.3 0 0 0
SpindlePole -0.2 4.4 6.8 8.1 7.8 8.8 9.9 8.4 10.3 0 4.3 3.7 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.1455 28.1251 26.5398 24.2959 24.6635 27.2515 29.4244 34.9227 30.8995 29.3676 24.4666 30.8546 24.6079 30.5685 28.2849 27.7108 29.9699 29.0579
Actin 0.0318 0.0001 0.0001 0.0012 0 0.0052 0.0091 0.0009 0.0032 0.0101 0.0088 0.0014 0.0129 0 0.0011 0 0.0001 0
Bud 0.0003 0.0001 0.0002 0.0002 0 0 0.0005 0.0002 0.0005 0.0002 0.0015 0.0002 0.0003 0.0001 0.0003 0 0 0
Bud Neck 0.0002 0.0002 0.0001 0.0033 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0009 0.0003 0.0003 0 0.0003 0 0 0
Bud Periphery 0.0004 0.0001 0.0001 0.0004 0 0.0001 0.0007 0.0002 0.0013 0.0004 0.0045 0.0002 0.0003 0 0.0002 0 0 0
Bud Site 0.0009 0.0005 0.0004 0.0022 0.0001 0.0001 0.0016 0.001 0.0012 0.0004 0.0074 0.0002 0.0021 0.0002 0.0035 0 0 0
Cell Periphery 0.0002 0.0002 0.0003 0.0004 0.0002 0.0001 0.0006 0.0005 0.0009 0.0003 0.0009 0.0002 0.0003 0.0002 0.0003 0.0001 0.0002 0.0002
Cytoplasm 0.0008 0.0005 0.0002 0.0008 0 0.0155 0.0036 0.0001 0.0009 0 0.0021 0.0026 0.0042 0 0.0044 0 0 0
Cytoplasmic Foci 0.0116 0.0055 0.0087 0.0168 0.0141 0.0054 0.0155 0.0025 0.0115 0.0004 0.0301 0.0067 0.0479 0.0023 0.004 0.0003 0.0003 0.0029
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0009 0.0001 0.0005 0.0002 0 0 0.0002 0
Endoplasmic Reticulum 0.0011 0.0002 0 0.0002 0 0.0026 0.0015 0.0002 0.0011 0.0003 0.0021 0.0003 0.005 0 0.0021 0 0 0
Endosome 0.0033 0.0038 0.0001 0.0221 0 0.0033 0.0168 0.0011 0.0061 0.0028 0.0154 0.0018 0.0436 0 0.0155 0 0 0
Golgi 0.0015 0.0003 0.0001 0.0139 0 0.0018 0.0037 0.0006 0.0024 0.0042 0.0041 0.0011 0.0078 0 0.002 0 0 0
Lipid Particles 0.0052 0.0034 0.003 0.0259 0.0065 0.0036 0.014 0.0029 0.0049 0.0025 0.0325 0.0066 0.0206 0.0005 0.0031 0.0003 0.0012 0.0019
Mitochondria 0.0119 0.0006 0.0082 0.0056 0.0242 0.0016 0.0106 0.0062 0.0134 0.0053 0.0445 0.014 0.0135 0.0022 0.0024 0.0002 0.0887 0.0026
None 0.0027 0.0006 0.0014 0.0018 0.0001 0.004 0.0038 0.0002 0.0006 0.0001 0.0053 0.0003 0.0049 0.0005 0.0007 0 0 0.0001
Nuclear Periphery 0.001 0.0119 0.0001 0.0078 0 0.0013 0.0185 0.0039 0.0088 0.0008 0.0183 0.0014 0.017 0 0.0022 0 0 0
Nucleolus 0.8882 0.8707 0.9177 0.8102 0.9184 0.8988 0.8032 0.8541 0.8536 0.8997 0.7335 0.8997 0.7395 0.9197 0.9042 0.9416 0.8665 0.9453
Nucleus 0.0351 0.073 0.0437 0.052 0.0329 0.0534 0.0674 0.1174 0.07 0.0708 0.0576 0.0571 0.0454 0.0732 0.0474 0.0568 0.0413 0.0458
Peroxisomes 0.0015 0.0004 0.0007 0.0044 0.0015 0.0003 0.0037 0.0007 0.0019 0.0003 0.0056 0.0022 0.0107 0.0002 0.0004 0 0.0011 0.0002
Punctate Nuclear 0.0014 0.0011 0.0134 0.024 0.0004 0.0018 0.0172 0.001 0.007 0.0002 0.0156 0.002 0.0164 0.0001 0.0031 0.0001 0.0001 0.0002
Vacuole 0.0003 0.0125 0.0007 0.0039 0.0002 0.0004 0.0045 0.0018 0.005 0.0006 0.0037 0.0009 0.0034 0.0003 0.002 0.0002 0.0001 0.0002
Vacuole Periphery 0.0004 0.0143 0.0009 0.0026 0.0011 0.0005 0.0032 0.0044 0.0051 0.0005 0.0049 0.0008 0.0033 0.0002 0.0007 0.0001 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 140.5237 163.1843 130.0816 93.9351 148.8024 93.0285 107.6146 87.104 77.8416 88.8345
Translational Efficiency 2.1399 2.0906 2.0962 1.9612 1.7044 1.8548 1.7107 1.8949 1.7798 2.0705

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
Localization
Cell Percentages nucleolus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Srp40

Srp40


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srp40-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available