Standard name
Human Ortholog
Description Integral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.09 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.11 0 0.06 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.38 0.24 0.3 0.26 0.28 0.26 0.22 – 0.24 0.26 0.25 0.89 0.89 0.94 0.24 0.25 0.19 0 0.11 0 0.08 0.13 0.19
Endoplasmic Reticulum 0 0 0 0.12 0.07 0.05 0 0.05 – 0 0.05 0 0 0 0 0 0.06 0.06 0.07 0.05 0.05 0 0 0
Endosome 0.07 0.08 0.08 0.07 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0.09 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.06 0.11 0 0 0 0 0 – 0.06 0.06 0 0 0 0 0.1 0.06 0.06 0 0 0 0 0 0
Nucleus 0.14 0.12 0.18 0.12 0.1 0.12 0.18 0.18 – 0.17 0.09 0.14 0 0 0 0.24 0.25 0.23 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.64 0.38 0.53 0.37 0.59 0.55 0.55 0.6 – 0.57 0.56 0.61 0.08 0.07 0 0.47 0.46 0.59 0.72 0.62 0.76 0.67 0.62 0.55
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 1 14 6 13 8 15 – 10 7 7 5 6 2 0 0 1 2 1 5 3 3 5
Bud 1 0 2 6 1 5 6 16 – 8 9 7 1 1 1 0 0 0 17 17 11 17 17 10
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 9 11 12 0 0 0 1 4 2 0 3 5
Bud Site 1 1 0 0 2 2 0 1 – 0 0 0 1 1 1 0 0 0 – – – – – –
Cell Periphery 4 2 2 12 2 6 8 8 – 2 5 7 1 1 2 1 0 1 0 0 0 4 1 1
Cytoplasm 29 52 62 48 65 98 111 109 – 82 61 76 753 753 785 36 17 26 3 17 5 25 43 70
Endoplasmic Reticulum 5 6 7 19 17 18 17 23 – 12 11 5 5 5 5 6 4 8 17 7 14 9 11 10
Endosome 16 11 21 11 5 15 11 6 – 7 9 4 15 16 7 13 3 12 7 3 7 13 16 23
Golgi 6 1 2 1 7 2 2 2 – 5 4 4 0 0 0 5 0 2 1 0 3 0 3 4
Mitochondria 16 8 27 1 2 13 18 15 – 20 15 13 1 1 0 16 4 8 4 1 7 12 12 14
Nucleus 32 16 45 19 26 41 77 89 – 60 22 43 7 7 0 37 17 31 2 0 1 1 1 2
Nuclear Periphery 0 3 0 2 1 0 2 2 – 2 1 1 0 0 0 2 1 1 2 1 0 0 4 0
Nucleolus 4 3 2 2 2 6 19 11 – 19 8 11 0 0 0 2 3 6 0 0 0 0 0 1
Peroxisomes 0 0 2 1 1 2 8 1 – 1 0 0 0 0 0 1 1 1 1 0 0 0 0 2
SpindlePole 5 3 4 2 0 4 6 3 – 3 0 1 23 23 18 8 3 4 4 3 2 6 8 9
Vac/Vac Membrane 141 51 136 58 147 195 238 302 – 199 130 188 64 61 24 72 32 80 175 100 218 203 216 203
Unique Cell Count 221 136 255 158 248 355 433 502 347 232 310 844 848 834 153 69 135 245 162 286 305 350 370
Labelled Cell Count 263 158 313 196 284 420 531 603 430 282 367 885 886 857 199 85 181 245 162 286 305 350 370


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 8.5 8.1 12.0 13.5 13.5 16.3 17.5 – 22.6 24.3 25.8 5.2 5.2 5.2 10.4 11.1 12.4 7.6 6.9 6.9
Std Deviation (1e-4) 8.0 7.3 4.6 7.4 8.8 9.5 11.0 10.6 – 13.6 14.8 14.9 1.0 1.0 1.5 7.5 9.2 8.0 5.8 2.7 3.5
Intensity Change (Log2) – – – 0.58 0.74 0.75 1.02 1.12 – 1.49 1.6 1.68 -0.62 -0.62 -0.63 0.37 0.46 0.62 -0.08 -0.21 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 4.5 0 2.7 0 2.3 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2.1 – 0 2.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 3.7 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.5 0.9 0.4 -0.8 – -0.2 0.5 0.1 20.7 20.6 23.5 -0.2 0.1 -1.1
Endoplasmic Reticulum 3.8 2.2 1.4 0.8 1.2 – 0.5 1.2 -0.9 0 0 0 0 0 1.6
Endosome -0.5 -3.1 -2.1 -3.4 -4.9 – -3.6 -2.0 -4.0 -5.1 -4.9 -6.5 0.1 -1.1 0.2
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -3.9 -4.7 -3.4 -3.3 -4.3 – -2.2 -1.6 -2.9 -9.3 -9.3 -9.5 0 -1.2 -1.5
Nucleus -1.5 -2.3 -2.1 0 0 – -0.1 -2.6 -1.2 -11.1 -11.1 -12.4 1.6 1.3 1.3
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.7 0 – 3.1 0 2.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 1.0 1.0 0.6 0 0 0
Vacuole -3.3 1.3 0.4 0.4 1.8 – 1.0 0.6 1.7 -16.6 -16.9 -19.9 -1.2 -1.0 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2126 0.7537 0.6105 0.055 1.3948 0.6388 2.0735 1.8242 1.8525 1.301 1.7377 2.1266 2.5389 2.6803 2.0178 1.54 1.3019 1.8253
Actin 0.0436 0.0028 0.0161 0.0249 0.0078 0.0031 0.0396 0.0005 0.0153 0.001 0.0297 0.0153 0.0889 0.0001 0.0063 0.0003 0 0.0075
Bud 0.0016 0.0069 0.0019 0.0013 0.0003 0.0008 0.0029 0.0078 0.0026 0.0004 0.0016 0.0002 0.0019 0.0025 0.0005 0.0013 0.0001 0.0003
Bud Neck 0.0129 0.0016 0.0062 0.0015 0.0004 0.0006 0.0097 0.0015 0.0042 0.0001 0.0003 0.0104 0.002 0.0011 0.0009 0 0.0004 0.0038
Bud Periphery 0.0054 0.007 0.0052 0.0021 0.0003 0.0026 0.0057 0.0096 0.0052 0.0038 0.0069 0.0006 0.0084 0.0041 0.0007 0.0098 0.0001 0.001
Bud Site 0.0088 0.0147 0.0068 0.0112 0.0028 0.0002 0.0262 0.0121 0.0134 0.0003 0.0002 0.0002 0.0042 0.0055 0.0035 0.0003 0.0012 0.0003
Cell Periphery 0.0167 0.0055 0.0133 0.0007 0.0005 0.0004 0.0096 0.0022 0.0088 0.0012 0.0004 0.0009 0.0211 0.0149 0.0044 0.0018 0.0002 0.0017
Cytoplasm 0.0756 0.1144 0.0756 0.0772 0.1641 0.0499 0.0894 0.1367 0.0713 0.0652 0.1088 0.1462 0.1066 0.1545 0.1249 0.0395 0.1533 0.2636
Cytoplasmic Foci 0.061 0.0164 0.0196 0.0206 0.0556 0.0202 0.0429 0.0181 0.0312 0.007 0.0249 0.0232 0.0063 0.0119 0.0247 0.0195 0.0366 0.0247
Eisosomes 0.0005 0.0002 0.0005 0.0005 0 0.0001 0.0003 0.0002 0.0003 0.0001 0.0002 0.0001 0.0004 0.0002 0.0002 0.0005 0 0
Endoplasmic Reticulum 0.0099 0.0065 0.0029 0.0101 0.0043 0.0012 0.0247 0.0019 0.0126 0.0022 0.0017 0.0019 0.0307 0.0035 0.0015 0.0087 0.001 0.0125
Endosome 0.076 0.0344 0.0504 0.0835 0.1077 0.0765 0.0452 0.018 0.0138 0.0248 0.1345 0.0387 0.0187 0.0132 0.0232 0.0632 0.0606 0.0666
Golgi 0.0096 0.0011 0.0053 0.0082 0.006 0.0026 0.0166 0.0016 0.0043 0.0011 0.01 0.002 0.011 0.0005 0.001 0.0086 0.001 0.0047
Lipid Particles 0.0232 0.0014 0.0113 0.019 0.0452 0.0133 0.0252 0.0037 0.0156 0.0111 0.0086 0.0087 0.005 0.0166 0.0033 0.0064 0.003 0.0188
Mitochondria 0.0067 0.0011 0.0063 0.006 0.0006 0.0036 0.0051 0.001 0.0017 0.0017 0.0023 0.0011 0.0066 0.0007 0.0005 0.2068 0.0005 0.001
None 0.5307 0.6701 0.7215 0.5704 0.3365 0.7313 0.569 0.7035 0.7796 0.7746 0.5451 0.6732 0.5335 0.666 0.7643 0.5862 0.666 0.5399
Nuclear Periphery 0.0175 0.0034 0.0038 0.0066 0.0037 0.0033 0.0028 0.0007 0.0013 0.0009 0.0018 0.0006 0.014 0.0013 0.0009 0.0035 0.0005 0.0014
Nucleolus 0.0016 0.0024 0.0014 0.0031 0.0022 0.0011 0.0009 0.0011 0.0006 0.0002 0.0003 0.0012 0.0101 0.0019 0.0009 0.0007 0.0002 0.0002
Nucleus 0.0105 0.0088 0.0058 0.0045 0.0047 0.0017 0.002 0.0043 0.0012 0.0007 0.0009 0.0012 0.0255 0.0025 0.0009 0.0012 0.0004 0.0007
Peroxisomes 0.0233 0.0005 0.0041 0.0037 0.0136 0.0076 0.0162 0.0137 0.0094 0.0576 0.0016 0.0027 0.0089 0.0003 0.0028 0.0016 0.0002 0.0028
Punctate Nuclear 0.0187 0.0025 0.0037 0.005 0.0143 0.0022 0.0017 0.0033 0.0023 0.0004 0.0004 0.0005 0.0009 0.0011 0.0017 0.0002 0.0002 0.0004
Vacuole 0.0372 0.0937 0.0355 0.1306 0.2163 0.0653 0.0617 0.0572 0.0049 0.0432 0.1065 0.0661 0.0879 0.0916 0.032 0.0266 0.0722 0.044
Vacuole Periphery 0.009 0.0046 0.0028 0.0093 0.0131 0.0123 0.0026 0.0012 0.0005 0.0026 0.0134 0.0049 0.0072 0.0062 0.0011 0.0133 0.0022 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.1175 122.8874 163.7327 238.6338 212.2946 61.0628 99.6772 122.0647 136.9668 149.0274
Translational Efficiency 1.2803 1.2977 1.4619 1.3287 1.3629 1.5117 1.1825 1.1999 1.3388 1.1755

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Integral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
Localization
Cell Percentages vacuole (12%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ptr2

Ptr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available